                         SEQUENCE LISTING

<110>  IPSEN BIOPHARM LIMITED
 
<120>  CLOSTRIDIAL NEUROTOXINS COMPRISING AN EXOGENOUS ACTIVATION LOOP

<130>  P59901WO

<150>  GB1815817.0
<151>  2018-09-28

<160>  81    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C1 Activation Loop Consensus Sequence


<220>
<221>  SITE
<222>  (2)..(2)
<223>  Xaa is 1-10 residues of any amino acid

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is Asp or Glu

<220>
<221>  SITE
<222>  (7)..(7)
<223>  Xaa is 4-15 residues of any amino acid

<400>  1

Cys Xaa Ile Xaa Gly Arg Xaa Cys 
1               5               


<210>  2
<211>  17
<212>  PRT
<213>  Clostridium botulinum

<400>  2

Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn Lys Thr Leu Asp 
1               5                   10                  15      


Cys 
    


<210>  3
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C1 Activation Loop Variant

<400>  3

Cys His Lys Ala Ile Glu Gly Arg Ser Leu Tyr Asn Lys Thr Leu Asp 
1               5                   10                  15      


Cys 
    


<210>  4
<211>  3891
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BoNT/X with a C1 Activation Loop

<400>  4
atgaaactgg aaatcaacaa attcaactac aacgatccga tcgatggcat taatgttatt       60

accatgcgtc cgcctcgtca tagcgataaa atcaataaag gtaaaggtcc gttcaaagcc      120

tttcaggtga ttaaaaacat ttggattgtg ccggaacgct acaactttac caataatacc      180

aacgatctga acattccgag cgaaccgatt atggaagcag atgccattta taacccgaac      240

tatctgaata ccccgagcga aaaagatgaa tttctgcagg gtgttatcaa agtgctggaa      300

cgcattaaaa gcaaaccgga aggtgaaaaa ctgctggaac tgattagcag cagcattccg      360

ctgccgctgg ttagcaatgg tgcactgacc ctgagcgata atgaaaccat tgcatatcaa      420

gagaacaaca acattgtgag caatctgcag gcaaacctgg ttatttatgg tccgggtcct      480

gatattgcaa ataatgcaac ctatggtctg tatagcaccc cgattagtaa tggtgaaggt      540

acactgagcg aagttagctt tagcccgttt tatctgaaac cgtttgatga aagctatggc      600

aattatcgta gcctggtgaa tatcgtgaac aaattcgtga aacgtgaatt tgcacctgat      660

ccggcaagca ccctgatgca tgaactggtt catgttaccc ataatctgta tggtattagc      720

aaccgcaact tctactataa ctttgacacc ggcaaaattg aaaccagccg tcagcagaat      780

agcctgattt ttgaagaact gctgaccttt ggtggcattg atagcaaagc aattagcagc      840

ctgatcatca agaaaattat cgaaaccgcc aagaacaact ataccacgct gattagcgaa      900

cgcctgaata ccgttaccgt tgaaaatgat ctgctgaaat atatcaaaaa caaaatcccg      960

gttcagggtc gtctgggtaa ctttaaactg gataccgcag aattcgagaa aaagctgaat     1020

accattctgt ttgtgctgaa cgaaagcaat ctggcacagc gttttagcat tctggttcgt     1080

aaacattacc tgaaagaacg tccgattgat ccgatttatg tgaacattct ggatgacaat     1140

agctacagca ccctggaagg ttttaacatt agcagtcagg gtagcaatga tttccaaggt     1200

cagctgctgg aaagcagcta ttttgaaaaa attgaaagca atgccctgcg tgcctttatc     1260

aaaatttgtc ataaagccat tgatggtcgc agcctgtata acaaaaccct ggattgtatt     1320

gaggtggaaa acaaagacct gtttctgatt agcaacaaag atagcctgaa cgatattaac     1380

ctgagcgaag aaaaaatcaa accggaaacc accgtgttct tcaaagataa actgcctccg     1440

caggatatta cgctgagcaa ttatgatttt accgaagcca atagcattcc gagcattagc     1500

cagcagaaca ttctggaacg taatgaagaa ctgtatgaac cgattcgcaa tagcctgttt     1560

gaaatcaaaa ccatctatgt ggataagctg accacctttc attttctgga agcccagaat     1620

attgatgaga gcattgatag cagcaaaatt cgtgttgaac tgaccgatag cgttgatgaa     1680

gcactgagca atccgaataa agtttatagc ccgttcaaga acatgagcaa caccattaat     1740

agcattgaaa ccggtattac cagcacctac atcttttatc agtggctgcg tagcatcgtg     1800

aaagatttta gtgatgaaac cggcaaaatc gacgtgattg ataaaagcag cgataccctg     1860

gcaattgttc cgtatattgg tccgctgctg aatattggta atgatattcg tcatggcgat     1920

tttgtgggtg caattgaact ggcaggcatt accgcactgc tggaatatgt tccggaattt     1980

accattccga ttctggttgg tctggaagtt attggtggcg aactggcacg tgaacaggtt     2040

gaagcaattg ttaataatgc cctggataaa cgcgatcaga aatgggcaga agtttacaat     2100

attaccaaag cacagtggtg gggcaccatt catttacaga ttaatacccg tctggcccat     2160

acctataaag ccctgagccg tcaggcaaat gccattaaaa tgaatatgga atttcagctg     2220

gccaactaca aaggcaacat tgatgataaa gccaagatca aaaacgccat cagcgaaacc     2280

gaaattctgc tgaacaaaag cgttgaacag gccatgaaaa acaccgagaa attcatgatt     2340

aaactgagca acagctacct gaccaaagaa atgattccga aagttcagga caacctgaaa     2400

aactttgatc tggaaaccaa aaagaccctg gacaagttca tcaaagagaa agaagatatc     2460

ctgggcacca atctgagcag cagcctgcgt cgtaaagtta gcattcgtct gaataaaaac     2520

attgccttcg acatcaacga tatcccgttt agcgaatttg atgatctgat caaccagtac     2580

aaaaacgaga tcgaagatta tgaagtgctg aatctgggtg cagaagatgg caaaatcaaa     2640

gatctgagcg gtacaaccag cgatatcaat attggttcag atatcgaact ggccgatggt     2700

cgtgaaaata aagcgattaa gattaaaggc agcgagaaca gcaccatcaa aattgcaatg     2760

aacaaatatc tgcgttttag cgcgaccgat aactttagca ttagcttttg gatcaaacat     2820

ccgaaaccga ccaatctgct taataacggt attgaatata ccctggtcga gaactttaat     2880

cagcgtggtt ggaaaattag catccaggat agcaaactga tttggtatct gcgcgatcac     2940

aataacagca tcaaaatcgt tacaccggat tatattgcgt ttaatggctg gaacctgatt     3000

accattacaa acaatcgtag caaaggcagc atcgtgtatg ttaacggtag caaaattgaa     3060

gagaaggaca ttagcagcat ttggaatacc gaagtggatg atccgattat cttccgcctg     3120

aaaaataacc gtgataccca ggcatttacc ctgctggatc agtttagcat ttatcgcaaa     3180

gaactgaacc agaacgaagt ggtgaaactg tataactact acttcaacag caactacatt     3240

cgcgatattt ggggtaatcc gctgcagtac aacaaaaaat actatctgca gacccaggac     3300

aaacctggta aaggtctgat ccgcgaatat tggagcagct ttggttatga ttatgtgatt     3360

ctgagcgata gcaagaccat tacctttccg aataatatcc gttatggtgc cctgtataat     3420

ggtagcaaag tgctgatcaa gaacagcaaa aaactggatg gtctggtgcg caataaagat     3480

ttcattcagc tggaaatcga tggctataat atgggtatta gcgcagatcg ctttaacgag     3540

gataccaact atattggcac cacctatggt acaacccatg atctgaccac cgattttgaa     3600

attattcagc gccaagagaa ataccgcaat tattgtcagc tgaaaacccc gtataacatc     3660

tttcataaaa gcggtctgat gagcaccgaa accagcaaac cgaccttcca tgattatcgc     3720

gattgggttt atagcagcgc atggtatttt cagaactatg aaaatctgaa cctgcgcaaa     3780

cataccaaaa ccaactggta ttttatcccg aaagatgaag gttgggatga agatctggaa     3840

gtgctgtttc agggtccgca tcatcaccac catcaccatc atcatcactg a              3891


<210>  5
<211>  1296
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BoNT/X with a C1 Activation Loop

<400>  5

Met Lys Leu Glu Ile Asn Lys Phe Asn Tyr Asn Asp Pro Ile Asp Gly 
1               5                   10                  15      


Ile Asn Val Ile Thr Met Arg Pro Pro Arg His Ser Asp Lys Ile Asn 
            20                  25                  30          


Lys Gly Lys Gly Pro Phe Lys Ala Phe Gln Val Ile Lys Asn Ile Trp 
        35                  40                  45              


Ile Val Pro Glu Arg Tyr Asn Phe Thr Asn Asn Thr Asn Asp Leu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Pro Ile Met Glu Ala Asp Ala Ile Tyr Asn Pro Asn 
65                  70                  75                  80  


Tyr Leu Asn Thr Pro Ser Glu Lys Asp Glu Phe Leu Gln Gly Val Ile 
                85                  90                  95      


Lys Val Leu Glu Arg Ile Lys Ser Lys Pro Glu Gly Glu Lys Leu Leu 
            100                 105                 110         


Glu Leu Ile Ser Ser Ser Ile Pro Leu Pro Leu Val Ser Asn Gly Ala 
        115                 120                 125             


Leu Thr Leu Ser Asp Asn Glu Thr Ile Ala Tyr Gln Glu Asn Asn Asn 
    130                 135                 140                 


Ile Val Ser Asn Leu Gln Ala Asn Leu Val Ile Tyr Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Ile Ala Asn Asn Ala Thr Tyr Gly Leu Tyr Ser Thr Pro Ile Ser 
                165                 170                 175     


Asn Gly Glu Gly Thr Leu Ser Glu Val Ser Phe Ser Pro Phe Tyr Leu 
            180                 185                 190         


Lys Pro Phe Asp Glu Ser Tyr Gly Asn Tyr Arg Ser Leu Val Asn Ile 
        195                 200                 205             


Val Asn Lys Phe Val Lys Arg Glu Phe Ala Pro Asp Pro Ala Ser Thr 
    210                 215                 220                 


Leu Met His Glu Leu Val His Val Thr His Asn Leu Tyr Gly Ile Ser 
225                 230                 235                 240 


Asn Arg Asn Phe Tyr Tyr Asn Phe Asp Thr Gly Lys Ile Glu Thr Ser 
                245                 250                 255     


Arg Gln Gln Asn Ser Leu Ile Phe Glu Glu Leu Leu Thr Phe Gly Gly 
            260                 265                 270         


Ile Asp Ser Lys Ala Ile Ser Ser Leu Ile Ile Lys Lys Ile Ile Glu 
        275                 280                 285             


Thr Ala Lys Asn Asn Tyr Thr Thr Leu Ile Ser Glu Arg Leu Asn Thr 
    290                 295                 300                 


Val Thr Val Glu Asn Asp Leu Leu Lys Tyr Ile Lys Asn Lys Ile Pro 
305                 310                 315                 320 


Val Gln Gly Arg Leu Gly Asn Phe Lys Leu Asp Thr Ala Glu Phe Glu 
                325                 330                 335     


Lys Lys Leu Asn Thr Ile Leu Phe Val Leu Asn Glu Ser Asn Leu Ala 
            340                 345                 350         


Gln Arg Phe Ser Ile Leu Val Arg Lys His Tyr Leu Lys Glu Arg Pro 
        355                 360                 365             


Ile Asp Pro Ile Tyr Val Asn Ile Leu Asp Asp Asn Ser Tyr Ser Thr 
    370                 375                 380                 


Leu Glu Gly Phe Asn Ile Ser Ser Gln Gly Ser Asn Asp Phe Gln Gly 
385                 390                 395                 400 


Gln Leu Leu Glu Ser Ser Tyr Phe Glu Lys Ile Glu Ser Asn Ala Leu 
                405                 410                 415     


Arg Ala Phe Ile Lys Ile Cys His Lys Ala Ile Asp Gly Arg Ser Leu 
            420                 425                 430         


Tyr Asn Lys Thr Leu Asp Cys Ile Glu Val Glu Asn Lys Asp Leu Phe 
        435                 440                 445             


Leu Ile Ser Asn Lys Asp Ser Leu Asn Asp Ile Asn Leu Ser Glu Glu 
    450                 455                 460                 


Lys Ile Lys Pro Glu Thr Thr Val Phe Phe Lys Asp Lys Leu Pro Pro 
465                 470                 475                 480 


Gln Asp Ile Thr Leu Ser Asn Tyr Asp Phe Thr Glu Ala Asn Ser Ile 
                485                 490                 495     


Pro Ser Ile Ser Gln Gln Asn Ile Leu Glu Arg Asn Glu Glu Leu Tyr 
            500                 505                 510         


Glu Pro Ile Arg Asn Ser Leu Phe Glu Ile Lys Thr Ile Tyr Val Asp 
        515                 520                 525             


Lys Leu Thr Thr Phe His Phe Leu Glu Ala Gln Asn Ile Asp Glu Ser 
    530                 535                 540                 


Ile Asp Ser Ser Lys Ile Arg Val Glu Leu Thr Asp Ser Val Asp Glu 
545                 550                 555                 560 


Ala Leu Ser Asn Pro Asn Lys Val Tyr Ser Pro Phe Lys Asn Met Ser 
                565                 570                 575     


Asn Thr Ile Asn Ser Ile Glu Thr Gly Ile Thr Ser Thr Tyr Ile Phe 
            580                 585                 590         


Tyr Gln Trp Leu Arg Ser Ile Val Lys Asp Phe Ser Asp Glu Thr Gly 
        595                 600                 605             


Lys Ile Asp Val Ile Asp Lys Ser Ser Asp Thr Leu Ala Ile Val Pro 
    610                 615                 620                 


Tyr Ile Gly Pro Leu Leu Asn Ile Gly Asn Asp Ile Arg His Gly Asp 
625                 630                 635                 640 


Phe Val Gly Ala Ile Glu Leu Ala Gly Ile Thr Ala Leu Leu Glu Tyr 
                645                 650                 655     


Val Pro Glu Phe Thr Ile Pro Ile Leu Val Gly Leu Glu Val Ile Gly 
            660                 665                 670         


Gly Glu Leu Ala Arg Glu Gln Val Glu Ala Ile Val Asn Asn Ala Leu 
        675                 680                 685             


Asp Lys Arg Asp Gln Lys Trp Ala Glu Val Tyr Asn Ile Thr Lys Ala 
    690                 695                 700                 


Gln Trp Trp Gly Thr Ile His Leu Gln Ile Asn Thr Arg Leu Ala His 
705                 710                 715                 720 


Thr Tyr Lys Ala Leu Ser Arg Gln Ala Asn Ala Ile Lys Met Asn Met 
                725                 730                 735     


Glu Phe Gln Leu Ala Asn Tyr Lys Gly Asn Ile Asp Asp Lys Ala Lys 
            740                 745                 750         


Ile Lys Asn Ala Ile Ser Glu Thr Glu Ile Leu Leu Asn Lys Ser Val 
        755                 760                 765             


Glu Gln Ala Met Lys Asn Thr Glu Lys Phe Met Ile Lys Leu Ser Asn 
    770                 775                 780                 


Ser Tyr Leu Thr Lys Glu Met Ile Pro Lys Val Gln Asp Asn Leu Lys 
785                 790                 795                 800 


Asn Phe Asp Leu Glu Thr Lys Lys Thr Leu Asp Lys Phe Ile Lys Glu 
                805                 810                 815     


Lys Glu Asp Ile Leu Gly Thr Asn Leu Ser Ser Ser Leu Arg Arg Lys 
            820                 825                 830         


Val Ser Ile Arg Leu Asn Lys Asn Ile Ala Phe Asp Ile Asn Asp Ile 
        835                 840                 845             


Pro Phe Ser Glu Phe Asp Asp Leu Ile Asn Gln Tyr Lys Asn Glu Ile 
    850                 855                 860                 


Glu Asp Tyr Glu Val Leu Asn Leu Gly Ala Glu Asp Gly Lys Ile Lys 
865                 870                 875                 880 


Asp Leu Ser Gly Thr Thr Ser Asp Ile Asn Ile Gly Ser Asp Ile Glu 
                885                 890                 895     


Leu Ala Asp Gly Arg Glu Asn Lys Ala Ile Lys Ile Lys Gly Ser Glu 
            900                 905                 910         


Asn Ser Thr Ile Lys Ile Ala Met Asn Lys Tyr Leu Arg Phe Ser Ala 
        915                 920                 925             


Thr Asp Asn Phe Ser Ile Ser Phe Trp Ile Lys His Pro Lys Pro Thr 
    930                 935                 940                 


Asn Leu Leu Asn Asn Gly Ile Glu Tyr Thr Leu Val Glu Asn Phe Asn 
945                 950                 955                 960 


Gln Arg Gly Trp Lys Ile Ser Ile Gln Asp Ser Lys Leu Ile Trp Tyr 
                965                 970                 975     


Leu Arg Asp His Asn Asn Ser Ile Lys Ile Val Thr Pro Asp Tyr Ile 
            980                 985                 990         


Ala Phe Asn Gly Trp Asn Leu Ile  Thr Ile Thr Asn Asn  Arg Ser Lys 
        995                 1000                 1005             


Gly Ser  Ile Val Tyr Val Asn  Gly Ser Lys Ile Glu  Glu Lys Asp 
    1010                 1015                 1020             


Ile Ser  Ser Ile Trp Asn Thr  Glu Val Asp Asp Pro  Ile Ile Phe 
    1025                 1030                 1035             


Arg Leu  Lys Asn Asn Arg Asp  Thr Gln Ala Phe Thr  Leu Leu Asp 
    1040                 1045                 1050             


Gln Phe  Ser Ile Tyr Arg Lys  Glu Leu Asn Gln Asn  Glu Val Val 
    1055                 1060                 1065             


Lys Leu  Tyr Asn Tyr Tyr Phe  Asn Ser Asn Tyr Ile  Arg Asp Ile 
    1070                 1075                 1080             


Trp Gly  Asn Pro Leu Gln Tyr  Asn Lys Lys Tyr Tyr  Leu Gln Thr 
    1085                 1090                 1095             


Gln Asp  Lys Pro Gly Lys Gly  Leu Ile Arg Glu Tyr  Trp Ser Ser 
    1100                 1105                 1110             


Phe Gly  Tyr Asp Tyr Val Ile  Leu Ser Asp Ser Lys  Thr Ile Thr 
    1115                 1120                 1125             


Phe Pro  Asn Asn Ile Arg Tyr  Gly Ala Leu Tyr Asn  Gly Ser Lys 
    1130                 1135                 1140             


Val Leu  Ile Lys Asn Ser Lys  Lys Leu Asp Gly Leu  Val Arg Asn 
    1145                 1150                 1155             


Lys Asp  Phe Ile Gln Leu Glu  Ile Asp Gly Tyr Asn  Met Gly Ile 
    1160                 1165                 1170             


Ser Ala  Asp Arg Phe Asn Glu  Asp Thr Asn Tyr Ile  Gly Thr Thr 
    1175                 1180                 1185             


Tyr Gly  Thr Thr His Asp Leu  Thr Thr Asp Phe Glu  Ile Ile Gln 
    1190                 1195                 1200             


Arg Gln  Glu Lys Tyr Arg Asn  Tyr Cys Gln Leu Lys  Thr Pro Tyr 
    1205                 1210                 1215             


Asn Ile  Phe His Lys Ser Gly  Leu Met Ser Thr Glu  Thr Ser Lys 
    1220                 1225                 1230             


Pro Thr  Phe His Asp Tyr Arg  Asp Trp Val Tyr Ser  Ser Ala Trp 
    1235                 1240                 1245             


Tyr Phe  Gln Asn Tyr Glu Asn  Leu Asn Leu Arg Lys  His Thr Lys 
    1250                 1255                 1260             


Thr Asn  Trp Tyr Phe Ile Pro  Lys Asp Glu Gly Trp  Asp Glu Asp 
    1265                 1270                 1275             


Leu Glu  Val Leu Phe Gln Gly  Pro His His His His  His His His 
    1280                 1285                 1290             


His His  His 
    1295     


<210>  6
<211>  3993
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BoNT/XA [LHNX-HCA] with a C1 Activation Loop

<400>  6
atgaaactgg aaatcaacaa attcaactac aacgatccga tcgatggcat taatgttatt       60

accatgcgtc cgcctcgtca tagcgataaa atcaataaag gtaaaggtcc gttcaaagcc      120

tttcaggtga ttaaaaacat ttggattgtg ccggaacgct acaactttac caataatacc      180

aacgatctga acattccgag cgaaccgatt atggaagcag atgccattta taacccgaac      240

tatctgaata ccccgagcga aaaagatgaa tttctgcagg gtgttatcaa agtgctggaa      300

cgcattaaaa gcaaaccgga aggtgaaaaa ctgctggaac tgattagcag cagcattccg      360

ctgccgctgg ttagcaatgg tgcactgacc ctgagcgata atgaaaccat tgcatatcaa      420

gagaacaaca acattgtgag caatctgcag gcaaacctgg ttatttatgg tccgggtcct      480

gatattgcaa ataatgcaac ctatggtctg tatagcaccc cgattagtaa tggtgaaggt      540

acactgagcg aagttagctt tagcccgttt tatctgaaac cgtttgatga aagctatggc      600

aattatcgta gcctggtgaa tatcgtgaac aaattcgtga aacgtgaatt tgcacctgat      660

ccggcaagca ccctgatgca tgaactggtt catgttaccc ataatctgta tggtattagc      720

aaccgcaact tctactataa ctttgacacc ggcaaaattg aaaccagccg tcagcagaat      780

agcctgattt ttgaagaact gctgaccttt ggtggcattg atagcaaagc aattagcagc      840

ctgatcatca agaaaattat cgaaaccgcc aagaacaact ataccacgct gattagcgaa      900

cgcctgaata ccgttaccgt tgaaaatgat ctgctgaaat atatcaaaaa caaaatcccg      960

gttcagggtc gtctgggtaa ctttaaactg gataccgcag aattcgagaa aaagctgaat     1020

accattctgt ttgtgctgaa cgaaagcaat ctggcacagc gttttagcat tctggttcgt     1080

aaacattacc tgaaagaacg tccgattgat ccgatttatg tgaacattct ggatgacaat     1140

agctacagca ccctggaagg ttttaacatt agcagtcagg gtagcaatga ttttcagggc     1200

cagctgctgg aaagcagcta ttttgaaaaa attgaatcca atgcgctgcg tgcctttatc     1260

aaaatttgtc ataaagccat tgatggtcgc agcctgtata acaaaaccct ggattgtatt     1320

gaagtggaaa acaaagacct gttcctgatt agcaataaag atagcctgaa cgatatcaac     1380

ctgagcgaag aaaaaatcaa accggaaacc accgtgttct tcaaagataa actgcctccg     1440

caggatatta ccctgagcaa ttatgatttt accgaagcca atagcattcc gagcattagc     1500

cagcagaaca ttctggaacg taatgaagaa ctgtatgaac cgattcgcaa tagcctgttt     1560

gaaatcaaaa ccatctatgt ggataagctg accacctttc attttctgga agcccagaat     1620

attgatgaga gcattgatag cagcaaaatt cgtgttgaac tgaccgatag cgttgatgaa     1680

gcactgagca atccgaataa agtttatagc ccgttcaaga acatgagcaa caccattaat     1740

agcattgaaa ccggtattac cagcacctac atcttttatc agtggctgcg tagcatcgtg     1800

aaagatttta gtgatgaaac cggcaaaatc gacgtgattg ataaaagcag cgataccctg     1860

gccattgttc cgtatattgg tccgctgctg aatattggta atgatattcg tcatggcgat     1920

tttgtgggtg caattgaact ggcaggcatt accgcactgc tggaatatgt tccggaattt     1980

accattccga ttctggttgg tctggaagtt attggtggcg aactggcacg tgaacaggtt     2040

gaagcaattg ttaataatgc cctggataaa cgcgatcaga aatgggcaga agtttacaat     2100

attaccaaag cacagtggtg gggcaccatt catttacaga ttaatacccg tctggcccat     2160

acctataaag ccctgagccg tcaggcaaat gccattaaaa tgaatatgga atttcagctg     2220

gccaactaca aaggcaacat tgatgataaa gccaagatca aaaacgccat cagcgaaacc     2280

gaaattctgc tgaacaaaag cgttgaacag gccatgaaaa acaccgagaa attcatgatt     2340

aaactgagca acagctacct gaccaaagaa atgattccga aagttcagga caacctgaaa     2400

aactttgatc tggaaaccaa aaagaccctg gacaagttca tcaaagagaa agaagatatc     2460

ctgggcacca atctgagcag cagcctgcgt cgtaaagtta gcattcgtct gaataaaaac     2520

attgccttcg acatcaacga tatcccgttt agcgaatttg atgatctgat caaccagtac     2580

aaaaacgaga tcgaagatta tgaagtgctg aatctgggtg cagaagatgg caaaatcaaa     2640

gatctgagcg gtacaaccag cgatattaac attggtagcg atatcgaaat catcaacacc     2700

agcattctga atctgcgcta tgaaagcaat catctgattg atctgagccg ttatgcgtcc     2760

aaaatcaata ttggcagcaa agtgaatttc gacccgatcg ataaaaatca gatccagctg     2820

tttaatctgg aaagctccaa aattgaggtg attctgaaaa acgcgattgt gtacaatagc     2880

atgtatgaga atttctcaac cagcttctgg attcgcattc cgaaatactt taacagcatc     2940

agcctgaaca acgagtatac cattatcaac tgcatggaaa acaatagcgg ttggaaagtg     3000

agcctgaatt atggtgaaat tatctggacc ctgcaggata cccaagaaat caaacagcgt     3060

gttgtgttca aatacagcca gatgattaac atcagcgatt acattaaccg ctggatcttt     3120

gttaccatta ccaacaatcg cctgaataac agcaagatct atattaacgg tcgtctgatt     3180

gaccagaaac cgattagtaa tctgggtaat attcatgcca gcaacaacat catgttcaaa     3240

ctggatggtt gtcgtgatac ccatcgttat atttggatca agtattttaa cctgtttgat     3300

aaagaactga acgaaaaaga aattaaggat ctgtatgata accagtccaa tagcggcatc     3360

ctgaaggatt tttggggtga ttatctgcag tatgacaaac cgtattatat gctgaacctg     3420

tacgatccga acaaatatgt ggatgtgaat aatgtgggta tccgtggcta tatgtatctg     3480

aaaggtccgc gtggtagcgt tatgaccacc aacatttatc tgaatagcag cctgtatcgt     3540

ggcaccaaat tcatcatcaa aaaatacgcc agcggcaaca aagataatat tgtgcgtaat     3600

aatgaccgcg tgtatatcaa tgtggtggtg aagaataaag aatatcgtct ggcaaccaat     3660

gcaagccagg caggcgttga aaaaattctg agcgcactgg aaatcccgga tgtgggtaat     3720

ctgagccagg ttgttgttat gaaaagcaaa aatgatcagg gcatcaccaa caagtgcaaa     3780

atgaatctgc aggacaataa cggcaacgac attggtttta ttggctttca ccagtttaac     3840

aacattgcca aactggttgc gagcaattgg tataatcgtc agattgaacg tagcagtcgt     3900

accctgggtt gtagctggga atttattccg gttgatgatg gttggggtga acgtccgctg     3960

catcatcacc accatcacca tcaccaccat taa                                  3993


<210>  7
<211>  1330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BoNT/XA [LHNX-HCA] with a C1 Activation Loop

<400>  7

Met Lys Leu Glu Ile Asn Lys Phe Asn Tyr Asn Asp Pro Ile Asp Gly 
1               5                   10                  15      


Ile Asn Val Ile Thr Met Arg Pro Pro Arg His Ser Asp Lys Ile Asn 
            20                  25                  30          


Lys Gly Lys Gly Pro Phe Lys Ala Phe Gln Val Ile Lys Asn Ile Trp 
        35                  40                  45              


Ile Val Pro Glu Arg Tyr Asn Phe Thr Asn Asn Thr Asn Asp Leu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Pro Ile Met Glu Ala Asp Ala Ile Tyr Asn Pro Asn 
65                  70                  75                  80  


Tyr Leu Asn Thr Pro Ser Glu Lys Asp Glu Phe Leu Gln Gly Val Ile 
                85                  90                  95      


Lys Val Leu Glu Arg Ile Lys Ser Lys Pro Glu Gly Glu Lys Leu Leu 
            100                 105                 110         


Glu Leu Ile Ser Ser Ser Ile Pro Leu Pro Leu Val Ser Asn Gly Ala 
        115                 120                 125             


Leu Thr Leu Ser Asp Asn Glu Thr Ile Ala Tyr Gln Glu Asn Asn Asn 
    130                 135                 140                 


Ile Val Ser Asn Leu Gln Ala Asn Leu Val Ile Tyr Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Ile Ala Asn Asn Ala Thr Tyr Gly Leu Tyr Ser Thr Pro Ile Ser 
                165                 170                 175     


Asn Gly Glu Gly Thr Leu Ser Glu Val Ser Phe Ser Pro Phe Tyr Leu 
            180                 185                 190         


Lys Pro Phe Asp Glu Ser Tyr Gly Asn Tyr Arg Ser Leu Val Asn Ile 
        195                 200                 205             


Val Asn Lys Phe Val Lys Arg Glu Phe Ala Pro Asp Pro Ala Ser Thr 
    210                 215                 220                 


Leu Met His Glu Leu Val His Val Thr His Asn Leu Tyr Gly Ile Ser 
225                 230                 235                 240 


Asn Arg Asn Phe Tyr Tyr Asn Phe Asp Thr Gly Lys Ile Glu Thr Ser 
                245                 250                 255     


Arg Gln Gln Asn Ser Leu Ile Phe Glu Glu Leu Leu Thr Phe Gly Gly 
            260                 265                 270         


Ile Asp Ser Lys Ala Ile Ser Ser Leu Ile Ile Lys Lys Ile Ile Glu 
        275                 280                 285             


Thr Ala Lys Asn Asn Tyr Thr Thr Leu Ile Ser Glu Arg Leu Asn Thr 
    290                 295                 300                 


Val Thr Val Glu Asn Asp Leu Leu Lys Tyr Ile Lys Asn Lys Ile Pro 
305                 310                 315                 320 


Val Gln Gly Arg Leu Gly Asn Phe Lys Leu Asp Thr Ala Glu Phe Glu 
                325                 330                 335     


Lys Lys Leu Asn Thr Ile Leu Phe Val Leu Asn Glu Ser Asn Leu Ala 
            340                 345                 350         


Gln Arg Phe Ser Ile Leu Val Arg Lys His Tyr Leu Lys Glu Arg Pro 
        355                 360                 365             


Ile Asp Pro Ile Tyr Val Asn Ile Leu Asp Asp Asn Ser Tyr Ser Thr 
    370                 375                 380                 


Leu Glu Gly Phe Asn Ile Ser Ser Gln Gly Ser Asn Asp Phe Gln Gly 
385                 390                 395                 400 


Gln Leu Leu Glu Ser Ser Tyr Phe Glu Lys Ile Glu Ser Asn Ala Leu 
                405                 410                 415     


Arg Ala Phe Ile Lys Ile Cys His Lys Ala Ile Asp Gly Arg Ser Leu 
            420                 425                 430         


Tyr Asn Lys Thr Leu Asp Cys Ile Glu Val Glu Asn Lys Asp Leu Phe 
        435                 440                 445             


Leu Ile Ser Asn Lys Asp Ser Leu Asn Asp Ile Asn Leu Ser Glu Glu 
    450                 455                 460                 


Lys Ile Lys Pro Glu Thr Thr Val Phe Phe Lys Asp Lys Leu Pro Pro 
465                 470                 475                 480 


Gln Asp Ile Thr Leu Ser Asn Tyr Asp Phe Thr Glu Ala Asn Ser Ile 
                485                 490                 495     


Pro Ser Ile Ser Gln Gln Asn Ile Leu Glu Arg Asn Glu Glu Leu Tyr 
            500                 505                 510         


Glu Pro Ile Arg Asn Ser Leu Phe Glu Ile Lys Thr Ile Tyr Val Asp 
        515                 520                 525             


Lys Leu Thr Thr Phe His Phe Leu Glu Ala Gln Asn Ile Asp Glu Ser 
    530                 535                 540                 


Ile Asp Ser Ser Lys Ile Arg Val Glu Leu Thr Asp Ser Val Asp Glu 
545                 550                 555                 560 


Ala Leu Ser Asn Pro Asn Lys Val Tyr Ser Pro Phe Lys Asn Met Ser 
                565                 570                 575     


Asn Thr Ile Asn Ser Ile Glu Thr Gly Ile Thr Ser Thr Tyr Ile Phe 
            580                 585                 590         


Tyr Gln Trp Leu Arg Ser Ile Val Lys Asp Phe Ser Asp Glu Thr Gly 
        595                 600                 605             


Lys Ile Asp Val Ile Asp Lys Ser Ser Asp Thr Leu Ala Ile Val Pro 
    610                 615                 620                 


Tyr Ile Gly Pro Leu Leu Asn Ile Gly Asn Asp Ile Arg His Gly Asp 
625                 630                 635                 640 


Phe Val Gly Ala Ile Glu Leu Ala Gly Ile Thr Ala Leu Leu Glu Tyr 
                645                 650                 655     


Val Pro Glu Phe Thr Ile Pro Ile Leu Val Gly Leu Glu Val Ile Gly 
            660                 665                 670         


Gly Glu Leu Ala Arg Glu Gln Val Glu Ala Ile Val Asn Asn Ala Leu 
        675                 680                 685             


Asp Lys Arg Asp Gln Lys Trp Ala Glu Val Tyr Asn Ile Thr Lys Ala 
    690                 695                 700                 


Gln Trp Trp Gly Thr Ile His Leu Gln Ile Asn Thr Arg Leu Ala His 
705                 710                 715                 720 


Thr Tyr Lys Ala Leu Ser Arg Gln Ala Asn Ala Ile Lys Met Asn Met 
                725                 730                 735     


Glu Phe Gln Leu Ala Asn Tyr Lys Gly Asn Ile Asp Asp Lys Ala Lys 
            740                 745                 750         


Ile Lys Asn Ala Ile Ser Glu Thr Glu Ile Leu Leu Asn Lys Ser Val 
        755                 760                 765             


Glu Gln Ala Met Lys Asn Thr Glu Lys Phe Met Ile Lys Leu Ser Asn 
    770                 775                 780                 


Ser Tyr Leu Thr Lys Glu Met Ile Pro Lys Val Gln Asp Asn Leu Lys 
785                 790                 795                 800 


Asn Phe Asp Leu Glu Thr Lys Lys Thr Leu Asp Lys Phe Ile Lys Glu 
                805                 810                 815     


Lys Glu Asp Ile Leu Gly Thr Asn Leu Ser Ser Ser Leu Arg Arg Lys 
            820                 825                 830         


Val Ser Ile Arg Leu Asn Lys Asn Ile Ala Phe Asp Ile Asn Asp Ile 
        835                 840                 845             


Pro Phe Ser Glu Phe Asp Asp Leu Ile Asn Gln Tyr Lys Asn Glu Ile 
    850                 855                 860                 


Glu Asp Tyr Glu Val Leu Asn Leu Gly Ala Glu Asp Gly Lys Ile Lys 
865                 870                 875                 880 


Asp Leu Ser Gly Thr Thr Ser Asp Ile Asn Ile Gly Ser Asp Ile Glu 
                885                 890                 895     


Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu 
            900                 905                 910         


Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val 
        915                 920                 925             


Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu 
    930                 935                 940                 


Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser 
945                 950                 955                 960 


Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr 
                965                 970                 975     


Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met 
            980                 985                 990         


Glu Asn Asn Ser Gly Trp Lys Val  Ser Leu Asn Tyr Gly  Glu Ile Ile 
        995                 1000                 1005             


Trp Thr  Leu Gln Asp Thr Gln  Glu Ile Lys Gln Arg  Val Val Phe 
    1010                 1015                 1020             


Lys Tyr  Ser Gln Met Ile Asn  Ile Ser Asp Tyr Ile  Asn Arg Trp 
    1025                 1030                 1035             


Ile Phe  Val Thr Ile Thr Asn  Asn Arg Leu Asn Asn  Ser Lys Ile 
    1040                 1045                 1050             


Tyr Ile  Asn Gly Arg Leu Ile  Asp Gln Lys Pro Ile  Ser Asn Leu 
    1055                 1060                 1065             


Gly Asn  Ile His Ala Ser Asn  Asn Ile Met Phe Lys  Leu Asp Gly 
    1070                 1075                 1080             


Cys Arg  Asp Thr His Arg Tyr  Ile Trp Ile Lys Tyr  Phe Asn Leu 
    1085                 1090                 1095             


Phe Asp  Lys Glu Leu Asn Glu  Lys Glu Ile Lys Asp  Leu Tyr Asp 
    1100                 1105                 1110             


Asn Gln  Ser Asn Ser Gly Ile  Leu Lys Asp Phe Trp  Gly Asp Tyr 
    1115                 1120                 1125             


Leu Gln  Tyr Asp Lys Pro Tyr  Tyr Met Leu Asn Leu  Tyr Asp Pro 
    1130                 1135                 1140             


Asn Lys  Tyr Val Asp Val Asn  Asn Val Gly Ile Arg  Gly Tyr Met 
    1145                 1150                 1155             


Tyr Leu  Lys Gly Pro Arg Gly  Ser Val Met Thr Thr  Asn Ile Tyr 
    1160                 1165                 1170             


Leu Asn  Ser Ser Leu Tyr Arg  Gly Thr Lys Phe Ile  Ile Lys Lys 
    1175                 1180                 1185             


Tyr Ala  Ser Gly Asn Lys Asp  Asn Ile Val Arg Asn  Asn Asp Arg 
    1190                 1195                 1200             


Val Tyr  Ile Asn Val Val Val  Lys Asn Lys Glu Tyr  Arg Leu Ala 
    1205                 1210                 1215             


Thr Asn  Ala Ser Gln Ala Gly  Val Glu Lys Ile Leu  Ser Ala Leu 
    1220                 1225                 1230             


Glu Ile  Pro Asp Val Gly Asn  Leu Ser Gln Val Val  Val Met Lys 
    1235                 1240                 1245             


Ser Lys  Asn Asp Gln Gly Ile  Thr Asn Lys Cys Lys  Met Asn Leu 
    1250                 1255                 1260             


Gln Asp  Asn Asn Gly Asn Asp  Ile Gly Phe Ile Gly  Phe His Gln 
    1265                 1270                 1275             


Phe Asn  Asn Ile Ala Lys Leu  Val Ala Ser Asn Trp  Tyr Asn Arg 
    1280                 1285                 1290             


Gln Ile  Glu Arg Ser Ser Arg  Thr Leu Gly Cys Ser  Trp Glu Phe 
    1295                 1300                 1305             


Ile Pro  Val Asp Asp Gly Trp  Gly Glu Arg Pro Leu  His His His 
    1310                 1315                 1320             


His His  His His His His His  
    1325                 1330 


<210>  8
<211>  4017
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BoNT/XB [LHNX-HCB] with a C1 Activation Loop

