                         SEQUENCE LISTING

<110>  Bavarian Nordic A/S
 
<120>  COMBINATION THERAPY FOR TREATING CANCER WITH AN INTRAVENOUS 
       ADMINISTRATION OF A RECOMBINANT MVA AND AN IMMUNE CHECKPOINT 
       ANTAGONIST OR AGONIST

<130>  BNIT0013PCT

<150>  EP 18199002.9
<151>  2018-10-05

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  1145
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  synthetic
<222>  (1)..(1145)
<223>  Synthetic Her2 v1 amino acid sequence

<400>  1

Met Glu Leu Ala Ala Leu Cys Arg Trp Gly Leu Leu Leu Ala Leu Leu 
1               5                   10                  15      


Pro Pro Gly Ala Ala Ser Thr Gln Val Cys Thr Gly Thr Asp Met Lys 
            20                  25                  30          


Leu Arg Leu Pro Ala Ser Pro Glu Thr His Leu Asp Met Leu Arg His 
        35                  40                  45              


Leu Tyr Gln Gly Cys Gln Val Val Gln Gly Asn Leu Glu Leu Thr Tyr 
    50                  55                  60                  


Leu Pro Thr Asn Ala Ser Leu Ser Phe Leu Gln Asp Ile Gln Glu Val 
65                  70                  75                  80  


Gln Gly Tyr Val Leu Ile Ala His Asn Gln Val Arg Gln Val Pro Leu 
                85                  90                  95      


Gln Arg Leu Arg Ile Val Arg Gly Thr Gln Leu Phe Glu Asp Asn Tyr 
            100                 105                 110         


Ala Leu Ala Val Leu Asp Asn Gly Asp Pro Leu Asn Asn Thr Thr Pro 
        115                 120                 125             


Val Thr Gly Ala Ser Pro Gly Gly Leu Arg Glu Leu Gln Leu Arg Ser 
    130                 135                 140                 


Leu Thr Glu Ile Leu Lys Gly Gly Val Leu Ile Gln Arg Asn Pro Gln 
145                 150                 155                 160 


Leu Cys Tyr Gln Asp Thr Ile Leu Trp Lys Asp Ile Phe His Lys Asn 
                165                 170                 175     


Asn Gln Leu Ala Leu Thr Leu Ile Asp Thr Asn Arg Ser Arg Ala Cys 
            180                 185                 190         


His Pro Cys Ser Pro Met Cys Lys Gly Ser Arg Cys Trp Gly Glu Ser 
        195                 200                 205             


Ser Glu Asp Cys Gln Ser Leu Thr Arg Thr Val Cys Ala Gly Gly Cys 
    210                 215                 220                 


Ala Arg Cys Lys Gly Pro Leu Pro Thr Asp Cys Cys His Glu Gln Cys 
225                 230                 235                 240 


Ala Ala Gly Cys Thr Gly Pro Lys His Ser Asp Cys Leu Ala Cys Leu 
                245                 250                 255     


His Phe Asn His Ser Gly Ile Cys Glu Leu His Cys Pro Ala Leu Val 
            260                 265                 270         


Thr Tyr Asn Thr Arg Thr Phe Lys Ser Met Pro Asn Pro Glu Gly Arg 
        275                 280                 285             


Tyr Thr Phe Gly Ala Ser Cys Val Thr Ala Cys Pro Tyr Asn Tyr Leu 
    290                 295                 300                 


Ser Thr Asp Val Gly Ser Cys Thr Leu Val Cys Pro Ala Ala Asn Gln 
305                 310                 315                 320 


Glu Val Thr Ala Glu Asp Gly Thr Gln Arg Cys Glu Lys Cys Ser Lys 
                325                 330                 335     


Pro Cys Ala Arg Val Cys Tyr Gly Leu Gly Met Glu His Leu Arg Glu 
            340                 345                 350         


Val Arg Ala Val Thr Ser Ala Asn Ile Gln Glu Phe Ala Gly Cys Lys 
        355                 360                 365             


Lys Ile Phe Gly Ser Leu Ala Phe Leu Pro Glu Ser Phe Asp Gly Asp 
    370                 375                 380                 


Pro Ala Ser Asn Thr Ala Pro Leu Gln Pro Glu Gln Leu Gln Val Phe 
385                 390                 395                 400 


Glu Thr Leu Glu Glu Ile Thr Gly Tyr Leu Tyr Ile Ser Ala Trp Pro 
                405                 410                 415     


Asp Ser Leu Pro Asp Leu Ser Val Phe Gln Asn Leu Gln Val Ile Arg 
            420                 425                 430         


Gly Arg Ile Leu His Asn Gly Ala Tyr Ser Leu Thr Leu Gln Gly Leu 
        435                 440                 445             


Gly Ile Ser Trp Leu Gly Leu Arg Ser Leu Arg Glu Leu Gly Ser Gly 
    450                 455                 460                 


Leu Ala Leu Ile His His Asn Thr His Leu Cys Phe Val His Thr Val 
465                 470                 475                 480 


Pro Trp Asp Gln Leu Phe Arg Asn Pro His Gln Ala Leu Leu His Thr 
                485                 490                 495     


Ala Asn Arg Pro Glu Asp Glu Cys Val Gly Glu Gly Leu Ala Cys His 
            500                 505                 510         


Gln Leu Cys Ala Arg Gly His Cys Trp Gly Pro Gly Pro Thr Gln Cys 
        515                 520                 525             


Val Asn Cys Ser Gln Phe Leu Arg Gly Gln Glu Cys Val Glu Glu Cys 
    530                 535                 540                 


Arg Val Leu Gln Gly Leu Pro Arg Glu Tyr Val Asn Ala Arg His Cys 
545                 550                 555                 560 


Leu Pro Cys His Pro Glu Cys Gln Pro Gln Asn Gly Ser Val Thr Cys 
                565                 570                 575     


Phe Gly Pro Ala Ala Asp Gln Cys Val Ala Cys Ala His Tyr Lys Asp 
            580                 585                 590         


Pro Pro Ala Cys Val Ala Arg Cys Pro Ser Gly Val Lys Pro Asp Leu 
        595                 600                 605             


Ser Tyr Met Pro Ile Trp Ala Phe Pro Asp Glu Glu Gly Ala Cys Gln 
    610                 615                 620                 


Pro Cys Pro Ile Asn Cys Thr His Ser Cys Val Asp Leu Asp Asp Lys 
625                 630                 635                 640 


Gly Cys Pro Ala Glu Gln Arg Ala Ser Pro Leu Thr Ser Ile Ile Ser 
                645                 650                 655     


Ala Val Val Gly Ile Leu Leu Val Val Val Leu Gly Val Val Phe Gly 
            660                 665                 670         


Ile Leu Ile Lys Arg Arg Gln Gln Lys Ile Arg Lys Tyr Thr Met Arg 
        675                 680                 685             


Arg Leu Leu Gln Glu Thr Glu Leu Val Glu Pro Leu Thr Pro Ser Gly 
    690                 695                 700                 


Ala Met Pro Asn Gln Ala Gln Met Arg Ile Leu Lys Glu Thr Glu Leu 
705                 710                 715                 720 


Arg Lys Val Lys Val Leu Gly Ser Gly Ala Phe Gly Thr Val Tyr Lys 
                725                 730                 735     


Gly Ile Trp Ile Pro Asp Gly Glu Asn Val Lys Ile Pro Val Ala Ile 
            740                 745                 750         


Met Val Leu Arg Glu Asn Thr Ser Pro Lys Ala Asn Lys Glu Ile Leu 
        755                 760                 765             


Asp Glu Ala Tyr Val Met Ala Gly Val Gly Ser Pro Tyr Val Ser Arg 
    770                 775                 780                 


Leu Leu Gly Ile Cys Leu Thr Ser Thr Val Gln Leu Val Thr Gln Leu 
785                 790                 795                 800 


Met Pro Tyr Gly Cys Leu Leu Asp His Val Arg Glu Asn Arg Gly Arg 
                805                 810                 815     


Leu Gly Ser Gln Asp Leu Leu Asn Trp Cys Met Gln Ile Ala Lys Gly 
            820                 825                 830         


Met Ser Tyr Leu Glu Asp Val Arg Leu Val His Arg Asp Leu Ala Ala 
        835                 840                 845             


Arg Asn Val Leu Val Lys Ser Pro Asn His Val Lys Ile Thr Asp Phe 
    850                 855                 860                 


Gly Leu Ala Arg Leu Leu Asp Ile Asp Glu Thr Glu Tyr His Ala Asp 
865                 870                 875                 880 


Gly Gly Lys Val Pro Ile Lys Trp Met Ala Leu Glu Ser Ile Leu Arg 
                885                 890                 895     


Arg Arg Phe Thr His Gln Ser Asp Val Trp Ser Tyr Gly Val Thr Val 
            900                 905                 910         


