                         SEQUENCE LISTING

<110>  WuXi Biologics (Shanghai) CO., LTD.
       Wuxi Biologics Ireland Limited
 
<120>  NOVEL BISPECIFIC ANTI-CTLA-4/PD-1 POLYPEPTIDE COMPLEXES

<130>  IEC180184PCT WBP3248

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  116
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  1

Glu Val Ala Val Phe Glu Pro Ser Glu Ala Glu Ile Ser His Thr Gln 
1               5                   10                  15      


Lys Ala Thr Leu Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val 
            20                  25                  30          


Glu Leu Ser Trp Trp Val Asn Gly Lys Glu Val His Ser Gly Val Cys 
        35                  40                  45              


Thr Asp Pro Gln Pro Leu Lys Glu Gln Pro Ala Leu Gln Asp Ser Arg 
    50                  55                  60                  


Tyr Ala Leu Ser Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn 
65                  70                  75                  80  


Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu 
                85                  90                  95      


Asn Asp Glu Trp Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val 
            100                 105                 110         


Ser Ala Glu Ala 
        115     


<210>  2
<211>  87
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  2

Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser Ser Asp Lys Ser Val Cys 
1               5                   10                  15      


Leu Phe Thr Asp Phe Asp Ser Gln Thr Gln Val Ser Gln Ser Lys Asp 
            20                  25                  30          


Ser Asp Val Tyr Ile Thr Asp Lys Cys Val Leu Asp Met Arg Ser Met 
        35                  40                  45              


Asp Phe Lys Ser Asn Ser Ala Val Ala Trp Ser Gln Lys Ser Asp Phe 
    50                  55                  60                  


Ala Cys Ala Asn Ala Phe Gln Asn Ser Ile Ile Pro Glu Asp Thr Phe 
65                  70                  75                  80  


Phe Pro Ser Pro Glu Ser Ser 
                85          


<210>  3
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  3

Leu Glu Asp Leu Lys Asn Val Phe Pro Pro 
1               5                   10  


<210>  4
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  4

Pro Asp Ile Gln Asn Pro Asp Pro 
1               5               


<210>  5
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR1

<400>  5

Gly Phe Thr Phe Ser Ser Tyr Thr Met His 
1               5                   10  


<210>  6
<211>  17
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR2

<400>  6

Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  7
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR3

<400>  7

Thr Gly Trp Leu Gly Pro Phe Asp Tyr 
1               5                   


<210>  8
<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR1

<400>  8

Arg Ala Ser Gln Ser Val Gly Ser Ser Tyr Leu Ala 
1               5                   10          


<210>  9
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR2

<400>  9

Gly Ala Phe Ser Arg Ala Thr 
1               5           


<210>  10
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR3

<400>  10

Gln Gln Tyr Gly Ser Ser Pro Trp Thr 
1               5                   


<210>  11
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR1

<400>  11

Gly Tyr Thr Phe Thr Asn Tyr Phe Met Asn 
1               5                   10  


<210>  12
<211>  17
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR2

<400>  12

Arg Val Asp Pro Glu Gln Gly Arg Ala Asp Tyr Ala Glu Lys Phe Lys 
1               5                   10                  15      


Lys 
    


<210>  13
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR3

<400>  13

Arg Ala Met Asp Asn Tyr Gly Phe Ala Tyr 
1               5                   10  


<210>  14
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR1

<400>  14

Ser Ala Asn Ser Ala Leu Ser Tyr Met Tyr 
1               5                   10  


<210>  15
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR2

<400>  15

Gly Thr Ser Asn Leu Ala Ser 
1               5           


<210>  16
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR3

<400>  16

His His Trp Ser Asn Thr Gln Trp Thr 
1               5                   


<210>  17
<211>  116
<212>  PRT
<213>  artificial sequence

<220>
<223>  VH

<400>  17

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val 
        115     


<210>  18
<211>  108
<212>  PRT
<213>  artificial sequence

<220>
<223>  VL

<400>  18

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro 
                85                  90                  95      


Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105             


<210>  19
<211>  117
<212>  PRT
<213>  artificial sequence

<220>
<223>  VH

<400>  19

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Phe Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Val Asp Pro Glu Gln Gly Arg Ala Asp Tyr Ala Glu Lys Phe 
    50                  55                  60                  


Lys Lys Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Ala Met Asp Asn Tyr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val 
        115         


<210>  20
<211>  106
<212>  PRT
<213>  artificial sequence

<220>
<223>  VL

<400>  20

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Ser Ala Asn Ser Ala Leu Ser Tyr Met 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Trp Val His 
        35                  40                  45              


Gly Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys His His Trp Ser Asn Thr Gln Trp Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105     


<210>  21
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR1

<400>  21

Gly Phe Thr Phe Ser Ser His Ala Met Ser 
1               5                   10  


<210>  22
<211>  17
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR2

<400>  22

Thr Ile Thr Gly Gly Gly Gly Ser Ile Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  23
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR3

<400>  23

Asn Arg Ala Gly Glu Gly Tyr Phe Asp Tyr 
1               5                   10  


<210>  24
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR1

<400>  24

Gly Gly Asp Asn Ile Gly Asn Lys Asp Val His 
1               5                   10      


<210>  25
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR2

<400>  25

Arg Asp Ser Asn Arg Pro Ser 
1               5           


<210>  26
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR3

<400>  26

Gln Val Trp Asp Ser Ile Trp Val 
1               5               


<210>  27
<211>  117
<212>  PRT
<213>  artificial sequence

<220>
<223>  VH

<400>  27

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser His 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Thr Gly Gly Gly Gly Ser Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asn Arg Ala Gly Glu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val 
        115         


<210>  28
<211>  105
<212>  PRT
<213>  artificial sequence

<220>
<223>  VL

<400>  28

Ser Tyr Glu Leu Thr Gln Pro Leu Ser Val Ser Val Ala Leu Gly Gln 
1               5                   10                  15      


Thr Ala Arg Ile Thr Cys Gly Gly Asp Asn Ile Gly Asn Lys Asp Val 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr 
        35                  40                  45              


Arg Asp Ser Asn Arg Pro Ser Gly Ile Pro Glu Gly Phe Ser Gly Ser 
    50                  55                  60                  


Asn Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Arg Ala Gln Ala Gly 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Val Trp Asp Ser Ile Trp Val Phe 
                85                  90                  95      


Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105 


<210>  29
<211>  211
<212>  PRT
<213>  artificial sequence

<220>
<223>  U6-LC-G25R

<400>  29

Ser Tyr Glu Leu Thr Gln Pro Leu Ser Val Ser Val Ala Leu Gly Gln 
1               5                   10                  15      


Thr Ala Arg Ile Thr Cys Gly Gly Asp Asn Ile Gly Asn Lys Asp Val 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr 
        35                  40                  45              


Arg Asp Ser Asn Arg Pro Ser Gly Ile Pro Glu Gly Phe Ser Gly Ser 
    50                  55                  60                  


Asn Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Arg Ala Gln Ala Gly 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Val Trp Asp Ser Ile Trp Val Phe 
                85                  90                  95      


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro Lys Ala Ala Pro 
            100                 105                 110         


Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln Ala Asn Lys 
        115                 120                 125             


Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly Ala Val Thr 
    130                 135                 140                 


Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala Gly Val Glu Thr 
145                 150                 155                 160 


Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala Ser Ser Tyr 
                165                 170                 175     


Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Lys Ser Tyr Ser Cys 
            180                 185                 190         


Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr Val Ala Pro Thr 
        195                 200                 205             