<400>  8
atgaaactgg aaatcaacaa attcaactac aacgatccga tcgatggcat taatgttatt       60

accatgcgtc cgcctcgtca tagcgataaa atcaataaag gtaaaggtcc gttcaaagcc      120

tttcaggtga ttaaaaacat ttggattgtg ccggaacgct acaactttac caataatacc      180

aacgatctga acattccgag cgaaccgatt atggaagcag atgccattta taacccgaac      240

tatctgaata ccccgagcga aaaagatgaa tttctgcagg gtgttatcaa agtgctggaa      300

cgcattaaaa gcaaaccgga aggtgaaaaa ctgctggaac tgattagcag cagcattccg      360

ctgccgctgg ttagcaatgg tgcactgacc ctgagcgata atgaaaccat tgcatatcaa      420

gagaacaaca acattgtgag caatctgcag gcaaacctgg ttatttatgg tccgggtcct      480

gatattgcaa ataatgcaac ctatggtctg tatagcaccc cgattagtaa tggtgaaggt      540

acactgagcg aagttagctt tagcccgttt tatctgaaac cgtttgatga aagctatggc      600

aattatcgta gcctggtgaa tatcgtgaac aaattcgtga aacgtgaatt tgcacctgat      660

ccggcaagca ccctgatgca tgaactggtt catgttaccc ataatctgta tggtattagc      720

aaccgcaact tctactataa ctttgacacc ggcaaaattg aaaccagccg tcagcagaat      780

agcctgattt ttgaagaact gctgaccttt ggtggcattg atagcaaagc aattagcagc      840

ctgatcatca agaaaattat cgaaaccgcc aagaacaact ataccacgct gattagcgaa      900

cgcctgaata ccgttaccgt tgaaaatgat ctgctgaaat atatcaaaaa caaaatcccg      960

gttcagggtc gtctgggtaa ctttaaactg gataccgcag aattcgagaa aaagctgaat     1020

accattctgt ttgtgctgaa cgaaagcaat ctggcacagc gttttagcat tctggttcgt     1080

aaacattacc tgaaagaacg tccgattgat ccgatttatg tgaacattct ggatgacaat     1140

agctacagca ccctggaagg ttttaacatt agcagtcagg gtagcaatga ttttcagggc     1200

cagctgctgg aaagcagcta ttttgaaaaa attgaatcca atgcgctgcg tgcctttatc     1260

aaaatttgtc ataaagccat tgatggtcgc agcctgtata acaaaaccct ggattgtatt     1320

gaagtggaaa acaaagacct gttcctgatt agcaataaag atagcctgaa cgatatcaac     1380

ctgagcgaag aaaaaatcaa accggaaacc accgtgttct tcaaagataa actgcctccg     1440

caggatatta ccctgagcaa ttatgatttt accgaagcca atagcattcc gagcattagc     1500

cagcagaaca ttctggaacg taatgaagaa ctgtatgaac cgattcgcaa tagcctgttt     1560

gaaatcaaaa ccatctatgt ggataagctg accacctttc attttctgga agcccagaat     1620

attgatgaga gcattgatag cagcaaaatt cgtgttgaac tgaccgatag cgttgatgaa     1680

gcactgagca atccgaataa agtttatagc ccgttcaaga acatgagcaa caccattaat     1740

agcattgaaa ccggtattac cagcacctac atcttttatc agtggctgcg tagcatcgtg     1800

aaagatttta gtgatgaaac cggcaaaatc gacgtgattg ataaaagcag cgataccctg     1860

gccattgttc cgtatattgg tccgctgctg aatattggta atgatattcg tcatggcgat     1920

tttgtgggtg caattgaact ggcaggcatt accgcactgc tggaatatgt tccggaattt     1980

accattccga ttctggttgg tctggaagtt attggtggcg aactggcacg tgaacaggtt     2040

gaagcaattg ttaataatgc cctggataaa cgcgatcaga aatgggcaga agtttacaat     2100

attaccaaag cacagtggtg gggcaccatt catttacaga ttaatacccg tctggcccat     2160

acctataaag ccctgagccg tcaggcaaat gccattaaaa tgaatatgga atttcagctg     2220

gccaactaca aaggcaacat tgatgataaa gccaagatca aaaacgccat cagcgaaacc     2280

gaaattctgc tgaacaaaag cgttgaacag gccatgaaaa acaccgagaa attcatgatt     2340

aaactgagca acagctacct gaccaaagaa atgattccga aagttcagga caacctgaaa     2400

aactttgatc tggaaaccaa aaagaccctg gacaagttca tcaaagagaa agaagatatc     2460

ctgggcacca atctgagcag cagcctgcgt cgtaaagtta gcattcgtct gaataaaaac     2520

attgccttcg acatcaacga tatcccgttt agcgaatttg atgatctgat caaccagtac     2580

aaaaacgaga tcgaagatta tgaagtgctg aatctgggtg cagaagatgg caaaatcaaa     2640

gatctgagcg gtacaaccag cgatattaac attggtagcg atatcgaaat cctgaacaac     2700

attattctga acctgcgcta taaagataac aacctgattg atctgagtgg ctatggtgca     2760

aaagttgaag tttatgatgg tgtggaactg aacgacaaaa accagttcaa actgaccagc     2820

agcgcaaatt caaaaattcg cgttacccag aaccagaaca tcatttttaa cagcgtgttt     2880

ctggatttca gcgtgagctt ttggattcgt attccgaaat ataagaacga cggcatccag     2940

aactatatcc acaatgaata taccatcatc aactgcatga agaataacag cggttggaaa     3000

attagcatcc gtggcaatcg tattatttgg accctgatcg atattaatgg caaaaccaag     3060

agcgtgtttt tcgagtataa catccgtgaa gatatcagcg aatacatcaa ccgttggttt     3120

tttgtgacca ttaccaacaa tctgaacaac gccaaaatct acattaacgg caaactggaa     3180

agcaacaccg atatcaaaga tattcgtgaa gtgattgcca acggcgagat tatctttaaa     3240

ctggatggtg atattgatcg cacccagttt atttggatga aatacttcag catcttcaac     3300

accgaactga gccagagcaa tattgaagaa cgctataaaa tccagagcta cagcgagtat     3360

ctgaaagact tttggggtaa tccgctgatg tacaacaaag aatactacat gtttaatgcc     3420

ggtaacaaaa acagctatat caaactgaaa aaggatagtc cggtgggtga aattctgacc     3480

cgtagcaaat ataaccagaa tagcaagtat atcaactatc gcgatctgta catcggcgag     3540

aaatttatca ttcgtcgtaa aagcaactcc cagagcatta acgatgatat tgtgcgcaaa     3600

gaggattaca tctacctgga ttttttcaac ctgaatcaag agtggcgtgt gtacacctat     3660

aagtacttca aaaaagaaga aatgaaactg tttctggcac cgatctatga tagcgacgaa     3720

ttttacaata ccattcagat taaagaatat gatgaacagc cgacctatag ctgtcagctg     3780

ctgtttaaaa aggatgaaga aagcacggat gaaattggcc tgattggtat ccatcgtttt     3840

tatgaaagcg gcatcgtgtt cgaagagtac aaagattatt tctgcatcag caaatggtat     3900

cttaaagagg tgaaacgcaa accgtataat ctgaaactgg gttgcaattg gcagttcatc     3960

ccgaaagatg aaggttggac cgaacatcat caccaccatc accatcatca tcactga        4017


<210>  9
<211>  1338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BoNT/XB [LHNX-HCB] with a C1 Activation Loop

<400>  9

Met Lys Leu Glu Ile Asn Lys Phe Asn Tyr Asn Asp Pro Ile Asp Gly 
1               5                   10                  15      


Ile Asn Val Ile Thr Met Arg Pro Pro Arg His Ser Asp Lys Ile Asn 
            20                  25                  30          


Lys Gly Lys Gly Pro Phe Lys Ala Phe Gln Val Ile Lys Asn Ile Trp 
        35                  40                  45              


Ile Val Pro Glu Arg Tyr Asn Phe Thr Asn Asn Thr Asn Asp Leu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Pro Ile Met Glu Ala Asp Ala Ile Tyr Asn Pro Asn 
65                  70                  75                  80  


Tyr Leu Asn Thr Pro Ser Glu Lys Asp Glu Phe Leu Gln Gly Val Ile 
                85                  90                  95      


Lys Val Leu Glu Arg Ile Lys Ser Lys Pro Glu Gly Glu Lys Leu Leu 
            100                 105                 110         


Glu Leu Ile Ser Ser Ser Ile Pro Leu Pro Leu Val Ser Asn Gly Ala 
        115                 120                 125             


Leu Thr Leu Ser Asp Asn Glu Thr Ile Ala Tyr Gln Glu Asn Asn Asn 
    130                 135                 140                 


Ile Val Ser Asn Leu Gln Ala Asn Leu Val Ile Tyr Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Ile Ala Asn Asn Ala Thr Tyr Gly Leu Tyr Ser Thr Pro Ile Ser 
                165                 170                 175     


Asn Gly Glu Gly Thr Leu Ser Glu Val Ser Phe Ser Pro Phe Tyr Leu 
            180                 185                 190         


Lys Pro Phe Asp Glu Ser Tyr Gly Asn Tyr Arg Ser Leu Val Asn Ile 
        195                 200                 205             


Val Asn Lys Phe Val Lys Arg Glu Phe Ala Pro Asp Pro Ala Ser Thr 
    210                 215                 220                 


Leu Met His Glu Leu Val His Val Thr His Asn Leu Tyr Gly Ile Ser 
225                 230                 235                 240 


Asn Arg Asn Phe Tyr Tyr Asn Phe Asp Thr Gly Lys Ile Glu Thr Ser 
                245                 250                 255     


Arg Gln Gln Asn Ser Leu Ile Phe Glu Glu Leu Leu Thr Phe Gly Gly 
            260                 265                 270         


Ile Asp Ser Lys Ala Ile Ser Ser Leu Ile Ile Lys Lys Ile Ile Glu 
        275                 280                 285             


Thr Ala Lys Asn Asn Tyr Thr Thr Leu Ile Ser Glu Arg Leu Asn Thr 
    290                 295                 300                 


Val Thr Val Glu Asn Asp Leu Leu Lys Tyr Ile Lys Asn Lys Ile Pro 
305                 310                 315                 320 


Val Gln Gly Arg Leu Gly Asn Phe Lys Leu Asp Thr Ala Glu Phe Glu 
                325                 330                 335     


Lys Lys Leu Asn Thr Ile Leu Phe Val Leu Asn Glu Ser Asn Leu Ala 
            340                 345                 350         


Gln Arg Phe Ser Ile Leu Val Arg Lys His Tyr Leu Lys Glu Arg Pro 
        355                 360                 365             


Ile Asp Pro Ile Tyr Val Asn Ile Leu Asp Asp Asn Ser Tyr Ser Thr 
    370                 375                 380                 


Leu Glu Gly Phe Asn Ile Ser Ser Gln Gly Ser Asn Asp Phe Gln Gly 
385                 390                 395                 400 


Gln Leu Leu Glu Ser Ser Tyr Phe Glu Lys Ile Glu Ser Asn Ala Leu 
                405                 410                 415     


Arg Ala Phe Ile Lys Ile Cys His Lys Ala Ile Asp Gly Arg Ser Leu 
            420                 425                 430         


Tyr Asn Lys Thr Leu Asp Cys Ile Glu Val Glu Asn Lys Asp Leu Phe 
        435                 440                 445             


Leu Ile Ser Asn Lys Asp Ser Leu Asn Asp Ile Asn Leu Ser Glu Glu 
    450                 455                 460                 


Lys Ile Lys Pro Glu Thr Thr Val Phe Phe Lys Asp Lys Leu Pro Pro 
465                 470                 475                 480 


Gln Asp Ile Thr Leu Ser Asn Tyr Asp Phe Thr Glu Ala Asn Ser Ile 
                485                 490                 495     


Pro Ser Ile Ser Gln Gln Asn Ile Leu Glu Arg Asn Glu Glu Leu Tyr 
            500                 505                 510         


Glu Pro Ile Arg Asn Ser Leu Phe Glu Ile Lys Thr Ile Tyr Val Asp 
        515                 520                 525             


Lys Leu Thr Thr Phe His Phe Leu Glu Ala Gln Asn Ile Asp Glu Ser 
    530                 535                 540                 


Ile Asp Ser Ser Lys Ile Arg Val Glu Leu Thr Asp Ser Val Asp Glu 
545                 550                 555                 560 


Ala Leu Ser Asn Pro Asn Lys Val Tyr Ser Pro Phe Lys Asn Met Ser 
                565                 570                 575     


Asn Thr Ile Asn Ser Ile Glu Thr Gly Ile Thr Ser Thr Tyr Ile Phe 
            580                 585                 590         


Tyr Gln Trp Leu Arg Ser Ile Val Lys Asp Phe Ser Asp Glu Thr Gly 
        595                 600                 605             


Lys Ile Asp Val Ile Asp Lys Ser Ser Asp Thr Leu Ala Ile Val Pro 
    610                 615                 620                 


Tyr Ile Gly Pro Leu Leu Asn Ile Gly Asn Asp Ile Arg His Gly Asp 
625                 630                 635                 640 


Phe Val Gly Ala Ile Glu Leu Ala Gly Ile Thr Ala Leu Leu Glu Tyr 
                645                 650                 655     


Val Pro Glu Phe Thr Ile Pro Ile Leu Val Gly Leu Glu Val Ile Gly 
            660                 665                 670         


Gly Glu Leu Ala Arg Glu Gln Val Glu Ala Ile Val Asn Asn Ala Leu 
        675                 680                 685             


Asp Lys Arg Asp Gln Lys Trp Ala Glu Val Tyr Asn Ile Thr Lys Ala 
    690                 695                 700                 


Gln Trp Trp Gly Thr Ile His Leu Gln Ile Asn Thr Arg Leu Ala His 
705                 710                 715                 720 


Thr Tyr Lys Ala Leu Ser Arg Gln Ala Asn Ala Ile Lys Met Asn Met 
                725                 730                 735     


Glu Phe Gln Leu Ala Asn Tyr Lys Gly Asn Ile Asp Asp Lys Ala Lys 
            740                 745                 750         


Ile Lys Asn Ala Ile Ser Glu Thr Glu Ile Leu Leu Asn Lys Ser Val 
        755                 760                 765             


Glu Gln Ala Met Lys Asn Thr Glu Lys Phe Met Ile Lys Leu Ser Asn 
    770                 775                 780                 


Ser Tyr Leu Thr Lys Glu Met Ile Pro Lys Val Gln Asp Asn Leu Lys 
785                 790                 795                 800 


Asn Phe Asp Leu Glu Thr Lys Lys Thr Leu Asp Lys Phe Ile Lys Glu 
                805                 810                 815     


Lys Glu Asp Ile Leu Gly Thr Asn Leu Ser Ser Ser Leu Arg Arg Lys 
            820                 825                 830         


Val Ser Ile Arg Leu Asn Lys Asn Ile Ala Phe Asp Ile Asn Asp Ile 
        835                 840                 845             


Pro Phe Ser Glu Phe Asp Asp Leu Ile Asn Gln Tyr Lys Asn Glu Ile 
    850                 855                 860                 


Glu Asp Tyr Glu Val Leu Asn Leu Gly Ala Glu Asp Gly Lys Ile Lys 
865                 870                 875                 880 


Asp Leu Ser Gly Thr Thr Ser Asp Ile Asn Ile Gly Ser Asp Ile Glu 
                885                 890                 895     


Ile Leu Asn Asn Ile Ile Leu Asn Leu Arg Tyr Lys Asp Asn Asn Leu 
            900                 905                 910         


Ile Asp Leu Ser Gly Tyr Gly Ala Lys Val Glu Val Tyr Asp Gly Val 
        915                 920                 925             


Glu Leu Asn Asp Lys Asn Gln Phe Lys Leu Thr Ser Ser Ala Asn Ser 
    930                 935                 940                 


Lys Ile Arg Val Thr Gln Asn Gln Asn Ile Ile Phe Asn Ser Val Phe 
945                 950                 955                 960 


Leu Asp Phe Ser Val Ser Phe Trp Ile Arg Ile Pro Lys Tyr Lys Asn 
                965                 970                 975     


Asp Gly Ile Gln Asn Tyr Ile His Asn Glu Tyr Thr Ile Ile Asn Cys 
            980                 985                 990         


Met Lys Asn Asn Ser Gly Trp Lys  Ile Ser Ile Arg Gly  Asn Arg Ile 
        995                 1000                 1005             


Ile Trp  Thr Leu Ile Asp Ile  Asn Gly Lys Thr Lys  Ser Val Phe 
    1010                 1015                 1020             


Phe Glu  Tyr Asn Ile Arg Glu  Asp Ile Ser Glu Tyr  Ile Asn Arg 
    1025                 1030                 1035             


Trp Phe  Phe Val Thr Ile Thr  Asn Asn Leu Asn Asn  Ala Lys Ile 
    1040                 1045                 1050             


Tyr Ile  Asn Gly Lys Leu Glu  Ser Asn Thr Asp Ile  Lys Asp Ile 
    1055                 1060                 1065             


Arg Glu  Val Ile Ala Asn Gly  Glu Ile Ile Phe Lys  Leu Asp Gly 
    1070                 1075                 1080             


Asp Ile  Asp Arg Thr Gln Phe  Ile Trp Met Lys Tyr  Phe Ser Ile 
    1085                 1090                 1095             


Phe Asn  Thr Glu Leu Ser Gln  Ser Asn Ile Glu Glu  Arg Tyr Lys 
    1100                 1105                 1110             


Ile Gln  Ser Tyr Ser Glu Tyr  Leu Lys Asp Phe Trp  Gly Asn Pro 
    1115                 1120                 1125             


Leu Met  Tyr Asn Lys Glu Tyr  Tyr Met Phe Asn Ala  Gly Asn Lys 
    1130                 1135                 1140             


Asn Ser  Tyr Ile Lys Leu Lys  Lys Asp Ser Pro Val  Gly Glu Ile 
    1145                 1150                 1155             


Leu Thr  Arg Ser Lys Tyr Asn  Gln Asn Ser Lys Tyr  Ile Asn Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Ile Gly Glu  Lys Phe Ile Ile Arg  Arg Lys Ser 
    1175                 1180                 1185             


Asn Ser  Gln Ser Ile Asn Asp  Asp Ile Val Arg Lys  Glu Asp Tyr 
    1190                 1195                 1200             


Ile Tyr  Leu Asp Phe Phe Asn  Leu Asn Gln Glu Trp  Arg Val Tyr 
    1205                 1210                 1215             


Thr Tyr  Lys Tyr Phe Lys Lys  Glu Glu Met Lys Leu  Phe Leu Ala 
    1220                 1225                 1230             


Pro Ile  Tyr Asp Ser Asp Glu  Phe Tyr Asn Thr Ile  Gln Ile Lys 
    1235                 1240                 1245             


Glu Tyr  Asp Glu Gln Pro Thr  Tyr Ser Cys Gln Leu  Leu Phe Lys 
    1250                 1255                 1260             


Lys Asp  Glu Glu Ser Thr Asp  Glu Ile Gly Leu Ile  Gly Ile His 
    1265                 1270                 1275             


Arg Phe  Tyr Glu Ser Gly Ile  Val Phe Glu Glu Tyr  Lys Asp Tyr 
    1280                 1285                 1290             


Phe Cys  Ile Ser Lys Trp Tyr  Leu Lys Glu Val Lys  Arg Lys Pro 
    1295                 1300                 1305             


Tyr Asn  Leu Lys Leu Gly Cys  Asn Trp Gln Phe Ile  Pro Lys Asp 
    1310                 1315                 1320             


Glu Gly  Trp Thr Glu His His  His His His His His  His His His 
    1325                 1330                 1335             


<210>  10
<211>  3762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BoNT/E with a C1 Activation Loop

<400>  10
atgccgaaaa tcaactcttt caactacaac gacccggtta acgaccgtac catcctgtat       60

atcaaaccgg gtggttgcca ggagttctac aaatctttca acatcatgaa aaacatctgg      120

atcatcccgg aacgtaacgt tatcggtacc accccgcagg acttccaccc gccgacctct      180

ctgaaaaacg gtgactcttc ttactacgac ccgaactacc tccagtctga cgaagaaaaa      240

gaccgtttcc tgaaaatcgt taccaaaatc ttcaaccgta tcaacaacaa cctgtctggt      300

ggtatcctgc tggaagaact gtctaaagct aacccgtacc tgggtaacga caacaccccg      360

gacaaccagt tccacatcgg tgacgcttct gctgttgaaa tcaaattctc taacggttct      420

caggacatcc tgctgccgaa cgttatcatc atgggtgctg aaccggacct gttcgaaacc      480

aactcttcta acatctctct gcgtaacaac tacatgccgt ctaaccacgg tttcggttct      540

atcgctatcg ttaccttctc tccggaatac tctttccgtt tcaacgacaa cagcatgaac      600

gagttcatcc aggacccggc tctgaccctg atgcaccaac tgatctattc tctgcacggt      660

ctgtacggtg ctaaaggtat caccaccaaa tacaccatca cccagaaaca gaacccgctg      720

atcaccaaca tccgtggtac caacatcgaa gagttcctga ccttcggtgg taccgacctg      780

aacatcatca cctctgctca gtctaacgac atctacacca acctgctggc tgactacaaa      840

aaaatcgctt ctaaactgtc taaagttcag gtttctaacc cgctgctgaa cccgtacaaa      900

gacgttttcg aagctaaata cggtctggac aaagacgctt ctggtatcta ctctgttaac      960

atcaacaaat tcaacgacat cttcaaaaaa ctgtactctt tcaccgagtt cgacctggcg     1020

accaaattcc aggttaaatg ccgtcagacc tacatcggtc agtacaaata cttcaaactg     1080

tctaacctgc tgaacgactc tatctacaac atctctgaag gttacaacat caacaacctg     1140

aaagttaact tccgtggtca gaacgctaac ctgaacccgc gtatcatcac cccgatcacc     1200

ggtcgtggtc tggttaaaaa aatcatccgt ttctgccaca aagcgattga tggccgctct     1260

ctctataaca aaacgctgga ttgcatcgaa atcaacaacg gtgaactgtt cttcgttgct     1320

tctgaaaact cttacaacga cgacaacatc aacaccccga aagaaatcga cgacaccgtt     1380

acctctaaca acaactacga aaacgacctg gaccaggtta tcctgaactt caactctgaa     1440

tctgctccgg gtctgtctga cgaaaaactg aacctgacca tccagaacga cgcttacatc     1500

ccgaaatacg actctaacgg tacctctgac atcgaacagc acgacgttaa cgaactgaac     1560

gttttcttct acctggacgc tcagaaagtt ccggaaggtg aaaacaacgt taacctgacc     1620

tcttctatcg acaccgctct gctggaacag ccgaaaatct acaccttctt ctcttctgag     1680

ttcatcaaca acgttaacaa accggttcag gctgctctgt tcgtttcttg gattcagcag     1740

gttctggttg acttcaccac cgaagctaac cagaaatcta ccgttgacaa aatcgctgac     1800

atctctatcg ttgttccgta catcggtctg gctctgaaca tcggtaacga agctcagaaa     1860

ggtaacttca aagacgctct ggaactgctg ggtgctggta tcctgctgga gttcgaaccg     1920

gaactgctga tcccgaccat cctggttttc accatcaaat ctttcctggg ttcttctgac     1980

aacaaaaaca aagttatcaa agctatcaac aacgctctga aagaacgtga cgaaaaatgg     2040

aaagaagttt actctttcat cgtttctaac tggatgacca aaatcaacac ccagttcaac     2100

aaacgtaaag aacagatgta ccaggctctc cagaaccagg ttaacgctat caaaaccatc     2160

atcgaatcta aatacaactc ttacaccctg gaagaaaaaa acgaactgac caacaaatac     2220

gacatcaaac agatcgaaaa cgaactgaac cagaaagttt ctatcgctat gaacaacatc     2280

gaccgtttcc tgaccgaatc ttctatctct tacctgatga aactcatcaa cgaagttaaa     2340

atcaacaaac tgcgtgaata cgacgaaaac gttaaaacct acctgctgaa ctacatcatc     2400

cagcacggtt ctatcctggg tgaatctcag caggaactga actctatggt taccgacacc     2460

ctgaacaact ctatcccgtt caaactgtct tcttacaccg acgacaaaat cctgatctct     2520

tacttcaaca aattctttaa acgcattaag agttcatcgg ttctgaatat gcggtacaaa     2580

aatgataaat atgtcgatac ttctggatat gatagcaata tcaacattaa cggcgacgtg     2640

tataaatatc cgacaaataa aaaccagttt gggatatata acgacaagct gtcggaggtc     2700

aatatttctc aaaacgacta tatcatttac gataataaat ataaaaactt tagcattagt     2760

ttttgggttc gtatacctaa ttatgacaat aaaattgtaa atgtgaataa cgagtatacc     2820

attataaact gtatgcgcga caataacagt ggttggaagg tatcgctgaa ccataatgag     2880

attatctgga ccctgcagga taatgcaggt ataaaccaga aactggcttt taactatgga     2940

aacgcaaatg ggatctcaga ttacattaat aaatggattt ttgttaccat tacgaacgat     3000

cgcttaggcg actcaaaact ttatattaat ggcaatctga tagatcagaa atcaatctta     3060

aatttgggca atattcatgt ctctgataac atcttgttca agatcgttaa ttgcagttac     3120

actcgttata ttggcattcg ttactttaat atcttcgata aagaactgga cgagacggaa     3180

atccagactc tgtattcaaa cgagcccaat actaatatat tgaaagattt ttggggtaac     3240

tatcttttat atgataaaga atactatctc ctgaatgtat tgaagccaaa caatttcata     3300

gatagacgca aggatagcac attaagtatc aacaatatca gatctactat actgttagca     3360

aatcgcctct actccggtat taaagtgaag attcagcggg ttaataactc cagtaccaat     3420

gataatctgg tccgtaagaa cgatcaggta tacatcaatt tcgtcgcgag caaaactcat     3480

ctcttcccgc tttacgccga tacagctacg acaaacaagg aaaaaaccat aaaaatttcc     3540

agctccggaa acagattcaa tcaagtagtt gtaatgaact ctgtgggtaa taattgtacg     3600

atgaacttta agaataacaa tgggaacaat attggacttt tgggcttcaa agccgacaca     3660

gtggtggcgt ccacctggta ttacacgcac atgcgggacc atacgaattc gaacggttgc     3720

ttctggaact ttatctcgga agaacacggg tggcaagaaa aa                        3762


<210>  11
<211>  1254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BoNT/E with a C1 Activation Loop

<400>  11

Met Pro Lys Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp Arg 
1               5                   10                  15      


Thr Ile Leu Tyr Ile Lys Pro Gly Gly Cys Gln Glu Phe Tyr Lys Ser 
            20                  25                  30          


Phe Asn Ile Met Lys Asn Ile Trp Ile Ile Pro Glu Arg Asn Val Ile 
        35                  40                  45              


Gly Thr Thr Pro Gln Asp Phe His Pro Pro Thr Ser Leu Lys Asn Gly 
    50                  55                  60                  


Asp Ser Ser Tyr Tyr Asp Pro Asn Tyr Leu Gln Ser Asp Glu Glu Lys 
65                  70                  75                  80  


Asp Arg Phe Leu Lys Ile Val Thr Lys Ile Phe Asn Arg Ile Asn Asn 
                85                  90                  95      


Asn Leu Ser Gly Gly Ile Leu Leu Glu Glu Leu Ser Lys Ala Asn Pro 
            100                 105                 110         


Tyr Leu Gly Asn Asp Asn Thr Pro Asp Asn Gln Phe His Ile Gly Asp 
        115                 120                 125             


Ala Ser Ala Val Glu Ile Lys Phe Ser Asn Gly Ser Gln Asp Ile Leu 
    130                 135                 140                 


Leu Pro Asn Val Ile Ile Met Gly Ala Glu Pro Asp Leu Phe Glu Thr 
145                 150                 155                 160 


Asn Ser Ser Asn Ile Ser Leu Arg Asn Asn Tyr Met Pro Ser Asn His 
                165                 170                 175     


Gly Phe Gly Ser Ile Ala Ile Val Thr Phe Ser Pro Glu Tyr Ser Phe 
            180                 185                 190         


Arg Phe Asn Asp Asn Ser Met Asn Glu Phe Ile Gln Asp Pro Ala Leu 
        195                 200                 205             


Thr Leu Met His Gln Leu Ile Tyr Ser Leu His Gly Leu Tyr Gly Ala 
    210                 215                 220                 


Lys Gly Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn Pro Leu 
225                 230                 235                 240 


Ile Thr Asn Ile Arg Gly Thr Asn Ile Glu Glu Phe Leu Thr Phe Gly 
                245                 250                 255     


Gly Thr Asp Leu Asn Ile Ile Thr Ser Ala Gln Ser Asn Asp Ile Tyr 
            260                 265                 270         


Thr Asn Leu Leu Ala Asp Tyr Lys Lys Ile Ala Ser Lys Leu Ser Lys 
        275                 280                 285             


Val Gln Val Ser Asn Pro Leu Leu Asn Pro Tyr Lys Asp Val Phe Glu 
    290                 295                 300                 


Ala Lys Tyr Gly Leu Asp Lys Asp Ala Ser Gly Ile Tyr Ser Val Asn 
305                 310                 315                 320 


Ile Asn Lys Phe Asn Asp Ile Phe Lys Lys Leu Tyr Ser Phe Thr Glu 
                325                 330                 335     


Phe Asp Leu Ala Thr Lys Phe Gln Val Lys Cys Arg Gln Thr Tyr Ile 
            340                 345                 350         


Gly Gln Tyr Lys Tyr Phe Lys Leu Ser Asn Leu Leu Asn Asp Ser Ile 
        355                 360                 365             


Tyr Asn Ile Ser Glu Gly Tyr Asn Ile Asn Asn Leu Lys Val Asn Phe 
    370                 375                 380                 


Arg Gly Gln Asn Ala Asn Leu Asn Pro Arg Ile Ile Thr Pro Ile Thr 
385                 390                 395                 400 


Gly Arg Gly Leu Val Lys Lys Ile Ile Arg Phe Cys His Lys Ala Ile 
                405                 410                 415     


Asp Gly Arg Ser Leu Tyr Asn Lys Thr Leu Asp Cys Ile Glu Ile Asn 
            420                 425                 430         


Asn Gly Glu Leu Phe Phe Val Ala Ser Glu Asn Ser Tyr Asn Asp Asp 
        435                 440                 445             


Asn Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Val Thr Ser Asn Asn 
    450                 455                 460                 


Asn Tyr Glu Asn Asp Leu Asp Gln Val Ile Leu Asn Phe Asn Ser Glu 
465                 470                 475                 480 


Ser Ala Pro Gly Leu Ser Asp Glu Lys Leu Asn Leu Thr Ile Gln Asn 
                485                 490                 495     


Asp Ala Tyr Ile Pro Lys Tyr Asp Ser Asn Gly Thr Ser Asp Ile Glu 
            500                 505                 510         


Gln His Asp Val Asn Glu Leu Asn Val Phe Phe Tyr Leu Asp Ala Gln 
        515                 520                 525             


Lys Val Pro Glu Gly Glu Asn Asn Val Asn Leu Thr Ser Ser Ile Asp 
    530                 535                 540                 


Thr Ala Leu Leu Glu Gln Pro Lys Ile Tyr Thr Phe Phe Ser Ser Glu 
545                 550                 555                 560 


Phe Ile Asn Asn Val Asn Lys Pro Val Gln Ala Ala Leu Phe Val Ser 
                565                 570                 575     


Trp Ile Gln Gln Val Leu Val Asp Phe Thr Thr Glu Ala Asn Gln Lys 
            580                 585                 590         


Ser Thr Val Asp Lys Ile Ala Asp Ile Ser Ile Val Val Pro Tyr Ile 
        595                 600                 605             


Gly Leu Ala Leu Asn Ile Gly Asn Glu Ala Gln Lys Gly Asn Phe Lys 
    610                 615                 620                 


Asp Ala Leu Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Glu Pro 
625                 630                 635                 640 


Glu Leu Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe Leu 
                645                 650                 655     


Gly Ser Ser Asp Asn Lys Asn Lys Val Ile Lys Ala Ile Asn Asn Ala 
            660                 665                 670         


Leu Lys Glu Arg Asp Glu Lys Trp Lys Glu Val Tyr Ser Phe Ile Val 
        675                 680                 685             


Ser Asn Trp Met Thr Lys Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu 
    690                 695                 700                 


Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asn Ala Ile Lys Thr Ile 
705                 710                 715                 720 


Ile Glu Ser Lys Tyr Asn Ser Tyr Thr Leu Glu Glu Lys Asn Glu Leu 
                725                 730                 735     


Thr Asn Lys Tyr Asp Ile Lys Gln Ile Glu Asn Glu Leu Asn Gln Lys 
            740                 745                 750         


Val Ser Ile Ala Met Asn Asn Ile Asp Arg Phe Leu Thr Glu Ser Ser 
        755                 760                 765             


Ile Ser Tyr Leu Met Lys Leu Ile Asn Glu Val Lys Ile Asn Lys Leu 
    770                 775                 780                 


Arg Glu Tyr Asp Glu Asn Val Lys Thr Tyr Leu Leu Asn Tyr Ile Ile 
785                 790                 795                 800 


Gln His Gly Ser Ile Leu Gly Glu Ser Gln Gln Glu Leu Asn Ser Met 
                805                 810                 815     


Val Thr Asp Thr Leu Asn Asn Ser Ile Pro Phe Lys Leu Ser Ser Tyr 
            820                 825                 830         


Thr Asp Asp Lys Ile Leu Ile Ser Tyr Phe Asn Lys Phe Phe Lys Arg 
        835                 840                 845             


Ile Lys Ser Ser Ser Val Leu Asn Met Arg Tyr Lys Asn Asp Lys Tyr 
    850                 855                 860                 


Val Asp Thr Ser Gly Tyr Asp Ser Asn Ile Asn Ile Asn Gly Asp Val 
865                 870                 875                 880 


Tyr Lys Tyr Pro Thr Asn Lys Asn Gln Phe Gly Ile Tyr Asn Asp Lys 
                885                 890                 895     


Leu Ser Glu Val Asn Ile Ser Gln Asn Asp Tyr Ile Ile Tyr Asp Asn 
            900                 905                 910         


Lys Tyr Lys Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Asn Tyr 
        915                 920                 925             


Asp Asn Lys Ile Val Asn Val Asn Asn Glu Tyr Thr Ile Ile Asn Cys 
    930                 935                 940                 


Met Arg Asp Asn Asn Ser Gly Trp Lys Val Ser Leu Asn His Asn Glu 
945                 950                 955                 960 


Ile Ile Trp Thr Leu Gln Asp Asn Ala Gly Ile Asn Gln Lys Leu Ala 
                965                 970                 975     


Phe Asn Tyr Gly Asn Ala Asn Gly Ile Ser Asp Tyr Ile Asn Lys Trp 
            980                 985                 990         


Ile Phe Val Thr Ile Thr Asn Asp  Arg Leu Gly Asp Ser  Lys Leu Tyr 
        995                 1000                 1005             


Ile Asn  Gly Asn Leu Ile Asp  Gln Lys Ser Ile Leu  Asn Leu Gly 
    1010                 1015                 1020             


Asn Ile  His Val Ser Asp Asn  Ile Leu Phe Lys Ile  Val Asn Cys 
    1025                 1030                 1035             


Ser Tyr  Thr Arg Tyr Ile Gly  Ile Arg Tyr Phe Asn  Ile Phe Asp 
    1040                 1045                 1050             


Lys Glu  Leu Asp Glu Thr Glu  Ile Gln Thr Leu Tyr  Ser Asn Glu 
    1055                 1060                 1065             


Pro Asn  Thr Asn Ile Leu Lys  Asp Phe Trp Gly Asn  Tyr Leu Leu 
    1070                 1075                 1080             


Tyr Asp  Lys Glu Tyr Tyr Leu  Leu Asn Val Leu Lys  Pro Asn Asn 
    1085                 1090                 1095             


Phe Ile  Asp Arg Arg Lys Asp  Ser Thr Leu Ser Ile  Asn Asn Ile 
    1100                 1105                 1110             


Arg Ser  Thr Ile Leu Leu Ala  Asn Arg Leu Tyr Ser  Gly Ile Lys 
    1115                 1120                 1125             


Val Lys  Ile Gln Arg Val Asn  Asn Ser Ser Thr Asn  Asp Asn Leu 
    1130                 1135                 1140             


Val Arg  Lys Asn Asp Gln Val  Tyr Ile Asn Phe Val  Ala Ser Lys 
    1145                 1150                 1155             


Thr His  Leu Phe Pro Leu Tyr  Ala Asp Thr Ala Thr  Thr Asn Lys 
    1160                 1165                 1170             


Glu Lys  Thr Ile Lys Ile Ser  Ser Ser Gly Asn Arg  Phe Asn Gln 
    1175                 1180                 1185             


Val Val  Val Met Asn Ser Val  Gly Asn Asn Cys Thr  Met Asn Phe 
    1190                 1195                 1200             


Lys Asn  Asn Asn Gly Asn Asn  Ile Gly Leu Leu Gly  Phe Lys Ala 
    1205                 1210                 1215             


Asp Thr  Val Val Ala Ser Thr  Trp Tyr Tyr Thr His  Met Arg Asp 
    1220                 1225                 1230             


His Thr  Asn Ser Asn Gly Cys  Phe Trp Asn Phe Ile  Ser Glu Glu 
    1235                 1240                 1245             


His Gly  Trp Gln Glu Lys 
    1250                 


<210>  12
<211>  3867
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BoNT/A1C1 with a C1 Activation Loop

<400>  12
atgccattcg tcaacaagca attcaactac aaagacccag tcaacggcgt cgacatcgca       60

tacatcaaga ttccgaacgc cggtcaaatg cagccggtta aggcttttaa gatccacaac      120

aagatttggg ttatcccgga gcgtgacacc ttcacgaacc cggaagaagg cgatctgaac      180

ccgccaccgg aagcgaagca agtccctgtc agctactacg attcgacgta cctgagcacg      240

gataacgaaa aagataacta cctgaaaggt gtgaccaagc tgttcgaacg tatctacagc      300

acggatctgg gtcgcatgct gctgactagc attgttcgcg gtatcccgtt ctggggtggt      360

agcacgattg acaccgaact gaaggttatc gacactaact gcattaacgt tattcaaccg      420

gatggtagct atcgtagcga agagctgaat ctggtcatca ttggcccgag cgcagacatt      480

atccaattcg agtgcaagag ctttggtcac gaggttctga atctgacccg caatggctat      540

ggtagcaccc agtacattcg tttttcgccg gattttacct tcggctttga agagagcctg      600

gaggttgata ccaatccgtt gctgggtgcg ggcaaattcg ctaccgatcc ggctgtcacg      660

ctggcccatg aactgatcca cgcaggccac cgcctgtacg gcattgccat caacccaaac      720

cgtgtgttca aggttaatac gaatgcatac tacgagatga gcggcctgga agtcagcttc      780

gaagaactgc gcaccttcgg tggccatgac gctaaattca ttgacagctt gcaagagaat      840

gagttccgtc tgtactacta taacaaattc aaagacattg caagcacgtt gaacaaggcc      900

aaaagcatcg ttggtactac cgcgtcgttg cagtatatga agaatgtgtt taaagagaag      960

tacctgctgt ccgaggatac ctccggcaag tttagcgttg ataagctgaa gtttgacaaa     1020

ctgtacaaga tgctgaccga gatttacacc gaggacaact ttgtgaaatt cttcaaagtg     1080

ttgaatcgta aaacctatct gaattttgac aaagcggttt tcaagattaa catcgtgccg     1140

aaggtgaact acaccatcta tgacggtttt aacctgcgta acaccaacct ggcggcgaac     1200

tttaacggtc agaatacgga aatcaacaac atgaatttca cgaagttgaa gaacttcacg     1260

ggtctgttcg agttctataa gctgctgtgc cacaaagcga ttgatggccg ctctctctat     1320

aacaaaacgc tggattgcat taaggtaaac aattgggatc tgttcttttc gccatccgaa     1380

gataatttta ccaacgacct gaacaagggt gaagaaatca ccagcgatac gaatattgaa     1440

gcagcggaag agaatatcag cctggatctg atccagcagt actatctgac ctttaacttc     1500

gacaatgaac cggagaacat tagcattgag aatctgagca gcgacattat cggtcagctg     1560

gaactgatgc cgaatatcga acgtttcccg aacggcaaaa agtacgagct ggacaagtac     1620

actatgttcc attacctgcg tgcacaggag tttgaacacg gtaaaagccg tatcgcgctg     1680

accaacagcg ttaacgaggc cctgctgaac ccgagccgtg tctatacctt cttcagcagc     1740

gactatgtta agaaagtgaa caaagccact gaggccgcga tgttcctggg ctgggtggaa     1800

cagctggtat atgacttcac ggacgagacg agcgaagtga gcactaccga caaaattgct     1860

gatattacca tcattatccc gtatattggt ccggcactga acattggcaa catgctgtac     1920

aaagacgatt ttgtgggtgc cctgatcttc tccggtgccg tgattctgct ggagttcatt     1980

ccggagattg cgatcccggt gttgggtacc ttcgcgctgg tgtcctacat cgcgaataag     2040

gttctgacgg ttcagaccat cgataacgcg ctgtcgaaac gtaatgaaaa atgggacgag     2100

gtttacaaat acattgttac gaattggctg gcgaaagtca atacccagat cgacctgatc     2160

cgtaagaaaa tgaaagaggc gctggagaat caggcggagg ccaccaaagc aattatcaac     2220

taccaataca accagtacac ggaagaagag aagaataaca ttaacttcaa tatcgatgat     2280

ttgagcagca agctgaatga atctatcaac aaagcgatga tcaatatcaa caagtttttg     2340

aatcagtgta gcgtttcgta cctgatgaat agcatgattc cgtatggcgt caaacgtctg     2400

gaggacttcg acgccagcct gaaagatgcg ttgctgaaat acatttacga caatcgtggt     2460

acgctgattg gccaagttga ccgcttgaaa gacaaagtta acaataccct gagcaccgac     2520

atcccatttc aactgagcaa gtatgttgat aatcaacgtc tgttgagcac tttcaccgag     2580

tatatcaaaa acattaatga cagcaaaatt ctgagcctgc agaatcgtaa gaatacgctg     2640

gtagatacca gtggatataa tgcggaagtc tcagaagagg gtgatgtaca gctgaacccg     2700

atctttccgt tcgactttaa actggggtct agtggtgaag atcgcggtaa agtgatcgtt     2760

acccaaaacg agaacattgt gtataacagc atgtacgaga gtttctcaat ttctttctgg     2820

attcgcatca ataaatgggt ttctaatttg cctggctata ccatcattga tagcgtcaaa     2880

aacaactcgg gctggtcgat tggcattatt agcaactttc tggtgtttac cctgaaacag     2940

aatgaggatt cggaacagag cattaacttc tcctacgaca tcagcaacaa tgcaccaggg     3000

tataacaaat ggttcttcgt aacggtgacg aacaatatga tgggcaatat gaaaatctac     3060

attaacggga aacttatcga caccattaaa gtgaaagagc ttactgggat caattttagt     3120

aaaaccatta cctttgagat caacaaaatt ccggacacgg gtctgattac ctccgattcg     3180

gataatatca atatgtggat tcgcgacttt tatatcttcg ccaaagaact tgatggcaaa     3240

gatatcaaca ttttgtttaa ttccctgcag tataccaatg tcgttaagga ctattggggc     3300

aatgatctcc gctacaataa agaatactac atggttaaca tcgactatct caatcgctac     3360

atgtatgcta actcgcgtca aattgtgttt aacacacgtc gtaacaacaa cgattttaac     3420

gaaggttata aaatcattat caaacggatc cgcggcaata cgaacgatac tcgtgttcgt     3480

ggcggtgaca ttctgtattt cgacatgacg attaataata aagcgtacaa tctgttcatg     3540

aagaacgaaa ccatgtacgc cgataaccat tccactgaag atatctacgc aatcggactt     3600

cgcgaacaga ccaaagacat taacgacaac atcatctttc agattcaacc gatgaataat     3660

acctactact atgcctccca gatcttcaaa agtaatttca acggcgaaaa catttcaggc     3720

atttgctcaa tcggcactta tcggttccgg ttaggtggtg attggtatcg tcacaactac     3780

cttgttccca cagtgaaaca aggcaactat gcatcgctct tagaaagcac atctacgcat     3840

tggggttttg tgccagtcag tgaataa                                         3867


<210>  13
<211>  1288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BoNT/A1C1 with a C1 Activation Loop

