                         б

<110>  Ŵҩ()޹˾
 
<120>  Ͱ׽2;
<130>  PF190556PCT

<160>  13    

<170>  PatentIn version 3.3

<210>  1
<211>  133
<212>  PRT
<213>  artificial sequence

<220>
<223>  IL-2WT with mutations(C125S)

<400>  1

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  2
<211>  133
<212>  PRT
<213>  artificial sequence

<220>
<223>  IL-2 3X with mutations(C125S,R38D, K43E, E61R)

<400>  2

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Asp Met Leu Thr Phe Glu Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Arg Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  3
<211>  133
<212>  PRT
<213>  artificial sequence

<220>
<223>  IL-2H9 with mutations(C125S,L80F,R81D,L85V,I86V, I92F)

<400>  3

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Phe 
65                  70                  75                  80  


Asp Pro Arg Asp Val Val Ser Asn Ile Asn Val Phe Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  4
<211>  4827
<212>  DNA
<213>  artificial sequence

<220>
<223>  yeast display plasmid pYDC011 

<400>  4
acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt     60

cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga    120

acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac    180

ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga    240

ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat    300

taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc    360

ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac    420

ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac    480

gactcactat agggaatatt aagctaattc cctacttcat acattttcaa ttaagatgca    540

gttacttcgc tgtttttcaa tattttctgt tattgcttca gttttagcag gatcctgaca    600

tagtagggat tataaggagg cggtggatcc gattacaagg atgacgatga caagggcgga    660

ggaggctccc aggaactgac aactatatgc gagcaaatcc cctcaccaac tttagaatcg    720

acgccgtact ctttgtcaac gactactatt ttggccaacg ggaaggcaat gcaaggagtt    780

tttgaatatt acaaatcagt aacgtttgtc agtaattgcg gttctcaccc ctcaacgact    840

agcaaaggca gccccataaa cacacagtat gttttttaat gagtttaaac ccgctgatct    900

gataacaaca gtgtagatgt aacaaaatcg actttgttcc cactgtactt ttagctcgta    960

caaaatacaa tatacttttc atttctccgt aaacaacatg ttttcccatg taatatcctt   1020

ttctattttt cgttccgtta ccaactttac acatacttta tatagctatt cacttctata   1080

cactaaaaaa ctaagacaat tttaattttg ctgcctgcca tatttcaatt tgttataaat   1140

tcctataatt tatcctatta gtagctaaaa aaagatgaat gtgaatcgaa tcctaagaga   1200

attgggcaag tgcacaaaca atacttaaat aaatactact cagtaataac ctatttctta   1260

gcatttttga cgaaatttgc tattttgtta gagtctttta caccatttgt ctccacacct   1320

ccgcttacat caacaccaat aacgccattt aatctaagcg catcaccaac attttctggc   1380

gtcagtccac cagctaacat aaaatgtaag ctctcggggc tctcttgcct tccaacccag   1440

tcagaaatcg agttccaatc caaaagttca cctgtcccac ctgcttctga atcaaacaag   1500

ggaataaacg aatgaggttt ctgtgaagct gcactgagta gtatgttgca gtcttttgga   1560

aatacgagtc ttttaataac tggcaaaccg aggaactctt ggtattcttg ccacgactca   1620

tctccgtgca gttggacgat atcaatgccg taatcattga ccagagccaa aacatcctcc   1680

ttaggttgat tacgaaacac gccaaccaag tatttcggag tgcctgaact atttttatat   1740

gcttttacaa gacttgaaat tttccttgca ataaccgggt caattgttct ctttctattg   1800

ggcacacata taatacccag caagtcagca tcggaatcta gagcacattc tgcggcctct   1860

gtgctctgca agccgcaaac tttcaccaat ggaccagaac tacctgtgaa attaataaca   1920

gacatactcc aagctgcctt tgtgtgctta atcacgtata ctcacgtgct caatagtcac   1980

caatgccctc cctcttggcc ctctcctttt cttttttcga ccgaatttct tgaagacgaa   2040

agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagg   2100

acggatcgct tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg aataatttgg   2160

