                         SEQUENCE LISTING

<110>  The Regents of the University of California
       Novartis AG
       Spradlin, Jessica
       Ward, Carl C.
       Nomura, Daniel K.
       Schirle, Markus
       Tallarico, John A.
       McKenna, Jeffrey
       Maimone, Thomas John
       Hu, Xirui
 
<120>  COVALENT TARGETING OF E3 LIGASES

<130>  052103-517001WO

<150>  US 62/743,337
<151>  2018-10-09

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  1

Met Ser Thr Arg Lys Arg Arg Gly Gly Ala Ile Asn Ser Arg Gln Ala 
1               5                   10                  15      


Gln Lys Arg Thr Arg Glu Ala Thr Ser Thr Pro Glu Ile Ser Leu Glu 
            20                  25                  30          


Ala Glu Pro Ile Glu Leu Val Glu Thr Ala Gly Asp Glu Ile Val Asp 
        35                  40                  45              


Leu Thr Cys Glu Ser Leu Glu Pro Val Val Val Asp Leu Thr His Asn 
    50                  55                  60                  


Asp Ser Val Val Ile Val Asp Glu Arg Arg Arg Pro Arg Arg Asn Ala 
65                  70                  75                  80  


Arg Arg Leu Pro Gln Asp His Ala Asp Ser Cys Val Val Ser Ser Asp 
                85                  90                  95      


Asp Glu Glu Leu Ser Arg Asp Arg Asp Val Tyr Val Thr Thr His Thr 
            100                 105                 110         


Pro Arg Asn Ala Arg Asp Glu Gly Ala Thr Gly Leu Arg Pro Ser Gly 
        115                 120                 125             


Thr Val Ser Cys Pro Ile Cys Met Asp Gly Tyr Ser Glu Ile Val Gln 
    130                 135                 140                 


Asn Gly Arg Leu Ile Val Ser Thr Glu Cys Gly His Val Phe Cys Ser 
145                 150                 155                 160 


Gln Cys Leu Arg Asp Ser Leu Lys Asn Ala Asn Thr Cys Pro Thr Cys 
                165                 170                 175     


Arg Lys Lys Ile Asn His Lys Arg Tyr His Pro Ile Tyr Ile 
            180                 185                 190 


<210>  2
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  2

Met Ala Ala Gln Gln Arg Asp Cys Gly Gly Ala Ala Gln Leu Ala Gly 
1               5                   10                  15      


Pro Ala Ala Glu Ala Asp Pro Leu Gly Arg Phe Thr Cys Pro Val Cys 
            20                  25                  30          


Leu Glu Val Tyr Glu Lys Pro Val Gln Val Pro Cys Gly His Val Phe 
        35                  40                  45              


Cys Ser Ala Cys Leu Gln Glu Cys Leu Lys Pro Lys Lys Pro Val Cys 
    50                  55                  60                  


Gly Val Cys Arg Ser Ala Leu Ala Pro Gly Val Arg Ala Val Glu Leu 
65                  70                  75                  80  


Glu Arg Gln Ile Glu Ser Thr Glu Thr Ser Cys His Gly Cys Arg Lys 
                85                  90                  95      


Asn Phe Phe Leu Ser Lys Ile Arg Ser His Val Ala Thr Cys Ser Lys 
            100                 105                 110         


Tyr Gln Asn Tyr Ile Met Glu Gly Val Lys Ala Thr Ile Lys Asp Ala 
        115                 120                 125             


Ser Leu Gln Pro Arg Asn Val Pro Asn Arg Tyr Thr Phe Pro Cys Pro 
    130                 135                 140                 


Tyr Cys Pro Glu Lys Asn Phe Asp Gln Glu Gly Leu Val Glu His Cys 
145                 150                 155                 160 


Lys Leu Phe His Ser Thr Asp Thr Lys Ser Val Val Cys Pro Ile Cys 
                165                 170                 175     


Ala Ser Met Pro Trp Gly Asp Pro Asn Tyr Arg Ser Ala Asn Phe Arg 
            180                 185                 190         


Glu His Ile Gln Arg Arg His Arg Phe Ser Tyr Asp Thr Phe Val Asp 
        195                 200                 205             


Tyr Asp Val Asp Glu Glu Asp Met Met Asn Gln Val Leu Gln Arg Ser 
    210                 215                 220                 


Ile Ile Asp Gln 
225             


<210>  3
<211>  1362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  3

Met Ser Ala Glu Ser Gly Pro Gly Thr Arg Leu Arg Asn Leu Pro Val 
1               5                   10                  15      