<400>  13

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys His Lys 
            420                 425                 430         


Ala Ile Asp Gly Arg Ser Leu Tyr Asn Lys Thr Leu Asp Cys Ile Lys 
        435                 440                 445             


Val Asn Asn Trp Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr 
    450                 455                 460                 


Asn Asp Leu Asn Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu 
465                 470                 475                 480 


Ala Ala Glu Glu Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu 
                485                 490                 495     


Thr Phe Asn Phe Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu 
            500                 505                 510         


Ser Ser Asp Ile Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg 
        515                 520                 525             


Phe Pro Asn Gly Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His 
    530                 535                 540                 


Tyr Leu Arg Ala Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu 
545                 550                 555                 560 


Thr Asn Ser Val Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr 
                565                 570                 575     


Phe Phe Ser Ser Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala 
            580                 585                 590         


Ala Met Phe Leu Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp 
        595                 600                 605             


Glu Thr Ser Glu Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile 
    610                 615                 620                 


Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr 
625                 630                 635                 640 


Lys Asp Asp Phe Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu 
                645                 650                 655     


Leu Glu Phe Ile Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala 
            660                 665                 670         


Leu Val Ser Tyr Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp 
        675                 680                 685             


Asn Ala Leu Ser Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr 
    690                 695                 700                 


Ile Val Thr Asn Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile 
705                 710                 715                 720 


Arg Lys Lys Met Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys 
                725                 730                 735     


Ala Ile Ile Asn Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn 
            740                 745                 750         


Asn Ile Asn Phe Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser 
        755                 760                 765             


Ile Asn Lys Ala Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser 
    770                 775                 780                 


Val Ser Tyr Leu Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu 
785                 790                 795                 800 


Glu Asp Phe Asp Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr 
                805                 810                 815     


Asp Asn Arg Gly Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys 
            820                 825                 830         


Val Asn Asn Thr Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr 
        835                 840                 845             


Val Asp Asn Gln Arg Leu Leu Ser Thr Phe Thr Glu Tyr Ile Lys Asn 
    850                 855                 860                 


Ile Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Arg Lys Asn Thr Leu 
865                 870                 875                 880 


Val Asp Thr Ser Gly Tyr Asn Ala Glu Val Ser Glu Glu Gly Asp Val 
                885                 890                 895     


Gln Leu Asn Pro Ile Phe Pro Phe Asp Phe Lys Leu Gly Ser Ser Gly 
            900                 905                 910         


Glu Asp Arg Gly Lys Val Ile Val Thr Gln Asn Glu Asn Ile Val Tyr 
        915                 920                 925             


Asn Ser Met Tyr Glu Ser Phe Ser Ile Ser Phe Trp Ile Arg Ile Asn 
    930                 935                 940                 


Lys Trp Val Ser Asn Leu Pro Gly Tyr Thr Ile Ile Asp Ser Val Lys 
945                 950                 955                 960 


Asn Asn Ser Gly Trp Ser Ile Gly Ile Ile Ser Asn Phe Leu Val Phe 
                965                 970                 975     


Thr Leu Lys Gln Asn Glu Asp Ser Glu Gln Ser Ile Asn Phe Ser Tyr 
            980                 985                 990         


Asp Ile Ser Asn Asn Ala Pro Gly  Tyr Asn Lys Trp Phe  Phe Val Thr 
        995                 1000                 1005             


Val Thr  Asn Asn Met Met Gly  Asn Met Lys Ile Tyr  Ile Asn Gly 
    1010                 1015                 1020             


Lys Leu  Ile Asp Thr Ile Lys  Val Lys Glu Leu Thr  Gly Ile Asn 
    1025                 1030                 1035             


Phe Ser  Lys Thr Ile Thr Phe  Glu Ile Asn Lys Ile  Pro Asp Thr 
    1040                 1045                 1050             


Gly Leu  Ile Thr Ser Asp Ser  Asp Asn Ile Asn Met  Trp Ile Arg 
    1055                 1060                 1065             


Asp Phe  Tyr Ile Phe Ala Lys  Glu Leu Asp Gly Lys  Asp Ile Asn 
    1070                 1075                 1080             


Ile Leu  Phe Asn Ser Leu Gln  Tyr Thr Asn Val Val  Lys Asp Tyr 
    1085                 1090                 1095             


Trp Gly  Asn Asp Leu Arg Tyr  Asn Lys Glu Tyr Tyr  Met Val Asn 
    1100                 1105                 1110             


Ile Asp  Tyr Leu Asn Arg Tyr  Met Tyr Ala Asn Ser  Arg Gln Ile 
    1115                 1120                 1125             


Val Phe  Asn Thr Arg Arg Asn  Asn Asn Asp Phe Asn  Glu Gly Tyr 
    1130                 1135                 1140             


Lys Ile  Ile Ile Lys Arg Ile  Arg Gly Asn Thr Asn  Asp Thr Arg 
    1145                 1150                 1155             


Val Arg  Gly Gly Asp Ile Leu  Tyr Phe Asp Met Thr  Ile Asn Asn 
    1160                 1165                 1170             


Lys Ala  Tyr Asn Leu Phe Met  Lys Asn Glu Thr Met  Tyr Ala Asp 
    1175                 1180                 1185             


Asn His  Ser Thr Glu Asp Ile  Tyr Ala Ile Gly Leu  Arg Glu Gln 
    1190                 1195                 1200             


Thr Lys  Asp Ile Asn Asp Asn  Ile Ile Phe Gln Ile  Gln Pro Met 
    1205                 1210                 1215             


Asn Asn  Thr Tyr Tyr Tyr Ala  Ser Gln Ile Phe Lys  Ser Asn Phe 
    1220                 1225                 1230             


Asn Gly  Glu Asn Ile Ser Gly  Ile Cys Ser Ile Gly  Thr Tyr Arg 
    1235                 1240                 1245             


Phe Arg  Leu Gly Gly Asp Trp  Tyr Arg His Asn Tyr  Leu Val Pro 
    1250                 1255                 1260             


Thr Val  Lys Gln Gly Asn Tyr  Ala Ser Leu Leu Glu  Ser Thr Ser 
    1265                 1270                 1275             


Thr His  Trp Gly Phe Val Pro  Val Ser Glu 
    1280                 1285             


<210>  14
<211>  3878
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BoNT/C1(0) (Endonegative)

<400>  14
atgccgatca cgattaataa tttcaactat agcgatccgg tggacaataa gaatattctg       60

tatctggata ctcatctgaa tacgctggct aacgaaccgg agaaagcgtt ccgcatcaca      120

ggcaacatct gggttattcc cgatcgcttt tcacgcaaca gcaaccctaa tctgaacaaa      180

cctcctcgtg tcaccagtcc taaatccggt tattacgacc caaactatct gagtacggat      240

agcgataaag atccctttct gaaagagatc attaagctgt tcaaacgcat taactctcgc      300

gaaattgggg aagagctgat ctatcggctt tcgacagata tcccgttccc aggtaacaat      360

aataccccga ttaatacttt cgactttgat gttgatttca attctgtgga tgtgaaaacg      420

cgtcaaggca ataattgggt gaaaactggt agcattaacc cgagtgtaat tatcacaggt      480

ccccgtgaga acatcatcga cccggaaacc tctaccttca agctgacgaa caacacgttt      540

gctgcacagg aagggtttgg tgccctgtca atcatttcca tctcaccgcg tttcatgtta      600

acctactcca atgccacaaa tgatgttggc gaaggacgtt ttagcaaatc agaattttgc      660

atggacccaa ttctcattct gatgggcacg ctgaacaatg cgatgcacaa cttgtatggc      720

attgctattc caaacgatca aaccattagc tccgttacca gtaatatctt ctatagccag      780

tataatgtca aattggagta tgccgaaatt tacgcctttg gaggcccgac cattgacctg      840

attccgaaat ctgcacgcaa atacttcgaa gaaaaggcgt tagattacta tcgcagcatc      900

gcgaaacgcc tgaactcgat taccacggcc aatccgtcgt cgttcaacaa atacattggt      960

gaatataaac agaaactgat tcgcaaatat cggtttgtcg tagaaagctc tggtgaagtg     1020

actgtaaacc gcaacaaatt tgtcgaactc tacaacgagt tgacccaaat ctttaccgag     1080

tttaactacg caaagatcta taacgtacag aaccgcaaga tttatcttag caatgtatac     1140

acaccggtta ctgcgaacat cttagacgac aatgtgtatg atattcagaa tggctttaac     1200

atcccgaaat caaatctgaa cgttctgttt atgggccaga acctgagtcg taatccagca     1260

ctgcgtaaag tgaacccgga aaatatgctc tacttgttta ccaaattttg ccacaaagcg     1320

attgatggcc gctctctcta taacaaaacg ctggattgtc gtgagttact tgtgaagaac     1380

actgatttac cgttcattgg ggatatctcc gacgtgaaaa ccgatatctt cctgcgcaaa     1440

gacattaatg aagaaacgga agtcatctat taccccgaca atgtgagcgt tgatcaggtc     1500

attttatcga agaacacctc cgaacatggt cagttggatt tgctgtaccc tagcattgac     1560

tcggagagtg aaatccttcc gggcgaaaat caagtgtttt acgacaaccg tacccaaaat     1620

gttgattatt tgaattctta ttactacctg gaatctcaga aattgagcga caatgtggaa     1680

gatttcacgt tcacacgctc cattgaggaa gcgctggata atagcgcgaa agtgtatacg     1740

tatttcccta ccttggcgaa taaagtaaat gctggtgtcc agggaggctt atttctgatg     1800

tgggcgaatg atgtggtaga agattttacg accaatattt tgcgtaagga caccttagat     1860

aaaattagcg atgttagcgc catcatcccc tatattggcc cagcactgaa tatctcgaac     1920

tctgtgcgtc gcggaaactt caccgaagca tttgcggtga ccggggttac tattctgttg     1980

gaagcctttc cggagtttac tattccggcg ctgggtgcgt ttgtgattta ttcgaaagta     2040

caagaacgca atgaaattat caaaaccatc gataattgcc tggaacaacg cattaaacgc     2100

tggaaggatt cttatgaatg gatgatgggc acctggttat cccgtattat cacacagttt     2160

aacaacatct cgtatcagat gtacgattca ctgaactacc aagcaggggc gatcaaagcc     2220

aagatcgact tagaatacaa gaaatattca ggtagcgata aagagaatat taaaagccag     2280

gttgaaaacc tgaagaactc tctggatgtc aaaatttcag aggctatgaa caacattaac     2340

aaatttatcc gcgaatgtag cgtcacgtat ctgtttaaaa acatgctccc gaaagtgatt     2400

gatgagctca acgagtttga tcgcaacaca aaggccaaac tgattaacct gattgatagt     2460

cacaatatta ttttagtcgg tgaagttgac aagctgaagg ctaaggtcaa taacagcttt     2520

cagaacacta ttccgtttaa tattttctcc tatacgaaca atagtctgct gaaagacatt     2580

atcaacgaat acttcaacaa tattaatgac agcaaaattc tgagcctgca gaatcgtaag     2640

aatacgctgg tagataccag tggatataat gcggaagtct cagaagaggg tgatgtacag     2700

ctgaacccga tctttccgtt cgactttaaa ctggggtcta gtggtgaaga tcgcggtaaa     2760

gtgatcgtta cccaaaacga gaacattgtg tataacagca tgtacgagag tttctcaatt     2820

tctttctgga ttcgcatcaa taaatgggtt tctaatttgc ctggctatac catcattgat     2880

agcgtcaaaa acaactcggg ctggtcgatt ggcattatta gcaactttct ggtgtttacc     2940

ctgaaacaga atgaggattc ggaacagagc attaacttct cctacgacat cagcaacaat     3000

gcaccagggt ataacaaatg gttcttcgta acggtgacga acaatatgat gggcaatatg     3060

aaaatctaca ttaacgggaa acttatcgac accattaaag tgaaagagct tactgggatc     3120

aattttagta aaaccattac ctttgagatc aacaaaattc cggacacggg tctgattacc     3180

tccgattcgg ataatatcaa tatgtggatt cgcgactttt atatcttcgc caaagaactt     3240

gatggcaaag atatcaacat tttgtttaat tccctgcagt ataccaatgt cgttaaggac     3300

tattggggca atgatctccg ctacaataaa gaatactaca tggttaacat cgactatctc     3360

aatcgctaca tgtatgctaa ctcgcgtcaa attgtgttta acacacgtcg taacaacaac     3420

gattttaacg aaggttataa aatcattatc aaacggatcc gcggcaatac gaacgatact     3480

cgtgttcgtg gcggtgacat tctgtatttc gacatgacga ttaataataa agcgtacaat     3540

ctgttcatga agaacgaaac catgtacgcc gataaccatt ccactgaaga tatctacgca     3600

atcggacttc gcgaacagac caaagacatt aacgacaaca tcatctttca gattcaaccg     3660

atgaataata cctactacta tgcctcccag atcttcaaaa gtaatttcaa cggcgaaaac     3720

atttcaggca tttgctcaat cggcacttat cggttccggt taggtggtga ttggtatcgt     3780

cacaactacc ttgttcccac agtgaaacaa ggcaactatg catcgctctt agaaagcaca     3840

tctacgcatt ggggttttgt gccagtcagt gaataatg                             3878


<210>  15
<211>  1291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BoNT/C1(0) (Endonegative)

<400>  15

Met Pro Ile Thr Ile Asn Asn Phe Asn Tyr Ser Asp Pro Val Asp Asn 
1               5                   10                  15      


Lys Asn Ile Leu Tyr Leu Asp Thr His Leu Asn Thr Leu Ala Asn Glu 
            20                  25                  30          


Pro Glu Lys Ala Phe Arg Ile Thr Gly Asn Ile Trp Val Ile Pro Asp 
        35                  40                  45              


Arg Phe Ser Arg Asn Ser Asn Pro Asn Leu Asn Lys Pro Pro Arg Val 
    50                  55                  60                  


Thr Ser Pro Lys Ser Gly Tyr Tyr Asp Pro Asn Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Ser Asp Lys Asp Pro Phe Leu Lys Glu Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Ser Arg Glu Ile Gly Glu Glu Leu Ile Tyr Arg Leu Ser Thr 
            100                 105                 110         


Asp Ile Pro Phe Pro Gly Asn Asn Asn Thr Pro Ile Asn Thr Phe Asp 
        115                 120                 125             


Phe Asp Val Asp Phe Asn Ser Val Asp Val Lys Thr Arg Gln Gly Asn 
    130                 135                 140                 


Asn Trp Val Lys Thr Gly Ser Ile Asn Pro Ser Val Ile Ile Thr Gly 
145                 150                 155                 160 


Pro Arg Glu Asn Ile Ile Asp Pro Glu Thr Ser Thr Phe Lys Leu Thr 
                165                 170                 175     


Asn Asn Thr Phe Ala Ala Gln Glu Gly Phe Gly Ala Leu Ser Ile Ile 
            180                 185                 190         


Ser Ile Ser Pro Arg Phe Met Leu Thr Tyr Ser Asn Ala Thr Asn Asp 
        195                 200                 205             


Val Gly Glu Gly Arg Phe Ser Lys Ser Glu Phe Cys Met Asp Pro Ile 
    210                 215                 220                 


Leu Ile Leu Met Gly Thr Leu Asn Asn Ala Met His Asn Leu Tyr Gly 
225                 230                 235                 240 


Ile Ala Ile Pro Asn Asp Gln Thr Ile Ser Ser Val Thr Ser Asn Ile 
                245                 250                 255     


Phe Tyr Ser Gln Tyr Asn Val Lys Leu Glu Tyr Ala Glu Ile Tyr Ala 
            260                 265                 270         


Phe Gly Gly Pro Thr Ile Asp Leu Ile Pro Lys Ser Ala Arg Lys Tyr 
        275                 280                 285             


Phe Glu Glu Lys Ala Leu Asp Tyr Tyr Arg Ser Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Ser Ile Thr Thr Ala Asn Pro Ser Ser Phe Asn Lys Tyr Ile Gly 
305                 310                 315                 320 


Glu Tyr Lys Gln Lys Leu Ile Arg Lys Tyr Arg Phe Val Val Glu Ser 
                325                 330                 335     


Ser Gly Glu Val Thr Val Asn Arg Asn Lys Phe Val Glu Leu Tyr Asn 
            340                 345                 350         


Glu Leu Thr Gln Ile Phe Thr Glu Phe Asn Tyr Ala Lys Ile Tyr Asn 
        355                 360                 365             


Val Gln Asn Arg Lys Ile Tyr Leu Ser Asn Val Tyr Thr Pro Val Thr 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Val Tyr Asp Ile Gln Asn Gly Phe Asn 
385                 390                 395                 400 


Ile Pro Lys Ser Asn Leu Asn Val Leu Phe Met Gly Gln Asn Leu Ser 
                405                 410                 415     


Arg Asn Pro Ala Leu Arg Lys Val Asn Pro Glu Asn Met Leu Tyr Leu 
            420                 425                 430         


Phe Thr Lys Phe Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn 
        435                 440                 445             


Lys Thr Leu Asp Cys Arg Glu Leu Leu Val Lys Asn Thr Asp Leu Pro 
    450                 455                 460                 


Phe Ile Gly Asp Ile Ser Asp Val Lys Thr Asp Ile Phe Leu Arg Lys 
465                 470                 475                 480 


Asp Ile Asn Glu Glu Thr Glu Val Ile Tyr Tyr Pro Asp Asn Val Ser 
                485                 490                 495     


Val Asp Gln Val Ile Leu Ser Lys Asn Thr Ser Glu His Gly Gln Leu 
            500                 505                 510         


Asp Leu Leu Tyr Pro Ser Ile Asp Ser Glu Ser Glu Ile Leu Pro Gly 
        515                 520                 525             


Glu Asn Gln Val Phe Tyr Asp Asn Arg Thr Gln Asn Val Asp Tyr Leu 
    530                 535                 540                 


Asn Ser Tyr Tyr Tyr Leu Glu Ser Gln Lys Leu Ser Asp Asn Val Glu 
545                 550                 555                 560 


Asp Phe Thr Phe Thr Arg Ser Ile Glu Glu Ala Leu Asp Asn Ser Ala 
                565                 570                 575     


Lys Val Tyr Thr Tyr Phe Pro Thr Leu Ala Asn Lys Val Asn Ala Gly 
            580                 585                 590         


Val Gln Gly Gly Leu Phe Leu Met Trp Ala Asn Asp Val Val Glu Asp 
        595                 600                 605             


Phe Thr Thr Asn Ile Leu Arg Lys Asp Thr Leu Asp Lys Ile Ser Asp 
    610                 615                 620                 


Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Ser Asn 
625                 630                 635                 640 


Ser Val Arg Arg Gly Asn Phe Thr Glu Ala Phe Ala Val Thr Gly Val 
                645                 650                 655     


Thr Ile Leu Leu Glu Ala Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly 
            660                 665                 670         


Ala Phe Val Ile Tyr Ser Lys Val Gln Glu Arg Asn Glu Ile Ile Lys 
        675                 680                 685             


Thr Ile Asp Asn Cys Leu Glu Gln Arg Ile Lys Arg Trp Lys Asp Ser 
    690                 695                 700                 


Tyr Glu Trp Met Met Gly Thr Trp Leu Ser Arg Ile Ile Thr Gln Phe 
705                 710                 715                 720 


Asn Asn Ile Ser Tyr Gln Met Tyr Asp Ser Leu Asn Tyr Gln Ala Gly 
                725                 730                 735     


Ala Ile Lys Ala Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser 
            740                 745                 750         


Asp Lys Glu Asn Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu 
        755                 760                 765             


Asp Val Lys Ile Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg 
    770                 775                 780                 


Glu Cys Ser Val Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile 
785                 790                 795                 800 


Asp Glu Leu Asn Glu Phe Asp Arg Asn Thr Lys Ala Lys Leu Ile Asn 
                805                 810                 815     


Leu Ile Asp Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Lys Leu 
            820                 825                 830         


Lys Ala Lys Val Asn Asn Ser Phe Gln Asn Thr Ile Pro Phe Asn Ile 
        835                 840                 845             


Phe Ser Tyr Thr Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr 
    850                 855                 860                 


Phe Asn Asn Ile Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Arg Lys 
865                 870                 875                 880 


Asn Thr Leu Val Asp Thr Ser Gly Tyr Asn Ala Glu Val Ser Glu Glu 
                885                 890                 895     


Gly Asp Val Gln Leu Asn Pro Ile Phe Pro Phe Asp Phe Lys Leu Gly 
            900                 905                 910         


Ser Ser Gly Glu Asp Arg Gly Lys Val Ile Val Thr Gln Asn Glu Asn 
        915                 920                 925             


Ile Val Tyr Asn Ser Met Tyr Glu Ser Phe Ser Ile Ser Phe Trp Ile 
    930                 935                 940                 


Arg Ile Asn Lys Trp Val Ser Asn Leu Pro Gly Tyr Thr Ile Ile Asp 
945                 950                 955                 960 


Ser Val Lys Asn Asn Ser Gly Trp Ser Ile Gly Ile Ile Ser Asn Phe 
                965                 970                 975     


Leu Val Phe Thr Leu Lys Gln Asn Glu Asp Ser Glu Gln Ser Ile Asn 
            980                 985                 990         


Phe Ser Tyr Asp Ile Ser Asn Asn  Ala Pro Gly Tyr Asn  Lys Trp Phe 
        995                 1000                 1005             


Phe Val  Thr Val Thr Asn Asn  Met Met Gly Asn Met  Lys Ile Tyr 
    1010                 1015                 1020             


Ile Asn  Gly Lys Leu Ile Asp  Thr Ile Lys Val Lys  Glu Leu Thr 
    1025                 1030                 1035             


Gly Ile  Asn Phe Ser Lys Thr  Ile Thr Phe Glu Ile  Asn Lys Ile 
    1040                 1045                 1050             


Pro Asp  Thr Gly Leu Ile Thr  Ser Asp Ser Asp Asn  Ile Asn Met 
    1055                 1060                 1065             


Trp Ile  Arg Asp Phe Tyr Ile  Phe Ala Lys Glu Leu  Asp Gly Lys 
    1070                 1075                 1080             


Asp Ile  Asn Ile Leu Phe Asn  Ser Leu Gln Tyr Thr  Asn Val Val 
    1085                 1090                 1095             


Lys Asp  Tyr Trp Gly Asn Asp  Leu Arg Tyr Asn Lys  Glu Tyr Tyr 
    1100                 1105                 1110             


Met Val  Asn Ile Asp Tyr Leu  Asn Arg Tyr Met Tyr  Ala Asn Ser 
    1115                 1120                 1125             


Arg Gln  Ile Val Phe Asn Thr  Arg Arg Asn Asn Asn  Asp Phe Asn 
    1130                 1135                 1140             


Glu Gly  Tyr Lys Ile Ile Ile  Lys Arg Ile Arg Gly  Asn Thr Asn 
    1145                 1150                 1155             


Asp Thr  Arg Val Arg Gly Gly  Asp Ile Leu Tyr Phe  Asp Met Thr 
    1160                 1165                 1170             


Ile Asn  Asn Lys Ala Tyr Asn  Leu Phe Met Lys Asn  Glu Thr Met 
    1175                 1180                 1185             


Tyr Ala  Asp Asn His Ser Thr  Glu Asp Ile Tyr Ala  Ile Gly Leu 
    1190                 1195                 1200             


Arg Glu  Gln Thr Lys Asp Ile  Asn Asp Asn Ile Ile  Phe Gln Ile 
    1205                 1210                 1215             


Gln Pro  Met Asn Asn Thr Tyr  Tyr Tyr Ala Ser Gln  Ile Phe Lys 
    1220                 1225                 1230             


Ser Asn  Phe Asn Gly Glu Asn  Ile Ser Gly Ile Cys  Ser Ile Gly 
    1235                 1240                 1245             


Thr Tyr  Arg Phe Arg Leu Gly  Gly Asp Trp Tyr Arg  His Asn Tyr 
    1250                 1255                 1260             


Leu Val  Pro Thr Val Lys Gln  Gly Asn Tyr Ala Ser  Leu Leu Glu 
    1265                 1270                 1275             


Ser Thr  Ser Thr His Trp Gly  Phe Val Pro Val Ser  Glu 
    1280                 1285                 1290     


<210>  16
<211>  3876
<212>  DNA
<213>  Clostridium botulinum

<400>  16
atgccgatca cgattaataa tttcaactat agcgatccgg tggacaataa gaatattctg       60

tatctggata ctcatctgaa tacgctggct aacgaaccgg agaaagcgtt ccgcatcaca      120

ggcaacatct gggttattcc cgatcgcttt tcacgcaaca gcaaccctaa tctgaacaaa      180

cctcctcgtg tcaccagtcc taaatccggt tattacgacc caaactatct gagtacggat      240

agcgataaag atccctttct gaaagagatc attaagctgt tcaaacgcat taactctcgc      300

gaaattgggg aagagctgat ctatcggctt tcgacagata tcccgttccc aggtaacaat      360

aataccccga ttaatacttt cgactttgat gttgatttca attctgtgga tgtgaaaacg      420

cgtcaaggca ataattgggt gaaaactggt agcattaacc cgagtgtaat tatcacaggt      480

ccccgtgaga acatcatcga cccggaaacc tctaccttca agctgacgaa caacacgttt      540

gctgcacagg aagggtttgg tgccctgtca atcatttcca tctcaccgcg tttcatgtta      600

acctactcca atgccacaaa tgatgttggc gaaggacgtt ttagcaaatc agaattttgc      660

atggacccaa ttctcattct gatgcacgag ctgaaccatg cgatgcacaa cttgtatggc      720

attgctattc caaacgatca aaccattagc tccgttacca gtaatatctt ctatagccag      780

tataatgtca aattggagta tgccgaaatt tacgcctttg gaggcccgac cattgacctg      840

attccgaaat ctgcacgcaa atacttcgaa gaaaaggcgt tagattacta tcgcagcatc      900

gcgaaacgcc tgaactcgat taccacggcc aatccgtcgt cgttcaacaa atacattggt      960

gaatataaac agaaactgat tcgcaaatat cggtttgtcg tagaaagctc tggtgaagtg     1020

actgtaaacc gcaacaaatt tgtcgaactc tacaacgagt tgacccaaat ctttaccgag     1080

tttaactacg caaagatcta taacgtacag aaccgcaaga tttatcttag caatgtatac     1140

acaccggtta ctgcgaacat cttagacgac aatgtgtatg atattcagaa tggctttaac     1200

atcccgaaat caaatctgaa cgttctgttt atgggccaga acctgagtcg taatccagca     1260

ctgcgtaaag tgaacccgga aaatatgctc tacttgttta ccaaattttg ccacaaagcg     1320

attgatggcc gctctctcta taacaaaacg ctggattgtc gtgagttact tgtgaagaac     1380

actgatttac cgttcattgg ggatatctcc gacgtgaaaa ccgatatctt cctgcgcaaa     1440

gacattaatg aagaaacgga agtcatctat taccccgaca atgtgagcgt tgatcaggtc     1500

attttatcga agaacacctc cgaacatggt cagttggatt tgctgtaccc tagcattgac     1560

tcggagagtg aaatccttcc gggcgaaaat caagtgtttt acgacaaccg tacccaaaat     1620

gttgattatt tgaattctta ttactacctg gaatctcaga aattgagcga caatgtggaa     1680

gatttcacgt tcacacgctc cattgaggaa gcgctggata atagcgcgaa agtgtatacg     1740

tatttcccta ccttggcgaa taaagtaaat gctggtgtcc agggaggctt atttctgatg     1800

tgggcgaatg atgtggtaga agattttacg accaatattt tgcgtaagga caccttagat     1860

aaaattagcg atgttagcgc catcatcccc tatattggcc cagcactgaa tatctcgaac     1920

tctgtgcgtc gcggaaactt caccgaagca tttgcggtga ccggggttac tattctgttg     1980

gaagcctttc cggagtttac tattccggcg ctgggtgcgt ttgtgattta ttcgaaagta     2040

caagaacgca atgaaattat caaaaccatc gataattgcc tggaacaacg cattaaacgc     2100

tggaaggatt cttatgaatg gatgatgggc acctggttat cccgtattat cacacagttt     2160

aacaacatct cgtatcagat gtacgattca ctgaactacc aagcaggggc gatcaaagcc     2220

aagatcgact tagaatacaa gaaatattca ggtagcgata aagagaatat taaaagccag     2280

gttgaaaacc tgaagaactc tctggatgtc aaaatttcag aggctatgaa caacattaac     2340

aaatttatcc gcgaatgtag cgtcacgtat ctgtttaaaa acatgctccc gaaagtgatt     2400

gatgagctca acgagtttga tcgcaacaca aaggccaaac tgattaacct gattgatagt     2460

cacaatatta ttttagtcgg tgaagttgac aagctgaagg ctaaggtcaa taacagcttt     2520

cagaacacta ttccgtttaa tattttctcc tatacgaaca atagtctgct gaaagacatt     2580

atcaacgaat acttcaacaa tattaatgac agcaaaattc tgagcctgca gaatcgtaag     2640

aatacgctgg tagataccag tggatataat gcggaagtct cagaagaggg tgatgtacag     2700

ctgaacccga tctttccgtt cgactttaaa ctggggtcta gtggtgaaga tcgcggtaaa     2760

gtgatcgtta cccaaaacga gaacattgtg tataacagca tgtacgagag tttctcaatt     2820

tctttctgga ttcgcatcaa taaatgggtt tctaatttgc ctggctatac catcattgat     2880

agcgtcaaaa acaactcggg ctggtcgatt ggcattatta gcaactttct ggtgtttacc     2940

ctgaaacaga atgaggattc ggaacagagc attaacttct cctacgacat cagcaacaat     3000

gcaccagggt ataacaaatg gttcttcgta acggtgacga acaatatgat gggcaatatg     3060

aaaatctaca ttaacgggaa acttatcgac accattaaag tgaaagagct tactgggatc     3120

aattttagta aaaccattac ctttgagatc aacaaaattc cggacacggg tctgattacc     3180

tccgattcgg ataatatcaa tatgtggatt cgcgactttt atatcttcgc caaagaactt     3240

gatggcaaag atatcaacat tttgtttaat tccctgcagt ataccaatgt cgttaaggac     3300

tattggggca atgatctccg ctacaataaa gaatactaca tggttaacat cgactatctc     3360

aatcgctaca tgtatgctaa ctcgcgtcaa attgtgttta acacacgtcg taacaacaac     3420

gattttaacg aaggttataa aatcattatc aaacggatcc gcggcaatac gaacgatact     3480

cgtgttcgtg gcggtgacat tctgtatttc gacatgacga ttaataataa agcgtacaat     3540

ctgttcatga agaacgaaac catgtacgcc gataaccatt ccactgaaga tatctacgca     3600

atcggacttc gcgaacagac caaagacatt aacgacaaca tcatctttca gattcaaccg     3660

atgaataata cctactacta tgcctcccag atcttcaaaa gtaatttcaa cggcgaaaac     3720

atttcaggca tttgctcaat cggcacttat cggttccggt taggtggtga ttggtatcgt     3780

cacaactacc ttgttcccac agtgaaacaa ggcaactatg catcgctctt agaaagcaca     3840

tctacgcatt ggggttttgt gccagtcagt gaataa                               3876


<210>  17
<211>  1291
<212>  PRT
<213>  Clostridium botulinum

<400>  17

Met Pro Ile Thr Ile Asn Asn Phe Asn Tyr Ser Asp Pro Val Asp Asn 
1               5                   10                  15      


Lys Asn Ile Leu Tyr Leu Asp Thr His Leu Asn Thr Leu Ala Asn Glu 
            20                  25                  30          


Pro Glu Lys Ala Phe Arg Ile Thr Gly Asn Ile Trp Val Ile Pro Asp 
        35                  40                  45              


Arg Phe Ser Arg Asn Ser Asn Pro Asn Leu Asn Lys Pro Pro Arg Val 
    50                  55                  60                  


Thr Ser Pro Lys Ser Gly Tyr Tyr Asp Pro Asn Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Ser Asp Lys Asp Pro Phe Leu Lys Glu Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Ser Arg Glu Ile Gly Glu Glu Leu Ile Tyr Arg Leu Ser Thr 
            100                 105                 110         


Asp Ile Pro Phe Pro Gly Asn Asn Asn Thr Pro Ile Asn Thr Phe Asp 
        115                 120                 125             


Phe Asp Val Asp Phe Asn Ser Val Asp Val Lys Thr Arg Gln Gly Asn 
    130                 135                 140                 


Asn Trp Val Lys Thr Gly Ser Ile Asn Pro Ser Val Ile Ile Thr Gly 
145                 150                 155                 160 


Pro Arg Glu Asn Ile Ile Asp Pro Glu Thr Ser Thr Phe Lys Leu Thr 
                165                 170                 175     


Asn Asn Thr Phe Ala Ala Gln Glu Gly Phe Gly Ala Leu Ser Ile Ile 
            180                 185                 190         


Ser Ile Ser Pro Arg Phe Met Leu Thr Tyr Ser Asn Ala Thr Asn Asp 
        195                 200                 205             


Val Gly Glu Gly Arg Phe Ser Lys Ser Glu Phe Cys Met Asp Pro Ile 
    210                 215                 220                 


Leu Ile Leu Met His Glu Leu Asn His Ala Met His Asn Leu Tyr Gly 
225                 230                 235                 240 


Ile Ala Ile Pro Asn Asp Gln Thr Ile Ser Ser Val Thr Ser Asn Ile 
                245                 250                 255     


Phe Tyr Ser Gln Tyr Asn Val Lys Leu Glu Tyr Ala Glu Ile Tyr Ala 
            260                 265                 270         


Phe Gly Gly Pro Thr Ile Asp Leu Ile Pro Lys Ser Ala Arg Lys Tyr 
        275                 280                 285             


Phe Glu Glu Lys Ala Leu Asp Tyr Tyr Arg Ser Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Ser Ile Thr Thr Ala Asn Pro Ser Ser Phe Asn Lys Tyr Ile Gly 
305                 310                 315                 320 


Glu Tyr Lys Gln Lys Leu Ile Arg Lys Tyr Arg Phe Val Val Glu Ser 
                325                 330                 335     


Ser Gly Glu Val Thr Val Asn Arg Asn Lys Phe Val Glu Leu Tyr Asn 
            340                 345                 350         


Glu Leu Thr Gln Ile Phe Thr Glu Phe Asn Tyr Ala Lys Ile Tyr Asn 
        355                 360                 365             


Val Gln Asn Arg Lys Ile Tyr Leu Ser Asn Val Tyr Thr Pro Val Thr 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Val Tyr Asp Ile Gln Asn Gly Phe Asn 
385                 390                 395                 400 


Ile Pro Lys Ser Asn Leu Asn Val Leu Phe Met Gly Gln Asn Leu Ser 
                405                 410                 415     


Arg Asn Pro Ala Leu Arg Lys Val Asn Pro Glu Asn Met Leu Tyr Leu 
            420                 425                 430         


Phe Thr Lys Phe Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn 
        435                 440                 445             


Lys Thr Leu Asp Cys Arg Glu Leu Leu Val Lys Asn Thr Asp Leu Pro 
    450                 455                 460                 


Phe Ile Gly Asp Ile Ser Asp Val Lys Thr Asp Ile Phe Leu Arg Lys 
465                 470                 475                 480 


Asp Ile Asn Glu Glu Thr Glu Val Ile Tyr Tyr Pro Asp Asn Val Ser 
                485                 490                 495     


Val Asp Gln Val Ile Leu Ser Lys Asn Thr Ser Glu His Gly Gln Leu 
            500                 505                 510         


Asp Leu Leu Tyr Pro Ser Ile Asp Ser Glu Ser Glu Ile Leu Pro Gly 
        515                 520                 525             


Glu Asn Gln Val Phe Tyr Asp Asn Arg Thr Gln Asn Val Asp Tyr Leu 
    530                 535                 540                 


Asn Ser Tyr Tyr Tyr Leu Glu Ser Gln Lys Leu Ser Asp Asn Val Glu 
545                 550                 555                 560 


Asp Phe Thr Phe Thr Arg Ser Ile Glu Glu Ala Leu Asp Asn Ser Ala 
                565                 570                 575     


Lys Val Tyr Thr Tyr Phe Pro Thr Leu Ala Asn Lys Val Asn Ala Gly 
            580                 585                 590         


Val Gln Gly Gly Leu Phe Leu Met Trp Ala Asn Asp Val Val Glu Asp 
        595                 600                 605             


Phe Thr Thr Asn Ile Leu Arg Lys Asp Thr Leu Asp Lys Ile Ser Asp 
    610                 615                 620                 


Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Ser Asn 
625                 630                 635                 640 


Ser Val Arg Arg Gly Asn Phe Thr Glu Ala Phe Ala Val Thr Gly Val 
                645                 650                 655     


Thr Ile Leu Leu Glu Ala Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly 
            660                 665                 670         


Ala Phe Val Ile Tyr Ser Lys Val Gln Glu Arg Asn Glu Ile Ile Lys 
        675                 680                 685             


Thr Ile Asp Asn Cys Leu Glu Gln Arg Ile Lys Arg Trp Lys Asp Ser 
    690                 695                 700                 


Tyr Glu Trp Met Met Gly Thr Trp Leu Ser Arg Ile Ile Thr Gln Phe 
705                 710                 715                 720 


Asn Asn Ile Ser Tyr Gln Met Tyr Asp Ser Leu Asn Tyr Gln Ala Gly 
                725                 730                 735     


Ala Ile Lys Ala Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser 
            740                 745                 750         


Asp Lys Glu Asn Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu 
        755                 760                 765             


Asp Val Lys Ile Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg 
    770                 775                 780                 


Glu Cys Ser Val Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile 
785                 790                 795                 800 


Asp Glu Leu Asn Glu Phe Asp Arg Asn Thr Lys Ala Lys Leu Ile Asn 
                805                 810                 815     


Leu Ile Asp Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Lys Leu 
            820                 825                 830         


Lys Ala Lys Val Asn Asn Ser Phe Gln Asn Thr Ile Pro Phe Asn Ile 
        835                 840                 845             


Phe Ser Tyr Thr Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr 
    850                 855                 860                 


Phe Asn Asn Ile Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Arg Lys 
865                 870                 875                 880 


Asn Thr Leu Val Asp Thr Ser Gly Tyr Asn Ala Glu Val Ser Glu Glu 
                885                 890                 895     


Gly Asp Val Gln Leu Asn Pro Ile Phe Pro Phe Asp Phe Lys Leu Gly 
            900                 905                 910         


Ser Ser Gly Glu Asp Arg Gly Lys Val Ile Val Thr Gln Asn Glu Asn 
        915                 920                 925             


Ile Val Tyr Asn Ser Met Tyr Glu Ser Phe Ser Ile Ser Phe Trp Ile 
    930                 935                 940                 


Arg Ile Asn Lys Trp Val Ser Asn Leu Pro Gly Tyr Thr Ile Ile Asp 
945                 950                 955                 960 


Ser Val Lys Asn Asn Ser Gly Trp Ser Ile Gly Ile Ile Ser Asn Phe 
                965                 970                 975     


Leu Val Phe Thr Leu Lys Gln Asn Glu Asp Ser Glu Gln Ser Ile Asn 
            980                 985                 990         


Phe Ser Tyr Asp Ile Ser Asn Asn  Ala Pro Gly Tyr Asn  Lys Trp Phe 
        995                 1000                 1005             


Phe Val  Thr Val Thr Asn Asn  Met Met Gly Asn Met  Lys Ile Tyr 
    1010                 1015                 1020             


