                         SEQUENCE LISTING

<110>  Second Genome, Inc.
 
<120>  LACTOCOCCUS LACTIS EXPRESSION SYSTEM FOR DELIVERING PROTEINS 
       EFFICACIOUS FOR THE TREATMENT OF EPITHELIAL BARRIER FUNCTION 
       DISORDERS

<130>  47192-0028WO1

<150>  US 62/743,372
<151>  2018-10-09

<160>  90    

<170>  PatentIn version 3.5

<210>  1
<211>  256
<212>  PRT
<213>  Rosburia hominis

<400>  1

Met Lys Arg Leu Val Cys Thr Val Cys Ser Val Leu Leu Cys Ala Gly 
1               5                   10                  15      


Leu Leu Ser Gly Cys Gly Thr Ser Leu Glu Gly Glu Glu Ser Val Val 
            20                  25                  30          


Tyr Val Gly Lys Lys Gly Val Ile Ala Ser Leu Asp Val Glu Thr Leu 
        35                  40                  45              


Asp Gln Ser Tyr Tyr Asp Glu Thr Glu Leu Lys Ser Tyr Val Asp Ala 
    50                  55                  60                  


Glu Val Glu Asp Tyr Thr Ala Glu His Gly Lys Asn Ala Val Lys Val 
65                  70                  75                  80  


Glu Ser Leu Lys Val Glu Asp Gly Val Ala Lys Leu Lys Met Lys Tyr 
                85                  90                  95      


Lys Thr Pro Glu Asp Tyr Thr Ala Phe Asn Gly Ile Glu Leu Tyr Gln 
            100                 105                 110         


Gly Lys Val Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp Gly Glu 
        115                 120                 125             


Phe Ala Arg Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr Lys Gln 
    130                 135                 140                 


Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg Ala Asn 
145                 150                 155                 160 


Thr Asp Val Lys Val Asp Gly Glu Ile Cys Tyr Val Ser Cys Gln Asn 
                165                 170                 175     


Val Lys Leu Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly Tyr Tyr 
            180                 185                 190         


Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly Gln Glu 
        195                 200                 205             


Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu Met Thr 
    210                 215                 220                 


Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala Ala Ala 
225                 230                 235                 240 


Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile Val Tyr Lys 
                245                 250                 255     


<210>  2
<211>  768
<212>  DNA
<213>  Rosburia hominis

<400>  2
atgaagagat tagtgtgcac ggtctgcagt gtactgttgt gtgcgggact tctctccgga       60

tgcggtacct cgctggaggg agaggaaagt gtcgtgtacg tgggaaagaa aggcgtgata      120

gcgtcgctgg atgtggagac gctcgatcag tcctactacg atgagacgga actgaagtcc      180

tatgtggatg cagaggtgga agattacacc gcggagcatg gtaaaaatgc agtcaaggtg      240

gagagcctta aggtggaaga cggtgtggcg aagcttaaga tgaagtacaa gacaccggag      300

gattataccg catttaatgg aattgaactc tatcagggga aagtcgttgc ttccctggcg      360

gcaggatacg tctacgacgg ggagttcgcc cgcgtggagg aaggcaaggt tgtgggagct      420

gccacaaaac aggatattta ctctgaggat gatttgaaag ttgccatcat ccgtgccaat      480

acggatgtga aggtggacgg tgagatctgc tatgtctcct gtcagaatgt gaagctgacc      540

ggaaaagaca gtgtgtcgat ccgtgacgga tattatcttg agacgggaag cgtgacggca      600

tccgtggatg tgaccggaca ggagagcgtc gggaccgagc agctttcggg aaccgaacag      660

atggagatga ccggggagcc ggtgaatgcg gatgataccg agcagacaga ggcggcggcc      720

ggtgacggtt cgttcgagac agacgtatat actttcattg tctacaaa                   768


<210>  3
<211>  232
<212>  PRT
<213>  Rosburia hominis

<400>  3

Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val Ile 
1               5                   10                  15      


Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu Thr 
            20                  25                  30          


Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala Glu 
        35                  40                  45              


His Gly Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp Gly 
    50                  55                  60                  


Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr Ala 
65                  70                  75                  80  


Phe Asn Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu Ala 
                85                  90                  95      


Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly Lys 
            100                 105                 110         


Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp Leu 
        115                 120                 125             


Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly Glu 
    130                 135                 140                 


Ile Cys Tyr Val Ser Cys Gln Asn Val Lys Leu Thr Gly Lys Asp Ser 
145                 150                 155                 160 


Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala 
                165                 170                 175     


Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu Ser 
            180                 185                 190         


Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp Asp 
        195                 200                 205             


Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp 
    210                 215                 220                 


Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230         


<210>  4
<211>  696
<212>  DNA
<213>  Rosburia hominis

<400>  4
ttggagggag aggaaagtgt cgtgtacgtg ggaaagaaag gcgtgatagc gtcgctggat       60

gtggagacgc tcgatcagtc ctactacgat gagacggaac tgaagtccta tgtggatgca      120

gaggtggaag attacaccgc ggagcatggt aaaaatgcag tcaaggtgga gagccttaag      180

gtggaagacg gtgtggcgaa gcttaagatg aagtacaaga caccggagga ttataccgca      240

tttaatggaa ttgaactcta tcaggggaaa gtcgttgctt ccctggcggc aggatacgtc      300

tacgacgggg agttcgcccg cgtggaggaa ggcaaggttg tgggagctgc cacaaaacag      360

gatatttact ctgaggatga tttgaaagtt gccatcatcc gtgccaatac ggatgtgaag      420

gtggacggtg agatctgcta tgtctcctgt cagaatgtga agctgaccgg aaaagacagt      480

gtgtcgatcc gtgacggata ttatcttgag acgggaagcg tgacggcatc cgtggatgtg      540

accggacagg agagcgtcgg gaccgagcag ctttcgggaa ccgaacagat ggagatgacc      600

ggggagccgg tgaatgcgga tgataccgag cagacagagg cggcggccgg tgacggttcg      660

ttcgagacag acgtatatac tttcattgtc tacaaa                                696


<210>  5
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SG-11 expressed in pGEX6 vector and cleaved by Precision protease

<400>  5

Gly Pro Leu Gly Ser Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly 
1               5                   10                  15      


Lys Lys Gly Val Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser 
            20                  25                  30          


Tyr Tyr Asp Glu Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu 
        35                  40                  45              


Asp Tyr Thr Ala Glu His Gly Lys Asn Ala Val Lys Val Glu Ser Leu 
    50                  55                  60                  


Lys Val Glu Asp Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro 
65                  70                  75                  80  


Glu Asp Tyr Thr Ala Phe Asn Gly Ile Glu Leu Tyr Gln Gly Lys Val 
                85                  90                  95      


Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg 
            100                 105                 110         


Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr 
        115                 120                 125             


Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val 
    130                 135                 140                 


Lys Val Asp Gly Glu Ile Cys Tyr Val Ser Cys Gln Asn Val Lys Leu 
145                 150                 155                 160 


Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr 
                165                 170                 175     


Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly 
            180                 185                 190         


Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro 
        195                 200                 205             


Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly 
    210                 215                 220                 


Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile Val Tyr Lys Ala Ala Ala 
225                 230                 235                 240 


Ser 
    


<210>  6
<211>  723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG-11 expressed in pGEX6 vector and cleaved by Precision protease

<400>  6
gggcccctgg gatccctgga gggagaggaa agtgtcgtgt acgtgggaaa gaaaggcgtg       60

atagcgtcgc tggatgtgga gacgctcgat cagtcctact acgatgagac ggaactgaag      120

tcctatgtgg atgcagaggt ggaagattac accgcggagc atggtaaaaa tgcagtcaag      180

gtggagagcc ttaaggtgga agacggtgtg gcgaagctta agatgaagta caagacaccg      240

gaggattata ccgcatttaa tggaattgaa ctctatcagg ggaaagtcgt tgcttccctg      300

gcggcaggat acgtctacga cggggagttc gcccgcgtgg aggaaggcaa ggttgtggga      360

gctgccacaa aacaggatat ttactctgag gatgatttga aagttgccat catccgtgcc      420

aatacggatg tgaaggtgga cggtgagatc tgctatgtct cctgtcagaa tgtgaagctg      480

accggaaaag acagtgtgtc gatccgtgac ggatattatc ttgagacggg aagcgtgacg      540

gcatccgtgg atgtgaccgg acaggagagc gtcgggaccg agcagctttc gggaaccgaa      600

cagatggaga tgaccgggga gccggtgaat gcggatgata ccgagcagac agaggcggcg      660

gccggtgacg gttcgttcga gacagacgta tatactttca ttgtctacaa agcggccgca      720

tcg                                                                    723


<210>  7
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SG-11 with start methionine

<400>  7

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Asn Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Cys Tyr Val Ser Cys Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  8
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG-11 with start methionine

<400>  8
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaaaatg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcaatg gtatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagatttg ctacgttagc tgtcaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  9
<211>  246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SG-11 with N-terminal FLAG tag

<400>  9

Met Asp Tyr Lys Asp Asp Asp Asp Lys Gly Ser Ser His Met Leu Glu 
1               5                   10                  15      


Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val Ile Ala Ser 
            20                  25                  30          


Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu Thr Glu Leu 
        35                  40                  45              


Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala Glu His Gly 
    50                  55                  60                  


Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp Gly Val Ala 
65                  70                  75                  80  


Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr Ala Phe Asn 
                85                  90                  95      


Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu Ala Ala Gly 
            100                 105                 110         


Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly Lys Val Val 
        115                 120                 125             


Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val 
    130                 135                 140                 


Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly Glu Ile Cys 
145                 150                 155                 160 


Tyr Val Ser Cys Gln Asn Val Lys Leu Thr Gly Lys Asp Ser Val Ser 
                165                 170                 175     


Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val 
            180                 185                 190         


Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr 
        195                 200                 205             


Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu 
    210                 215                 220                 


Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr 
225                 230                 235                 240 


Thr Phe Ile Val Tyr Lys 
                245     


<210>  10
<211>  738
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG-11 with N-terminal FLAG tag (not codon optimized)

<400>  10
atggactaca aagacgatga cgacaagggc agcagccata tgctggaggg agaggaaagt       60

gtcgtgtacg tgggaaagaa aggcgtgata gcgtcgctgg atgtggagac gctcgatcag      120

tcctactacg atgagacgga actgaagtcc tatgtggatg cagaggtgga agattacacc      180

gcggagcatg gtaaaaatgc agtcaaggtg gagagcctta aggtggaaga cggtgtggcg      240

aagcttaaga tgaagtacaa gacaccggag gattataccg catttaatgg aattgaactc      300

tatcagggga aagtcgttgc ttccctggcg gcaggatacg tctacgacgg ggagttcgcc      360

cgcgtggagg aaggcaaggt tgtgggagct gccacaaaac aggatattta ctctgaggat      420

gatttgaaag ttgccatcat ccgtgccaat acggatgtga aggtggacgg tgagatctgc      480

tatgtctcct gtcagaatgt gaagctgacc ggaaaagaca gtgtgtcgat ccgtgacgga      540

tattatcttg agacgggaag cgtgacggca tccgtggatg tgaccggaca ggagagcgtc      600

gggaccgagc agctttcggg aaccgaacag atggagatga ccggggagcc ggtgaatgcg      660

gatgataccg agcagacaga ggcggcggcc ggtgacggtt cgttcgagac agacgtatat      720

actttcattg tctacaaa                                                    738


<210>  11
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-11  (C147V, C151S)

<400>  11

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Asn Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Val Tyr Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  12
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-11  (C147V, C151S) (codon optimized)

<400>  12
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaaaatg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcaatg gtatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagattgt gtacgttagc agccaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  13
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-11  (G84D, C147V, C151S)

<400>  13

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Asn Asp Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Val Tyr Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  14
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-11  (G84D, C147V, C151S) (codon 
       optimized)

<400>  14
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaaaatg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcaatg acatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagattgt gtacgttagc agccaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  15
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-11  (N83S, C147V, C151S)

<400>  15

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Asn Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Ser Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Val Tyr Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  16
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-11  (N83S, C147V, C151S) (codon 
       optimized)

<400>  16
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaaaatg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcagcg gtatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagattgt gtacgttagc agccaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  17
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-11  (N53S, G84D, C147V, C151S)

<400>  17

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Ser Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Asn Asp Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Val Tyr Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  18
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-11  (N53S, G84D, C147V, C151S) (codon 
       optimized)

<400>  18
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaatccg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcaatg acatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagattgt gtacgttagc agccaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  19
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-11  (N53S, N83S, C147V, C151S)

<400>  19

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Ser Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Ser Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Val Tyr Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  20
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-11  (N53S, N83S, C147V, C151S) (codon 
       optimized)

<400>  20
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaatccg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcagcg gtatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagattgt gtacgttagc agccaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  21
<211>  227
<212>  PRT
<213>  Roseburia intestinalis

<400>  21

Met Leu Asp Ala Asp Thr Asp Thr Val Tyr Val Gln Lys Asn Gly Thr 
1               5                   10                  15      


Val Leu Ser Val Asp Val Glu Thr Leu Asp Lys Asp Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Asp Tyr Val Thr Asp Ala Val Ser Thr Tyr Thr Gly 
        35                  40                  45              


Glu His Gly Lys Ser Ala Val Lys Leu Glu Asn Leu Ser Val Lys Asp 
    50                  55                  60                  


Gly Thr Ala Thr Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Gly Phe Asn Gly Ile Glu Leu Tyr Glu Gly Lys Val Val Lys Ala Leu 
                85                  90                  95      


Ala Ala Gly Tyr Asp Phe Lys Thr Asp Phe Val Ser Val Glu Asp Gly 
            100                 105                 110         


Lys Val Thr Gly Thr Ala Thr Lys Glu Glu Ile Tyr Ser Gly Glu Asp 
        115                 120                 125             


Leu Lys Val Val Ile Ile Lys Ala Asn Arg Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Thr Ile Cys Tyr Val Ser Ser Glu Asn Val Lys Leu Thr Gly Thr Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Ser Leu Asn Ser Gly Ser Thr Ala 
                165                 170                 175     


Asp Glu Ser Asp Ser Asp Glu Asn Ile Ala Asp Gly Thr Glu Ser Ile 
            180                 185                 190         


Gly Gly Ser Thr Glu Val Ser Asp Thr Asp Val Asn Asp Asp Thr Thr 
        195                 200                 205             


Tyr Val Lys Asp Asp Gly Ala Phe Glu Thr Asp Val Tyr Thr Tyr Ile 
    210                 215                 220                 


Ile Tyr Lys 
225         


<210>  22
<211>  215
<212>  PRT
<213>  Roseburia sp. 831b

<400>  22

Met Leu Asp Val Glu Glu Ser Thr Val Tyr Val Gln Lys Asn Gly Ser 
1               5                   10                  15      


Val Ile Ser Thr Asp Ile Glu Asp Phe Ser Ala Asp Tyr Tyr Asp Glu 
            20                  25                  30          


Asp Glu Leu Lys Asp Tyr Ile Gly Asp Glu Ile Ser Ser Tyr Thr Ser 
        35                  40                  45              


Glu Asn Gly Lys Lys Ser Val Ser Leu Glu Ser Val Ser Val Lys Asp 
    50                  55                  60                  


Ser Val Ala Lys Leu Thr Met Lys Tyr Lys Thr Ala Glu Asp Tyr Thr 
65                  70                  75                  80  


Asn Phe Asn Gly Val Glu Leu Tyr Thr Gly Thr Ile Val Lys Ala Met 
                85                  90                  95      


Ala Ala Gly Tyr Asp Phe Gly Val Asp Phe Val Ser Val Lys Asp Gly 
            100                 105                 110         


Ala Val Thr Gly Thr Ala Thr Lys Asp Glu Ile Val Asp His Asp Asp 
        115                 120                 125             


Tyr Lys Val Ala Val Ile Lys Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Thr Ile Val Tyr Val Ser Ser Gln Asn Val Lys Val Thr Gly Lys Asn 
145                 150                 155                 160 


Thr Val Ser Ile Arg Glu Gly Tyr Leu Ala Ala Asp Thr Thr Asn Val 
                165                 170                 175     


Val Gly Ser Thr Glu Thr Val Ala Glu Thr Glu Ala Glu Glu Ala Asn 
            180                 185                 190         


Gln Thr Glu Ala Val Leu Glu Asp Glu Phe Ala Ser Glu Ser Asp Val 
        195                 200                 205             


Tyr Thr Tyr Val Ile Phe Lys 
    210                 215 


<210>  23
<211>  236
<212>  PRT
<213>  Roseburia inulinivorans

<400>  23

Met Leu Glu Ala Asp Thr Asn Thr Val Tyr Val Ser Lys His Gly Lys 
1               5                   10                  15      


Val Val Ser Met Asp Val Glu Gln Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Glu Phe Val Asp Ser Ala Val Asp Glu Tyr Asn Thr 
        35                  40                  45              


Glu Asn Gly Lys Asn Ser Val Lys Val Asp Asp Leu Thr Val Glu Asp 
    50                  55                  60                  


Gly Thr Ala Lys Leu Arg Met Asp Tyr Glu Thr Val Asp Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Asn Gly Val Glu Leu Tyr Glu Gly Lys Ile Val Gln Ala Leu 
                85                  90                  95      


Ala Ala Gly Tyr Asp Phe Asp Thr Asp Phe Ala Gly Val Asp Lys Asp 
            100                 105                 110         


Gly Cys Val Thr Gly Val Thr Arg Gly Asp Ile Leu Ala Gln Glu Asp 
        115                 120                 125             


Leu Lys Val Val Ile Ile Lys Ala Asn Thr Asp Val Lys Ile Asp Gly 
    130                 135                 140                 


Lys Ile Leu Tyr Val Ser Cys Asp Asn Val Thr Val Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Lys Glu Gly Thr Gly Ile Glu Lys Thr Trp Ile Thr 
                165                 170                 175     


Glu Ala Glu Glu Val Pro Ser Thr Glu Ala Val Leu Glu Thr Glu Ser 
            180                 185                 190         


Thr Glu Asp Ala Gly Asp Val Ile Glu Gly Glu Val Ile Ile Gly Thr 
        195                 200                 205             


Glu Glu Ala Ser Gly Asn Asp Val Val Thr Asn Leu Ser Gly Gly Ser 
    210                 215                 220                 


Ser Gly Thr Asp Val Tyr Thr Tyr Ile Ile Tyr Lys 
225                 230                 235     


<210>  24
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of SG-11 with start methionine

<400>  24

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys 
1               5                   10              


<210>  25
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of SG-11

<400>  25

Gly Val Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr 
1               5                   10                  15      


Asp Glu Thr Glu Leu Lys 
            20          


<210>  26
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of SG-11

<400>  26

Thr Pro Glu Asp Tyr Thr Ala Phe Asn Gly Ile Glu Leu Tyr Gln Gly 
1               5                   10                  15      


Lys 
    


<210>  27
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of SG-11

<400>  27

Ala Asn Thr Asp Val Lys 
1               5       


<210>  28
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of SG-11

<400>  28

Val Asp Gly Glu Ile Cys Tyr Val Ser Cys Gln Asn Val Lys 
1               5                   10                  


