﻿                         序列表

<110>  中国农业科学院饲料研究所

<120>  葡萄糖淀粉酶15及其基因和应用

<160>  9

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  613
<212>  PRT
<213>  嗜热蓝状菌JCM 12800(Talaromyces leycettanus JCM 12802)

<400>  1
Met Gln Tyr Leu Leu Lys Thr Thr Leu Gly Ala Leu Ser Val Ala Gln 
1               5                   10                  15      
Leu Val Ile Ala Ala Pro His Pro Thr Glu Leu Leu Pro Arg Ala Ser 
            20                  25                  30          
Gly Ser Leu Asp Ser Trp Leu Ser Thr Glu Val Pro Tyr Ala Leu Asp 
        35                  40                  45              
Gly Val Leu Asn Asn Ile Gly Pro Asn Gly Ala Lys Ala Gln Gly Ala 
    50                  55                  60                  
Ser Ser Gly Ile Val Val Ala Ser Pro Ser Thr Ser Asn Pro Asp Tyr 
65                  70                  75                  80  
Phe Tyr Ser Trp Thr Arg Asp Ala Ala Leu Thr Ile Lys Cys Leu Ile 
                85                  90                  95      
Asp Glu Phe Ile Ser Thr Gly Asp Ala Asn Leu Gln Ser Val Ile Gln 
            100                 105                 110         
Asn Tyr Ile Ser Ser Gln Ala Phe Leu Gln Thr Val Ser Asn Pro Ser 
        115                 120                 125             
Gly Gly Leu Ser Thr Gly Gly Leu Gly Glu Pro Lys Phe Glu Val Asn 
    130                 135                 140                 
Glu Ala Ala Phe Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro 
145                 150                 155                 160 
Ala Leu Arg Ala Thr Ala Met Ile Asn Tyr Ala Asn Trp Leu Ile Ala 
                165                 170                 175     
Asn Gly Gln Ala Ser Leu Ala Asn Ser Ile Val Trp Pro Ile Val Gln 
            180                 185                 190         
Asn Asp Leu Ser Tyr Val Ser Gln Tyr Trp Asn Gln Ser Thr Phe Asp 
        195                 200                 205             
Leu Trp Glu Glu Ile Asp Ser Ser Ser Phe Phe Thr Thr Ala Val Gln 
    210                 215                 220                 
His Arg Ala Leu Val Glu Gly Ser Ala Leu Ala Lys Lys Leu Gly His 
225                 230                 235                 240 
Thr Cys Ser Asn Cys Asp Ser Gln Ala Pro Leu Val Leu Cys Phe Leu 
                245                 250                 255     
Gln Ser Tyr Trp Thr Gly Ser Tyr Ile Leu Ser Asn Thr Gly Gly Gly 
            260                 265                 270         
Arg Ser Gly Lys Asp Ala Asn Ser Leu Leu Gly Ser Ile His Thr Phe 
        275                 280                 285             
Asp Pro Ala Ala Ala Gly Cys Asp Asp Thr Thr Phe Gln Pro Cys Ser 