Ile Asn  Gly Lys Leu Ile Asp  Thr Ile Lys Val Lys  Glu Leu Thr 
    1025                 1030                 1035             


Gly Ile  Asn Phe Ser Lys Thr  Ile Thr Phe Glu Ile  Asn Lys Ile 
    1040                 1045                 1050             


Pro Asp  Thr Gly Leu Ile Thr  Ser Asp Ser Asp Asn  Ile Asn Met 
    1055                 1060                 1065             


Trp Ile  Arg Asp Phe Tyr Ile  Phe Ala Lys Glu Leu  Asp Gly Lys 
    1070                 1075                 1080             


Asp Ile  Asn Ile Leu Phe Asn  Ser Leu Gln Tyr Thr  Asn Val Val 
    1085                 1090                 1095             


Lys Asp  Tyr Trp Gly Asn Asp  Leu Arg Tyr Asn Lys  Glu Tyr Tyr 
    1100                 1105                 1110             


Met Val  Asn Ile Asp Tyr Leu  Asn Arg Tyr Met Tyr  Ala Asn Ser 
    1115                 1120                 1125             


Arg Gln  Ile Val Phe Asn Thr  Arg Arg Asn Asn Asn  Asp Phe Asn 
    1130                 1135                 1140             


Glu Gly  Tyr Lys Ile Ile Ile  Lys Arg Ile Arg Gly  Asn Thr Asn 
    1145                 1150                 1155             


Asp Thr  Arg Val Arg Gly Gly  Asp Ile Leu Tyr Phe  Asp Met Thr 
    1160                 1165                 1170             


Ile Asn  Asn Lys Ala Tyr Asn  Leu Phe Met Lys Asn  Glu Thr Met 
    1175                 1180                 1185             


Tyr Ala  Asp Asn His Ser Thr  Glu Asp Ile Tyr Ala  Ile Gly Leu 
    1190                 1195                 1200             


Arg Glu  Gln Thr Lys Asp Ile  Asn Asp Asn Ile Ile  Phe Gln Ile 
    1205                 1210                 1215             


Gln Pro  Met Asn Asn Thr Tyr  Tyr Tyr Ala Ser Gln  Ile Phe Lys 
    1220                 1225                 1230             


Ser Asn  Phe Asn Gly Glu Asn  Ile Ser Gly Ile Cys  Ser Ile Gly 
    1235                 1240                 1245             


Thr Tyr  Arg Phe Arg Leu Gly  Gly Asp Trp Tyr Arg  His Asn Tyr 
    1250                 1255                 1260             


Leu Val  Pro Thr Val Lys Gln  Gly Asn Tyr Ala Ser  Leu Leu Glu 
    1265                 1270                 1275             


Ser Thr  Ser Thr His Trp Gly  Phe Val Pro Val Ser  Glu 
    1280                 1285                 1290     


<210>  18
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Enterokinase and Factor Xa Cleavage Site

<400>  18

Ile Asp Gly Arg 
1               


<210>  19
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Enterokinase and Factor Xa Cleavage Site Variant

<400>  19

Ile Glu Gly Arg 
1               


<210>  20
<211>  45
<212>  PRT
<213>  Clostridium botulinum

<400>  20

Cys Pro Arg Asn Gly Leu Leu Tyr Asn Ala Ile Tyr Arg Asn Ser Lys 
1               5                   10                  15      


Asn Tyr Leu Asn Asn Ile Asp Leu Glu Asp Lys Lys Thr Thr Ser Lys 
            20                  25                  30          


Thr Asn Val Ser Tyr Pro Cys Ser Leu Leu Asn Gly Cys 
        35                  40                  45  


<210>  21
<211>  25
<212>  PRT
<213>  Clostridium botulinum

<400>  21

Cys Val Arg Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly 
1               5                   10                  15      


Tyr Asn Lys Ala Leu Asn Asp Leu Cys 
            20                  25  


<210>  22
<211>  10
<212>  PRT
<213>  Clostridium botulinum

<400>  22

Cys Lys Ser Val Arg Ala Pro Gly Ile Cys 
1               5                   10  


<210>  23
<211>  14
<212>  PRT
<213>  Clostridium botulinum

<400>  23

Cys Leu Arg Leu Thr Lys Asn Ser Arg Asp Asp Ser Thr Cys 
1               5                   10                  


<210>  24
<211>  15
<212>  PRT
<213>  Clostridium botulinum

<400>  24

Cys Lys Asn Ile Val Ser Val Lys Gly Ile Arg Lys Ser Ile Cys 
1               5                   10                  15  


<210>  25
<211>  17
<212>  PRT
<213>  Clostridium botulinum

<400>  25

Cys Lys Ser Val Ile Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu 
1               5                   10                  15      


Cys 
    


<210>  26
<211>  17
<212>  PRT
<213>  Clostridium botulinum

<400>  26

Cys Lys Ser Ile Ile Pro Arg Lys Gly Thr Lys Gln Ser Pro Ser Leu 
1               5                   10                  15      


Cys 
    


<210>  27
<211>  17
<212>  PRT
<213>  Clostridium botulinum

<400>  27

Cys Lys Ser Ile Ile Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu 
1               5                   10                  15      


Cys 
    


<210>  28
<211>  15
<212>  PRT
<213>  Clostridium botulinum

<400>  28

Cys Leu Asn Ser Ser Phe Lys Lys Asn Thr Lys Lys Pro Leu Cys 
1               5                   10                  15  


<210>  29
<211>  15
<212>  PRT
<213>  Clostridium botulinum

<400>  29

Cys Lys Ser Ile Val Ser Lys Lys Gly Thr Lys Asn Ser Leu Cys 
1               5                   10                  15  


<210>  30
<211>  29
<212>  PRT
<213>  Clostridium tetani

<400>  30

Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile Arg Glu Asn Leu Tyr Asn 
1               5                   10                  15      


Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly Glu Leu Cys 
            20                  25                  


<210>  31
<211>  15
<212>  PRT
<213>  Clostridium botulinum

<400>  31

Cys Lys Pro Val Met Tyr Lys Asn Thr Gly Lys Ser Glu Gln Cys 
1               5                   10                  15  


<210>  32
<211>  3972
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild-Type BoNT/X-10HT

<400>  32
atgaaactgg aaatcaacaa attcaactac aacgatccga tcgatggcat taatgttatt       60

accatgcgtc cgcctcgtca tagcgataaa atcaataaag gtaaaggtcc gttcaaagcc      120

tttcaggtga ttaaaaacat ttggattgtg ccggaacgct acaactttac caataatacc      180

aacgatctga acattccgag cgaaccgatt atggaagcag atgccattta taacccgaac      240

tatctgaata ccccgagcga aaaagatgaa tttctgcagg gtgttatcaa agtgctggaa      300

cgcattaaaa gcaaaccgga aggtgaaaaa ctgctggaac tgattagcag cagcattccg      360

ctgccgctgg ttagcaatgg tgcactgacc ctgagcgata atgaaaccat tgcatatcaa      420

gagaacaaca acattgtgag caatctgcag gcaaacctgg ttatttatgg tccgggtcct      480

gatattgcaa ataatgcaac ctatggtctg tatagcaccc cgattagtaa tggtgaaggt      540

acactgagcg aagttagctt tagcccgttt tatctgaaac cgtttgatga aagctatggc      600

aattatcgta gcctggtgaa tatcgtgaac aaattcgtga aacgtgaatt tgcacctgat      660

ccggcaagca ccctgatgca tgaactggtt catgttaccc ataatctgta tggtattagc      720

aaccgcaact tctactataa ctttgacacc ggcaaaattg aaaccagccg tcagcagaat      780

agcctgattt ttgaagaact gctgaccttt ggtggcattg atagcaaagc aattagcagc      840

ctgatcatca agaaaattat cgaaaccgcc aagaacaact ataccacgct gattagcgaa      900

cgcctgaata ccgttaccgt tgaaaatgat ctgctgaaat atatcaaaaa caaaatcccg      960

gttcagggtc gtctgggtaa ctttaaactg gataccgcag aattcgagaa aaagctgaat     1020

accattctgt ttgtgctgaa cgaaagcaat ctggcacagc gttttagcat tctggttcgt     1080

aaacattacc tgaaagaacg tccgattgat ccgatttatg tgaacattct ggatgacaat     1140

agctacagca ccctggaagg ttttaacatt agcagtcagg gtagcaatga tttccaaggt     1200

cagctgctgg aaagcagcta ttttgaaaaa attgaaagca atgccctgcg tgccttcatt     1260

aaaatctgtc cgcgtaatgg tctgctgtat aatgccattt atcgcaacag caaaaactac     1320

ctgaacaaca ttgatctgga agataaaaag accacgagca aaaccaatgt tagctatccg     1380

tgtagcctgc tgaatggttg tattgaagtt gaaaacaaag acctgttcct gattagcaac     1440

aaagatagcc tgaacgatat taacctgagc gaagaaaaaa tcaaaccgga aaccaccgtg     1500

ttcttcaaag ataaactgcc tccgcaggat attacgctga gcaattatga ttttaccgaa     1560

gccaatagca ttccgagcat tagccagcag aacattctgg aacgtaatga agaactgtat     1620

gaaccgattc gcaatagcct gtttgaaatc aaaaccatct atgtggataa gctgaccacc     1680

tttcattttc tggaagccca gaatattgat gagagcattg atagcagcaa aattcgtgtt     1740

gaactgaccg atagcgttga tgaagcactg agcaatccga ataaagttta tagcccgttc     1800

aagaacatga gcaacaccat taatagcatt gaaaccggta ttaccagcac ctacatcttt     1860

tatcagtggc tgcgtagcat cgtgaaagat tttagtgatg aaaccggcaa aatcgacgtg     1920

attgataaaa gcagcgatac cctggcaatt gttccgtata ttggtccgct gctgaatatt     1980

ggtaatgata ttcgtcatgg cgattttgtg ggtgcaattg aactggcagg cattaccgca     2040

ctgctggaat atgttccgga atttaccatt ccgattctgg ttggtctgga agttattggt     2100

ggcgaactgg cacgtgaaca ggttgaagca attgttaata atgccctgga taaacgcgat     2160

cagaaatggg cagaagttta caatattacc aaagcacagt ggtggggcac cattcattta     2220

cagattaata cccgtctggc ccatacctat aaagccctga gccgtcaggc aaatgccatt     2280

aaaatgaata tggaatttca gctggccaac tacaaaggca acatcgatga taaagccaaa     2340

atcaaaaacg ccatcagcga aaccgaaatc ctgctgaaca aaagcgttga acaggcaatg     2400

aaaaacaccg agaaattcat gatcaaactg agcaacagct atctgaccaa agaaatgatt     2460

ccgaaagtgc aggataacct gaaaaatttc gatctggaaa ccaagaaaac cctggacaaa     2520

tttatcaaag agaaagagga cattctgggc accaatctga gcagcagcct gcgtcgtaaa     2580

gttagcattc gtctgaataa aaacattgcc ttcgacatca acgatatccc gtttagcgaa     2640

tttgatgatc tgatcaacca gtacaaaaac gagatcgaag attatgaagt gctgaatctg     2700

ggtgcagaag atgggaaaat caaagatctg agcggtacaa ccagcgatat caatattggt     2760

tcagatatcg aactggccga tggtcgtgaa aataaagcca ttaagattaa aggcagcgag     2820

aacagcacca tcaaaattgc aatgaacaaa tatctgcgtt ttagcgcgac cgataacttt     2880

agcattagct tttggatcaa acatccgaaa ccgaccaatc tgcttaataa cggtattgaa     2940

tataccctgg tcgagaactt taatcagcgt ggttggaaaa ttagcatcca ggatagcaaa     3000

ctgatttggt atctgcgcga tcacaataac agcatcaaaa tcgttacacc ggattatatt     3060

gcgtttaatg gctggaacct gattaccatt acaaacaatc gtagcaaagg cagcattgtg     3120

tatgtgaacg gtagcaaaat tgaagagaag gatattagca gcatctggaa taccgaagtg     3180

gatgatccga ttatctttcg cctgaaaaac aatcgcgata cccaggcgtt taccctgctg     3240

gatcagttta gcatttatcg gaaagaactg aaccagaacg aagtggtgaa actgtataac     3300

tactacttca acagcaacta cattcgcgat atttggggta atccgctgca gtacaacaaa     3360

aaatactatc tgcagaccca ggacaaacct ggtaaaggtc tgatccgcga atattggagc     3420

agctttggtt atgattatgt gattctgagc gatagcaaga cgattacctt tccgaataat     3480

atccgttatg gtgccctgta taacggcagc aaagttctga tcaaaaatag caaaaaactg     3540

gatggtctgg tgcgcaataa agatttcatt cagctggaaa tcgatggcta taatatgggt     3600

attagcgcag atcgctttaa cgaggatacc aactatattg gcaccaccta tggtacaacc     3660

catgatctga ccaccgattt tgaaattatt cagcgccaag agaaataccg caattattgt     3720

cagctgaaaa ccccgtataa catctttcat aaaagcggtc tgatgagcac cgaaaccagc     3780

aaaccgacct ttcatgatta tcgtgactgg gtttatagca gcgcatggta ttttcagaac     3840

tatgaaaatc tgaacctgcg caaacatacc aaaaccaact ggtattttat cccgaaagat     3900

gaaggttggg atgaagatct tgaagttctg tttcagggtc cgcatcatca ccaccatcac     3960

catcatcatc ac                                                         3972


<210>  33
<211>  1306
<212>  PRT
<213>  Clostridium botulinum

<400>  33

Met Lys Leu Glu Ile Asn Lys Phe Asn Tyr Asn Asp Pro Ile Asp Gly 
1               5                   10                  15      


Ile Asn Val Ile Thr Met Arg Pro Pro Arg His Ser Asp Lys Ile Asn 
            20                  25                  30          


Lys Gly Lys Gly Pro Phe Lys Ala Phe Gln Val Ile Lys Asn Ile Trp 
        35                  40                  45              


Ile Val Pro Glu Arg Tyr Asn Phe Thr Asn Asn Thr Asn Asp Leu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Pro Ile Met Glu Ala Asp Ala Ile Tyr Asn Pro Asn 
65                  70                  75                  80  


Tyr Leu Asn Thr Pro Ser Glu Lys Asp Glu Phe Leu Gln Gly Val Ile 
                85                  90                  95      


Lys Val Leu Glu Arg Ile Lys Ser Lys Pro Glu Gly Glu Lys Leu Leu 
            100                 105                 110         


Glu Leu Ile Ser Ser Ser Ile Pro Leu Pro Leu Val Ser Asn Gly Ala 
        115                 120                 125             


Leu Thr Leu Ser Asp Asn Glu Thr Ile Ala Tyr Gln Glu Asn Asn Asn 
    130                 135                 140                 


Ile Val Ser Asn Leu Gln Ala Asn Leu Val Ile Tyr Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Ile Ala Asn Asn Ala Thr Tyr Gly Leu Tyr Ser Thr Pro Ile Ser 
                165                 170                 175     


Asn Gly Glu Gly Thr Leu Ser Glu Val Ser Phe Ser Pro Phe Tyr Leu 
            180                 185                 190         


Lys Pro Phe Asp Glu Ser Tyr Gly Asn Tyr Arg Ser Leu Val Asn Ile 
        195                 200                 205             


Val Asn Lys Phe Val Lys Arg Glu Phe Ala Pro Asp Pro Ala Ser Thr 
    210                 215                 220                 


Leu Met His Glu Leu Val His Val Thr His Asn Leu Tyr Gly Ile Ser 
225                 230                 235                 240 


Asn Arg Asn Phe Tyr Tyr Asn Phe Asp Thr Gly Lys Ile Glu Thr Ser 
                245                 250                 255     


Arg Gln Gln Asn Ser Leu Ile Phe Glu Glu Leu Leu Thr Phe Gly Gly 
            260                 265                 270         


Ile Asp Ser Lys Ala Ile Ser Ser Leu Ile Ile Lys Lys Ile Ile Glu 
        275                 280                 285             


Thr Ala Lys Asn Asn Tyr Thr Thr Leu Ile Ser Glu Arg Leu Asn Thr 
    290                 295                 300                 


Val Thr Val Glu Asn Asp Leu Leu Lys Tyr Ile Lys Asn Lys Ile Pro 
305                 310                 315                 320 


Val Gln Gly Arg Leu Gly Asn Phe Lys Leu Asp Thr Ala Glu Phe Glu 
                325                 330                 335     


Lys Lys Leu Asn Thr Ile Leu Phe Val Leu Asn Glu Ser Asn Leu Ala 
            340                 345                 350         


Gln Arg Phe Ser Ile Leu Val Arg Lys His Tyr Leu Lys Glu Arg Pro 
        355                 360                 365             


Ile Asp Pro Ile Tyr Val Asn Ile Leu Asp Asp Asn Ser Tyr Ser Thr 
    370                 375                 380                 


Leu Glu Gly Phe Asn Ile Ser Ser Gln Gly Ser Asn Asp Phe Gln Gly 
385                 390                 395                 400 


Gln Leu Leu Glu Ser Ser Tyr Phe Glu Lys Ile Glu Ser Asn Ala Leu 
                405                 410                 415     


Arg Ala Phe Ile Lys Ile Cys Pro Arg Asn Gly Leu Leu Tyr Asn Ala 
            420                 425                 430         


Ile Tyr Arg Asn Ser Lys Asn Tyr Leu Asn Asn Ile Asp Leu Glu Asp 
        435                 440                 445             


Lys Lys Thr Thr Ser Lys Thr Asn Val Ser Tyr Pro Cys Ser Leu Leu 
    450                 455                 460                 


Asn Gly Cys Ile Glu Val Glu Asn Lys Asp Leu Phe Leu Ile Ser Asn 
465                 470                 475                 480 


Lys Asp Ser Leu Asn Asp Ile Asn Leu Ser Glu Glu Lys Ile Lys Pro 
                485                 490                 495     


Glu Thr Thr Val Phe Phe Lys Asp Lys Leu Pro Pro Gln Asp Ile Thr 
            500                 505                 510         


Leu Ser Asn Tyr Asp Phe Thr Glu Ala Asn Ser Ile Pro Ser Ile Ser 
        515                 520                 525             


Gln Gln Asn Ile Leu Glu Arg Asn Glu Glu Leu Tyr Glu Pro Ile Arg 
    530                 535                 540                 


Asn Ser Leu Phe Glu Ile Lys Thr Ile Tyr Val Asp Lys Leu Thr Thr 
545                 550                 555                 560 


Phe His Phe Leu Glu Ala Gln Asn Ile Asp Glu Ser Ile Asp Ser Ser 
                565                 570                 575     


Lys Ile Arg Val Glu Leu Thr Asp Ser Val Asp Glu Ala Leu Ser Asn 
            580                 585                 590         


Pro Asn Lys Val Tyr Ser Pro Phe Lys Asn Met Ser Asn Thr Ile Asn 
        595                 600                 605             


Ser Ile Glu Thr Gly Ile Thr Ser Thr Tyr Ile Phe Tyr Gln Trp Leu 
    610                 615                 620                 


Arg Ser Ile Val Lys Asp Phe Ser Asp Glu Thr Gly Lys Ile Asp Val 
625                 630                 635                 640 


Ile Asp Lys Ser Ser Asp Thr Leu Ala Ile Val Pro Tyr Ile Gly Pro 
                645                 650                 655     


Leu Leu Asn Ile Gly Asn Asp Ile Arg His Gly Asp Phe Val Gly Ala 
            660                 665                 670         


Ile Glu Leu Ala Gly Ile Thr Ala Leu Leu Glu Tyr Val Pro Glu Phe 
        675                 680                 685             


Thr Ile Pro Ile Leu Val Gly Leu Glu Val Ile Gly Gly Glu Leu Ala 
    690                 695                 700                 


Arg Glu Gln Val Glu Ala Ile Val Asn Asn Ala Leu Asp Lys Arg Asp 
705                 710                 715                 720 


Gln Lys Trp Ala Glu Val Tyr Asn Ile Thr Lys Ala Gln Trp Trp Gly 
                725                 730                 735     


Thr Ile His Leu Gln Ile Asn Thr Arg Leu Ala His Thr Tyr Lys Ala 
            740                 745                 750         


Leu Ser Arg Gln Ala Asn Ala Ile Lys Met Asn Met Glu Phe Gln Leu 
        755                 760                 765             


Ala Asn Tyr Lys Gly Asn Ile Asp Asp Lys Ala Lys Ile Lys Asn Ala 
    770                 775                 780                 


Ile Ser Glu Thr Glu Ile Leu Leu Asn Lys Ser Val Glu Gln Ala Met 
785                 790                 795                 800 


Lys Asn Thr Glu Lys Phe Met Ile Lys Leu Ser Asn Ser Tyr Leu Thr 
                805                 810                 815     


Lys Glu Met Ile Pro Lys Val Gln Asp Asn Leu Lys Asn Phe Asp Leu 
            820                 825                 830         


Glu Thr Lys Lys Thr Leu Asp Lys Phe Ile Lys Glu Lys Glu Asp Ile 
        835                 840                 845             


Leu Gly Thr Asn Leu Ser Ser Ser Leu Arg Arg Lys Val Ser Ile Arg 
    850                 855                 860                 


Leu Asn Lys Asn Ile Ala Phe Asp Ile Asn Asp Ile Pro Phe Ser Glu 
865                 870                 875                 880 


Phe Asp Asp Leu Ile Asn Gln Tyr Lys Asn Glu Ile Glu Asp Tyr Glu 
                885                 890                 895     


Val Leu Asn Leu Gly Ala Glu Asp Gly Lys Ile Lys Asp Leu Ser Gly 
            900                 905                 910         


Thr Thr Ser Asp Ile Asn Ile Gly Ser Asp Ile Glu Leu Ala Asp Gly 
        915                 920                 925             


Arg Glu Asn Lys Ala Ile Lys Ile Lys Gly Ser Glu Asn Ser Thr Ile 
    930                 935                 940                 


Lys Ile Ala Met Asn Lys Tyr Leu Arg Phe Ser Ala Thr Asp Asn Phe 
945                 950                 955                 960 


Ser Ile Ser Phe Trp Ile Lys His Pro Lys Pro Thr Asn Leu Leu Asn 
                965                 970                 975     


Asn Gly Ile Glu Tyr Thr Leu Val Glu Asn Phe Asn Gln Arg Gly Trp 
            980                 985                 990         


Lys Ile Ser Ile Gln Asp Ser Lys  Leu Ile Trp Tyr Leu  Arg Asp His 
        995                 1000                 1005             


Asn Asn  Ser Ile Lys Ile Val  Thr Pro Asp Tyr Ile  Ala Phe Asn 
    1010                 1015                 1020             


Gly Trp  Asn Leu Ile Thr Ile  Thr Asn Asn Arg Ser  Lys Gly Ser 
    1025                 1030                 1035             


Ile Val  Tyr Val Asn Gly Ser  Lys Ile Glu Glu Lys  Asp Ile Ser 
    1040                 1045                 1050             


Ser Ile  Trp Asn Thr Glu Val  Asp Asp Pro Ile Ile  Phe Arg Leu 
    1055                 1060                 1065             


Lys Asn  Asn Arg Asp Thr Gln  Ala Phe Thr Leu Leu  Asp Gln Phe 
    1070                 1075                 1080             


Ser Ile  Tyr Arg Lys Glu Leu  Asn Gln Asn Glu Val  Val Lys Leu 
    1085                 1090                 1095             


Tyr Asn  Tyr Tyr Phe Asn Ser  Asn Tyr Ile Arg Asp  Ile Trp Gly 
    1100                 1105                 1110             


Asn Pro  Leu Gln Tyr Asn Lys  Lys Tyr Tyr Leu Gln  Thr Gln Asp 
    1115                 1120                 1125             


Lys Pro  Gly Lys Gly Leu Ile  Arg Glu Tyr Trp Ser  Ser Phe Gly 
    1130                 1135                 1140             


Tyr Asp  Tyr Val Ile Leu Ser  Asp Ser Lys Thr Ile  Thr Phe Pro 
    1145                 1150                 1155             


Asn Asn  Ile Arg Tyr Gly Ala  Leu Tyr Asn Gly Ser  Lys Val Leu 
    1160                 1165                 1170             


Ile Lys  Asn Ser Lys Lys Leu  Asp Gly Leu Val Arg  Asn Lys Asp 
    1175                 1180                 1185             


Phe Ile  Gln Leu Glu Ile Asp  Gly Tyr Asn Met Gly  Ile Ser Ala 
    1190                 1195                 1200             


Asp Arg  Phe Asn Glu Asp Thr  Asn Tyr Ile Gly Thr  Thr Tyr Gly 
    1205                 1210                 1215             


Thr Thr  His Asp Leu Thr Thr  Asp Phe Glu Ile Ile  Gln Arg Gln 
    1220                 1225                 1230             


Glu Lys  Tyr Arg Asn Tyr Cys  Gln Leu Lys Thr Pro  Tyr Asn Ile 
    1235                 1240                 1245             


Phe His  Lys Ser Gly Leu Met  Ser Thr Glu Thr Ser  Lys Pro Thr 
    1250                 1255                 1260             


Phe His  Asp Tyr Arg Asp Trp  Val Tyr Ser Ser Ala  Trp Tyr Phe 
    1265                 1270                 1275             


Gln Asn  Tyr Glu Asn Leu Asn  Leu Arg Lys His Thr  Lys Thr Asn 
    1280                 1285                 1290             


Trp Tyr  Phe Ile Pro Lys Asp  Glu Gly Trp Asp Glu  Asp 
    1295                 1300                 1305     


<210>  34
<211>  1324
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wild-Type BoNT/X-10HT

<400>  34

Met Lys Leu Glu Ile Asn Lys Phe Asn Tyr Asn Asp Pro Ile Asp Gly 
1               5                   10                  15      


Ile Asn Val Ile Thr Met Arg Pro Pro Arg His Ser Asp Lys Ile Asn 
            20                  25                  30          


Lys Gly Lys Gly Pro Phe Lys Ala Phe Gln Val Ile Lys Asn Ile Trp 
        35                  40                  45              


Ile Val Pro Glu Arg Tyr Asn Phe Thr Asn Asn Thr Asn Asp Leu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Pro Ile Met Glu Ala Asp Ala Ile Tyr Asn Pro Asn 
65                  70                  75                  80  


Tyr Leu Asn Thr Pro Ser Glu Lys Asp Glu Phe Leu Gln Gly Val Ile 
                85                  90                  95      


Lys Val Leu Glu Arg Ile Lys Ser Lys Pro Glu Gly Glu Lys Leu Leu 
            100                 105                 110         


Glu Leu Ile Ser Ser Ser Ile Pro Leu Pro Leu Val Ser Asn Gly Ala 
        115                 120                 125             


Leu Thr Leu Ser Asp Asn Glu Thr Ile Ala Tyr Gln Glu Asn Asn Asn 
    130                 135                 140                 


Ile Val Ser Asn Leu Gln Ala Asn Leu Val Ile Tyr Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Ile Ala Asn Asn Ala Thr Tyr Gly Leu Tyr Ser Thr Pro Ile Ser 
                165                 170                 175     


Asn Gly Glu Gly Thr Leu Ser Glu Val Ser Phe Ser Pro Phe Tyr Leu 
            180                 185                 190         


Lys Pro Phe Asp Glu Ser Tyr Gly Asn Tyr Arg Ser Leu Val Asn Ile 
        195                 200                 205             


Val Asn Lys Phe Val Lys Arg Glu Phe Ala Pro Asp Pro Ala Ser Thr 
    210                 215                 220                 


Leu Met His Glu Leu Val His Val Thr His Asn Leu Tyr Gly Ile Ser 
225                 230                 235                 240 


Asn Arg Asn Phe Tyr Tyr Asn Phe Asp Thr Gly Lys Ile Glu Thr Ser 
                245                 250                 255     


Arg Gln Gln Asn Ser Leu Ile Phe Glu Glu Leu Leu Thr Phe Gly Gly 
            260                 265                 270         


Ile Asp Ser Lys Ala Ile Ser Ser Leu Ile Ile Lys Lys Ile Ile Glu 
        275                 280                 285             


Thr Ala Lys Asn Asn Tyr Thr Thr Leu Ile Ser Glu Arg Leu Asn Thr 
    290                 295                 300                 


Val Thr Val Glu Asn Asp Leu Leu Lys Tyr Ile Lys Asn Lys Ile Pro 
305                 310                 315                 320 


Val Gln Gly Arg Leu Gly Asn Phe Lys Leu Asp Thr Ala Glu Phe Glu 
                325                 330                 335     


Lys Lys Leu Asn Thr Ile Leu Phe Val Leu Asn Glu Ser Asn Leu Ala 
            340                 345                 350         


Gln Arg Phe Ser Ile Leu Val Arg Lys His Tyr Leu Lys Glu Arg Pro 
        355                 360                 365             


Ile Asp Pro Ile Tyr Val Asn Ile Leu Asp Asp Asn Ser Tyr Ser Thr 
    370                 375                 380                 


Leu Glu Gly Phe Asn Ile Ser Ser Gln Gly Ser Asn Asp Phe Gln Gly 
385                 390                 395                 400 


Gln Leu Leu Glu Ser Ser Tyr Phe Glu Lys Ile Glu Ser Asn Ala Leu 
                405                 410                 415     


Arg Ala Phe Ile Lys Ile Cys Pro Arg Asn Gly Leu Leu Tyr Asn Ala 
            420                 425                 430         


Ile Tyr Arg Asn Ser Lys Asn Tyr Leu Asn Asn Ile Asp Leu Glu Asp 
        435                 440                 445             


Lys Lys Thr Thr Ser Lys Thr Asn Val Ser Tyr Pro Cys Ser Leu Leu 
    450                 455                 460                 


Asn Gly Cys Ile Glu Val Glu Asn Lys Asp Leu Phe Leu Ile Ser Asn 
465                 470                 475                 480 


Lys Asp Ser Leu Asn Asp Ile Asn Leu Ser Glu Glu Lys Ile Lys Pro 
                485                 490                 495     


Glu Thr Thr Val Phe Phe Lys Asp Lys Leu Pro Pro Gln Asp Ile Thr 
            500                 505                 510         


Leu Ser Asn Tyr Asp Phe Thr Glu Ala Asn Ser Ile Pro Ser Ile Ser 
        515                 520                 525             


Gln Gln Asn Ile Leu Glu Arg Asn Glu Glu Leu Tyr Glu Pro Ile Arg 
    530                 535                 540                 


Asn Ser Leu Phe Glu Ile Lys Thr Ile Tyr Val Asp Lys Leu Thr Thr 
545                 550                 555                 560 


Phe His Phe Leu Glu Ala Gln Asn Ile Asp Glu Ser Ile Asp Ser Ser 
                565                 570                 575     


Lys Ile Arg Val Glu Leu Thr Asp Ser Val Asp Glu Ala Leu Ser Asn 
            580                 585                 590         


Pro Asn Lys Val Tyr Ser Pro Phe Lys Asn Met Ser Asn Thr Ile Asn 
        595                 600                 605             


Ser Ile Glu Thr Gly Ile Thr Ser Thr Tyr Ile Phe Tyr Gln Trp Leu 
    610                 615                 620                 


Arg Ser Ile Val Lys Asp Phe Ser Asp Glu Thr Gly Lys Ile Asp Val 
625                 630                 635                 640 


Ile Asp Lys Ser Ser Asp Thr Leu Ala Ile Val Pro Tyr Ile Gly Pro 
                645                 650                 655     


Leu Leu Asn Ile Gly Asn Asp Ile Arg His Gly Asp Phe Val Gly Ala 
            660                 665                 670         


Ile Glu Leu Ala Gly Ile Thr Ala Leu Leu Glu Tyr Val Pro Glu Phe 
        675                 680                 685             


Thr Ile Pro Ile Leu Val Gly Leu Glu Val Ile Gly Gly Glu Leu Ala 
    690                 695                 700                 


Arg Glu Gln Val Glu Ala Ile Val Asn Asn Ala Leu Asp Lys Arg Asp 
705                 710                 715                 720 


Gln Lys Trp Ala Glu Val Tyr Asn Ile Thr Lys Ala Gln Trp Trp Gly 
                725                 730                 735     


Thr Ile His Leu Gln Ile Asn Thr Arg Leu Ala His Thr Tyr Lys Ala 
            740                 745                 750         


Leu Ser Arg Gln Ala Asn Ala Ile Lys Met Asn Met Glu Phe Gln Leu 
        755                 760                 765             


Ala Asn Tyr Lys Gly Asn Ile Asp Asp Lys Ala Lys Ile Lys Asn Ala 
    770                 775                 780                 


Ile Ser Glu Thr Glu Ile Leu Leu Asn Lys Ser Val Glu Gln Ala Met 
785                 790                 795                 800 


Lys Asn Thr Glu Lys Phe Met Ile Lys Leu Ser Asn Ser Tyr Leu Thr 
                805                 810                 815     


Lys Glu Met Ile Pro Lys Val Gln Asp Asn Leu Lys Asn Phe Asp Leu 
            820                 825                 830         


Glu Thr Lys Lys Thr Leu Asp Lys Phe Ile Lys Glu Lys Glu Asp Ile 
        835                 840                 845             


Leu Gly Thr Asn Leu Ser Ser Ser Leu Arg Arg Lys Val Ser Ile Arg 
    850                 855                 860                 


Leu Asn Lys Asn Ile Ala Phe Asp Ile Asn Asp Ile Pro Phe Ser Glu 
865                 870                 875                 880 


Phe Asp Asp Leu Ile Asn Gln Tyr Lys Asn Glu Ile Glu Asp Tyr Glu 
                885                 890                 895     


Val Leu Asn Leu Gly Ala Glu Asp Gly Lys Ile Lys Asp Leu Ser Gly 
            900                 905                 910         


Thr Thr Ser Asp Ile Asn Ile Gly Ser Asp Ile Glu Leu Ala Asp Gly 
        915                 920                 925             


Arg Glu Asn Lys Ala Ile Lys Ile Lys Gly Ser Glu Asn Ser Thr Ile 
    930                 935                 940                 


Lys Ile Ala Met Asn Lys Tyr Leu Arg Phe Ser Ala Thr Asp Asn Phe 
945                 950                 955                 960 


Ser Ile Ser Phe Trp Ile Lys His Pro Lys Pro Thr Asn Leu Leu Asn 
                965                 970                 975     


Asn Gly Ile Glu Tyr Thr Leu Val Glu Asn Phe Asn Gln Arg Gly Trp 
            980                 985                 990         


Lys Ile Ser Ile Gln Asp Ser Lys  Leu Ile Trp Tyr Leu  Arg Asp His 
        995                 1000                 1005             


Asn Asn  Ser Ile Lys Ile Val  Thr Pro Asp Tyr Ile  Ala Phe Asn 
    1010                 1015                 1020             


Gly Trp  Asn Leu Ile Thr Ile  Thr Asn Asn Arg Ser  Lys Gly Ser 
    1025                 1030                 1035             


Ile Val  Tyr Val Asn Gly Ser  Lys Ile Glu Glu Lys  Asp Ile Ser 
    1040                 1045                 1050             


Ser Ile  Trp Asn Thr Glu Val  Asp Asp Pro Ile Ile  Phe Arg Leu 
    1055                 1060                 1065             


Lys Asn  Asn Arg Asp Thr Gln  Ala Phe Thr Leu Leu  Asp Gln Phe 
    1070                 1075                 1080             


Ser Ile  Tyr Arg Lys Glu Leu  Asn Gln Asn Glu Val  Val Lys Leu 
    1085                 1090                 1095             


Tyr Asn  Tyr Tyr Phe Asn Ser  Asn Tyr Ile Arg Asp  Ile Trp Gly 
    1100                 1105                 1110             


Asn Pro  Leu Gln Tyr Asn Lys  Lys Tyr Tyr Leu Gln  Thr Gln Asp 
    1115                 1120                 1125             


Lys Pro  Gly Lys Gly Leu Ile  Arg Glu Tyr Trp Ser  Ser Phe Gly 
    1130                 1135                 1140             


Tyr Asp  Tyr Val Ile Leu Ser  Asp Ser Lys Thr Ile  Thr Phe Pro 
    1145                 1150                 1155             


Asn Asn  Ile Arg Tyr Gly Ala  Leu Tyr Asn Gly Ser  Lys Val Leu 
    1160                 1165                 1170             


Ile Lys  Asn Ser Lys Lys Leu  Asp Gly Leu Val Arg  Asn Lys Asp 
    1175                 1180                 1185             


Phe Ile  Gln Leu Glu Ile Asp  Gly Tyr Asn Met Gly  Ile Ser Ala 
    1190                 1195                 1200             


Asp Arg  Phe Asn Glu Asp Thr  Asn Tyr Ile Gly Thr  Thr Tyr Gly 
    1205                 1210                 1215             


Thr Thr  His Asp Leu Thr Thr  Asp Phe Glu Ile Ile  Gln Arg Gln 
    1220                 1225                 1230             


Glu Lys  Tyr Arg Asn Tyr Cys  Gln Leu Lys Thr Pro  Tyr Asn Ile 
    1235                 1240                 1245             


Phe His  Lys Ser Gly Leu Met  Ser Thr Glu Thr Ser  Lys Pro Thr 
    1250                 1255                 1260             


Phe His  Asp Tyr Arg Asp Trp  Val Tyr Ser Ser Ala  Trp Tyr Phe 
    1265                 1270                 1275             


Gln Asn  Tyr Glu Asn Leu Asn  Leu Arg Lys His Thr  Lys Thr Asn 
    1280                 1285                 1290             


Trp Tyr  Phe Ile Pro Lys Asp  Glu Gly Trp Asp Glu  Asp Leu Glu 
    1295                 1300                 1305             


Val Leu  Phe Gln Gly Pro His  His His His His His  His His His 
    1310                 1315                 1320             


His 
    


<210>  35
<211>  1296
<212>  PRT
<213>  Clostridium botulinum

<400>  35

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Val Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  36
<211>  1291
<212>  PRT
<213>  Clostridium botulinum

<400>  36

Met Pro Val Thr Ile Asn Asn Phe Asn Tyr Asn Asp Pro Ile Asp Asn 
1               5                   10                  15      


Asn Asn Ile Ile Met Met Glu Pro Pro Phe Ala Arg Gly Thr Gly Arg 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Tyr Thr Phe Gly Tyr Lys Pro Glu Asp Phe Asn Lys Ser Ser Gly 
    50                  55                  60                  


Ile Phe Asn Arg Asp Val Cys Glu Tyr Tyr Asp Pro Asp Tyr Leu Asn 
65                  70                  75                  80  


Thr Asn Asp Lys Lys Asn Ile Phe Leu Gln Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Lys Ser Lys Pro Leu Gly Glu Lys Leu Leu Glu Met Ile 
            100                 105                 110         


Ile Asn Gly Ile Pro Tyr Leu Gly Asp Arg Arg Val Pro Leu Glu Glu 
        115                 120                 125             


Phe Asn Thr Asn Ile Ala Ser Val Thr Val Asn Lys Leu Ile Ser Asn 
    130                 135                 140                 


Pro Gly Glu Val Glu Arg Lys Lys Gly Ile Phe Ala Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Asn Glu Asn Glu Thr Ile Asp Ile Gly 
                165                 170                 175     


Ile Gln Asn His Phe Ala Ser Arg Glu Gly Phe Gly Gly Ile Met Gln 
            180                 185                 190         


Met Lys Phe Cys Pro Glu Tyr Val Ser Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Gly Ala Ser Ile Phe Asn Arg Arg Gly Tyr Phe Ser Asp Pro 
    210                 215                 220                 


Ala Leu Ile Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Val Asp Asp Leu Pro Ile Val Pro Asn Glu Lys Lys Phe 
                245                 250                 255     


Phe Met Gln Ser Thr Asp Ala Ile Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly Gln Asp Pro Ser Ile Ile Thr Pro Ser Thr Asp Lys Ser Ile 
        275                 280                 285             


Tyr Asp Lys Val Leu Gln Asn Phe Arg Gly Ile Val Asp Arg Leu Asn 
    290                 295                 300                 


Lys Val Leu Val Cys Ile Ser Asp Pro Asn Ile Asn Ile Asn Ile Tyr 
305                 310                 315                 320 


Lys Asn Lys Phe Lys Asp Lys Tyr Lys Phe Val Glu Asp Ser Glu Gly 
                325                 330                 335     


Lys Tyr Ser Ile Asp Val Glu Ser Phe Asp Lys Leu Tyr Lys Ser Leu 
            340                 345                 350         


Met Phe Gly Phe Thr Glu Thr Asn Ile Ala Glu Asn Tyr Lys Ile Lys 
        355                 360                 365             


Thr Arg Ala Ser Tyr Phe Ser Asp Ser Leu Pro Pro Val Lys Ile Lys 
    370                 375                 380                 


Asn Leu Leu Asp Asn Glu Ile Tyr Thr Ile Glu Glu Gly Phe Asn Ile 
385                 390                 395                 400 


Ser Asp Lys Asp Met Glu Lys Glu Tyr Arg Gly Gln Asn Lys Ala Ile 
                405                 410                 415     


Asn Lys Gln Ala Tyr Glu Glu Ile Ser Lys Glu His Leu Ala Val Tyr 
            420                 425                 430         


Lys Ile Gln Met Cys Lys Ser Val Lys Ala Pro Gly Ile Cys Ile Asp 
        435                 440                 445             


