                         SEQUENCE LISTING

<110>  Ward, Brian J
       Winter, Kaitlin G. F.
 
<120>  VACCINE AND METHOD OF VACCINATION AGAINST CLOSTRIDIUM DIFFICILE 
       USING A LIVE BACTERIAL VECTOR

<130>  Aviex-210.1

<150>  62734103
<151>  2018-09-20

<150>  62803167
<151>  2019-02-08

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SopE2 promoter and secretory signal forward primer

<400>  1
ccgctcgagt aaaaatgttc ctcgataaa                                         29


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SopE2 promoter and secretory signal reverse primer

<400>  2
catggtagtt ctccttttag                                                   20


<210>  3
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SptP promoter and secretory signal forward primer

<400>  3
cgcctcgagt ttacgctgac tcattgg                                           27


<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SptP promoter and secretory signal reverse primer

<400>  4
catttttctc tcctcatact tta                                               23


<210>  5
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SseJ promoter and secretory signal forward primer

<400>  5
cgcctcgaga cataaaacac tagcact                                           27


<210>  6
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SseJ promoter and secretory signal reverse primer

<400>  6
cgcctcgaga cataaaacac tagcact                                           27


<210>  7
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SspH1 promoter and secretory signal forward primer

<400>  7
cgcctcgagc gctatatcac caaaac                                            26


<210>  8
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SspH1 promoter and secretory signal reverse primer

<400>  8
ctctgcggcc gcggtaagac ctgacgctc                                         29


<210>  9
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SspH2 promoter and secretory signal forward primer

<400>  9
cgcctcgagg tttgtgcgtc gtat                                              24


<210>  10
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SspH2 promoter and secretory signal reverse primer

<400>  10
ctctgcggcc gcattcaggc aggcacgca                                         29


<210>  11
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SteA promoter and secretory signal forward primer

<400>  11
cgcctcgagg tttcgccgca tgttg                                             25


<210>  12
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SteA promoter and secretory signal reverse primer

<400>  12
ctctgcggcc gcataattgt ccaaatagt                                         29


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SteB promoter and secretory signal forward primer

<400>  13
cgcctcgagc gctccagcgc ttcga                                             25


<210>  14
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SteB promoter and secretory signal reverse primer

<400>  14
ctctgcggcc gctctgacat taccattt                                          28


<210>  15
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lac promoter

<400>  15
cgcctcgagc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt       60

ggaattgtga gcggataa                                                     78


<210>  16
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lac promoter

<400>  16
gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gactaacata       60

acactatcca c                                                            71


<210>  17
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DH5D E. coli nirB promoter forward primer

<400>  17
cgcctcgagt tgtggttacc ggcccgat                                          28


<210>  18
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DH5D E. coli nirB promoter reverse primer

<400>  18
cgcgcggccg ccggatcttt actcgcatta c                                      31


<210>  19
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 pagC promoter forward primer

<400>  19
cgcctcgagg ttaaccactc ttaataa                                           27


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 pagC promoter reverse primer

<400>  20
aacaactcct taatactact                                                   20


<210>  21
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SopE2 Secretion Signal forward primer

<400>  21
ggcggtaata gaaaagaaat cgaggcaaaa atgactaaca taacactatc cac              53


<210>  22
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SopE2 Secretion Signal reverse primer

<400>  22
aagtcgcggc cgccggatct ttactcgc                                          28


<210>  23
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SspH1 Secretion Signal forward primer

<400>  23
ggcggtaata gaaaagaaat cgaggcaaaa atgtttaata tccgcaatac acaacctt         58


<210>  24
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YS1646 SspH1 Secretion Signal reverse primer

<400>  24
ctctgcggcc gcggtaagac ctgacgctc                                         29


<210>  25
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VPI 10463 rTcdA forward primer

<400>  25
cgcgcggccg cgacttatta ctatgat                                           27


<210>  26
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VPI 10463 rTcdA reverse primer

<400>  26
tagtcggcgc gcccgccata tatcccagg                                         29


<210>  27
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VPI 10463 rTcdB forward primer

<400>  27
ccggcggccg cagagaaatt ttatattaat                                        30


<210>  28
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VPI 10463 rTcdB reverse primer

<400>  28
agtcggcgcg ccgttcacta atcactaatt g                                      31


<210>  29
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEGFP_C1 EGFP forward primer

<400>  29
cgcgcggccg cggtgagcaa gggcgag                                           27


<210>  30
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEGFP_C1 EGFP reverse primer

<400>  30
agtcggcgcg ccttacttgt acagctcgtc                                        30


