                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  Methods and reagents for analysing nucleic acids from single 
       cells

<130>  P115660PCT

<140>  PCT/GB2019/052882
<141>  2019-10-10

<150>  GB 1816522.5
<151>  2018-10-10

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like cleavage site sequence

<400>  1

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  2
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide

<400>  2

Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Asp His Ala Asp Gly 
            20      


<210>  3
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from IgG1

<400>  3

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  4
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from CD8

<400>  4

Met Ala Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys 
            20  


<210>  5
<211>  7
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Kozak consensus sequence

<400>  5
accaugg                                                                  7


<210>  6
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  labelling sequence

<400>  6
gctggcacta cgaca                                                        15


<210>  7
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  labelling sequence

<400>  7
gacattatct ttcgc                                                        15


<210>  8
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  labelling sequence

<400>  8
cattttacct acctg                                                        15


<210>  9
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  labelling sequence

<400>  9
cacaatattg ttggg                                                        15


<210>  10
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  labelling sequence

<400>  10
attgccttgg catct                                                        15


<210>  11
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  labelling sequence

<400>  11
cgattctagt gacga                                                        15


<210>  12
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  labelling sequence

<400>  12
caagacaaac gatgc                                                        15


<210>  13
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  labelling sequence

<400>  13
caactacagt ttcac                                                        15


<210>  14
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  labelling sequence

<400>  14
gcgctagtct ccaca                                                        15


<210>  15
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  labelling sequence

<400>  15
tccactatcg ttcaa                                                        15


<210>  16
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polyadenylation signal sequence

<400>  16
aauaaa                                                                   6


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer

<400>  17
acagcagcag ggtgtcggtc t                                                 21


<210>  18
<211>  601
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' RACE_Vector 16248

<400>  18
gcgccagtcc tccgacagac tgagtcgccc ggggtcgacg ccaccatgct gaccagcctg       60

ctgtgctgga tggccctgtg cctgctgggc gccgaccacg ccgatgcctg cccctacagc      120

aaccccagcc tgtgcagcgg aggcggcggc agcgagctgc ccacccaggg caccttctcc      180

aacgtgtcca ccaacgtgag cccagccaag cccaccacca ccgcctgtcc ttattccaat      240

ccttccctgt gtagcggagg gggaggcagc ccagccccca gacctcccac cccagccccc      300

accatcgcca gccagcctct gagcctgaga cccgaggcct gccgcccagc cgccggcggc      360

gccgtgcaca ccagaggcct ggatttcgcc tgcgatatct acatctgggc cccactggcc      420

ggcacctgtg gcgtgctgct gctgagcctg gtgatcaccc tgtactgcaa ccaccgcaac      480

cgcaggcgcg tgtgcaagtg ccccaggccc gtggtgagag ccgagggcag aggcagcctg      540

ctgacctgcg gcgacgtgga ggagaaccca ggccccatgg agaccgacac cctgctgctg      600

t                                                                      601


<210>  19
<211>  566
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' RACE_Vector 46635

<400>  19
gcgccagtcc tccgacgctg gcactacgac aagactgagt cgcccggggt cgacgccacc       60

atgctgacca gcctgctgtg ctggatggcc ctgtgcctgc tgggcgccga ccacgccgat      120

gcctgcccct acagcaaccc cagcctgtgc agcggaggcg gcggcagcga gctgcccacc      180

cagggcacct tctccaacgt gtccaccaac gtgagcccag ccaagcccac caccaccgcc      240

tgtccttatt ccaatccttc cctgtgtagc ggagggggag gcagcccagc ccccagacct      300

cccaccccag cccccaccat cgccagccag cctctgagcc tgagacccga ggcctgccgc      360

ccagccgccg gcggcgccgt gcacaccaga ggcctggatt tcgcctgcga tatctacatc      420

tgggccccac tggccggcac ctgtggcgtg ctgctgctga gcctggtgat caccctgtac      480

tgcaaccacc gcaaccgcag gcgcgtgtgc aagtgcccca ggcccgtggt gagagccgag      540

ggcagaggca gcctgctgac ctgcgg                                           566


<210>  20
<211>  569
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' RACE_Vector 46636

<400>  20
gcgccagtcc tccgacagac tggctggcac tacgacaagt cgcccggggt cgacgccacc       60

atgctgacca gcctgctgtg ctggatggcc ctgtgcctgc tgggcgccga ccacgccgat      120

gcctgcccct acagcaaccc cagcctgtgc agcggaggcg gcggcagcga gctgcccacc      180

cagggcacct tctccaacgt gtccaccaac gtgagcccag ccaagcccac caccaccgcc      240

tgtccttatt ccaatccttc cctgtgtagc ggagggggag gcagcccagc ccccagacct      300

cccaccccag cccccaccat cgccagccag cctctgagcc tgagacccga ggcctgccgc      360

ccagccgccg gcggcgccgt gcacaccaga ggcctggatt tcgcctgcga tatctacatc      420

tgggccccac tggccggcac ctgtggcgtg ctgctgctga gcctggtgat caccctgtac      480

tgcaaccacc gcaaccgcag gcgcgtgtgc aagtgcccca ggcccgtggt gagagccgag      540

ggcagaggca gcctgctgac ctgcggcga                                        569


<210>  21
<211>  616
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' RACE_Vector 46637

<400>  21
gcgccagtcc tccgacagac tgagtcgcgc tggcactacg acaccggggt cgacgccacc       60

atgctgacca gcctgctgtg ctggatggcc ctgtgcctgc tgggcgccga ccacgccgat      120

gcctgcccct acagcaaccc cagcctgtgc agcggaggcg gcggcagcga gctgcccacc      180

cagggcacct tctccaacgt gtccaccaac gtgagcccag ccaagcccac caccaccgcc      240

tgtccttatt ccaatccttc cctgtgtagc ggagggggag gcagcccagc ccccagacct      300

cccaccccag cccccaccat cgccagccag cctctgagcc tgagacccga ggcctgccgc      360

ccagccgccg gcggcgccgt gcacaccaga ggcctggatt tcgcctgcga tatctacatc      420

tgggccccac tggccggcac ctgtggcgtg ctgctgctga gcctggtgat caccctgtac      480

tgcaaccacc gcaaccgcag gcgcgtgtgc aagtgcccca ggcccgtggt gagagccgag      540

ggcagaggca gcctgctgac ctgcggcgac gtggaggaga acccaggccc catggagacc      600

gacaccctgc tgctgt                                                      616


<210>  22
<211>  570
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' RACE_Vector 46638

<400>  22
gcgccagtcc tccgacagac tgagtcgccc gggggctggc actacgacat cgacgccacc       60

atgctgacca gcctgctgtg ctggatggcc ctgtgcctgc tgggcgccga ccacgccgat      120

gcctgcccct acagcaaccc cagcctgtgc agcggaggcg gcggcagcga gctgcccacc      180

cagggcacct tctccaacgt gtccaccaac gtgagcccag ccaagcccac caccaccgcc      240

tgtccttatt ccaatccttc cctgtgtagc ggagggggag gcagcccagc ccccagacct      300

cccaccccag cccccaccat cgccagccag cctctgagcc tgagacccga ggcctgccgc      360

ccagccgccg gcggcgccgt gcacaccaga ggcctggatt tcgcctgcga tatctacatc      420

tgggccccac tggccggcac ctgtggcgtg ctgctgctga gcctggtgat caccctgtac      480

tgcaaccacc gcaaccgcag gcgcgtgtgc aagtgcccca ggcccgtggt gagagccgag      540

ggcagaggca gcctgctgac ctgcggcgac                                       570