Trp Glu Leu Met Thr Phe Gly Ala Lys Pro Tyr Asp Gly Ile Pro Ala 
        915                 920                 925             


Arg Glu Ile Pro Asp Leu Leu Glu Lys Gly Glu Arg Leu Pro Gln Pro 
    930                 935                 940                 


Pro Ile Cys Thr Ile Asp Val Tyr Met Ile Met Val Lys Cys Trp Met 
945                 950                 955                 960 


Ile Asp Ser Glu Cys Arg Pro Arg Phe Arg Glu Leu Val Ser Glu Phe 
                965                 970                 975     


Ser Arg Met Ala Arg Asp Pro Gln Arg Phe Val Val Ile Gln Asn Glu 
            980                 985                 990         


Asp Leu Gly Pro Ala Ser Pro Leu  Asp Ser Thr Phe Tyr  Arg Ser Leu 
        995                 1000                 1005             


Leu Glu  Asp Asp Asp Met Gly  Asp Leu Val Asp Ala  Glu Glu Ala 
    1010                 1015                 1020             


Leu Val  Pro Gln Gln Gly Phe  Phe Cys Pro Asp Pro  Ala Pro Gly 
    1025                 1030                 1035             


Ala Gly  Gly Met Val His His  Arg His Arg Ser Ser  Ser Thr Arg 
    1040                 1045                 1050             


Ser Gly  Gly Gly Asp Leu Thr  Leu Gly Leu Glu Pro  Ser Glu Glu 
    1055                 1060                 1065             


Glu Ala  Pro Arg Ser Pro Leu  Ala Pro Ser Glu Gly  Ala Gly Ser 
    1070                 1075                 1080             


Asp Val  Phe Asp Gly Asp Leu  Gly Met Gly Ala Ala  Lys Gly Leu 
    1085                 1090                 1095             


Gln Ser  Leu Pro Thr His Asp  Pro Ser Pro Leu Gln  Arg Tyr Ser 
    1100                 1105                 1110             


Glu Asp  Pro Thr Val Pro Leu  Pro Ser Glu Thr Asp  Gly Tyr Val 
    1115                 1120                 1125             


Ala Pro  Leu Thr Cys Ser Pro  Gln Pro Glu Leu Gly  Leu Asp Val 
    1130                 1135                 1140             


Pro Val  
    1145 


<210>  2
<211>  3441
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  synthetic
<222>  (1)..(3441)
<223>  Synthetic Her2 v1 nucleotide sequence

<400>  2
atggaactgg ctgctctgtg tagatgggga ctgctgcttg ctctgttgcc tcctggagct       60

gcttctaccc aagtgtgcac aggcaccgac atgaagctga gactgcctgc ttctcctgag      120

acacacctgg acatgctgag acacctgtac cagggatgtc aggtggtgca gggaaatctg      180

gaactgacct acctgcctac caacgccagc ctgagctttc tgcaggacat ccaagaggtg      240

cagggatacg tgctgatcgc tcacaatcaa gtgagacagg tgccactgca gaggctgaga      300

atcgttagag gcacccagct gttcgaggac aactatgctc tggctgtgct ggacaatggc      360

gaccctctga acaacaccac acctgtgaca ggagcttctc ctggtggact gagagaactg      420

cagctgagaa gcctgaccga gatcctgaaa ggaggagtgc tgatccagcg gaaccctcag      480

ctgtgctacc aggacaccat cctgtggaag gacatcttcc acaagaacaa ccagctggct      540

ctgacactga tcgacaccaa cagaagcaga gcctgccatc cttgctctcc catgtgcaag      600

ggctctagat gttggggaga gagcagcgag gattgccaga gcctgaccag aacagtgtgt      660

gctggaggat gtgccagatg caaaggacct ctgcctaccg actgctgcca cgagcaatgt      720

gcagctggat gtacaggacc aaagcactct gattgcctgg cctgcctgca cttcaaccac      780

tctggaatct gcgagctgca ctgtcctgct ctggtcacct acaacacacg gaccttcaag      840

agcatgccta atcctgaagg cagatacacc tttggagcca gctgtgtgac agcctgtcct      900

tacaactacc tgagcaccga cgtgggcagc tgcacactcg tttgtcctgc tgccaatcaa      960

gaagtgacag ccgaggacgg cacccagaga tgcgagaagt gtagcaagcc ttgcgctaga     1020

gtgtgttacg gactcggcat ggaacacctg agagaagtga gagccgtgac cagtgccaac     1080

atccaagagt ttgctggctg caagaagatc tttggcagcc tcgccttcct gcctgagagc     1140

ttcgatggcg atcctgccag caatactgct cctctgcagc ctgaacagct ccaggtgttc     1200

gagacactgg aagagatcac aggctacctg tacatcagcg catggccaga cagcctgcct     1260

gacctgtccg tgttccagaa cctgcaagtg atcagaggca gaatcctgca caacggagcc     1320

tattctctga ccctgcaagg cctgggaatc agctggctgg gactgagatc cctgagagag     1380

cttggatctg gcctggctct gatccaccac aatacccacc tgtgcttcgt gcacaccgtg     1440

ccttgggacc agctgtttcg gaatcctcat caggctctgc tgcacacagc caacagacct     1500

gaggatgagt gtgttggcga aggcctggct tgtcaccagc tctgtgctag aggacactgt     1560

tggggacctg gacctacaca gtgtgtgaac tgtagccagt tcctgagagg ccaagaatgc     1620

gtggaagagt gtagagttct gcagggactg cctcgcgagt acgtgaacgc tagacactgt     1680

ctgccttgtc atcccgagtg ccagcctcag aatggcagcg tgacatgttt tggaccagct     1740

gccgatcagt gcgtggcctg tgctcactat aaggaccctc cagcctgcgt ggccagatgt     1800

cctagcggag tgaagcctga cctgagctac atgcccatct gggcatttcc agatgaggaa     1860

ggagcttgcc agccttgtcc tatcaactgc acccacagct gcgtggacct ggacgataag     1920

ggatgtccag ccgagcagag agcctctcca ctgacctcta tcatctctgc cgtcgtgggc     1980

atcctgctgg tggtggttct gggagttgtg ttcggcatcc tgatcaagag acggcagcag     2040

aagatccgga agtacaccat gcggagactg ctgcaagaga ctgagctggt ggaacctctg     2100

acacccagcg gagctatgcc taaccaggct cagatgcgga ttctgaaaga aaccgagctg     2160

cggaaagtga aggtgctcgg ctctggagcc tttggcacag tgtacaaagg catctggatc     2220

cctgacggag agaacgtgaa gattcctgtg gccatcatgg tgctgagaga gaacacaagt     2280

cccaaggcca acaaagagat cctggacgag gcctacgtga tggctggtgt tggcagccct     2340

tatgtgtcta gactgctggg catctgtctg accagcaccg tgcagctggt cactcagctg     2400

atgccttacg gctgcctgct ggatcacgtg agagagaata gaggcagact gggctctcag     2460

gacctgctga actggtgcat gcagatcgcc aagggcatga gctacctcga ggatgtgaga     2520

ctggtccaca gagatctggc tgccagaaac gtgctcgtga agtctcctaa ccacgtgaag     2580

atcaccgact tcggactggc taggctgctg gatatcgacg agacagagta ccacgctgat     2640

ggaggcaagg tgcccatcaa gtggatggct ctggaatcca tcctgagacg gagattcacc     2700

caccagtccg atgtgtggtc ttacggagtg acagtgtggg agctgatgac cttcggagcc     2760

aagccttacg acggcatccc tgccagagag atcccagatc tgctggaaaa gggagagaga     2820

ctgcctcagc ctcctatctg caccatcgac gtgtacatga ttatggtcaa gtgttggatg     2880

atcgacagcg agtgcagacc cagattcaga gaactggtgt ccgagttctc tcggatggcc     2940

agagatcctc agagattcgt ggtcatccag aacgaggatc tgggacctgc cagccctctg     3000

gacagcacct tctacagatc cctgctggaa gatgacgaca tgggtgacct ggtggacgct     3060

gaagaagctc tggttcctca gcagggcttc ttctgccctg atcctgctcc aggagcaggt     3120

ggaatggtgc atcacagaca cagaagctcc agcaccagaa gcggaggcgg agatctgaca     3180

ctgggactcg agccatctga ggaagaggct cctagatctc ctctggctcc ttctgaagga     3240

gctggaagcg acgttttcga cggagatctt ggaatgggag ctgccaaagg actccagtct     3300

ctgcccacac acgacccatc tccactgcag agatacagcg aggaccctac cgtgcctctg     3360

ccaagcgaga cagatggata tgtggcacct ctgacctgct ctcctcagcc agaactggga     3420

cttgatgtgc ctgtttgatg a                                               3441


<210>  3
<211>  427
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  synthetic
<222>  (1)..(427)
<223>  Synthetic Brachyury amino acid sequence