    290                 295                 300                 
Ala Arg Ala Leu Ala Asn His Lys Val Val Thr Asp Ser Phe Arg Ser 
305                 310                 315                 320 
Ile Tyr Ser Ile Asn Ser Gly Ile Pro Gln Gly Gln Ala Val Ala Val 
                325                 330                 335     
Gly Arg Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Ala Trp Tyr Leu 
            340                 345                 350         
Cys Thr Leu Ala Ala Ala Glu Gln Leu Tyr Asp Ala Leu Tyr Gln Trp 
        355                 360                 365             
Asn Arg Ile Gly Ser Leu Thr Ile Thr Asp Val Ser Leu Ala Phe Phe 
    370                 375                 380                 
Gln Asp Leu Tyr Pro Ser Ala Ala Thr Gly Thr Tyr Ser Ser Ser Ser 
385                 390                 395                 400 
Ser Thr Tyr Gln Ser Ile Val Ala Ala Val Lys Thr Tyr Ala Asp Gly 
                405                 410                 415     
Tyr Met Ser Ile Val Gln Lys Tyr Thr Pro Ser Asn Gly Ala Leu Ala 
            420                 425                 430         
Glu Gln Phe Ser Arg Asn Asp Gly Ser Pro Leu Ser Ala Val Asp Leu 
        435                 440                 445             
Thr Trp Ser Tyr Ala Ser Leu Leu Thr Ala Ala Ala Arg Arg Asn Phe 
    450                 455                 460                 
Ser Val Pro Ala Tyr Ser Trp Gly Glu Ala Ser Ala Asn Thr Val Pro 
465                 470                 475                 480 
Ser Ser Cys Ser Ala Ser Ser Ala Ser Gly Pro Tyr Ala Thr Ala Thr 
                485                 490                 495     
Asn Thr Asn Trp Pro Ala Pro Thr Cys Thr Ser Pro Pro Ala Asn Val 
            500                 505                 510         
Ala Val Arg Phe Asn Glu Met Val Thr Thr Asn Phe Gly Glu Asn Val 
        515                 520                 525             
Phe Val Val Gly Ser Ile Ala Ala Leu Gly Ser Trp Ser Pro Ser Ser 
    530                 535                 540                 
Ala Ile Pro Leu Ser Ala Ala Glu Tyr Asn Ser Gln Thr Pro Leu Trp 
545                 550                 555                 560 
Tyr Ala Ile Val Thr Leu Pro Ala Gly Thr Ser Phe Gln Tyr Lys Tyr 
                565                 570                 575     
Ile Lys Lys Glu Pro Asp Gly Ser Val Val Trp Glu Ser Asp Pro Asn 
            580                 585                 590         
Arg Ser Tyr Thr Val Pro Gln Gly Cys Gly Val Thr Thr Ala Thr Val 
        595                 600                 605             
Asn Asp Ser Trp Arg 
    610             

