                         SEQUENCE LISTING

<110>  Massachusetts Institute of Technology
 
<120>  ENGINEERED DCAS9 WITH REDUCED TOXICITY AND ITS USE IN GENETIC 
       CIRCUITS

<130>  M0656.70412WO00

<140>  Not yet assigned
<141>  Concurrently herewith

<150>  US 62/735,877
<151>  2018-09-25

<160>  139   

<170>  PatentIn version 3.5

<210>  1
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  1
ataatacccc tactaggagt gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  2
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  2
tgtctacccg aaggcggcgt atgatacgaa acgtaccgta tcgttaaggt acatggttta       60

caccaactcc tagtaggggt attatgctag c                                      91


<210>  3
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  3
ataataccgc actctcctag gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  4
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  4
tttaggtttg ccgacgcccg atgatacgaa acgtaccgta tcgttaaggt ggtttgttta       60

caccactagg agagtgcggt attatgctag c                                      91


<210>  5
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  5
ataataccct agggacccct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  6
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  6
agacaacctt gacatggggc atgatacgaa acgtaccgta tcgttaaggt atacacttta       60

caccaagggg tccctagggt attatgctag c                                      91


<210>  7
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  7
ataataccag tcctagccta gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  8
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  8
aagtccttat ctgcgcaatc atgatacgaa acgtaccgta tcgttaaggt cgatgattta       60

caccataggc taggactggt attatgctag c                                      91


<210>  9
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  9
ataataccag gtcctaagtg gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  10
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  10
tctatacatc cgaagtcgag atgatacgaa acgtaccgta tcgttaaggt tacagattta       60

caccacactt aggacctggt attatgctag c                                      91


<210>  11
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  11
ataataccga ccctccctct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  12
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  12
cacatcaatc gctaggtggc atgatacgaa acgtaccgta tcgttaaggt ccggcgttta       60

caccaagagg gagggtcggt attatgctag c                                      91


<210>  13
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  13
ataatacctg tcctaacact gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  14
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  14
gattcgaata atctcgagcc atgatacgaa acgtaccgta tcgttaaggt tgctatttta       60

caccaagtgt taggacaggt attatgctag c                                      91


<210>  15
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  15
ataatacccc ctctagctag gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  16
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  16
gtctcgaaca cctatcagtt atgatacgaa acgtaccgta tcgttaaggt ctcgacttta       60

caccactagc tagagggggt attatgctag c                                      91


<210>  17
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  17
ataatacccg tgtgacccgt gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  18
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  18
tccgaatgac atgcgtctcc atgatacgaa acgtaccgta tcgttaaggt acagccttta       60

caccaacggg tcacacgggt attatgctag c                                      91


<210>  19
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  19
ataataccgc actctcctag gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  20
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  20
ccaaacgcca tatctttgac atgatacgaa acgtaccgta tcgttaaggt acaaccttta       60

caccactagg agagtgcggt attatgctag c                                      91


<210>  21
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  21
ataataccct ctagtctaga gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  22
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  22
acaaagccta ttacgatgac atgatacgaa acgtaccgta tcgttaaggt tagtaattta       60

caccatctag actagagggt attatgctag c                                      91


<210>  23
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  23
ataataccct cctagtctag gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  24
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  24
gtgagagtac tttatacgct atgatacgaa acgtaccgta tcgttaaggt tttgacttta       60

caccactaga ctaggagggt attatgctag c                                      91


<210>  25
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  25
ataataccac tactagagtg gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  26
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  26
tggtcgcagc agagcgagga atgatacgaa acgtaccgta tcgttaaggt cgaagtttta       60

caccacactc tagtagtggt attatgctag c                                      91


<210>  27
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  27
ataatacctg tcctagaggt gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  28
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  28
ccttgaggag ctggttgtaa atgatacgaa acgtaccgta tcgttaaggt ctgggcttta       60

caccaacctc taggacaggt attatgctag c                                      91


<210>  29
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  29
ataataccag tgtacctagt gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  30
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  30
tttctcagcg taatcgttcg atgatacgaa acgtaccgta tcgttaaggt accgaattta       60

caccaactag gtacactggt attatgctag c                                      91


<210>  31
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  31
ataataccga cataggatct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  32
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  32
cgagattccc ttatcctttt atgatacgaa acgtaccgta tcgttaaggt atgcacttta       60

caccaagatc ctatgtcggt attatgctag c                                      91


<210>  33
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  33
ataataccgg gagtcctata gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  34
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  34
gatcgcctca ctttgaaatt atgatacgaa acgtaccgta tcgttaaggt gcggccttta       60