<210>  29
<211>  67
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of SG-11

<400>  29

Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr 
1               5                   10                  15      


Gly Gln Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met 
            20                  25                  30          


Glu Met Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu 
        35                  40                  45              


Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile 
    50                  55                  60                  


Val Tyr Lys 
65          


<210>  30
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of SG-11

<400>  30

Asn Ala Val Lys 
1               


<210>  31
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment of SG-11

<400>  31

Thr Pro Glu Asp Tyr Thr Ala Phe Ser Gly Ile Glu Leu Tyr Gln Gly 
1               5                   10                  15      


Lys 
    


<210>  32
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG tag

<400>  32

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  33
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-11


<220>
<221>  misc_feature
<222>  (53)..(53)
<223>  X is any amino acid as defined in the specification

<220>
<221>  misc_feature
<222>  (83)..(84)
<223>  X is any amino acid as defined in the specification

<220>
<221>  misc_feature
<222>  (147)..(147)
<223>  X is any amino acid as defined in the specification

<220>
<221>  misc_feature
<222>  (151)..(151)
<223>  X is any amino acid as defined in the specification

<400>  33

Met Leu Glu Gly Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val 
1               5                   10                  15      


Ile Ala Ser Leu Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu 
            20                  25                  30          


Thr Glu Leu Lys Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala 
        35                  40                  45              


Glu His Gly Lys Xaa Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp 
    50                  55                  60                  


Gly Val Ala Lys Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr 
65                  70                  75                  80  


Ala Phe Xaa Xaa Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu 
                85                  90                  95      


Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly 
            100                 105                 110         


Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp 
        115                 120                 125             


Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly 
    130                 135                 140                 


Glu Ile Xaa Tyr Val Ser Xaa Gln Asn Val Lys Leu Thr Gly Lys Asp 
145                 150                 155                 160 


Ser Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr 
                165                 170                 175     


Ala Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu 
            180                 185                 190         


Ser Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp 
        195                 200                 205             


Asp Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr 
    210                 215                 220                 


Asp Val Tyr Thr Phe Ile Val Tyr Lys 
225                 230             


<210>  34
<211>  161
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SG-21

<400>  34

Tyr Lys Thr Pro Glu Asp Tyr Thr Ala Phe Asn Gly Ile Glu Leu Tyr 
1               5                   10                  15      


Gln Gly Lys Val Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp Gly 
            20                  25                  30          


Glu Phe Ala Arg Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr Lys 
        35                  40                  45              


Gln Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg Ala 
    50                  55                  60                  


Asn Thr Asp Val Lys Val Asp Gly Glu Ile Cys Tyr Val Ser Cys Gln 
65                  70                  75                  80  


Asn Val Lys Leu Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly Tyr 
                85                  90                  95      


Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly Gln 
            100                 105                 110         


Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu Met 
        115                 120                 125             


Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala Ala 
    130                 135                 140                 


Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile Val Tyr 
145                 150                 155                 160 


Lys 
    


<210>  35
<211>  483
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG-21

<400>  35
tacaagacac cggaggatta taccgcattt aatggaattg aactctatca ggggaaagtc       60

gttgcttccc tggcggcagg atacgtctac gacggggagt tcgcccgcgt ggaggaaggc      120

aaggttgtgg gagctgccac aaaacaggat atttactctg aggatgattt gaaagttgcc      180

atcatccgtg ccaatacgga tgtgaaggtg gacggtgaga tctgctatgt ctcctgtcag      240

aatgtgaagc tgaccggaaa agacagtgtg tcgatccgtg acggatatta tcttgagacg      300

ggaagcgtga cggcatccgt ggatgtgacc ggacaggaga gcgtcgggac cgagcagctt      360

tcgggaaccg aacagatgga gatgaccggg gagccggtga atgcggatga taccgagcag      420

acagaggcgg cggccggtga cggttcgttc gagacagacg tatatacttt cattgtctac      480

aaa                                                                    483


<210>  36
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SG-21 with start methionine

<400>  36

Met Tyr Lys Thr Pro Glu Asp Tyr Thr Ala Phe Asn Gly Ile Glu Leu 
1               5                   10                  15      


Tyr Gln Gly Lys Val Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp 
            20                  25                  30          


Gly Glu Phe Ala Arg Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr 
        35                  40                  45              


Lys Gln Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg 
    50                  55                  60                  


Ala Asn Thr Asp Val Lys Val Asp Gly Glu Ile Cys Tyr Val Ser Cys 
65                  70                  75                  80  


Gln Asn Val Lys Leu Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly 
                85                  90                  95      


Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly 
            100                 105                 110         


Gln Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu 
        115                 120                 125             


Met Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala 
    130                 135                 140                 


Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile Val 
145                 150                 155                 160 


Tyr Lys 
        


<210>  37
<211>  486
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG-21 with start methionine

<400>  37
atgtacaaga caccggagga ttataccgca tttaatggaa ttgaactcta tcaggggaaa       60

gtcgttgctt ccctggcggc aggatacgtc tacgacgggg agttcgcccg cgtggaggaa      120

ggcaaggttg tgggagctgc cacaaaacag gatatttact ctgaggatga tttgaaagtt      180

gccatcatcc gtgccaatac ggatgtgaag gtggacggtg agatctgcta tgtctcctgt      240

cagaatgtga agctgaccgg aaaagacagt gtgtcgatcc gtgacggata ttatcttgag      300

acgggaagcg tgacggcatc cgtggatgtg accggacagg agagcgtcgg gaccgagcag      360

ctttcgggaa ccgaacagat ggagatgacc ggggagccgg tgaatgcgga tgataccgag      420

cagacagagg cggcggccgg tgacggttcg ttcgagacag acgtatatac tttcattgtc      480

tacaaa                                                                 486


<210>  38
<211>  161
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-21

<400>  38

Tyr Lys Thr Pro Glu Asp Tyr Thr Ala Phe Asn Gly Ile Glu Leu Tyr 
1               5                   10                  15      


Gln Gly Lys Val Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp Gly 
            20                  25                  30          


Glu Phe Ala Arg Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr Lys 
        35                  40                  45              


Gln Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg Ala 
    50                  55                  60                  


Asn Thr Asp Val Lys Val Asp Gly Glu Ile Val Tyr Val Ser Ser Gln 
65                  70                  75                  80  


Asn Val Lys Leu Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly Tyr 
                85                  90                  95      


Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly Gln 
            100                 105                 110         


Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu Met 
        115                 120                 125             


Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala Ala 
    130                 135                 140                 


Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile Val Tyr 
145                 150                 155                 160 


Lys 
    


<210>  39
<211>  161
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-21

<400>  39

Tyr Lys Thr Pro Glu Asp Tyr Thr Ala Phe Asn Asp Ile Glu Leu Tyr 
1               5                   10                  15      


Gln Gly Lys Val Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp Gly 
            20                  25                  30          


Glu Phe Ala Arg Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr Lys 
        35                  40                  45              


Gln Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg Ala 
    50                  55                  60                  


Asn Thr Asp Val Lys Val Asp Gly Glu Ile Val Tyr Val Ser Ser Gln 
65                  70                  75                  80  


Asn Val Lys Leu Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly Tyr 
                85                  90                  95      


Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly Gln 
            100                 105                 110         


Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu Met 
        115                 120                 125             


Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala Ala 
    130                 135                 140                 


Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile Val Tyr 
145                 150                 155                 160 


Lys 
    


<210>  40
<211>  161
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-21

<400>  40

Tyr Lys Thr Pro Glu Asp Tyr Thr Ala Phe Ser Gly Ile Glu Leu Tyr 
1               5                   10                  15      


Gln Gly Lys Val Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp Gly 
            20                  25                  30          


Glu Phe Ala Arg Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr Lys 
        35                  40                  45              


Gln Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg Ala 
    50                  55                  60                  


Asn Thr Asp Val Lys Val Asp Gly Glu Ile Val Tyr Val Ser Ser Gln 
65                  70                  75                  80  


Asn Val Lys Leu Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly Tyr 
                85                  90                  95      


Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly Gln 
            100                 105                 110         


Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu Met 
        115                 120                 125             


Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala Ala 
    130                 135                 140                 


Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile Val Tyr 
145                 150                 155                 160 


Lys 
    


<210>  41
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-21

<400>  41
ttggagggtg aagagtctgt tgtctatgtg ggtaagaaag gtgtgatcgc gtccctggac       60

gtcgagactc tggaccagtc ttactatgat gaaaccgagc tgaagtcgta tgtggacgcc      120

gaagttgagg attacacggc cgagcacggc aaatccgccg tcaaagttga gagcttgaaa      180

gttgaggacg gcgtggcaaa gctgaagatg aaatacaaga ccccagagga ctacacggcg      240

ttcagcggta tcgagctgta tcagggcaaa gtcgtcgcat ccctggcagc gggctatgtg      300

tacgacggtg agtttgcgcg cgtcgaagaa ggcaaagttg tgggtgcggc tacgaaacaa      360

gatatctaca gcgaagatga cctgaaagtc gcgattattc gtgctaacac cgatgttaaa      420

gttgatggcg agattgtgta cgttagcagc caaaacgtaa agctgacggg taaagatagc      480

gtgagcattc gtgatggcta ttatctggaa accggtagcg ttacggcgag cgtcgatgtt      540

accggtcaag agagcgtggg taccgaacag ctgagcggca ccgaacagat ggaaatgacc      600

ggtgaaccgg ttaacgcaga cgacacggaa caaaccgaag ccgcggcagg cgacggtagc      660

ttcgagactg acgtgtacac ctttatcgtg tacaag                                696


<210>  42
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-21

<400>  42

Met Tyr Lys Thr Pro Glu Asp Tyr Thr Ala Phe Ser Gly Ile Glu Leu 
1               5                   10                  15      


Tyr Gln Gly Lys Val Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp 
            20                  25                  30          


Gly Glu Phe Ala Arg Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr 
        35                  40                  45              


Lys Gln Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg 
    50                  55                  60                  


Ala Asn Thr Asp Val Lys Val Asp Gly Glu Ile Val Tyr Val Ser Ser 
65                  70                  75                  80  


Gln Asn Val Lys Leu Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly 
                85                  90                  95      


Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly 
            100                 105                 110         


Gln Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu 
        115                 120                 125             


Met Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala 
    130                 135                 140                 


Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile Val 
145                 150                 155                 160 


Tyr Lys 
        


<210>  43
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-21

<400>  43
atgttggagg gtgaagagtc tgttgtctat gtgggtaaga aaggtgtgat cgcgtccctg       60

gacgtcgaga ctctggacca gtcttactat gatgaaaccg agctgaagtc gtatgtggac      120

gccgaagttg aggattacac ggccgagcac ggcaaatccg ccgtcaaagt tgagagcttg      180

aaagttgagg acggcgtggc aaagctgaag atgaaataca agaccccaga ggactacacg      240

gcgttcagcg gtatcgagct gtatcagggc aaagtcgtcg catccctggc agcgggctat      300

gtgtacgacg gtgagtttgc gcgcgtcgaa gaaggcaaag ttgtgggtgc ggctacgaaa      360

caagatatct acagcgaaga tgacctgaaa gtcgcgatta ttcgtgctaa caccgatgtt      420

aaagttgatg gcgagattgt gtacgttagc agccaaaacg taaagctgac gggtaaagat      480

agcgtgagca ttcgtgatgg ctattatctg gaaaccggta gcgttacggc gagcgtcgat      540

gttaccggtc aagagagcgt gggtaccgaa cagctgagcg gcaccgaaca gatggaaatg      600

accggtgaac cggttaacgc agacgacacg gaacaaaccg aagccgcggc aggcgacggt      660

agcttcgaga ctgacgtgta cacctttatc gtgtacaag                             699


<210>  44
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SG-21 with N-terminal addition

<400>  44

Gln Gln Met Gly Arg Gly Ser Tyr Lys Thr Pro Glu Asp Tyr Thr Ala 
1               5                   10                  15      


Phe Asn Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu Ala 
            20                  25                  30          


Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly Lys 
        35                  40                  45              


Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp Leu 
    50                  55                  60                  


Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly Glu 
65                  70                  75                  80  


Ile Cys Tyr Val Ser Cys Gln Asn Val Lys Leu Thr Gly Lys Asp Ser 
                85                  90                  95      


Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala 
            100                 105                 110         


Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu Ser 
        115                 120                 125             


Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp Asp 
    130                 135                 140                 


Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp 
145                 150                 155                 160 


Val Tyr Thr Phe Ile Val Tyr Lys 
                165             


<210>  45
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SG-21 with N-terminal addition

<400>  45

Gln Gln Met Gly Arg Gly Ser Tyr Lys Thr Pro Glu Asp Tyr Thr Ala 
1               5                   10                  15      


Phe Ser Gly Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu Ala 
            20                  25                  30          


Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly Lys 
        35                  40                  45              


Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp Leu 
    50                  55                  60                  


Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly Glu 
65                  70                  75                  80  


Ile Val Tyr Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp Ser 
                85                  90                  95      


Val Ser Ile Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala 
            100                 105                 110         


Ser Val Asp Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu Ser 
        115                 120                 125             


Gly Thr Glu Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp Asp 
    130                 135                 140                 


Thr Glu Gln Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp 
145                 150                 155                 160 


Val Tyr Thr Phe Ile Val Tyr Lys 
                165             


<210>  46
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal peptide of SG-11

<400>  46

Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly 
1               5                   10                  15      


Gln Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu 
            20                  25                  30          


Met Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala 
        35                  40                  45              


Ala Ala Gly Asp 
    50          


<210>  47
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal peptide of SG-11

<400>  47

Thr Pro Glu Asp Tyr Thr Ala Phe Asn Gly Ile Glu Leu Tyr Gln Gly 
1               5                   10                  15      


Lys Val Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe 
            20                  25                  30          


Ala Arg Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr Lys 
        35                  40                  45      


<210>  48
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal peptide of SG-11

<400>  48

Gln Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg Ala 
1               5                   10                  15      


Asn Thr Asp Val Lys Val Asp Gly Glu Ile Cys Tyr Val Ser Cys Gln 
            20                  25                  30          


Asn Val Lys Leu Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly 
        35                  40                  45          


<210>  49
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal peptide of SG-11

<400>  49

Leu Ala Ala Gly Tyr Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu 
1               5                   10                  15      


Gly Lys Val Val Gly Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp 
            20                  25                  30          


Asp Leu Lys Val Ala Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp 
        35                  40                  45              


Gly Glu 
    50  


<210>  50
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial variant of SG-21


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  X is optional and/or is any amino acid as defined in the 
       specification

<220>
<221>  misc_feature
<222>  (12)..(13)
<223>  X is any amino acid as defined in the specification

<220>
<221>  misc_feature
<222>  (76)..(76)
<223>  X is any amino acid as defined in the specification

<220>
<221>  misc_feature
<222>  (80)..(80)
<223>  X is any amino acid as defined in the specification

<400>  50

Xaa Tyr Lys Thr Pro Glu Asp Tyr Thr Ala Phe Xaa Xaa Ile Glu Leu 
1               5                   10                  15      


Tyr Gln Gly Lys Val Val Ala Ser Leu Ala Ala Gly Tyr Val Tyr Asp 
            20                  25                  30          


Gly Glu Phe Ala Arg Val Glu Glu Gly Lys Val Val Gly Ala Ala Thr 
        35                  40                  45              


Lys Gln Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val Ala Ile Ile Arg 
    50                  55                  60                  


Ala Asn Thr Asp Val Lys Val Asp Gly Glu Ile Xaa Tyr Val Ser Xaa 
65                  70                  75                  80  


Gln Asn Val Lys Leu Thr Gly Lys Asp Ser Val Ser Ile Arg Asp Gly 
                85                  90                  95      


Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp Val Thr Gly 
            100                 105                 110         


Gln Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu Gln Met Glu 
        115                 120                 125             


Met Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln Thr Glu Ala 
    130                 135                 140                 


Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr Phe Ile Val 
145                 150                 155                 160 


Tyr Lys 
        


<210>  51
<211>  3260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNZ8124 full construct

<400>  51
agatctagtc ttataactat actgacaata gaaacattaa caaatctaaa acagtcttaa       60