<210>  2
<211>  593
<212>  PRT
<213>  嗜热蓝状菌JCM 12802(Talaromyces leycettanus JCM 12802)

<400>  2
Ala Pro His Pro Thr Glu Leu Leu Pro Arg Ala Ser Gly Ser Leu Asp 
1               5                   10                  15      
Ser Trp Leu Ser Thr Glu Val Pro Tyr Ala Leu Asp Gly Val Leu Asn 
            20                  25                  30          
Asn Ile Gly Pro Asn Gly Ala Lys Ala Gln Gly Ala Ser Ser Gly Ile 
        35                  40                  45              
Val Val Ala Ser Pro Ser Thr Ser Asn Pro Asp Tyr Phe Tyr Ser Trp 
    50                  55                  60                  
Thr Arg Asp Ala Ala Leu Thr Ile Lys Cys Leu Ile Asp Glu Phe Ile 
65                  70                  75                  80  
Ser Thr Gly Asp Ala Asn Leu Gln Ser Val Ile Gln Asn Tyr Ile Ser 
                85                  90                  95      
Ser Gln Ala Phe Leu Gln Thr Val Ser Asn Pro Ser Gly Gly Leu Ser 
            100                 105                 110         
Thr Gly Gly Leu Gly Glu Pro Lys Phe Glu Val Asn Glu Ala Ala Phe 
        115                 120                 125             
Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala 
    130                 135                 140                 
Thr Ala Met Ile Asn Tyr Ala Asn Trp Leu Ile Ala Asn Gly Gln Ala 
145                 150                 155                 160 
Ser Leu Ala Asn Ser Ile Val Trp Pro Ile Val Gln Asn Asp Leu Ser 
                165                 170                 175     
Tyr Val Ser Gln Tyr Trp Asn Gln Ser Thr Phe Asp Leu Trp Glu Glu 
            180                 185                 190         
Ile Asp Ser Ser Ser Phe Phe Thr Thr Ala Val Gln His Arg Ala Leu 
        195                 200                 205             
Val Glu Gly Ser Ala Leu Ala Lys Lys Leu Gly His Thr Cys Ser Asn 
    210                 215                 220                 
Cys Asp Ser Gln Ala Pro Leu Val Leu Cys Phe Leu Gln Ser Tyr Trp 
225                 230                 235                 240 
Thr Gly Ser Tyr Ile Leu Ser Asn Thr Gly Gly Gly Arg Ser Gly Lys 
                245                 250                 255     
Asp Ala Asn Ser Leu Leu Gly Ser Ile His Thr Phe Asp Pro Ala Ala 
            260                 265                 270         
Ala Gly Cys Asp Asp Thr Thr Phe Gln Pro Cys Ser Ala Arg Ala Leu 
        275                 280                 285             
Ala Asn His Lys Val Val Thr Asp Ser Phe Arg Ser Ile Tyr Ser Ile 
    290                 295                 300                 
Asn Ser Gly Ile Pro Gln Gly Gln Ala Val Ala Val Gly Arg Tyr Pro 
305                 310                 315                 320 
Glu Asp Val Tyr Gln Gly Gly Asn Ala Trp Tyr Leu Cys Thr Leu Ala 
                325                 330                 335     
Ala Ala Glu Gln Leu Tyr Asp Ala Leu Tyr Gln Trp Asn Arg Ile Gly 
            340                 345                 350         
Ser Leu Thr Ile Thr Asp Val Ser Leu Ala Phe Phe Gln Asp Leu Tyr 
        355                 360                 365             
Pro Ser Ala Ala Thr Gly Thr Tyr Ser Ser Ser Ser Ser Thr Tyr Gln 
    370                 375                 380                 
Ser Ile Val Ala Ala Val Lys Thr Tyr Ala Asp Gly Tyr Met Ser Ile 
385                 390                 395                 400 
Val Gln Lys Tyr Thr Pro Ser Asn Gly Ala Leu Ala Glu Gln Phe Ser 
                405                 410                 415     
Arg Asn Asp Gly Ser Pro Leu Ser Ala Val Asp Leu Thr Trp Ser Tyr 
            420                 425                 430         
Ala Ser Leu Leu Thr Ala Ala Ala Arg Arg Asn Phe Ser Val Pro Ala 
        435                 440                 445             
Tyr Ser Trp Gly Glu Ala Ser Ala Asn Thr Val Pro Ser Ser Cys Ser 
    450                 455                 460                 
Ala Ser Ser Ala Ser Gly Pro Tyr Ala Thr Ala Thr Asn Thr Asn Trp 
465                 470                 475                 480 
Pro Ala Pro Thr Cys Thr Ser Pro Pro Ala Asn Val Ala Val Arg Phe 
                485                 490                 495     
Asn Glu Met Val Thr Thr Asn Phe Gly Glu Asn Val Phe Val Val Gly 
            500                 505                 510         
Ser Ile Ala Ala Leu Gly Ser Trp Ser Pro Ser Ser Ala Ile Pro Leu 
        515                 520                 525             
Ser Ala Ala Glu Tyr Asn Ser Gln Thr Pro Leu Trp Tyr Ala Ile Val 
    530                 535                 540                 
Thr Leu Pro Ala Gly Thr Ser Phe Gln Tyr Lys Tyr Ile Lys Lys Glu 
545                 550                 555                 560 
Pro Asp Gly Ser Val Val Trp Glu Ser Asp Pro Asn Arg Ser Tyr Thr 
                565                 570                 575     
Val Pro Gln Gly Cys Gly Val Thr Thr Ala Thr Val Asn Asp Ser Trp 
            580                 585                 590         
Arg 
    

<210>  3
<211>  1990
<212>  DNA
<213>  嗜热蓝状菌JCM 12802(Talaromyces leycettanus JCM 12802)

