                         SEQUENCE LISTING

<110>  Universit degli Studi di Pavia
 
<120>  An antibody drug conjugate based on asparaginase

<130>  @E0115967

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  611
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein

<400>  1

Met Gly His His His His His His Glu Val Lys Leu Gln Glu Ser Gly 
1               5                   10                  15      


Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val 
            20                  25                  30          


Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro 
        35                  40                  45              


Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr 
    50                  55                  60                  


Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp 
65                  70                  75                  80  


Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp 
                85                  90                  95      


Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser 
            100                 105                 110         


Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val 
    130                 135                 140                 


Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
145                 150                 155                 160 


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
                165                 170                 175     


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
            180                 185                 190         


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
        195                 200                 205             


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
    210                 215                 220                 


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
225                 230                 235                 240 


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser Gly Gly Gly 
                245                 250                 255     


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            260                 265                 270         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Leu Pro Asn 
        275                 280                 285             


Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly Gly Asp Ser 
    290                 295                 300                 


Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val Glu Asn Leu 
305                 310                 315                 320 


Val Asn Ala Val Pro Gln Leu Lys Asp Ile Ala Asn Val Lys Gly Glu 
                325                 330                 335     


Gln Val Val Asn Ile Gly Ser Gln Asp Met Asn Asp Asn Val Trp Leu 
            340                 345                 350         


Thr Leu Ala Lys Lys Ile Asn Thr Asp Cys Asp Lys Thr Asp Gly Phe 
        355                 360                 365             


Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala Tyr Phe Leu 
    370                 375                 380                 


Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val Gly Ala Met 
385                 390                 395                 400 


Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn Leu Tyr Asn 
                405                 410                 415     


Ala Val Val Thr Ala Ala Asp Lys Ala Ser Ala Asn Arg Gly Val Leu 
            420                 425                 430         


Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val Thr Lys Thr 
        435                 440                 445             


Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr Gly Pro Leu 
    450                 455                 460                 


Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr Pro Ala Arg 
465                 470                 475                 480 


Lys His Thr Ser Asp Thr Pro Phe Asp Val Ser Lys Leu Asn Glu Leu 
                485                 490                 495     


Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser Asp Leu Pro 
            500                 505                 510         


Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val Ser Ala Gly 
        515                 520                 525             


Val Gly Asn Gly Asn Leu Tyr Lys Ser Val Phe Asp Thr Leu Ala Thr 
    530                 535                 540                 


Ala Ala Lys Thr Gly Thr Ala Val Val Arg Ser Ser Arg Val Pro Thr 
545                 550                 555                 560 


Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys Tyr Gly Phe 
                565                 570                 575     


Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val Leu Leu Gln 
            580                 585                 590         


Leu Ala Leu Thr Gln Thr Lys Asp Pro Gln Gln Ile Gln Gln Ile Phe 
        595                 600                 605             


Asn Gln Tyr 
    610     


<210>  2
<211>  1842
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence encoding Fusion protein

<400>  2
atgggtcatc atcatcatca tcatgaggtg aaactgcagg agtcaggacc tggcctggtg       60

gcgccctcac agagcctgtc cgtcacatgc actgtctcag gggtctcatt acccgactat      120

ggtgtaagct ggattcgcca gcctccacga aagggtctgg agtggctggg agtaatatgg      180

ggtagtgaaa ccacatacta taattcagct ctcaaatcca gactgaccat catcaaggac      240

aactccaaga gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccatt      300

tactactgtg ccaaacatta ttactacggt ggtagctatg ctatggacta ctggggtcaa      360

ggaacctcag tcaccgtctc gagtggtgga ggcggttcag gcggaggtgg ctctggcggt      420

ggcggtagtg tcgacatcca gatgacacag actacatcct ccctgtctgc ctctctggga      480

gacagagtca ccatcagttg cagggcaagt caggacatta gtaaatattt aaattggtat      540

cagcagaaac cagatggaac tgttaaactc ctgatctacc atacatcaag attacactca      600

ggagtcccat caaggttcag tggcagtggg tctggaacag attattctct caccattagc      660

aacctggagc aagaagatat tgccacttac ttttgccaac agggtaatac gcttccgtac      720

acgttcggag gggggactaa gcttgaaata acaggatccg gcggaggtgg ctctggcggt      780

ggtggctctg gtggaggcgg ttcaggcgga ggtggctctg gcggtggtgg ctctggtgga      840

ggcggttcag agctcttacc caatatcacc attttagcaa ccggcgggac cattgccggt      900

ggtggtgact ccgcaaccaa atctaactac acagtgggta aagttggcgt agaaaatctg      960

gttaatgcgg tgccgcaact aaaagacatt gcgaacgtta aaggcgagca ggtagtgaat     1020

atcggctccc aggacatgaa cgataatgtc tggctgacac tggcgaaaaa aattaacacc     1080

gactgcgata agaccgacgg cttcgtcatt acccacggta ccgacacgat ggaagaaact     1140

gcttacttcc tcgacctgac ggtgaaatgc gacaaaccgg tggtgatggt cggcgcaatg     1200

cgtccgtcca cgtctatgag cgcagacggt ccattcaacc tgtataacgc ggtagtgacc     1260

gcagctgata aagcctccgc caaccgtggc gtgctggtag tgatgaatga caccgtgctt     1320

gatggccgtg acgtcaccaa aaccaacacc accgacgtag cgaccttcaa gtctgttaac     1380

tacggtcctc tgggttacat tcacaacggt aagattgact accagcgtac cccggcacgt     1440

aagcatacca gcgacacgcc attcgatgtc tctaagctga atgaactgcc gaaagtcggc     1500

attgtttata actacgctaa cgcatccgat cttccggcta aagcactggt agatgcgggc     1560

tatgatggca tcgttagcgc tggtgtgggt aacggcaacc tgtataaatc tgtgttcgac     1620

acgctggcga ccgccgcgaa aaccggtact gcagtcgtgc gttcttcccg cgtaccgacg     1680

ggcgctacca ctcaggatgc cgaagtggat gatgcgaaat acggcttcgt cgcctctggc     1740

acgctgaacc cgcaaaaagc gcgcgttctg ctgcaactgg ctctgacgca aaccaaagat     1800

ccgcagcaga tccagcagat cttcaatcag tactaataat aa                        1842


<210>  3
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer scFvfusion_s

<400>  3
ccatgggtca tcatcatcat catcatgagg tgaaactgca ggagtcagg                   49


<210>  4
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer scFvfusion_as

<400>  4
ggatcctgtt atttcaagct tagtcccccc tccg                                   34


<210>  5
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcAIIfusion_s

<400>  5
gagctcttac ccaatatcac cattttagc                                         29


<210>  6
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcAIIfusion_as

<400>  6
cctaggttat tattagtact gattgaagat ctgc                                   34


