                         SEQUENCE LISTING

<110>  Queen Mary University of London
 
<120>  Oncolytic Virus for the Treatment of Cancer

<130>  P118869GB

<160>  69    

<170>  PatentIn version 3.5

<210>  1
<211>  95
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  1
agatctaaaa attgaaaata aatacaaagg ttcttgaggg ttgtgttaaa ttgaaagcga       60

gaaataatca taaatagcta ccggactcag atcca                                  95


<210>  2
<211>  110
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  2
acgcgtcccg ggaagcttta tttatgatta tttctcgctt tcaatttaac acaaccctca       60

agaacctttg tatttatttt caatttttcg ccttaagata cattgatgag                 110


<210>  3
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  3
aagcttaaat aaaaatgaaa acgatttcc                                         29


<210>  4
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  4
cccggggaat tcagatcttt ttatttatga gcgttaaaaa tagtata                     47


<210>  5
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  5
tatactgcgt gtatgaccg                                                    19


<210>  6
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  6
cccggggaat tcagatcttt ttatttatga gcgttaaaaa tagtata                     47


<210>  7
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  7
tatactgcgt gtatgaccg                                                    19


<210>  8
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  8
ctcgaggaat tcaagcttgc atggattttc gtatttc                                37


<210>  9
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  9
aagctttgtg tacgaactaa cgaaaaa                                           27


<210>  10
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  10
agatcttcac ggtagcaatt tatgg                                             25


<210>  11
<211>  78
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  11
agatctaaaa attgaaaata aatacaaagg ttcttgaggg ttgtgttaaa ttgaaagcga       60

gaaataatca taaatagc                                                     78


<210>  12
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  12
acgcgtcgcc ttaagataca ttgatgag                                          28


<210>  13
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  13
acgcgtctac cgtgaatata aatccgt                                           27


<210>  14
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  14
ctcgagggat gtatatacca tcgtcgt                                           27


<210>  15
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  15
ttaattaaaa ataaaaatga aaacgatttc cg                                     32


<210>  16
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  16
gctagcgaat tcaagctttg aataaacaac agc                                    33


<210>  17
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  17
aagctttgtg tacgaactaa cgaaaaa                                           27


<210>  18
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  18
gctagctcac ggtagcaatt tatggaact                                         29


<210>  19
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  19
aaataaaaat gaaaacgatt tccg                                              24


<210>  20
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  20
ggatgtatat accatcgtcg t                                                 21


<210>  21
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  21
ttggctatta aacagtatgg a                                                 21


<210>  22
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  22
ggatcccgat aacaaatg                                                     18


<210>  23
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  23
tatctagcaa tggaccgt                                                     18


<210>  24
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  24
ccgaaggtag tagcatgga                                                    19


<210>  25
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer

<400>  25
ttggctatta aacagtatgg a                                                 21


<210>  26
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer

<400>  26
ggatcccgat aacaaatg                                                     18


<210>  27
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  signal peptide of B5R-S-STC (amino acid residues 1-19)

<400>  27

Met Lys Thr Ile Ser Val Val Thr Leu Leu Cys Val Leu Pro Ala Val 
1               5                   10                  15      


Val Tyr Ser Thr 
            20  


<210>  28
<211>  57
<212>  DNA
<213>  Artificial

<220>
<223>  signal peptide of B5R-S-STC

<400>  28
atgaaaacga tttccgttgt tacgttgtta tgcgtactac ctgctgttgt ttattca          57


<210>  29
<211>  52
<212>  PRT
<213>  Artificial

<220>
<223>  SCR1 domain of B5R-S-STC (amino acids 20-72)

<400>  29

Thr Cys Thr Val Pro Thr Met Asn Asn Ala Lys Leu Thr Ser Thr Glu 
1               5                   10                  15      


Thr Ser Phe Asn Asp Lys Gln Lys Val Thr Phe Thr Cys Asp Gln Gly 
            20                  25                  30          


Tyr His Ser Ser Asp Pro Asn Ala Val Cys Glu Thr Asp Lys Trp Lys 
        35                  40                  45              