<400>  3
ccctcgaggc atcagggtcc ctggattcat ggctttccac cgaagttcct tacgctctcg        60
atggtgtatt gaacaacatc ggacccaatg gtgcaaaggc ccagggggcc agctccggca       120
ttgtggttgc aagccccagc acaagtaatc ctgactgtaa gtcaacctgc attcattctg       180
ctatgaagaa gcctaactaa cgcatcctag acttctactc ttggactcgg gacgctgcgc       240
tcaccatcaa atgcctgatc gatgagttca tctcgactgg ggatgcgaac ctgcagtcgg       300
tgattcagaa ctatatcagc tcccaggcct tcttgcaaac agtgtccaac ccctctggcg       360
gcctgtcaac tggaggtctc ggcgagccca agtttgaggt caatgaggcg gcatttactg       420
gtgcttgggg ccggccacaa agagatgggc cggccttgag agcgactgcc atgatcaatt       480
acgccaactg gcttattgta agtggttctc acaggcgagt acatggctgc ggtatctgac       540
gaatgtcatg ccacaggcaa atggacaggc ttcactcgcc aattcgatcg tctggccgat       600
cgtccagaat gatctctcct acgtcagcca gtactggaat cagagtacct ttggtacggc       660
tagtccccca gagtggcctt tttctgtact gacgatgtct cagacctttg ggaggaaatc       720
gacagctcct ccttcttcac gacggctgtg cagcaccgtg ctcttgttga gggctctgct       780
ctggcaaaaa agcttggcca tacctgctca aactgcgact ctcaagcacc gcttgtcttg       840
tgtttcctgc aatcctactg gaccggttcc tatattcttt ccaacaccgg agcggacgtt       900
ccggaaagga cgccaactcc ctacttggaa gtattcatac ttttgaccag cagcggcggg       960
atgcgacgac accactttcc agccttgctc tgcccgagcc ctagcgaacc acaaggtcgt      1020
caccgactcg ttccgttcaa tctactcaat caactcgggc atcccacagg gccaagcagt      1080
cgccgtgggt cgctaccctg aagatgtata tcagggcgga aacgcatggt atctctgcac      1140
cctcgctgct gcagagcagc tgtacgacgc actctatcag tggaacagga tcggatctct      1200
cacgatcacg gacgtcagct tggcattctt ccaggatctc tacccatcgg cggcaacagg      1260
cacttattcc tcatcctcgt cgacctacca atccatcgtt gccgctgtca agacgtacgc      1320
ggacggatac atgagcattg ttgtaagtta ctgcatatcg ccaagttttt tccagcgctc      1380
tcaagagcac caagtgggaa aaaaaagtat aatactcact aaaccccttc tccaaacagc      1440
aaaaatacac cccttccaac ggcgccctcg ccgagcagtt ctcccgcaac gatggctccc      1500
ccctctcagc cgtcgaccta acctggtcct acgcctccct gctcactgcc gccgcgcgca      1560
gaaatttctc cgtccccgcc tactcctggg gcgaagccag cgccaacacc gtcccatcgt      1620
cttgctcggc ctcgtctgcc tcaggcccct atgccaccgc gaccaacacg aactggcccg      1680
cacccacatg cacctcgcca ccggcaaacg tggccgtccg attcaacgag atggtcacta      1740
ccaactttgg agagaacgtc tttgtcgtgg gctcgatcgc cgcgttggga tcttggagtc      1800
ctagttccgc tatcccgctg agcgcggccg aatacaactc acagacgccg ttgtggtatg      1860
caatcgtgac gttgccggcg ggcacgagct tccagtataa gtatatcaag aaagagccgg      1920
atggcagtgt ggtctgggag agtgatccga acaggtccta tacggtgcct caagggtgtg      1980
gcgtgaatta                                                             1990

<210>  4
<211>  1842
<212>  DNA
<213>  嗜热蓝状菌JCM 12802(Talaromyces leycettanus JCM 12802)