<400>  3

Met Ser Ser Pro Gly Thr Glu Ser Ala Gly Lys Ser Leu Gln Tyr Arg 
1               5                   10                  15      


Val Asp His Leu Leu Ser Ala Val Glu Asn Glu Leu Gln Ala Gly Ser 
            20                  25                  30          


Glu Lys Gly Asp Pro Thr Glu Arg Glu Leu Arg Val Gly Leu Glu Glu 
        35                  40                  45              


Ser Glu Leu Trp Leu Arg Phe Lys Glu Leu Thr Asn Glu Met Ile Val 
    50                  55                  60                  


Thr Lys Asn Gly Arg Arg Met Phe Pro Val Leu Lys Val Asn Val Ser 
65                  70                  75                  80  


Gly Leu Asp Pro Asn Ala Met Tyr Ser Phe Leu Leu Asp Phe Val Ala 
                85                  90                  95      


Ala Asp Asn His Arg Trp Lys Tyr Val Asn Gly Glu Trp Val Pro Gly 
            100                 105                 110         


Gly Lys Pro Glu Pro Gln Ala Pro Ser Cys Val Tyr Ile His Pro Asp 
        115                 120                 125             


Ser Pro Asn Phe Gly Ala His Trp Met Lys Ala Pro Val Ser Phe Ser 
    130                 135                 140                 


Lys Val Lys Leu Thr Asn Lys Leu Asn Gly Gly Gly Gln Ile Met Leu 
145                 150                 155                 160 


Asn Ser Leu His Lys Tyr Glu Pro Arg Ile His Ile Val Arg Val Gly 
                165                 170                 175     


Gly Pro Gln Arg Met Ile Thr Ser His Cys Phe Pro Glu Thr Gln Phe 
            180                 185                 190         


Ile Ala Val Thr Ala Tyr Gln Asn Glu Glu Ile Thr Ala Leu Lys Ile 
        195                 200                 205             


Lys Tyr Asn Pro Phe Ala Lys Ala Phe Leu Asp Ala Lys Glu Arg Ser 
    210                 215                 220                 


Asp His Lys Glu Met Met Glu Glu Pro Gly Asp Ser Gln Gln Pro Gly 
225                 230                 235                 240 


Tyr Ser Gln Trp Gly Trp Leu Leu Pro Gly Thr Ser Thr Leu Cys Pro 
                245                 250                 255     


Pro Ala Asn Pro His Pro Gln Phe Gly Gly Ala Leu Ser Leu Pro Ser 
            260                 265                 270         


Thr His Ser Cys Asp Arg Tyr Pro Thr Leu Arg Ser His Tyr Ala His 
        275                 280                 285             


Arg Asn Asn Ser Pro Thr Tyr Ser Asp Asn Ser Pro Ala Cys Leu Ser 
    290                 295                 300                 


Met Leu Gln Ser His Asp Asn Trp Ser Ser Leu Gly Met Pro Ala His 
305                 310                 315                 320 


Pro Ser Met Leu Pro Val Ser His Asn Ala Ser Pro Pro Thr Ser Ser 
                325                 330                 335     


Ser Gln Tyr Pro Ser Leu Trp Ser Val Ser Asn Gly Ala Val Thr Pro 
            340                 345                 350         


Gly Ser Gln Ala Ala Ala Val Ser Asn Gly Leu Gly Ala Gln Phe Phe 
        355                 360                 365             


Arg Gly Ser Pro Ala His Tyr Thr Pro Leu Thr His Pro Val Ser Ala 
    370                 375                 380                 


Pro Ser Ser Ser Gly Ser Pro Leu Tyr Glu Gly Ala Ala Ala Ala Thr 
385                 390                 395                 400 


Asp Ile Val Asp Ser Gln Tyr Asp Ala Ala Ala Gln Gly Arg Leu Ile 
                405                 410                 415     


Ala Ser Trp Thr Pro Val Ser Pro Pro Ser Met 
            420                 425         


<210>  4
<211>  1287
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  synthetic
<222>  (1)..(1287)
<223>  Synthetic Brachyury nucleotide sequence

<400>  4
atgtctagcc ctggcacaga gtctgctggc aagagcctcc agtacagagt ggaccatctg       60

ctgagcgctg tggagaatga actgcaggct ggaagcgaga agggagatcc tacagaaaga      120

gagctgagag tcggactgga agagtccgag ctgtggctgc ggttcaaaga actgaccaac      180

gagatgatcg tgaccaagaa cggcagacgg atgttccctg tgctgaaagt gaacgtgtcc      240

ggactggacc ctaacgccat gtacagcttt ctgctggatt tcgtggcagc tgacaaccac      300

agatggaagt acgtgaacgg agagtgggtg ccaggaggaa aacctgaacc tcaggctcct      360

agctgcgtgt acattcaccc tgacagccct aacttcggag cccactggat gaaggctcct      420

gtgtccttca gcaaagtgaa gctgaccaac aagctgaacg gaggaggcca gatcatgctg      480

aacagcctgc acaagtatga gcctaggatc cacatcgtca gagttggagg ccctcagcgg      540

atgatcacca gccactgttt ccctgagaca cagttcatcg cagtgaccgc ttaccagaac      600

gaggaaatca cagccctgaa gatcaagtac aatcccttcg ccaaggcctt cctggacgcc      660

aaagagcgga gcgaccacaa agaaatgatg gaagaacctg gcgacagcca gcagcctggc      720

tattctcaat ggggatggct gctgccaggc acctccacat tgtgccctcc agccaatcct      780

catcctcagt ttggcggagc cctgagcctg cctagcacac acagctgcga cagataccct      840

acactgagaa gccactacgc tcacagaaac aacagcccta cctacagcga caatagccct      900

gcctgtctga gcatgctgca gtcccacgac aattggtcca gcctgggaat gcctgctcac      960

ccttctatgc tgcctgtctc tcacaacgcc tctccaccta caagcagctc tcagtaccct     1020

agcctttgga gcgtgtccaa tggagctgtg acacctggat ctcaggctgc cgctgtgtct     1080

aatggactgg gagcccagtt cttcagaggc agccctgctc actacacacc tctgacacat     1140

ccagtgtctg ctcctagcag cagcggaagc cctctctatg aaggagccgc tgcagccacc     1200

gacatcgtgg attctcagta tgatgctgcc gcacagggca gactgatcgc ctcttggaca     1260

cctgtgagcc caccttccat gtgatga                                         1287


<210>  5
<211>  435
<212>  PRT
<213>  Homo sapiens


<220>
<221>  Isoform
<222>  (1)..(435)
<223>  Brachyury protein Isoform 1 from GenBank Accession No. O15178.1

<400>  5

Met Ser Ser Pro Gly Thr Glu Ser Ala Gly Lys Ser Leu Gln Tyr Arg 
1               5                   10                  15      


Val Asp His Leu Leu Ser Ala Val Glu Asn Glu Leu Gln Ala Gly Ser 
            20                  25                  30          


Glu Lys Gly Asp Pro Thr Glu Arg Glu Leu Arg Val Gly Leu Glu Glu 
        35                  40                  45              


Ser Glu Leu Trp Leu Arg Phe Lys Glu Leu Thr Asn Glu Met Ile Val 
    50                  55                  60                  


Thr Lys Asn Gly Arg Arg Met Phe Pro Val Leu Lys Val Asn Val Ser 
65                  70                  75                  80  


Gly Leu Asp Pro Asn Ala Met Tyr Ser Phe Leu Leu Asp Phe Val Ala 
                85                  90                  95      


Ala Asp Asn His Arg Trp Lys Tyr Val Asn Gly Glu Trp Val Pro Gly 
            100                 105                 110         


Gly Lys Pro Glu Pro Gln Ala Pro Ser Cys Val Tyr Ile His Pro Asp 
        115                 120                 125             


Ser Pro Asn Phe Gly Ala His Trp Met Lys Ala Pro Val Ser Phe Ser 
    130                 135                 140                 


Lys Val Lys Leu Thr Asn Lys Leu Asn Gly Gly Gly Gln Ile Met Leu 
145                 150                 155                 160 


Asn Ser Leu His Lys Tyr Glu Pro Arg Ile His Ile Val Arg Val Gly 
                165                 170                 175     


Gly Pro Gln Arg Met Ile Thr Ser His Cys Phe Pro Glu Thr Gln Phe 
            180                 185                 190         


Ile Ala Val Thr Ala Tyr Gln Asn Glu Glu Ile Thr Ala Leu Lys Ile 
        195                 200                 205             


Lys Tyr Asn Pro Phe Ala Lys Ala Phe Leu Asp Ala Lys Glu Arg Ser 
    210                 215                 220                 