Tyr Glu Asn Pro 
    50          


<210>  30
<211>  153
<212>  DNA
<213>  Artificial

<220>
<223>  SCR1 domain of B5R-S-STC

<400>  30
acaactgtac ccactatgaa taacgctaaa ttaacgtcta ccgaaacatc gtttaatgat       60

aaacagaaag ttacatttac atgtgatcag ggatatcatt cttcggatcc aaatgctgtc      120

tgcgaaacag ataaatggaa atacgaaaat cca                                   153


<210>  31
<211>  39
<212>  PRT
<213>  Artificial

<220>
<223>  STALK domain of B5R-S-STC (amino acids 237-275)

<400>  31

Cys Val Arg Thr Asn Glu Lys Phe Asp Pro Val Asp Asp Gly Pro Asp 
1               5                   10                  15      


Asp Glu Thr Asp Leu Ser Lys Leu Ser Lys Asp Val Val Gln Tyr Glu 
            20                  25                  30          


Gln Glu Ile Glu Ser Leu Glu 
        35                  


<210>  32
<211>  117
<212>  DNA
<213>  Artificial

<220>
<223>  STALK domain of B5R-S-STC

<400>  32
tgtgtacgaa ctaacgaaaa atttgatcca gtggatgatg gtcccgacga tgagacagat       60

ttgagcaaac tctcgaaaga cgttgtacaa tatgaacaag aaatagaatc gttagaa         117


<210>  33
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  transmembrane domain of B5R-S-STC (amino acids 276-303)

<400>  33

Ala Thr Tyr His Ile Ile Ile Val Ala Leu Thr Ile Met Gly Val Ile 
1               5                   10                  15      


Phe Leu Ile 
            


<210>  34
<211>  57
<212>  DNA
<213>  Artificial

<220>
<223>  transmembrane domain of B5R-S-STC

<400>  34
gcaacttatc atataatcat agtggcgttg acaattatgg gcgtcatatt tttaatc          57


<210>  35
<211>  23
<212>  PRT
<213>  Artificial

<220>
<223>  C terminal domain of B5R-S-STC (amino acids 304-317)

<400>  35

Ser Val Ile Val Leu Val Cys Ser Cys Asp Lys Asn Asn Asp Gln Tyr 
1               5                   10                  15      


Lys Phe His Lys Leu Leu Pro 
            20              


<210>  36
<211>  72
<212>  DNA
<213>  Artificial

<220>
<223>  C terminal domain of B5R-S-STC

<400>  36
tccgttatag tattagtttg ttcctgtgac aaaaataatg accaatataa gttccataaa       60

ttgctaccgt ga                                                           72


<210>  37
<211>  70
<212>  DNA
<213>  Artificial

<220>
<223>  H5 promoter of B5R-S-STC

<400>  37
aaaaattgaa aataaataca aaggttcttg agggttgtgt taaattgaaa gcgagaaata       60

atcataaata                                                              70


<210>  38
<211>  225
<212>  PRT
<213>  Artificial

<220>
<223>  Red Fluorescent Protein (RFP) of B5R-S-STC (amino acids 1-255)

<400>  38

Met Ala Ser Ser Glu Asn Val Ile Thr Glu Phe Met Arg Phe Lys Val 
1               5                   10                  15      


Arg Met Glu Gly Thr Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu 
            20                  25                  30          


Gly Glu Gly Arg Pro Tyr Glu Gly His Asn Thr Val Lys Leu Lys Val 
        35                  40                  45              


Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln 
    50                  55                  60                  


Phe Gln Tyr Gly Ser Lys Val Tyr Val Lys His Pro Ala Asp Ile Pro 
65                  70                  75                  80  


Asp Tyr Lys Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val 
                85                  90                  95      


Met Asn Phe Glu Asp Gly Gly Val Ala Thr Val Thr Gln Asp Ser Ser 
            100                 105                 110         


Leu Gln Asp Gly Cys Phe Ile Tyr Lys Val Lys Phe Ile Gly Val Asn 
        115                 120                 125             


Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu 
    130                 135                 140                 


Ala Ser Thr Glu Arg Leu Tyr Pro Arg Asp Gly Val Leu Lys Gly Glu 
145                 150                 155                 160 


