                         SEQUENCE LISTING

<110>  Medin, Jeffrey A.
       Lum, Lawrence G.
       Oldham, Robyn A.A.
       Thakur, Archana
 
<120>  SYSTEM AND METHOD FOR THE DEVELOPMENT OF CD30 BISPECIFIC 
       ANTIBODIES FOR IMMUNOTHERAPY OF CD30+ MALIGNANCIES

<130>  650053.00641

<150>  US 62/735,576
<151>  2018-09-24

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - 8D10 light chain

<400>  1

Asp Ile Val Met Thr Gln Ser Pro Ala Ser Gln Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Ser Val Thr Ile Thr Cys Leu Ala Ser Gln Thr Ile Gly Thr Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ser Pro Gln Phe Leu Ile 
        35                  40                  45              


Tyr Ala Ala Thr Ser Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Lys Phe Ser Phe Lys Ile Ser Ser Leu Gln Ala 
65                  70                  75                  80  


Glu Asp Phe Val Ser Tyr Tyr Cys Gln Gln Leu Tyr Ser Thr Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  2
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL1

<400>  2

Leu Ala Ser Gln Thr Ile Gly Thr Trp Leu Ala 
1               5                   10      


<210>  3
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL2

<400>  3

Ala Ala Thr Ser Leu Ala Asp 
1               5           


<210>  4
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL3

<400>  4

Gln Gln Leu Tyr Ser Thr Pro Phe Thr 
1               5                   


<210>  5
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - 8D10 heavy chain

<400>  5

Gln Val Gln Leu Gln Glu Ser Gly Thr Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Met Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Asn Pro Ser Asn Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Asn Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Asp Tyr Tyr Tyr Gly Ser Ser Tyr Gly Phe Asp Val Trp 
            100                 105                 110         


Gly Thr Gly Thr Thr Val Thr Val Ser Ser 
        115                 120         


<210>  6
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH1

<400>  6

Gly Tyr Thr Phe Thr Ser Tyr 
1               5           


<210>  7
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH2

<400>  7

Asn Pro Ser Asn Gly Gly 
1               5       


<210>  8
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH3

<400>  8

Arg Asp Tyr Tyr Tyr Gly Ser Ser Tyr Gly Phe Asp Val 
1               5                   10              


<210>  9
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - 10C2 light chain

<400>  9

Asp Ile Val Leu Thr Gln Thr Pro Leu Thr Leu Ser Val Thr Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Asn Gln Ser Leu Leu Asp Ser 
            20                  25                  30          


Tyr Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  10
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL1

<400>  10

Lys Ser Asn Gln Ser Leu Leu Asp Ser Tyr Gly Lys Thr Tyr Leu Asn 
1               5                   10                  15      


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL2

<400>  11

Leu Val Ser Lys Leu Asp Ser 
1               5           


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL3

<400>  12

Trp Gln Gly Thr His Phe Pro Arg Thr 
1               5                   


<210>  13
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - 10C2 heavy chain

<400>  13

Gln Val Gln Leu Glu Gln Ser Gly Pro Val Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met Asn Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Val Ile Asn Pro Tyr Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Cys 
65                  70                  75                  80  


Met Glu Leu Asn Cys Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Leu Gly Ala Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
            100                 105                 110         


<210>  14
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH1

<400>  14

Gly Tyr Thr Phe Thr Asp Tyr 
1               5           


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH2

<400>  15

Asn Pro Tyr Asn Gly 
1               5   


<210>  16
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH3

<400>  16

Gly Ala Tyr 
1           


<210>  17
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - 12B1 light chain

<400>  17

Asp Ile Val Met Thr Gln Thr Thr Ala Ser Leu Ser Thr Ser Val Gly 
1               5                   10                  15      


Glu Thr Val Thr Ile Thr Cys Arg Ala Ser Gly Asn Leu His Ser Tyr 
            20                  25                  30          


Leu Thr Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val 
        35                  40                  45              


Tyr Asn Ala Lys Thr Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gln Tyr Ser Leu Lys Ile Asp Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Gly Ser Tyr Tyr Cys Gln His Phe Trp Thr Thr Pro Phe 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  18
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL1

<400>  18

Arg Ala Ser Gly Asn Leu His Ser Tyr Leu Thr 
1               5                   10      