Met Gly Asp Gly Leu Glu Thr Ser Gln Met Ser Thr Thr Gln Ala Gln 
            20                  25                  30          


Ala Gln Pro Gln Pro Ala Asn Ala Ala Ser Thr Asn Pro Pro Pro Pro 
        35                  40                  45              


Glu Thr Ser Asn Pro Asn Lys Pro Lys Arg Gln Thr Asn Gln Leu Gln 
    50                  55                  60                  


Tyr Leu Leu Arg Val Val Leu Lys Thr Leu Trp Lys His Gln Phe Ala 
65                  70                  75                  80  


Trp Pro Phe Gln Gln Pro Val Asp Ala Val Lys Leu Asn Leu Pro Asp 
                85                  90                  95      


Tyr Tyr Lys Ile Ile Lys Thr Pro Met Asp Met Gly Thr Ile Lys Lys 
            100                 105                 110         


Arg Leu Glu Asn Asn Tyr Tyr Trp Asn Ala Gln Glu Cys Ile Gln Asp 
        115                 120                 125             


Phe Asn Thr Met Phe Thr Asn Cys Tyr Ile Tyr Asn Lys Pro Gly Asp 
    130                 135                 140                 


Asp Ile Val Leu Met Ala Glu Ala Leu Glu Lys Leu Phe Leu Gln Lys 
145                 150                 155                 160 


Ile Asn Glu Leu Pro Thr Glu Glu Thr Glu Ile Met Ile Val Gln Ala 
                165                 170                 175     


Lys Gly Arg Gly Arg Gly Arg Lys Glu Thr Gly Thr Ala Lys Pro Gly 
            180                 185                 190         


Val Ser Thr Val Pro Asn Thr Thr Gln Ala Ser Thr Pro Pro Gln Thr 
        195                 200                 205             


Gln Thr Pro Gln Pro Asn Pro Pro Pro Val Gln Ala Thr Pro His Pro 
    210                 215                 220                 


Phe Pro Ala Val Thr Pro Asp Leu Ile Val Gln Thr Pro Val Met Thr 
225                 230                 235                 240 


Val Val Pro Pro Gln Pro Leu Gln Thr Pro Pro Pro Val Pro Pro Gln 
                245                 250                 255     


Pro Gln Pro Pro Pro Ala Pro Ala Pro Gln Pro Val Gln Ser His Pro 
            260                 265                 270         


Pro Ile Ile Ala Ala Thr Pro Gln Pro Val Lys Thr Lys Lys Gly Val 
        275                 280                 285             


Lys Arg Lys Ala Asp Thr Thr Thr Pro Thr Thr Ile Asp Pro Ile His 
    290                 295                 300                 


Glu Pro Pro Ser Leu Pro Pro Glu Pro Lys Thr Thr Lys Leu Gly Gln 
305                 310                 315                 320 


Arg Arg Glu Ser Ser Arg Pro Val Lys Pro Pro Lys Lys Asp Val Pro 
                325                 330                 335     


Asp Ser Gln Gln His Pro Ala Pro Glu Lys Ser Ser Lys Val Ser Glu 
            340                 345                 350         


Gln Leu Lys Cys Cys Ser Gly Ile Leu Lys Glu Met Phe Ala Lys Lys 
        355                 360                 365             


His Ala Ala Tyr Ala Trp Pro Phe Tyr Lys Pro Val Asp Val Glu Ala 
    370                 375                 380                 


Leu Gly Leu His Asp Tyr Cys Asp Ile Ile Lys His Pro Met Asp Met 
385                 390                 395                 400 


Ser Thr Ile Lys Ser Lys Leu Glu Ala Arg Glu Tyr Arg Asp Ala Gln 
                405                 410                 415     


Glu Phe Gly Ala Asp Val Arg Leu Met Phe Ser Asn Cys Tyr Lys Tyr 
            420                 425                 430         


Asn Pro Pro Asp His Glu Val Val Ala Met Ala Arg Lys Leu Gln Asp 
        435                 440                 445             


Val Phe Glu Met Arg Phe Ala Lys Met Pro Asp Glu Pro Glu Glu Pro 
    450                 455                 460                 


Val Val Ala Val Ser Ser Pro Ala Val Pro Pro Pro Thr Lys Val Val 
465                 470                 475                 480 


Ala Pro Pro Ser Ser Ser Asp Ser Ser Ser Asp Ser Ser Ser Asp Ser 
                485                 490                 495     


Asp Ser Ser Thr Asp Asp Ser Glu Glu Glu Arg Ala Gln Arg Leu Ala 
            500                 505                 510         