Thr His Lys Ala Leu Lys Leu Lys Asp Gly Gly His Tyr Leu Val Glu 
                165                 170                 175     


Phe Lys Ser Ile Tyr Met Ala Lys Lys Pro Val Gln Leu Pro Gly Tyr 
            180                 185                 190         


Tyr Tyr Val Asp Ala Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr 
        195                 200                 205             


Thr Ile Val Glu Gln Tyr Glu Arg Thr Glu Gly Arg His His Leu Phe 
    210                 215                 220                 


Leu 
225 


<210>  39
<211>  678
<212>  DNA
<213>  Artificial

<220>
<223>  nucleic acid sequence of Red Fluorescent Protein (RFP) of 
       B5R-S-STC

<400>  39
atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg catggagggc       60

accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc      120

cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc      180

ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc      240

gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag      300

gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg cttcatctac      360

aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca gaagaagacc      420

atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag      480

acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc      540

tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc caagctggac      600

atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac cgagggccgc      660

caccacctgt tcctgtag                                                    678


<210>  40
<211>  153
<212>  PRT
<213>  Artificial

<220>
<223>  SP(aa 1-19)-SCR1(aa 20-72)-STALK(aa 237-275)-TM(aa 276-303)- 
       CT(aa 304-317)

<400>  40

Met Lys Thr Ile Ser Val Val Thr Leu Leu Cys Val Leu Pro Ala Val 
1               5                   10                  15      


Val Tyr Ser Thr Thr Cys Thr Val Pro Thr Met Asn Asn Ala Lys Leu 
            20                  25                  30          


Thr Ser Thr Glu Thr Ser Phe Asn Asp Lys Gln Lys Val Thr Phe Thr 
        35                  40                  45              


Cys Asp Gln Gly Tyr His Ser Ser Asp Pro Asn Ala Val Cys Glu Thr 
    50                  55                  60                  


Asp Lys Trp Lys Tyr Glu Asn Pro Cys Val Arg Thr Asn Glu Lys Phe 
65                  70                  75                  80  


Asp Pro Val Asp Asp Gly Pro Asp Asp Glu Thr Asp Leu Ser Lys Leu 
                85                  90                  95      


Ser Lys Asp Val Val Gln Tyr Glu Gln Glu Ile Glu Ser Leu Glu Ala 
            100                 105                 110         


Thr Tyr His Ile Ile Ile Val Ala Leu Thr Ile Met Gly Val Ile Phe 
        115                 120                 125             


Leu Ile Ser Val Ile Val Leu Val Cys Ser Cys Asp Lys Asn Asn Asp 
    130                 135                 140                 


Gln Tyr Lys Phe His Lys Leu Leu Pro 
145                 150             


<210>  41
<211>  456
<212>  DNA
<213>  Artificial

<220>
<223>  SP(aa 1-19)-SCR1(aa 20-72)-STALK(aa 237-275)-TM(aa 276-303)- 
       CT(aa 304-317)

<400>  41
atgaaaacga tttccgttgt tacgttgtta tgcgtactac ctgctgttgt ttattcaaca       60

actgtaccca ctatgaataa cgctaaatta acgtctaccg aaacatcgtt taatgataaa      120

cagaaagtta catttacatg tgatcaggga tatcattctt cggatccaaa tgctgtctgc      180

gaaacagata aatggaaata cgaaaatcca tgtgtacgaa ctaacgaaaa atttgatcca      240

gtggatgatg gtcccgacga tgagacagat ttgagcaaac tctcgaaaga cgttgtacaa      300

tatgaacaag aaatagaatc gttagaagca acttatcata taatcatagt ggcgttgaca      360

attatgggcg tcatattttt aatctccgtt atagtattag tttgttcctg tgacaaaaat      420

aatgaccaat ataagttcca taaattgcta ccgtga                                456


<210>  42
<211>  70
<212>  DNA
<213>  Artificial

<220>
<223>  H5 promoter of B5R-STC

<400>  42
aaaaattgaa aataaataca aaggttcttg agggttgtgt taaattgaaa gcgagaaata       60

atcataaata                                                              70


<210>  43
<211>  225
<212>  PRT
<213>  Artificial

<220>
<223>  Red Fluorescent Protein (RFP) of B5R-STC (amino acids 1-225)