ttctatcttg agaaagtatt ggtaataata ttattgtcga taacgcgagc ataataaacg      120

gctctgatta aattctgaag tttgttagat acaatgattt cgttcgaagg aactacaaaa      180

taaattataa ggaggcactc aaaatgaaaa aaaagattat ctcagctatt ttaatgtcta      240

cagtgatctt aagtgctgca gccccgttgt caggtgttta cgctgatatc acggccatgg      300

gtactgcagg catgcggtac cactagttct agagagctca agctttcttt gaaccaaaat      360

tagaaaacca aggcttgaaa cgttcaattg aaatggcaat taaacaaatt acagcacgtg      420

ttgctttgat tgatagccaa aaagcagcag ttgataaagc aattactgat attgctgaaa      480

aattgtaatt tataaataaa aatcaccttt tagaggtggt ttttttattt ataaattatt      540

cgtttgattt cgctttcgat agaacaatca aatcgtttct gagacgtttt agcgtttatt      600

tcgtttagtt atcggcataa tcgttaaaac aggcgttatc gtagcgtaaa agcccttgag      660

cgtagcgtgg ctttgcagcg aagatgttgt ctgttagatt atgaaagccg atgactgaat      720

gaaataataa gcgcagcgtc cttctatttc ggttggagga ggctcaaggg agtttgaggg      780

aatgaaattc cctcatgggt ttgattttaa aaattgcttg caattttgcc gagcggtagc      840

gctggaaaat ttttgaaaaa aatttggaat ttggaaaaaa atggggggaa aggaagcgaa      900

ttttgcttcc gtactacgac cccccattaa gtgccgagtg ccaatttttg tgccaaaaac      960

gctctatccc aactggctca agggtttgag gggtttttca atcgccaacg aatcgccaac     1020

gttttcgcca acgtttttta taaatctata tttaagtagc tttatttttg tttttatgat     1080

tacaaagtga tacactaatt ttataaaatt atttgattgg agttttttaa atggtgattt     1140

cagaatcgaa aaaaagagtt atgatttctc tgacaaaaga gcaagataaa aaattaacag     1200

atatggcgaa acaaaaagat ttttcaaaat ctgcggttgc ggcgttagct atagaagaat     1260

atgcaagaaa ggaatcagaa caaaaaaaat aagcgaaagc tcgcgttttt agaaggatac     1320

gagttttcgc tacttgtttt tgataaggta attatatcat ggctattaaa aatactaaag     1380

ctagaaattt tggattttta ttatatcctg actcaattcc taatgattgg aaagaaaaat     1440

tagagagttt gggcgtatct atggctgtca gtcctttaca cgatatggac gaaaaaaaag     1500

ataaagatac atggaatagt agtgatgtta tacgaaatgg aaagcactat aaaaaaccac     1560

actatcacgt tatatatatt gcacgaaatc ctgtaacaat agaaagcgtt aggaacaaga     1620

ttaagcgaaa attggggaat agttcagttg ctcatgttga gatacttgat tatatcaaag     1680

gttcatatga atatttgact catgaatcaa aggacgctat tgctaagaat aaacatatat     1740

acgacaaaaa agatattttg aacattaatg attttgatat tgaccgctat ataacacttg     1800

atgaaagcca aaaaagagaa ttgaagaatt tacttttaga tatagtggat gactataatt     1860

tggtaaatac aaaagattta atggctttta ttcgccttag gggagcggag tttggaattt     1920

taaatacgaa tgatgtaaaa gatattgttt caacaaactc tagcgccttt agattatggt     1980

ttgagggcaa ttatcagtgt ggatatagag caagttatgc aaaggttctt gatgctgaaa     2040

cgggggaaat aaaatgacaa acaaagaaaa agagttattt gctgaaaatg aggaattaaa     2100

aaaagaaatt aaggacttaa aagagcgtat tgaaagatac agagaaatgg aagttgaatt     2160

aagtacaaca atagatttat tgagaggagg gattattgaa taaataaaag cccccctgac     2220

gaaagtcgac ggcaatagtt acccttatta tcaagataag aaagaaaagg atttttcgct     2280

acgctcaaat cctttaaaaa aacacaaaag accacatttt ttaatgtggt cttttattct     2340

tcaactaaag cacccattag ttcaacaaac gaaaattgga taaagtggga tatttttaaa     2400

atatatattt atgttacagt aatattgact tttaaaaaag gattgattct aatgaagaaa     2460

gcagacaagt aagcctccta aattcacttt agataaaaat ttaggaggca tatcaaatga     2520

actttaataa aattgattta gacaattgga agagaaaaga gatatttaat cattatttga     2580

accaacaaac gacttttagt ataaccacag aaattgatat tagtgtttta taccgaaaca     2640

taaaacaaga aggatataaa ttttaccctg catttatttt cttagtgaca agggtgataa     2700

actcaaatac agcttttaga actggttaca atagcgacgg agagttaggt tattgggata     2760

agttagagcc actttataca atttttgatg gtgtatctaa aacattctct ggtatttgga     2820

ctcctgtaaa gaatgacttc aaagagtttt atgatttata cctttctgat gtagagaaat     2880

ataatggttc ggggaaattg tttcccaaaa cacctatacc tgaaaatgct ttttctcttt     2940

ctattattcc ttggacttca tttactgggt ttaacttaaa tatcaataat aatagtaatt     3000

accttctacc cattattaca gcaggaaaat tcattaataa aggtaattca atatatttac     3060

cgctatcttt acaggtacat cattctgttt gtgatggtta tcatgctgga ttgtttatga     3120

actctattca ggaattgtca gataggccta atgactggct tttataatat gagataatgc     3180

cgactgtact ttttacagtc ggttttctaa tgtcactaac ctgccccgtt agttgaagaa     3240

ggtttttata ttacagctcc                                                 3260


<210>  52
<211>  197
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nisA promoter

<400>  52
ctagtcttat aactatactg acaatagaaa cattaacaaa tctaaaacag tcttaattct       60

atcttgagaa agtattggta ataatattat tgtcgataac gcgagcataa taaacggctc      120

tgattaaatt ctgaagtttg ttagatacaa tgatttcgtt cgaaggaact acaaaataaa      180

ttataaggag gcactca                                                     197


<210>  53
<211>  1031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PnisA:SPusp45:SG-11V5:Flag operon

<400>  53
ctagtcttat aactatactg acaatagaaa cattaacaaa tctaaaacag tcttaattct       60

atcttgagaa agtattggta ataatattat tgtcgataac gcgagcataa taaacggctc      120

tgattaaatt ctgaagtttg ttagatacaa tgatttcgtt cgaaggaact acaaaataaa      180

ttataaggag gcactcaaaa tgaaaaaaaa gattatctca gctattttaa tgtctacagt      240

gatcttaagt gctgcagccc cgttgtcagg tgtttacgct gatatcttgg agggtgaaga      300

gtctgttgtc tatgtgggta agaaaggtgt gatcgcgtcc ctggacgtcg agactctgga      360

ccagtcttac tatgatgaaa ccgagctgaa gtcgtatgtg gacgccgaag ttgaggatta      420

cacggccgag cacggcaaat ccgccgtcaa agttgagagc ttgaaagttg aggacggcgt      480

ggcaaagctg aagatgaaat acaagacccc agaggactac acggcgttca gcggtatcga      540

gctgtatcag ggcaaagtcg tcgcatccct ggcagcgggc tatgtgtacg acggtgagtt      600

tgcgcgcgtc gaagaaggca aagttgtggg tgcggctacg aaacaagata tctacagcga      660

agatgacctg aaagtcgcga ttattcgtgc taacaccgat gttaaagttg atggcgagat      720

tgtgtacgtt agcagccaaa acgtaaagct gacgggtaaa gatagcgtga gcattcgtga      780

tggctattat ctggaaaccg gtagcgttac ggcgagcgtc gatgttaccg gtcaagagag      840

cgtgggtacc gaacagctga gcggcaccga acagatggaa atgaccggtg aaccggttaa      900

cgcagacgac acggaacaaa ccgaagccgc ggcaggcgac ggtagcttcg agactgacgt      960

gtacaccttt atcgtgtaca aggactacaa agacgatgac gacaagtaat ctagagagct     1020

caagcttttg a                                                          1031


<210>  54
<211>  829
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPusp45:SG-11V5:Flag without TGA stop codon operon

<400>  54
atgaaaaaaa agattatctc agctatttta atgtctacag tgatcttaag tgctgcagcc       60

ccgttgtcag gtgtttacgc tgatatcttg gagggtgaag agtctgttgt ctatgtgggt      120

aagaaaggtg tgatcgcgtc cctggacgtc gagactctgg accagtctta ctatgatgaa      180

accgagctga agtcgtatgt ggacgccgaa gttgaggatt acacggccga gcacggcaaa      240

tccgccgtca aagttgagag cttgaaagtt gaggacggcg tggcaaagct gaagatgaaa      300

tacaagaccc cagaggacta cacggcgttc agcggtatcg agctgtatca gggcaaagtc      360

gtcgcatccc tggcagcggg ctatgtgtac gacggtgagt ttgcgcgcgt cgaagaaggc      420

aaagttgtgg gtgcggctac gaaacaagat atctacagcg aagatgacct gaaagtcgcg      480

attattcgtg ctaacaccga tgttaaagtt gatggcgaga ttgtgtacgt tagcagccaa      540

aacgtaaagc tgacgggtaa agatagcgtg agcattcgtg atggctatta tctggaaacc      600

ggtagcgtta cggcgagcgt cgatgttacc ggtcaagaga gcgtgggtac cgaacagctg      660

agcggcaccg aacagatgga aatgaccggt gaaccggtta acgcagacga cacggaacaa      720

accgaagccg cggcaggcga cggtagcttc gagactgacg tgtacacctt tatcgtgtac      780

aaggactaca aagacgatga cgacaagtaa tctagagagc tcaagcttt                  829


<210>  55
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPusp45-SG-11V5-Flag fusion protein

<400>  55

Met Lys Lys Lys Ile Ile Ser Ala Ile Leu Met Ser Thr Val Ile Leu 
1               5                   10                  15      


Ser Ala Ala Ala Pro Leu Ser Gly Val Tyr Ala Asp Ile Leu Glu Gly 
            20                  25                  30          


Glu Glu Ser Val Val Tyr Val Gly Lys Lys Gly Val Ile Ala Ser Leu 
        35                  40                  45              


Asp Val Glu Thr Leu Asp Gln Ser Tyr Tyr Asp Glu Thr Glu Leu Lys 
    50                  55                  60                  


Ser Tyr Val Asp Ala Glu Val Glu Asp Tyr Thr Ala Glu His Gly Lys 
65                  70                  75                  80  


Ser Ala Val Lys Val Glu Ser Leu Lys Val Glu Asp Gly Val Ala Lys 
                85                  90                  95      


Leu Lys Met Lys Tyr Lys Thr Pro Glu Asp Tyr Thr Ala Phe Ser Gly 
            100                 105                 110         


Ile Glu Leu Tyr Gln Gly Lys Val Val Ala Ser Leu Ala Ala Gly Tyr 
        115                 120                 125             


Val Tyr Asp Gly Glu Phe Ala Arg Val Glu Glu Gly Lys Val Val Gly 
    130                 135                 140                 


Ala Ala Thr Lys Gln Asp Ile Tyr Ser Glu Asp Asp Leu Lys Val Ala 
145                 150                 155                 160 


Ile Ile Arg Ala Asn Thr Asp Val Lys Val Asp Gly Glu Ile Val Tyr 
                165                 170                 175     


Val Ser Ser Gln Asn Val Lys Leu Thr Gly Lys Asp Ser Val Ser Ile 
            180                 185                 190         


Arg Asp Gly Tyr Tyr Leu Glu Thr Gly Ser Val Thr Ala Ser Val Asp 
        195                 200                 205             


Val Thr Gly Gln Glu Ser Val Gly Thr Glu Gln Leu Ser Gly Thr Glu 
    210                 215                 220                 


Gln Met Glu Met Thr Gly Glu Pro Val Asn Ala Asp Asp Thr Glu Gln 
225                 230                 235                 240 


Thr Glu Ala Ala Ala Gly Asp Gly Ser Phe Glu Thr Asp Val Tyr Thr 
                245                 250                 255     


Phe Ile Val Tyr Lys Asp Tyr Lys Asp Asp Asp Asp Lys 
            260                 265                 


<210>  56
<211>  2421
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PnisA:otsBA operon

<400>  56
ctagtcttat aactatactg acaatagaaa cattaacaaa tctaaaacag tcttaattct       60

atcttgagaa agtattggta ataatattat tgtcgataac gcgagcataa taaacggctc      120

tgattaaatt ctgaagtttg ttagatacaa tgatttcgtt cgaaggaact acaaaataaa      180

ttataaggag gcactcaaaa tgacagaacc gttaaccgaa acccctgaac tatccgcgaa      240

atatgcctgg ttttttgatc ttgatggaac gctggcggaa atcaaaccgc atcccgatca      300

ggtcgtcgtg cctgacaata ttctgcaagg actacagcta ctggcaaccg caagtgatgg      360

tgcattggca ttgatatcag ggcgctcaat ggtggagctt gacgcactgg caaaacctta      420

tcgcttcccg ttagcgggcg tgcatggggc ggagcgccgt gacatcaatg gtaaaacaca      480

tatcgttcat ctgccggatg cgattgcgcg tgatattagc gtgcaactgc atacagtcat      540

cgctcagtat cccggcgcgg agctggaggc gaaagggatg gcttttgcgc tgcattatcg      600

tcaggctccg cagcatgaag acgcattaat gacattagcg caacgtatta ctcagatctg      660

gccacaaatg gcgttacagc agggaaagtg tgttgtcgag atcaaaccga gaggtaccag      720

taaaggtgag gcaattgcag cttttatgca ggaagctccc tttatcgggc gaacgcccgt      780

atttctgggc gatgatttaa ccgatgaatc tggcttcgca gtcgttaacc gactgggcgg      840

aatgtcagta aaaattggca caggtgcaac tcaggcatca tggcgactgg cgggtgtgcc      900

ggatgtctgg agctggcttg aaatgataac caccgcatta caacaaaaaa gagaaaataa      960

caggagtgat gactatgagt cgtttagtcg tagtatctaa ccggattgca ccaccagacg     1020

agcacgccgc cagtgccggt ggccttgccg ttggcatact gggggcactg aaagccgcag     1080

gcggactgtg gtttggctgg agtggtgaaa cagggaatga ggatcagccg ctaaaaaagg     1140

tgaaaaaagg taacattacg tgggcctctt ttaacctcag cgaacaggac cttgacgaat     1200

actacaacca attctccaat gccgttctct ggcccgcttt tcattatcgg ctcgatctgg     1260

tgcaatttca gcgtcctgcc tgggacggct atctacgcgt aaatgcgttg ctggcagata     1320

aattactgcc gctgttgcaa gacgatgaca ttatctggat ccacgattat cacctgttgc     1380

catttgcgca tgaattacgc aaacggggag tgaataatcg cattggtttc tttctgcata     1440

ttcctttccc gacaccggaa atcttcaacg cgctgccgac atatgacacc ttgcttgaac     1500

agctttgtga ttatgatttg ctgggtttcc agacagaaaa cgatcgtctg gcgttcctgg     1560

attgtctttc taacctgacc cgcgtcacga cacgtagcgc aaaaagccat acagcctggg     1620

gcaaagcatt tcgaacagaa gtctacccga tcggcattga accgaaagaa atagccaaac     1680

aggctgccgg gccactgccg ccaaaactgg cgcaacttaa agcggaactg aaaaacgtac     1740

aaaatatctt ttctgtcgaa cggctggatt attccaaagg tttgccagag cgttttctcg     1800

cctatgaagc gttgctggaa aaatatccgc agcatcatgg taaaattcgt tatacccaga     1860

ttgcaccaac gtcgcgtggt gatgtgcaag cctatcagga tattcgtcat cagctcgaaa     1920

atgaagctgg acgaattaat ggtaaatacg ggcaattagg ctggacgccg ctttattatt     1980

tgaatcagca ttttgaccgt aaattactga tgaaaatatt ccgctactct gacgtgggct     2040

tagtgacgcc actgcgtgac gggatgaacc tggtagcaaa agagtatgtt gctgctcagg     2100

acccagccaa tccgggcgtt cttgttcttt cgcaatttgc gggagcggca aacgagttaa     2160

cgtcggcgtt aattgttaac ccctacgatc gtgacgaagt tgcagctgcg ctggatcgtg     2220

cattgactat gtcgctggcg gaacgtattt cccgtcatgc agaaatgctg gacgttatcg     2280

tgaaaaacga tattaaccac tggcaggagt gcttcattag cgacctaaag cagatagttc     2340

cgcgaagcgc ggaaagccag cagcgcgata aagttgctac ctttccaaag cttgcgtaga     2400

tgaaaaaaaa gattatctca g                                               2421


<210>  57
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  otsBAfw oligonucleotide primer

<400>  57
ttataaggag gcactcaaaa tgacagaacc gttaacc                                37


<210>  58
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ostBArw oligonucleotide primer

<400>  58
ctgagataat cttttttttc atctacgcaa gctttggaaa ggta                        44


<210>  59
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNZ8124fw oligonucleotide primer

<400>  59
tttgagtgcc tccttataa                                                    19


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNZ8124rv oligonucleotide primer

<400>  60
atgaaaaaaa agattatctc                                                   20


<210>  61
<211>  897
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pusp45:SPusp45:SG-11V5 operon

<400>  61
gatatctgtt ttgtaatcat aaagaaatat taaggtgggg taggaatagt ataatatgtt       60

tattcaaccg aacttaatgg gaggaaaaat taaaaaagaa cagttatgaa aaaaaagatt      120

atctcagcta ttttaatgtc tacagtgata ctttctgctg cagccccgtt gtcaggtgtt      180

tacgctccat ggttggaggg tgaagagtct gttgtctatg tgggtaagaa aggtgtgatc      240

gcgtccctgg acgtcgagac tctggaccag tcttactatg atgaaaccga gctgaagtcg      300

tatgtggacg ccgaagttga ggattacacg gccgagcacg gcaaatccgc cgtcaaagtt      360

gagagcttga aagttgagga cggcgtggca aagctgaaga tgaaatacaa gaccccagag      420

gactacacgg cgttcagcgg tatcgagctg tatcagggca aagtcgtcgc atccctggca      480

gcgggctatg tgtacgacgg tgagtttgcg cgcgtcgaag aaggcaaagt tgtgggtgcg      540

gctacgaaac aagatatcta cagcgaagat gacctgaaag tcgcgattat tcgtgctaac      600

accgatgtta aagttgatgg cgagattgtg tacgttagca gccaaaacgt aaagctgacg      660

ggtaaagata gcgtgagcat tcgtgatggc tattatctgg aaaccggtag cgttacggcg      720

agcgtcgatg ttaccggtca agagagcgtg ggtaccgaac agctgagcgg caccgaacag      780

atggaaatga ccggtgaacc ggttaacgca gacgacacgg aacaaaccga agccgcggca      840

ggcgacggta gcttcgagac tgacgtgtac acctttatcg tgtacaagtg atctaga         897


<210>  62
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  usp45fw oligonucleotide primer

<400>  62
atcgggatat ctgttttgta atcataaaga aatattaagg t                           41


<210>  63
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  usp45rv oligonucleotide primer

<400>  63
atcggccatg gagcgtaaac acctgacaac ggggctgcag                             40


<210>  64
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG-11V5NcoIfw oligonucleotide primer

<400>  64
atcggccatg gttggagggt gaagagtctg t                                      31


<210>  65
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG-11V5XbaIrv oligonucleotide primer

<400>  65
atcggtctag attacttgta cacgataaag gtgt                                   34


<210>  66
<211>  982
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PnisA:SPusp45:SG-11V5 operon

<400>  66
ctagtcttat aactatactg acaatagaaa cattaacaaa tctaaaacag tcttaattct       60

atcttgagaa agtattggta ataatattat tgtcgataac gcgagcataa taaacggctc      120

tgattaaatt ctgaagtttg ttagatacaa tgatttcgtt cgaaggaact acaaaataaa      180

ttataaggag gcactcaaaa tgaaaaaaaa gattatctca gctattttaa tgtctacagt      240

gatcttaagt gctgcagccc cgttgtcagg tgtttacgct gatatcttgg agggtgaaga      300

gtctgttgtc tatgtgggta agaaaggtgt gatcgcgtcc ctggacgtcg agactctgga      360

ccagtcttac tatgatgaaa ccgagctgaa gtcgtatgtg gacgccgaag ttgaggatta      420

cacggccgag cacggcaaat ccgccgtcaa agttgagagc ttgaaagttg aggacggcgt      480

ggcaaagctg aagatgaaat acaagacccc agaggactac acggcgttca gcggtatcga      540

gctgtatcag ggcaaagtcg tcgcatccct ggcagcgggc tatgtgtacg acggtgagtt      600

tgcgcgcgtc gaagaaggca aagttgtggg tgcggctacg aaacaagata tctacagcga      660

agatgacctg aaagtcgcga ttattcgtgc taacaccgat gttaaagttg atggcgagat      720

tgtgtacgtt agcagccaaa acgtaaagct gacgggtaaa gatagcgtga gcattcgtga      780

tggctattat ctggaaaccg gtagcgttac ggcgagcgtc gatgttaccg gtcaagagag      840

cgtgggtacc gaacagctga gcggcaccga acagatggaa atgaccggtg aaccggttaa      900

cgcagacgac acggaacaaa ccgaagccgc ggcaggcgac ggtagcttcg agactgacgt      960

gtacaccttt atcgtgtaca ag                                               982


<210>  67
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal signal peptide derived from the usp45 protein