Val Asp Asn Glu Asp Leu Phe Phe Ile Ala Asp Lys Asn Ser Phe Ser 
    450                 455                 460                 


Asp Asp Leu Ser Lys Asn Glu Arg Ile Glu Tyr Asn Thr Gln Ser Asn 
465                 470                 475                 480 


Tyr Ile Glu Asn Asp Phe Pro Ile Asn Glu Leu Ile Leu Asp Thr Asp 
                485                 490                 495     


Leu Ile Ser Lys Ile Glu Leu Pro Ser Glu Asn Thr Glu Ser Leu Thr 
            500                 505                 510         


Asp Phe Asn Val Asp Val Pro Val Tyr Glu Lys Gln Pro Ala Ile Lys 
        515                 520                 525             


Lys Ile Phe Thr Asp Glu Asn Thr Ile Phe Gln Tyr Leu Tyr Ser Gln 
    530                 535                 540                 


Thr Phe Pro Leu Asp Ile Arg Asp Ile Ser Leu Thr Ser Ser Phe Asp 
545                 550                 555                 560 


Asp Ala Leu Leu Phe Ser Asn Lys Val Tyr Ser Phe Phe Ser Met Asp 
                565                 570                 575     


Tyr Ile Lys Thr Ala Asn Lys Val Val Glu Ala Gly Leu Phe Ala Gly 
            580                 585                 590         


Trp Val Lys Gln Ile Val Asn Asp Phe Val Ile Glu Ala Asn Lys Ser 
        595                 600                 605             


Asn Thr Met Asp Lys Ile Ala Asp Ile Ser Leu Ile Val Pro Tyr Ile 
    610                 615                 620                 


Gly Leu Ala Leu Asn Val Gly Asn Glu Thr Ala Lys Gly Asn Phe Glu 
625                 630                 635                 640 


Asn Ala Phe Glu Ile Ala Gly Ala Ser Ile Leu Leu Glu Phe Ile Pro 
                645                 650                 655     


Glu Leu Leu Ile Pro Val Val Gly Ala Phe Leu Leu Glu Ser Tyr Ile 
            660                 665                 670         


Asp Asn Lys Asn Lys Ile Ile Lys Thr Ile Asp Asn Ala Leu Thr Lys 
        675                 680                 685             


Arg Asn Glu Lys Trp Ser Asp Met Tyr Gly Leu Ile Val Ala Gln Trp 
    690                 695                 700                 


Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile Lys Glu Gly Met Tyr 
705                 710                 715                 720 


Lys Ala Leu Asn Tyr Gln Ala Gln Ala Leu Glu Glu Ile Ile Lys Tyr 
                725                 730                 735     


Arg Tyr Asn Ile Tyr Ser Glu Lys Glu Lys Ser Asn Ile Asn Ile Asp 
            740                 745                 750         


Phe Asn Asp Ile Asn Ser Lys Leu Asn Glu Gly Ile Asn Gln Ala Ile 
        755                 760                 765             


Asp Asn Ile Asn Asn Phe Ile Asn Gly Cys Ser Val Ser Tyr Leu Met 
    770                 775                 780                 


Lys Lys Met Ile Pro Leu Ala Val Glu Lys Leu Leu Asp Phe Asp Asn 
785                 790                 795                 800 


Thr Leu Lys Lys Asn Leu Leu Asn Tyr Ile Asp Glu Asn Lys Leu Tyr 
                805                 810                 815     


Leu Ile Gly Ser Ala Glu Tyr Glu Lys Ser Lys Val Asn Lys Tyr Leu 
            820                 825                 830         


Lys Thr Ile Met Pro Phe Asp Leu Ser Ile Tyr Thr Asn Asp Thr Ile 
        835                 840                 845             


Leu Ile Glu Met Phe Asn Lys Tyr Asn Ser Glu Ile Leu Asn Asn Ile 
    850                 855                 860                 


Ile Leu Asn Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly 
865                 870                 875                 880 


Tyr Gly Ala Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys 
                885                 890                 895     


Asn Gln Phe Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr 
            900                 905                 910         


Gln Asn Gln Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val 
        915                 920                 925             


Ser Phe Trp Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn 
    930                 935                 940                 


Tyr Ile His Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser 
945                 950                 955                 960 


Gly Trp Lys Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile 
                965                 970                 975     


Asp Ile Asn Gly Lys Thr Lys Ser Val Phe Phe Glu Tyr Asn Ile Arg 
            980                 985                 990         


Glu Asp Ile Ser Glu Tyr Ile Asn  Arg Trp Phe Phe Val  Thr Ile Thr 
        995                 1000                 1005             


Asn Asn  Leu Asn Asn Ala Lys  Ile Tyr Ile Asn Gly  Lys Leu Glu 
    1010                 1015                 1020             


Ser Asn  Thr Asp Ile Lys Asp  Ile Arg Glu Val Ile  Ala Asn Gly 
    1025                 1030                 1035             


Glu Ile  Ile Phe Lys Leu Asp  Gly Asp Ile Asp Arg  Thr Gln Phe 
    1040                 1045                 1050             


Ile Trp  Met Lys Tyr Phe Ser  Ile Phe Asn Thr Glu  Leu Ser Gln 
    1055                 1060                 1065             


Ser Asn  Ile Glu Glu Arg Tyr  Lys Ile Gln Ser Tyr  Ser Glu Tyr 
    1070                 1075                 1080             


Leu Lys  Asp Phe Trp Gly Asn  Pro Leu Met Tyr Asn  Lys Glu Tyr 
    1085                 1090                 1095             


Tyr Met  Phe Asn Ala Gly Asn  Lys Asn Ser Tyr Ile  Lys Leu Lys 
    1100                 1105                 1110             


Lys Asp  Ser Pro Val Gly Glu  Ile Leu Thr Arg Ser  Lys Tyr Asn 
    1115                 1120                 1125             


Gln Asn  Ser Lys Tyr Ile Asn  Tyr Arg Asp Leu Tyr  Ile Gly Glu 
    1130                 1135                 1140             


Lys Phe  Ile Ile Arg Arg Lys  Ser Asn Ser Gln Ser  Ile Asn Asp 
    1145                 1150                 1155             


Asp Ile  Val Arg Lys Glu Asp  Tyr Ile Tyr Leu Asp  Phe Phe Asn 
    1160                 1165                 1170             


Leu Asn  Gln Glu Trp Arg Val  Tyr Thr Tyr Lys Tyr  Phe Lys Lys 
    1175                 1180                 1185             


Glu Glu  Glu Lys Leu Phe Leu  Ala Pro Ile Ser Asp  Ser Asp Glu 
    1190                 1195                 1200             


Phe Tyr  Asn Thr Ile Gln Ile  Lys Glu Tyr Asp Glu  Gln Pro Thr 
    1205                 1210                 1215             


Tyr Ser  Cys Gln Leu Leu Phe  Lys Lys Asp Glu Glu  Ser Thr Asp 
    1220                 1225                 1230             


Glu Ile  Gly Leu Ile Gly Ile  His Arg Phe Tyr Glu  Ser Gly Ile 
    1235                 1240                 1245             


Val Phe  Glu Glu Tyr Lys Asp  Tyr Phe Cys Ile Ser  Lys Trp Tyr 
    1250                 1255                 1260             


Leu Lys  Glu Val Lys Arg Lys  Pro Tyr Asn Leu Lys  Leu Gly Cys 
    1265                 1270                 1275             


Asn Trp  Gln Phe Ile Pro Lys  Asp Glu Gly Trp Thr  Glu 
    1280                 1285                 1290     


<210>  37
<211>  1291
<212>  PRT
<213>  Clostridium botulinum

<400>  37

Met Pro Ile Thr Ile Asn Asn Phe Asn Tyr Ser Asp Pro Val Asp Asn 
1               5                   10                  15      


Lys Asn Ile Leu Tyr Leu Asp Thr His Leu Asn Thr Leu Ala Asn Glu 
            20                  25                  30          


Pro Glu Lys Ala Phe Arg Ile Thr Gly Asn Ile Trp Val Ile Pro Asp 
        35                  40                  45              


Arg Phe Ser Arg Asn Ser Asn Pro Asn Leu Asn Lys Pro Pro Arg Val 
    50                  55                  60                  


Thr Ser Pro Lys Ser Gly Tyr Tyr Asp Pro Asn Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Ser Asp Lys Asp Pro Phe Leu Lys Glu Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Ser Arg Glu Ile Gly Glu Glu Leu Ile Tyr Arg Leu Ser Thr 
            100                 105                 110         


Asp Ile Pro Phe Pro Gly Asn Asn Asn Thr Pro Ile Asn Thr Phe Asp 
        115                 120                 125             


Phe Asp Val Asp Phe Asn Ser Val Asp Val Lys Thr Arg Gln Gly Asn 
    130                 135                 140                 


Asn Trp Val Lys Thr Gly Ser Ile Asn Pro Ser Val Ile Ile Thr Gly 
145                 150                 155                 160 


Pro Arg Glu Asn Ile Ile Asp Pro Glu Thr Ser Thr Phe Lys Leu Thr 
                165                 170                 175     


Asn Asn Thr Phe Ala Ala Gln Glu Gly Phe Gly Ala Leu Ser Ile Ile 
            180                 185                 190         


Ser Ile Ser Pro Arg Phe Met Leu Thr Tyr Ser Asn Ala Thr Asn Asp 
        195                 200                 205             


Val Gly Glu Gly Arg Phe Ser Lys Ser Glu Phe Cys Met Asp Pro Ile 
    210                 215                 220                 


Leu Ile Leu Met His Glu Leu Asn His Ala Met His Asn Leu Tyr Gly 
225                 230                 235                 240 


Ile Ala Ile Pro Asn Asp Gln Thr Ile Ser Ser Val Thr Ser Asn Ile 
                245                 250                 255     


Phe Tyr Ser Gln Tyr Asn Val Lys Leu Glu Tyr Ala Glu Ile Tyr Ala 
            260                 265                 270         


Phe Gly Gly Pro Thr Ile Asp Leu Ile Pro Lys Ser Ala Arg Lys Tyr 
        275                 280                 285             


Phe Glu Glu Lys Ala Leu Asp Tyr Tyr Arg Ser Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Ser Ile Thr Thr Ala Asn Pro Ser Ser Phe Asn Lys Tyr Ile Gly 
305                 310                 315                 320 


Glu Tyr Lys Gln Lys Leu Ile Arg Lys Tyr Arg Phe Val Val Glu Ser 
                325                 330                 335     


Ser Gly Glu Val Thr Val Asn Arg Asn Lys Phe Val Glu Leu Tyr Asn 
            340                 345                 350         


Glu Leu Thr Gln Ile Phe Thr Glu Phe Asn Tyr Ala Lys Ile Tyr Asn 
        355                 360                 365             


Val Gln Asn Arg Lys Ile Tyr Leu Ser Asn Val Tyr Thr Pro Val Thr 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Val Tyr Asp Ile Gln Asn Gly Phe Asn 
385                 390                 395                 400 


Ile Pro Lys Ser Asn Leu Asn Val Leu Phe Met Gly Gln Asn Leu Ser 
                405                 410                 415     


Arg Asn Pro Ala Leu Arg Lys Val Asn Pro Glu Asn Met Leu Tyr Leu 
            420                 425                 430         


Phe Thr Lys Phe Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn 
        435                 440                 445             


Lys Thr Leu Asp Cys Arg Glu Leu Leu Val Lys Asn Thr Asp Leu Pro 
    450                 455                 460                 


Phe Ile Gly Asp Ile Ser Asp Val Lys Thr Asp Ile Phe Leu Arg Lys 
465                 470                 475                 480 


Asp Ile Asn Glu Glu Thr Glu Val Ile Tyr Tyr Pro Asp Asn Val Ser 
                485                 490                 495     


Val Asp Gln Val Ile Leu Ser Lys Asn Thr Ser Glu His Gly Gln Leu 
            500                 505                 510         


Asp Leu Leu Tyr Pro Ser Ile Asp Ser Glu Ser Glu Ile Leu Pro Gly 
        515                 520                 525             


Glu Asn Gln Val Phe Tyr Asp Asn Arg Thr Gln Asn Val Asp Tyr Leu 
    530                 535                 540                 


Asn Ser Tyr Tyr Tyr Leu Glu Ser Gln Lys Leu Ser Asp Asn Val Glu 
545                 550                 555                 560 


Asp Phe Thr Phe Thr Arg Ser Ile Glu Glu Ala Leu Asp Asn Ser Ala 
                565                 570                 575     


Lys Val Tyr Thr Tyr Phe Pro Thr Leu Ala Asn Lys Val Asn Ala Gly 
            580                 585                 590         


Val Gln Gly Gly Leu Phe Leu Met Trp Ala Asn Asp Val Val Glu Asp 
        595                 600                 605             


Phe Thr Thr Asn Ile Leu Arg Lys Asp Thr Leu Asp Lys Ile Ser Asp 
    610                 615                 620                 


Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Ser Asn 
625                 630                 635                 640 


Ser Val Arg Arg Gly Asn Phe Thr Glu Ala Phe Ala Val Thr Gly Val 
                645                 650                 655     


Thr Ile Leu Leu Glu Ala Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly 
            660                 665                 670         


Ala Phe Val Ile Tyr Ser Lys Val Gln Glu Arg Asn Glu Ile Ile Lys 
        675                 680                 685             


Thr Ile Asp Asn Cys Leu Glu Gln Arg Ile Lys Arg Trp Lys Asp Ser 
    690                 695                 700                 


Tyr Glu Trp Met Met Gly Thr Trp Leu Ser Arg Ile Ile Thr Gln Phe 
705                 710                 715                 720 


Asn Asn Ile Ser Tyr Gln Met Tyr Asp Ser Leu Asn Tyr Gln Ala Gly 
                725                 730                 735     


Ala Ile Lys Ala Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser 
            740                 745                 750         


Asp Lys Glu Asn Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu 
        755                 760                 765             


Asp Val Lys Ile Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg 
    770                 775                 780                 


Glu Cys Ser Val Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile 
785                 790                 795                 800 


Asp Glu Leu Asn Glu Phe Asp Arg Asn Thr Lys Ala Lys Leu Ile Asn 
                805                 810                 815     


Leu Ile Asp Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Lys Leu 
            820                 825                 830         


Lys Ala Lys Val Asn Asn Ser Phe Gln Asn Thr Ile Pro Phe Asn Ile 
        835                 840                 845             


Phe Ser Tyr Thr Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr 
    850                 855                 860                 


Phe Asn Asn Ile Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Arg Lys 
865                 870                 875                 880 


Asn Thr Leu Val Asp Thr Ser Gly Tyr Asn Ala Glu Val Ser Glu Glu 
                885                 890                 895     


Gly Asp Val Gln Leu Asn Pro Ile Phe Pro Phe Asp Phe Lys Leu Gly 
            900                 905                 910         


Ser Ser Gly Glu Asp Arg Gly Lys Val Ile Val Thr Gln Asn Glu Asn 
        915                 920                 925             


Ile Val Tyr Asn Ser Met Tyr Glu Ser Phe Ser Ile Ser Phe Trp Ile 
    930                 935                 940                 


Arg Ile Asn Lys Trp Val Ser Asn Leu Pro Gly Tyr Thr Ile Ile Asp 
945                 950                 955                 960 


Ser Val Lys Asn Asn Ser Gly Trp Ser Ile Gly Ile Ile Ser Asn Phe 
                965                 970                 975     


Leu Val Phe Thr Leu Lys Gln Asn Glu Asp Ser Glu Gln Ser Ile Asn 
            980                 985                 990         


Phe Ser Tyr Asp Ile Ser Asn Asn  Ala Pro Gly Tyr Asn  Lys Trp Phe 
        995                 1000                 1005             


Phe Val  Thr Val Thr Asn Asn  Met Met Gly Asn Met  Lys Ile Tyr 
    1010                 1015                 1020             


Ile Asn  Gly Lys Leu Ile Asp  Thr Ile Lys Val Lys  Glu Leu Thr 
    1025                 1030                 1035             


Gly Ile  Asn Phe Ser Lys Thr  Ile Thr Phe Glu Ile  Asn Lys Ile 
    1040                 1045                 1050             


Pro Asp  Thr Gly Leu Ile Thr  Ser Asp Ser Asp Asn  Ile Asn Met 
    1055                 1060                 1065             


Trp Ile  Arg Asp Phe Tyr Ile  Phe Ala Lys Glu Leu  Asp Gly Lys 
    1070                 1075                 1080             


Asp Ile  Asn Ile Leu Phe Asn  Ser Leu Gln Tyr Thr  Asn Val Val 
    1085                 1090                 1095             


Lys Asp  Tyr Trp Gly Asn Asp  Leu Arg Tyr Asn Lys  Glu Tyr Tyr 
    1100                 1105                 1110             


Met Val  Asn Ile Asp Tyr Leu  Asn Arg Tyr Met Tyr  Ala Asn Ser 
    1115                 1120                 1125             


Arg Gln  Ile Val Phe Asn Thr  Arg Arg Asn Asn Asn  Asp Phe Asn 
    1130                 1135                 1140             


Glu Gly  Tyr Lys Ile Ile Ile  Lys Arg Ile Arg Gly  Asn Thr Asn 
    1145                 1150                 1155             


Asp Thr  Arg Val Arg Gly Gly  Asp Ile Leu Tyr Phe  Asp Met Thr 
    1160                 1165                 1170             


Ile Asn  Asn Lys Ala Tyr Asn  Leu Phe Met Lys Asn  Glu Thr Met 
    1175                 1180                 1185             


Tyr Ala  Asp Asn His Ser Thr  Glu Asp Ile Tyr Ala  Ile Gly Leu 
    1190                 1195                 1200             


Arg Glu  Gln Thr Lys Asp Ile  Asn Asp Asn Ile Ile  Phe Gln Ile 
    1205                 1210                 1215             


Gln Pro  Met Asn Asn Thr Tyr  Tyr Tyr Ala Ser Gln  Ile Phe Lys 
    1220                 1225                 1230             


Ser Asn  Phe Asn Gly Glu Asn  Ile Ser Gly Ile Cys  Ser Ile Gly 
    1235                 1240                 1245             


Thr Tyr  Arg Phe Arg Leu Gly  Gly Asp Trp Tyr Arg  His Asn Tyr 
    1250                 1255                 1260             


Leu Val  Pro Thr Val Lys Gln  Gly Asn Tyr Ala Ser  Leu Leu Glu 
    1265                 1270                 1275             


Ser Thr  Ser Thr His Trp Gly  Phe Val Pro Val Ser  Glu 
    1280                 1285                 1290     


<210>  38
<211>  1276
<212>  PRT
<213>  Clostridium botulinum

<400>  38

Met Thr Trp Pro Val Lys Asp Phe Asn Tyr Ser Asp Pro Val Asn Asp 
1               5                   10                  15      


Asn Asp Ile Leu Tyr Leu Arg Ile Pro Gln Asn Lys Leu Ile Thr Thr 
            20                  25                  30          


Pro Val Lys Ala Phe Met Ile Thr Gln Asn Ile Trp Val Ile Pro Glu 
        35                  40                  45              


Arg Phe Ser Ser Asp Thr Asn Pro Ser Leu Ser Lys Pro Pro Arg Pro 
    50                  55                  60                  


Thr Ser Lys Tyr Gln Ser Tyr Tyr Asp Pro Ser Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Glu Gln Lys Asp Thr Phe Leu Lys Gly Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Glu Arg Asp Ile Gly Lys Lys Leu Ile Asn Tyr Leu Val Val 
            100                 105                 110         


Gly Ser Pro Phe Met Gly Asp Ser Ser Thr Pro Glu Asp Thr Phe Asp 
        115                 120                 125             


Phe Thr Arg His Thr Thr Asn Ile Ala Val Glu Lys Phe Glu Asn Gly 
    130                 135                 140                 


Ser Trp Lys Val Thr Asn Ile Ile Thr Pro Ser Val Leu Ile Phe Gly 
145                 150                 155                 160 


Pro Leu Pro Asn Ile Leu Asp Tyr Thr Ala Ser Leu Thr Leu Gln Gly 
                165                 170                 175     


Gln Gln Ser Asn Pro Ser Phe Glu Gly Phe Gly Thr Leu Ser Ile Leu 
            180                 185                 190         


Lys Val Ala Pro Glu Phe Leu Leu Thr Phe Ser Asp Val Thr Ser Asn 
        195                 200                 205             


Gln Ser Ser Ala Val Leu Gly Lys Ser Ile Phe Cys Met Asp Pro Val 
    210                 215                 220                 


Ile Ala Leu Met His Glu Leu Thr His Ser Leu His Gln Leu Tyr Gly 
225                 230                 235                 240 


Ile Asn Ile Pro Ser Asp Lys Arg Ile Arg Pro Gln Val Ser Glu Gly 
                245                 250                 255     


Phe Phe Ser Gln Asp Gly Pro Asn Val Gln Phe Glu Glu Leu Tyr Thr 
            260                 265                 270         


Phe Gly Gly Leu Asp Val Glu Ile Ile Pro Gln Ile Glu Arg Ser Gln 
        275                 280                 285             


Leu Arg Glu Lys Ala Leu Gly His Tyr Lys Asp Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Asn Ile Asn Lys Thr Ile Pro Ser Ser Trp Ile Ser Asn Ile Asp 
305                 310                 315                 320 


Lys Tyr Lys Lys Ile Phe Ser Glu Lys Tyr Asn Phe Asp Lys Asp Asn 
                325                 330                 335     


Thr Gly Asn Phe Val Val Asn Ile Asp Lys Phe Asn Ser Leu Tyr Ser 
            340                 345                 350         


Asp Leu Thr Asn Val Met Ser Glu Val Val Tyr Ser Ser Gln Tyr Asn 
        355                 360                 365             


Val Lys Asn Arg Thr His Tyr Phe Ser Arg His Tyr Leu Pro Val Phe 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Ile Tyr Thr Ile Arg Asp Gly Phe Asn 
385                 390                 395                 400 


Leu Thr Asn Lys Gly Phe Asn Ile Glu Asn Ser Gly Gln Asn Ile Glu 
                405                 410                 415     


Arg Asn Pro Ala Leu Gln Lys Leu Ser Ser Glu Ser Val Val Asp Leu 
            420                 425                 430         


Phe Thr Lys Val Cys Leu Arg Leu Thr Lys Asn Ser Arg Asp Asp Ser 
        435                 440                 445             


Thr Cys Ile Lys Val Lys Asn Asn Arg Leu Pro Tyr Val Ala Asp Lys 
    450                 455                 460                 


Asp Ser Ile Ser Gln Glu Ile Phe Glu Asn Lys Ile Ile Thr Asp Glu 
465                 470                 475                 480 


Thr Asn Val Gln Asn Tyr Ser Asp Lys Phe Ser Leu Asp Glu Ser Ile 
                485                 490                 495     


Leu Asp Gly Gln Val Pro Ile Asn Pro Glu Ile Val Asp Pro Leu Leu 
            500                 505                 510         


Pro Asn Val Asn Met Glu Pro Leu Asn Leu Pro Gly Glu Glu Ile Val 
        515                 520                 525             


Phe Tyr Asp Asp Ile Thr Lys Tyr Val Asp Tyr Leu Asn Ser Tyr Tyr 
    530                 535                 540                 


Tyr Leu Glu Ser Gln Lys Leu Ser Asn Asn Val Glu Asn Ile Thr Leu 
545                 550                 555                 560 


Thr Thr Ser Val Glu Glu Ala Leu Gly Tyr Ser Asn Lys Ile Tyr Thr 
                565                 570                 575     


Phe Leu Pro Ser Leu Ala Glu Lys Val Asn Lys Gly Val Gln Ala Gly 
            580                 585                 590         


Leu Phe Leu Asn Trp Ala Asn Glu Val Val Glu Asp Phe Thr Thr Asn 
        595                 600                 605             


Ile Met Lys Lys Asp Thr Leu Asp Lys Ile Ser Asp Val Ser Val Ile 
    610                 615                 620                 


Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Ser Ala Leu Arg 
625                 630                 635                 640 


Gly Asn Phe Asn Gln Ala Phe Ala Thr Ala Gly Val Ala Phe Leu Leu 
                645                 650                 655     


Glu Gly Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly Val Phe Thr Phe 
            660                 665                 670         


Tyr Ser Ser Ile Gln Glu Arg Glu Lys Ile Ile Lys Thr Ile Glu Asn 
        675                 680                 685             


Cys Leu Glu Gln Arg Val Lys Arg Trp Lys Asp Ser Tyr Gln Trp Met 
    690                 695                 700                 


Val Ser Asn Trp Leu Ser Arg Ile Thr Thr Gln Phe Asn His Ile Asn 
705                 710                 715                 720 


Tyr Gln Met Tyr Asp Ser Leu Ser Tyr Gln Ala Asp Ala Ile Lys Ala 
                725                 730                 735     


Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser Asp Lys Glu Asn 
            740                 745                 750         


Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu Asp Val Lys Ile 
        755                 760                 765             


Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg Glu Cys Ser Val 
    770                 775                 780                 


Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile Asp Glu Leu Asn 
785                 790                 795                 800 


Lys Phe Asp Leu Arg Thr Lys Thr Glu Leu Ile Asn Leu Ile Asp Ser 
                805                 810                 815     


His Asn Ile Ile Leu Val Gly Glu Val Asp Arg Leu Lys Ala Lys Val 
            820                 825                 830         


Asn Glu Ser Phe Glu Asn Thr Met Pro Phe Asn Ile Phe Ser Tyr Thr 
        835                 840                 845             


Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr Phe Asn Ser Ile 
    850                 855                 860                 


Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Lys Lys Asn Ala Leu Val 
865                 870                 875                 880 


Asp Thr Ser Gly Tyr Asn Ala Glu Val Arg Val Gly Asp Asn Val Gln 
                885                 890                 895     


Leu Asn Thr Ile Tyr Thr Asn Asp Phe Lys Leu Ser Ser Ser Gly Asp 
            900                 905                 910         


Lys Ile Ile Val Asn Leu Asn Asn Asn Ile Leu Tyr Ser Ala Ile Tyr 
        915                 920                 925             


Glu Asn Ser Ser Val Ser Phe Trp Ile Lys Ile Ser Lys Asp Leu Thr 
    930                 935                 940                 


Asn Ser His Asn Glu Tyr Thr Ile Ile Asn Ser Ile Glu Gln Asn Ser 
945                 950                 955                 960 


Gly Trp Lys Leu Cys Ile Arg Asn Gly Asn Ile Glu Trp Ile Leu Gln 
                965                 970                 975     


Asp Val Asn Arg Lys Tyr Lys Ser Leu Ile Phe Asp Tyr Ser Glu Ser 
            980                 985                 990         


Leu Ser His Thr Gly Tyr Thr Asn  Lys Trp Phe Phe Val  Thr Ile Thr 
        995                 1000                 1005             


Asn Asn  Ile Met Gly Tyr Met  Lys Leu Tyr Ile Asn  Gly Glu Leu 
    1010                 1015                 1020             


Lys Gln  Ser Gln Lys Ile Glu  Asp Leu Asp Glu Val  Lys Leu Asp 
    1025                 1030                 1035             


Lys Thr  Ile Val Phe Gly Ile  Asp Glu Asn Ile Asp  Glu Asn Gln 
    1040                 1045                 1050             


Met Leu  Trp Ile Arg Asp Phe  Asn Ile Phe Ser Lys  Glu Leu Ser 
    1055                 1060                 1065             


Asn Glu  Asp Ile Asn Ile Val  Tyr Glu Gly Gln Ile  Leu Arg Asn 
    1070                 1075                 1080             


Val Ile  Lys Asp Tyr Trp Gly  Asn Pro Leu Lys Phe  Asp Thr Glu 
    1085                 1090                 1095             


Tyr Tyr  Ile Ile Asn Asp Asn  Tyr Ile Asp Arg Tyr  Ile Ala Pro 
    1100                 1105                 1110             


Glu Ser  Asn Val Leu Val Leu  Val Gln Tyr Pro Asp  Arg Ser Lys 
    1115                 1120                 1125             


Leu Tyr  Thr Gly Asn Pro Ile  Thr Ile Lys Ser Val  Ser Asp Lys 
    1130                 1135                 1140             


Asn Pro  Tyr Ser Arg Ile Leu  Asn Gly Asp Asn Ile  Ile Leu His 
    1145                 1150                 1155             


Met Leu  Tyr Asn Ser Arg Lys  Tyr Met Ile Ile Arg  Asp Thr Asp 
    1160                 1165                 1170             


Thr Ile  Tyr Ala Thr Gln Gly  Gly Glu Cys Ser Gln  Asn Cys Val 
    1175                 1180                 1185             


Tyr Ala  Leu Lys Leu Gln Ser  Asn Leu Gly Asn Tyr  Gly Ile Gly 
    1190                 1195                 1200             


Ile Phe  Ser Ile Lys Asn Ile  Val Ser Lys Asn Lys  Tyr Cys Ser 
    1205                 1210                 1215             


Gln Ile  Phe Ser Ser Phe Arg  Glu Asn Thr Met Leu  Leu Ala Asp 
    1220                 1225                 1230             


Ile Tyr  Lys Pro Trp Arg Phe  Ser Phe Lys Asn Ala  Tyr Thr Pro 
    1235                 1240                 1245             


Val Ala  Val Thr Asn Tyr Glu  Thr Lys Leu Leu Ser  Thr Ser Ser 
    1250                 1255                 1260             


Phe Trp  Lys Phe Ile Ser Arg  Asp Pro Gly Trp Val  Glu 
    1265                 1270                 1275     


<210>  39
<211>  1251
<212>  PRT
<213>  Clostridium botulinum

<400>  39

Met Pro Lys Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp Arg 
1               5                   10                  15      


Thr Ile Leu Tyr Ile Lys Pro Gly Gly Cys Gln Glu Phe Tyr Lys Ser 
            20                  25                  30          


Phe Asn Ile Met Lys Asn Ile Trp Ile Ile Pro Glu Arg Asn Val Ile 
        35                  40                  45              


Gly Thr Thr Pro Gln Asp Phe His Pro Pro Thr Ser Leu Lys Asn Gly 
    50                  55                  60                  


Asp Ser Ser Tyr Tyr Asp Pro Asn Tyr Leu Gln Ser Asp Glu Glu Lys 
65                  70                  75                  80  


Asp Arg Phe Leu Lys Ile Val Thr Lys Ile Phe Asn Arg Ile Asn Asn 
                85                  90                  95      


Asn Leu Ser Gly Gly Ile Leu Leu Glu Glu Leu Ser Lys Ala Asn Pro 
            100                 105                 110         


Tyr Leu Gly Asn Asp Asn Thr Pro Asp Asn Gln Phe His Ile Gly Asp 
        115                 120                 125             


Ala Ser Ala Val Glu Ile Lys Phe Ser Asn Gly Ser Gln Asp Ile Leu 
    130                 135                 140                 


Leu Pro Asn Val Ile Ile Met Gly Ala Glu Pro Asp Leu Phe Glu Thr 
145                 150                 155                 160 


Asn Ser Ser Asn Ile Ser Leu Arg Asn Asn Tyr Met Pro Ser Asn His 
                165                 170                 175     


Arg Phe Gly Ser Ile Ala Ile Val Thr Phe Ser Pro Glu Tyr Ser Phe 
            180                 185                 190         


Arg Phe Asn Asp Asn Cys Met Asn Glu Phe Ile Gln Asp Pro Ala Leu 
        195                 200                 205             


Thr Leu Met His Glu Leu Ile His Ser Leu His Gly Leu Tyr Gly Ala 
    210                 215                 220                 


Lys Gly Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn Pro Leu 
225                 230                 235                 240 


Ile Thr Asn Ile Arg Gly Thr Asn Ile Glu Glu Phe Leu Thr Phe Gly 
                245                 250                 255     


Gly Thr Asp Leu Asn Ile Ile Thr Ser Ala Gln Ser Asn Asp Ile Tyr 
            260                 265                 270         


Thr Asn Leu Leu Ala Asp Tyr Lys Lys Ile Ala Ser Lys Leu Ser Lys 
        275                 280                 285             


Val Gln Val Ser Asn Pro Leu Leu Asn Pro Tyr Lys Asp Val Phe Glu 
    290                 295                 300                 


Ala Lys Tyr Gly Leu Asp Lys Asp Ala Ser Gly Ile Tyr Ser Val Asn 
305                 310                 315                 320 


Ile Asn Lys Phe Asn Asp Ile Phe Lys Lys Leu Tyr Ser Phe Thr Glu 
                325                 330                 335     


Phe Asp Leu Arg Thr Lys Phe Gln Val Lys Cys Arg Gln Thr Tyr Ile 
            340                 345                 350         


Gly Gln Tyr Lys Tyr Phe Lys Leu Ser Asn Leu Leu Asn Asp Ser Ile 
        355                 360                 365             


Tyr Asn Ile Ser Glu Gly Tyr Asn Ile Asn Asn Leu Lys Val Asn Phe 
    370                 375                 380                 


Arg Gly Gln Asn Ala Asn Leu Asn Pro Arg Ile Ile Thr Pro Ile Thr 
385                 390                 395                 400 


Gly Arg Gly Leu Val Lys Lys Ile Ile Arg Phe Cys Lys Asn Ile Val 
                405                 410                 415     


Ser Val Lys Gly Ile Arg Lys Ser Ile Cys Ile Glu Ile Asn Asn Gly 
            420                 425                 430         


Glu Leu Phe Phe Val Ala Ser Glu Asn Ser Tyr Asn Asp Asp Asn Ile 
        435                 440                 445             


Asn Thr Pro Lys Glu Ile Asp Asp Thr Val Thr Ser Asn Asn Asn Tyr 
    450                 455                 460                 


Glu Asn Asp Leu Asp Gln Val Ile Leu Asn Phe Asn Ser Glu Ser Ala 
465                 470                 475                 480 


Pro Gly Leu Ser Asp Glu Lys Leu Asn Leu Thr Ile Gln Asn Asp Ala 
                485                 490                 495     


Tyr Ile Pro Lys Tyr Asp Ser Asn Gly Thr Ser Asp Ile Glu Gln His 
            500                 505                 510         


Asp Val Asn Glu Leu Asn Val Phe Phe Tyr Leu Asp Ala Gln Lys Val 
        515                 520                 525             


Pro Glu Gly Glu Asn Asn Val Asn Leu Thr Ser Ser Ile Asp Thr Ala 
    530                 535                 540                 


Leu Leu Glu Gln Pro Lys Ile Tyr Thr Phe Phe Ser Ser Glu Phe Ile 
545                 550                 555                 560 


Asn Asn Val Asn Lys Pro Val Gln Ala Ala Leu Phe Val Ser Trp Ile 
                565                 570                 575     


Gln Gln Val Leu Val Asp Phe Thr Thr Glu Ala Asn Gln Lys Ser Thr 
            580                 585                 590         


Val Asp Lys Ile Ala Asp Ile Ser Ile Val Val Pro Tyr Ile Gly Leu 
        595                 600                 605             


Ala Leu Asn Ile Gly Asn Glu Ala Gln Lys Gly Asn Phe Lys Asp Ala 
    610                 615                 620                 


Leu Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Glu Pro Glu Leu 
625                 630                 635                 640 


Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe Leu Gly Ser 
                645                 650                 655     


Ser Asp Asn Lys Asn Lys Val Ile Lys Ala Ile Asn Asn Ala Leu Lys 
            660                 665                 670         


Glu Arg Asp Glu Lys Trp Lys Glu Val Tyr Ser Phe Ile Val Ser Asn 
        675                 680                 685             


Trp Met Thr Lys Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu Gln Met 
    690                 695                 700                 


Tyr Gln Ala Leu Gln Asn Gln Val Asn Ala Ile Lys Thr Ile Ile Glu 
705                 710                 715                 720 


Ser Lys Tyr Asn Ser Tyr Thr Leu Glu Glu Lys Asn Glu Leu Thr Asn 
                725                 730                 735     


Lys Tyr Asp Ile Lys Gln Ile Glu Asn Glu Leu Asn Gln Lys Val Ser 
            740                 745                 750         


Ile Ala Met Asn Asn Ile Asp Arg Phe Leu Thr Glu Ser Ser Ile Ser 
        755                 760                 765             


Tyr Leu Met Lys Ile Ile Asn Glu Val Lys Ile Asn Lys Leu Arg Glu 
    770                 775                 780                 


Tyr Asp Glu Asn Val Lys Thr Tyr Leu Leu Asn Tyr Ile Ile Gln His 
785                 790                 795                 800 


Gly Ser Ile Leu Gly Glu Ser Gln Gln Glu Leu Asn Ser Met Val Thr 
                805                 810                 815     


Asp Thr Leu Asn Asn Ser Ile Pro Phe Lys Leu Ser Ser Tyr Thr Asp 
            820                 825                 830         


Asp Lys Ile Leu Ile Ser Tyr Phe Asn Lys Phe Phe Lys Arg Ile Lys 
        835                 840                 845             


Ser Ser Ser Val Leu Asn Met Arg Tyr Lys Asn Asp Lys Tyr Val Asp 
    850                 855                 860                 


Thr Ser Gly Tyr Asp Ser Asn Ile Asn Ile Asn Gly Asp Val Tyr Lys 
865                 870                 875                 880 


Tyr Pro Thr Asn Lys Asn Gln Phe Gly Ile Tyr Asn Asp Lys Leu Ser 
                885                 890                 895     


Glu Val Asn Ile Ser Gln Asn Asp Tyr Ile Ile Tyr Asp Asn Lys Tyr 
            900                 905                 910         


Lys Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Asn Tyr Asp Asn 
        915                 920                 925             


Lys Ile Val Asn Val Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Arg 
    930                 935                 940                 


Asp Asn Asn Ser Gly Trp Lys Val Ser Leu Asn His Asn Glu Ile Ile 
945                 950                 955                 960 


Trp Thr Phe Glu Asp Asn Arg Gly Ile Asn Gln Lys Leu Ala Phe Asn 
                965                 970                 975     


Tyr Gly Asn Ala Asn Gly Ile Ser Asp Tyr Ile Asn Lys Trp Ile Phe 
            980                 985                 990         


Val Thr Ile Thr Asn Asp Arg Leu  Gly Asp Ser Lys Leu  Tyr Ile Asn 
        995                 1000                 1005             


Gly Asn  Leu Ile Asp Gln Lys  Ser Ile Leu Asn Leu  Gly Asn Ile 
    1010                 1015                 1020             


His Val  Ser Asp Asn Ile Leu  Phe Lys Ile Val Asn  Cys Ser Tyr 
    1025                 1030                 1035             


Thr Arg  Tyr Ile Gly Ile Arg  Tyr Phe Asn Ile Phe  Asp Lys Glu 
    1040                 1045                 1050             


Leu Asp  Glu Thr Glu Ile Gln  Thr Leu Tyr Ser Asn  Glu Pro Asn 
    1055                 1060                 1065             


Thr Asn  Ile Leu Lys Asp Phe  Trp Gly Asn Tyr Leu  Leu Tyr Asp 
    1070                 1075                 1080             


Lys Glu  Tyr Tyr Leu Leu Asn  Val Leu Lys Pro Asn  Asn Phe Ile 
    1085                 1090                 1095             


Asp Arg  Arg Lys Asp Ser Thr  Leu Ser Ile Asn Asn  Ile Arg Ser 
    1100                 1105                 1110             


Thr Ile  Leu Leu Ala Asn Arg  Leu Tyr Ser Gly Ile  Lys Val Lys 
    1115                 1120                 1125             


Ile Gln  Arg Val Asn Asn Ser  Ser Thr Asn Asp Asn  Leu Val Arg 
    1130                 1135                 1140             


Lys Asn  Asp Gln Val Tyr Ile  Asn Phe Val Ala Ser  Lys Thr His 
    1145                 1150                 1155             


Leu Phe  Pro Leu Tyr Ala Asp  Thr Ala Thr Thr Asn  Lys Glu Lys 
    1160                 1165                 1170             


Thr Ile  Lys Ile Ser Ser Ser  Gly Asn Arg Phe Asn  Gln Val Val 
    1175                 1180                 1185             


Val Met  Asn Ser Val Gly Asn  Cys Thr Met Asn Phe  Lys Asn Asn 
    1190                 1195                 1200             


Asn Gly  Asn Asn Ile Gly Leu  Leu Gly Phe Lys Ala  Asp Thr Val 
    1205                 1210                 1215             


Val Ala  Ser Thr Trp Tyr Tyr  Thr His Met Arg Asp  His Thr Asn 
    1220                 1225                 1230             


Ser Asn  Gly Cys Phe Trp Asn  Phe Ile Ser Glu Glu  His Gly Trp 
    1235                 1240                 1245             


Gln Glu  Lys 
    1250     


<210>  40
<211>  1278
<212>  PRT
<213>  Clostridium botulinum

<400>  40

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp 
1               5                   10                  15      


Asp Thr Ile Leu Tyr Met Gln Ile Pro Tyr Glu Glu Lys Ser Lys Lys 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Glu Ile Met Arg Asn Val Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Asn Thr Ile Gly Thr Asp Pro Ser Asp Phe Asp Pro Pro Ala Ser 
    50                  55                  60                  


Leu Glu Asn Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr 
65                  70                  75                  80  