Asp His Lys Glu Met Met Glu Glu Pro Gly Asp Ser Gln Gln Pro Gly 
225                 230                 235                 240 


Tyr Ser Gln Trp Gly Trp Leu Leu Pro Gly Thr Ser Thr Leu Cys Pro 
                245                 250                 255     


Pro Ala Asn Pro His Pro Gln Phe Gly Gly Ala Leu Ser Leu Pro Ser 
            260                 265                 270         


Thr His Ser Cys Asp Arg Tyr Pro Thr Leu Arg Ser His Arg Ser Ser 
        275                 280                 285             


Pro Tyr Pro Ser Pro Tyr Ala His Arg Asn Asn Ser Pro Thr Tyr Ser 
    290                 295                 300                 


Asp Asn Ser Pro Ala Cys Leu Ser Met Leu Gln Ser His Asp Asn Trp 
305                 310                 315                 320 


Ser Ser Leu Gly Met Pro Ala His Pro Ser Met Leu Pro Val Ser His 
                325                 330                 335     


Asn Ala Ser Pro Pro Thr Ser Ser Ser Gln Tyr Pro Ser Leu Trp Ser 
            340                 345                 350         


Val Ser Asn Gly Ala Val Thr Pro Gly Ser Gln Ala Ala Ala Val Ser 
        355                 360                 365             


Asn Gly Leu Gly Ala Gln Phe Phe Arg Gly Ser Pro Ala His Tyr Thr 
    370                 375                 380                 


Pro Leu Thr His Pro Val Ser Ala Pro Ser Ser Ser Gly Ser Pro Leu 
385                 390                 395                 400 


Tyr Glu Gly Ala Ala Ala Ala Thr Asp Ile Val Asp Ser Gln Tyr Asp 
                405                 410                 415     


Ala Ala Ala Gln Gly Arg Leu Ile Ala Ser Trp Thr Pro Val Ser Pro 
            420                 425                 430         


Pro Ser Met 
        435 


<210>  6
<211>  1308
<212>  DNA
<213>  Homo sapiens


<220>
<221>  Isoform
<222>  (1)..(1308)
<223>  Coding sequence for Brachyury protein Isoform 1 GenBank Accession
       No. O15178.1

<400>  6
atgagcagcc ctggcacaga gagcgccggc aagagcctgc agtaccgggt ggaccatctg       60

ctgagcgccg tggagaatga gctgcaggcc ggctccgaga agggcgaccc caccgagagg      120

gaactgagag tgggcctgga agagtccgag ctgtggctgc ggttcaaaga actgaccaac      180

gagatgatcg tgaccaagaa cggcagacgg atgttccccg tgctgaaagt gaacgtgtcc      240

ggcctggacc ccaacgccat gtacagcttt ctgctggact tcgtggccgc cgacaaccac      300

aggtggaaat acgtgaacgg cgagtgggtg ccaggcggca aacctgagcc tcaggccccc      360

agctgcgtgt acatccaccc cgacagcccc aatttcggcg cccactggat gaaggccccc      420

gtgtccttca gcaaagtgaa gctgaccaac aagctgaacg gcggaggcca gatcatgctg      480

aacagcctgc acaagtacga gccccggatc cacattgtgc gcgtgggcgg accccagaga      540

atgatcacca gccactgctt ccccgagaca cagtttatcg ccgtgaccgc ctaccagaac      600

gaggaaatca ccgccctgaa gatcaagtac aaccccttcg ccaaggcctt cctggacgcc      660

aaagagcgga gcgaccacaa agaaatgatg gaagaacccg gcgacagcca gcagcctggc      720

tacagccagt ggggctggct gctgccaggc acctccactc tgtgcccccc tgccaaccct      780

caccctcagt tcggcggagc cctgagcctg cctagcacac acagctgcga cagatacccc      840

accctgcgga gccacagaag cagcccctac cccagcccat acgcccaccg gaacaacagc      900

cccacctaca gcgacaactc ccccgcctgc ctgagcatgc tgcagagcca cgacaactgg      960

tccagcctgg gcatgcctgc ccaccctagc atgctgcccg tgtcccacaa tgccagcccc     1020

cctaccagca gctcccagta ccctagcctg tggagcgtgt ccaatggcgc cgtgacacct     1080

ggatctcagg ccgctgccgt gagcaatggc ctgggagccc agttctttag aggcagccct     1140

gcccactaca cccctctgac ccaccctgtg tccgccccta gctccagcgg cagccctctg     1200

tatgaaggcg ccgctgcagc caccgatatc gtggacagcc agtacgatgc cgccgctcag     1260

ggcagactga tcgccagctg gacccccgtg tctcccccca gcatgtga                  1308


<210>  7
<211>  435
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  Isoform
<222>  (1)..(435)
<223>  Brachyury protein Isoform 1 (L254V)

<400>  7

Met Ser Ser Pro Gly Thr Glu Ser Ala Gly Lys Ser Leu Gln Tyr Arg 
1               5                   10                  15      


Val Asp His Leu Leu Ser Ala Val Glu Asn Glu Leu Gln Ala Gly Ser 
            20                  25                  30          


Glu Lys Gly Asp Pro Thr Glu Arg Glu Leu Arg Val Gly Leu Glu Glu 
        35                  40                  45              


Ser Glu Leu Trp Leu Arg Phe Lys Glu Leu Thr Asn Glu Met Ile Val 
    50                  55                  60                  


Thr Lys Asn Gly Arg Arg Met Phe Pro Val Leu Lys Val Asn Val Ser 
65                  70                  75                  80  


Gly Leu Asp Pro Asn Ala Met Tyr Ser Phe Leu Leu Asp Phe Val Ala 
                85                  90                  95      


Ala Asp Asn His Arg Trp Lys Tyr Val Asn Gly Glu Trp Val Pro Gly 
            100                 105                 110         


Gly Lys Pro Glu Pro Gln Ala Pro Ser Cys Val Tyr Ile His Pro Asp 
        115                 120                 125             


Ser Pro Asn Phe Gly Ala His Trp Met Lys Ala Pro Val Ser Phe Ser 
    130                 135                 140                 


Lys Val Lys Leu Thr Asn Lys Leu Asn Gly Gly Gly Gln Ile Met Leu 
145                 150                 155                 160 


Asn Ser Leu His Lys Tyr Glu Pro Arg Ile His Ile Val Arg Val Gly 
                165                 170                 175     


Gly Pro Gln Arg Met Ile Thr Ser His Cys Phe Pro Glu Thr Gln Phe 
            180                 185                 190         


Ile Ala Val Thr Ala Tyr Gln Asn Glu Glu Ile Thr Ala Leu Lys Ile 
        195                 200                 205             


Lys Tyr Asn Pro Phe Ala Lys Ala Phe Leu Asp Ala Lys Glu Arg Ser 
    210                 215                 220                 


Asp His Lys Glu Met Met Glu Glu Pro Gly Asp Ser Gln Gln Pro Gly 
225                 230                 235                 240 


Tyr Ser Gln Trp Gly Trp Leu Leu Pro Gly Thr Ser Thr Val Cys Pro 
                245                 250                 255     


Pro Ala Asn Pro His Pro Gln Phe Gly Gly Ala Leu Ser Leu Pro Ser 
            260                 265                 270         


Thr His Ser Cys Asp Arg Tyr Pro Thr Leu Arg Ser His Arg Ser Ser 
        275                 280                 285             


Pro Tyr Pro Ser Pro Tyr Ala His Arg Asn Asn Ser Pro Thr Tyr Ser 
    290                 295                 300                 


Asp Asn Ser Pro Ala Cys Leu Ser Met Leu Gln Ser His Asp Asn Trp 
305                 310                 315                 320 


Ser Ser Leu Gly Met Pro Ala His Pro Ser Met Leu Pro Val Ser His 
                325                 330                 335     


Asn Ala Ser Pro Pro Thr Ser Ser Ser Gln Tyr Pro Ser Leu Trp Ser 
            340                 345                 350         


Val Ser Asn Gly Ala Val Thr Pro Gly Ser Gln Ala Ala Ala Val Ser 
        355                 360                 365             


Asn Gly Leu Gly Ala Gln Phe Phe Arg Gly Ser Pro Ala His Tyr Thr 
    370                 375                 380                 


Pro Leu Thr His Pro Val Ser Ala Pro Ser Ser Ser Gly Ser Pro Leu 
385                 390                 395                 400 


Tyr Glu Gly Ala Ala Ala Ala Thr Asp Ile Val Asp Ser Gln Tyr Asp 
                405                 410                 415     


Ala Ala Ala Gln Gly Arg Leu Ile Ala Ser Trp Thr Pro Val Ser Pro 
            420                 425                 430         


Pro Ser Met 
        435 


<210>  8
<211>  1308
<212>  DNA
<213>  Artificial organism

<220>
<223>  Artificial sequence


<220>
<221>  isoform
<222>  (1)..(1308)
<223>  Coding sequence encoding Brachyury protein Isoform 1 with L254V