caccatatag gactcccggt attatgctag c                                      91


<210>  35
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  35
ataataccct agggacccct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  36
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  36
aatagataca gttaggtttg atgatacgaa acgtaccgta tcgttaaggt gaccagttta       60

caccaagggg tccctagggt attatgctag c                                      91


<210>  37
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  37
ataataccgt atgggactct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  38
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  38
tgtagaggtt aagcaggtca atgatacgaa acgtaccgta tcgttaaggt catgacttta       60

caccaagagt cccatacggt attatgctag c                                      91


<210>  39
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  39
ataataccag actctagggt gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  40
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  40
ttaaccactg taagaaagtt atgatacgaa acgtaccgta tcgttaaggt ctcgtattta       60

caccaaccct agagtctggt attatgctag c                                      91


<210>  41
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  41
ataatacctc ctactagact gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  42
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  42
gccttgagtt aggctctctc atgatacgaa acgtaccgta tcgttaaggt gcatatttta       60

caccaagtct agtaggaggt attatgctag c                                      91


<210>  43
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  43
ataatacctc tagagtccct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  44
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  44
ctccgtcgga gttgacgtcg atgatacgaa acgtaccgta tcgttaaggt tcggatttta       60

caccaaggga ctctagaggt attatgctag c                                      91


<210>  45
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  45
ataataccac ccctagggac gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  46
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  46
acgactaccg cagtgcagta atgatacgaa acgtaccgta tcgttaaggt tttaatttta       60

caccagtccc taggggtggt attatgctag c                                      91


<210>  47
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  47
ataataccga cttggacccc gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  48
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  48
ccctgtcgtt agtctccgag atgatacgaa acgtaccgta tcgttaaggt tttaggttta       60

caccaggggt ccaagtcggt attatgctag c                                      91


<210>  49
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  49
ataataccag gacctagtat gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  50
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  50
ctgttccgcg tcacatcaac atgatacgaa acgtaccgta tcgttaaggt ggagtattta       60

caccaatact aggtcctggt attatgctag c                                      91


<210>  51
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  51
ataataccag tcctacctct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  52
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  52
caactcgtga tatccgcctg atgatacgaa acgtaccgta tcgttaaggt gctcgcttta       60

caccaagagg taggactggt attatgctag c                                      91


<210>  53
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  53
ataatacccc ctctagctag gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  54
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  54
acggagtctg agacccggcg atgatacgaa acgtaccgta tcgttaaggt aagaccttta       60

caccactagc tagagggggt attatgctag c                                      91


<210>  55
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  55
ataataccac tagacctagt gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  56
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  56
ggttaagttg aacctccgat atgatacgaa acgtaccgta tcgttaaggt cacttcttta       60

caccaactag gtctagtggt attatgctag c                                      91


<210>  57
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  57
ataataccac tagtccaagg gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  58
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  58
ctcagaagct accaatgttt atgatacgaa acgtaccgta tcgttaaggt ttaaggttta       60

caccaccttg gactagtggt attatgctag c                                      91


<210>  59
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  59
ataataccgt ctaggacccc gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  60
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gate

<400>  60
ggttctatat ctctaggggt atgatacgaa acgtaccgta tcgttaaggt cgacatttta       60

caccaggggt cctagacggt attatgctag c                                      91


<210>  61
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  61
tccgaatgac atgcgtctcc cgctccaaca ccgttggttg aacagccttt acaccaacgg       60

gtcacacggg tattatgcta gc                                                82


<210>  62
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  62
tccgaatgac atgcgtctcc ggtgttggag cggttggttg aacagccttt acaccaacgg       60

gtcacacggg tattatgcta gc                                                82


<210>  63
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  63
tccgaatgac atgcgtctcc ggtgttggag cgtttacacc aacgggtcac acgggtatta       60

tgctagc                                                                 67


<210>  64
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  64
tccgaatgac atgcgtctcc ggtgttggag cgcctttaca ccaacgggtc acacgggtat       60

tatgctagc                                                               69


<210>  65
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  65
tccgaatgac atgcgtctcc ggtgttggag cgagccttta caccaacggg tcacacgggt       60

attatgctag c                                                            71


<210>  66
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  66
tccgaatgac atgcgtctcc ggtgttggag cgacagcctt tacaccaacg ggtcacacgg       60

gtattatgct agc                                                          73


<210>  67
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  67
tccgaatgac atgcgtctcc ggtgttggag cggaacagcc tttacaccaa cgggtcacac       60

gggtattatg ctagc                                                        75


<210>  68
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  68
tccgaatgac atgcgtctcc atgatacgaa acgtaccgta tcgttaaggt cctttacacc       60