Asp Ala Glu Lys Asp Arg Tyr Leu Lys Thr Thr Ile Lys Leu Phe Lys 
                85                  90                  95      


Arg Ile Asn Ser Asn Pro Ala Gly Glu Val Leu Leu Gln Glu Ile Ser 
            100                 105                 110         


Tyr Ala Lys Pro Tyr Leu Gly Asn Glu His Thr Pro Ile Asn Glu Phe 
        115                 120                 125             


His Pro Val Thr Arg Thr Thr Ser Val Asn Ile Lys Ser Ser Thr Asn 
    130                 135                 140                 


Val Lys Ser Ser Ile Ile Leu Asn Leu Leu Val Leu Gly Ala Gly Pro 
145                 150                 155                 160 


Asp Ile Phe Glu Asn Ser Ser Tyr Pro Val Arg Lys Leu Met Asp Ser 
                165                 170                 175     


Gly Gly Val Tyr Asp Pro Ser Asn Asp Gly Phe Gly Ser Ile Asn Ile 
            180                 185                 190         


Val Thr Phe Ser Pro Glu Tyr Glu Tyr Thr Phe Asn Asp Ile Ser Gly 
        195                 200                 205             


Gly Tyr Asn Ser Ser Thr Glu Ser Phe Ile Ala Asp Pro Ala Ile Ser 
    210                 215                 220                 


Leu Ala His Glu Leu Ile His Ala Leu His Gly Leu Tyr Gly Ala Arg 
225                 230                 235                 240 


Gly Val Thr Tyr Lys Glu Thr Ile Lys Val Lys Gln Ala Pro Leu Met 
                245                 250                 255     


Ile Ala Glu Lys Pro Ile Arg Leu Glu Glu Phe Leu Thr Phe Gly Gly 
            260                 265                 270         


Gln Asp Leu Asn Ile Ile Thr Ser Ala Met Lys Glu Lys Ile Tyr Asn 
        275                 280                 285             


Asn Leu Leu Ala Asn Tyr Glu Lys Ile Ala Thr Arg Leu Ser Arg Val 
    290                 295                 300                 


Asn Ser Ala Pro Pro Glu Tyr Asp Ile Asn Glu Tyr Lys Asp Tyr Phe 
305                 310                 315                 320 


Gln Trp Lys Tyr Gly Leu Asp Lys Asn Ala Asp Gly Ser Tyr Thr Val 
                325                 330                 335     


Asn Glu Asn Lys Phe Asn Glu Ile Tyr Lys Lys Leu Tyr Ser Phe Thr 
            340                 345                 350         


Glu Ile Asp Leu Ala Asn Lys Phe Lys Val Lys Cys Arg Asn Thr Tyr 
        355                 360                 365             


Phe Ile Lys Tyr Gly Phe Leu Lys Val Pro Asn Leu Leu Asp Asp Asp 
    370                 375                 380                 


Ile Tyr Thr Val Ser Glu Gly Phe Asn Ile Gly Asn Leu Ala Val Asn 
385                 390                 395                 400 


Asn Arg Gly Gln Asn Ile Lys Leu Asn Pro Lys Ile Ile Asp Ser Ile 
                405                 410                 415     


Pro Asp Lys Gly Leu Val Glu Lys Ile Val Lys Phe Cys Lys Ser Val 
            420                 425                 430         


Ile Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu Cys Ile Arg Val 
        435                 440                 445             


Asn Asn Arg Glu Leu Phe Phe Val Ala Ser Glu Ser Ser Tyr Asn Glu 
    450                 455                 460                 


Asn Asp Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Thr Asn Leu Asn 
465                 470                 475                 480 


Asn Asn Tyr Arg Asn Asn Leu Asp Glu Val Ile Leu Asp Tyr Asn Ser 
                485                 490                 495     


Glu Thr Ile Pro Gln Ile Ser Asn Gln Thr Leu Asn Thr Leu Val Gln 
            500                 505                 510         


Asp Asp Ser Tyr Val Pro Arg Tyr Asp Ser Asn Gly Thr Ser Glu Ile 
        515                 520                 525             


Glu Glu His Asn Val Val Asp Leu Asn Val Phe Phe Tyr Leu His Ala 
    530                 535                 540                 


Gln Lys Val Pro Glu Gly Glu Thr Asn Ile Ser Leu Thr Ser Ser Ile 
545                 550                 555                 560 


Asp Thr Ala Leu Ser Glu Glu Ser Gln Val Tyr Thr Phe Phe Ser Ser 
                565                 570                 575     


Glu Phe Ile Asn Thr Ile Asn Lys Pro Val His Ala Ala Leu Phe Ile 
            580                 585                 590         


Ser Trp Ile Asn Gln Val Ile Arg Asp Phe Thr Thr Glu Ala Thr Gln 
        595                 600                 605             


Lys Ser Thr Phe Asp Lys Ile Ala Asp Ile Ser Leu Val Val Pro Tyr 
    610                 615                 620                 


Val Gly Leu Ala Leu Asn Ile Gly Asn Glu Val Gln Lys Glu Asn Phe 
625                 630                 635                 640 


Lys Glu Ala Phe Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Val 
                645                 650                 655     


Pro Glu Leu Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe 
            660                 665                 670         


Ile Gly Ser Ser Glu Asn Lys Asn Lys Ile Ile Lys Ala Ile Asn Asn 
        675                 680                 685             


Ser Leu Met Glu Arg Glu Thr Lys Trp Lys Glu Ile Tyr Ser Trp Ile 
    690                 695                 700                 


Val Ser Asn Trp Leu Thr Arg Ile Asn Thr Gln Phe Asn Lys Arg Lys 
705                 710                 715                 720 


Glu Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asp Ala Ile Lys Thr 
                725                 730                 735     


Val Ile Glu Tyr Lys Tyr Asn Asn Tyr Thr Ser Asp Glu Arg Asn Arg 
            740                 745                 750         


Leu Glu Ser Glu Tyr Asn Ile Asn Asn Ile Arg Glu Glu Leu Asn Lys 
        755                 760                 765             


Lys Val Ser Leu Ala Met Glu Asn Ile Glu Arg Phe Ile Thr Glu Ser 
    770                 775                 780                 


Ser Ile Phe Tyr Leu Met Lys Leu Ile Asn Glu Ala Lys Val Ser Lys 
785                 790                 795                 800 


Leu Arg Glu Tyr Asp Glu Gly Val Lys Glu Tyr Leu Leu Asp Tyr Ile 
                805                 810                 815     


Ser Glu His Arg Ser Ile Leu Gly Asn Ser Val Gln Glu Leu Asn Asp 
            820                 825                 830         


Leu Val Thr Ser Thr Leu Asn Asn Ser Ile Pro Phe Glu Leu Ser Ser 
        835                 840                 845             


Tyr Thr Asn Asp Lys Ile Leu Ile Leu Tyr Phe Asn Lys Leu Tyr Lys 
    850                 855                 860                 


Lys Ile Lys Asp Asn Ser Ile Leu Asp Met Arg Tyr Glu Asn Asn Lys 
865                 870                 875                 880 


Phe Ile Asp Ile Ser Gly Tyr Gly Ser Asn Ile Ser Ile Asn Gly Asp 
                885                 890                 895     


Val Tyr Ile Tyr Ser Thr Asn Arg Asn Gln Phe Gly Ile Tyr Ser Ser 
            900                 905                 910         


Lys Pro Ser Glu Val Asn Ile Ala Gln Asn Asn Asp Ile Ile Tyr Asn 
        915                 920                 925             


Gly Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Lys 
    930                 935                 940                 


Tyr Phe Asn Lys Val Asn Leu Asn Asn Glu Tyr Thr Ile Ile Asp Cys 
945                 950                 955                 960 


Ile Arg Asn Asn Asn Ser Gly Trp Lys Ile Ser Leu Asn Tyr Asn Lys 
                965                 970                 975     


Ile Ile Trp Thr Leu Gln Asp Thr Ala Gly Asn Asn Gln Lys Leu Val 
            980                 985                 990         


Phe Asn Tyr Thr Gln Met Ile Ser  Ile Ser Asp Tyr Ile  Asn Lys Trp 
        995                 1000                 1005             


Ile Phe  Val Thr Ile Thr Asn  Asn Arg Leu Gly Asn  Ser Arg Ile 
    1010                 1015                 1020             


Tyr Ile  Asn Gly Asn Leu Ile  Asp Glu Lys Ser Ile  Ser Asn Leu 
    1025                 1030                 1035             


Gly Asp  Ile His Val Ser Asp  Asn Ile Leu Phe Lys  Ile Val Gly 
    1040                 1045                 1050             


Cys Asn  Asp Thr Arg Tyr Val  Gly Ile Arg Tyr Phe  Lys Val Phe 
    1055                 1060                 1065             


Asp Thr  Glu Leu Gly Lys Thr  Glu Ile Glu Thr Leu  Tyr Ser Asp 
    1070                 1075                 1080             


Glu Pro  Asp Pro Ser Ile Leu  Lys Asp Phe Trp Gly  Asn Tyr Leu 
    1085                 1090                 1095             


Leu Tyr  Asn Lys Arg Tyr Tyr  Leu Leu Asn Leu Leu  Arg Thr Asp 
    1100                 1105                 1110             


Lys Ser  Ile Thr Gln Asn Ser  Asn Phe Leu Asn Ile  Asn Gln Gln 
    1115                 1120                 1125             


Arg Gly  Val Tyr Gln Lys Pro  Asn Ile Phe Ser Asn  Thr Arg Leu 
    1130                 1135                 1140             


Tyr Thr  Gly Val Glu Val Ile  Ile Arg Lys Asn Gly  Ser Thr Asp 
    1145                 1150                 1155             


Ile Ser  Asn Thr Asp Asn Phe  Val Arg Lys Asn Asp  Leu Ala Tyr 
    1160                 1165                 1170             


Ile Asn  Val Val Asp Arg Asp  Val Glu Tyr Arg Leu  Tyr Ala Asp 
    1175                 1180                 1185             


Ile Ser  Ile Ala Lys Pro Glu  Lys Ile Ile Lys Leu  Ile Arg Thr 
    1190                 1195                 1200             


Ser Asn  Ser Asn Asn Ser Leu  Gly Gln Ile Ile Val  Met Asp Ser 
    1205                 1210                 1215             


Ile Gly  Asn Asn Cys Thr Met  Asn Phe Gln Asn Asn  Asn Gly Gly 
    1220                 1225                 1230             


Asn Ile  Gly Leu Leu Gly Phe  His Ser Asn Asn Leu  Val Ala Ser 
    1235                 1240                 1245             


Ser Trp  Tyr Tyr Asn Asn Ile  Arg Lys Asn Thr Ser  Ser Asn Gly 
    1250                 1255                 1260             


Cys Phe  Trp Ser Phe Ile Ser  Lys Glu His Gly Trp  Gln Glu Asn 
    1265                 1270                 1275             


<210>  41
<211>  1297
<212>  PRT
<213>  Clostridium botulinum


<220>
<221>  SITE
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  41

Met Pro Val Asn Ile Lys Xaa Phe Asn Tyr Asn Asp Pro Ile Asn Asn 
1               5                   10                  15      


Asp Asp Ile Ile Met Met Glu Pro Phe Asn Asp Pro Gly Pro Gly Thr 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Arg Ile Ile Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Phe Thr Tyr Gly Phe Gln Pro Asp Gln Phe Asn Ala Ser Thr Gly 
    50                  55                  60                  


Val Phe Ser Lys Asp Val Tyr Glu Tyr Tyr Asp Pro Thr Tyr Leu Lys 
65                  70                  75                  80  


Thr Asp Ala Glu Lys Asp Lys Phe Leu Lys Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Asn Ser Lys Pro Ser Gly Gln Arg Leu Leu Asp Met Ile 
            100                 105                 110         


Val Asp Ala Ile Pro Tyr Leu Gly Asn Ala Ser Thr Pro Pro Asp Lys 
        115                 120                 125             


Phe Ala Ala Asn Val Ala Asn Val Ser Ile Asn Lys Lys Ile Ile Gln 
    130                 135                 140                 


Pro Gly Ala Glu Asp Gln Ile Lys Gly Leu Met Thr Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Ser Asp Asn Phe Thr Asp Ser Met Ile 
                165                 170                 175     


Met Asn Gly His Ser Pro Ile Ser Glu Gly Phe Gly Ala Arg Met Met 
            180                 185                 190         


Ile Arg Phe Cys Pro Ser Cys Leu Asn Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Asp Thr Ser Ile Phe Ser Arg Arg Ala Tyr Phe Ala Asp Pro 
    210                 215                 220                 


Ala Leu Thr Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Ile Ser Asn Leu Pro Ile Thr Pro Asn Thr Lys Glu Phe 
                245                 250                 255     


Phe Met Gln His Ser Asp Pro Val Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly His Asp Pro Ser Val Ile Ser Pro Ser Thr Asp Met Asn Ile 
        275                 280                 285             


Tyr Asn Lys Ala Leu Gln Asn Phe Gln Asp Ile Ala Asn Arg Leu Asn 
    290                 295                 300                 


Ile Val Ser Ser Ala Gln Gly Ser Gly Ile Asp Ile Ser Leu Tyr Lys 
305                 310                 315                 320 


Gln Ile Tyr Lys Asn Lys Tyr Asp Phe Val Glu Asp Pro Asn Gly Lys 
                325                 330                 335     


Tyr Ser Val Asp Lys Asp Lys Phe Asp Lys Leu Tyr Lys Ala Leu Met 
            340                 345                 350         


Phe Gly Phe Thr Glu Thr Asn Leu Ala Gly Glu Tyr Gly Ile Lys Thr 
        355                 360                 365             


Arg Tyr Ser Tyr Phe Ser Glu Tyr Leu Pro Pro Ile Lys Thr Glu Lys 
    370                 375                 380                 


Leu Leu Asp Asn Thr Ile Tyr Thr Gln Asn Glu Gly Phe Asn Ile Ala 
385                 390                 395                 400 


Ser Lys Asn Leu Lys Thr Glu Phe Asn Gly Gln Asn Lys Ala Val Asn 
                405                 410                 415     


Lys Glu Ala Tyr Glu Glu Ile Ser Leu Glu His Leu Val Ile Tyr Arg 
            420                 425                 430         


Ile Ala Met Cys Lys Pro Val Met Tyr Lys Asn Thr Gly Lys Ser Glu 
        435                 440                 445             


Gln Cys Ile Ile Val Asn Asn Glu Asp Leu Phe Phe Ile Ala Asn Lys 
    450                 455                 460                 


Asp Ser Phe Ser Lys Asp Leu Ala Lys Ala Glu Thr Ile Ala Tyr Asn 
465                 470                 475                 480 


Thr Gln Asn Asn Thr Ile Glu Asn Asn Phe Ser Ile Asp Gln Leu Ile 
                485                 490                 495     


Leu Asp Asn Asp Leu Ser Ser Gly Ile Asp Leu Pro Asn Glu Asn Thr 
            500                 505                 510         


Glu Pro Phe Thr Asn Phe Asp Asp Ile Asp Ile Pro Val Tyr Ile Lys 
        515                 520                 525             


Gln Ser Ala Leu Lys Lys Ile Phe Val Asp Gly Asp Ser Leu Phe Glu 
    530                 535                 540                 


Tyr Leu His Ala Gln Thr Phe Pro Ser Asn Ile Glu Asn Leu Gln Leu 
545                 550                 555                 560 


Thr Asn Ser Leu Asn Asp Ala Leu Arg Asn Asn Asn Lys Val Tyr Thr 
                565                 570                 575     


Phe Phe Ser Thr Asn Leu Val Glu Lys Ala Asn Thr Val Val Gly Ala 
            580                 585                 590         


Ser Leu Phe Val Asn Trp Val Lys Gly Val Ile Asp Asp Phe Thr Ser 
        595                 600                 605             


Glu Ser Thr Gln Lys Ser Thr Ile Asp Lys Val Ser Asp Val Ser Ile 
    610                 615                 620                 


Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Val Gly Asn Glu Thr Ala 
625                 630                 635                 640 


Lys Glu Asn Phe Lys Asn Ala Phe Glu Ile Gly Gly Ala Ala Ile Leu 
                645                 650                 655     


Met Glu Phe Ile Pro Glu Leu Ile Val Pro Ile Val Gly Phe Phe Thr 
            660                 665                 670         


Leu Glu Ser Tyr Val Gly Asn Lys Gly His Ile Ile Met Thr Ile Ser 
        675                 680                 685             


Asn Ala Leu Lys Lys Arg Asp Gln Lys Trp Thr Asp Met Tyr Gly Leu 
    690                 695                 700                 


Ile Val Ser Gln Trp Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile 
705                 710                 715                 720 


Lys Glu Arg Met Tyr Asn Ala Leu Asn Asn Gln Ser Gln Ala Ile Glu 
                725                 730                 735     


Lys Ile Ile Glu Asp Gln Tyr Asn Arg Tyr Ser Glu Glu Asp Lys Met 
            740                 745                 750         


Asn Ile Asn Ile Asp Phe Asn Asp Ile Asp Phe Lys Leu Asn Gln Ser 
        755                 760                 765             


Ile Asn Leu Ala Ile Asn Asn Ile Asp Asp Phe Ile Asn Gln Cys Ser 
    770                 775                 780                 


Ile Ser Tyr Leu Met Asn Arg Met Ile Pro Leu Ala Val Lys Lys Leu 
785                 790                 795                 800 


Lys Asp Phe Asp Asp Asn Leu Lys Arg Asp Leu Leu Glu Tyr Ile Asp 
                805                 810                 815     


Thr Asn Glu Leu Tyr Leu Leu Asp Glu Val Asn Ile Leu Lys Ser Lys 
            820                 825                 830         


Val Asn Arg His Leu Lys Asp Ser Ile Pro Phe Asp Leu Ser Leu Tyr 
        835                 840                 845             


Thr Lys Asp Thr Ile Leu Ile Gln Val Phe Asn Asn Tyr Ile Ser Asn 
    850                 855                 860                 


Ile Ser Ser Asn Ala Ile Leu Ser Leu Ser Tyr Arg Gly Gly Arg Leu 
865                 870                 875                 880 


Ile Asp Ser Ser Gly Tyr Gly Ala Thr Met Asn Val Gly Ser Asp Val 
                885                 890                 895     


Ile Phe Asn Asp Ile Gly Asn Gly Gln Phe Lys Leu Asn Asn Ser Glu 
            900                 905                 910         


Asn Ser Asn Ile Thr Ala His Gln Ser Lys Phe Val Val Tyr Asp Ser 
        915                 920                 925             


Met Phe Asp Asn Phe Ser Ile Asn Phe Trp Val Arg Thr Pro Lys Tyr 
    930                 935                 940                 


Asn Asn Asn Asp Ile Gln Thr Tyr Leu Gln Asn Glu Tyr Thr Ile Ile 
945                 950                 955                 960 


Ser Cys Ile Lys Asn Asp Ser Gly Trp Lys Val Ser Ile Lys Gly Asn 
                965                 970                 975     


Arg Ile Ile Trp Thr Leu Ile Asp Val Asn Ala Lys Ser Lys Ser Ile 
            980                 985                 990         


Phe Phe Glu Tyr Ser Ile Lys Asp  Asn Ile Ser Asp Tyr  Ile Asn Lys 
        995                 1000                 1005             


Trp Phe  Ser Ile Thr Ile Thr  Asn Asp Arg Leu Gly  Asn Ala Asn 
    1010                 1015                 1020             


Ile Tyr  Ile Asn Gly Ser Leu  Lys Lys Ser Glu Lys  Ile Leu Asn 
    1025                 1030                 1035             


Leu Asp  Arg Ile Asn Ser Ser  Asn Asp Ile Asp Phe  Lys Leu Ile 
    1040                 1045                 1050             


Asn Cys  Thr Asp Thr Thr Lys  Phe Val Trp Ile Lys  Asp Phe Asn 
    1055                 1060                 1065             


Ile Phe  Gly Arg Glu Leu Asn  Ala Thr Glu Val Ser  Ser Leu Tyr 
    1070                 1075                 1080             


Trp Ile  Gln Ser Ser Thr Asn  Thr Leu Lys Asp Phe  Trp Gly Asn 
    1085                 1090                 1095             


Pro Leu  Arg Tyr Asp Thr Gln  Tyr Tyr Leu Phe Asn  Gln Gly Met 
    1100                 1105                 1110             


Gln Asn  Ile Tyr Ile Lys Tyr  Phe Ser Lys Ala Ser  Met Gly Glu 
    1115                 1120                 1125             


Thr Ala  Pro Arg Thr Asn Phe  Asn Asn Ala Ala Ile  Asn Tyr Gln 
    1130                 1135                 1140             


Asn Leu  Tyr Leu Gly Leu Arg  Phe Ile Ile Lys Lys  Ala Ser Asn 
    1145                 1150                 1155             


Ser Arg  Asn Ile Asn Asn Asp  Asn Ile Val Arg Glu  Gly Asp Tyr 
    1160                 1165                 1170             


Ile Tyr  Leu Asn Ile Asp Asn  Ile Ser Asp Glu Ser  Tyr Arg Val 
    1175                 1180                 1185             


Tyr Val  Leu Val Asn Ser Lys  Glu Ile Gln Thr Gln  Leu Phe Leu 
    1190                 1195                 1200             


Ala Pro  Ile Asn Asp Asp Pro  Thr Phe Tyr Asp Val  Leu Gln Ile 
    1205                 1210                 1215             


Lys Lys  Tyr Tyr Glu Lys Thr  Thr Tyr Asn Cys Gln  Ile Leu Cys 
    1220                 1225                 1230             


Glu Lys  Asp Thr Lys Thr Phe  Gly Leu Phe Gly Ile  Gly Lys Phe 
    1235                 1240                 1245             


Val Lys  Asp Tyr Gly Tyr Val  Trp Asp Thr Tyr Asp  Asn Tyr Phe 
    1250                 1255                 1260             


Cys Ile  Ser Gln Trp Tyr Leu  Arg Arg Ile Ser Glu  Asn Ile Asn 
    1265                 1270                 1275             


Lys Leu  Arg Leu Gly Cys Asn  Trp Gln Phe Ile Pro  Val Asp Glu 
    1280                 1285                 1290             


Gly Trp  Thr Glu 
    1295         


<210>  42
<211>  1315
<212>  PRT
<213>  Clostridium tetani

<400>  42

Met Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn 
1               5                   10                  15      


Asp Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser 
    50                  55                  60                  


Leu Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr 
65                  70                  75                  80  


Asp Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn 
                85                  90                  95      


Arg Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile 
            100                 105                 110         


Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe 
        115                 120                 125             


Asp Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro 
    130                 135                 140                 


Ser Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe 
145                 150                 155                 160 


Gly Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu 
                165                 170                 175     


Arg Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser 
            180                 185                 190         


Ile Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn 
        195                 200                 205             


Val Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe 
    210                 215                 220                 


Gln Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His 
225                 230                 235                 240 


Gly Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys 
                245                 250                 255     


Gln Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu 
            260                 265                 270         


Phe Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys 
        275                 280                 285             


Asn Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn 
    290                 295                 300                 


Lys Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp 
305                 310                 315                 320 


Ser Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser 
                325                 330                 335     


Asn Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn 
            340                 345                 350         


Ser Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn 
        355                 360                 365             


Ile Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys 
    370                 375                 380                 


Ile Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe 
385                 390                 395                 400 


Asn Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met 
                405                 410                 415     


Arg Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val 
            420                 425                 430         


Ser Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile 
        435                 440                 445             


Arg Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly 
    450                 455                 460                 


Glu Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu 
465                 470                 475                 480 


Lys Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr 
                485                 490                 495     


Asn Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile 
            500                 505                 510         


Ile Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg 
        515                 520                 525             


Thr Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser 
    530                 535                 540                 


Asn Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile 
545                 550                 555                 560 


Tyr Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile 
                565                 570                 575     


Thr Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile 
            580                 585                 590         


Tyr Ser Tyr Phe Pro Ser Val Ile Ser Lys Val Asn Gln Gly Ala Gln 
        595                 600                 605             


Gly Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr 
    610                 615                 620                 


Asn Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser 
625                 630                 635                 640 


Thr Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly 
                645                 650                 655     


Tyr Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu 
            660                 665                 670         


Leu Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu 
        675                 680                 685             


Ser Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile 
    690                 695                 700                 


Asp Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys 
705                 710                 715                 720 


Leu Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys 
                725                 730                 735     


Arg Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile 
            740                 745                 750         


Lys Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys 
        755                 760                 765             


Glu Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu 
    770                 775                 780                 


Lys Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser 
785                 790                 795                 800 


Ser Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln 
                805                 810                 815     


Leu Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile 
            820                 825                 830         


Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu 
        835                 840                 845             


Ser Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser 
    850                 855                 860                 


Lys Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile 
865                 870                 875                 880 


Leu Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile 
                885                 890                 895     


Ser Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala 
            900                 905                 910         


Gln Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn 
        915                 920                 925             


Glu Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn 
    930                 935                 940                 


Asp Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys 
945                 950                 955                 960 


Val Ser Ala Ser His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile 
                965                 970                 975     


Ile Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser 
            980                 985                 990         


Val Ser Leu Lys Gly Asn Asn Leu  Ile Trp Thr Leu Lys  Asp Ser Ala 
        995                 1000                 1005             


Gly Glu  Val Arg Gln Ile Thr  Phe Arg Asp Leu Pro  Asp Lys Phe 
    1010                 1015                 1020             


Asn Ala  Tyr Leu Ala Asn Lys  Trp Val Phe Ile Thr  Ile Thr Asn 
    1025                 1030                 1035             


Asp Arg  Leu Ser Ser Ala Asn  Leu Tyr Ile Asn Gly  Val Leu Met 
    1040                 1045                 1050             


Gly Ser  Ala Glu Ile Thr Gly  Leu Gly Ala Ile Arg  Glu Asp Asn 
    1055                 1060                 1065             


Asn Ile  Thr Leu Lys Leu Asp  Arg Cys Asn Asn Asn  Asn Gln Tyr 
    1070                 1075                 1080             


Val Ser  Ile Asp Lys Phe Arg  Ile Phe Cys Lys Ala  Leu Asn Pro 
    1085                 1090                 1095             


Lys Glu  Ile Glu Lys Leu Tyr  Thr Ser Tyr Leu Ser  Ile Thr Phe 
    1100                 1105                 1110             


Leu Arg  Asp Phe Trp Gly Asn  Pro Leu Arg Tyr Asp  Thr Glu Tyr 
    1115                 1120                 1125             


Tyr Leu  Ile Pro Val Ala Ser  Ser Ser Lys Asp Val  Gln Leu Lys 
    1130                 1135                 1140             


Asn Ile  Thr Asp Tyr Met Tyr  Leu Thr Asn Ala Pro  Ser Tyr Thr 
    1145                 1150                 1155             


Asn Gly  Lys Leu Asn Ile Tyr  Tyr Arg Arg Leu Tyr  Asn Gly Leu 
    1160                 1165                 1170             


Lys Phe  Ile Ile Lys Arg Tyr  Thr Pro Asn Asn Glu  Ile Asp Ser 
    1175                 1180                 1185             


Phe Val  Lys Ser Gly Asp Phe  Ile Lys Leu Tyr Val  Ser Tyr Asn 
    1190                 1195                 1200             


Asn Asn  Glu His Ile Val Gly  Tyr Pro Lys Asp Gly  Asn Ala Phe 
    1205                 1210                 1215             


Asn Asn  Leu Asp Arg Ile Leu  Arg Val Gly Tyr Asn  Ala Pro Gly 
    1220                 1225                 1230             


Ile Pro  Leu Tyr Lys Lys Met  Glu Ala Val Lys Leu  Arg Asp Leu 
    1235                 1240                 1245             


Lys Thr  Tyr Ser Val Gln Leu  Lys Leu Tyr Asp Asp  Lys Asn Ala 
    1250                 1255                 1260             


Ser Leu  Gly Leu Val Gly Thr  His Asn Gly Gln Ile  Gly Asn Asp 
    1265                 1270                 1275             


Pro Asn  Arg Asp Ile Leu Ile  Ala Ser Asn Trp Tyr  Phe Asn His 
    1280                 1285                 1290             


Leu Lys  Asp Lys Ile Leu Gly  Cys Asp Trp Tyr Phe  Val Pro Thr 
    1295                 1300                 1305             


Asp Glu  Gly Trp Thr Asn Asp  
    1310                 1315 


<210>  43
<211>  2634
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LHN/A1 with an EK Cleavage Site

<400>  43
atggagttcg ttaacaaaca gttcaactat aaagacccag ttaacggtgt tgacattgct       60

tacatcaaaa tcccgaacgc tggccagatg cagccggtaa aggcattcaa aatccacaac      120

aaaatctggg ttatcccgga acgtgatacc tttactaacc cggaagaagg tgacctgaac      180

ccgccaccgg aagcgaaaca ggtgccggta tcttactatg actccaccta cctgtctacc      240

gataacgaaa aggacaacta cctgaaaggt gttactaaac tgttcgagcg tatttactcc      300

accgacctgg gccgtatgct gctgactagc atcgttcgcg gtatcccgtt ctggggcggt      360

tctaccatcg ataccgaact gaaagtaatc gacactaact gcatcaacgt tattcagccg      420

gacggttcct atcgttccga agaactgaac ctggtgatca tcggcccgtc tgctgatatc      480

atccagttcg agtgtaagag ctttggtcac gaagttctga acctcacccg taacggctac      540

ggttccactc agtacatccg tttctctccg gacttcacct tcggttttga agaatccctg      600

gaagtagaca cgaacccact gctgggcgct ggtaaattcg caactgatcc tgcggttacc      660

ctggctcacg aactgattca tgcaggccac cgcctgtacg gtatcgccat caatccgaac      720

cgtgtcttca aagttaacac caacgcgtat tacgagatgt ccggtctgga agttagcttc      780

gaagaactgc gtacttttgg cggtcacgac gctaaattca tcgactctct gcaagaaaac      840

gagttccgtc tgtactacta taacaagttc aaagatatcg catccaccct gaacaaagcg      900

aaatccatcg tgggtaccac tgcttctctc cagtacatga agaacgtttt taaagaaaaa      960

tacctgctca gcgaagacac ctccggcaaa ttctctgtag acaagttgaa attcgataaa     1020

ctttacaaaa tgctgactga aatttacacc gaagacaact tcgttaagtt ctttaaagtt     1080

ctgaaccgca aaacctatct gaacttcgac aaggcagtat tcaaaatcaa catcgtgccg     1140

aaagttaact acactatcta cgatggtttc aacctgcgta acaccaacct ggctgctaat     1200

tttaacggcc agaacacgga aatcaacaac atgaacttca caaaactgaa aaacttcact     1260

ggtctgttcg agttttacaa gctgctgtgc gtcgacggca tcattacctc caaaactaaa     1320

tctgacgatg acgataaaaa caaagcgctg aacctgcagt gtatcaaggt taacaactgg     1380

gatttattct tcagcccgag tgaagacaac ttcaccaacg acctgaacaa aggtgaagaa     1440

atcacctcag atactaacat cgaagcagcc gaagaaaaca tctcgctgga cctgatccag     1500

cagtactacc tgacctttaa tttcgacaac gagccggaaa acatttctat cgaaaacctg     1560

agctctgata tcatcggcca gctggaactg atgccgaaca tcgaacgttt cccaaacggt     1620

aaaaagtacg agctggacaa atataccatg ttccactacc tgcgcgcgca ggaatttgaa     1680

cacggcaaat cccgtatcgc actgactaac tccgttaacg aagctctgct caacccgtcc     1740

cgtgtataca ccttcttctc tagcgactac gtgaaaaagg tcaacaaagc gactgaagct     1800

gcaatgttct tgggttgggt tgaacagctt gtttatgatt ttaccgacga gacgtccgaa     1860

gtatctacta ccgacaaaat tgcggatatc actatcatca tcccgtacat cggtccggct     1920

ctgaacattg gcaacatgct gtacaaagac gacttcgttg gcgcactgat cttctccggt     1980

gcggtgatcc tgctggagtt catcccggaa atcgccatcc cggtactggg cacctttgct     2040

ctggtttctt acattgcaaa caaggttctg actgtacaaa ccatcgacaa cgcgctgagc     2100

aaacgtaacg aaaaatggga tgaagtttac aaatatatcg tgaccaactg gctggctaag     2160

gttaatactc agatcgacct catccgcaaa aaaatgaaag aagcactgga aaaccaggcg     2220

gaagctacca aggcaatcat taactaccag tacaaccagt acaccgagga agaaaaaaac     2280

aacatcaact tcaacatcga cgatctgtcc tctaaactga acgaatccat caacaaagct     2340

atgatcaaca tcaacaagtt cctgaaccag tgctctgtaa gctatctgat gaactccatg     2400

atcccgtacg gtgttaaacg tctggaggac ttcgatgcgt ctctgaaaga cgccctgctg     2460

aaatacattt acgacaaccg tggcactctg atcggtcagg ttgatcgtct gaaggacaaa     2520

gtgaacaata ccttatcgac cgacatccct tttcagctca gtaaatatgt cgataaccaa     2580

cgccttttgt ccactctaga agcacaccat catcaccacc atcaccatca ccat           2634


<210>  44
<211>  878
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LHN/A1 with an EK Cleavage Site

<400>  44

Met Glu Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Asp 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Asp Asp Asp Asp Lys Asn Lys 
        435                 440                 445             


Ala Leu Asn Leu Gln Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Leu Glu Ala His His His His His His His His His His 
865                 870                 875             


<210>  45
<211>  3996
<212>  DNA
<213>  Clostridium botulinum

<400>  45
atgaaactgg aaatcaacaa attcaactac aacgatccga tcgatggcat taatgttatt       60

accatgcgtc cgcctcgtca tagcgataaa atcaataaag gtaaaggtcc gttcaaagcc      120

tttcaggtga ttaaaaacat ttggattgtg ccggaacgct acaactttac caataatacc      180

aacgatctga acattccgag cgaaccgatt atggaagcag atgccattta taacccgaac      240

tatctgaata ccccgagcga aaaagatgaa tttctgcagg gtgttatcaa agtgctggaa      300

cgcattaaaa gcaaaccgga aggtgaaaaa ctgctggaac tgattagcag cagcattccg      360

ctgccgctgg ttagcaatgg tgcactgacc ctgagcgata atgaaaccat tgcatatcaa      420

gagaacaaca acattgtgag caatctgcag gcaaacctgg ttatttatgg tccgggtcct      480

gatattgcaa ataatgcaac ctatggtctg tatagcaccc cgattagtaa tggtgaaggt      540

acactgagcg aagttagctt tagcccgttt tatctgaaac cgtttgatga aagctatggc      600

aattatcgta gcctggtgaa tatcgtgaac aaattcgtga aacgtgaatt tgcacctgat      660

ccggcaagca ccctgatgca tgaactggtt catgttaccc ataatctgta tggtattagc      720

aaccgcaact tctactataa ctttgacacc ggcaaaattg aaaccagccg tcagcagaat      780

agcctgattt ttgaagaact gctgaccttt ggtggcattg atagcaaagc aattagcagc      840

ctgatcatca agaaaattat cgaaaccgcc aagaacaact ataccacgct gattagcgaa      900

cgcctgaata ccgttaccgt tgaaaatgat ctgctgaaat atatcaaaaa caaaatcccg      960

gttcagggtc gtctgggtaa ctttaaactg gataccgcag aattcgagaa aaagctgaat     1020

accattctgt ttgtgctgaa cgaaagcaat ctggcacagc gttttagcat tctggttcgt     1080

aaacattacc tgaaagaacg tccgattgat ccgatttatg tgaacattct ggatgacaat     1140

agctacagca ccctggaagg ttttaacatt agcagtcagg gtagcaatga tttccaaggt     1200

cagctgctgg aaagcagcta ttttgaaaaa attgaaagca atgccctgcg tgccttcatt     1260

aaaatctgtc cgcgtaatgg tctgctgtat aatgccattt atcgcaacag caaaaatctg     1320

gaagttctgt ttcagggtcc gcatcatcac caccatcacc atcatcatca cctggaagtg     1380

ttatttcagg gaccgtatct gaataacatt gatctggaag ataaaaagac cacgagcaaa     1440

accaatgtta gctatccgtg tagcctgctg aatggttgta ttgaagttga aaacaaagac     1500

ctgttcctga ttagcaacaa agatagcctg aacgatatta acctgagcga agaaaaaatc     1560

aaaccggaaa ccaccgtgtt cttcaaagat aaactgcctc cgcaggatat tacgctgagc     1620

aattatgatt ttaccgaagc caatagcatt ccgagcatta gccagcagaa cattctggaa     1680

cgtaatgaag aactgtatga accgattcgc aatagcctgt ttgaaatcaa aaccatctat     1740

gtggataagc tgaccacctt tcattttctg gaagcccaga atattgatga gagcattgat     1800

agcagcaaaa ttcgtgttga actgaccgat agcgttgatg aagcactgag caatccgaat     1860

aaagtttata gcccgttcaa gaacatgagc aacaccatta atagcattga aaccggtatt     1920

accagcacct acatctttta tcagtggctg cgtagcatcg tgaaagattt tagtgatgaa     1980

accggcaaaa tcgacgtgat tgataaaagc agcgataccc tggcaattgt tccgtatatt     2040

ggtccgctgc tgaatattgg taatgatatt cgtcatggcg attttgtggg tgcaattgaa     2100

ctggcaggca ttaccgcact gctggaatat gttccggaat ttaccattcc gattctggtt     2160

ggtctggaag tgattggtgg cgaactggca cgtgaacagg ttgaagcaat tgttaataat     2220

gccctggata aacgcgatca gaaatgggca gaagtttaca atattaccaa agcacagtgg     2280

tggggcacca ttcatttaca gattaatacc cgtctggccc atacctataa agccctgagc     2340

cgtcaggcaa atgccattaa aatgaatatg gaatttcagc tggccaacta caaaggcaac     2400

attgatgata aagccaagat caaaaacgcc atcagcgaaa ccgaaattct gctgaacaaa     2460

agcgttgaac aggccatgaa aaacaccgag aaattcatga ttaaactgag caacagctac     2520

ctgaccaaag aaatgattcc gaaagttcag gacaacctga aaaactttga cctggaaacc     2580

aaaaaaaccc tggacaagtt catcaaagag aaagaagata tcctgggcac caatctgagc     2640

agcagcctgc gtcgtaaagt tagcattcgt ctgaataaaa acattgcctt cgacatcaac     2700

gatatcccgt ttagcgaatt tgatgatctg atcaaccagt acaaaaacga gatcgaagat     2760

tatgaagtgc tgaatctggg tgcagaagat ggcaaaatca aagatctgag cggtacaacc     2820

agcgatatca atattggttc agatatcgaa ctggccgatg gtcgtgaaaa taaagccatt     2880

aagattaaag gcagcgagaa cagcaccatc aaaattgcaa tgaacaaata tctgcgtttt     2940

agcgccaccg ataactttag cattagcttt tggatcaaac atccgaaacc gaccaatctg     3000

cttaataacg gtattgaata taccctggtc gagaacttta atcagcgtgg ttggaaaatt     3060

agcatccagg atagcaaact gatttggtat ctgcgcgatc acaataacag catcaaaatc     3120

gttacaccgg attatattgc gtttaatggc tggaacctga tcaccattac gaataatcgt     3180

agcaaaggca gcatcgtgta tgtgaatggt agcaaaattg aagagaagga cattagcagc     3240

atttggaata ccgaagtgga tgatccgatt atcttccgcc tgaaaaataa ccgtgatacc     3300

caggcattta ccctgctgga tcagtttagc atttatcgga aagaactgaa ccagaacgaa     3360

gtggtgaaac tgtataacta ctacttcaac agcaactaca ttcgcgatat ttggggtaat     3420

ccgctgcagt acaacaaaaa atactatctg cagacccagg acaaacctgg taaaggtctg     3480

atccgcgaat attggagcag ctttggttat gattatgtga ttctgagcga tagcaagacg     3540

attacctttc cgaacaatat ccgttatggt gccctgtata acggtagcaa agttctgatc     3600

aagaacagca agaaattaga tggtctggtg cgcaataaag atttcattca gctggaaatc     3660

gatggctata atatgggtat tagcgcagat cgctttaacg aggataccaa ctatattggc     3720

accacctatg gtacaaccca tgatctgacc accgattttg aaattattca gcgccaagag     3780

aaataccgca attattgtca gctgaaaacc ccgtataaca tctttcataa aagcggtctg     3840

atgagcaccg aaaccagcaa accgaccttc catgattatc gcgattgggt ttatagcagc     3900

gcatggtatt ttcagaacta tgaaaatctg aacctgcgca aacataccaa aaccaactgg     3960

tattttatcc cgaaagatga aggttgggac gaagat                               3996


<210>  46
<211>  1332
<212>  PRT
<213>  Clostridium botulinum

<400>  46

Met Lys Leu Glu Ile Asn Lys Phe Asn Tyr Asn Asp Pro Ile Asp Gly 
1               5                   10                  15      


Ile Asn Val Ile Thr Met Arg Pro Pro Arg His Ser Asp Lys Ile Asn 
            20                  25                  30          


Lys Gly Lys Gly Pro Phe Lys Ala Phe Gln Val Ile Lys Asn Ile Trp 
        35                  40                  45              