<400>  8
atgagctccc ctggcaccga gagcgcggga aagagcctgc agtaccgagt ggaccacctg       60

ctgagcgccg tggagaatga gctgcaggcg ggcagcgaga agggcgaccc cacagagcgc      120

gaactgcgcg tgggcctgga ggagagcgag ctgtggctgc gcttcaagga gctcaccaat      180

gagatgatcg tgaccaagaa cggcaggagg atgtttccgg tgctgaaggt gaacgtgtct      240

ggcctggacc ccaacgccat gtactccttc ctgctggact tcgtggcggc ggacaaccac      300

cgctggaagt acgtgaacgg ggaatgggtg ccggggggca agccggagcc gcaggcgccc      360

agctgcgtct acatccaccc cgactcgccc aacttcgggg cccactggat gaaggctccc      420

gtctccttca gcaaagtcaa gctcaccaac aagctcaacg gagggggcca gatcatgctg      480

aactccttgc ataagtatga gcctcgaatc cacatagtga gagttggggg tccacagcgc      540

atgatcacca gccactgctt ccctgagacc cagttcatag cggtgactgc ttatcagaac      600

gaggagatca cagctcttaa aattaagtac aatccatttg caaaggcttt ccttgatgca      660

aaggaaagaa gtgatcacaa agagatgatg gaggaacccg gagacagcca gcaacctggg      720

tactcccaat gggggtggct tcttcctgga accagcaccg tttgtccacc tgcaaatcct      780

catcctcagt ttggaggtgc cctctccctc ccctccacgc acagctgtga caggtaccca      840

accctgagga gccaccggtc ctcaccctac cccagcccct atgctcatcg gaacaattct      900

ccaacctatt ctgacaactc acctgcatgt ttatccatgc tgcaatccca tgacaattgg      960

tccagccttg gaatgcctgc ccatcccagc atgctccccg tgagccacaa tgccagccca     1020

cctaccagct ccagtcagta ccccagcctg tggtctgtga gcaacggcgc cgtcaccccg     1080

ggctcccagg cagcagccgt gtccaacggg ctgggggccc agttcttccg gggctccccc     1140

gcgcactaca cacccctcac ccatccggtc tcggcgccct cttcctcggg atccccactg     1200

tacgaagggg cggccgcggc cacagacatc gtggacagcc agtacgacgc cgcagcccaa     1260

ggccgcctca tagcctcatg gacacctgtg tcgccacctt ccatgtga                  1308


<210>  9
<211>  449
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  synthetic
<222>  (1)..(449)
<223>  Brachyury-I3 fusion protein

<400>  9

Met Lys Asn Asn Leu Tyr Glu Glu Lys Met Asn Met Ser Lys Lys Ser 
1               5                   10                  15      


Ser Pro Gly Thr Glu Ser Ala Gly Lys Ser Leu Gln Tyr Arg Val Asp 
            20                  25                  30          


His Leu Leu Ser Ala Val Glu Asn Glu Leu Gln Ala Gly Ser Glu Lys 
        35                  40                  45              


Gly Asp Pro Thr Glu Arg Glu Leu Arg Val Gly Leu Glu Glu Ser Glu 
    50                  55                  60                  


Leu Trp Leu Arg Phe Lys Glu Leu Thr Asn Glu Met Ile Val Thr Lys 
65                  70                  75                  80  


Asn Gly Arg Arg Met Phe Pro Val Leu Lys Val Asn Val Ser Gly Leu 
                85                  90                  95      


Asp Pro Asn Ala Met Tyr Ser Phe Leu Leu Asp Phe Val Ala Ala Asp 
            100                 105                 110         


Asn His Arg Trp Lys Tyr Val Asn Gly Glu Trp Val Pro Gly Gly Lys 
        115                 120                 125             


Pro Glu Pro Gln Ala Pro Ser Cys Val Tyr Ile His Pro Asp Ser Pro 
    130                 135                 140                 


Asn Phe Gly Ala His Trp Met Lys Ala Pro Val Ser Phe Ser Lys Val 
145                 150                 155                 160 


Lys Leu Thr Asn Lys Leu Asn Gly Gly Gly Gln Ile Met Leu Asn Ser 
                165                 170                 175     


Leu His Lys Tyr Glu Pro Arg Ile His Ile Val Arg Val Gly Gly Pro 
            180                 185                 190         


Gln Arg Met Ile Thr Ser His Cys Phe Pro Glu Thr Gln Phe Ile Ala 
        195                 200                 205             


Val Thr Ala Tyr Gln Asn Glu Glu Ile Thr Ala Leu Lys Ile Lys Tyr 
    210                 215                 220                 


Asn Pro Phe Ala Lys Ala Phe Leu Asp Ala Lys Glu Arg Ser Asp His 
225                 230                 235                 240 


Lys Glu Met Met Glu Glu Pro Gly Asp Ser Gln Gln Pro Gly Tyr Ser 
                245                 250                 255     


Gln Trp Gly Trp Leu Leu Pro Gly Thr Ser Thr Val Cys Pro Pro Ala 
            260                 265                 270         


Asn Pro His Pro Gln Phe Gly Gly Ala Leu Ser Leu Pro Ser Thr His 
        275                 280                 285             


Ser Cys Asp Arg Tyr Pro Thr Leu Arg Ser His Arg Ser Ser Pro Tyr 
    290                 295                 300                 


Pro Ser Pro Tyr Ala His Arg Asn Asn Ser Pro Thr Tyr Ser Asp Asn 
305                 310                 315                 320 


Ser Pro Ala Cys Leu Ser Met Leu Gln Ser His Asp Asn Trp Ser Ser 
                325                 330                 335     


Leu Gly Met Pro Ala His Pro Ser Met Leu Pro Val Ser His Asn Ala 
            340                 345                 350         


Ser Pro Pro Thr Ser Ser Ser Gln Tyr Pro Ser Leu Trp Ser Val Ser 
        355                 360                 365             


Asn Gly Ala Val Thr Pro Gly Ser Gln Ala Ala Ala Val Ser Asn Gly 
    370                 375                 380                 


Leu Gly Ala Gln Phe Phe Arg Gly Ser Pro Ala His Tyr Thr Pro Leu 
385                 390                 395                 400 


Thr His Pro Val Ser Ala Pro Ser Ser Ser Gly Ser Pro Leu Tyr Glu 
                405                 410                 415     


Gly Ala Ala Ala Ala Thr Asp Ile Val Asp Ser Gln Tyr Asp Ala Ala 
            420                 425                 430         


Ala Gln Gly Arg Leu Ile Ala Ser Trp Thr Pro Val Ser Pro Pro Ser 
        435                 440                 445             


Met 
    


<210>  10
<211>  1350
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  fusion protein
<222>  (1)..(1350)
<223>  Coding sequence encoding I3 Brachyury fusion protein of SEQ ID NO
       9

<400>  10
atgaaaaata acttgtatga agaaaaaatg aacatgagta agaaaagctc ccctggcacc       60

gagagcgcgg gaaagagcct gcagtaccga gtggaccacc tgctgagcgc cgtggagaat      120

gagctgcagg cgggcagcga gaagggcgac cccacagagc gcgaactgcg cgtgggcctg      180

gaggagagcg agctgtggct gcgcttcaag gagctcacca atgagatgat cgtgaccaag      240

aacggcagga ggatgtttcc ggtgctgaag gtgaacgtgt ctggcctgga ccccaacgcc      300

atgtactcct tcctgctgga cttcgtggcg gcggacaacc accgctggaa gtacgtgaac      360

ggggaatggg tgccgggggg caagccggag ccgcaggcgc ccagctgcgt ctacatccac      420

cccgactcgc ccaacttcgg ggcccactgg atgaaggctc ccgtctcctt cagcaaagtc      480

aagctcacca acaagctcaa cggagggggc cagatcatgc tgaactcctt gcataagtat      540

gagcctcgaa tccacatagt gagagttggg ggtccacagc gcatgatcac cagccactgc      600

ttccctgaga cccagttcat agcggtgact gcttatcaga acgaggagat cacagctctt      660

aaaattaagt acaatccatt tgcaaaggct ttccttgatg caaaggaaag aagtgatcac      720

aaagagatga tggaggaacc cggagacagc cagcaacctg ggtactccca atgggggtgg      780

cttcttcctg gaaccagcac cgtttgtcca cctgcaaatc ctcatcctca gtttggaggt      840

gccctctccc tcccctccac gcacagctgt gacaggtacc caaccctgag gagccaccgg      900

tcctcaccct accccagccc ctatgctcat cggaacaatt ctccaaccta ttctgacaac      960

tcacctgcat gtttatccat gctgcaatcc catgacaatt ggtccagcct tggaatgcct     1020

gcccatccca gcatgctccc cgtgagccac aatgccagcc cacctaccag ctccagtcag     1080

taccccagcc tgtggtctgt gagcaacggc gccgtcaccc cgggctccca ggcagcagcc     1140

gtgtccaacg ggctgggggc ccagttcttc cggggctccc ccgcgcacta cacacccctc     1200

acccatccgg tctcggcgcc ctcttcctcg ggatccccac tgtacgaagg ggcggccgcg     1260

gccacagaca tcgtggacag ccagtacgac gccgcagccc aaggccgcct catagcctca     1320

tggacacctg tgtcgccacc ttccatgtga                                      1350


<210>  11
<211>  261
<212>  PRT
<213>  Homo sapiens


<220>
<221>  hCD40L
<222>  (1)..(261)
<223>  hCD40L from NCBI RefSeq NP_000065.1.