Glu Leu Gln Glu Gln Leu Lys Ala Val His Glu Gln Leu Ala Ala Leu 
        515                 520                 525             


Ser Gln Pro Gln Gln Asn Lys Pro Lys Lys Lys Glu Lys Asp Lys Lys 
    530                 535                 540                 


Glu Lys Lys Lys Glu Lys His Lys Arg Lys Glu Glu Val Glu Glu Asn 
545                 550                 555                 560 


Lys Lys Ser Lys Ala Lys Glu Pro Pro Pro Lys Lys Thr Lys Lys Asn 
                565                 570                 575     


Asn Ser Ser Asn Ser Asn Val Ser Lys Lys Glu Pro Ala Pro Met Lys 
            580                 585                 590         


Ser Lys Pro Pro Pro Thr Tyr Glu Ser Glu Glu Glu Asp Lys Cys Lys 
        595                 600                 605             


Pro Met Ser Tyr Glu Glu Lys Arg Gln Leu Ser Leu Asp Ile Asn Lys 
    610                 615                 620                 


Leu Pro Gly Glu Lys Leu Gly Arg Val Val His Ile Ile Gln Ser Arg 
625                 630                 635                 640 


Glu Pro Ser Leu Lys Asn Ser Asn Pro Asp Glu Ile Glu Ile Asp Phe 
                645                 650                 655     


Glu Thr Leu Lys Pro Ser Thr Leu Arg Glu Leu Glu Arg Tyr Val Thr 
            660                 665                 670         


Ser Cys Leu Arg Lys Lys Arg Lys Pro Gln Ala Glu Lys Val Asp Val 
        675                 680                 685             


Ile Ala Gly Ser Ser Lys Met Lys Gly Phe Ser Ser Ser Glu Ser Glu 
    690                 695                 700                 


Ser Ser Ser Glu Ser Ser Ser Ser Asp Ser Glu Asp Ser Glu Thr Glu 
705                 710                 715                 720 


Met Ala Pro Lys Ser Lys Lys Lys Gly His Pro Gly Arg Glu Gln Lys 
                725                 730                 735     


Lys His His His His His His Gln Gln Met Gln Gln Ala Pro Ala Pro 
            740                 745                 750         


Val Pro Gln Gln Pro Pro Pro Pro Pro Gln Gln Pro Pro Pro Pro Pro 
        755                 760                 765             


Pro Pro Gln Gln Gln Gln Gln Pro Pro Pro Pro Pro Pro Pro Pro Ser 
    770                 775                 780                 


Met Pro Gln Gln Ala Ala Pro Ala Met Lys Ser Ser Pro Pro Pro Phe 
785                 790                 795                 800 


Ile Ala Thr Gln Val Pro Val Leu Glu Pro Gln Leu Pro Gly Ser Val 
                805                 810                 815     


Phe Asp Pro Ile Gly His Phe Thr Gln Pro Ile Leu His Leu Pro Gln 
            820                 825                 830         


Pro Glu Leu Pro Pro His Leu Pro Gln Pro Pro Glu His Ser Thr Pro 
        835                 840                 845             


Pro His Leu Asn Gln His Ala Val Val Ser Pro Pro Ala Leu His Asn 
    850                 855                 860                 


Ala Leu Pro Gln Gln Pro Ser Arg Pro Ser Asn Arg Ala Ala Ala Leu 
865                 870                 875                 880 


Pro Pro Lys Pro Ala Arg Pro Pro Ala Val Ser Pro Ala Leu Thr Gln 
                885                 890                 895     


Thr Pro Leu Leu Pro Gln Pro Pro Met Ala Gln Pro Pro Gln Val Leu 
            900                 905                 910         


Leu Glu Asp Glu Glu Pro Pro Ala Pro Pro Leu Thr Ser Met Gln Met 
        915                 920                 925             


Gln Leu Tyr Leu Gln Gln Leu Gln Lys Val Gln Pro Pro Thr Pro Leu 
    930                 935                 940                 


Leu Pro Ser Val Lys Val Gln Ser Gln Pro Pro Pro Pro Leu Pro Pro 
945                 950                 955                 960 


Pro Pro His Pro Ser Val Gln Gln Gln Leu Gln Gln Gln Pro Pro Pro 
                965                 970                 975     


Pro Pro Pro Pro Gln Pro Gln Pro Pro Pro Gln Gln Gln His Gln Pro 
            980                 985                 990         


Pro Pro Arg Pro Val His Leu Gln  Pro Met Gln Phe Ser  Thr His Ile 
        995                 1000                 1005             