Glu Cys Ser 
    210     


<210>  30
<211>  203
<212>  PRT
<213>  artificial sequence

<220>
<223>  T1-LC-G25R

<400>  30

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro 
                85                  90                  95      


Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Pro Asp Ile Gln 
            100                 105                 110         


Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser Ser Asp 
        115                 120                 125             


Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Gln Val Ser 
    130                 135                 140                 


Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys Val Leu Asp 
145                 150                 155                 160 


Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp Ser Gln 
                165                 170                 175     


Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Gln Asn Ser Ile Ile Pro 
            180                 185                 190         


Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser 
        195                 200             


<210>  31
<211>  697
<212>  PRT
<213>  artificial sequence

<220>
<223>  U6-T1-HC-G25R

<400>  31

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser His 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Thr Gly Gly Gly Gly Ser Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asn Arg Ala Gly Glu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             


Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         


Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro 
        195                 200                 205             


Ser Asn Thr Lys Val Asp Lys Arg Val Gly Gly Gly Gly Ser Gly Gly 
    210                 215                 220                 


Gly Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln 
225                 230                 235                 240 


Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
                245                 250                 255     


Ser Ser Tyr Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
            260                 265                 270         


Glu Trp Val Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala 
        275                 280                 285             


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
    290                 295                 300                 


Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile 
305                 310                 315                 320 


Tyr Tyr Cys Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly 
                325                 330                 335     


Gln Gly Thr Leu Val Thr Val Leu Glu Asp Leu Lys Asn Val Phe Pro 
            340                 345                 350         


Pro Glu Val Ala Val Phe Glu Pro Ser Glu Ala Glu Ile Ser His Thr 
        355                 360                 365             


Gln Lys Ala Thr Leu Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His 
    370                 375                 380                 


Val Glu Leu Ser Trp Trp Val Asn Gly Lys Glu Val His Ser Gly Val 
385                 390                 395                 400 


Cys Thr Asp Pro Gln Pro Leu Lys Glu Gln Pro Ala Leu Gln Asp Ser 
                405                 410                 415     


Arg Tyr Ala Leu Ser Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln 
            420                 425                 430         


Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser 
        435                 440                 445             


Glu Asn Asp Glu Trp Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile 
    450                 455                 460                 


Val Ser Ala Glu Ala Trp Gly Arg Tyr Gly Pro Pro Cys Pro Pro Cys 
465                 470                 475                 480 


Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
                485                 490                 495     


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
            500                 505                 510         


Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp 
        515                 520                 525             


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
    530                 535                 540                 


Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
545                 550                 555                 560 


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
                565                 570                 575     


Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
            580                 585                 590         


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu 
        595                 600                 605             


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
    610                 615                 620                 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
625                 630                 635                 640 


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
                645                 650                 655     


Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn 
            660                 665                 670         


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
        675                 680                 685             


Gln Lys Ser Leu Ser Leu Ser Leu Gly 
    690                 695         


<210>  32
<211>  200
<212>  PRT
<213>  artificial sequence

<220>
<223>  U6-LC-G25

<400>  32

Ser Tyr Glu Leu Thr Gln Pro Leu Ser Val Ser Val Ala Leu Gly Gln 
1               5                   10                  15      


Thr Ala Arg Ile Thr Cys Gly Gly Asp Asn Ile Gly Asn Lys Asp Val 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr 
        35                  40                  45              


Arg Asp Ser Asn Arg Pro Ser Gly Ile Pro Glu Gly Phe Ser Gly Ser 
    50                  55                  60                  


Asn Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Arg Ala Gln Ala Gly 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Val Trp Asp Ser Ile Trp Val Phe 
                85                  90                  95      


Gly Gly Gly Thr Lys Leu Thr Val Leu Pro Asp Ile Gln Asn Pro Asp 
            100                 105                 110         


Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser Ser Asp Lys Ser Val 
        115                 120                 125             


Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Gln Val Ser Gln Ser Lys 
    130                 135                 140                 


Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys Val Leu Asp Met Arg Ser 
145                 150                 155                 160 


Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp Ser Gln Lys Ser Asp 
                165                 170                 175     


Phe Ala Cys Ala Asn Ala Phe Gln Asn Ser Ile Ile Pro Glu Asp Thr 
            180                 185                 190         


Phe Phe Pro Ser Pro Glu Ser Ser 
        195                 200 


<210>  33
<211>  213
<212>  PRT
<213>  artificial sequence

<220>
<223>  T5-LC-G25

<400>  33

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Ser Ala Asn Ser Ala Leu Ser Tyr Met 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Trp Val His 
        35                  40                  45              


Gly Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys His His Trp Ser Asn Thr Gln Trp Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro 
            100                 105                 110         


Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr 
        115                 120                 125             


Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys 
    130                 135                 140                 


Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu 
145                 150                 155                 160 


Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser 
                165                 170                 175     


Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala 
            180                 185                 190         


Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe 
        195                 200                 205             


Asn Arg Gly Glu Cys 
    210             


<210>  34
<211>  701
<212>  PRT
<213>  artificial sequence

<220>
<223>  U6-T5-HC-G25

<400>  34

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser His 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Thr Gly Gly Gly Gly Ser Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asn Arg Ala Gly Glu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu 
        115                 120                 125             


Val Ala Val Phe Glu Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys 
    130                 135                 140                 


Ala Thr Leu Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu 
145                 150                 155                 160 


Leu Ser Trp Trp Val Asn Gly Lys Glu Val His Ser Gly Val Cys Thr 
                165                 170                 175     


Asp Pro Gln Pro Leu Lys Glu Gln Pro Ala Leu Gln Asp Ser Arg Tyr 
            180                 185                 190         


Ala Leu Ser Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro 
        195                 200                 205             


Arg Asn His Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn 
    210                 215                 220                 


Asp Glu Trp Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser 
225                 230                 235                 240 


Ala Glu Ala Trp Gly Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
                245                 250                 255     


Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
            260                 265                 270         


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
        275                 280                 285             


Phe Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
    290                 295                 300                 


Gly Arg Val Asp Pro Glu Gln Gly Arg Ala Asp Tyr Ala Glu Lys Phe 
305                 310                 315                 320 


Lys Lys Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
                325                 330                 335     


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
            340                 345                 350         


Ala Arg Arg Ala Met Asp Asn Tyr Gly Phe Ala Tyr Trp Gly Gln Gly 
        355                 360                 365             


Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
    370                 375                 380                 


Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu 
385                 390                 395                 400 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
                405                 410                 415     


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
            420                 425                 430         


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
        435                 440                 445             


Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro 
    450                 455                 460                 


Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro 
465                 470                 475                 480 


Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe 
                485                 490                 495     


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            500                 505                 510         


Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val 
        515                 520                 525             


Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    530                 535                 540                 


Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 
545                 550                 555                 560 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                565                 570                 575     


Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser 
            580                 585                 590         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
        595                 600                 605             


Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
    610                 615                 620                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
625                 630                 635                 640 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                645                 650                 655     


Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp 
            660                 665                 670         


Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
        675                 680                 685             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
    690                 695                 700     


<210>  35
<211>  93
<212>  PRT
<213>  homo sapiens

<220>
<223>  TRAC_Human

<400>  35

Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
1               5                   10                  15      


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
            20                  25                  30          


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr Val 
        35                  40                  45              


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
    50                  55                  60                  


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
65                  70                  75                  80  


Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser 
                85                  90              


<210>  36
<211>  95
<212>  PRT
<213>  artificial sequence

<220>
<223>  4L4T_Alpha_Crystal

<400>  36

Pro Asp Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser 
1               5                   10                  15      


Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln 
            20                  25                  30          


Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys 
        35                  40                  45              


Cys Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val 
    50                  55                  60                  


Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn 
65                  70                  75                  80  


Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser 
                85                  90                  95  


<210>  37
<211>  127
<212>  PRT
<213>  homo sapiens

<220>
<223>  wild type TCR beta domain

<400>  37

Asp Leu Lys Asn Val Phe Pro Pro Lys Val Ala Val Phe Glu Pro Ser 
1               5                   10                  15      


Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu Ala 
            20                  25                  30          


Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn Gly 
        35                  40                  45              


Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys Glu 
    50                  55                  60                  


Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu Arg 
65                  70                  75                  80  


Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys Gln 
                85                  90                  95      


Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp Arg 
            100                 105                 110         


Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125         
    


<210>  38
<211>  130
<212>  PRT
<213>  artificial sequence

<220>
<223>  4L4T_Beta_Crystal

<400>  38

Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro 
1               5                   10                  15      


Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  


Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Ala Leu Ser Ser Arg Leu 
65                  70                  75                  80  


Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      


Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         


Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             


Ala Asp 
    130 


<210>  39
<211>  95
<212>  PRT
<213>  artificial sequence

<220>
<223>  TRAC_Human

<400>  39

Pro Asn Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser 
1               5                   10                  15      


Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln 
            20                  25                  30          


Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys 
        35                  40                  45              


Thr Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val 
    50                  55                  60                  


Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn 
65                  70                  75                  80  


Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser 
                85                  90                  95  


<210>  40
<211>  95
<212>  PRT
<213>  artificial sequence

<220>
<223>  4L4T_Alpha_Crystal

<400>  40

Pro Asp Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser 
1               5                   10                  15      


Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln 
            20                  25                  30          


Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys 
        35                  40                  45              


Cys Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val 
    50                  55                  60                  


Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn 
65                  70                  75                  80  


Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser 
                85                  90                  95  


<210>  41
<211>  95
<212>  PRT
<213>  artificial sequence

<220>
<223>  E17_Design_2_QQQQ_IgG4

<400>  41

Pro Asp Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser 
1               5                   10                  15      


Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln 
            20                  25                  30          


Thr Gln Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys 
        35                  40                  45              


Cys Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val 
    50                  55                  60                  


Ala Trp Ser Gln Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Gln Asn 
65                  70                  75                  80  


Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser 
                85                  90                  95  


<210>  42
<211>  129
<212>  PRT
<213>  artificial sequence

<220>
<223>  TRBC1_Human

<400>  42

Glu Asp Leu Asn Lys Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro 
1               5                   10                  15      


Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Thr Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  


Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu 
65                  70                  75                  80  


Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      


Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         


Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             


Ala 
    


<210>  43
<211>  130
<212>  PRT
<213>  artificial sequence

<220>
<223>  4L4T_Beta_Crystal

<400>  43

Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu 
1               5                   10                  15      


Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys 
            20                  25                  30          


Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val 
        35                  40                  45              


Asn Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln Pro Leu 
    50                  55                  60                  


Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Ala Leu Ser Ser Arg 
65                  70                  75                  80  


Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg 
                85                  90                  95      


Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln 
            100                 105                 110         


Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly 
        115                 120                 125             


Arg Ala 
    130 


<210>  44
<211>  130
<212>  PRT
<213>  artificial sequence

<220>
<223>  E17_Design_2_QQQQ_IgG4

<400>  44

Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu 
1               5                   10                  15      


Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys 
            20                  25                  30          


Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val 
        35                  40                  45              


Asn Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln Pro Leu 
    50                  55                  60                  


Lys Glu Gln Pro Ala Leu Gln Asp Ser Arg Tyr Ala Leu Ser Ser Arg 
65                  70                  75                  80  


Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg 
                85                  90                  95      


Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln 
            100                 105                 110         


Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly 
        115                 120                 125             


Arg Ala 
    130 


<210>  45
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  45

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  46
<211>  17
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  46

Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly 
1               5                   10                  15      


Pro 
    