<400>  43

Met Ala Ser Ser Glu Asn Val Ile Thr Glu Phe Met Arg Phe Lys Val 
1               5                   10                  15      


Arg Met Glu Gly Thr Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu 
            20                  25                  30          


Gly Glu Gly Arg Pro Tyr Glu Gly His Asn Thr Val Lys Leu Lys Val 
        35                  40                  45              


Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln 
    50                  55                  60                  


Phe Gln Tyr Gly Ser Lys Val Tyr Val Lys His Pro Ala Asp Ile Pro 
65                  70                  75                  80  


Asp Tyr Lys Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val 
                85                  90                  95      


Met Asn Phe Glu Asp Gly Gly Val Ala Thr Val Thr Gln Asp Ser Ser 
            100                 105                 110         


Leu Gln Asp Gly Cys Phe Ile Tyr Lys Val Lys Phe Ile Gly Val Asn 
        115                 120                 125             


Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu 
    130                 135                 140                 


Ala Ser Thr Glu Arg Leu Tyr Pro Arg Asp Gly Val Leu Lys Gly Glu 
145                 150                 155                 160 


Thr His Lys Ala Leu Lys Leu Lys Asp Gly Gly His Tyr Leu Val Glu 
                165                 170                 175     


Phe Lys Ser Ile Tyr Met Ala Lys Lys Pro Val Gln Leu Pro Gly Tyr 
            180                 185                 190         


Tyr Tyr Val Asp Ala Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr 
        195                 200                 205             


Thr Ile Val Glu Gln Tyr Glu Arg Thr Glu Gly Arg His His Leu Phe 
    210                 215                 220                 


Leu 
225 


<210>  44
<211>  678
<212>  DNA
<213>  Artificial

<220>
<223>  Red Fluorescent Protein (RFP) of B5R-STC

<400>  44
atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg catggagggc       60

accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc      120

cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc      180

ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc      240

gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag      300

gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg cttcatctac      360

aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca gaagaagacc      420

atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag      480

acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc      540

tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc caagctggac      600

atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac cgagggccgc      660

caccacctgt tcctgtag                                                    678


<210>  45
<211>  70
<212>  DNA
<213>  Artificial

<220>
<223>  H5 promoter

<400>  45
aaaaattgaa aataaataca aaggttcttg agggttgtgt taaattgaaa gcgagaaata       60

atcataaata                                                              70


<210>  46
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  signal peptide of B5R-STC (amino acids 1-19)

<400>  46

Met Lys Thr Ile Ser Val Val Thr Leu Leu Cys Val Leu Pro Ala Val 
1               5                   10                  15      


Val Tyr Ser Thr 
            20  


<210>  47
<211>  57
<212>  DNA
<213>  Artificial

<220>
<223>  signal peptide of B5R-STC

<400>  47
atgaaaacga tttccgttgt tacgttgtta tgcgtactac ctgctgttgt ttattca          57


<210>  48
<211>  39
<212>  PRT
<213>  Artificial

<220>
<223>  STALK of B5R-STC (amino acids 237-275)

<400>  48

Cys Val Arg Thr Asn Glu Lys Phe Asp Pro Val Asp Asp Gly Pro Asp 
1               5                   10                  15      


Asp Glu Thr Asp Leu Ser Lys Leu Ser Lys Asp Val Val Gln Tyr Glu 
            20                  25                  30          


Gln Glu Ile Glu Ser Leu Glu 
        35                  


<210>  49
<211>  117
<212>  DNA
<213>  Artificial

<220>
<223>  STALK of B5R-STC

<400>  49
tgtgtacgaa ctaacgaaaa atttgatcca gtggatgatg gtcccgacga tgagacagat       60

ttgagcaaac tctcgaaaga cgttgtacaa tatgaacaag aaatagaatc gttagaa         117


<210>  50
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  transmembrane domain of B5R-STC (amino acids 276-303)