Gln Gln  Pro Pro Pro Pro Gln  Gly Gln Gln Pro Pro  His Pro Pro 
    1010                 1015                 1020             


Pro Gly  Gln Gln Pro Pro Pro  Pro Gln Pro Ala Lys  Pro Gln Gln 
    1025                 1030                 1035             


Val Ile  Gln His His His Ser  Pro Arg His His Lys  Ser Asp Pro 
    1040                 1045                 1050             


Tyr Ser  Thr Gly His Leu Arg  Glu Ala Pro Ser Pro  Leu Met Ile 
    1055                 1060                 1065             


His Ser  Pro Gln Met Ser Gln  Phe Gln Ser Leu Thr  His Gln Ser 
    1070                 1075                 1080             


Pro Pro  Gln Gln Asn Val Gln  Pro Lys Lys Gln Glu  Leu Arg Ala 
    1085                 1090                 1095             


Ala Ser  Val Val Gln Pro Gln  Pro Leu Val Val Val  Lys Glu Glu 
    1100                 1105                 1110             


Lys Ile  His Ser Pro Ile Ile  Arg Ser Glu Pro Phe  Ser Pro Ser 
    1115                 1120                 1125             


Leu Arg  Pro Glu Pro Pro Lys  His Pro Glu Ser Ile  Lys Ala Pro 
    1130                 1135                 1140             


Val His  Leu Pro Gln Arg Pro  Glu Met Lys Pro Val  Asp Val Gly 
    1145                 1150                 1155             


Arg Pro  Val Ile Arg Pro Pro  Glu Gln Asn Ala Pro  Pro Pro Gly 
    1160                 1165                 1170             


Ala Pro  Asp Lys Asp Lys Gln  Lys Gln Glu Pro Lys  Thr Pro Val 
    1175                 1180                 1185             


Ala Pro  Lys Lys Asp Leu Lys  Ile Lys Asn Met Gly  Ser Trp Ala 
    1190                 1195                 1200             


Ser Leu  Val Gln Lys His Pro  Thr Thr Pro Ser Ser  Thr Ala Lys 
    1205                 1210                 1215             


Ser Ser  Ser Asp Ser Phe Glu  Gln Phe Arg Arg Ala  Ala Arg Glu 
    1220                 1225                 1230             


Lys Glu  Glu Arg Glu Lys Ala  Leu Lys Ala Gln Ala  Glu His Ala 
    1235                 1240                 1245             


Glu Lys  Glu Lys Glu Arg Leu  Arg Gln Glu Arg Met  Arg Ser Arg 
    1250                 1255                 1260             


Glu Asp  Glu Asp Ala Leu Glu  Gln Ala Arg Arg Ala  His Glu Glu 
    1265                 1270                 1275             


Ala Arg  Arg Arg Gln Glu Gln  Gln Gln Gln Gln Arg  Gln Glu Gln 
    1280                 1285                 1290             


Gln Gln  Gln Gln Gln Gln Gln  Ala Ala Ala Val Ala  Ala Ala Ala 
    1295                 1300                 1305             


Thr Pro  Gln Ala Gln Ser Ser  Gln Pro Gln Ser Met  Leu Asp Gln 
    1310                 1315                 1320             


Gln Arg  Glu Leu Ala Arg Lys  Arg Glu Gln Glu Arg  Arg Arg Arg 
    1325                 1330                 1335             


Glu Ala  Met Ala Ala Thr Ile  Asp Met Asn Phe Gln  Ser Asp Leu 
    1340                 1345                 1350             


Leu Ser  Ile Phe Glu Glu Asn  Leu Phe 
    1355                 1360         


<210>  4
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  4
ccggccatgg ctgccgtaag aatttctcga gaaattctta cggcagccat ggtttttg         58


<210>  5
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  5
gcaagctgac cctgaagttc at                                                22


<210>  6
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  6
aatgttccaa accg                                                         14


<210>  7
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  7
ttgcagtgtt ccac                                                         14


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  8
cccaccgtgt tcttcgacat t                                                 21


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  9
ggacccgtat gctttaggat ga                                                22


<210>  10
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  10
gugugaaggc caccauuaa                                                    19


<210>  11
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  11
gcuuagaggu guacgagaa                                                    19


<210>  12
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  12
gcacggauac caaaucugu                                                    19


<210>  13
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  13
agaccagcau gacagauuu                                                    19


<210>  14
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  14
cugagaaguc gucgggcga                                                    19


<210>  15
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  15
tgtccgtcag aacccatgc                                                    19


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  16
aaagtcgaag ttccatcgct c                                                 21