<400>  67

Met Lys Lys Lys Ile Ile Ser Ala Ile Leu Met Ser Thr Val Ile Leu 
1               5                   10                  15      


Ser Ala Ala Ala Pro Leu Ser Gly Val Tyr Ala 
            20                  25          


<210>  68
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG11fw oligonucleotide primer

<400>  68
aggtgtttac gctgatatct tggagggtga agagtctgt                              39


<210>  69
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SG11rv oligonucleotide primer

<400>  69
aaagcttgag ctctctagat tacttgtcgt catcgtcttt gtagtccttg tacacgataa       60

aggtgt                                                                  66


<210>  70
<211>  99
<212>  DNA
<213>  Lactococcus lactis

<400>  70
tgttttgtaa tcataaagaa atattaaggt ggggtaggaa tagtataata tgtttattca       60

accgaactta atgggaggaa aaattaaaaa agaacagtt                              99


<210>  71
<211>  152
<212>  DNA
<213>  Lactococcus lactis

<400>  71
tggatatttt ttataaatct ggtttgaaca aattatattg acatctcttt ttctatcctg       60

ataattctga gaggttattt tgggaaatac tattgaacca tatcgaggtg gtgtggtata      120

atgaagggaa ttaaaaaaga taggaaaatt tc                                    152


<210>  72
<211>  279
<212>  PRT
<213>  Lactococcus lactis

<400>  72

Met Thr Tyr Ala Asp Lys Ile Phe Lys Gln Asn Ile Gln Asn Ile Leu 
1               5                   10                  15      


Asp Asn Gly Val Phe Ser Glu Asn Ala Arg Pro Lys Tyr Lys Asp Gly 
            20                  25                  30          


Gln Thr Ala Asn Ser Lys Tyr Val Thr Gly Ser Phe Val Thr Tyr Asp 
        35                  40                  45              


Leu Gln Lys Gly Glu Phe Pro Ile Thr Thr Leu Arg Pro Ile Pro Ile 
    50                  55                  60                  


Lys Ser Ala Ile Lys Glu Leu Met Trp Ile Tyr Gln Asp Gln Thr Ser 
65                  70                  75                  80  


Glu Leu Ala Ile Leu Glu Glu Lys Tyr Gly Val Lys Tyr Trp Gly Glu 
                85                  90                  95      


Trp Gly Ile Gly Asp Gly Thr Ile Gly Gln Arg Tyr Gly Ala Thr Val 
            100                 105                 110         


Lys Lys Tyr Asn Ile Ile Gly Lys Leu Leu Asp Gly Leu Ala Lys Asn 
        115                 120                 125             


Pro Trp Asn Arg Arg Asn Ile Ile Asn Leu Trp Gln Tyr Glu Asp Phe 
    130                 135                 140                 


Glu Glu Thr Glu Gly Leu Leu Pro Cys Ala Phe Gln Thr Met Phe Asp 
145                 150                 155                 160 


Val Arg Arg Glu Gln Asp Gly Gln Ile Tyr Leu Asp Ala Thr Leu Ile 
                165                 170                 175     


Gln Arg Ser Asn Asp Met Leu Val Ala His His Ile Asn Ala Met Gln 
            180                 185                 190         


Tyr Val Ala Leu Gln Met Met Ile Ala Lys His Phe Ser Trp Lys Val 
        195                 200                 205             


Gly Lys Phe Phe Tyr Phe Val Asn Asn Leu His Ile Tyr Asp Asn Gln 
    210                 215                 220                 


Phe Glu Gln Ala Asn Glu Leu Val Lys Arg Thr Ala Ser Asp Lys Glu 
225                 230                 235                 240 


Pro Arg Leu Val Leu Asn Val Pro Asp Gly Thr Asn Phe Phe Asp Ile 
                245                 250                 255     


Lys Pro Glu Asp Phe Glu Leu Val Asp Tyr Glu Pro Val Lys Pro Gln 
            260                 265                 270         


Leu Lys Phe Asp Leu Ala Ile 
        275                 


<210>  73
<211>  297
<212>  PRT
<213>  Lactococcus lactis

<400>  73

Met Ser Ala Lys Glu Thr Ile Glu Lys Leu Gln Asn Ala Arg Ile Ile 
1               5                   10                  15      


Thr Ala Leu Val Thr Pro Phe Lys Glu Asn Gly Gln Ile Asn Phe Gly 
            20                  25                  30          


Ala Phe Pro Lys Leu Ile Glu Asp Leu Leu Ala Asn His Thr Glu Gly 
        35                  40                  45              


Leu Ile Leu Ala Gly Thr Thr Ala Glu Ser Pro Thr Leu Thr His Asp 
    50                  55                  60                  


Glu Glu Leu Ala Ile Phe Ala Ala Val Asn Lys Ile Val Asp Gly Arg 
65                  70                  75                  80  


Ile Pro Leu Ile Ala Gly Val Gly Thr Asn Asp Thr Arg Asp Ser Val 
                85                  90                  95      


Glu Phe Val Lys Glu Val Ala Glu Leu Gly Tyr Ile Asp Ala Gly Leu 
            100                 105                 110         


Ala Val Thr Pro Tyr Tyr Asn Lys Pro Ser Gln Glu Gly Ile Tyr Gln 
        115                 120                 125             


His Phe Lys Ala Ile Ala Thr Ala Ser Asp Leu Pro Ile Ile Leu Tyr 
    130                 135                 140                 


Asn Ile Pro Gly Arg Val Val Thr Glu Ile Gln Val Glu Thr Ile Leu 
145                 150                 155                 160 


Arg Leu Ala Glu Leu Glu Asn Val Ile Ala Ile Lys Glu Cys Thr Asn 
                165                 170                 175     


Thr Asp Asn Leu Ala Tyr Leu Ile Glu Lys Leu Pro Lys Asp Phe Leu 
            180                 185                 190         


Val Tyr Thr Gly Glu Asp Gly Leu Ala Phe His Thr Lys Ala Leu Gly 
        195                 200                 205             


Gly Gln Gly Val Ile Ser Val Ala Ser His Ile Leu Gly Gln Glu Phe 
    210                 215                 220                 


Phe Glu Met Phe Ala Glu Ile Asp Gln Gly Ser Ile Gln Lys Ala Ala 
225                 230                 235                 240 


Ala Ile Gln Arg Lys Ile Leu Pro Lys Ile Asn Ala Leu Phe Ser Val 
                245                 250                 255     


Thr Ser Pro Ala Pro Ile Lys Thr Val Leu Asn Ala Lys Gly Tyr Glu 
            260                 265                 270         


Val Gly Gly Leu Arg Leu Pro Leu Val Ala Cys Thr Thr Glu Glu Ser 
        275                 280                 285             


Lys Ile Ile Leu Glu Lys Ile Gly Asn 
    290                 295         


<210>  74
<211>  473
<212>  PRT
<213>  Lactococcus lactis

<400>  74

Met Lys Trp Ser Thr Lys Gln Arg Tyr Arg Thr Tyr Asp Ser Tyr Ser 
1               5                   10                  15      


Glu Ser Asp Leu Glu Ser Leu Arg Lys Leu Ala Leu Lys Ser Pro Trp 
            20                  25                  30          


Lys Ser Asn Phe His Ile Glu Pro Glu Thr Gly Leu Leu Asn Asp Pro 
        35                  40                  45              


Asn Gly Phe Ser Tyr Phe Asn Glu Lys Trp His Leu Phe Tyr Gln His 
    50                  55                  60                  


Phe Pro Phe Gly Pro Val His Gly Leu Lys Ser Trp Val His Leu Val 
65                  70                  75                  80  


Ser Asp Asp Leu Val His Phe Glu Lys Thr Gly Leu Val Leu Tyr Pro 
                85                  90                  95      


Asp Thr Lys Tyr Asp Asn Ala Gly Val Tyr Ser Gly Ser Ala Leu Ala 
            100                 105                 110         


Phe Glu Asn Phe Leu Phe Leu Ile Tyr Thr Gly Asn His Arg Gly Glu 
        115                 120                 125             


Asp Trp Val Arg Thr Pro Tyr Gln Leu Gly Ala Lys Ile Asp Lys Asn 
    130                 135                 140                 


Asn Gln Leu Val Lys Phe Thr Glu Pro Leu Ile Tyr Pro Asp Phe Ser 
145                 150                 155                 160 


Gln Thr Thr Asp His Phe Arg Asp Pro Gln Ile Phe Ser Phe Gln Gly 
                165                 170                 175     


Gln Ile Tyr Cys Leu Ile Gly Ala Gln Ser Ser Gln Lys Asn Gly Ile 
            180                 185                 190         


Ile Lys Leu Tyr Lys Ala Ile Glu Asn Asn Leu Thr Asp Trp Lys Asp 
        195                 200                 205             


Leu Gly Asn Leu Asp Phe Ser Lys Glu Lys Met Gly Tyr Met Ile Glu 
    210                 215                 220                 


Cys Pro Asn Leu Ile Phe Ile Asn Gly Arg Ser Val Leu Val Phe Cys 
225                 230                 235                 240 


Pro Gln Gly Leu Asp Lys Ser Ile Val Lys Tyr Asp Asn Ile Tyr Pro 
                245                 250                 255     


Asn Val Tyr Val Ile Ala Asp Asp Phe Thr Thr Gly Ser Lys Asn Gln 
            260                 265                 270         


Leu Lys Asn Ala Gly Gln Leu Ile Asn Leu Asp Glu Gly Phe Asp Cys 
        275                 280                 285             


Tyr Ala Thr Gln Ser Phe Asn Ala Pro Asp Gly Ser Ala Tyr Ala Ile 
    290                 295                 300                 


Ser Trp Leu Gly Leu Pro Glu Thr Ser Tyr Pro Thr Asp Lys Tyr Asn 
305                 310                 315                 320 


Val Gln Gly Val Leu Ser Met Val Lys Lys Leu Ser Ile Lys Asp Asn 
                325                 330                 335     


Lys Leu Tyr Gln Tyr Pro Val Glu Lys Met Lys Glu Leu Arg Gln Met 
            340                 345                 350         


Glu Gln Asp Leu Leu Leu Ala Asp Asn Asn Ile Ile Thr Ser Asn Ser 
        355                 360                 365             


Tyr Glu Leu Glu Val Asp Phe Arg Gln Gln Thr Ser Thr Leu Leu Ser 
    370                 375                 380                 


Leu Met Thr Asn Glu Lys Gly Asp Ser Ala Leu Lys Val Glu Ile Asp 
385                 390                 395                 400 


Lys Glu Asn Asn Thr Ile Thr Leu Ile Arg Asn Tyr Glu Lys Arg Leu 
                405                 410                 415     


Ala His Val Lys Ile Glu Lys Met Asn Val Phe Ile Asp Gln Ser Ile 
            420                 425                 430         


Phe Glu Ile Phe Ile Asn Asp Gly Glu Lys Val Leu Ser Asp Cys Arg 
        435                 440                 445             


Val Phe Pro Asn Lys Asn Gln Tyr Ser Ile Arg Ser Gln Asn Pro Ile 
    450                 455                 460                 


Lys Ile Lys Leu Trp Glu Leu Lys Lys 
465                 470             


<210>  75
<211>  751
<212>  PRT
<213>  Lactococcus lactis

<400>  75

Met Lys Gln Ile Lys Arg Ile Met Gly Ile Asp Pro Trp Lys Ile Thr 
1               5                   10                  15      


Ser Asn Gln Ile Glu Lys Glu Asp Arg Arg Leu Gln Glu Ser Leu Thr 
            20                  25                  30          


Ser Ile Gly Asn Gly Tyr Met Gly Met Arg Gly Asn Phe Ser Glu Thr 
        35                  40                  45              


Tyr Ser Gly Asp Ser His Gln Gly Thr Tyr Ile Ala Gly Val Trp Phe 
    50                  55                  60                  


Pro Asp Lys Thr Arg Val Gly Trp Trp Lys Asn Gly Tyr Pro Glu Tyr 
65                  70                  75                  80  


Phe Gly Lys Ala Ile Asn Ala Leu Asn Phe Ala Ser Val Arg Val Phe 
                85                  90                  95      


Ile Asp Asp Lys Glu Val Asp Leu Ala Ala Ser His Val Thr Asp Phe 
            100                 105                 110         


Asn Leu Ser Leu Asp Met Gln Lys Gly Val Leu Thr Tyr Thr Tyr Val 
        115                 120                 125             


Ala Tyr Gly Val Arg Val Thr Ala Glu Arg Phe Phe Ser Ile Ala Gln 
    130                 135                 140                 


Gln Glu Leu Ala Val Phe Ala Phe Met Phe Glu Ser Leu Asp Gly Glu 
145                 150                 155                 160 


Ile His Gln Ile Arg Thr Val Ser Val Ile Asp Ala Asn Val Arg Asn 
                165                 170                 175     


Glu Asp Ser Asn Tyr Asp Glu Lys Phe Trp Thr Val Lys Asn Leu Asp 
            180                 185                 190         


Asn Thr Ala Thr Gly Ser Phe Ile Val Thr Glu Thr Ile Pro Asn Pro 
        195                 200                 205             


Phe Gly Val Glu Gln Phe Thr Val Ala Ala Lys Gln Ser Phe Ala Gly 
    210                 215                 220                 


Asp Phe Ala Arg Val Lys Gln Glu Thr Arg Glu Thr Ser Val Leu Asp 
225                 230                 235                 240 


Val Tyr Glu Ala Lys Leu Val Glu Asn Ala Pro Leu Thr Phe Ile Lys 
                245                 250                 255     


Asn Val Leu Val Val Thr Ser Arg Asp Ile Lys Pro Ser Asn Leu Thr 
            260                 265                 270         


Lys Val Leu Ser Asn Leu Thr Leu Glu Ile Ser Lys Lys Thr Tyr Asn 
        275                 280                 285             


Lys Phe Tyr Lys Glu Gln Glu Glu Ala Trp Ala Lys Arg Trp Glu Ile 
    290                 295                 300                 


Ala Asp Val Gln Ile Asp Gly Ser Ala Glu Ala Gln Gln Gly Ile Arg 
305                 310                 315                 320 


Phe Asn Leu Phe Gln Leu Phe Ser Thr Tyr Tyr Gly Glu Asp Glu Arg 
                325                 330                 335     


Leu Asn Ile Gly Pro Lys Gly Phe Thr Gly Glu Lys Tyr Gly Gly Ala 
            340                 345                 350         


Thr Tyr Trp Asp Thr Glu Ala Tyr Ala Val Pro Leu Tyr Leu Ala Leu 
        355                 360                 365             


Ser Asp Glu Lys Val Ala Lys Asn Leu Leu Lys Tyr Arg His Asn Gln 
    370                 375                 380                 


Leu Pro Gln Ala Gln His Asn Ala Arg Gln Gln Gly Leu Lys Gly Ala 
385                 390                 395                 400 


Leu Tyr Pro Met Val Thr Phe Thr Gly Val Glu Cys His Asn Glu Trp 
                405                 410                 415     


Glu Ile Thr Phe Glu Glu Ile His Arg Asn Gly Ala Met Ala Tyr Ala 
            420                 425                 430         


Ile Tyr Asn Tyr Thr Asn Tyr Thr Gly Asp Glu Thr Tyr Leu Ala Gln 
        435                 440                 445             


Glu Gly Leu Glu Val Leu Val Glu Ile Ala Arg Phe Trp Ala Asp Arg 
    450                 455                 460                 


Val His Tyr Ser Gln Arg Asn Asp Lys Tyr Met Ile His Gly Val Thr 
465                 470                 475                 480 


Gly Pro Asn Glu Tyr Glu Asn Asn Ile Asn Asn Asn Trp Tyr Thr Asn 
                485                 490                 495     


Lys Leu Ala Ala Trp Val Leu Thr Tyr Thr Ala Glu Ser Leu Glu Lys 
            500                 505                 510         


Tyr Pro Arg Thr Asp Leu Ile Ser Ser Glu Glu Val Ala His Trp Gly 
        515                 520                 525             


Glu Ile Val Asp Lys Met Tyr Tyr Pro Glu Asp Lys Glu Leu Gly Ile 
    530                 535                 540                 


Phe Val Gln His Asp Gly Tyr Leu Asp Lys Asp Leu Thr Pro Val Ala 
545                 550                 555                 560 


Gln Leu Asp Pro Lys Asn Leu Pro Leu Asn Gln Asn Trp Ser Trp Asp 
                565                 570                 575     


Lys Val Leu Arg Ser Pro Tyr Ile Lys Gln Ala Asp Val Leu Gln Gly 
            580                 585                 590         


Ile Tyr Phe Phe Gly Asn Gln Phe Ser Met Ala Glu Lys Gln Arg Asn 
        595                 600                 605             


Phe Asp Phe Tyr Glu Pro Leu Thr Val His Glu Ser Ser Leu Ser Pro 
    610                 615                 620                 


Ser Ile His Ala Ile Leu Ala Ala Glu Leu Gly Met Glu Asp Lys Ala 
625                 630                 635                 640 


Val Glu Met Tyr Glu Arg Thr Ala Arg Leu Asp Leu Asp Asn Tyr Asn 
                645                 650                 655     


Asn Asp Thr Glu Asp Gly Leu His Ile Thr Ser Met Thr Gly Ser Trp 
            660                 665                 670         


Leu Ala Ile Val His Gly Phe Ala Gln Met Lys Thr Trp Glu Ala Gln 
        675                 680                 685             


Leu Ser Phe Ala Pro Phe Leu Pro Gln Ala Trp Ile Gly Tyr Ala Phe 
    690                 695                 700                 


His Ile Asn Tyr Arg Gly Cys Leu Leu Lys Ile Ser Val Gly Gln Glu 
705                 710                 715                 720 


Val Lys Ile Glu Leu Leu Arg Gly Gln Ala Leu Ser Leu Lys Ile Tyr 
                725                 730                 735     


Asp Glu Thr Val Glu Leu Ser Asp Ser Tyr Ile Thr Lys Thr Arg 
            740                 745                 750     


<210>  76
<211>  998
<212>  PRT
<213>  Lactococcus lactis

<400>  76

Met Ala Met Met Thr Met Ile Asp Val Leu Glu Arg Lys Asp Trp Glu 
1               5                   10                  15      


Asn Pro Val Val Ser Asn Trp Asn Arg Leu Pro Met His Thr Pro Met 
            20                  25                  30          


Asp Leu Leu Glu Lys Gln Ser Leu Asn Gly Leu Trp Asn Phe Asp His 
        35                  40                  45              


Phe Ser Arg Ile Ser Asp Val Pro Lys Asn Trp Leu Glu Leu Thr Glu 
    50                  55                  60                  


Ser Lys Thr Glu Ile Ile Val Pro Ser Asn Trp Gln Ile Glu Phe Lys 
65                  70                  75                  80  


Asp Lys Ser Asp Val Pro Ile Tyr Thr Asn Val Thr Tyr Pro Ile Pro 
                85                  90                  95      