<400>  50

Ala Thr Tyr His Ile Ile Ile Val Ala Leu Thr Ile Met Gly Val Ile 
1               5                   10                  15      


Phe Leu Ile 
            


<210>  51
<211>  57
<212>  DNA
<213>  Artificial

<220>
<223>  transmembrane domain of B5R-STC

<400>  51
gcaacttatc atataatcat agtggcgttg acaattatgg gcgtcatatt tttaatc          57


<210>  52
<211>  23
<212>  PRT
<213>  Artificial

<220>
<223>  C terminal domain of B5R-STC (amino acids 304-317)

<400>  52

Ser Val Ile Val Leu Val Cys Ser Cys Asp Lys Asn Asn Asp Gln Tyr 
1               5                   10                  15      


Lys Phe His Lys Leu Leu Pro 
            20              


<210>  53
<211>  72
<212>  DNA
<213>  Artificial

<220>
<223>  C terminal domain of B5R-STC

<400>  53
tccgttatag tattagtttg ttcctgtgac aaaaataatg accaatataa gttccataaa       60

ttgctaccgt ga                                                           72


<210>  54
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Loxp elements of TK-STC

<400>  54
acttcgtata gcatacatta tacgaagtta t                                      31


<210>  55
<211>  70
<212>  DNA
<213>  Artificial

<220>
<223>  H5 promoter of TK-STC

<400>  55
aaaaattgaa aataaataca aaggttcttg agggttgtgt taaattgaaa gcgagaaata       60

atcataaata                                                              70


<210>  56
<211>  225
<212>  PRT
<213>  Artificial

<220>
<223>  Red Fluorescent Protein (RFP) of TK-STC (amino acids 1-225)

<400>  56

Met Ala Ser Ser Glu Asn Val Ile Thr Glu Phe Met Arg Phe Lys Val 
1               5                   10                  15      


Arg Met Glu Gly Thr Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu 
            20                  25                  30          


Gly Glu Gly Arg Pro Tyr Glu Gly His Asn Thr Val Lys Leu Lys Val 
        35                  40                  45              


Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln 
    50                  55                  60                  


Phe Gln Tyr Gly Ser Lys Val Tyr Val Lys His Pro Ala Asp Ile Pro 
65                  70                  75                  80  


Asp Tyr Lys Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val 
                85                  90                  95      


Met Asn Phe Glu Asp Gly Gly Val Ala Thr Val Thr Gln Asp Ser Ser 
            100                 105                 110         


Leu Gln Asp Gly Cys Phe Ile Tyr Lys Val Lys Phe Ile Gly Val Asn 
        115                 120                 125             


Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu 
    130                 135                 140                 


Ala Ser Thr Glu Arg Leu Tyr Pro Arg Asp Gly Val Leu Lys Gly Glu 
145                 150                 155                 160 


Thr His Lys Ala Leu Lys Leu Lys Asp Gly Gly His Tyr Leu Val Glu 
                165                 170                 175     


Phe Lys Ser Ile Tyr Met Ala Lys Lys Pro Val Gln Leu Pro Gly Tyr 
            180                 185                 190         


Tyr Tyr Val Asp Ala Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr 
        195                 200                 205             


Thr Ile Val Glu Gln Tyr Glu Arg Thr Glu Gly Arg His His Leu Phe 
    210                 215                 220                 


Leu 
225 


<210>  57
<211>  678
<212>  DNA
<213>  Artificial

<220>
<223>  Red Fluorescent Protein (RFP) of TK-STC

<400>  57
atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg catggagggc       60

accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc      120

cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc      180

ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc      240

gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag      300

gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg cttcatctac      360

aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca gaagaagacc      420

atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag      480

acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc      540

tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc caagctggac      600

atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac cgagggccgc      660

caccacctgt tcctgtag                                                    678


<210>  58
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Loxp of TK-STC

<400>  58
acttcgtata gcatacatta tacgaagtta t                                      31


<210>  59
<211>  70
<212>  DNA
<213>  Artificial

<220>
<223>  H5 promoter of TK-STC

<400>  59
aaaaattgaa aataaataca aaggttcttg agggttgtgt taaattgaaa gcgagaaata       60

atcataaata                                                              70


<210>  60
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  signal peptide of TK-STC (amino acids 1-19)