Ile Val Pro Glu Arg Tyr Asn Phe Thr Asn Asn Thr Asn Asp Leu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Pro Ile Met Glu Ala Asp Ala Ile Tyr Asn Pro Asn 
65                  70                  75                  80  


Tyr Leu Asn Thr Pro Ser Glu Lys Asp Glu Phe Leu Gln Gly Val Ile 
                85                  90                  95      


Lys Val Leu Glu Arg Ile Lys Ser Lys Pro Glu Gly Glu Lys Leu Leu 
            100                 105                 110         


Glu Leu Ile Ser Ser Ser Ile Pro Leu Pro Leu Val Ser Asn Gly Ala 
        115                 120                 125             


Leu Thr Leu Ser Asp Asn Glu Thr Ile Ala Tyr Gln Glu Asn Asn Asn 
    130                 135                 140                 


Ile Val Ser Asn Leu Gln Ala Asn Leu Val Ile Tyr Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Ile Ala Asn Asn Ala Thr Tyr Gly Leu Tyr Ser Thr Pro Ile Ser 
                165                 170                 175     


Asn Gly Glu Gly Thr Leu Ser Glu Val Ser Phe Ser Pro Phe Tyr Leu 
            180                 185                 190         


Lys Pro Phe Asp Glu Ser Tyr Gly Asn Tyr Arg Ser Leu Val Asn Ile 
        195                 200                 205             


Val Asn Lys Phe Val Lys Arg Glu Phe Ala Pro Asp Pro Ala Ser Thr 
    210                 215                 220                 


Leu Met His Glu Leu Val His Val Thr His Asn Leu Tyr Gly Ile Ser 
225                 230                 235                 240 


Asn Arg Asn Phe Tyr Tyr Asn Phe Asp Thr Gly Lys Ile Glu Thr Ser 
                245                 250                 255     


Arg Gln Gln Asn Ser Leu Ile Phe Glu Glu Leu Leu Thr Phe Gly Gly 
            260                 265                 270         


Ile Asp Ser Lys Ala Ile Ser Ser Leu Ile Ile Lys Lys Ile Ile Glu 
        275                 280                 285             


Thr Ala Lys Asn Asn Tyr Thr Thr Leu Ile Ser Glu Arg Leu Asn Thr 
    290                 295                 300                 


Val Thr Val Glu Asn Asp Leu Leu Lys Tyr Ile Lys Asn Lys Ile Pro 
305                 310                 315                 320 


Val Gln Gly Arg Leu Gly Asn Phe Lys Leu Asp Thr Ala Glu Phe Glu 
                325                 330                 335     


Lys Lys Leu Asn Thr Ile Leu Phe Val Leu Asn Glu Ser Asn Leu Ala 
            340                 345                 350         


Gln Arg Phe Ser Ile Leu Val Arg Lys His Tyr Leu Lys Glu Arg Pro 
        355                 360                 365             


Ile Asp Pro Ile Tyr Val Asn Ile Leu Asp Asp Asn Ser Tyr Ser Thr 
    370                 375                 380                 


Leu Glu Gly Phe Asn Ile Ser Ser Gln Gly Ser Asn Asp Phe Gln Gly 
385                 390                 395                 400 


Gln Leu Leu Glu Ser Ser Tyr Phe Glu Lys Ile Glu Ser Asn Ala Leu 
                405                 410                 415     


Arg Ala Phe Ile Lys Ile Cys Pro Arg Asn Gly Leu Leu Tyr Asn Ala 
            420                 425                 430         


Ile Tyr Arg Asn Ser Lys Asn Leu Glu Val Leu Phe Gln Gly Pro His 
        435                 440                 445             


His His His His His His His His His Leu Glu Val Leu Phe Gln Gly 
    450                 455                 460                 


Pro Tyr Leu Asn Asn Ile Asp Leu Glu Asp Lys Lys Thr Thr Ser Lys 
465                 470                 475                 480 


Thr Asn Val Ser Tyr Pro Cys Ser Leu Leu Asn Gly Cys Ile Glu Val 
                485                 490                 495     


Glu Asn Lys Asp Leu Phe Leu Ile Ser Asn Lys Asp Ser Leu Asn Asp 
            500                 505                 510         


Ile Asn Leu Ser Glu Glu Lys Ile Lys Pro Glu Thr Thr Val Phe Phe 
        515                 520                 525             


Lys Asp Lys Leu Pro Pro Gln Asp Ile Thr Leu Ser Asn Tyr Asp Phe 
    530                 535                 540                 


Thr Glu Ala Asn Ser Ile Pro Ser Ile Ser Gln Gln Asn Ile Leu Glu 
545                 550                 555                 560 


Arg Asn Glu Glu Leu Tyr Glu Pro Ile Arg Asn Ser Leu Phe Glu Ile 
                565                 570                 575     


Lys Thr Ile Tyr Val Asp Lys Leu Thr Thr Phe His Phe Leu Glu Ala 
            580                 585                 590         


Gln Asn Ile Asp Glu Ser Ile Asp Ser Ser Lys Ile Arg Val Glu Leu 
        595                 600                 605             


Thr Asp Ser Val Asp Glu Ala Leu Ser Asn Pro Asn Lys Val Tyr Ser 
    610                 615                 620                 


Pro Phe Lys Asn Met Ser Asn Thr Ile Asn Ser Ile Glu Thr Gly Ile 
625                 630                 635                 640 


Thr Ser Thr Tyr Ile Phe Tyr Gln Trp Leu Arg Ser Ile Val Lys Asp 
                645                 650                 655     


Phe Ser Asp Glu Thr Gly Lys Ile Asp Val Ile Asp Lys Ser Ser Asp 
            660                 665                 670         


Thr Leu Ala Ile Val Pro Tyr Ile Gly Pro Leu Leu Asn Ile Gly Asn 
        675                 680                 685             


Asp Ile Arg His Gly Asp Phe Val Gly Ala Ile Glu Leu Ala Gly Ile 
    690                 695                 700                 


Thr Ala Leu Leu Glu Tyr Val Pro Glu Phe Thr Ile Pro Ile Leu Val 
705                 710                 715                 720 


Gly Leu Glu Val Ile Gly Gly Glu Leu Ala Arg Glu Gln Val Glu Ala 
                725                 730                 735     


Ile Val Asn Asn Ala Leu Asp Lys Arg Asp Gln Lys Trp Ala Glu Val 
            740                 745                 750         


Tyr Asn Ile Thr Lys Ala Gln Trp Trp Gly Thr Ile His Leu Gln Ile 
        755                 760                 765             


Asn Thr Arg Leu Ala His Thr Tyr Lys Ala Leu Ser Arg Gln Ala Asn 
    770                 775                 780                 


Ala Ile Lys Met Asn Met Glu Phe Gln Leu Ala Asn Tyr Lys Gly Asn 
785                 790                 795                 800 


Ile Asp Asp Lys Ala Lys Ile Lys Asn Ala Ile Ser Glu Thr Glu Ile 
                805                 810                 815     


Leu Leu Asn Lys Ser Val Glu Gln Ala Met Lys Asn Thr Glu Lys Phe 
            820                 825                 830         


Met Ile Lys Leu Ser Asn Ser Tyr Leu Thr Lys Glu Met Ile Pro Lys 
        835                 840                 845             


Val Gln Asp Asn Leu Lys Asn Phe Asp Leu Glu Thr Lys Lys Thr Leu 
    850                 855                 860                 


Asp Lys Phe Ile Lys Glu Lys Glu Asp Ile Leu Gly Thr Asn Leu Ser 
865                 870                 875                 880 


Ser Ser Leu Arg Arg Lys Val Ser Ile Arg Leu Asn Lys Asn Ile Ala 
                885                 890                 895     


Phe Asp Ile Asn Asp Ile Pro Phe Ser Glu Phe Asp Asp Leu Ile Asn 
            900                 905                 910         


Gln Tyr Lys Asn Glu Ile Glu Asp Tyr Glu Val Leu Asn Leu Gly Ala 
        915                 920                 925             


Glu Asp Gly Lys Ile Lys Asp Leu Ser Gly Thr Thr Ser Asp Ile Asn 
    930                 935                 940                 


Ile Gly Ser Asp Ile Glu Leu Ala Asp Gly Arg Glu Asn Lys Ala Ile 
945                 950                 955                 960 


Lys Ile Lys Gly Ser Glu Asn Ser Thr Ile Lys Ile Ala Met Asn Lys 
                965                 970                 975     


Tyr Leu Arg Phe Ser Ala Thr Asp Asn Phe Ser Ile Ser Phe Trp Ile 
            980                 985                 990         


Lys His Pro Lys Pro Thr Asn Leu  Leu Asn Asn Gly Ile  Glu Tyr Thr 
        995                 1000                 1005             


Leu Val  Glu Asn Phe Asn Gln  Arg Gly Trp Lys Ile  Ser Ile Gln 
    1010                 1015                 1020             


Asp Ser  Lys Leu Ile Trp Tyr  Leu Arg Asp His Asn  Asn Ser Ile 
    1025                 1030                 1035             


Lys Ile  Val Thr Pro Asp Tyr  Ile Ala Phe Asn Gly  Trp Asn Leu 
    1040                 1045                 1050             


Ile Thr  Ile Thr Asn Asn Arg  Ser Lys Gly Ser Ile  Val Tyr Val 
    1055                 1060                 1065             


Asn Gly  Ser Lys Ile Glu Glu  Lys Asp Ile Ser Ser  Ile Trp Asn 
    1070                 1075                 1080             


Thr Glu  Val Asp Asp Pro Ile  Ile Phe Arg Leu Lys  Asn Asn Arg 
    1085                 1090                 1095             


Asp Thr  Gln Ala Phe Thr Leu  Leu Asp Gln Phe Ser  Ile Tyr Arg 
    1100                 1105                 1110             


Lys Glu  Leu Asn Gln Asn Glu  Val Val Lys Leu Tyr  Asn Tyr Tyr 
    1115                 1120                 1125             


Phe Asn  Ser Asn Tyr Ile Arg  Asp Ile Trp Gly Asn  Pro Leu Gln 
    1130                 1135                 1140             


Tyr Asn  Lys Lys Tyr Tyr Leu  Gln Thr Gln Asp Lys  Pro Gly Lys 
    1145                 1150                 1155             


Gly Leu  Ile Arg Glu Tyr Trp  Ser Ser Phe Gly Tyr  Asp Tyr Val 
    1160                 1165                 1170             


Ile Leu  Ser Asp Ser Lys Thr  Ile Thr Phe Pro Asn  Asn Ile Arg 
    1175                 1180                 1185             


Tyr Gly  Ala Leu Tyr Asn Gly  Ser Lys Val Leu Ile  Lys Asn Ser 
    1190                 1195                 1200             


Lys Lys  Leu Asp Gly Leu Val  Arg Asn Lys Asp Phe  Ile Gln Leu 
    1205                 1210                 1215             


Glu Ile  Asp Gly Tyr Asn Met  Gly Ile Ser Ala Asp  Arg Phe Asn 
    1220                 1225                 1230             


Glu Asp  Thr Asn Tyr Ile Gly  Thr Thr Tyr Gly Thr  Thr His Asp 
    1235                 1240                 1245             


Leu Thr  Thr Asp Phe Glu Ile  Ile Gln Arg Gln Glu  Lys Tyr Arg 
    1250                 1255                 1260             


Asn Tyr  Cys Gln Leu Lys Thr  Pro Tyr Asn Ile Phe  His Lys Ser 
    1265                 1270                 1275             


Gly Leu  Met Ser Thr Glu Thr  Ser Lys Pro Thr Phe  His Asp Tyr 
    1280                 1285                 1290             


Arg Asp  Trp Val Tyr Ser Ser  Ala Trp Tyr Phe Gln  Asn Tyr Glu 
    1295                 1300                 1305             


Asn Leu  Asn Leu Arg Lys His  Thr Lys Thr Asn Trp  Tyr Phe Ile 
    1310                 1315                 1320             


Pro Lys  Asp Glu Gly Trp Asp  Glu Asp 
    1325                 1330         


<210>  47
<211>  247
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Trypsin

<400>  47

Met His Pro Leu Leu Ile Leu Ala Phe Val Gly Ala Ala Val Ala Phe 
1               5                   10                  15      


Pro Ser Asp Asp Asp Asp Lys Ile Val Gly Gly Tyr Thr Cys Ala Glu 
            20                  25                  30          


Asn Ser Val Pro Tyr Gln Val Ser Leu Asn Ala Gly Tyr His Phe Cys 
        35                  40                  45              


Gly Gly Ser Leu Ile Asn Asp Gln Trp Val Val Ser Ala Ala His Cys 
    50                  55                  60                  


Tyr Gln Tyr His Ile Gln Val Arg Leu Gly Glu Tyr Asn Ile Asp Val 
65                  70                  75                  80  


Leu Glu Gly Gly Glu Gln Phe Ile Asp Ala Ser Lys Ile Ile Arg His 
                85                  90                  95      


Pro Lys Tyr Ser Ser Trp Thr Leu Asp Asn Asp Ile Leu Leu Ile Lys 
            100                 105                 110         


Leu Ser Thr Pro Ala Val Ile Asn Ala Arg Val Ser Thr Leu Ala Leu 
        115                 120                 125             


Pro Ser Ala Cys Ala Ser Gly Ser Thr Glu Cys Leu Ile Ser Gly Trp 
    130                 135                 140                 


Gly Asn Thr Leu Ser Ser Gly Val Asn Tyr Pro Asp Leu Leu Gln Cys 
145                 150                 155                 160 


Leu Glu Ala Pro Leu Leu Ser His Ala Asp Cys Glu Ala Ser Tyr Pro 
                165                 170                 175     


Gly Glu Ile Thr Asn Asn Met Ile Cys Ala Gly Phe Leu Glu Gly Gly 
            180                 185                 190         


Lys Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro Val Ala Cys Asn Gly 
        195                 200                 205             


Gln Leu Gln Gly Ile Val Ser Trp Gly Tyr Gly Cys Ala Gln Lys Gly 
    210                 215                 220                 


Lys Pro Gly Val Tyr Thr Lys Val Cys Asn Tyr Val Asp Trp Ile Gln 
225                 230                 235                 240 


Glu Thr Ile Ala Ala Asn Ser 
                245         


<210>  48
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lys-C

<400>  48

Gly Val Ser Gly Ser Cys Asn Ile Asp Val Val Cys Pro Glu Gly Asn 
1               5                   10                  15      


Gly His Arg Asp Val Ile Arg Ser Val Ala Ala Tyr Ser Lys Gln Gly 
            20                  25                  30          


Thr Met Trp Cys Thr Gly Ser Leu Val Asn Asn Ser Ala Asn Asp Lys 
        35                  40                  45              


Lys Met Tyr Phe Leu Thr Ala Asn His Cys Gly Met Thr Thr Ala Ala 
    50                  55                  60                  


Ile Ala Ser Ser Met Val Val Tyr Trp Asn Tyr Gln Asn Ser Thr Cys 
65                  70                  75                  80  


Arg Ala Pro Gly Ser Ser Ser Ser Gly Ala Asn Gly Asp Gly Ser Leu 
                85                  90                  95      


Ala Gln Ser Gln Thr Gly Ala Val Val Arg Ala Thr Asn Ala Ala Ser 
            100                 105                 110         


Asp Phe Thr Leu Leu Glu Leu Asn Thr Ala Ala Asn Pro Ala Tyr Asn 
        115                 120                 125             


Leu Phe Trp Ala Gly Trp Asp Arg Arg Asp Gln Asn Phe Ala Gly Ala 
    130                 135                 140                 


Thr Ala Ile His His Pro Asn Val Ala Glu Lys Arg Ile Ser His Ser 
145                 150                 155                 160 


Thr Val Ala Thr Glu Ile Ser Gly Tyr Asn Gly Ala Thr Gly Thr Ser 
                165                 170                 175     


His Leu His Val Phe Trp Gln Ala Ser Gly Gly Val Thr Glu Pro Gly 
            180                 185                 190         


Ser Ser Gly Ser Pro Ile Tyr Ser Pro Glu Lys Arg Val Leu Gly Gln 
        195                 200                 205             


Leu His Gly Gly Pro Ser Ser Cys Ser Ala Thr Gly Ala Asp Arg Ser 
    210                 215                 220                 


Asp Tyr Tyr Gly Arg Val Phe Thr Ser Trp Thr Gly Gly Gly Thr Ser 
225                 230                 235                 240 


Ala Thr Arg Leu Ser Asp Trp Leu Asp Ala Ala Gly Thr Gly Ala Gln 
                245                 250                 255     


Phe Ile Asp Gly Leu Asp Ser Thr Gly Thr Pro Pro Val 
            260                 265                 


<210>  49
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Enterokinase Light Chain

<400>  49

Ile Val Gly Gly Ser Asp Ser Arg Glu Gly Ala Trp Pro Trp Val Val 
1               5                   10                  15      


Ala Leu Tyr Phe Asp Asp Gln Gln Val Cys Gly Ala Ser Leu Val Ser 
            20                  25                  30          


Arg Asp Trp Leu Val Ser Ala Ala His Cys Val Tyr Gly Arg Asn Met 
        35                  40                  45              


Glu Pro Ser Lys Trp Lys Ala Val Leu Gly Leu His Met Ala Ser Asn 
    50                  55                  60                  


Leu Thr Ser Pro Gln Ile Glu Thr Arg Leu Ile Asp Gln Ile Val Ile 
65                  70                  75                  80  


Asn Arg His Tyr Asn Lys Arg Arg Lys Asn Asn Asp Ile Ala Met Met 
                85                  90                  95      


His Leu Glu Met Lys Val Asn Tyr Thr Asp Tyr Ile Gln Pro Ile Cys 
            100                 105                 110         


Leu Pro Glu Glu Asn Gln Val Phe Pro Pro Gly Arg Ile Cys Ser Ile 
        115                 120                 125             


Ala Gly Trp Gly Ala Leu Ile Tyr Gln Gly Ser Thr Ala Asp Val Leu 
    130                 135                 140                 


Gln Glu Ala Asp Val Pro Leu Leu Ser Asn Glu Lys Cys Gln Gln Gln 
145                 150                 155                 160 


Met Pro Glu Tyr Asn Ile Thr Glu Asn Met Val Cys Ala Gly Tyr Asp 
                165                 170                 175     


Ala Gly Gly Val Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro Leu Met 
            180                 185                 190         


Cys Gln Glu Asn Asn Arg Trp Leu Leu Ala Gly Val Thr Ser Phe Gly 
        195                 200                 205             


Tyr Gln Cys Ala Leu Pro Asn Arg Pro Gly Val Tyr Ala Arg Val Pro 
    210                 215                 220                 


Arg Phe Thr Glu Trp Ile Gln Ser Phe Leu His 
225                 230                 235 


<210>  50
<211>  259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Factor Xa Heavy Chain

<400>  50

Ile Val Gly Gly Arg Asp Cys Ala Glu Gly Glu Cys Pro Trp Gln Ala 
1               5                   10                  15      


Leu Leu Val Asn Glu Glu Asn Glu Gly Phe Cys Gly Gly Thr Ile Leu 
            20                  25                  30          


Asn Glu Phe Tyr Val Leu Thr Ala Ala His Cys Leu His Gln Ala Lys 
        35                  40                  45              


Arg Phe Thr Val Arg Val Gly Asp Arg Asn Thr Glu Gln Glu Glu Gly 
    50                  55                  60                  


Asn Glu Met Ala His Glu Val Glu Met Thr Val Lys His Ser Arg Phe 
65                  70                  75                  80  


Val Lys Glu Thr Tyr Asp Phe Asp Ile Ala Val Leu Arg Leu Lys Thr 
                85                  90                  95      


Pro Ile Arg Phe Arg Arg Asn Val Ala Pro Ala Cys Leu Pro Glu Lys 
            100                 105                 110         


Asp Trp Ala Glu Ala Thr Leu Met Thr Gln Lys Thr Gly Ile Val Ser 
        115                 120                 125             


Gly Phe Gly Arg Thr His Glu Lys Gly Arg Leu Ser Ser Thr Leu Lys 
    130                 135                 140                 


Met Leu Glu Val Pro Tyr Val Asp Arg Ser Thr Cys Lys Leu Ser Ser 
145                 150                 155                 160 


Ser Phe Thr Ile Thr Pro Asn Met Phe Cys Ala Gly Tyr Asp Thr Gln 
                165                 170                 175     


Pro Glu Asp Ala Cys Gln Gly Asp Ser Gly Gly Pro His Val Thr Arg 
            180                 185                 190         


Phe Lys Asp Thr Tyr Phe Val Thr Gly Ile Val Ser Trp Gly Glu Gly 
        195                 200                 205             


Cys Ala Arg Lys Gly Lys Phe Gly Val Tyr Thr Lys Val Ser Asn Phe 
    210                 215                 220                 


Leu Lys Trp Ile Asp Lys Ile Met Lys Ala Arg Ala Gly Ala Ala Gly 
225                 230                 235                 240 


Ser Arg Gly His Ser Glu Ala Pro Ala Thr Trp Thr Val Pro Pro Pro 
                245                 250                 255     


Leu Pro Leu 
            


<210>  51
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Factor Xa Light Chain

<400>  51

Ala Asn Ser Phe Leu Glu Glu Val Lys Gln Gly Asn Leu Glu Arg Glu 
1               5                   10                  15      


Cys Leu Glu Glu Ala Cys Ser Leu Glu Glu Ala Arg Glu Val Phe Glu 
            20                  25                  30          


Asp Ala Glu Gln Thr Asp Glu Phe Trp Ser Lys Tyr Lys Asp Gly Asp 
        35                  40                  45              


Gln Cys Glu Gly His Pro Cys Leu Asn Gln Gly His Cys Lys Asp Gly 
    50                  55                  60                  


Ile Gly Asp Tyr Thr Cys Thr Cys Ala Glu Gly Phe Glu Gly Lys Asn 
65                  70                  75                  80  


Cys Glu Phe Ser Thr Arg Glu Ile Cys Ser Leu Asp Asn Gly Gly Cys 
                85                  90                  95      


Asp Gln Phe Cys Arg Glu Glu Arg Ser Glu Val Arg Cys Ser Cys Ala 
            100                 105                 110         


His Gly Tyr Val Leu Gly Asp Asp Ser Lys Ser Cys Val Ser Thr Glu 
        115                 120                 125             


Arg Phe Pro Cys Gly Lys Phe Thr Gln Gly Arg Ser 
    130                 135                 140 


<210>  52
<211>  764
<212>  PRT
<213>  Bacillus anthracis

<400>  52

Met Lys Lys Arg Lys Val Leu Ile Pro Leu Met Ala Leu Ser Thr Ile 
1               5                   10                  15      


Leu Val Ser Ser Thr Gly Asn Leu Glu Val Ile Gln Ala Glu Val Lys 
            20                  25                  30          


Gln Glu Asn Arg Leu Leu Asn Glu Ser Glu Ser Ser Ser Gln Gly Leu 
        35                  40                  45              


Leu Gly Tyr Tyr Phe Ser Asp Leu Asn Phe Gln Ala Pro Met Val Val 
    50                  55                  60                  


Thr Ser Ser Thr Thr Gly Asp Leu Ser Ile Pro Ser Ser Glu Leu Glu 
65                  70                  75                  80  


Asn Ile Pro Ser Glu Asn Gln Tyr Phe Gln Ser Ala Ile Trp Ser Gly 
                85                  90                  95      


Phe Ile Lys Val Lys Lys Ser Asp Glu Tyr Thr Phe Ala Thr Ser Ala 
            100                 105                 110         


Asp Asn His Val Thr Met Trp Val Asp Asp Gln Glu Val Ile Asn Lys 
        115                 120                 125             


Ala Ser Asn Ser Asn Lys Ile Arg Leu Glu Lys Gly Arg Leu Tyr Gln 
    130                 135                 140                 


Ile Lys Ile Gln Tyr Gln Arg Glu Asn Pro Thr Glu Lys Gly Leu Asp 
145                 150                 155                 160 


Phe Lys Leu Tyr Trp Thr Asp Ser Gln Asn Lys Lys Glu Val Ile Ser 
                165                 170                 175     


Ser Asp Asn Leu Gln Leu Pro Glu Leu Lys Gln Lys Ser Ser Asn Ser 
            180                 185                 190         


Arg Lys Lys Arg Ser Thr Ser Ala Gly Pro Thr Val Pro Asp Arg Asp 
        195                 200                 205             


Asn Asp Gly Ile Pro Asp Ser Leu Glu Val Glu Gly Tyr Thr Val Asp 
    210                 215                 220                 


Val Lys Asn Lys Arg Thr Phe Leu Ser Pro Trp Ile Ser Asn Ile His 
225                 230                 235                 240 


Glu Lys Lys Gly Leu Thr Lys Tyr Lys Ser Ser Pro Glu Lys Trp Ser 
                245                 250                 255     


Thr Ala Ser Asp Pro Tyr Ser Asp Phe Glu Lys Val Thr Gly Arg Ile 
            260                 265                 270         


Asp Lys Asn Val Ser Pro Glu Ala Arg His Pro Leu Val Ala Ala Tyr 
        275                 280                 285             


Pro Ile Val His Val Asp Met Glu Asn Ile Ile Leu Ser Lys Asn Glu 
    290                 295                 300                 


Asp Gln Ser Thr Gln Asn Thr Asp Ser Gln Thr Arg Thr Ile Ser Lys 
305                 310                 315                 320 


Asn Thr Ser Thr Ser Arg Thr His Thr Ser Glu Val His Gly Asn Ala 
                325                 330                 335     


Glu Val His Ala Ser Phe Phe Asp Ile Gly Gly Ser Val Ser Ala Gly 
            340                 345                 350         


Phe Ser Asn Ser Asn Ser Ser Thr Val Ala Ile Asp His Ser Leu Ser 
        355                 360                 365             


Leu Ala Gly Glu Arg Thr Trp Ala Glu Thr Met Gly Leu Asn Thr Ala 
    370                 375                 380                 


Asp Thr Ala Arg Leu Asn Ala Asn Ile Arg Tyr Val Asn Thr Gly Thr 
385                 390                 395                 400 


Ala Pro Ile Tyr Asn Val Leu Pro Thr Thr Ser Leu Val Leu Gly Lys 
                405                 410                 415     


Asn Gln Thr Leu Ala Thr Ile Lys Ala Lys Glu Asn Gln Leu Ser Gln 
            420                 425                 430         


Ile Leu Ala Pro Asn Asn Tyr Tyr Pro Ser Lys Asn Leu Ala Pro Ile 
        435                 440                 445             


Ala Leu Asn Ala Gln Asp Asp Phe Ser Ser Thr Pro Ile Thr Met Asn 
    450                 455                 460                 


Tyr Asn Gln Phe Leu Glu Leu Glu Lys Thr Lys Gln Leu Arg Leu Asp 
465                 470                 475                 480 


Thr Asp Gln Val Tyr Gly Asn Ile Ala Thr Tyr Asn Phe Glu Asn Gly 
                485                 490                 495     


Arg Val Arg Val Asp Thr Gly Ser Asn Trp Ser Glu Val Leu Pro Gln 
            500                 505                 510         


Ile Gln Glu Thr Thr Ala Arg Ile Ile Phe Asn Gly Lys Asp Leu Asn 
        515                 520                 525             


Leu Val Glu Arg Arg Ile Ala Ala Val Asn Pro Ser Asp Pro Leu Glu 
    530                 535                 540                 


Thr Thr Lys Pro Asp Met Thr Leu Lys Glu Ala Leu Lys Ile Ala Phe 
545                 550                 555                 560 


Gly Phe Asn Glu Pro Asn Gly Asn Leu Gln Tyr Gln Gly Lys Asp Ile 
                565                 570                 575     


Thr Glu Phe Asp Phe Asn Phe Asp Gln Gln Thr Ser Gln Asn Ile Lys 
            580                 585                 590         


Asn Gln Leu Ala Glu Leu Asn Ala Thr Asn Ile Tyr Thr Val Leu Asp 
        595                 600                 605             


Lys Ile Lys Leu Asn Ala Lys Met Asn Ile Leu Ile Arg Asp Lys Arg 
    610                 615                 620                 


Phe His Tyr Asp Arg Asn Asn Ile Ala Val Gly Ala Asp Glu Ser Val 
625                 630                 635                 640 


Val Lys Glu Ala His Arg Glu Val Ile Asn Ser Ser Thr Glu Gly Leu 
                645                 650                 655     


Leu Leu Asn Ile Asp Lys Asp Ile Arg Lys Ile Leu Ser Gly Tyr Ile 
            660                 665                 670         


Val Glu Ile Glu Asp Thr Glu Gly Leu Lys Glu Val Ile Asn Asp Arg 
        675                 680                 685             


Tyr Asp Met Leu Asn Ile Ser Ser Leu Arg Gln Asp Gly Lys Thr Phe 
    690                 695                 700                 


Ile Asp Phe Lys Lys Tyr Asn Asp Lys Leu Pro Leu Tyr Ile Ser Asn 
705                 710                 715                 720 


Pro Asn Tyr Lys Val Asn Val Tyr Ala Val Thr Lys Glu Asn Thr Ile 
                725                 730                 735     


Ile Asn Pro Ser Glu Asn Gly Asp Thr Ser Thr Asn Gly Ile Lys Lys 
            740                 745                 750         


Ile Leu Ile Phe Ser Lys Lys Gly Tyr Glu Ile Gly 
        755                 760                 


<210>  53
<211>  863
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LHN/A with a C1 Activation Loop

<400>  53

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Val Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys His Lys 
            420                 425                 430         


Ala Ile Asp Gly Arg Ser Leu Tyr Asn Lys Thr Leu Asp Cys Ile Lys 
        435                 440                 445             


Val Asn Asn Trp Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr 
    450                 455                 460                 


Asn Asp Leu Asn Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu 
465                 470                 475                 480 


Ala Ala Glu Glu Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu 
                485                 490                 495     


Thr Phe Asn Phe Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu 
            500                 505                 510         


Ser Ser Asp Ile Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg 
        515                 520                 525             


Phe Pro Asn Gly Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His 
    530                 535                 540                 


Tyr Leu Arg Ala Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu 
545                 550                 555                 560 


Thr Asn Ser Val Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr 
                565                 570                 575     


Phe Phe Ser Ser Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala 
            580                 585                 590         


Ala Met Phe Leu Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp 
        595                 600                 605             


Glu Thr Ser Glu Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile 
    610                 615                 620                 


Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr 
625                 630                 635                 640 


Lys Asp Asp Phe Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu 
                645                 650                 655     


Leu Glu Phe Ile Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala 
            660                 665                 670         


Leu Val Ser Tyr Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp 
        675                 680                 685             


Asn Ala Leu Ser Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr 
    690                 695                 700                 


Ile Val Thr Asn Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile 
705                 710                 715                 720 


Arg Lys Lys Met Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys 
                725                 730                 735     


Ala Ile Ile Asn Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn 
            740                 745                 750         


Asn Ile Asn Phe Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser 
        755                 760                 765             


Ile Asn Lys Ala Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser 
    770                 775                 780                 


Val Ser Tyr Leu Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu 
785                 790                 795                 800 


Glu Asp Phe Asp Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr 
                805                 810                 815     


Asp Asn Arg Gly Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys 
            820                 825                 830         


Val Asn Asn Thr Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr 
        835                 840                 845             


Val Asp Asn Gln Arg Leu Leu Ser Thr Phe Thr Glu Tyr Ile Lys 
    850                 855                 860             


<210>  54
<211>  865
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LHN/B with a C1 Activation Loop

<400>  54

Met Pro Val Thr Ile Asn Asn Phe Asn Tyr Asn Asp Pro Ile Asp Asn 
1               5                   10                  15      


Asn Asn Ile Ile Met Met Glu Pro Pro Phe Ala Arg Gly Thr Gly Arg 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Tyr Thr Phe Gly Tyr Lys Pro Glu Asp Phe Asn Lys Ser Ser Gly 
    50                  55                  60                  


Ile Phe Asn Arg Asp Val Cys Glu Tyr Tyr Asp Pro Asp Tyr Leu Asn 
65                  70                  75                  80  


Thr Asn Asp Lys Lys Asn Ile Phe Leu Gln Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Lys Ser Lys Pro Leu Gly Glu Lys Leu Leu Glu Met Ile 
            100                 105                 110         


Ile Asn Gly Ile Pro Tyr Leu Gly Asp Arg Arg Val Pro Leu Glu Glu 
        115                 120                 125             


Phe Asn Thr Asn Ile Ala Ser Val Thr Val Asn Lys Leu Ile Ser Asn 
    130                 135                 140                 


Pro Gly Glu Val Glu Arg Lys Lys Gly Ile Phe Ala Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Asn Glu Asn Glu Thr Ile Asp Ile Gly 
                165                 170                 175     


Ile Gln Asn His Phe Ala Ser Arg Glu Gly Phe Gly Gly Ile Met Gln 
            180                 185                 190         


Met Lys Phe Cys Pro Glu Tyr Val Ser Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Gly Ala Ser Ile Phe Asn Arg Arg Gly Tyr Phe Ser Asp Pro 
    210                 215                 220                 


Ala Leu Ile Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Val Asp Asp Leu Pro Ile Val Pro Asn Glu Lys Lys Phe 
                245                 250                 255     


Phe Met Gln Ser Thr Asp Ala Ile Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly Gln Asp Pro Ser Ile Ile Thr Pro Ser Thr Asp Lys Ser Ile 
        275                 280                 285             


Tyr Asp Lys Val Leu Gln Asn Phe Arg Gly Ile Val Asp Arg Leu Asn 
    290                 295                 300                 


Lys Val Leu Val Cys Ile Ser Asp Pro Asn Ile Asn Ile Asn Ile Tyr 
305                 310                 315                 320 


Lys Asn Lys Phe Lys Asp Lys Tyr Lys Phe Val Glu Asp Ser Glu Gly 
                325                 330                 335     


Lys Tyr Ser Ile Asp Val Glu Ser Phe Asp Lys Leu Tyr Lys Ser Leu 
            340                 345                 350         


Met Phe Gly Phe Thr Glu Thr Asn Ile Ala Glu Asn Tyr Lys Ile Lys 
        355                 360                 365             


Thr Arg Ala Ser Tyr Phe Ser Asp Ser Leu Pro Pro Val Lys Ile Lys 
    370                 375                 380                 


Asn Leu Leu Asp Asn Glu Ile Tyr Thr Ile Glu Glu Gly Phe Asn Ile 
385                 390                 395                 400 


Ser Asp Lys Asp Met Glu Lys Glu Tyr Arg Gly Gln Asn Lys Ala Ile 
                405                 410                 415     


Asn Lys Gln Ala Tyr Glu Glu Ile Ser Lys Glu His Leu Ala Val Tyr 
            420                 425                 430         


Lys Ile Gln Met Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn 
        435                 440                 445             


Lys Thr Leu Asp Cys Ile Asp Val Asp Asn Glu Asp Leu Phe Phe Ile 
    450                 455                 460                 


Ala Asp Lys Asn Ser Phe Ser Asp Asp Leu Ser Lys Asn Glu Arg Ile 
465                 470                 475                 480 


Glu Tyr Asn Thr Gln Ser Asn Tyr Ile Glu Asn Asp Phe Pro Ile Asn 
                485                 490                 495     


Glu Leu Ile Leu Asp Thr Asp Leu Ile Ser Lys Ile Glu Leu Pro Ser 
            500                 505                 510         


Glu Asn Thr Glu Ser Leu Thr Asp Phe Asn Val Asp Val Pro Val Tyr 
        515                 520                 525             


Glu Lys Gln Pro Ala Ile Lys Lys Ile Phe Thr Asp Glu Asn Thr Ile 
    530                 535                 540                 


Phe Gln Tyr Leu Tyr Ser Gln Thr Phe Pro Leu Asp Ile Arg Asp Ile 
545                 550                 555                 560 


Ser Leu Thr Ser Ser Phe Asp Asp Ala Leu Leu Phe Ser Asn Lys Val 
                565                 570                 575     


Tyr Ser Phe Phe Ser Met Asp Tyr Ile Lys Thr Ala Asn Lys Val Val 
            580                 585                 590         


Glu Ala Gly Leu Phe Ala Gly Trp Val Lys Gln Ile Val Asn Asp Phe 
        595                 600                 605             


Val Ile Glu Ala Asn Lys Ser Asn Thr Met Asp Lys Ile Ala Asp Ile 
    610                 615                 620                 


Ser Leu Ile Val Pro Tyr Ile Gly Leu Ala Leu Asn Val Gly Asn Glu 
625                 630                 635                 640 


Thr Ala Lys Gly Asn Phe Glu Asn Ala Phe Glu Ile Ala Gly Ala Ser 
                645                 650                 655     


Ile Leu Leu Glu Phe Ile Pro Glu Leu Leu Ile Pro Val Val Gly Ala 
            660                 665                 670         


Phe Leu Leu Glu Ser Tyr Ile Asp Asn Lys Asn Lys Ile Ile Lys Thr 
        675                 680                 685             


Ile Asp Asn Ala Leu Thr Lys Arg Asn Glu Lys Trp Ser Asp Met Tyr 
    690                 695                 700                 


Gly Leu Ile Val Ala Gln Trp Leu Ser Thr Val Asn Thr Gln Phe Tyr 
705                 710                 715                 720 


Thr Ile Lys Glu Gly Met Tyr Lys Ala Leu Asn Tyr Gln Ala Gln Ala 
                725                 730                 735     


Leu Glu Glu Ile Ile Lys Tyr Arg Tyr Asn Ile Tyr Ser Glu Lys Glu 
            740                 745                 750         


Lys Ser Asn Ile Asn Ile Asp Phe Asn Asp Ile Asn Ser Lys Leu Asn 
        755                 760                 765             


Glu Gly Ile Asn Gln Ala Ile Asp Asn Ile Asn Asn Phe Ile Asn Gly 
    770                 775                 780                 


Cys Ser Val Ser Tyr Leu Met Lys Lys Met Ile Pro Leu Ala Val Glu 
785                 790                 795                 800 


Lys Leu Leu Asp Phe Asp Asn Thr Leu Lys Lys Asn Leu Leu Asn Tyr 
                805                 810                 815     


Ile Asp Glu Asn Lys Leu Tyr Leu Ile Gly Ser Ala Glu Tyr Glu Lys 
            820                 825                 830         


Ser Lys Val Asn Lys Tyr Leu Lys Thr Ile Met Pro Phe Asp Leu Ser 
        835                 840                 845             


Ile Tyr Thr Asn Asp Thr Ile Leu Ile Glu Met Phe Asn Lys Tyr Asn 
    850                 855                 860                 


Ser 
865 


<210>  55
<211>  865
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LHN/D with a C1 Activation Loop

<400>  55

Met Thr Trp Pro Val Lys Asp Phe Asn Tyr Ser Asp Pro Val Asn Asp 
1               5                   10                  15      


Asn Asp Ile Leu Tyr Leu Arg Ile Pro Gln Asn Lys Leu Ile Thr Thr 
            20                  25                  30          


Pro Val Lys Ala Phe Met Ile Thr Gln Asn Ile Trp Val Ile Pro Glu 
        35                  40                  45              


Arg Phe Ser Ser Asp Thr Asn Pro Ser Leu Ser Lys Pro Pro Arg Pro 
    50                  55                  60                  


Thr Ser Lys Tyr Gln Ser Tyr Tyr Asp Pro Ser Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Glu Gln Lys Asp Thr Phe Leu Lys Gly Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Glu Arg Asp Ile Gly Lys Lys Leu Ile Asn Tyr Leu Val Val 
            100                 105                 110         


Gly Ser Pro Phe Met Gly Asp Ser Ser Thr Pro Glu Asp Thr Phe Asp 
        115                 120                 125             


Phe Thr Arg His Thr Thr Asn Ile Ala Val Glu Lys Phe Glu Asn Gly 
    130                 135                 140                 


Ser Trp Lys Val Thr Asn Ile Ile Thr Pro Ser Val Leu Ile Phe Gly 
145                 150                 155                 160 


Pro Leu Pro Asn Ile Leu Asp Tyr Thr Ala Ser Leu Thr Leu Gln Gly 
                165                 170                 175     


Gln Gln Ser Asn Pro Ser Phe Glu Gly Phe Gly Thr Leu Ser Ile Leu 
            180                 185                 190         


Lys Val Ala Pro Glu Phe Leu Leu Thr Phe Ser Asp Val Thr Ser Asn 
        195                 200                 205             


Gln Ser Ser Ala Val Leu Gly Lys Ser Ile Phe Cys Met Asp Pro Val 
    210                 215                 220                 


Ile Ala Leu Met His Glu Leu Thr His Ser Leu His Gln Leu Tyr Gly 
225                 230                 235                 240 


Ile Asn Ile Pro Ser Asp Lys Arg Ile Arg Pro Gln Val Ser Glu Gly 
                245                 250                 255     


Phe Phe Ser Gln Asp Gly Pro Asn Val Gln Phe Glu Glu Leu Tyr Thr 
            260                 265                 270         


Phe Gly Gly Leu Asp Val Glu Ile Ile Pro Gln Ile Glu Arg Ser Gln 
        275                 280                 285             


Leu Arg Glu Lys Ala Leu Gly His Tyr Lys Asp Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Asn Ile Asn Lys Thr Ile Pro Ser Ser Trp Ile Ser Asn Ile Asp 
305                 310                 315                 320 


