                               SEQUENCE LISTING

<110> RESEARCH INSTITUTE AT NATIONWIDE CHILDREN'S HOSPITAL
 
<120> HMGB1 PROTEIN DERIVATIVES FOR THE REMOVAL OF BIOFILMS

<130> 106887-7910

<140> PCT/US2019/054851
<141> 2019-10-04

<150> 62/742,102
<151> 2018-10-05

<160> 16    

<170> PatentIn version 3.5

<210> 1
<211> 215
<212> PRT
<213> Mus sp.

<400> 1
Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Arg Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Glu Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val 
                165                 170                 175     


Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Gly Glu Glu 
            180                 185                 190         


Asp Glu Glu Asp Glu Glu Glu Glu Glu Asp Glu Glu Asp Glu Asp Glu 
        195                 200                 205             


Glu Glu Asp Asp Asp Asp Glu 
    210                 215 


<210> 2
<211> 215
<212> PRT
<213> Homo sapiens

<400> 2
Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val 
                165                 170                 175     


Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp Glu Glu 
            180                 185                 190         


Asp Glu Glu Asp Glu Glu Glu Glu Glu Asp Glu Glu Asp Glu Asp Glu 
        195                 200                 205             


Glu Glu Asp Asp Asp Asp Glu 
    210                 215 


<210> 3
<211> 87
<212> PRT
<213> Mus sp.

<400> 3
Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe 
1               5                   10                  15      


Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser 
            20                  25                  30          


Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala 
        35                  40                  45              


Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Glu Lys Leu Lys Glu 
    50                  55                  60                  


Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp 
65                  70                  75                  80  


Ala Ala Lys Lys Gly Val Val 
                85          


<210> 4
<211> 87
<212> PRT
<213> Homo sapiens

<400> 4
Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe 
1               5                   10                  15      


Cys Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser 
            20                  25                  30          


Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala 
        35                  40                  45              


Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu 
    50                  55                  60                  


Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp 
65                  70                  75                  80  


Ala Ala Lys Lys Gly Val Val 
                85          


<210> 5
<211> 87
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 5
Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe 
1               5                   10                  15      


Ser Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser 
            20                  25                  30          


Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala 
        35                  40                  45              


Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Glu Lys Leu Lys Glu 
    50                  55                  60                  


Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp 
65                  70                  75                  80  


Ala Ala Lys Lys Gly Val Val 
                85          


<210> 6
<211> 87
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 6
Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe 
1               5                   10                  15      


Ser Ser Glu Tyr Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser 
            20                  25                  30          


Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala 
        35                  40                  45              


Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu 
    50                  55                  60                  


Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp 
65                  70                  75                  80  


Ala Ala Lys Lys Gly Val Val 
                85          


<210> 7
<211> 176
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 7
Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Arg Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Glu Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val 
                165                 170                 175     


<210> 8
<211> 176
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 8
Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val 
                165                 170                 175     


<210> 9
<211> 97
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 9
Pro Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro 
1               5                   10                  15      


Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro 
            20                  25                  30          


Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys 
        35                  40                  45              


Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro 
    50                  55                  60                  


Tyr Glu Lys Lys Ala Glu Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile 
65                  70                  75                  80  


Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val 
                85                  90                  95      


Val 
    


<210> 10
<211> 97
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 10
Pro Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro 
1               5                   10                  15      


Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro 
            20                  25                  30          


Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys 
        35                  40                  45              


Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro 
    50                  55                  60                  


Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile 
65                  70                  75                  80  


Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val 
                85                  90                  95      


Val 
    


<210> 11
<211> 97
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 11
Pro Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro 
1               5                   10                  15      


Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Ser Ser Glu Tyr Arg Pro 
            20                  25                  30          


Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys 
        35                  40                  45              


Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro 
    50                  55                  60                  


Tyr Glu Lys Lys Ala Glu Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile 
65                  70                  75                  80  


Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val 
                85                  90                  95      


Val 
    


<210> 12
<211> 97
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Pro Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro 
1               5                   10                  15      


Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Ser Ser Glu Tyr Arg Pro 
            20                  25                  30          


Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys 
        35                  40                  45              


Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro 
    50                  55                  60                  


Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile 
65                  70                  75                  80  


Ala Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val 
                85                  90                  95      


Val 
    


<210> 13
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 13
Pro Pro Lys Gly Glu Thr Lys Lys Lys Phe 
1               5                   10  


<210> 14
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 14
Gly Pro Ser Leu Lys Leu 
1               5       


<210> 15
<211> 89
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 15
Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Arg Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe 
                85                  


<210> 16
<211> 89
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 16
Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe 
                85                  