<400>  60

Met Lys Thr Ile Ser Val Val Thr Leu Leu Cys Val Leu Pro Ala Val 
1               5                   10                  15      


Val Tyr Ser Thr 
            20  


<210>  61
<211>  57
<212>  DNA
<213>  Artificial

<220>
<223>  signal peptide of TK-STC

<400>  61
atgaaaacga tttccgttgt tacgttgtta tgcgtactac ctgctgttgt ttattca          57


<210>  62
<211>  39
<212>  PRT
<213>  Artificial

<220>
<223>  STALK domain of TK-STC (amino acids 237-275)

<400>  62

Cys Val Arg Thr Asn Glu Lys Phe Asp Pro Val Asp Asp Gly Pro Asp 
1               5                   10                  15      


Asp Glu Thr Asp Leu Ser Lys Leu Ser Lys Asp Val Val Gln Tyr Glu 
            20                  25                  30          


Gln Glu Ile Glu Ser Leu Glu 
        35                  


<210>  63
<211>  117
<212>  DNA
<213>  Artificial

<220>
<223>  STALK domain of TK-STC

<400>  63
tgtgtacgaa ctaacgaaaa atttgatcca gtggatgatg gtcccgacga tgagacagat       60

ttgagcaaac tctcgaaaga cgttgtacaa tatgaacaag aaatagaatc gttagaa         117


<210>  64
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  transmembrane domain of TK-STC (amino acids 276-303)

<400>  64

Ala Thr Tyr His Ile Ile Ile Val Ala Leu Thr Ile Met Gly Val Ile 
1               5                   10                  15      


Phe Leu Ile 
            


<210>  65
<211>  57
<212>  DNA
<213>  Artificial

<220>
<223>  transmembrane domain of TK-STC

<400>  65
gcaacttatc atataatcat agtggcgttg acaattatgg gcgtcatatt tttaatc          57


<210>  66
<211>  23
<212>  PRT
<213>  Artificial

<220>
<223>  C terminal domain of TK-STC (amino acids 304-317)

<400>  66

Ser Val Ile Val Leu Val Cys Ser Cys Asp Lys Asn Asn Asp Gln Tyr 
1               5                   10                  15      


Lys Phe His Lys Leu Leu Pro 
            20              


<210>  67
<211>  72
<212>  DNA
<213>  Artificial

<220>
<223>  C terminal domain of TK-STC

<400>  67
tccgttatag tattagtttg ttcctgtgac aaaaataatg accaatataa gttccataaa       60

ttgctaccgt ga                                                           72


<210>  68
<211>  246
<212>  DNA
<213>  Artificial

<220>
<223>  STC construct

<400>  68
tgtgtacgaa ctaacgaaaa atttgatcca gtggatgatg gtcccgacga tgagacagat       60

ttgagcaaac tctcgaaaga cgttgtacaa tatgaacaag aaatagaatc gttagaagca      120

acttatcata taatcatagt ggcgttgaca attatgggcg tcatattttt aatctccgtt      180

atagtattag tttgttcctg tgacaaaaat aatgaccaat ataagttcca taaattgcta      240

ccgtga                                                                 246


<210>  69
<211>  84
<212>  PRT
<213>  Artificial

<220>
<223>  STC construct

<400>  69

Cys Val Arg Thr Asn Glu Lys Phe Asp Pro Val Asp Asp Gly Pro Asp 
1               5                   10                  15      


Asp Glu Thr Asp Leu Ser Lys Leu Ser Lys Asp Val Val Gln Tyr Glu 
            20                  25                  30          


Gln Glu Ile Glu Ser Leu Glu Ser Leu Glu Ala Thr Tyr His Ile Ile 
        35                  40                  45              


Ile Val Ala Leu Thr Ile Met Gly Val Ile Phe Leu Ile Ser Val Ile 
    50                  55                  60                  


Val Leu Val Cys Ser Cys Asp Lys Asn Asn Asp Gln Tyr Lys Phe His 
65                  70                  75                  80  


Lys Leu Leu Pro 
                



