                         SEQUENCE LISTING

<110>  Mab-Legend Biotech Co., Ltd
 
<120>  DUAL TARGETING ANTIGEN BINDING MOLECULE

<130>  FB00032PCT

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  366
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The nucleotide sequence coding for VH of anti-CD20 Fab

<400>  1
gaagtgcagc tggtggagtc tgggggaggc ttggtacagc ctggcaggtc cctgagactc       60

tcctgtgcag cctctggatt cacctttaat gattatgcca tgcactgggt ccggcaagct      120

ccagggaagg gcctggagtg ggtctcaact attagttgga atagtggttc cataggctat      180

gcggactctg tgaagggccg attcaccatc tccagagaca acgccaagaa gtccctgtat      240

ctgcaaatga acagtctgag agctgaggac acggccttgt attactgtgc aaaagatata      300

cagtacggca actactacta cggtatggac gtctggggcc aagggaccac ggtcaccgtc      360

tcctca                                                                 366


<210>  2
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of VH of anti-CD20 Fab

<400>  2

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Asp Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Ser Trp Asn Ser Gly Ser Ile Gly Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Lys Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asp Ile Gln Tyr Gly Asn Tyr Tyr Tyr Gly Met Asp Val Trp 
            100                 105                 110         


Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120         


<210>  3
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR1 in VH of anti-CD20 Fab

<400>  3

Gly Phe Thr Phe Asn Asp Tyr Ala 
1               5               


<210>  4
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR2 in VH of anti-CD20 Fab

<400>  4

Ile Ser Trp Asn Ser Gly Ser Ile 
1               5               


<210>  5
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR3 in VH of anti-CD20 Fab

<400>  5

Ala Lys Asp Ile Gln Tyr Gly Asn Tyr Tyr Tyr Gly Met Asp Val 
1               5                   10                  15  


<210>  6
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The nucleotide sequence coding for VL of anti-CD20 Fab

<400>  6
gaaattgtgt tgacacagtc tccagccacc ctgtctttgt ctccagggga aagagccacc       60

ctctcctgca gggccagtca gagtgttagc agctacttag cctggtacca acagaaacct      120

ggccaggctc ccaggctcct catctatgat gcatccaaca gggccactgg catcccagcc      180

aggttcagtg gcagtgggtc tgggacagac ttcactctca ccatcagcag cctagagcct      240

gaagattttg cagtttatta ctgtcagcag cgtagcaact ggccgatcac cttcggccaa      300

gggacacgac tggagattaa a                                                321


<210>  7
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of VL of anti-CD20 Fab

<400>  7

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Asn Trp Pro Ile 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105         


<210>  8
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR1 in VL of anti-CD20 Fab

<400>  8

Gln Ser Val Ser Ser Tyr 
1               5       


<210>  9
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR2 in VL of anti-CD20 Fab

<400>  9

Asp Ala Ser 
1           


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR3 in VL of anti-CD20 Fab

<400>  10

Gln Gln Arg Ser Asn Trp Pro Ile Thr 
1               5                   


<210>  11
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The nucleotide sequence coding for VH of anti-CD3 scFV (#1)

<400>  11
caggtgcagc tggtgcagag cggcggcggc gtggtgcagc ccggccgcag cctgcgcctg       60

agctgcaagg ccagcggcta caccttcacc cgctacacca tgcactgggt gcgccaggcc      120

cccggcaagg gcctggagtg gatcggctac atcaacccca gccgcggcta caccaactac      180

aaccagaagg tgaaggaccg cttcaccatc agcaccgaca agagcaagag caccgccttc      240

ctgcagatgg acagcctgcg ccccgaggac accgccgtgt actactgcgc ccgctactac      300

gacgaccact actcgctgga ctactggggc cagggcaccc ccgtgaccgt gtcctca         357


<210>  12
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of VH of anti-CD3 scFV (#1)

<400>  12

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Thr Asp Lys Ser Lys Ser Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser 
        115                 


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR1 in VH of anti-CD3 scFV

<400>  13

Gly Tyr Thr Phe Thr Arg Tyr Thr 
1               5               


<210>  14
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR2 in VH of anti-CD3 scFV

<400>  14

Ile Asn Pro Ser Arg Gly Tyr Thr 
1               5               


<210>  15
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR3 in VH of anti-CD3 scFV

<400>  15

Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr 
1               5                   10          


<210>  16
<211>  318
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The nucleotide sequence coding for VL of anti-CD3 scFV

<400>  16
gacatccaga tgacccagag ccccagcagc ctgagcgcca gcgtgggcga ccgcgtgacc       60

atcacctgca gcgccagcag cagcgtgagc tacatgaact ggtaccagca gacccccggc      120

aaggccccca agcgctggat ctacgacacc agcaagctgg ccagcggcgt gcccagccgc      180

ttcagcggca gcggcagcgg caccgactac accttcacca tcagcagcct gcagcccgag      240

gacatcgcca cctactactg ccagcagtgg agcagcaacc ccttcacctt cggccagggc      300

accaagctgc agatcacc                                                    318


<210>  17
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of VL of anti-CD3 scFV

<400>  17

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu 
65                  70                  75                  80  


Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr 
                85                  90                  95      


Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr 
            100                 105     


<210>  18
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR1 in VL of anti-CD3 scFV

<400>  18

Ser Ser Val Ser Tyr 
1               5   


<210>  19
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR2 in VL of anti-CD3 scFV

<400>  19

Asp Thr Ser 
1           


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of CDR3 in VL of anti-CD3 scFV

<400>  20

Gln Gln Trp Ser Ser Asn Pro Phe Thr 
1               5                   


<210>  21
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The nucleotide sequence coding for VH of anti-CD3 scFV (#2)

<400>  21
caggtgcagc tggtgcagag cggcggcggc gtggtgcagc ccggccgcag cctgcgcctg       60

agctgcaagg ccagcggcta caccttcacc cgctacacca tgcactgggt gcgccaggcc      120

cccggcaagg gcctggagtg gatcggctac atcaacccca gccgcggcta caccaactac      180

aaccagaagg tgaaggaccg cttcaccatc agccgcgaca atagcaagaa caccgccttc      240

ctgcagatgg acagcctgcg ccccgaggac accggcgtgt acttctgcgc ccgctactac      300

gacgaccact actcgctgga ctactggggc cagggcaccc ccgtgaccgt gtcctca         357


<210>  22
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of VH of anti-CD3 scFV (#2)

<400>  22

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Val 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Ala Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser 
        115                 


<210>  23
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  23
ggtcagcctg acctgcctgg tcaaaggct                                         29


<210>  24
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  24
tggttcttgg tcatctcctc ctgggatg                                          28


<210>  25
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  25
cttcttcctc tacagcaggc taaccg                                            26


<210>  26
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  26
gagccgtcgg agtccagcac gggaggc                                           27


<210>  27
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  27
aaccaggtca gcctgtggtg cctggtcaaa ggcttctacc                             40


<210>  28
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  28
cttggtcatc tcctcctggg atgggggcag ggtgtaca                               38