gaatttactc tgtgtttatt tatttttatg ttttgtattt ggattttaga aagtaaataa   2220

agaaggtaga agagttacgg aatgaagaaa aaaaaataaa caaaggttta aaaaatttca   2280

acaaaaagcg tactttacat atatatttat tagacaagaa aagcagatta aatagatata   2340

cattcgatta acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca   2400

cagacaagat gaaacaattc ggcattaata cctgagagca ggaagagcaa gataaaaggt   2460

agtatttgtt ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa aactattttt   2520

tctttaattt ctttttttac tttctatttt taatttatat atttatatta aaaaatttaa   2580

attataatta tttttatagc acgtgatgaa aaggacccag gtggcacttt tcggggaaat   2640

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg   2700

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa   2760

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac   2820

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac   2880

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt   2940

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc   3000

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca   3060

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc   3120

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag   3180

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa   3240

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg   3300

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa   3360

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg   3420

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt   3480

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacgggcagt   3540

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag   3600

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat   3660

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct   3720

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct   3780

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca   3840

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc   3900

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc   3960

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct   4020

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag   4080

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc   4140

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg   4200

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag   4260

cttccagggg ggaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt   4320

gagcgtcgat ttttgtgatg ctcgtcaggg gggccgagcc tatggaaaaa cgccagcaac   4380

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg   4440

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc   4500

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata   4560

cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt   4620

cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttacct cactcattag   4680

gcaccccagg ctttacactt tatgcttccg gctcctatgt tgtgtggaat tgtgagcgga   4740

taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcgg aattaaccct   4800

cactaaaggg aacaaaagct ggctagt                                       4827


<210>  5
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer AMP0191

<400>  5
cccggatcgg actactagca gc                                              22


<210>  6
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer AMP0199

<400>  6
ctccttgcat tgccttcccg ttg                                             23


<210>  7
<211>  42
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer AMP0210

<400>  7
gttattgctt cagttttagc agctcccacc agcagcagca cc                        42


<210>  8
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer AMP0211

<400>  8
catcgtcatc cttgtaatcg gatccaccgc ctccggtcag tgtgctgatg atgc           54


<210>  9
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer AMP0224

<400>  9
ctgggtcttc ttggtgctgc                                                 20


<210>  10
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer AMP0225

<400>  10
gaagttcttg ctctgggcta aattg                                           25


<210>  11
<211>  217
<212>  PRT
<213>  artificial sequence

<220>
<223>  IL-2R with C-terminal avi tag and His6 tag

<400>  11

Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe Lys 
1               5                   10                  15      


Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys Arg 
            20                  25                  30          


Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr Gly 
        35                  40                  45              


Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser Ser 
    50                  55                  60                  


Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu Gln 
65                  70                  75                  80  


Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val Asp 
                85                  90                  95      


Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu Asn 
            100                 105                 110         


Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val Tyr 
        115                 120                 125             


Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala Glu 
    130                 135                 140                 


Ser Val Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro Gln 
145                 150                 155                 160 


Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu Glu 
                165                 170                 175     


Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser Cys 
            180                 185                 190         


Leu Val Thr Thr Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu 
        195                 200                 205             


Trp His Glu His His His His His His 
    210                 215         


<210>  12
<211>  235
<212>  PRT
<213>  artificial sequence

<220>
<223>  IL-2R with C-terminal avi tag and His6 tag

<400>  12

Ala Val Asn Gly Thr Ser Gln Phe Thr Cys Phe Tyr Asn Ser Arg Ala 
1               5                   10                  15      


Asn Ile Ser Cys Val Trp Ser Gln Asp Gly Ala Leu Gln Asp Thr Ser 
            20                  25                  30          


Cys Gln Val His Ala Trp Pro Asp Arg Arg Arg Trp Asn Gln Thr Cys 
        35                  40                  45              


Glu Leu Leu Pro Val Ser Gln Ala Ser Trp Ala Cys Asn Leu Ile Leu 
    50                  55                  60                  


Gly Ala Pro Asp Ser Gln Lys Leu Thr Thr Val Asp Ile Val Thr Leu 
65                  70                  75                  80  


Arg Val Leu Cys Arg Glu Gly Val Arg Trp Arg Val Met Ala Ile Gln 
                85                  90                  95      


Asp Phe Lys Pro Phe Glu Asn Leu Arg Leu Met Ala Pro Ile Ser Leu 
            100                 105                 110         