<400>  11

Met Ile Glu Thr Tyr Asn Gln Thr Ser Pro Arg Ser Ala Ala Thr Gly 
1               5                   10                  15      


Leu Pro Ile Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Ala Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe Val Lys 
                85                  90                  95      


Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Ser Phe Glu 
            100                 105                 110         


Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
        115                 120                 125             


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
    130                 135                 140                 


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
145                 150                 155                 160 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
                165                 170                 175     


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
            180                 185                 190         


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
        195                 200                 205             


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
    210                 215                 220                 


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
225                 230                 235                 240 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
                245                 250                 255     


Gly Leu Leu Lys Leu 
            260     


<210>  12
<211>  789
<212>  DNA
<213>  Homo sapiens


<220>
<221>  hCD40L
<222>  (1)..(789)
<223>  hCD40L from NCBI RefSeq NP_000065.1.

<400>  12
atgatcgaga catacaacca gacaagccct agaagcgccg ccacaggact gcctatcagc       60

atgaagatct tcatgtacct gctgaccgtg ttcctgatca cccagatgat cggcagcgcc      120

ctgtttgccg tgtacctgca cagacggctg gacaagatcg aggacgagag aaacctgcac      180

gaggacttcg tgttcatgaa gaccatccag cggtgcaaca ccggcgagag aagtctgagc      240

ctgctgaact gcgaggaaat caagagccag ttcgagggct tcgtgaagga catcatgctg      300

aacaaagagg aaacgaagaa agagaactcc ttcgagatgc agaagggcga ccagaatcct      360

cagatcgccg ctcacgtgat cagcgaggcc agcagcaaga caacaagcgt gctgcagtgg      420

gccgagaagg gctactacac catgagcaac aacctggtca ccctggagaa cggcaagcag      480

ctgacagtga agcggcaggg cctgtactac atctacgccc aagtgacctt ctgcagcaac      540

agagaggcca gctctcaggc tcctttcatc gccagcctgt gcctgaagtc tcctggcaga      600

ttcgagcgga ttctgctgag agccgccaac acacacagca gcgccaaacc ttgtggccag      660

cagtctattc acctcggcgg agtgtttgag ctgcagcctg gcgcaagcgt gttcgtgaat      720

gtgacagacc ctagccaggt gtcccacggc accggcttta catctttcgg actgctgaag      780

ctgtgatga                                                              789


<210>  13
<211>  1145
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  synthetic
<222>  (1)..(1145)
<223>  Synthetic Her2 v2 amino acid sequence

<400>  13

Met Glu Leu Ala Ala Leu Cys Arg Trp Gly Leu Leu Leu Ala Leu Leu 
1               5                   10                  15      


Pro Pro Gly Ala Ala Ser Thr Gln Val Cys Thr Gly Thr Asp Met Lys 
            20                  25                  30          


Leu Arg Leu Pro Ala Ser Pro Glu Thr His Leu Asp Met Leu Arg His 
        35                  40                  45              


Leu Tyr Gln Gly Cys Gln Val Val Gln Gly Asn Leu Glu Leu Thr Tyr 
    50                  55                  60                  


Leu Pro Thr Asn Ala Ser Leu Ser Phe Leu Gln Asp Ile Gln Glu Val 
65                  70                  75                  80  


Gln Gly Tyr Val Leu Ile Ala His Asn Gln Val Arg Gln Val Pro Leu 
                85                  90                  95      


Gln Arg Leu Arg Ile Val Arg Gly Thr Gln Leu Phe Glu Asp Asn Tyr 
            100                 105                 110         


Ala Leu Ala Val Leu Asp Asn Gly Asp Pro Leu Asn Asn Thr Thr Pro 
        115                 120                 125             


Val Thr Gly Ala Ser Pro Gly Gly Leu Arg Glu Leu Gln Leu Arg Ser 
    130                 135                 140                 


Leu Thr Glu Ile Leu Lys Gly Gly Val Leu Ile Gln Arg Asn Pro Gln 
145                 150                 155                 160 


Leu Cys Tyr Gln Asp Thr Ile Leu Trp Lys Asp Ile Phe His Lys Asn 
                165                 170                 175     


Asn Gln Leu Ala Leu Thr Leu Ile Asp Thr Asn Arg Ser Arg Ala Cys 
            180                 185                 190         


His Pro Cys Ser Pro Met Cys Lys Gly Ser Arg Cys Trp Gly Glu Ser 
        195                 200                 205             


Ser Glu Asp Cys Gln Ser Leu Thr Arg Thr Val Cys Ala Gly Gly Cys 
    210                 215                 220                 


Ala Arg Cys Lys Gly Pro Leu Pro Thr Asp Cys Cys His Glu Gln Cys 
225                 230                 235                 240 


Ala Ala Gly Cys Thr Gly Pro Lys His Ser Asp Cys Leu Ala Cys Leu 
                245                 250                 255     


His Phe Asn His Ser Gly Ile Cys Glu Leu Ala Cys Pro Ala Leu Val 
            260                 265                 270         


Thr Tyr Asn Thr Arg Thr Ala Lys Ser Met Pro Asn Pro Glu Gly Arg 
        275                 280                 285             


Tyr Thr Phe Gly Ala Ser Cys Val Thr Ala Cys Pro Tyr Asn Tyr Leu 
    290                 295                 300                 


Ser Thr Asp Ala Gly Ala Cys Thr Leu Val Cys Pro Ala Ala Asn Gln 
305                 310                 315                 320 


Glu Val Thr Ala Glu Asp Gly Thr Gln Arg Cys Glu Ala Cys Ser Lys 
                325                 330                 335     


Ala Cys Ala Arg Val Cys Tyr Gly Leu Gly Met Glu His Leu Arg Glu 
            340                 345                 350         


Val Arg Ala Val Thr Ser Ala Asn Ile Gln Glu Phe Ala Gly Cys Lys 
        355                 360                 365             


Lys Ile Phe Gly Ser Leu Ala Phe Leu Pro Glu Ser Phe Asp Gly Asp 
    370                 375                 380                 


Pro Ala Ser Asn Thr Ala Pro Leu Gln Pro Glu Gln Leu Gln Val Phe 
385                 390                 395                 400 


Glu Thr Leu Glu Glu Ile Thr Gly Tyr Leu Tyr Ile Ser Ala Trp Pro 
                405                 410                 415     


Asp Ser Leu Pro Asp Leu Ser Val Phe Gln Asn Leu Gln Val Ile Arg 
            420                 425                 430         


Gly Arg Ile Leu His Asn Gly Ala Tyr Ser Leu Thr Leu Gln Gly Leu 
        435                 440                 445             


Gly Ile Ser Trp Leu Gly Leu Arg Ser Leu Arg Glu Leu Gly Ser Gly 
    450                 455                 460                 


Leu Ala Leu Ile His His Asn Thr His Leu Cys Phe Val His Thr Val 
465                 470                 475                 480 


Pro Trp Asp Gln Leu Phe Arg Asn Pro His Gln Ala Leu Leu His Thr 
                485                 490                 495     


Ala Asn Arg Pro Glu Asp Glu Cys Val Gly Glu Gly Leu Ala Cys His 
            500                 505                 510         


Gln Leu Cys Ala Arg Gly His Cys Trp Gly Pro Gly Pro Thr Gln Cys 
        515                 520                 525             


Val Asn Cys Ser Gln Phe Leu Arg Gly Gln Glu Cys Val Glu Glu Cys 
    530                 535                 540                 


Arg Val Leu Gln Gly Leu Pro Arg Glu Tyr Val Asn Ala Arg His Cys 
545                 550                 555                 560 


Leu Pro Cys His Pro Glu Cys Gln Pro Gln Asn Gly Ser Val Thr Cys 
                565                 570                 575     


Phe Gly Pro Ala Ala Asp Gln Cys Val Ala Cys Ala His Tyr Lys Asp 
            580                 585                 590         


Pro Pro Ala Cys Val Ala Arg Cys Pro Ser Gly Val Lys Pro Asp Leu 
        595                 600                 605             