Ile Gln Pro Pro Tyr Val Pro Glu Ala Asn Pro Val Gly Ala Tyr Ser 
            100                 105                 110         


Arg Tyr Phe Asp Ile Thr Lys Glu Trp Leu Glu Ser Gly His Val His 
        115                 120                 125             


Leu Thr Phe Glu Gly Val Gly Ser Ala Phe His Phe Trp Leu Asn Gly 
    130                 135                 140                 


Glu Tyr Gly Gly Tyr Ser Glu Asp Ser Arg Leu Pro Ala Glu Phe Asp 
145                 150                 155                 160 


Ile Ser Asn Leu Ala Lys Glu Gly Gln Asn Cys Leu Lys Val Leu Val 
                165                 170                 175     


Phe Arg Trp Ser Lys Val Thr Tyr Phe Glu Asp Gln Asp Met Trp Arg 
            180                 185                 190         


Met Ser Gly Ile Phe Arg Ser Val Asn Leu Gln Trp Leu Pro Asp Asn 
        195                 200                 205             


Tyr Leu Leu Asp Phe Ser Ile Lys Thr Asp Leu Asp Glu Asp Leu Asp 
    210                 215                 220                 


Phe Ala Asn Val Lys Leu Gln Ala Tyr Ala Lys Asn Ile Asp Asp Ala 
225                 230                 235                 240 


Cys Leu Glu Phe Lys Leu Tyr Asp Asp Glu Gln Leu Ile Gly Glu Cys 
                245                 250                 255     


His Gly Phe Asp Ala Glu Ile Gly Val Val Asn Pro Lys Leu Trp Ser 
            260                 265                 270         


Asp Glu Ile Pro Tyr Leu Tyr Arg Leu Glu Leu Thr Leu Met Asp Arg 
        275                 280                 285             


Ser Gly Ala Val Phe His Lys Glu Thr Lys Lys Ile Gly Ile Arg Lys 
    290                 295                 300                 


Ile Ala Ile Glu Lys Gly Gln Leu Lys Ile Asn Gly Lys Ala Leu Leu 
305                 310                 315                 320 


Val Arg Gly Val Asn Lys His Glu Phe Thr Pro Glu His Gly Tyr Val 
                325                 330                 335     


Val Ser Glu Glu Val Met Ile Lys Asp Ile Lys Leu Met Lys Glu His 
            340                 345                 350         


Asn Phe Asn Ala Val Arg Cys Ser His Tyr Pro Asn Asp Ser Arg Trp 
        355                 360                 365             


Tyr Glu Leu Cys Asp Glu Tyr Gly Leu Tyr Val Met Asp Glu Ala Asn 
    370                 375                 380                 


Ile Glu Thr His Gly Met Thr Pro Met Asn Arg Leu Thr Asn Asp Pro 
385                 390                 395                 400 


Thr Tyr Leu Pro Leu Met Ser Glu Arg Val Thr Arg Met Val Met Arg 
                405                 410                 415     


Asp Arg Asn His Pro Ser Ile Ile Ile Trp Ser Leu Gly Asn Glu Ser 
            420                 425                 430         


Gly Tyr Gly Ser Asn His Gln Ala Leu Tyr Asp Trp Cys Lys Ser Phe 
        435                 440                 445             


Asp Ser Ser Arg Pro Val His Tyr Glu Gly Gly Asp Asp Ala Ser Arg 
    450                 455                 460                 


Gly Ala Thr Asp Ala Thr Asp Ile Ile Cys Pro Met Tyr Ala Arg Val 
465                 470                 475                 480 


Asp Ser Pro Ser Ile Asn Ala Pro Tyr Ser Leu Lys Thr Trp Met Gly 
                485                 490                 495     


Val Ala Gly Glu Asn Arg Pro Leu Ile Leu Cys Glu Tyr Ala His Asp 
            500                 505                 510         


Met Gly Asn Ser Leu Gly Gly Phe Gly Lys Tyr Trp Gln Ala Phe Arg 
        515                 520                 525             


Glu Ile Asp Arg Leu Gln Gly Gly Phe Ile Trp Asp Trp Val Asp Gln 
    530                 535                 540                 


Gly Leu Leu Lys Asp Gly Asn Tyr Ala Tyr Gly Gly Asp Phe Gly Asp 
545                 550                 555                 560 


Lys Pro Asn Asp Arg Gln Phe Ser Leu Asn Gly Leu Val Phe Pro Asn 
                565                 570                 575     


Arg Gln Ala Lys Pro Ala Leu Arg Glu Ala Lys Tyr Trp Gln Gln Tyr 
            580                 585                 590         


Tyr Gln Phe Glu Leu Glu Lys Thr Pro Leu Gly Gln Val Phe Ala Phe 
        595                 600                 605             


Thr Val Thr Asn Glu Tyr Leu Phe Arg Ser Thr Asp Asn Glu Lys Leu 
    610                 615                 620                 


Cys Tyr Gln Leu Ile Asn Gly Leu Glu Val Leu Trp Glu Asn Glu Leu 
625                 630                 635                 640 


Ile Leu Asn Met Pro Ala Gly Gly Ser Met Arg Ile Asp Leu Ser Glu 
                645                 650                 655     


Leu Pro Ile Asp Gly Thr Asp Asn Leu Phe Leu Asn Ile Gln Val Lys 
            660                 665                 670         


Thr Ile Glu Lys Cys Asn Leu Leu Glu Ser Asp Phe Glu Val Ala His 
        675                 680                 685             


Gln Gln Phe Val Leu Gln Glu Lys Ile Asn Phe Thr Asp Arg Ile Asp 
    690                 695                 700                 


Ser Asn Glu Glu Ile Thr Leu Phe Glu Asp Glu Glu Leu Leu Thr Val 
705                 710                 715                 720 


Arg Ser Ala Lys Gln Lys Phe Ile Phe Asn Lys Ser Asn Gly Asn Leu 
                725                 730                 735     


Ser Arg Trp Leu Asp Glu Lys Gly Asn Glu Lys Leu Leu His Glu Leu 
            740                 745                 750         


Ser Glu Gln Phe Thr Arg Ala Pro Leu Asp Asn Asp Ile Gly Val Ser 
        755                 760                 765             


Glu Val Glu His Ile Asp Pro Asn Ala Trp Leu Glu Arg Trp Lys Gly 
    770                 775                 780                 


Ile Gly Phe Tyr Glu Leu Lys Thr Leu Leu Lys Thr Met Ile Ile Gln 
785                 790                 795                 800 


Ala Thr Glu Asn Glu Val Ile Ile Ser Val Gln Thr Asp Tyr Glu Ala 
                805                 810                 815     


Lys Gly Lys Ile Ala Phe Ser Thr Ile Arg Glu Tyr His Ile Phe Arg 
            820                 825                 830         


Asn Gly Glu Leu Leu Leu Lys Val Asp Phe Lys Arg Asn Ile Glu Phe 
        835                 840                 845             


Pro Glu Pro Ala Arg Ile Gly Leu Ser Leu Gln Leu Ala Glu Lys Ala 
    850                 855                 860                 


Glu Asn Val Thr Tyr Phe Gly Leu Gly Pro Asp Glu Asn Tyr Pro Asp 
865                 870                 875                 880 


Arg Arg Gly Ala Ser Leu Phe Gly Gln Trp Asn Leu Arg Ile Thr Asp 
                885                 890                 895     


Met Thr Thr Pro Tyr Ile Phe Pro Ser Glu Asn Gly Leu Arg Met Glu 
            900                 905                 910         


Thr Arg Glu Leu Asn Tyr Asp Arg Leu Lys Val Arg Ala Met Gly Gln 
        915                 920                 925             


Ser Phe Ala Phe Asn Leu Ser Pro Tyr Ser Gln Asn Gln Leu Ala Lys 
    930                 935                 940                 


Lys Gly His Trp His Leu Leu Glu Glu Glu Ala Gly Thr Trp Leu Asn 
945                 950                 955                 960 


Ile Asp Gly Phe His Met Gly Val Gly Gly Asp Asp Ser Trp Ser Pro 
                965                 970                 975     


Ser Val Ala Gln Glu Tyr Leu Leu Thr Lys Gly Asn Tyr His Tyr Glu 
            980                 985                 990         


Val Ser Phe Lys Leu Thr 
        995             


<210>  77
<211>  314
<212>  PRT
<213>  Lactococcus lactis

<400>  77

Met Lys Ser Thr Phe Lys Gln Asp Leu Tyr Tyr Met Phe His Ser Lys 
1               5                   10                  15      


Ile Ile Ile Ile Phe Leu Ser Ile Ser Thr Leu Leu Val Phe Leu Gly 
            20                  25                  30          


Ala Leu Gln Ala Ile Asn Leu Gln Lys Ser Ser Ile Ile Gln Phe Glu 
        35                  40                  45              


Gln Thr Lys Leu Ile Tyr Lys Asn Lys Asn Asp Phe Leu Lys Asp Leu 
    50                  55                  60                  


Asn Lys Asn Tyr Thr Glu Asn Asn Ile Thr Asp Asp Glu Ser Asn Ile 
65                  70                  75                  80  


Asn Thr Glu Val Asn Asn Ser Ala Arg Tyr Ser Tyr Asp Tyr Val Lys 
                85                  90                  95      


Lys Ser Asn Tyr Gln Leu Thr Ser Leu Gly Phe Pro Leu Phe Ile Leu 
            100                 105                 110         


Lys Tyr Leu Gly Leu Ile Phe Leu Pro Ile Val Met Gly Ile Leu Gly 
        115                 120                 125             


Ile Leu Leu Ser Thr Thr Asp Tyr Lys Tyr Gly Thr Tyr Lys Arg Arg 
    130                 135                 140                 


Leu Ser Thr Asn Ser Trp Lys Glu Ile Ile Thr Gly Lys Ile Val Gly 
145                 150                 155                 160 


Leu Ser Ser Val Ile Phe Gly Leu Tyr Phe Tyr Ile Leu Ile Leu Ser 
                165                 170                 175     


Met Ala Val Gly Leu Phe Leu Pro Lys Phe Ser Lys Phe Ile Asp Leu 
            180                 185                 190         


Lys Gln Tyr Asn Ile Asp Ser Pro Asn Pro Ser Leu Phe Ser Ala Phe 
        195                 200                 205             


Thr Leu Val Leu Cys Val Leu Val Leu Ala Leu Ile Thr Gly Ile Leu 
    210                 215                 220                 


Ser Phe Leu Ile Ser Leu Ser Ile Lys Asn Leu Phe Val Ser Leu Ile 
225                 230                 235                 240 


Gly Phe Leu Leu Tyr Tyr Leu Ala Leu Pro Asn Leu Gly Lys Phe Asp 
                245                 250                 255     


Tyr Lys Asn Val Val Met Asn Ile Phe Ser Asn Ala Gly Lys Asp Val 
            260                 265                 270         


Leu Gly Gln Pro Ile Pro Tyr Ile Pro Leu Asp Ile Lys Val Ser Leu 
        275                 280                 285             


Ile Leu Phe Ala Ile Tyr Val Ala Leu Ile Ser Thr Gly Val Leu Ile 
    290                 295                 300                 


Ile Phe Asn Lys Tyr Thr Lys Tyr Ser Met 
305                 310                 


<210>  78
<211>  769
<212>  PRT
<213>  Lactococcus lactis

<400>  78

Met Thr Glu Lys Asp Trp Ile Ile Gln Tyr Asp Lys Lys Glu Val Gly 
1               5                   10                  15      


Lys Arg Ser Tyr Gly Gln Glu Ser Leu Met Ser Leu Gly Asn Gly Tyr 
            20                  25                  30          


Leu Gly Leu Arg Gly Ala Pro Leu Trp Ser Thr Cys Ser Asp Asn His 
        35                  40                  45              


Tyr Pro Gly Leu Tyr Val Ala Gly Val Phe Asn Arg Thr Ser Thr Glu 
    50                  55                  60                  


Val Ala Gly His Asp Val Ile Asn Glu Asp Met Val Asn Trp Pro Asn 
65                  70                  75                  80  


Pro Gln Leu Ile Lys Val Tyr Ile Asp Gly Glu Leu Val Asp Phe Glu 
                85                  90                  95      


Ala Ser Val Glu Lys Gln Ala Thr Ile Asp Phe Lys Asn Ala Leu Gln 
            100                 105                 110         


Ile Glu Arg Tyr Gln Val Lys Leu Ala Lys Gly Asn Leu Thr Leu Val 
        115                 120                 125             


Thr Thr Lys Phe Val Asp Pro Ile Asn Phe His Asp Phe Gly Phe Val 
    130                 135                 140                 


Gly Glu Ile Ile Ala Asp Phe Ser Cys Lys Leu Arg Ile Glu Thr Phe 
145                 150                 155                 160 


Thr Asp Gly Ser Val Leu Asn Gln Asn Val Glu Arg Tyr Arg Ala Phe 
                165                 170                 175     


Asp Ser Lys Glu Phe Glu Val Thr Lys Ile Ser Lys Gly Leu Leu Val 
            180                 185                 190         


Ala Lys Thr Arg Thr Ser Glu Ile Glu Leu Ala Ile Ala Ser Lys Ser 
        195                 200                 205             


Phe Leu Asn Gly Leu Ala Phe Pro Lys Ile Asp Ser Glu Asn Asp Glu 
    210                 215                 220                 


Ile Leu Ala Glu Ala Ile Glu Ile Asp Leu Gln Lys Asn Gln Glu Val 
225                 230                 235                 240 


Gln Phe Asp Lys Thr Ile Val Ile Ala Ser Ser Tyr Glu Ser Lys Asn 
                245                 250                 255     


Pro Val Glu Phe Val Leu Thr Glu Leu Ser Ala Thr Ser Val Ser Lys 
            260                 265                 270         


Ile Gln Glu Asn Asn Thr Asn Tyr Trp Glu Lys Val Trp Ser Asp Ala 
        275                 280                 285             


Asp Ile Val Ile Glu Ser Asp His Glu Asp Leu Gln Arg Met Val Arg 
    290                 295                 300                 


Met Asn Ile Phe His Ile Arg Gln Ala Ala Gln His Gly Ala Asn Gln 
305                 310                 315                 320 


Phe Leu Asp Ala Ser Val Gly Ser Arg Gly Leu Thr Gly Glu Gly Tyr 
                325                 330                 335     


Arg Gly His Ile Phe Trp Asp Glu Ile Phe Val Leu Pro Tyr Tyr Ala 
            340                 345                 350         


Ala Asn Glu Pro Glu Thr Ala Arg Asp Leu Leu Leu Tyr Arg Ile Asn 
        355                 360                 365             


Arg Leu Thr Ala Ala Gln Glu Asn Ala Lys Val Asp Gly Glu Lys Gly 
    370                 375                 380                 


Ala Met Phe Pro Trp Gln Ser Gly Leu Ile Gly Asp Glu Gln Ser Gln 
385                 390                 395                 400 


Phe Val His Leu Asn Thr Val Asn Asn Glu Trp Glu Pro Asp Asn Ser 
                405                 410                 415     


Arg Arg Gln Arg His Val Ser Leu Ala Ile Val Tyr Asn Leu Trp Ile 
            420                 425                 430         


Tyr Ser Gln Leu Thr Glu Asp Glu Ser Ile Leu Thr Asp Gly Gly Leu 
        435                 440                 445             


Asp Leu Ile Ile Glu Thr Thr Lys Phe Trp Leu Asn Lys Ala Glu Leu 
    450                 455                 460                 


Gly Asp Asp Gly Arg Tyr His Ile Asp Gly Val Met Gly Pro Asp Glu 
465                 470                 475                 480 


Tyr His Glu Ala Tyr Pro Gly Gln Glu Gly Gly Ile Cys Asp Asn Ala 
                485                 490                 495     


Tyr Thr Asn Leu Met Leu Thr Trp Gln Leu Asn Trp Leu Thr Glu Leu 
            500                 505                 510         


Ser Glu Lys Gly Phe Glu Ile Pro Lys Glu Leu Leu Glu Lys Ala Gln 
        515                 520                 525             


Lys Val Arg Lys Lys Leu Tyr Leu Asp Ile Asp Glu Asn Gly Val Ile 
    530                 535                 540                 


Ala Gln Tyr Ala Lys Tyr Phe Glu Leu Lys Glu Val Asp Phe Ala Ala 
545                 550                 555                 560 


Tyr Glu Ala Lys Tyr Gly Asp Ile His Arg Ile Asp Arg Leu Met Lys 
                565                 570                 575     


Ala Glu Gly Ile Ser Pro Asp Glu Tyr Gln Val Ala Lys Gln Ala Asp 
            580                 585                 590         


Thr Leu Met Leu Ile Tyr Asn Leu Gly Gln Glu His Val Thr Lys Leu 
        595                 600                 605             


Val Lys Gln Leu Ala Tyr Glu Leu Pro Glu Asn Trp Leu Lys Val Asn 
    610                 615                 620                 


Arg Asp Tyr Tyr Leu Ala Arg Thr Val His Gly Ser Thr Thr Ser Arg 
625                 630                 635                 640 


Pro Val Phe Ala Gly Ile Asp Val Lys Leu Gly Asp Phe Asp Glu Ala 
                645                 650                 655     


Leu Asp Phe Leu Ile Thr Ala Ile Gly Ser Asp Tyr Tyr Asp Ile Gln 
            660                 665                 670         


Gly Gly Thr Thr Ala Glu Gly Val His Ile Gly Val Met Gly Glu Thr 
        675                 680                 685             


Leu Glu Val Ile Gln Asn Glu Phe Ala Gly Leu Ser Leu Arg Glu Gly 
    690                 695                 700                 


Gln Phe Ala Ile Ala Pro Tyr Leu Pro Lys Ser Trp Thr Lys Leu Lys 
705                 710                 715                 720 


Phe Asn Gln Ile Phe Arg Gly Thr Lys Val Glu Ile Leu Ile Glu Asn 
                725                 730                 735     


Gly Gln Leu Leu Leu Thr Ala Ser Ala Asp Leu Leu Thr Lys Val Tyr 
            740                 745                 750         


Asp Asp Glu Val Gln Leu Lys Ala Gly Val Gln Thr Lys Phe Asp Leu 
        755                 760                 765             


Lys 
    


<210>  79
<211>  445
<212>  PRT
<213>  Lactococcus lactis

<400>  79

Met Asn Asn Phe Ile Gln Asn Lys Ile Met Pro Pro Met Met Lys Phe 
1               5                   10                  15      


Leu Asn Thr Arg Ala Val Thr Ala Ile Lys Asn Gly Met Ile Tyr Pro 
            20                  25                  30          


Ile Pro Phe Ile Ile Ile Gly Ser Val Phe Leu Ile Leu Gly Gln Leu 
        35                  40                  45              


Pro Phe Gln Ala Gly Gln Asp Phe Met Asn Lys Ile Lys Leu Gly Pro 
    50                  55                  60                  