<210>  19
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL2

<400>  19

Asn Ala Lys Thr Leu Ala Asp 
1               5           


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL3

<400>  20

Gln His Phe Trp Thr Thr Pro Phe Thr 
1               5                   


<210>  21
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - 12B1 heavy chain

<400>  21

Glu Val Lys Leu Glu Glu Ser Gly Thr Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Asn Pro Thr Asn Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Ser Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Arg Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Gly Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Asp Phe Ile Thr Thr Ser Gly Phe Ala Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ala 
        115                 120 


<210>  22
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH1

<400>  22

Gly Tyr Thr Phe Thr Ser Tyr 
1               5           


<210>  23
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH2

<400>  23

Asn Pro Thr Asn Gly Gly 
1               5       


<210>  24
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH3

<400>  24

Arg Asp Phe Ile Thr Thr Ser Gly Phe Ala Tyr 
1               5                   10      


<210>  25
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - 13H1 light chain

<400>  25

Asp Ile Val Met Thr Gln Thr Pro Lys Ser Met Ser Met Ser Val Gly 
1               5                   10                  15      


Glu Arg Val Thr Leu Ser Cys Lys Ala Ser Glu Asn Val Gly Thr Tyr 
            20                  25                  30          


Val Ser Trp Tyr Gln Gln Lys Pro Glu Gln Ser Pro Lys Val Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Asn Arg Phe Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Ala Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Thr 
65                  70                  75                  80  


Glu Asp Leu Ala Asp Tyr His Cys Gly Gln Ser Tyr Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105         


<210>  26
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL1

<400>  26

Lys Ala Ser Glu Asn Val Gly Thr Tyr Val Ser 
1               5                   10      


<210>  27
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL2

<400>  27

Gly Ala Ser Asn Arg Phe Thr 
1               5           


<210>  28
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL3

<400>  28

Gly Gln Ser Tyr Ser Tyr Pro Leu Thr 
1               5                   


<210>  29
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - 13H1 heavy chain

<400>  29

Gln Val Gln Leu Gln Gln Ser Gly Thr Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly His Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Asn Pro Ser Asn Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Ser Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Tyr Tyr Gly Ser Ser Ser Tyr Trp Ser Phe Asp Val 
            100                 105                 110         


Trp Gly Thr Gly Thr Thr Val Thr Val Ser Ser 
        115                 120             


<210>  30
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH1

<400>  30

Gly His Thr Phe Thr Ser Tyr 
1               5           


<210>  31
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH2

<400>  31

Asn Pro Ser Asn Gly Gly 
1               5       


<210>  32
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH3

<400>  32

Arg Gly Tyr Tyr Gly Ser Ser Ser Tyr Trp Ser Phe Asp Val 
1               5                   10                  


<210>  33
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - 15B8 light chain

<400>  33

Asp Ile Val Met Thr Gln Thr Pro Ala Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Glu Thr Val Thr Ile Thr Cys Arg Ala Ser Gly Asn Ile His Asn Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val 
        35                  40                  45              


Tyr Asn Ala Lys Thr Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gln Tyr Ser Leu Lys Ile Asn Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Gly Ser Tyr Tyr Cys Gln His Phe Trp Ser Thr Pro Phe 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  34
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL1

<400>  34

Arg Ala Ser Gly Asn Ile His Asn Tyr Leu Ala 
1               5                   10      


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL2

<400>  35

Asn Ala Lys Thr Leu Ala Asp 
1               5           


<210>  36
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDRL3

<400>  36

Gln His Phe Trp Ser Thr Pro Phe Thr 
1               5                   


<210>  37
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - 15B8 heavy chain

<400>  37

Gln Val Gln Leu Glu Gln Ser Gly Thr Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Asn Pro Ser Asn Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Ser Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Asn Asn Tyr Tyr Ala Ser Ser Pro Phe Ala Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Ser Val Ser Ala 
        115                 120     


<210>  38
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH1

<400>  38

Gly Tyr Thr Phe Thr Ser Tyr 
1               5           


<210>  39
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH2

<400>  39

Asn Pro Ser Asn Gly Gly 
1               5       


<210>  40
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic - CDH3

<400>  40

Arg Asn Asn Tyr Tyr Ala Ser Ser Pro Phe Ala Tyr 
1               5                   10          