Lys Tyr Lys Lys Ile Phe Ser Glu Lys Tyr Asn Phe Asp Lys Asp Asn 
                325                 330                 335     


Thr Gly Asn Phe Val Val Asn Ile Asp Lys Phe Asn Ser Leu Tyr Ser 
            340                 345                 350         


Asp Leu Thr Asn Val Met Ser Glu Val Val Tyr Ser Ser Gln Tyr Asn 
        355                 360                 365             


Val Lys Asn Arg Thr His Tyr Phe Ser Arg His Tyr Leu Pro Val Phe 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Ile Tyr Thr Ile Arg Asp Gly Phe Asn 
385                 390                 395                 400 


Leu Thr Asn Lys Gly Phe Asn Ile Glu Asn Ser Gly Gln Asn Ile Glu 
                405                 410                 415     


Arg Asn Pro Ala Leu Gln Lys Leu Ser Ser Glu Ser Val Val Asp Leu 
            420                 425                 430         


Phe Thr Lys Val Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn 
        435                 440                 445             


Lys Thr Leu Asp Cys Ile Lys Val Lys Asn Asn Arg Leu Pro Tyr Val 
    450                 455                 460                 


Ala Asp Lys Asp Ser Ile Ser Gln Glu Ile Phe Glu Asn Lys Ile Ile 
465                 470                 475                 480 


Thr Asp Glu Thr Asn Val Gln Asn Tyr Ser Asp Lys Phe Ser Leu Asp 
                485                 490                 495     


Glu Ser Ile Leu Asp Gly Gln Val Pro Ile Asn Pro Glu Ile Val Asp 
            500                 505                 510         


Pro Leu Leu Pro Asn Val Asn Met Glu Pro Leu Asn Leu Pro Gly Glu 
        515                 520                 525             


Glu Ile Val Phe Tyr Asp Asp Ile Thr Lys Tyr Val Asp Tyr Leu Asn 
    530                 535                 540                 


Ser Tyr Tyr Tyr Leu Glu Ser Gln Lys Leu Ser Asn Asn Val Glu Asn 
545                 550                 555                 560 


Ile Thr Leu Thr Thr Ser Val Glu Glu Ala Leu Gly Tyr Ser Asn Lys 
                565                 570                 575     


Ile Tyr Thr Phe Leu Pro Ser Leu Ala Glu Lys Val Asn Lys Gly Val 
            580                 585                 590         


Gln Ala Gly Leu Phe Leu Asn Trp Ala Asn Glu Val Val Glu Asp Phe 
        595                 600                 605             


Thr Thr Asn Ile Met Lys Lys Asp Thr Leu Asp Lys Ile Ser Asp Val 
    610                 615                 620                 


Ser Val Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Ser 
625                 630                 635                 640 


Ala Leu Arg Gly Asn Phe Asn Gln Ala Phe Ala Thr Ala Gly Val Ala 
                645                 650                 655     


Phe Leu Leu Glu Gly Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly Val 
            660                 665                 670         


Phe Thr Phe Tyr Ser Ser Ile Gln Glu Arg Glu Lys Ile Ile Lys Thr 
        675                 680                 685             


Ile Glu Asn Cys Leu Glu Gln Arg Val Lys Arg Trp Lys Asp Ser Tyr 
    690                 695                 700                 


Gln Trp Met Val Ser Asn Trp Leu Ser Arg Ile Thr Thr Gln Phe Asn 
705                 710                 715                 720 


His Ile Asn Tyr Gln Met Tyr Asp Ser Leu Ser Tyr Gln Ala Asp Ala 
                725                 730                 735     


Ile Lys Ala Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser Asp 
            740                 745                 750         


Lys Glu Asn Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu Asp 
        755                 760                 765             


Val Lys Ile Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg Glu 
    770                 775                 780                 


Cys Ser Val Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile Asp 
785                 790                 795                 800 


Glu Leu Asn Lys Phe Asp Leu Arg Thr Lys Thr Glu Leu Ile Asn Leu 
                805                 810                 815     


Ile Asp Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Arg Leu Lys 
            820                 825                 830         


Ala Lys Val Asn Glu Ser Phe Glu Asn Thr Met Pro Phe Asn Ile Phe 
        835                 840                 845             


Ser Tyr Thr Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr Phe 
    850                 855                 860                 


Asn 
865 


<210>  56
<211>  847
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LHN/E with a C1 Activation Loop

<400>  56

Met Pro Lys Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp Arg 
1               5                   10                  15      


Thr Ile Leu Tyr Ile Lys Pro Gly Gly Cys Gln Glu Phe Tyr Lys Ser 
            20                  25                  30          


Phe Asn Ile Met Lys Asn Ile Trp Ile Ile Pro Glu Arg Asn Val Ile 
        35                  40                  45              


Gly Thr Thr Pro Gln Asp Phe His Pro Pro Thr Ser Leu Lys Asn Gly 
    50                  55                  60                  


Asp Ser Ser Tyr Tyr Asp Pro Asn Tyr Leu Gln Ser Asp Glu Glu Lys 
65                  70                  75                  80  


Asp Arg Phe Leu Lys Ile Val Thr Lys Ile Phe Asn Arg Ile Asn Asn 
                85                  90                  95      


Asn Leu Ser Gly Gly Ile Leu Leu Glu Glu Leu Ser Lys Ala Asn Pro 
            100                 105                 110         


Tyr Leu Gly Asn Asp Asn Thr Pro Asp Asn Gln Phe His Ile Gly Asp 
        115                 120                 125             


Ala Ser Ala Val Glu Ile Lys Phe Ser Asn Gly Ser Gln Asp Ile Leu 
    130                 135                 140                 


Leu Pro Asn Val Ile Ile Met Gly Ala Glu Pro Asp Leu Phe Glu Thr 
145                 150                 155                 160 


Asn Ser Ser Asn Ile Ser Leu Arg Asn Asn Tyr Met Pro Ser Asn His 
                165                 170                 175     


Arg Phe Gly Ser Ile Ala Ile Val Thr Phe Ser Pro Glu Tyr Ser Phe 
            180                 185                 190         


Arg Phe Asn Asp Asn Cys Met Asn Glu Phe Ile Gln Asp Pro Ala Leu 
        195                 200                 205             


Thr Leu Met His Glu Leu Ile His Ser Leu His Gly Leu Tyr Gly Ala 
    210                 215                 220                 


Lys Gly Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn Pro Leu 
225                 230                 235                 240 


Ile Thr Asn Ile Arg Gly Thr Asn Ile Glu Glu Phe Leu Thr Phe Gly 
                245                 250                 255     


Gly Thr Asp Leu Asn Ile Ile Thr Ser Ala Gln Ser Asn Asp Ile Tyr 
            260                 265                 270         


Thr Asn Leu Leu Ala Asp Tyr Lys Lys Ile Ala Ser Lys Leu Ser Lys 
        275                 280                 285             


Val Gln Val Ser Asn Pro Leu Leu Asn Pro Tyr Lys Asp Val Phe Glu 
    290                 295                 300                 


Ala Lys Tyr Gly Leu Asp Lys Asp Ala Ser Gly Ile Tyr Ser Val Asn 
305                 310                 315                 320 


Ile Asn Lys Phe Asn Asp Ile Phe Lys Lys Leu Tyr Ser Phe Thr Glu 
                325                 330                 335     


Phe Asp Leu Arg Thr Lys Phe Gln Val Lys Cys Arg Gln Thr Tyr Ile 
            340                 345                 350         


Gly Gln Tyr Lys Tyr Phe Lys Leu Ser Asn Leu Leu Asn Asp Ser Ile 
        355                 360                 365             


Tyr Asn Ile Ser Glu Gly Tyr Asn Ile Asn Asn Leu Lys Val Asn Phe 
    370                 375                 380                 


Arg Gly Gln Asn Ala Asn Leu Asn Pro Arg Ile Ile Thr Pro Ile Thr 
385                 390                 395                 400 


Gly Arg Gly Leu Val Lys Lys Ile Ile Arg Phe Cys His Lys Ala Ile 
                405                 410                 415     


Asp Gly Arg Ser Leu Tyr Asn Lys Thr Leu Asp Cys Ile Glu Ile Asn 
            420                 425                 430         


Asn Gly Glu Leu Phe Phe Val Ala Ser Glu Asn Ser Tyr Asn Asp Asp 
        435                 440                 445             


Asn Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Val Thr Ser Asn Asn 
    450                 455                 460                 


Asn Tyr Glu Asn Asp Leu Asp Gln Val Ile Leu Asn Phe Asn Ser Glu 
465                 470                 475                 480 


Ser Ala Pro Gly Leu Ser Asp Glu Lys Leu Asn Leu Thr Ile Gln Asn 
                485                 490                 495     


Asp Ala Tyr Ile Pro Lys Tyr Asp Ser Asn Gly Thr Ser Asp Ile Glu 
            500                 505                 510         


Gln His Asp Val Asn Glu Leu Asn Val Phe Phe Tyr Leu Asp Ala Gln 
        515                 520                 525             


Lys Val Pro Glu Gly Glu Asn Asn Val Asn Leu Thr Ser Ser Ile Asp 
    530                 535                 540                 


Thr Ala Leu Leu Glu Gln Pro Lys Ile Tyr Thr Phe Phe Ser Ser Glu 
545                 550                 555                 560 


Phe Ile Asn Asn Val Asn Lys Pro Val Gln Ala Ala Leu Phe Val Ser 
                565                 570                 575     


Trp Ile Gln Gln Val Leu Val Asp Phe Thr Thr Glu Ala Asn Gln Lys 
            580                 585                 590         


Ser Thr Val Asp Lys Ile Ala Asp Ile Ser Ile Val Val Pro Tyr Ile 
        595                 600                 605             


Gly Leu Ala Leu Asn Ile Gly Asn Glu Ala Gln Lys Gly Asn Phe Lys 
    610                 615                 620                 


Asp Ala Leu Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Glu Pro 
625                 630                 635                 640 


Glu Leu Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe Leu 
                645                 650                 655     


Gly Ser Ser Asp Asn Lys Asn Lys Val Ile Lys Ala Ile Asn Asn Ala 
            660                 665                 670         


Leu Lys Glu Arg Asp Glu Lys Trp Lys Glu Val Tyr Ser Phe Ile Val 
        675                 680                 685             


Ser Asn Trp Met Thr Lys Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu 
    690                 695                 700                 


Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asn Ala Ile Lys Thr Ile 
705                 710                 715                 720 


Ile Glu Ser Lys Tyr Asn Ser Tyr Thr Leu Glu Glu Lys Asn Glu Leu 
                725                 730                 735     


Thr Asn Lys Tyr Asp Ile Lys Gln Ile Glu Asn Glu Leu Asn Gln Lys 
            740                 745                 750         


Val Ser Ile Ala Met Asn Asn Ile Asp Arg Phe Leu Thr Glu Ser Ser 
        755                 760                 765             


Ile Ser Tyr Leu Met Lys Ile Ile Asn Glu Val Lys Ile Asn Lys Leu 
    770                 775                 780                 


Arg Glu Tyr Asp Glu Asn Val Lys Thr Tyr Leu Leu Asn Tyr Ile Ile 
785                 790                 795                 800 


Gln His Gly Ser Ile Leu Gly Glu Ser Gln Gln Glu Leu Asn Ser Met 
                805                 810                 815     


Val Thr Asp Thr Leu Asn Asn Ser Ile Pro Phe Lys Leu Ser Ser Tyr 
            820                 825                 830         


Thr Asp Asp Lys Ile Leu Ile Ser Tyr Phe Asn Lys Phe Phe Lys 
        835                 840                 845         


<210>  57
<211>  864
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LHN/F with a C1 Activation Loop

<400>  57

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp 
1               5                   10                  15      


Asp Thr Ile Leu Tyr Met Gln Ile Pro Tyr Glu Glu Lys Ser Lys Lys 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Glu Ile Met Arg Asn Val Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Asn Thr Ile Gly Thr Asp Pro Ser Asp Phe Asp Pro Pro Ala Ser 
    50                  55                  60                  


Leu Glu Asn Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr 
65                  70                  75                  80  


Asp Ala Glu Lys Asp Arg Tyr Leu Lys Thr Thr Ile Lys Leu Phe Lys 
                85                  90                  95      


Arg Ile Asn Ser Asn Pro Ala Gly Glu Val Leu Leu Gln Glu Ile Ser 
            100                 105                 110         


Tyr Ala Lys Pro Tyr Leu Gly Asn Glu His Thr Pro Ile Asn Glu Phe 
        115                 120                 125             


His Pro Val Thr Arg Thr Thr Ser Val Asn Ile Lys Ser Ser Thr Asn 
    130                 135                 140                 


Val Lys Ser Ser Ile Ile Leu Asn Leu Leu Val Leu Gly Ala Gly Pro 
145                 150                 155                 160 


Asp Ile Phe Glu Asn Ser Ser Tyr Pro Val Arg Lys Leu Met Asp Ser 
                165                 170                 175     


Gly Gly Val Tyr Asp Pro Ser Asn Asp Gly Phe Gly Ser Ile Asn Ile 
            180                 185                 190         


Val Thr Phe Ser Pro Glu Tyr Glu Tyr Thr Phe Asn Asp Ile Ser Gly 
        195                 200                 205             


Gly Tyr Asn Ser Ser Thr Glu Ser Phe Ile Ala Asp Pro Ala Ile Ser 
    210                 215                 220                 


Leu Ala His Glu Leu Ile His Ala Leu His Gly Leu Tyr Gly Ala Arg 
225                 230                 235                 240 


Gly Val Thr Tyr Lys Glu Thr Ile Lys Val Lys Gln Ala Pro Leu Met 
                245                 250                 255     


Ile Ala Glu Lys Pro Ile Arg Leu Glu Glu Phe Leu Thr Phe Gly Gly 
            260                 265                 270         


Gln Asp Leu Asn Ile Ile Thr Ser Ala Met Lys Glu Lys Ile Tyr Asn 
        275                 280                 285             


Asn Leu Leu Ala Asn Tyr Glu Lys Ile Ala Thr Arg Leu Ser Arg Val 
    290                 295                 300                 


Asn Ser Ala Pro Pro Glu Tyr Asp Ile Asn Glu Tyr Lys Asp Tyr Phe 
305                 310                 315                 320 


Gln Trp Lys Tyr Gly Leu Asp Lys Asn Ala Asp Gly Ser Tyr Thr Val 
                325                 330                 335     


Asn Glu Asn Lys Phe Asn Glu Ile Tyr Lys Lys Leu Tyr Ser Phe Thr 
            340                 345                 350         


Glu Ile Asp Leu Ala Asn Lys Phe Lys Val Lys Cys Arg Asn Thr Tyr 
        355                 360                 365             


Phe Ile Lys Tyr Gly Phe Leu Lys Val Pro Asn Leu Leu Asp Asp Asp 
    370                 375                 380                 


Ile Tyr Thr Val Ser Glu Gly Phe Asn Ile Gly Asn Leu Ala Val Asn 
385                 390                 395                 400 


Asn Arg Gly Gln Asn Ile Lys Leu Asn Pro Lys Ile Ile Asp Ser Ile 
                405                 410                 415     


Pro Asp Lys Gly Leu Val Glu Lys Ile Val Lys Phe Cys His Lys Ala 
            420                 425                 430         


Ile Asp Gly Arg Ser Leu Tyr Asn Lys Thr Leu Asp Cys Ile Arg Val 
        435                 440                 445             


Asn Asn Arg Glu Leu Phe Phe Val Ala Ser Glu Ser Ser Tyr Asn Glu 
    450                 455                 460                 


Asn Asp Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Thr Asn Leu Asn 
465                 470                 475                 480 


Asn Asn Tyr Arg Asn Asn Leu Asp Glu Val Ile Leu Asp Tyr Asn Ser 
                485                 490                 495     


Glu Thr Ile Pro Gln Ile Ser Asn Gln Thr Leu Asn Thr Leu Val Gln 
            500                 505                 510         


Asp Asp Ser Tyr Val Pro Arg Tyr Asp Ser Asn Gly Thr Ser Glu Ile 
        515                 520                 525             


Glu Glu His Asn Val Val Asp Leu Asn Val Phe Phe Tyr Leu His Ala 
    530                 535                 540                 


Gln Lys Val Pro Glu Gly Glu Thr Asn Ile Ser Leu Thr Ser Ser Ile 
545                 550                 555                 560 


Asp Thr Ala Leu Ser Glu Glu Ser Gln Val Tyr Thr Phe Phe Ser Ser 
                565                 570                 575     


Glu Phe Ile Asn Thr Ile Asn Lys Pro Val His Ala Ala Leu Phe Ile 
            580                 585                 590         


Ser Trp Ile Asn Gln Val Ile Arg Asp Phe Thr Thr Glu Ala Thr Gln 
        595                 600                 605             


Lys Ser Thr Phe Asp Lys Ile Ala Asp Ile Ser Leu Val Val Pro Tyr 
    610                 615                 620                 


Val Gly Leu Ala Leu Asn Ile Gly Asn Glu Val Gln Lys Glu Asn Phe 
625                 630                 635                 640 


Lys Glu Ala Phe Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Val 
                645                 650                 655     


Pro Glu Leu Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe 
            660                 665                 670         


Ile Gly Ser Ser Glu Asn Lys Asn Lys Ile Ile Lys Ala Ile Asn Asn 
        675                 680                 685             


Ser Leu Met Glu Arg Glu Thr Lys Trp Lys Glu Ile Tyr Ser Trp Ile 
    690                 695                 700                 


Val Ser Asn Trp Leu Thr Arg Ile Asn Thr Gln Phe Asn Lys Arg Lys 
705                 710                 715                 720 


Glu Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asp Ala Ile Lys Thr 
                725                 730                 735     


Val Ile Glu Tyr Lys Tyr Asn Asn Tyr Thr Ser Asp Glu Arg Asn Arg 
            740                 745                 750         


Leu Glu Ser Glu Tyr Asn Ile Asn Asn Ile Arg Glu Glu Leu Asn Lys 
        755                 760                 765             


Lys Val Ser Leu Ala Met Glu Asn Ile Glu Arg Phe Ile Thr Glu Ser 
    770                 775                 780                 


Ser Ile Phe Tyr Leu Met Lys Leu Ile Asn Glu Ala Lys Val Ser Lys 
785                 790                 795                 800 


Leu Arg Glu Tyr Asp Glu Gly Val Lys Glu Tyr Leu Leu Asp Tyr Ile 
                805                 810                 815     


Ser Glu His Arg Ser Ile Leu Gly Asn Ser Val Gln Glu Leu Asn Asp 
            820                 825                 830         


Leu Val Thr Ser Thr Leu Asn Asn Ser Ile Pro Phe Glu Leu Ser Ser 
        835                 840                 845             


Tyr Thr Asn Asp Lys Ile Leu Ile Leu Tyr Phe Asn Lys Leu Tyr Lys 
    850                 855                 860                 


<210>  58
<211>  865
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LHN/G with a C1 Activation Loop


<220>
<221>  SITE
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  58

Met Pro Val Asn Ile Lys Xaa Phe Asn Tyr Asn Asp Pro Ile Asn Asn 
1               5                   10                  15      


Asp Asp Ile Ile Met Met Glu Pro Phe Asn Asp Pro Gly Pro Gly Thr 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Arg Ile Ile Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Phe Thr Tyr Gly Phe Gln Pro Asp Gln Phe Asn Ala Ser Thr Gly 
    50                  55                  60                  


Val Phe Ser Lys Asp Val Tyr Glu Tyr Tyr Asp Pro Thr Tyr Leu Lys 
65                  70                  75                  80  


Thr Asp Ala Glu Lys Asp Lys Phe Leu Lys Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Asn Ser Lys Pro Ser Gly Gln Arg Leu Leu Asp Met Ile 
            100                 105                 110         


Val Asp Ala Ile Pro Tyr Leu Gly Asn Ala Ser Thr Pro Pro Asp Lys 
        115                 120                 125             


Phe Ala Ala Asn Val Ala Asn Val Ser Ile Asn Lys Lys Ile Ile Gln 
    130                 135                 140                 


Pro Gly Ala Glu Asp Gln Ile Lys Gly Leu Met Thr Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Ser Asp Asn Phe Thr Asp Ser Met Ile 
                165                 170                 175     


Met Asn Gly His Ser Pro Ile Ser Glu Gly Phe Gly Ala Arg Met Met 
            180                 185                 190         


Ile Arg Phe Cys Pro Ser Cys Leu Asn Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Asp Thr Ser Ile Phe Ser Arg Arg Ala Tyr Phe Ala Asp Pro 
    210                 215                 220                 


Ala Leu Thr Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Ile Ser Asn Leu Pro Ile Thr Pro Asn Thr Lys Glu Phe 
                245                 250                 255     


Phe Met Gln His Ser Asp Pro Val Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly His Asp Pro Ser Val Ile Ser Pro Ser Thr Asp Met Asn Ile 
        275                 280                 285             


Tyr Asn Lys Ala Leu Gln Asn Phe Gln Asp Ile Ala Asn Arg Leu Asn 
    290                 295                 300                 


Ile Val Ser Ser Ala Gln Gly Ser Gly Ile Asp Ile Ser Leu Tyr Lys 
305                 310                 315                 320 


Gln Ile Tyr Lys Asn Lys Tyr Asp Phe Val Glu Asp Pro Asn Gly Lys 
                325                 330                 335     


Tyr Ser Val Asp Lys Asp Lys Phe Asp Lys Leu Tyr Lys Ala Leu Met 
            340                 345                 350         


Phe Gly Phe Thr Glu Thr Asn Leu Ala Gly Glu Tyr Gly Ile Lys Thr 
        355                 360                 365             


Arg Tyr Ser Tyr Phe Ser Glu Tyr Leu Pro Pro Ile Lys Thr Glu Lys 
    370                 375                 380                 


Leu Leu Asp Asn Thr Ile Tyr Thr Gln Asn Glu Gly Phe Asn Ile Ala 
385                 390                 395                 400 


Ser Lys Asn Leu Lys Thr Glu Phe Asn Gly Gln Asn Lys Ala Val Asn 
                405                 410                 415     


Lys Glu Ala Tyr Glu Glu Ile Ser Leu Glu His Leu Val Ile Tyr Arg 
            420                 425                 430         


Ile Ala Met Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn Lys 
        435                 440                 445             


Thr Leu Asp Cys Ile Ile Val Asn Asn Glu Asp Leu Phe Phe Ile Ala 
    450                 455                 460                 


Asn Lys Asp Ser Phe Ser Lys Asp Leu Ala Lys Ala Glu Thr Ile Ala 
465                 470                 475                 480 


Tyr Asn Thr Gln Asn Asn Thr Ile Glu Asn Asn Phe Ser Ile Asp Gln 
                485                 490                 495     


Leu Ile Leu Asp Asn Asp Leu Ser Ser Gly Ile Asp Leu Pro Asn Glu 
            500                 505                 510         


Asn Thr Glu Pro Phe Thr Asn Phe Asp Asp Ile Asp Ile Pro Val Tyr 
        515                 520                 525             


Ile Lys Gln Ser Ala Leu Lys Lys Ile Phe Val Asp Gly Asp Ser Leu 
    530                 535                 540                 


Phe Glu Tyr Leu His Ala Gln Thr Phe Pro Ser Asn Ile Glu Asn Leu 
545                 550                 555                 560 


Gln Leu Thr Asn Ser Leu Asn Asp Ala Leu Arg Asn Asn Asn Lys Val 
                565                 570                 575     


Tyr Thr Phe Phe Ser Thr Asn Leu Val Glu Lys Ala Asn Thr Val Val 
            580                 585                 590         


Gly Ala Ser Leu Phe Val Asn Trp Val Lys Gly Val Ile Asp Asp Phe 
        595                 600                 605             


Thr Ser Glu Ser Thr Gln Lys Ser Thr Ile Asp Lys Val Ser Asp Val 
    610                 615                 620                 


Ser Ile Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Val Gly Asn Glu 
625                 630                 635                 640 


Thr Ala Lys Glu Asn Phe Lys Asn Ala Phe Glu Ile Gly Gly Ala Ala 
                645                 650                 655     


Ile Leu Met Glu Phe Ile Pro Glu Leu Ile Val Pro Ile Val Gly Phe 
            660                 665                 670         


Phe Thr Leu Glu Ser Tyr Val Gly Asn Lys Gly His Ile Ile Met Thr 
        675                 680                 685             


Ile Ser Asn Ala Leu Lys Lys Arg Asp Gln Lys Trp Thr Asp Met Tyr 
    690                 695                 700                 


Gly Leu Ile Val Ser Gln Trp Leu Ser Thr Val Asn Thr Gln Phe Tyr 
705                 710                 715                 720 


Thr Ile Lys Glu Arg Met Tyr Asn Ala Leu Asn Asn Gln Ser Gln Ala 
                725                 730                 735     


Ile Glu Lys Ile Ile Glu Asp Gln Tyr Asn Arg Tyr Ser Glu Glu Asp 
            740                 745                 750         


Lys Met Asn Ile Asn Ile Asp Phe Asn Asp Ile Asp Phe Lys Leu Asn 
        755                 760                 765             


Gln Ser Ile Asn Leu Ala Ile Asn Asn Ile Asp Asp Phe Ile Asn Gln 
    770                 775                 780                 


Cys Ser Ile Ser Tyr Leu Met Asn Arg Met Ile Pro Leu Ala Val Lys 
785                 790                 795                 800 


Lys Leu Lys Asp Phe Asp Asp Asn Leu Lys Arg Asp Leu Leu Glu Tyr 
                805                 810                 815     


Ile Asp Thr Asn Glu Leu Tyr Leu Leu Asp Glu Val Asn Ile Leu Lys 
            820                 825                 830         


Ser Lys Val Asn Arg His Leu Lys Asp Ser Ile Pro Phe Asp Leu Ser 
        835                 840                 845             


Leu Tyr Thr Lys Asp Thr Ile Leu Ile Gln Val Phe Asn Asn Tyr Ile 
    850                 855                 860                 


Ser 
865 


<210>  59
<211>  867
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LHN/TeNT with a C1 Activation Loop

<400>  59

Met Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn 
1               5                   10                  15      


Asp Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser 
    50                  55                  60                  


Leu Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr 
65                  70                  75                  80  


Asp Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn 
                85                  90                  95      


Arg Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile 
            100                 105                 110         


Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe 
        115                 120                 125             


Asp Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro 
    130                 135                 140                 


Ser Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe 
145                 150                 155                 160 


Gly Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu 
                165                 170                 175     


Arg Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser 
            180                 185                 190         


Ile Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn 
        195                 200                 205             


Val Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe 
    210                 215                 220                 


Gln Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His 
225                 230                 235                 240 


Gly Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys 
                245                 250                 255     


Gln Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu 
            260                 265                 270         


Phe Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys 
        275                 280                 285             


Asn Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn 
    290                 295                 300                 


Lys Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp 
305                 310                 315                 320 


Ser Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser 
                325                 330                 335     


Asn Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn 
            340                 345                 350         


Ser Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn 
        355                 360                 365             


Ile Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys 
    370                 375                 380                 


Ile Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe 
385                 390                 395                 400 


Asn Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met 
                405                 410                 415     


Arg Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val 
            420                 425                 430         


Ser Lys Leu Ile Gly Leu Cys His Lys Ala Ile Asp Gly Arg Ser Leu 
        435                 440                 445             


Tyr Asn Lys Thr Leu Asp Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr 
    450                 455                 460                 


Phe Ile Ala Glu Lys Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu 
465                 470                 475                 480 


Ile Val Ser Tyr Asn Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser 
                485                 490                 495     


Leu Asp Lys Ile Ile Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu 
            500                 505                 510         


Pro Asn Asp Arg Thr Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro 
        515                 520                 525             


Glu Tyr Lys Ser Asn Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp 
    530                 535                 540                 


Asp Asn Thr Ile Tyr Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr 
545                 550                 555                 560 


Leu Gln Arg Ile Thr Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn 
                565                 570                 575     


Ser Thr Lys Ile Tyr Ser Tyr Phe Pro Ser Val Ile Ser Lys Val Asn 
            580                 585                 590         


Gln Gly Ala Gln Gly Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile 
        595                 600                 605             


Asp Asp Phe Thr Asn Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile 
    610                 615                 620                 


Ser Asp Val Ser Thr Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile 
625                 630                 635                 640 


Val Lys Gln Gly Tyr Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr 
                645                 650                 655     


Gly Val Val Leu Leu Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val 
            660                 665                 670         


Ile Ala Ala Leu Ser Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile 
        675                 680                 685             


Ile Lys Thr Ile Asp Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile 
    690                 695                 700                 


Glu Val Tyr Lys Leu Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr 
705                 710                 715                 720 


Gln Phe Gln Lys Arg Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln 
                725                 730                 735     


Val Asp Ala Ile Lys Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser 
            740                 745                 750         


Gly Pro Asp Lys Glu Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn 
        755                 760                 765             


Lys Leu Glu Glu Lys Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe 
    770                 775                 780                 


Met Arg Glu Ser Ser Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu 
785                 790                 795                 800 


Ala Lys Lys Gln Leu Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu 
                805                 810                 815     


Met Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu 
            820                 825                 830         


Lys Lys Leu Glu Ser Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro 
        835                 840                 845             


Phe Ser Tyr Ser Lys Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp 
    850                 855                 860                 


Ile Asp Val 
865         


<210>  60
<211>  896
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LHN/X with a C1 Activation Loop

<400>  60

Met Lys Leu Glu Ile Asn Lys Phe Asn Tyr Asn Asp Pro Ile Asp Gly 
1               5                   10                  15      


Ile Asn Val Ile Thr Met Arg Pro Pro Arg His Ser Asp Lys Ile Asn 
            20                  25                  30          


Lys Gly Lys Gly Pro Phe Lys Ala Phe Gln Val Ile Lys Asn Ile Trp 
        35                  40                  45              


Ile Val Pro Glu Arg Tyr Asn Phe Thr Asn Asn Thr Asn Asp Leu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Pro Ile Met Glu Ala Asp Ala Ile Tyr Asn Pro Asn 
65                  70                  75                  80  


Tyr Leu Asn Thr Pro Ser Glu Lys Asp Glu Phe Leu Gln Gly Val Ile 
                85                  90                  95      


Lys Val Leu Glu Arg Ile Lys Ser Lys Pro Glu Gly Glu Lys Leu Leu 
            100                 105                 110         


Glu Leu Ile Ser Ser Ser Ile Pro Leu Pro Leu Val Ser Asn Gly Ala 
        115                 120                 125             


Leu Thr Leu Ser Asp Asn Glu Thr Ile Ala Tyr Gln Glu Asn Asn Asn 
    130                 135                 140                 


Ile Val Ser Asn Leu Gln Ala Asn Leu Val Ile Tyr Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Ile Ala Asn Asn Ala Thr Tyr Gly Leu Tyr Ser Thr Pro Ile Ser 
                165                 170                 175     


Asn Gly Glu Gly Thr Leu Ser Glu Val Ser Phe Ser Pro Phe Tyr Leu 
            180                 185                 190         


Lys Pro Phe Asp Glu Ser Tyr Gly Asn Tyr Arg Ser Leu Val Asn Ile 
        195                 200                 205             


Val Asn Lys Phe Val Lys Arg Glu Phe Ala Pro Asp Pro Ala Ser Thr 
    210                 215                 220                 


Leu Met His Glu Leu Val His Val Thr His Asn Leu Tyr Gly Ile Ser 
225                 230                 235                 240 


Asn Arg Asn Phe Tyr Tyr Asn Phe Asp Thr Gly Lys Ile Glu Thr Ser 
                245                 250                 255     


Arg Gln Gln Asn Ser Leu Ile Phe Glu Glu Leu Leu Thr Phe Gly Gly 
            260                 265                 270         


Ile Asp Ser Lys Ala Ile Ser Ser Leu Ile Ile Lys Lys Ile Ile Glu 
        275                 280                 285             


Thr Ala Lys Asn Asn Tyr Thr Thr Leu Ile Ser Glu Arg Leu Asn Thr 
    290                 295                 300                 


Val Thr Val Glu Asn Asp Leu Leu Lys Tyr Ile Lys Asn Lys Ile Pro 
305                 310                 315                 320 


Val Gln Gly Arg Leu Gly Asn Phe Lys Leu Asp Thr Ala Glu Phe Glu 
                325                 330                 335     


Lys Lys Leu Asn Thr Ile Leu Phe Val Leu Asn Glu Ser Asn Leu Ala 
            340                 345                 350         


Gln Arg Phe Ser Ile Leu Val Arg Lys His Tyr Leu Lys Glu Arg Pro 
        355                 360                 365             


Ile Asp Pro Ile Tyr Val Asn Ile Leu Asp Asp Asn Ser Tyr Ser Thr 
    370                 375                 380                 


Leu Glu Gly Phe Asn Ile Ser Ser Gln Gly Ser Asn Asp Phe Gln Gly 
385                 390                 395                 400 


Gln Leu Leu Glu Ser Ser Tyr Phe Glu Lys Ile Glu Ser Asn Ala Leu 
                405                 410                 415     


Arg Ala Phe Ile Lys Ile Cys His Lys Ala Ile Asp Gly Arg Ser Leu 
            420                 425                 430         


Tyr Asn Lys Thr Leu Asp Cys Ile Glu Val Glu Asn Lys Asp Leu Phe 
        435                 440                 445             


Leu Ile Ser Asn Lys Asp Ser Leu Asn Asp Ile Asn Leu Ser Glu Glu 
    450                 455                 460                 


Lys Ile Lys Pro Glu Thr Thr Val Phe Phe Lys Asp Lys Leu Pro Pro 
465                 470                 475                 480 


Gln Asp Ile Thr Leu Ser Asn Tyr Asp Phe Thr Glu Ala Asn Ser Ile 
                485                 490                 495     


Pro Ser Ile Ser Gln Gln Asn Ile Leu Glu Arg Asn Glu Glu Leu Tyr 
            500                 505                 510         


Glu Pro Ile Arg Asn Ser Leu Phe Glu Ile Lys Thr Ile Tyr Val Asp 
        515                 520                 525             


Lys Leu Thr Thr Phe His Phe Leu Glu Ala Gln Asn Ile Asp Glu Ser 
    530                 535                 540                 


Ile Asp Ser Ser Lys Ile Arg Val Glu Leu Thr Asp Ser Val Asp Glu 
545                 550                 555                 560 


Ala Leu Ser Asn Pro Asn Lys Val Tyr Ser Pro Phe Lys Asn Met Ser 
                565                 570                 575     


Asn Thr Ile Asn Ser Ile Glu Thr Gly Ile Thr Ser Thr Tyr Ile Phe 
            580                 585                 590         


Tyr Gln Trp Leu Arg Ser Ile Val Lys Asp Phe Ser Asp Glu Thr Gly 
        595                 600                 605             


Lys Ile Asp Val Ile Asp Lys Ser Ser Asp Thr Leu Ala Ile Val Pro 
    610                 615                 620                 


Tyr Ile Gly Pro Leu Leu Asn Ile Gly Asn Asp Ile Arg His Gly Asp 
625                 630                 635                 640 


Phe Val Gly Ala Ile Glu Leu Ala Gly Ile Thr Ala Leu Leu Glu Tyr 
                645                 650                 655     


Val Pro Glu Phe Thr Ile Pro Ile Leu Val Gly Leu Glu Val Ile Gly 
            660                 665                 670         


Gly Glu Leu Ala Arg Glu Gln Val Glu Ala Ile Val Asn Asn Ala Leu 
        675                 680                 685             


Asp Lys Arg Asp Gln Lys Trp Ala Glu Val Tyr Asn Ile Thr Lys Ala 
    690                 695                 700                 


Gln Trp Trp Gly Thr Ile His Leu Gln Ile Asn Thr Arg Leu Ala His 
705                 710                 715                 720 


Thr Tyr Lys Ala Leu Ser Arg Gln Ala Asn Ala Ile Lys Met Asn Met 
                725                 730                 735     


Glu Phe Gln Leu Ala Asn Tyr Lys Gly Asn Ile Asp Asp Lys Ala Lys 
            740                 745                 750         


Ile Lys Asn Ala Ile Ser Glu Thr Glu Ile Leu Leu Asn Lys Ser Val 
        755                 760                 765             


Glu Gln Ala Met Lys Asn Thr Glu Lys Phe Met Ile Lys Leu Ser Asn 
    770                 775                 780                 


Ser Tyr Leu Thr Lys Glu Met Ile Pro Lys Val Gln Asp Asn Leu Lys 
785                 790                 795                 800 


Asn Phe Asp Leu Glu Thr Lys Lys Thr Leu Asp Lys Phe Ile Lys Glu 
                805                 810                 815     


Lys Glu Asp Ile Leu Gly Thr Asn Leu Ser Ser Ser Leu Arg Arg Lys 
            820                 825                 830         


Val Ser Ile Arg Leu Asn Lys Asn Ile Ala Phe Asp Ile Asn Asp Ile 
        835                 840                 845             


Pro Phe Ser Glu Phe Asp Asp Leu Ile Asn Gln Tyr Lys Asn Glu Ile 
    850                 855                 860                 


Glu Asp Tyr Glu Val Leu Asn Leu Gly Ala Glu Asp Gly Lys Ile Lys 
865                 870                 875                 880 


Asp Leu Ser Gly Thr Thr Ser Asp Ile Asn Ile Gly Ser Asp Ile Glu 
                885                 890                 895     


<210>  61
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BoNT/DC Activation Loop

<400>  61

Cys Leu Arg Leu Thr Arg Asn Ser Arg Asp Asp Ser Thr Cys 
1               5                   10                  


<210>  62
<211>  25
<212>  PRT
<213>  Clostridium botulinum

<400>  62

Cys Val Arg Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Glu Gly 
1               5                   10                  15      


Tyr Asn Lys Ala Leu Asn Glu Leu Cys 
            20                  25  


<210>  63
<211>  25
<212>  PRT
<213>  Clostridium botulinum

<400>  63

Cys Val Arg Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Glu Gly 
1               5                   10                  15      


Tyr Asn Lys Ala Leu Asn Asp Leu Cys 
            20                  25  


<210>  64
<211>  25
<212>  PRT
<213>  Clostridium botulinum

<400>  64

Cys Val Arg Gly Ile Ile Pro Phe Lys Thr Lys Ser Leu Asp Glu Gly 
1               5                   10                  15      


Tyr Asn Lys Ala Leu Asn Tyr Leu Cys 
            20                  25  


<210>  65
<211>  25
<212>  PRT
<213>  Clostridium botulinum

<400>  65

Cys Val Arg Gly Ile Ile Pro Phe Lys Thr Lys Ser Leu Asp Glu Gly 
1               5                   10                  15      


Tyr Asn Lys Ala Leu Asn Asp Leu Cys 
            20                  25  


<210>  66
<211>  11
<212>  PRT
<213>  Clostridium botulinum

<400>  66

Cys Ser Asn Ser Asn Thr Lys Asn Ser Leu Cys 
1               5                   10      


<210>  67
<211>  15
<212>  PRT
<213>  Clostridium botulinum

<400>  67

Cys Thr Asn Ile Phe Ser Pro Lys Gly Ile Arg Lys Ser Ile Cys 
1               5                   10                  15  


<210>  68
<211>  15
<212>  PRT
<213>  Clostridium botulinum

<400>  68

Cys Lys Asn Ile Val Phe Ser Lys Gly Ile Thr Lys Ser Ile Cys 
1               5                   10                  15  


<210>  69
<211>  15
<212>  PRT
<213>  Clostridium botulinum

<400>  69

Cys Lys Asn Ile Val Phe Ser Lys Gly Ile Arg Lys Ser Ile Cys 
1               5                   10                  15  


<210>  70
<211>  10
<212>  PRT
<213>  Clostridium botulinum

<400>  70

Cys Lys Ser Val Lys Val Pro Gly Ile Cys 
1               5                   10  


<210>  71
<211>  10
<212>  PRT
<213>  Clostridium botulinum

<400>  71

Cys Lys Ser Val Lys Ala Pro Gly Ile Cys 
1               5                   10  


<210>  72
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Enterokinase Cleavage Site

<400>  72

Asp Asp Asp Asp Lys 
1               5   


<210>  73
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Influenza Virus Haemagglutinin Translocation Domain

<400>  73

Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu Gly 
1               5                   10                  15      


Met Ile Asp Gly Trp Tyr Gly 
            20              


<210>  74
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  SITE
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  SITE
<222>  (3)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  74

Xaa Asp Xaa Xaa Xaa Leu Leu 
1               5           


<210>  75
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  SITE
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  SITE
<222>  (3)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  75

Xaa Glu Xaa Xaa Xaa Leu Leu 
1               5           


<210>  76
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  SITE
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  SITE
<222>  (3)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  76

Xaa Glu Xaa Xaa Xaa Ile Leu 
1               5           


<210>  77
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  SITE
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  SITE
<222>  (3)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  77

Xaa Glu Xaa Xaa Xaa Leu Met 
1               5           


<210>  78
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tyrosine-Based Motif


<220>
<221>  SITE
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is a hydrophobic amino acid

<400>  78

Tyr Xaa Xaa Xaa 
1               


<210>  79
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TEV Cleavage Site

<400>  79

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  80
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Thrombin Cleavage Site

<400>  80

Leu Val Pro Arg Gly Ser 
1               5       


<210>  81
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PreScission Cleavage Site

<400>  81

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