Leu Phe Leu Gln Ile Asn Asn Ala Ser Phe Gly Ile Met Ala Leu Leu 
65                  70                  75                  80  


Ala Val Phe Gly Ile Ala Tyr Ala Trp Val Arg Asp Ala Gly Tyr Glu 
                85                  90                  95      


Gly Val Pro Ala Gly Leu Thr Gly Val Ile Val His Ile Leu Leu Gln 
            100                 105                 110         


Pro Asp Thr Ile His Gln Val Thr Ser Val Thr Asp Pro Thr Lys Thr 
        115                 120                 125             


Ser Thr Ala Phe Gln Val Gly Gly Val Ile Asp Arg Ala Trp Leu Gly 
    130                 135                 140                 


Gly Lys Gly Met Val Leu Ser Ile Ile Val Gly Leu Leu Val Gly Trp 
145                 150                 155                 160 


Ile Tyr Thr Gly Phe Met Arg Arg Asn Ile Thr Ile Lys Met Pro Glu 
                165                 170                 175     


Gln Val Pro Glu Asn Val Ala Ala Ser Phe Thr Ser Leu Val Pro Ala 
            180                 185                 190         


Gly Ala Ile Ile Thr Met Ala Gly Val Val His Gly Ile Thr Thr Ile 
        195                 200                 205             


Gly Phe Asn Thr Thr Phe Ile Glu Leu Val Tyr Lys Trp Ile Gln Thr 
    210                 215                 220                 


Pro Leu Gln His Val Thr Asp Gly Pro Val Gly Val Phe Val Ile Ala 
225                 230                 235                 240 


Phe Met Pro Val Phe Ile Trp Trp Phe Gly Val His Gly Ala Thr Ile 
                245                 250                 255     


Ile Gly Gly Ile Met Gly Pro Leu Leu Gln Ala Asn Ser Ala Asp Asn 
            260                 265                 270         


Ala Ala Leu Tyr Lys Ala Gly His Leu Ser Leu Ser Asn Gly Ala His 
        275                 280                 285             


Ile Val Thr Gln Ser Phe Met Asp Gln Tyr Leu Thr Val Thr Gly Ser 
    290                 295                 300                 


Gly Leu Thr Ile Gly Leu Val Ile Phe Leu Leu Val Ser Ala Lys Ser 
305                 310                 315                 320 


Val Gln Gly Lys Thr Leu Gly Arg Met Glu Ile Gly Pro Ala Val Phe 
                325                 330                 335     


Asn Ile Asn Glu Pro Ile Leu Phe Gly Leu Pro Ile Val Leu Asn Pro 
            340                 345                 350         


Ile Leu Ala Ile Pro Phe Ile Leu Ala Pro Leu Ile Ser Gly Ile Leu 
        355                 360                 365             


Thr Tyr Leu Val Ile Tyr Leu Gly Ile Ile Pro Pro Phe Asn Gly Ala 
    370                 375                 380                 


Tyr Val Pro Trp Thr Thr Pro Ala Val Leu Ser Gly Tyr Leu Val Gly 
385                 390                 395                 400 


Gly Trp Gln Gly Met Val Trp Gln Ile Ile Ile Leu Ala Leu Thr Thr 
                405                 410                 415     


Val Leu Tyr Trp Pro Phe Ala Lys Ala Tyr Asp Asn Ile Leu Leu Lys 
            420                 425                 430         


Glu Glu Ala Glu Thr Glu Ala Gly Ile Asn Ala Ala Glu 
        435                 440                 445 


<210>  80
<211>  650
<212>  PRT
<213>  Lactococcus lactis

<400>  80

Met Asn His Lys Gln Val Ala Glu Arg Ile Leu Asn Ala Val Gly Arg 
1               5                   10                  15      


Asp Asn Ile Gln Gly Ala Arg His Cys Ala Thr Arg Leu Arg Leu Val 
            20                  25                  30          


Leu Lys Asp Thr Gly Val Ile Asp Gln Glu Ala Leu Asp Asn Asp Pro 
        35                  40                  45              


Asp Leu Lys Gly Thr Phe Glu Ala Ala Gly Gln Tyr Gln Ile Ile Val 
    50                  55                  60                  


Gly Pro Gly Asp Val Asn Thr Val Tyr Glu Glu Phe Ile Lys Leu Thr 
65                  70                  75                  80  


Gly Ile Ser Glu Ala Ser Thr Ala Asp Leu Lys Glu Ile Ala Gly Ser 
                85                  90                  95      


Gln Lys Lys Gln Asn Pro Val Met Ala Leu Val Lys Leu Leu Ser Asp 
            100                 105                 110         


Ile Phe Val Pro Leu Ile Pro Ala Leu Val Ala Gly Gly Leu Leu Met 
        115                 120                 125             


Ala Leu Asn Asn Ala Leu Thr Ala Glu His Leu Phe Ala Thr Lys Ser 
    130                 135                 140                 


Leu Val Glu Met Phe Pro Met Trp Lys Gly Phe Ala Asp Ile Val Asn 
145                 150                 155                 160 


Thr Met Ser Ala Ala Pro Phe Thr Phe Met Pro Ile Leu Ile Gly Tyr 
                165                 170                 175     


Ser Ala Thr Lys Arg Phe Gly Gly Asn Pro Tyr Leu Gly Ala Val Val 
            180                 185                 190         


Gly Met Ile Met Val Met Pro Gly Leu Ile Asn Gly Tyr Asn Val Ala 
        195                 200                 205             


Glu Ala Ile Ser Asn His Thr Met Thr Tyr Trp Asp Ile Phe Gly Phe 
    210                 215                 220                 


Lys Val Ala Gln Ala Gly Tyr Gln Gly Gln Val Leu Pro Val Ile Gly 
225                 230                 235                 240 


Val Ala Phe Ile Leu Ala Lys Leu Glu Arg Phe Phe His Lys Tyr Leu 
                245                 250                 255     


Asn Asp Ala Ile Asp Phe Thr Phe Thr Pro Leu Leu Ser Val Ile Ile 
            260                 265                 270         


Thr Gly Phe Leu Thr Phe Thr Ile Val Gly Pro Ala Leu Arg Phe Val 
        275                 280                 285             


Ser Asn Gly Leu Thr Asp Gly Leu Val Gly Leu Tyr Asn Thr Leu Gly 
    290                 295                 300                 


Ala Leu Gly Met Leu Val Phe Gly Gly Phe Tyr Ser Ala Ile Val Val 
305                 310                 315                 320 


Thr Gly Leu His Gln Ser Phe Pro Ala Ile Glu Thr Met Leu Ile Thr 
                325                 330                 335     


Asn Tyr Gln His Ser Gly Ile Gly Gly Asp Phe Ile Phe Pro Val Ala 
            340                 345                 350         


Ala Cys Ala Asn Met Ala Gln Ala Gly Ala Thr Phe Ala Ile Leu Phe 
        355                 360                 365             


Val Thr Lys Asn Ile Lys Thr Lys Ala Leu Ala Ala Pro Ala Gly Val 
    370                 375                 380                 


Ser Ala Ile Leu Gly Ile Thr Glu Pro Ala Leu Phe Gly Ile Asn Leu 
385                 390                 395                 400 


Lys Leu Lys Tyr Pro Phe Phe Ile Ala Leu Gly Ala Ser Ala Ile Gly 
                405                 410                 415     


Ser Leu Phe Met Gly Leu Phe His Val Leu Ala Val Ser Leu Gly Ser 
            420                 425                 430         


Ala Gly Leu Ile Gly Phe Ile Ser Ile Lys Ala Gly Tyr Asn Leu Gln 
        435                 440                 445             


Phe Met Ile Ser Ile Phe Ile Ser Phe Leu Ile Ala Phe Val Val Thr 
    450                 455                 460                 


Ser Ile Tyr Gly Arg Arg Met Glu Ala Lys Ser Ile Thr Lys Glu Lys 
465                 470                 475                 480 


Asn Lys Gln Asn Ala Thr Thr Gln Tyr Gln Pro Glu Lys Val Ile Ile 
                485                 490                 495     


Asp Pro Val Lys Ser Gly Glu Leu Leu Ala Pro Ile Asn Gly Phe Val 
            500                 505                 510         


Ile Pro Leu Ser Asp Val Ser Asp Pro Val Phe Ser Lys Glu Ile Met 
        515                 520                 525             


Gly Lys Gly Ile Ala Ile Lys Pro Lys Ser Gly Glu Leu Phe Ser Pro 
    530                 535                 540                 


Ala Asp Gly Glu Ile Ile Ile Ala Tyr Glu Thr Gly His Ala Tyr Gly 
545                 550                 555                 560 


Ile Lys Thr Lys Asn Gly Gly Glu Val Leu Leu His Ile Gly Ile Asp 
                565                 570                 575     


Thr Val Ser Met Asn Gly Asn Gly Phe Ile Gln Asn Val Lys Val Gly 
            580                 585                 590         


Gln Lys Val Lys Ala Gly Asp Leu Leu Gly Ser Phe Asp Lys Glu Glu 
        595                 600                 605             


Ile Lys Lys Ser Gly Leu Asp Asp Thr Val Ile Ile Val Ile Thr Asn 
    610                 615                 620                 


Ser Ala Ser Tyr Asn Glu Ile Leu Pro Leu Ser Glu Asn Val Asp Ile 
625                 630                 635                 640 


Lys Val Gly Glu Lys Ile Leu Leu Leu Asn 
                645                 650 


<210>  81
<211>  409
<212>  PRT
<213>  Lactococcus lactis

<400>  81

Met Lys Ser Trp Lys Lys Val Ala Leu Gly Gly Ala Ser Val Leu Ala 
1               5                   10                  15      


Leu Ala Thr Leu Ala Ala Cys Gly Ser Ser Ala Ser Ser Asn Lys Ser 
            20                  25                  30          


Ser Ser Ser Ser Ser Ser Asp Ser Ile Lys Gly Thr Val Arg Val Tyr 
        35                  40                  45              


Val Asp Thr Gln Gln Lys Ala Thr Tyr Thr Asp Val Ala Lys Gly Leu 
    50                  55                  60                  


Thr Ser Lys Tyr Pro Asp Leu Lys Val Gln Ile Ile Ala Asn Ala Ser 
65                  70                  75                  80  


Gly Ser Ala Asn Ala Lys Thr Asp Ile Ala Lys Asp Pro Ser Lys Tyr 
                85                  90                  95      


Ala Asp Val Phe Ala Val Pro Asn Asp Gln Leu Gly Asp Met Ala Asp 
            100                 105                 110         


Lys Gly Phe Ile Ser Pro Val Ala Thr Lys Phe Ala Asp Glu Ile Lys 
        115                 120                 125             


Asn Asp Asn Ser Lys Ile Thr Val Ala Gly Val Thr Tyr Lys Asp Lys 
    130                 135                 140                 


Val Tyr Ala Phe Pro Lys Ser Thr Glu Ala Gln Val Leu Phe Tyr Asn 
145                 150                 155                 160 


Lys Ser Lys Leu Ser Ala Asp Asp Val Lys Ser Trp Asp Thr Met Thr 
                165                 170                 175     


Ser Lys Ala Val Phe Ala Thr Asp Phe Thr Asn Ala Tyr Asn Phe Tyr 
            180                 185                 190         


Pro Val Phe Phe Ser Ala Gly Thr Gln Leu Phe Gly Ala Ser Gly Glu 
        195                 200                 205             


Asp Val Thr Gly Thr Asn Val Ala Ser Asp Lys Gly Val Thr Ala Met 
    210                 215                 220                 


Lys Trp Phe Ala Asp Gln Lys Ala Asn Lys Asn Val Met Gln Thr Ser 
225                 230                 235                 240 


Asn Ala Leu Asn Gln Leu Gln Ser Gly Lys Ala Asp Ala Ile Ile Asp 
                245                 250                 255     


Gly Pro Trp Asp Thr Ala Asn Ile Lys Lys Ile Leu Gly Asp Asn Phe 
            260                 265                 270         


Ala Val Ala Pro Tyr Pro Thr Ile Thr Leu Asn Gly Glu Gln Lys Gln 
        275                 280                 285             


Leu Glu Ala Phe Gln Gly Ile Lys Gly Phe Ala Val Asn Ser Ala Thr 
    290                 295                 300                 


Lys Asp Gln Ala Ala Ser Gln Thr Val Ala Gln Tyr Leu Thr Thr Lys 
305                 310                 315                 320 


Ala Ala Gln Leu Lys Leu Phe Asn Ser Gln Gly Asp Val Pro Thr Asn 
                325                 330                 335     


Leu Asp Ala Gln Lys Asp Asp Ala Val Lys Ser Ser Asp Ala Thr Lys 
            340                 345                 350         


Ala Val Ile Thr Met Ala Lys Glu Gly Asn Ser Val Val Met Pro Lys 
        355                 360                 365             


Leu Pro Gln Met Ala Thr Phe Trp Asn Asn Ala Ala Pro Leu Ile Asn 
    370                 375                 380                 


Gly Ala Tyr Thr Gly Ser Ile Lys Ala Thr Asp Tyr Gln Ala Gln Leu 
385                 390                 395                 400 


Gln Lys Phe Gln Asp Ser Ile Ser Lys 
                405                 


<210>  82
<211>  460
<212>  PRT
<213>  Lactococcus lactis

<400>  82

Met Thr Lys Lys Lys Lys Arg Lys Gln Thr Glu Ser Asn Val Ser Pro 
1               5                   10                  15      


Glu Glu Lys Ser Ile Lys Leu Arg Glu Val Phe Gln Lys Gly Asn Thr 
            20                  25                  30          


Val Thr Lys Leu Thr Phe Phe Val Met Gly Leu Asn Gln Ile Lys Asn 
        35                  40                  45              


Lys Gln Trp Val Lys Gly Phe Thr Phe Leu Ile Leu Glu Ile Ala Phe 
    50                  55                  60                  


Ile Gly Trp Leu Leu Phe Ser Gly Leu Ser Ala Phe Ser Leu Leu Ser 
65                  70                  75                  80  


Ser Leu Gly Pro Asn Lys Thr Leu Lys Glu Thr Thr Asp Ala Asn Gly 
                85                  90                  95      


Phe Pro Val Ile Ile Gln Pro Asp His Ser Val Leu Ile Leu Leu Trp 
            100                 105                 110         


Gly Leu Ile Ala Cys Leu Val Val Val Leu Phe Ile Leu Leu Tyr Trp 
        115                 120                 125             


Phe Asn Tyr Arg Ser Asn Lys His Leu Tyr Tyr Leu Glu Arg Glu Gly 
    130                 135                 140                 


Lys His Ile Pro Thr Asn Arg Glu Glu Leu Ala Ser Leu Leu Asp Glu 
145                 150                 155                 160 


Lys Leu Tyr Ala Thr Leu Met Ala Val Pro Leu Ile Gly Val Leu Ala 
                165                 170                 175     


Phe Thr Val Leu Pro Thr Val Tyr Met Ile Ser Met Ala Phe Thr Asn 
            180                 185                 190         


Tyr Asp Arg Leu His Ala Thr Ala Phe Ser Trp Thr Gly Phe Gln Ala 
        195                 200                 205             


Phe Gly Asn Val Leu Thr Gly Asp Leu Ala Gly Thr Phe Phe Pro Val 
    210                 215                 220                 


Leu Gly Trp Thr Leu Val Trp Ala Ile Val Ala Thr Ala Thr Thr Phe 
225                 230                 235                 240 


Leu Gly Gly Val Leu Leu Ala Leu Leu Ile Glu Ser Thr Gly Ile Lys 
                245                 250                 255     


Phe Lys Gly Phe Trp Arg Thr Val Phe Val Ile Val Phe Ala Val Pro 
            260                 265                 270         


Gln Phe Val Thr Leu Leu Met Met Ala Gln Phe Leu Asp Gln Gln Gly 
        275                 280                 285             


Ala Phe Asn Gly Ile Leu Met Asn Leu His Leu Ile Ser Asn Pro Ile 
    290                 295                 300                 


Asn Phe Ile Gly Ala Ala Ser Asp Pro Met Val Ala Arg Ile Thr Val 
305                 310                 315                 320 


Ile Phe Val Asn Met Trp Ile Gly Ile Pro Val Ser Met Leu Val Ser 
                325                 330                 335     


Thr Ala Ile Ile Gln Asn Leu Pro Gln Asp Gln Ile Glu Ala Ala Arg 
            340                 345                 350         


Ile Asp Gly Ala Asn Ser Leu Asn Ile Phe Arg Ser Ile Thr Phe Pro 
        355                 360                 365             


Gln Ile Leu Phe Val Met Thr Pro Ala Leu Ile Gln Gln Phe Ile Gly 
    370                 375                 380                 


Asn Ile Asn Asn Phe Asn Val Ile Tyr Leu Leu Thr Gln Gly Trp Pro 
385                 390                 395                 400 


Met Asn Pro Asn Tyr Gln Gly Ala Gly Ser Thr Asp Leu Leu Val Thr 
                405                 410                 415     


Trp Leu Tyr Asn Leu Val Phe Gly Gln Thr Gln Arg Tyr Asn Ala Ala 
            420                 425                 430         


Ala Val Leu Gly Ile Leu Ile Phe Ile Val Asn Ala Ser Ile Ser Leu 
        435                 440                 445             


Val Ala Tyr Arg Arg Thr Asn Ala Phe Lys Glu Gly 
    450                 455                 460 


<210>  83
<211>  288
<212>  PRT
<213>  Lactococcus lactis

<400>  83

Met Lys Ser Tyr Lys Thr Gln Arg Arg Ile Ser Leu Thr Leu Arg Tyr 
1               5                   10                  15      


Ile Leu Leu Ala Leu Leu Ala Ile Val Trp Ile Phe Pro Ile Ile Trp 
            20                  25                  30          


Ile Val Leu Ala Ser Leu Thr Gln Asn Asn Thr Gly Phe Val Ser Thr 
        35                  40                  45              


Ile Ile Pro Lys Thr Phe Thr Phe Glu Asn Tyr Ile Gln Leu Phe Gln 
    50                  55                  60                  


Asn Lys Ser Gly Ser Phe Pro Phe Val Ser Trp Ile Ile Asn Thr Phe 
65                  70                  75                  80  


Ile Val Ala Val Ile Ser Ala Thr Leu Ser Thr Phe Ile Thr Ile Ile 
                85                  90                  95      


Met Ser Tyr Thr Leu Ser Arg Leu Arg Phe Ala Phe Arg Lys Pro Phe 
            100                 105                 110         


Leu Gln Ile Ala Leu Val Leu Gly Met Phe Pro Gly Phe Met Ser Met 
        115                 120                 125             


Ile Ala Leu Tyr Tyr Ile Leu Lys Ala Met Asn Met Leu Asn Leu Gly 
    130                 135                 140                 


Gly Leu Ile Leu Val Tyr Val Gly Gly Ala Gly Leu Gly Phe Tyr Ile 
145                 150                 155                 160 