<400>  4
atgcagtacc ttcttaaaac taccctcggc gctctgagcg ttgctcagct tgtcatcgcg        60
gcaccacatc ccacggaact tctccctcgg gcatcagggt ccctggattc atggctttcc       120
accgaagttc cttacgctct cgatggtgta ttgaacaaca tcggacccaa tggtgcaaag       180
gcccaggggg ccagctccgg cattgtggtt gcaagcccca gcacaagtaa tcctgactac       240
ttctactctt ggactcggga cgctgcgctc accatcaaat gcctgatcga tgagttcatc       300
tcgactgggg atgcgaacct gcagtcggtg attcagaact atatcagctc ccaggccttc       360
ttgcaaacag tgtccaaccc ctctggcggc ctgtcaactg gaggtctcgg cgagcccaag       420
tttgaggtca atgaggcggc atttactggt gcttggggcc ggccacaaag agatgggccg       480
gccttgagag cgactgccat gatcaattac gccaactggc ttattgcaaa tggacaggct       540
tcactcgcca attcgatcgt ctggccgatc gtccagaatg atctctccta cgtcagccag       600
tactggaatc agagtacctt tgacctttgg gaggaaatcg acagctcctc cttcttcacg       660
acggctgtgc agcaccgtgc tcttgttgag ggctctgctc tggcaaaaaa gcttggccat       720
acctgctcaa actgcgactc tcaagcaccg cttgtcttgt gtttcctgca atcctactgg       780
accggttcct atattctttc caacaccgga ggcggacgtt ccggaaagga cgccaactcc       840
ctacttggaa gtattcatac ttttgaccca gcagcggcgg gatgcgacga caccactttc       900
cagccttgct ctgcccgagc cctagcgaac cacaaggtcg tcaccgactc gttccgttca       960
atctactcaa tcaactcggg catcccacag ggccaagcag tcgccgtggg tcgctaccct      1020
gaagatgtat atcagggcgg aaacgcatgg tatctctgca ccctcgctgc tgcagagcag      1080
ctgtacgacg cactctatca gtggaacagg atcggatctc tcacgatcac ggacgtcagc      1140
ttggcattct tccaggatct ctacccatcg gcggcaacag gcacttattc ctcatcctcg      1200
tcgacctacc aatccatcgt tgccgctgtc aagacgtacg cggacggata catgagcatt      1260
gttcaaaaat acaccccttc caacggcgcc ctcgccgagc agttctcccg caacgatggc      1320
tcccccctct cagccgtcga cctaacctgg tcctacgcct ccctgctcac tgccgccgcg      1380
cgcagaaatt tctccgtccc cgcctactcc tggggcgaag ccagcgccaa caccgtccca      1440
tcgtcttgct cggcctcgtc tgcctcaggc ccctatgcca ccgcgaccaa cacgaactgg      1500
cccgcaccca catgcacctc gccaccggca aacgtggccg tccgattcaa cgagatggtc      1560
actaccaact ttggagagaa cgtctttgtc gtgggctcga tcgccgcgtt gggatcttgg      1620
agtcctagtt ccgctatccc gctgagcgcg gccgaataca actcacagac gccgttgtgg      1680
tatgcaatcg tgacgttgcc ggcgggcacg agcttccagt ataagtatat caagaaagag      1740
ccggatggca gtgtggtctg ggagagtgat ccgaacaggt cctatacggt gcctcaaggg      1800
tgtggcgtga cgactgcgac ggtgaatgat agttggaggt ag                         1842

<210>  5
<211>  1782
<212>  DNA
<213>  嗜热蓝状菌JCM 12802(Talaromyces leycettanus JCM 12802)

