                         SEQUENCE LISTING

<110>  Arc Bio, LLC
       HARNESS, Eric
       NAGESH, Vaishnavi
       BENTLEY, Lloyd Gordon
       CARPENTER, Meredith
 
<120>  NORMALIZATION CONTROLS FOR MANAGING LOW SAMPLE INPUTS IN NEXT GENERATION SEQUENCING

<130>  ARCB-006/02WO

<150>  62/741,466
<151>  2018-10-04

<150>  62/832,560
<151>  2019-04-11

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA encoding sequence

<400>  1
gttttagagc tatgctgttt tg                                                22


<210>  2
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA encoding sequence

<400>  2
ggaaccattc aaaacagcat agcaagttaa aataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttttt                                            86


<210>  3
<211>  83
<212>  DNA
<213>  Artificial Squence

<220>
<223>  gNA stem-loop

<400>  3
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt ttt                                               83


<210>  4
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA stem-loop reverse complement

<400>  4
aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa       60

cttgctattt ctagctctaa aac                                               83


<210>  5
<211>  83
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gNA stem-loop

<400>  5
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu uuu                                               83


<210>  6
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA stem-loop

<400>  6
gttttagagc tatgctggaa acagcatagc aagttaaaat aaggctagtc cgttatcaac       60

ttgaaaaagt ggcaccgagt cggtgctttt tttc                                   94


<210>  7
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA stem-loop reverse complement

<400>  7
gaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta       60

acttgctatg ctgtttccag catagctcta aaac                                   94


<210>  8
<211>  94
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gNA stem-loop

<400>  8
guuuuagagc uaugcuggaa acagcauagc aaguuaaaau aaggcuaguc cguuaucaac       60

uugaaaaagu ggcaccgagu cggugcuuuu uuuc                                   94


<210>  9
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 protein binding sequence

<400>  9
aauuucuacu guuguagau                                                    19


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  encoding Cpf1 protein binding sequence

<400>  10
aatttctact gttgtagat                                                    19


<210>  11
<211>  86
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA sequence

<400>  11
ggaaccauuc aaaacagcau agcaaguuaa aauaaggcua guccguuauc aacuugaaaa       60

aguggcaccg agucggugcu uuuuuu                                            86


<210>  12
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  12
tgaagaactg cggcagg                                                      17


<210>  13
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  13
ggtctgcact tccagct                                                      17


<210>  14
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter

<400>  14
taatacgact cactatagg                                                    19


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter

<400>  15
taatacgact cactataggg                                                   20


<210>  16
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter

<400>  16
gcctcgagct aatacgactc actatagag                                         29


<210>  17
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6 promoter

<400>  17
atttaggtga cactatag                                                     18


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6 promoter

<400>  18
catacgattt aggtgacact atag                                              24


<210>  19
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3 promoter

<400>  19
aattaaccct cactaaag                                                     18


<210>  20
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  20
agctggaagt gcagacc                                                      17


<210>  21
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  i701-UM16 primer


<220>
<221>  misc_feature
<222>  (42)..(47)
<223>  n is a, c, g, or t

<400>  21
gatcggaaga gcacacgtct gaactccagt cactacaggt cnnnnnnatc tcgt             54


<210>  22
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  i702-UM16 primer


<220>
<221>  misc_feature
<222>  (42)..(47)
<223>  n is a, c, g, or t

<400>  22
gatcggaaga gcacacgtct gaactccagt cacagttaca tnnnnnnatc tcgt             54


<210>  23
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  i501-UM16 primer


<220>
<221>  misc_feature
<222>  (27)..(32)
<223>  n is a, c, g, or t

<400>  23
aatgatacgg cgaccgagat ctacacnnnn nnccagtaca acactctttc ccta             54


<210>  24
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  i502-UM6 primer


<220>
<221>  misc_feature
<222>  (30)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (58)..(58)
<223>  n is a, c, g, or t

<400>  24
aatgatacgg cgaccaccga gatgtaccan nnnnngtgct tatacactct ttggctan         58