Ala Lys Gly Phe Phe Asp Thr Ile Pro Arg Ser Ile Asp Glu Ala Ala 
                165                 170                 175     


Thr Ile Asp Gly Ala Asn Lys Trp Gln Val Phe Thr His Ile Thr Leu 
            180                 185                 190         


Pro Leu Ser Arg Pro Ile Ile Val Tyr Thr Ala Leu Met Ala Phe Ile 
        195                 200                 205             


Ala Pro Trp Thr Asp Phe Ile Phe Ser Gly Ile Ile Leu Gly Asn Asn 
    210                 215                 220                 


Gln Ala His Pro Glu Thr Phe Thr Ile Ala Tyr Gly Leu Tyr Ser Met 
225                 230                 235                 240 


Val His Ser Gln Lys Gly Ala Ala Thr Ala Phe Phe Thr Gln Phe Ile 
                245                 250                 255     


Ala Gly Cys Val Ile Ile Ala Ile Pro Ile Thr Ile Leu Phe Val Ile 
            260                 265                 270         


Met Gln Lys Phe Tyr Val Asn Gly Ile Thr Ala Gly Ala Asp Lys Gly 
        275                 280                 285             


<210>  84
<211>  568
<212>  PRT
<213>  Lactococcus lactis

<400>  84

Met His Lys Leu Ile Glu Leu Ile Glu Lys Gly Lys Pro Phe Phe Glu 
1               5                   10                  15      


Lys Ile Ser Arg Asn Ile Tyr Leu Arg Ala Ile Arg Asp Gly Phe Ile 
            20                  25                  30          


Ala Gly Met Pro Val Ile Leu Phe Ser Ser Ile Phe Ile Leu Ile Ala 
        35                  40                  45              


Tyr Val Pro Asn Ala Trp Gly Phe His Trp Ser Lys Asp Ile Glu Thr 
    50                  55                  60                  


Phe Leu Met Thr Pro Tyr Ser Tyr Ser Met Gly Ile Leu Ala Phe Phe 
65                  70                  75                  80  


Val Gly Gly Thr Thr Ala Lys Ala Leu Thr Asp Ser Lys Asn Arg Asp 
                85                  90                  95      


Leu Pro Ala Thr Asn Gln Ile Asn Phe Leu Ser Thr Met Leu Ala Ser 
            100                 105                 110         


Met Val Gly Phe Leu Leu Met Ala Ala Glu Pro Ala Lys Glu Gly Gly 
        115                 120                 125             


Phe Leu Thr Ala Phe Met Gly Thr Lys Gly Leu Leu Thr Ala Phe Ile 
    130                 135                 140                 


Ala Ala Phe Val Thr Val Asn Val Tyr Lys Val Cys Val Lys Asn Asn 
145                 150                 155                 160 


Val Thr Ile Arg Met Pro Glu Asp Val Pro Pro Asn Ile Ser Gln Val 
                165                 170                 175     


Phe Lys Asp Leu Ile Pro Phe Thr Val Ser Val Val Leu Leu Tyr Gly 
            180                 185                 190         


Leu Glu Leu Leu Val Lys Gly Thr Leu Gly Val Thr Val Ala Glu Ser 
        195                 200                 205             


Ile Gly Thr Leu Ile Ala Pro Leu Phe Ser Ala Ala Asp Gly Tyr Leu 
    210                 215                 220                 


Gly Ile Thr Leu Ile Phe Gly Ala Tyr Ala Phe Phe Trp Phe Val Gly 
225                 230                 235                 240 


Ile His Gly Pro Ser Ile Val Glu Pro Ala Ile Ala Ala Ile Thr Tyr 
                245                 250                 255     


Ala Asn Ile Asp Val Asn Leu His Leu Ile Gln Ala Gly Gln His Ala 
            260                 265                 270         


Asp Lys Val Ile Thr Ser Gly Thr Gln Met Phe Ile Ala Thr Met Gly 
        275                 280                 285             


Gly Thr Gly Ala Thr Leu Ile Val Pro Phe Leu Phe Met Trp Ile Cys 
    290                 295                 300                 


Lys Ser Asp Arg Asn Arg Ala Ile Gly Arg Ala Ser Val Val Pro Thr 
305                 310                 315                 320 


Phe Phe Gly Val Asn Glu Pro Ile Leu Phe Gly Ala Pro Ile Val Leu 
                325                 330                 335     


Asn Pro Ile Phe Phe Val Pro Phe Ile Phe Thr Pro Ile Val Asn Val 
            340                 345                 350         


Trp Ile Phe Lys Phe Phe Val Asp Thr Leu Asn Met Asn Ser Phe Ser 
        355                 360                 365             


Ala Asn Leu Pro Trp Val Thr Pro Gly Pro Leu Gly Ile Val Leu Gly 
    370                 375                 380                 


Thr Asn Phe Gln Val Leu Ser Phe Ile Leu Ala Gly Leu Leu Val Val 
385                 390                 395                 400 


Val Asp Thr Ile Ile Tyr Tyr Pro Phe Val Lys Val Tyr Asp Glu Gln 
                405                 410                 415     


Ile Leu Glu Glu Glu Arg Ser Gly Lys Thr Asn Asp Ala Leu Lys Glu 
            420                 425                 430         


Lys Val Ala Glu Asn Phe Asn Thr Ala Lys Ala Asp Ala Val Leu Gly 
        435                 440                 445             


Lys Ala Gly Val Ala Lys Glu Asp Val Ala Ala Asn Asn Asn Ile Thr 
    450                 455                 460                 


Lys Glu Thr Asn Val Leu Val Leu Cys Ala Gly Gly Gly Thr Ser Gly 
465                 470                 475                 480 


Leu Leu Ala Asn Ala Leu Asn Lys Ala Ala Ala Glu Tyr Asn Val Pro 
                485                 490                 495     


Val Lys Ala Ala Ala Gly Gly Tyr Gly Ala His Arg Glu Met Leu Pro 
            500                 505                 510         


Glu Phe Asp Leu Val Ile Leu Ala Pro Gln Val Ala Ser Asn Phe Asp 
        515                 520                 525             


Asp Met Lys Ala Glu Thr Asp Lys Leu Gly Ile Lys Leu Val Lys Thr 
    530                 535                 540                 


Glu Gly Ala Gln Tyr Ile Lys Leu Thr Arg Asp Gly Lys Gly Ala Leu 
545                 550                 555                 560 


Ala Phe Val Gln Gln Gln Phe Asp 
                565             


<210>  85
<211>  215
<212>  PRT
<213>  Lactococcus lactis

<400>  85

Met Thr Ile Lys Phe Lys His Ala Tyr Lys Ser Phe Gly Lys Lys Ile 
1               5                   10                  15      


Ile Phe Lys Asp Ala Ser Ile Asn Ile Asn Arg Asn Ser Ile Tyr Phe 
            20                  25                  30          


Ile Met Ala Pro Asn Gly Ser Gly Lys Thr Thr Phe Phe Lys Ile Ile 
        35                  40                  45              


Thr Asn Leu Gln Thr Leu Asp Lys Gly Lys Val Tyr Asn Asp Cys Ser 
    50                  55                  60                  


Asn Arg Lys Gln Phe Ser Ile Phe Asp Asp Leu Ser Leu Tyr Lys Asn 
65                  70                  75                  80  


Leu Thr Gly Tyr Gln Asn Ile Gln Leu Phe Thr Asn Phe Lys Phe Asn 
                85                  90                  95      


Lys Phe Glu Ile Glu Gln His Ser Lys Lys Tyr Glu Met Leu Ser Lys 
            100                 105                 110         


Leu Asn Gln Lys Val Ser Thr Tyr Ser Leu Gly Glu Gly Lys Lys Ile 
        115                 120                 125             


Ser Leu Leu Leu Trp Glu Leu Leu Asn Pro Asp Leu Val Ile Met Asp 
    130                 135                 140                 


Glu Val Thr Asn Gly Leu Asp His Asn Thr Leu Lys Glu Leu Lys Ser 
145                 150                 155                 160 


Ser Leu Leu Lys Ala Lys Glu Asp Ser Ile Ile Ile Leu Thr Gly His 
                165                 170                 175     


Glu Leu Leu Phe Tyr Glu Glu Ile Ile Asp Asp Leu Tyr Ile Leu Asn 
            180                 185                 190         


Asn Gly Lys Leu Leu Lys Glu Leu Asn Trp Lys Glu Glu Gly Leu Thr 
        195                 200                 205             


Lys Thr Tyr Glu Lys Tyr Phe 
    210                 215 


<210>  86
<211>  462
<212>  PRT
<213>  Lactococcus lactis

<400>  86

Met Lys Glu Gly Lys Met Lys Gln Arg Leu Ser Tyr Ala Phe Gly Ala 
1               5                   10                  15      


Leu Gly His Asp Val Tyr Tyr Tyr Ser Ile Ser Thr Phe Phe Ile Ala 
            20                  25                  30          


Phe Val Thr Ala Gln Met Phe Ala Gly Thr Pro His Glu Asp Ala Met 
        35                  40                  45              


Ile Ala Leu Val Thr Ser Leu Val Val Ile Ile Arg Leu Ile Glu Ile 
    50                  55                  60                  


Ile Phe Asp Pro Ile Ile Gly Ser Ile Ile Asp Asn Thr His Thr Arg 
65                  70                  75                  80  


Trp Gly Lys Phe Lys Pro Trp Leu Val Val Gly Gly Ile Met Ser Ser 
                85                  90                  95      


Leu Met Ile Met Leu Met Phe Ser Asp Phe Phe Gly Leu Ala Lys Ser 
            100                 105                 110         


Asp Asn Arg Thr Leu Phe Ala Ile Val Phe Ile Ile Ala Phe Ile Ile 
        115                 120                 125             


Leu Asp Ala Phe Tyr Ser Phe Lys Asp Ile Ala Phe Trp Ser Met Ile 
    130                 135                 140                 


Pro Ala Leu Ser Glu Lys Asn Ser Glu Arg Glu Thr Leu Gly Thr Phe 
145                 150                 155                 160 


Ala Arg Phe Gly Ser Ala Ile Gly Ala Gln Gly Ala Thr Ile Leu Ala 
                165                 170                 175     


Ile Pro Ile Thr Ile Phe Phe Thr Lys Gly Gly His Ala Gln Gly Ala 
            180                 185                 190         


Arg Gly Phe Phe Ala Phe Gly Val Ile Ala Ala Leu Val Gln Gly Ile 
        195                 200                 205             


Ser Ala Leu Val Thr Ala Trp Gly Thr Lys Glu Gln Lys Ser Val Ile 
    210                 215                 220                 


Arg Gln Glu Gly Thr Lys Thr Asn Thr Leu Asp Val Phe Lys Ala Leu 
225                 230                 235                 240 


Leu Lys Asn Asp Gln Leu Met Trp Leu Ser Leu Ser Tyr Ile Leu Phe 
                245                 250                 255     


Ala Ile Ala Tyr Val Ala Thr Thr Ala Thr Leu Ile Leu Asn Phe Thr 
            260                 265                 270         


Phe Val Ile Gly Asn Ala Ser Leu Tyr Ser Ile Thr Gly Ile Val Gly 
        275                 280                 285             


Phe Ile Gly Ser Ile Ile Leu Val Pro Met Phe Pro Ile Leu Ala Lys 
    290                 295                 300                 


Lys Phe Gly Arg Arg Lys Val Leu Thr Gly Ala Ile Ile Ser Met Leu 
305                 310                 315                 320 


Leu Gly Tyr Leu Leu Phe Val Leu Gly Ser Ser Val Ala Met Thr Val 
                325                 330                 335     


Ala Gly Leu Ile Phe Leu Thr Ala Pro Tyr Gln Leu Val Phe Leu Ser 
            340                 345                 350         


Val Leu Met Thr Ile Thr Asp Ser Val Glu Tyr Gly Gln Trp Lys Asn 
        355                 360                 365             


Gly Val Arg Asn Glu Ala Val Thr Leu Ala Met Arg Pro Leu Leu Asp 
    370                 375                 380                 


Lys Ile Ala Gly Ala Phe Ser Asn Gly Ile Tyr Gly Phe Val Ala Ile 
385                 390                 395                 400 


Ser Ala Gly Met Thr Gly Ser Lys Tyr Ile Ala Gly His Thr Tyr Gly 
                405                 410                 415     


Val Ala Thr Phe Lys Leu Tyr Ser Phe Val Val Pro Ala Ile Leu Met 
            420                 425                 430         


Ile Ile Ala Leu Ala Val Tyr Leu Phe Lys Val Lys Leu Thr Glu Lys 
        435                 440                 445             


Arg His Glu Glu Ile Val Ala Glu Leu Glu Glu Arg Leu Lys 
    450                 455                 460         


<210>  87
<211>  573
<212>  PRT
<213>  Lactococcus lactis

<400>  87

Met Ile Asp Leu Val Ile Gly Lys Ile Pro Leu Ala Asn Trp Val Ser 
1               5                   10                  15      


Ser Ala Thr Asp Trp Ile Thr Ser Thr Phe Ser Ser Gly Phe Asp Val 
            20                  25                  30          


Ile Gln Lys Ser Gly Thr Val Leu Met Asn Gly Ile Thr Gly Ala Leu 
        35                  40                  45              


Thr Ala Val Pro Phe Trp Leu Met Ile Ala Val Val Thr Ile Leu Ala 
    50                  55                  60                  


Ile Leu Val Ser Gly Lys Lys Phe Ala Phe Pro Leu Phe Ala Phe Ile 
65                  70                  75                  80  


Gly Leu Cys Leu Ile Ala Asn Gln Gly Leu Trp Ser Asp Leu Met Ser 
                85                  90                  95      


Thr Ile Thr Leu Val Leu Leu Ser Ser Leu Leu Ser Ile Ile Ile Gly 
            100                 105                 110         


Val Pro Leu Gly Ile Trp Met Ala Lys Ser Glu Leu Val Ala Lys Ile 
        115                 120                 125             


Val Gln Pro Ile Leu Asp Phe Met Gln Thr Met Pro Gly Phe Val Tyr 
    130                 135                 140                 


Leu Ile Pro Ala Val Ala Phe Phe Gly Ile Gly Val Val Pro Gly Val 
145                 150                 155                 160 


Phe Ala Ser Val Ile Phe Ala Leu Pro Pro Thr Val Arg Met Thr Asn 
                165                 170                 175     


Leu Gly Ile Arg Gln Val Ser Thr Glu Leu Val Glu Ala Ala Asp Ser 
            180                 185                 190         


Phe Gly Ser Thr Ala Arg Gln Lys Leu Phe Lys Leu Glu Phe Pro Leu 
        195                 200                 205             


Ala Lys Gly Thr Ile Met Ala Gly Val Asn Gln Thr Ile Met Leu Ala 
    210                 215                 220                 


Leu Ser Met Val Val Ile Ala Ser Met Ile Gly Ala Pro Gly Leu Gly 
225                 230                 235                 240 


Arg Gly Val Leu Ala Ala Val Gln Ser Ala Asp Ile Gly Lys Gly Phe 
                245                 250                 255     


Val Ser Gly Ile Ser Leu Val Ile Leu Ala Ile Ile Ile Asp Arg Phe 
            260                 265                 270         


Thr Gln Lys Leu Asn Val Ser Pro Leu Glu Lys Gln Gly Asn Pro Lys 
        275                 280                 285             


Leu Lys Lys Trp Lys Arg Trp Ile Ala Ile Val Ser Leu Leu Ala Leu 
    290                 295                 300                 


Ile Val Gly Ala Phe Ser Gly Met Ser Phe Gly Lys Lys Ser Ser Asp 
305                 310                 315                 320 


Lys Lys Val Asp Leu Val Tyr Met Asn Trp Asp Ser Glu Val Ala Ser 
                325                 330                 335     


Ile Asn Val Leu Thr Gln Ala Met Glu Glu His Ser Phe Asp Val Thr 
            340                 345                 350         


Thr Thr Ala Leu Asp Asn Ala Val Ala Trp Gln Thr Val Ala Asn Ser 
        355                 360                 365             


Gln Ala Asp Gly Met Val Ser Ala Trp Leu Pro Asn Thr His Lys Thr 
    370                 375                 380                 


Gln Trp Lys Lys Tyr Gly Lys Ser Val Glu Leu Leu Gly Pro Asn Leu 
385                 390                 395                 400 


Lys Gly Ala Lys Val Gly Phe Val Val Pro Ser Tyr Met Asn Val Asn 
                405                 410                 415     


Ser Ile Glu Asp Leu Thr Asn Gln Ala Asn Lys Thr Ile Thr Gly Ile 
            420                 425                 430         


Glu Pro Gly Ala Gly Val Met Ala Ala Ser Glu Asn Thr Leu Lys Ser 
        435                 440                 445             


Tyr Ser Asn Leu Lys Asp Trp Lys Leu Val Pro Ser Ser Ser Gly Ala 
    450                 455                 460                 


Met Thr Val Ala Leu Gly Glu Ala Ile Lys Gln His Lys Asp Ile Val 
465                 470                 475                 480 


Ile Thr Gly Trp Ser Pro His Trp Ile Phe Asn Lys Tyr Asp Leu Lys 
                485                 490                 495     


Tyr Leu Ala Asp Pro Lys Gly Thr Met Gly Thr Ser Glu Asn Ile Asn 
            500                 505                 510         


Thr Ile Val Arg Lys Gly Leu Lys Lys Glu Asn Pro Glu Ala Tyr Lys 
        515                 520                 525             


Val Leu Asn Asn Phe Asn Trp Thr Thr Lys Asp Met Glu Ser Val Met 
    530                 535                 540                 


Leu Asp Ile Gln Asn Gly Lys Thr Pro Glu Ala Ala Ala Lys Ala Trp 
545                 550                 555                 560 


Ile Lys Asp His Gln Lys Gln Val Asp Lys Trp Phe Lys 
                565                 570             


<210>  88
<211>  474
<212>  PRT
<213>  Escherichia coli

<400>  88

Met Ser Arg Leu Val Val Val Ser Asn Arg Ile Ala Pro Pro Asp Glu 
1               5                   10                  15      


His Ala Ala Ser Ala Gly Gly Leu Ala Val Gly Ile Leu Gly Ala Leu 
            20                  25                  30          


Lys Ala Ala Gly Gly Leu Trp Phe Gly Trp Ser Gly Glu Thr Gly Asn 
        35                  40                  45              


Glu Asp Gln Pro Leu Lys Lys Val Lys Lys Gly Asn Ile Thr Trp Ala 
    50                  55                  60                  


Ser Phe Asn Leu Ser Glu Gln Asp Leu Asp Glu Tyr Tyr Asn Gln Phe 
65                  70                  75                  80  


Ser Asn Ala Val Leu Trp Pro Ala Phe His Tyr Arg Leu Asp Leu Val 
                85                  90                  95      