<400>  5
gcaccacatc ccacggaact tctccctcgg gcatcagggt ccctggattc atggctttcc        60
accgaagttc cttacgctct cgatggtgta ttgaacaaca tcggacccaa tggtgcaaag       120
gcccaggggg ccagctccgg cattgtggtt gcaagcccca gcacaagtaa tcctgactac       180
ttctactctt ggactcggga cgctgcgctc accatcaaat gcctgatcga tgagttcatc       240
tcgactgggg atgcgaacct gcagtcggtg attcagaact atatcagctc ccaggccttc       300
ttgcaaacag tgtccaaccc ctctggcggc ctgtcaactg gaggtctcgg cgagcccaag       360
tttgaggtca atgaggcggc atttactggt gcttggggcc ggccacaaag agatgggccg       420
gccttgagag cgactgccat gatcaattac gccaactggc ttattgcaaa tggacaggct       480
tcactcgcca attcgatcgt ctggccgatc gtccagaatg atctctccta cgtcagccag       540
tactggaatc agagtacctt tgacctttgg gaggaaatcg acagctcctc cttcttcacg       600
acggctgtgc agcaccgtgc tcttgttgag ggctctgctc tggcaaaaaa gcttggccat       660
acctgctcaa actgcgactc tcaagcaccg cttgtcttgt gtttcctgca atcctactgg       720
accggttcct atattctttc caacaccgga ggcggacgtt ccggaaagga cgccaactcc       780
ctacttggaa gtattcatac ttttgaccca gcagcggcgg gatgcgacga caccactttc       840
cagccttgct ctgcccgagc cctagcgaac cacaaggtcg tcaccgactc gttccgttca       900
atctactcaa tcaactcggg catcccacag ggccaagcag tcgccgtggg tcgctaccct       960
gaagatgtat atcagggcgg aaacgcatgg tatctctgca ccctcgctgc tgcagagcag      1020
ctgtacgacg cactctatca gtggaacagg atcggatctc tcacgatcac ggacgtcagc      1080
ttggcattct tccaggatct ctacccatcg gcggcaacag gcacttattc ctcatcctcg      1140
tcgacctacc aatccatcgt tgccgctgtc aagacgtacg cggacggata catgagcatt      1200
gttcaaaaat acaccccttc caacggcgcc ctcgccgagc agttctcccg caacgatggc      1260
tcccccctct cagccgtcga cctaacctgg tcctacgcct ccctgctcac tgccgccgcg      1320
cgcagaaatt tctccgtccc cgcctactcc tggggcgaag ccagcgccaa caccgtccca      1380
tcgtcttgct cggcctcgtc tgcctcaggc ccctatgcca ccgcgaccaa cacgaactgg      1440
cccgcaccca catgcacctc gccaccggca aacgtggccg tccgattcaa cgagatggtc      1500
actaccaact ttggagagaa cgtctttgtc gtgggctcga tcgccgcgtt gggatcttgg      1560
agtcctagtt ccgctatccc gctgagcgcg gccgaataca actcacagac gccgttgtgg      1620
tatgcaatcg tgacgttgcc ggcgggcacg agcttccagt ataagtatat caagaaagag      1680
ccggatggca gtgtggtctg ggagagtgat ccgaacaggt cctatacggt gcctcaaggg      1740
tgtggcgtga cgactgcgac ggtgaatgat agttggaggt ag                         1782

<210>  6
<211>  20
<212>  PRT
<213>  嗜热蓝状菌JCM 12800(Talaromyces leycettanus JCM 12802)

<400>  6
Met Gln Tyr Leu Leu Lys Thr Thr Leu Gly Ala Leu Ser Val Ala Gln 
1               5                   10                  15      
Leu Val Ile Ala 
            20  

<210>  7
<211>  60
<212>  DNA
<213>  嗜热蓝状菌JCM 12800(Talaromyces leycettanus JCM 12802)

<400>  7
atgcagtacc ttcttaaaac taccctcggc gctctgagcg ttgctcagct tgtcatcgcg        60

<210>  8
<211>  34
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  8
ggggaattcg caccacatcc cacggaactt ctcc                                    34

<210>  9
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  9
tatgcggccg cctacctcca actatcattc accgtcgcag                              40