Ser Tyr Met Pro Ile Trp Ala Phe Pro Asp Glu Glu Gly Ala Cys Gln 
    610                 615                 620                 


Pro Cys Pro Ile Asn Cys Thr His Ser Cys Val Asp Leu Asp Asp Lys 
625                 630                 635                 640 


Gly Cys Pro Ala Glu Gln Arg Ala Ser Pro Leu Thr Ser Ile Ile Ser 
                645                 650                 655     


Ala Val Val Gly Ile Leu Leu Val Val Val Leu Gly Val Val Phe Gly 
            660                 665                 670         


Ile Leu Ile Lys Arg Arg Gln Gln Lys Ile Arg Lys Tyr Thr Met Arg 
        675                 680                 685             


Arg Leu Leu Gln Glu Thr Glu Leu Val Glu Pro Leu Thr Pro Ser Gly 
    690                 695                 700                 


Ala Met Pro Asn Gln Ala Gln Met Arg Ile Leu Lys Glu Thr Glu Leu 
705                 710                 715                 720 


Arg Lys Val Lys Val Leu Gly Ser Gly Ala Phe Gly Thr Val Tyr Lys 
                725                 730                 735     


Gly Ile Trp Ile Pro Asp Gly Glu Asn Val Lys Ile Pro Val Ala Ile 
            740                 745                 750         


Met Val Leu Arg Glu Asn Thr Ser Pro Lys Ala Asn Lys Glu Ile Leu 
        755                 760                 765             


Asp Glu Ala Tyr Val Met Ala Gly Val Gly Ser Pro Tyr Val Ser Arg 
    770                 775                 780                 


Leu Leu Gly Ile Cys Leu Thr Ser Thr Val Gln Leu Val Thr Gln Leu 
785                 790                 795                 800 


Met Pro Tyr Gly Cys Leu Leu Asp His Val Arg Glu Asn Arg Gly Arg 
                805                 810                 815     


Leu Gly Ser Gln Asp Leu Leu Asn Trp Cys Met Gln Ile Ala Lys Gly 
            820                 825                 830         


Met Ser Tyr Leu Glu Asp Val Arg Leu Val His Arg Asp Leu Ala Ala 
        835                 840                 845             


Arg Asn Val Leu Val Lys Ser Pro Asn His Val Lys Ile Thr Asp Phe 
    850                 855                 860                 


Gly Leu Ala Arg Leu Leu Asp Ile Asp Glu Thr Glu Tyr His Ala Asp 
865                 870                 875                 880 


Gly Gly Lys Val Pro Ile Lys Trp Met Ala Leu Glu Ser Ile Leu Arg 
                885                 890                 895     


Arg Arg Phe Thr His Gln Ser Asp Val Trp Ser Tyr Gly Val Thr Val 
            900                 905                 910         


Trp Glu Leu Met Thr Phe Gly Ala Lys Pro Tyr Asp Gly Ile Pro Ala 
        915                 920                 925             


Arg Glu Ile Pro Asp Leu Leu Glu Lys Gly Glu Arg Leu Pro Gln Pro 
    930                 935                 940                 


Pro Ile Cys Thr Ile Asp Val Tyr Met Ile Met Val Lys Cys Trp Met 
945                 950                 955                 960 


Ile Asp Ser Glu Cys Arg Pro Arg Phe Arg Glu Leu Val Ser Glu Phe 
                965                 970                 975     


Ser Arg Met Ala Arg Asp Pro Gln Arg Phe Val Val Ile Gln Asn Glu 
            980                 985                 990         


Asp Leu Gly Pro Ala Ser Pro Leu  Asp Ser Thr Phe Tyr  Arg Ser Leu 
        995                 1000                 1005             


Leu Glu  Asp Asp Asp Met Gly  Asp Leu Val Asp Ala  Glu Glu Ala 
    1010                 1015                 1020             


Leu Val  Pro Gln Gln Gly Phe  Phe Cys Pro Asp Pro  Ala Pro Gly 
    1025                 1030                 1035             


Ala Gly  Gly Met Val His His  Arg His Arg Ser Ser  Ser Thr Arg 
    1040                 1045                 1050             


Ser Gly  Gly Gly Asp Leu Thr  Leu Gly Leu Glu Pro  Ser Glu Glu 
    1055                 1060                 1065             


Glu Ala  Pro Arg Ser Pro Leu  Ala Pro Ser Glu Gly  Ala Gly Ser 
    1070                 1075                 1080             


Asp Val  Phe Asp Gly Asp Leu  Gly Met Gly Ala Ala  Lys Gly Leu 
    1085                 1090                 1095             


Gln Ser  Leu Pro Thr His Asp  Pro Ser Pro Leu Gln  Arg Tyr Ser 
    1100                 1105                 1110             


Glu Asp  Pro Thr Val Pro Leu  Pro Ser Glu Thr Asp  Gly Tyr Val 
    1115                 1120                 1125             


Ala Pro  Leu Thr Cys Ser Pro  Gln Pro Glu Leu Gly  Leu Asp Val 
    1130                 1135                 1140             


Pro Val  
    1145 


<210>  14
<211>  3441
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  synthetic
<222>  (1)..(3441)
<223>  Synthetic Her2 v2 nucleotide sequence

<400>  14
atggaactgg ctgctctgtg tagatgggga ctgctgcttg ctctgttgcc tcctggagct       60