Gln Phe Gln Arg Pro Ala Trp Asp Gly Tyr Leu Arg Val Asn Ala Leu 
            100                 105                 110         


Leu Ala Asp Lys Leu Leu Pro Leu Leu Gln Asp Asp Asp Ile Ile Trp 
        115                 120                 125             


Ile His Asp Tyr His Leu Leu Pro Phe Ala His Glu Leu Arg Lys Arg 
    130                 135                 140                 


Gly Val Asn Asn Arg Ile Gly Phe Phe Leu His Ile Pro Phe Pro Thr 
145                 150                 155                 160 


Pro Glu Ile Phe Asn Ala Leu Pro Thr Tyr Asp Thr Leu Leu Glu Gln 
                165                 170                 175     


Leu Cys Asp Tyr Asp Leu Leu Gly Phe Gln Thr Glu Asn Asp Arg Leu 
            180                 185                 190         


Ala Phe Leu Asp Cys Leu Ser Asn Leu Thr Arg Val Thr Thr Arg Ser 
        195                 200                 205             


Ala Lys Ser His Thr Ala Trp Gly Lys Ala Phe Arg Thr Glu Val Tyr 
    210                 215                 220                 


Pro Ile Gly Ile Glu Pro Lys Glu Ile Ala Lys Gln Ala Ala Gly Pro 
225                 230                 235                 240 


Leu Pro Pro Lys Leu Ala Gln Leu Lys Ala Glu Leu Lys Asn Val Gln 
                245                 250                 255     


Asn Ile Phe Ser Val Glu Arg Leu Asp Tyr Ser Lys Gly Leu Pro Glu 
            260                 265                 270         


Arg Phe Leu Ala Tyr Glu Ala Leu Leu Glu Lys Tyr Pro Gln His His 
        275                 280                 285             


Gly Lys Ile Arg Tyr Thr Gln Ile Ala Pro Thr Ser Arg Gly Asp Val 
    290                 295                 300                 


Gln Ala Tyr Gln Asp Ile Arg His Gln Leu Glu Asn Glu Ala Gly Arg 
305                 310                 315                 320 


Ile Asn Gly Lys Tyr Gly Gln Leu Gly Trp Thr Pro Leu Tyr Tyr Leu 
                325                 330                 335     


Asn Gln His Phe Asp Arg Lys Leu Leu Met Lys Ile Phe Arg Tyr Ser 
            340                 345                 350         


Asp Val Gly Leu Val Thr Pro Leu Arg Asp Gly Met Asn Leu Val Ala 
        355                 360                 365             


Lys Glu Tyr Val Ala Ala Gln Asp Pro Ala Asn Pro Gly Val Leu Val 
    370                 375                 380                 


Leu Ser Gln Phe Ala Gly Ala Ala Asn Glu Leu Thr Ser Ala Leu Ile 
385                 390                 395                 400 


Val Asn Pro Tyr Asp Arg Asp Glu Val Ala Ala Ala Leu Asp Arg Ala 
                405                 410                 415     


Leu Thr Met Ser Leu Ala Glu Arg Ile Ser Arg His Ala Glu Met Leu 
            420                 425                 430         


Asp Val Ile Val Lys Asn Asp Ile Asn His Trp Gln Glu Cys Phe Ile 
        435                 440                 445             


Ser Asp Leu Lys Gln Ile Val Pro Arg Ser Ala Glu Ser Gln Gln Arg 
    450                 455                 460                 


Asp Lys Val Ala Thr Phe Pro Lys Leu Ala 
465                 470                 


<210>  89
<211>  266
<212>  PRT
<213>  Escherichia coli

<400>  89

Met Thr Glu Pro Leu Thr Glu Thr Pro Glu Leu Ser Ala Lys Tyr Ala 
1               5                   10                  15      


Trp Phe Phe Asp Leu Asp Gly Thr Leu Ala Glu Ile Lys Pro His Pro 
            20                  25                  30          


Asp Gln Val Val Val Pro Asp Asn Ile Leu Gln Gly Leu Gln Leu Leu 
        35                  40                  45              


Ala Thr Ala Ser Asp Gly Ala Leu Ala Leu Ile Ser Gly Arg Ser Met 
    50                  55                  60                  


Val Glu Leu Asp Ala Leu Ala Lys Pro Tyr Arg Phe Pro Leu Ala Gly 
65                  70                  75                  80  


Val His Gly Ala Glu Arg Arg Asp Ile Asn Gly Lys Thr His Ile Val 
                85                  90                  95      


His Leu Pro Asp Ala Ile Ala Arg Asp Ile Ser Val Gln Leu His Thr 
            100                 105                 110         


Val Ile Ala Gln Tyr Pro Gly Ala Glu Leu Glu Ala Lys Gly Met Ala 
        115                 120                 125             


Phe Ala Leu His Tyr Arg Gln Ala Pro Gln His Glu Asp Ala Leu Met 
    130                 135                 140                 


Thr Leu Ala Gln Arg Ile Thr Gln Ile Trp Pro Gln Met Ala Leu Gln 
145                 150                 155                 160 


Gln Gly Lys Cys Val Val Glu Ile Lys Pro Arg Gly Thr Ser Lys Gly 
                165                 170                 175     


Glu Ala Ile Ala Ala Phe Met Gln Glu Ala Pro Phe Ile Gly Arg Thr 
            180                 185                 190         


Pro Val Phe Leu Gly Asp Asp Leu Thr Asp Glu Ser Gly Phe Ala Val 
        195                 200                 205             


Val Asn Arg Leu Gly Gly Met Ser Val Lys Ile Gly Thr Gly Ala Thr 
    210                 215                 220                 


Gln Ala Ser Trp Arg Leu Ala Gly Val Pro Asp Val Trp Ser Trp Leu 
225                 230                 235                 240 


Glu Met Ile Thr Thr Ala Leu Gln Gln Lys Arg Glu Asn Asn Arg Ser 
                245                 250                 255     


Asp Asp Tyr Glu Ser Phe Ser Arg Ser Ile 
            260                 265     


<210>  90
<211>  7145
<212>  DNA
<213>  Lactococcus lactis

<400>  90
tatgttgttg aagaaatgaa gaaaaataat tataacttcg gtggtgaaca atctggtcat       60

atgattttcc ttgattacaa cacgactgga gatggccaac tttcagctat tcaattatta      120

aaagtaatgc gtgaaacggg taaaacttta tctgaattag caagtgaagt tacaatttat      180

cctcaaaaat tagttaatgt tcgtgtaaaa gacaatgcag ctaagaaatc agcgatggat      240

gtgccagcta ttcagaaagt tatttcagaa atggaaactt caatgaatgg taaaggacgt      300

attttagttc gcccttcagg tactgagccc cttttacgtg tgatggctga agctccaact      360

catgaacaag ttgaccatgt tgtggataca attgttgaag ttgttgaaga ggaaattggt      420

gtgaaataaa gaaaagacaa ggagaatatt cttcttgtct tttttcatat cctaaaactc      480

tacctactgt ggtagagttt ttttatcttt tttggcgtct agcaaactct gtaaaacgaa      540

aacggtcaac ctgatgtcgt gattcagtat actggaaaag tgtattatcg gcaagataga      600

catgagattt cacggaaaca acatgatggt ccttcggatt taaatcaaga tatgtaaaat      660

catcttcaca agcaaaatca atggtaactt ctttttgggc ataggcaata tcaagtccca      720

aagccccttc taaataatcg taagtagaat tttgggcatg tgcgggggtc aaaccatcag      780

cgtatttttc taaaaataaa tcccaatcca aaatggaaaa tttaccatct acttttcttc      840

ttctaagaat actaagggct tggtcgccga tggcgaatcc agtagtttct gaaagagctg      900

gggtaatttt tatactttca aattttatta cttcagtttc actgtgaaaa cccattgaag      960

tttgcaattc tttatatgaa gttaagccgg aaatagggaa aaggagccga tcgtgagcga     1020

ggacaatgct gccatagcca tgtcttcttt ggatgagccc tttttcttct aaaattttta     1080

aagcttgtct gacggttgaa cggctacttt cataactaat agaaagttca ttctcgcttg     1140

gaagaatatc gttcgtttta tagatatcat taaaaatctt tttttctaaa tcttgcaaaa     1200

tcacttcata tttcttcata ctttatattt tatcataaaa ataattgtta acgcttgctg     1260

aaaacgtttt tatgtgatac aatgattttg ttaacttgta cgtacaactg taaaagatag     1320

gagattctta tgtttggaat aggaaaaaag aaagaattga gagatgataa aagcctttat     1380

gctccagttt ctggggaagt tatcaacctt tcaacagtca acgaccccgt attttcaaaa     1440

aagataatgg gagacgggtt cgcggttgag ccaaaagaaa ataaaatttt tgccccagtt     1500

tctgcaaaag taactttggt tcaaggacat gcaattggtt ttaaacgtgc tgatggctta     1560

gatgtacttt tacatcttgg aattgataca gtagctctta aaggtcttca ttttaaaatc     1620

aaggtcaaag ttgatgatat tgtcaatggt ggtgatgagc ttggaagcgt tgattgggca     1680

cagattgaag ctgcaggttt agataaaacg acaatggtta tctttacaaa tacaaaagat     1740

aaactctctg agttcaatgt caattatgga ccagctactt ctggaagtga acttggtaag     1800

gcaagtgtta aataaagaat aaaaatttaa ggttaatcca tttataaggc aaaggtgacg     1860

agggctttgt atataaattt tgtgactatt aaaatcgtca attattattt tatattatga     1920

aatacttcat aaataatgaa gtaaaaggag aacttatggc aaattattca caacttgcga     1980

cagaaattat cgcaaatgta ggtggcgctg agaatgtcac aaaagttatt cactgtatca     2040

ctcgtcttcg ttttaccttg aaagacaaag ataaagcaga tacggcggcg attgaagcct     2100

tacctggtgt cgctggagct gtttataact caaacttgaa tcaatatcaa gtagttattg     2160

gacaagctgt agaagatgtt tatgacgagg ttgttgaaca gcttggagat tcagttgttg     2220

atgaagatgc aacggcgcaa gcacttgctg caacagcacc ggctagtggt aaaaaacaaa     2280

atccaattgt tcatgctttc caagtggtta ttgggacaat tacaggttcg atgattccaa     2340

ttattggttt acttgcggct ggtgggatga ttaatggatt attaagtatc tttgttaaag     2400

gaaatcgttt aattgaagtg attgaccctg caagttcaac ttacgtcatt atctcaactc     2460

tagcaatgac accattttat ttcttacctg ttttagtagg attttcagca gcaaaacaat     2520

tagcacctaa agatactgtt ttacaattta ttggtgctgc tgttggtggt ttcatgatta     2580

atccagggat tactaacttg gtaaatgctc atgttggaac aaatgcggcc ggtaaaaatg     2640

ttgttgttga agcagcagct ccagtagcaa atttccttgg agtcactttt aatacaagtt     2700

attttggaat tccggttgct ttgccaagtt atgcttatac aattttccca atcattgtgg     2760

cggtagcaat cgctaaacct ttgaatgctt ggttgaaaaa ggttttacca cttgccttgc     2820

gtccaatttt ccaaccgatg attactttct tcatcactgc ttcaatcatt ttactcttgg     2880

tcggtcctgt tatttcaaca atttcatctg gtttgtcatt cgttattgac catatcttgt     2940

cattaaactt agggattgca agtattatcg tcggtggttt gtatcaatgt ttggttatat     3000

ttggtttgca ctggttggtt gtaccactta tttcacaaga gttggcagca acaggagcaa     3060

gctcacttaa tatgattgtt agcttcacaa tgcttgcgca aggagttggt gccttgactg     3120

tcttctttaa atctaaaaaa gctgacctta aaggactttc tgctccagct gccatttcgg     3180

ctttttgtgg agtaactgaa cctgccatgt acggaattaa cttgaaatat gttcgcgtct     3240

tcatcatgtc ttcaattggt gcagcaattg gtgctgggat tgccggattt ggtggcttac     3300

aaatgtttgg attttcaggg tcattgatta gttttcctaa ctttatctct aatccattga     3360

cgcatcatgc acctgcgggt aacttaatgc tcttctggat tgccactgcg gtatgtgctg     3420

ttgccacttt cttattagtt tggttctttg gttacaagga tactgatgtc atgggacaag     3480

gagttgaaca aaaaaatgca tttaaggatg ctgtaaaata attaacagaa ttgtttggga     3540

gcagagcgaa taaattctgc ttcctttagt ttaataaagg gggagaaatg actgataaag     3600

attggatgat ccaatatgac aaacaagaag tagggaagcg ttcttatggg caagaatctt     3660

taatgtcatt gggaaatggt tatttagggc ttcgtggggc gcctttgtgg gcaacttgtt     3720

cggataatca ttatccggga ctctatgtcg caggggtctt taatcacaca agtacagaag     3780

ttgcaggtca tgatgttatc aacgaagata tggtcaattg gccaaatcca caattgatta     3840

aggtttatat tgataatgaa ttggttgact tcgaggcagc aattgagaaa aattcttcga     3900

ttgatttcaa aaatggattg caaattgaga gctataatgt cagtttagcc aagggaggtt     3960

tgactttagt gaccacaaaa tttgttgatc ccatccattt tcacgatttt gggttcgttg     4020

gagaaatcat cgctgatttt tctggaaaat tgcgaataga aacttttatt gatggttcgg     4080

tattgaatca aaatgttgaa cgctatcggg cttttgacag caaagaattt gaagtgactc     4140

aaattgctga tggacttttg gtggcaaaaa ctagaacgac ggacatagaa ttagcagttg     4200

cgactaaaac ttatttaaat ggtcagccat tgaaaaaagt agaatctgga aattctgaaa     4260

tttttaaaga atccattgaa gttgatttac taaaaaacca agaagttcag tttgaaaaat     4320

cgattgttat tgctagttct tatgaaacca aaaaccctgt tgaatttgtg ctgacagaac     4380

tggcagcaac ttctgtcagt aaaattcagg aaaataatgc aaattattgg gagaaagtat     4440

ggcaggatgg cgatattgtc atcgaatctg atcatgcgga tttgcaaaga atggtgcgaa     4500

tgaatatttt ccatattcgc caagcggcac aacacggtgc taatcagttt ttagatgcgt     4560

ccgtaggttc gcgtggattg actggtgaag gttatcgagg acatattttc tgggatgaaa     4620

tttttgttct accttattat gcggcgaatg aaccagaaac agcgcgtgat ttgcttttgt     4680

accgaatcaa tcgattgact gctgcacagg aaaatgcaaa ggttgatgga gaaatagggg     4740

caatgtttcc ttggcaatcc ggcttaattg gggatgaaca ggcacaattt gttcatttga     4800

atacagtaaa taatgaatgg gaaccagata atagtcgccg tcaaagacat gtcagcttag     4860

ctattgttta caatctgtgg atttacttac agctgacaga tgatgaaagt attttgactg     4920

acggtggact ggatttgctc gttgaaacca cgaagttttg gttaaacaaa gcagaattgg     4980

gaagtgatag ccgctatcat atcgctggtg tcatgggtcc tgatgaatat catgaggctt     5040

atccagggca agaaggtggt atttgcgata atgcttatac gaatttgatg ctgacttggc     5100

agttaaattg gctgacagag ctgtcagtga aaggttttga aattccagca gatttgcttg     5160

aagagtcaca aaaggttcgg gaaaatcttt atttagatat tgatgagaat ggtgtgattg     5220

cccaatatgc taagtatttt gagcttaaag aagttgattt tgcagcttat gaagcaaaat     5280

atggcgatat tcatcggatt gaccgtttga tgaaggctga gggaatttcg cctgacgaat     5340

atcaagtggc taaacaagct gataccttga tgttaatgta caatttgggt catgaacatg     5400

tgatcaaatt ggtcaaacaa ttaggttatg agctacccaa aaattggttg aaagttaatc     5460

gtgattatta tcttgcacga actgtccatg gttcaacgac atctcgtcca gtttttgctg     5520

ggattgatgt caaattgggt gattttgatg aagcgcttga ctttttaatc actgcgattg     5580

gaagtgatta ctatgatatt caaggcggaa ccacggccga aggggttcac attggggtca     5640

tgggagaaac acttgaagtg attcaaaatg aatttgccgg tttgacacta cgcgatggat     5700

acttttcaat tgctccgcat ttaccaaaaa gttggaccaa attgaaattc agtcaaattt     5760

tcaaaggttg tcaagtggaa attttgattg aaaaaggtca attattactg acagcttcat     5820

cagacttgct gattaaagtt tatgatgagg aagttcagtt aaaagcagga gtacaagcta     5880

attttgattt aaaataaata gttttgctct taataaagtt ttgatacaag gatttacaat     5940

tattttttga taaaaaaatt actgatagaa atgaaaaaaa ttctgtcagt aattttggaa     6000

agtcattcta aaaaattcat tttaaaatga cgagaaagaa ggtaaaaaga tgtttaaagc     6060

agtattgttt gatttagatg gcgtaattac agataccgca gagtatcatt atagagcatg     6120

gaaagcttta gctgaggaaa ttggtattaa tggtgtcgat cgtcagttta atgagcaact     6180

taaaggagtt tctcgtgaag attctctaca gaaaatttta gatttggctg gcaaaaaagt     6240

atcggcagaa gaatttaagg cactagctca aagaaaaaat gataactatg tgactatgat     6300

tcaagatgtt tccccagctg atgtttatcc tggaatctta caattactca aagatttgcg     6360

ttctaatcat atcaagattg ctttagcttc tgcctcaaaa aatgggccat ttttattgga     6420

acggatggcc ttgactgaat attttgatgc gattgcagat ccagcagaag ttgcggcatc     6480

taaaccagct ccagatattt ttattgcggc agctcatgcc gttggtgtaa aaccgactga     6540

atcaattggt ttagaggatt ctcaagctgg aattcaagcg attaaagatt caggggcgct     6600

accaattgga gttgggcgac cagaggattt gggaaatgat atcgtcgttg tagctgacac     6660

ttctcattat actttggatt ttcttaaaga agtttggtta aaagaaaaag gatagtttct     6720

atttaaaacg accaaaattt tgtcaaaaaa aatgccccag ttgagtgggc attttaattt     6780

tttatttgtc tttctaacca atctgaggcc caagagaacc aacgatgaac tgaatcaaga     6840

caataagcat cagaaggagc tgttgtccga ttcgccaagg aaatgccatg tggtcctgct     6900

tcaaaaaaat gagcttcaaa tgggacttgg tgtttggaca ggcggtcaca atatttaaga     6960

ctgttataga taggaacact tgcatcatca gcggtatgcc agatgaaagt agggggtgta     7020

gccgaagtca ctttttctga aatattgtat tctgaaatat tttcaatttc aaagttaaaa     7080

tgtgataaat cacttggcca accaaaagtg aaggaagtaa cagggtaaca aagaattaca     7140

ccctt                                                                 7145