aacgggtcac acgggtatta tgctagc                                           87


<210>  69
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  69
tccgaatgac atgcgtctcc atgatacgaa acgtaccgta tcgttaaggt agcctttaca       60

ccaacgggtc acacgggtat tatgctagc                                         89


<210>  70
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  70
tccgaatgac atgcgtctcc atgatacgaa acgtaccgta tcgttaaggt cagcctttac       60

accaacgggt cacacgggta ttatgctagc                                        90


<210>  71
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  71
tccgaatgac atgcgtctcc atgatacgaa acgtaccgta tcgttaaggt acagccttta       60

caccaacggg tcacacgggt attatgctag c                                      91


<210>  72
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  72
tccgaatgac atgcgtctcc atgatacgaa acgtaccgta tcgttaaggt aacagccttt       60

acaccaacgg gtcacacggg tattatgcta gc                                     92


<210>  73
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  73
tccgaatgac atgcgtctcc atgatacgaa acgtaccgta tcgttaaggt gttggttgaa       60

cagcctttac accaacgggt cacacgggta ttatgctagc                            100


<210>  74
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  74
tccgaatgac atgcgtctcc atatacatac atgcttgttt gtttgtaaac acagccttta       60

caccaacggg tcacacgggt attatgctag c                                      91


<210>  75
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  75
tccgaatgac atgcgtctcc atatttaaaa ttcttgttta aaacagcctt tacaccaacg       60

ggtcacacgg gtattatgct agc                                               83


<210>  76
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  76
tccgaatgac atgcgtctcc cggaatgaac gttcattccg acagccttta caccaacggg       60

tcacacgggt attatgctag c                                                 81


<210>  77
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  77
000

<210>  78
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  78
000

<210>  79
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  79
000

<210>  80
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  80
000

<210>  81
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  81
000

<210>  82
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  82
000

<210>  83
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  83
000

<210>  84
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA array

<400>  84
000

<210>  85
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA array

<400>  85
000

<210>  86
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA array

<400>  86
000

<210>  87
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA array

<400>  87
000

<210>  88
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA array

<400>  88
000

<210>  89
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA array

<400>  89
000

<210>  90
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA array

<400>  90
000

<210>  91
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA array

<400>  91
000

<210>  92
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA array

<400>  92
000

<210>  93
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  insulator

<400>  93
000

<210>  94
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene

<400>  94
000

<210>  95
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene

<400>  95
000

<210>  96
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene

<400>  96
000

<210>  97
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene

<400>  97
000

<210>  98
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene

<400>  98
000

<210>  99
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene

<400>  99
000

<210>  100
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene

<400>  100
000

<210>  101
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene

<400>  101
000

<210>  102
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene

<400>  102
000

<210>  103
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene

<400>  103
000

<210>  104
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gene

<400>  104
000

<210>  105
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  105
000

<210>  106
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  106
000

<210>  107
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  107
000

<210>  108
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  108
000

<210>  109
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  109
000

<210>  110
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  110
000

<210>  111
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  111
000

<210>  112
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  112
000

<210>  113
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  113
000

<210>  114
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  114
000

<210>  115
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  115
000

<210>  116
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  116
000

<210>  117
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  117
000

<210>  118
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  118
000

<210>  119
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  119
000

<210>  120
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  120
000

<210>  121
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  121
000

<210>  122
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  122
000

<210>  123
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  123
000

<210>  124
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  124
000

<210>  125
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  125
000

<210>  126
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  126
000

<210>  127
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Linker Sequence

<400>  127

Gly Gly Ser Gly Gly Ser 
1               5       


<210>  128
<211>  1368
<212>  PRT
<213>  Streptococcus pyogenes

<400>  128

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  129
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  129

Gly Gly Gly Ser 
1               


<210>  130
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  130

Gly Gly Gly Gly Gly Gly 
1               5       


<210>  131
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  131

Gly Gly Gly Gly Gly Gly Gly Gly 
1               5               


<210>  132
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  132

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15  


<210>  133
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  133

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys Ala Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala 
            20                  25                  30          


Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
        35                  40                  45      


<210>  134
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  134

Pro Ala Pro Ala Pro 
1               5   


<210>  135
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  135

Val Ser Gln Thr Ser Lys Leu Thr Arg Ala Glu Thr Val Phe Pro Asp 
1               5                   10                  15      


Val 
    

<210>  136
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  136

Arg Val Leu Ala Glu Ala 
1               5       


<210>  137
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  137

Glu Asp Val Val Cys Cys Ser Met Ser Tyr 
1               5                   10  


<210>  138
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  138

Gly Gly Ile Glu Gly Arg Gly Ser 
1               5               


<210>  139
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  139

Gly Phe Leu Gly 
1               