gcttctaccc aagtgtgcac aggcaccgac atgaagctga gactgcctgc ttctcctgag      120

acacacctgg acatgctgag acacctgtac cagggatgtc aggtggtgca gggaaatctg      180

gaactgacct acctgcctac caacgccagc ctgagctttc tgcaggacat ccaagaggtg      240

cagggatacg tgctgatcgc tcacaatcaa gtgagacagg tgccactgca gaggctgaga      300

atcgttagag gcacccagct gttcgaggac aactatgctc tggctgtgct ggacaatggc      360

gaccctctga acaacaccac acctgtgaca ggagcttctc ctggtggact gagagaactg      420

cagctgagaa gcctgaccga gatcctgaaa ggaggagtgc tgatccagcg gaaccctcag      480

ctgtgctacc aggacaccat cctgtggaag gacatcttcc acaagaacaa ccagctggct      540

ctgacactga tcgacaccaa cagaagcaga gcctgccatc cttgctctcc catgtgcaag      600

ggctctagat gttggggaga gagcagcgag gattgccaga gcctgaccag aacagtgtgt      660

gctggaggat gtgccagatg caaaggacct ctgcctaccg actgctgcca cgagcaatgt      720

gcagctggat gtacaggacc aaagcactct gattgcctgg cctgcctgca cttcaaccac      780

tctggaatct gcgagctcgc ctgtcctgct ctggtcacct acaacacacg gaccgccaag      840

agcatgccta atcctgaagg cagatacacc tttggagcca gctgtgtgac agcctgtcct      900

tacaactacc tgagcaccga cgctggagcc tgcacactcg tttgtcctgc tgccaatcaa      960

gaagtgacgg ccgaggacgg cacccagaga tgcgaggcct gtagcaaggc ttgcgctaga     1020

gtgtgttacg gactcggcat ggaacacctg agagaagtga gagccgtgac cagtgccaac     1080

atccaagagt ttgctggctg caagaagatc tttggcagcc tcgccttcct gcctgagagc     1140

ttcgatggcg atcctgccag caatactgct cctctgcagc ctgaacagct ccaggtgttc     1200

gagacactgg aagagatcac aggctacctg tacatcagcg catggccaga cagcctgcct     1260

gacctgtccg tgttccagaa cctgcaagtg atcagaggca gaatcctgca caacggagcc     1320

tattctctga ccctgcaagg cctgggaatc agctggctgg gactgagatc cctgagagag     1380

cttggatctg gcctggctct gatccaccac aatacccacc tgtgcttcgt gcacaccgtg     1440

ccttgggacc agctgtttcg gaatcctcat caggctctgc tgcacacagc caacagacct     1500

gaggatgagt gtgttggcga aggcctggct tgtcaccagc tctgtgctag aggacactgt     1560

tggggacctg gacctacaca gtgtgtgaac tgtagccagt tcctgagagg ccaagaatgc     1620

gtggaagagt gtagagttct gcagggactg cctcgcgagt acgtgaacgc tagacactgt     1680

ctgccttgtc atcccgagtg ccagcctcag aatggcagcg tgacatgttt tggaccagct     1740

gccgatcagt gcgtggcctg tgctcactat aaggaccctc cagcctgcgt ggccagatgt     1800

cctagcggag tgaagcctga cctgagctac atgcccatct gggcatttcc agatgaggaa     1860

ggagcttgcc agccttgtcc tatcaactgc acccacagct gcgtggacct ggacgataag     1920

ggatgtccag ccgagcagag agcctctcca ctgacctcta tcatctctgc cgtcgtgggc     1980

atcctgctgg tggtggttct gggagttgtg ttcggcatcc tgatcaagag acggcagcag     2040

aagatccgga agtacaccat gcggagactg ctgcaagaga ctgagctggt ggaacctctg     2100

acacccagcg gagctatgcc taaccaggct cagatgcgga ttctgaaaga aaccgagctg     2160

cggaaagtga aggtgctcgg ctctggagcc tttggcacag tgtacaaagg catctggatc     2220

cctgacggag agaacgtgaa gattcctgtg gccatcatgg tgctgagaga gaacacaagt     2280

cccaaggcca acaaagagat cctggacgag gcctacgtga tggctggtgt tggcagccct     2340

tatgtgtcta gactgctggg catctgtctg accagcaccg tgcagctggt cactcagctg     2400

atgccttacg gctgcctgct ggatcacgtg agagagaata gaggcagact gggctctcag     2460

gacctgctga actggtgcat gcagatcgcc aagggcatga gctacctcga ggatgtgaga     2520

ctggtccaca gagatctggc tgccagaaac gtgctcgtga agtctcctaa ccacgtgaag     2580

atcaccgact tcggactggc taggctgctg gatatcgacg agacagagta ccacgctgat     2640

ggaggcaagg tgcccatcaa gtggatggct ctggaatcca tcctgagacg gagattcacc     2700

caccagtccg atgtgtggtc ttacggagtg acagtgtggg agctgatgac cttcggagcc     2760

aagccttacg acggcatccc tgccagagag atcccagatc tgctggaaaa gggagagaga     2820

ctgcctcagc ctcctatctg caccatcgac gtgtacatga ttatggtcaa gtgttggatg     2880

atcgacagcg agtgcagacc cagattcaga gaactggtgt ccgagttctc tcggatggcc     2940

agagatcctc agagattcgt ggtcatccag aacgaggatc tgggacctgc cagccctctg     3000

gacagcacct tctacagatc cctgctggaa gatgacgaca tgggtgacct ggtggacgct     3060

gaagaagctc tggttcctca gcagggcttc ttctgccctg atcctgctcc aggagcaggt     3120

ggaatggtgc atcacagaca cagaagctcc agcaccagaa gcggaggcgg agatctgaca     3180

ctgggactcg agccatctga ggaagaggct cctagatctc ctctggctcc ttctgaagga     3240

gctggaagcg acgttttcga cggagatctt ggaatgggag ctgccaaagg actccagtct     3300

ctgcccacac acgacccatc tccactgcag agatacagcg aggaccctac cgtgcctctg     3360

ccaagcgaga cagatggata tgtggcacct ctgacctgct ctcctcagcc agaactggga     3420

cttgatgtgc ctgtttgatg a                                               3441


<210>  15
<211>  205
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  synthetic
<222>  (1)..(205)
<223>  Synthetic Twist amino acid sequence

<400>  15

Met Gln Asp Val Ser Ser Ser Pro Val Ser Pro Ala Asp Asp Ser Leu 
1               5                   10                  15      


Ser Asn Ser Glu Glu Glu Pro Asp Arg Gln Gln Pro Ala Ser Gly Lys 
            20                  25                  30          


Arg Gly Ala Arg Lys Arg Arg Ser Ser Arg Arg Ser Ala Gly Gly Ser 
        35                  40                  45              


Ala Gly Pro Gly Gly Ala Thr Gly Gly Gly Ile Gly Gly Gly Asp Glu 
    50                  55                  60                  


Pro Gly Ser Pro Ala Gln Gly Lys Arg Gly Lys Lys Ser Ala Gly Gly 
65                  70                  75                  80  


Gly Gly Gly Gly Gly Ala Gly Gly Gly Gly Gly Gly Gly Gly Gly Ser 
                85                  90                  95      


Ser Ser Gly Gly Gly Ser Pro Gln Ser Tyr Glu Glu Leu Gln Thr Gln 
            100                 105                 110         


Arg Val Met Ala Asn Val Arg Glu Arg Gln Arg Thr Gln Ser Leu Asn 
        115                 120                 125             


Glu Ala Phe Ala Ala Leu Arg Lys Ile Ile Pro Thr Leu Pro Ser Asp 
    130                 135                 140                 


Lys Leu Ser Lys Ile Gln Thr Leu Lys Leu Ala Ala Arg Tyr Ile Asp 
145                 150                 155                 160 


Phe Leu Tyr Gln Val Leu Gln Ser Asp Glu Leu Asp Ser Lys Met Ala 
                165                 170                 175     


Ser Cys Ser Tyr Val Ala His Glu Arg Leu Ser Tyr Ala Phe Ser Val 
            180                 185                 190         


Trp Arg Met Glu Gly Ala Trp Ser Met Ser Ala Ser His 
        195                 200                 205 


<210>  16
<211>  618
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  synthetic
<222>  (1)..(618)
<223>  Synthetic Twist nucleotide sequence

<400>  16
atgcaggacg tgtccagcag ccctgtgtct cctgccgacg acagcctgag caacagcgag       60

gaagaacccg acagacagca gcccgcctct ggcaagagag gcgccagaaa gagaagaagc      120

tccagaagaa gcgctggcgg ctctgctgga cctggcggag ctacaggcgg aggaattgga      180

ggcggagatg agcctggctc tccagcccag ggcaagaggg gcaagaaatc tgctggcgga      240

ggcggcggag gaggagctgg aggcggagga ggaggcggcg gaggatcaag ttctggcgga      300

ggaagccctc agagctacga ggaactgcag acccagcgcg tgatggccaa cgtgcgcgag      360

agacagagaa cccagagcct gaacgaggcc ttcgccgccc tgagaaagat catccccacc      420

ctgcccagcg acaagctgag caagatccag accctgaagc tggccgccag atatatcgac      480

ttcctgtatc aagtgctgca gagcgacgag ctggacagca agatggccag ctgctcctac      540

gtggcccacg agagactgag ctacgccttc agcgtgtggc ggatggaagg cgcctggtct      600

atgagcgcca gccactga                                                    618


<210>  17
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence


<220>
<221>  synthetic
<222>  (1)..(260)
<223>  Synthetic murine CD40L amino acid sequence

<400>  17

Met Ile Glu Thr Tyr Ser Gln Pro Ser Pro Arg Ser Val Ala Thr Gly 
1               5                   10                  15      


Leu Pro Ala Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Val Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Val Glu Glu Glu Val Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Ile Lys Lys Leu Lys Arg Cys Asn Lys Gly Glu Gly Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Met Arg Arg Gln Phe Glu Asp Leu Val Lys 
                85                  90                  95      


Asp Ile Thr Leu Asn Lys Glu Glu Lys Lys Glu Asn Ser Phe Glu Met 
            100                 105                 110         


Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser Glu 
        115                 120                 125             


Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr 
    130                 135                 140                 


Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu 
145                 150                 155                 160 


Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe 
                165                 170                 175     


Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu 
            180                 185                 190         


Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala 
        195                 200                 205             


Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu 
    210                 215                 220                 


Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val 
225                 230                 235                 240 


Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe Gly 
                245                 250                 255     


Leu Leu Lys Leu 
            260 


<210>  18
<211>  786
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial sequence


<220>
<221>  synthetic
<222>  (1)..(786)
<223>  Synthetic murine CD40L nucleotide sequence

<400>  18
atgatcgaga catacagcca gcccagcccc agaagcgtgg ccacaggact gcctgccagc       60

atgaagatct ttatgtacct gctgaccgtg ttcctgatca cccagatgat cggcagcgtg      120

ctgttcgccg tgtacctgca cagacggctg gacaaggtgg aagaggaagt gaacctgcac      180

gaggacttcg tgttcatcaa gaaactgaag cggtgcaaca agggcgaggg cagcctgagc      240

ctgctgaact gcgaggaaat gagaaggcag ttcgaggacc tcgtgaagga catcaccctg      300

aacaaagagg aaaagaaaga aaactccttc gagatgcaga ggggcgacga ggaccctcag      360

atcgctgctc acgtggtgtc cgaggccaac agcaacgccg cttctgtgct gcagtgggcc      420

aagaaaggct actacaccat gaagtccaac ctcgtgatgc tggaaaacgg caagcagctg      480

acagtgaagc gcgagggcct gtactatgtg tacacccaag tgacattctg cagcaacaga      540

gagcccagca gccagaggcc cttcatcgtg ggactgtggc tgaagcctag cagcggcagc      600

gagagaatcc tgctgaaggc cgccaacacc cacagcagct ctcagctgtg cgagcagcag      660

agcgtgcacc tgggcggagt gttcgagctg caagctggcg cctccgtgtt cgtgaacgtg      720

acagaggcca gccaagtgat ccacagagtg ggcttcagca gctttggact gctgaaactg      780

taatga                                                                 786