Gln Val Val His Val Glu Thr His Arg Cys Asn Ile Ser Trp Glu Ile 
        115                 120                 125             


Ser Gln Ala Ser His Tyr Phe Glu Arg His Leu Glu Phe Glu Ala Arg 
    130                 135                 140                 


Thr Leu Ser Pro Gly His Thr Trp Glu Glu Ala Pro Leu Leu Thr Leu 
145                 150                 155                 160 


Lys Gln Lys Gln Glu Trp Ile Cys Leu Glu Thr Leu Thr Pro Asp Thr 
                165                 170                 175     


Gln Tyr Glu Phe Gln Val Arg Val Lys Pro Leu Gln Gly Glu Phe Thr 
            180                 185                 190         


Thr Trp Ser Pro Trp Ser Gln Pro Leu Ala Phe Arg Thr Lys Pro Ala 
        195                 200                 205             


Ala Leu Gly Lys Asp Thr Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys 
    210                 215                 220                 


Ile Glu Trp His Glu His His His His His His 
225                 230                 235 


<210>  13
<211>  6147
<212>  DNA
<213>  artificial sequence 

<220>
<223>  vector pYDO_017

<400>  13
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg     60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg    120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc    180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt    240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata    300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc    360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc    420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt    480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt    540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca    600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg    660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc    720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg    780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca    840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggatgga    900

gaccgacacc ctcttactgt gggtgctgct gctgtgggtt cccggttcca ctggatcctg    960

acatagtagg gattataagg aggcggtgga tccggcggcg gaggctccga caaaacccat   1020

acatgtcctc cttgccccgc ccctgaggct gctggaggcc ccagcgtgtt cctgtttccc   1080

cccaagccca aagataccct catgatctcc aggacccccg aagtgacctg cgtcgtggtc   1140

gacgtgagcc acgaggaccc tgaagtcaag ttcaactggt acgtcgatgg cgtggaggtg   1200

cacaacgcta agaccaaacc ccgggaagag cagtacaatt ccacctacag ggtggtgtcc   1260

gtcctgacag tgctgcacca agactggctg aatggaaagg agtacaagtg caaagtgagc   1320

aataaggccc tccctgctcc cattgagaag accatttcca aggccaaagg ccagcctcgg   1380

gaaccccagg tgtacacact gcccccttcc agggaggaga tgaccaagaa ccaggtgagc   1440

ctcacctgcc tggtgaaggg cttctaccct agcgacattg ctgtggagtg ggagagcaac   1500

ggccagcccg aaaacaacta taagacaacc cctcccgtgc tggacagcga cggctccttc   1560

tttctgtact ccaagctcac cgtggacaag tccaggtggc aacagggaaa cgtgttctcc   1620

tgctccgtga tgcacgaggc cctccacaac cactacaccc agaagagcct gagcctgtcc   1680

cctggcaagt gatgaagcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag   1740

cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct   1800

tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc   1860

attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg   1920

aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg   1980

cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa   2040

gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc   2100

ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag   2160

ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca   2220

aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc   2280

gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa   2340

cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct   2400

attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt   2460

gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat   2520

gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag   2580

tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat   2640

cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt   2700

tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg   2760

cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg   2820

atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc   2880

aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat   2940

cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt   3000

caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg   3060

gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag   3120

ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc   3180

tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc   3240

tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga   3300

agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga   3360

actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg   3420

cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg   3480

tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc   3540

tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc   3600

cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg   3660

gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc   3720

gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc   3780

ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct   3840

tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca   3900

ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg   3960

tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt   4020

tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt   4080

gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg   4140

ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg   4200

cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg   4260

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat   4320

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc   4380

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc   4440

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga   4500

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt   4560

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg   4620

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc   4680

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg   4740

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc   4800

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg   4860

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc   4920

gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa   4980

gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa   5040

gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa   5100

tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc   5160

ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga   5220

ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca   5280

atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc   5340

ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat   5400

tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc   5460

attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt   5520

tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc   5580

ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg   5640

gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt   5700

gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg   5760

gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga   5820

aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg   5880

taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg   5940

tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt   6000

tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc   6060

atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca   6120

tttccccgaa aagtgccacc tgacgtc                                       6147


