                         SEQUENCE LISTING

<110>  COLD SPRING HARBOR LABORATORY
 
<120>  PROGRAMMABLE RNA-TEMPLATED SEQUENCING BY LIGATION (rSBL)

<130>  90418-A-PCT/GJG/DH

<150>  US 62/731,708
<151>  2018-09-14

<160>  213   

<170>  PatentIn version 3.5

<210>  1
<211>  33
<212>  RNA
<213>  Homo sapiens

<400>  1
auaaacuugu gguguggagc ugguggcgua ggc                                    33


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSBL primer for human KRAS G12 codon point mutation

<400>  2
agctccacac cacaagttta                                                   20


<210>  3
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expected wild-type rSBL product of human KRAS G12 codon

<400>  3
gcctacgcca ccagctccac accacaagtt ta                                     32


<210>  4
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  non-wild type complementary sequence for human KRAS G12 codon

<400>  4
gcctacgccb ddagctccac accacaagtt ta                                     32


<210>  5
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSBL probe for detection of human KRAS G12 codon point mutation

<400>  5
gcctacgccb cc                                                           12


<210>  6
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSBL probe for detection of human KRAS G12 codon point mutation

<400>  6
gcctacgcca dc                                                           12


<210>  7
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSBL probe for detection of human KRAS G12 codon point mutation

<400>  7
gcctacgcca cd                                                           12


<210>  8
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSBL probe for wild-type sequence of human KRAS G12 codon point 
       mutation

<400>  8
gcctacgcca cc                                                           12


<210>  9
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSBL primer for detecting G12A Kras mutations


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  PCR adapter sequence, in situ PCR adapter sequence, or in situ 
       signal amplification handle

<220>
<221>  misc_feature
<222>  (38)..(40)
<223>  phosphorothioate bond

<400>  9
agctccaact accacaagtt caggatacac actacccgtg                             40


<210>  10
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSBL probe for detecting G12A Kras mutation


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  PCR adapter sequence, in situ PCR adapter sequence, or in situ 
       signal amplification handle

<220>
<221>  misc_feature
<222>  (30)..(32)
<223>  n is a, c, g, or t

<400>  10
gggtcatatc ggtcactgtt gcctacgccn nn                                     32


<210>  11
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSBL probe for detecting G12A Kras mutation

<400>  11
gggtcatatc ggtcactgtt gcctacgccb cc                                     32


<210>  12
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSBL probe for detecting G12A Kras mutation


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  PCR adapter sequence, in situ PCR adapter sequence, or in situ 
       signal amplification handle

<400>  12
gggtcatatc ggtcactgtt gcctacgcca dc                                     32


<210>  13
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSBL probe for detecting G12A Kras mutation


<220>
<221>  misc_feature
<223>  PCR adapter sequence, in situ PCR adapter sequence, or in situ 
       signal amplification handle

<400>  13
gggtcatatc ggtcactgtt gcctacgcca cd                                     32


<210>  14
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSBL probe for detecting G12A Kras mutation

<400>  14
gcctacgcca cc                                                           12


<210>  15
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer for Q61K mutation

<400>  15
acctgctgtg tcgagaatat caggatacac actacccgtg                             40


<210>  16
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for Q61K mutation


<220>
<221>  misc_feature
<222>  (30)..(32)
<223>  n is a, c, g, or t

<400>  16
gggtcatatc ggtcactgtt gtactcctcn nn                                     32


<210>  17
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for Q61K mutation

<400>  17
gggtcatatc ggtcactgtt gtactcctcv tg                                     32


<210>  18
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for Q61K mutation

<400>  18
gggtcatatc ggtcactgtt gtactcctct vg                                     32


<210>  19
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for Q61K mutation

<400>  19
gggtcatatc ggtcactgtt gtactcctct th                                     32


<210>  20
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for Q61K mutation

<400>  20
gtactcctct tg                                                           12


<210>  21
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer for A146P mutation

<400>  21
tgatgtttca ataaaaggaa tcaggataca cactacccgt g                           41


<210>  22
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for A146P mutation


<220>
<221>  misc_feature
<222>  (30)..(32)
<223>  n is a, c, g, or t

<400>  22
gggtcatatc ggtcactgtt tcttgtcttn nn                                     32


<210>  23
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for A146P mutation

<400>  23
gggtcatatc ggtcactgtt tcttgtcttv gc                                     32


<210>  24
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for A146P mutation

<400>  24
gggtcatatc ggtcactgtt tcttgtcttt hc                                     32


<210>  25
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for A146P mutation

<400>  25
gggtcatatc ggtcactgtt tcttgtcttt gd                                     32


<210>  26
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for A146P mutation

<400>  26
tcttgtcttt gc                                                           12


<210>  27
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer for G13C mutation

<400>  27
accagctcca actaccacaa caggatacac actacccgtg                             40


<210>  28
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G13C mutation


<220>
<221>  misc_feature
<222>  (30)..(32)
<223>  n is a, c, g, or t

<400>  28
gggtcatatc ggtcactgtt cttgcctacn nn                                     32


<210>  29
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G13C mutation

<400>  29
gggtcatatc ggtcactgtt cttgcctacd gg                                     32


<210>  30
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G13C mutation

<400>  30
gggtcatatc ggtcactgtt cttgcctacc hg                                     32


<210>  31
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G13C mutation

<400>  31
gggtcatatc ggtcactgtt cttgcctacc gh                                     32


<210>  32
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G13C mutation

<400>  32
cttgcctacc gg                                                           12


<210>  33
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer for G12,13 (adjacent) mutation

<400>  33
agctccaact accacaagtt caggatacac actacccgtg                             40


<210>  34
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G12,13 (adjacent) mutation


<220>
<221>  misc_feature
<222>  (27)..(32)
<223>  n is a, c, g, or t

<400>  34
gggtcatatc ggtcactgtt gcctacnnnn nn                                     32


<210>  35
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G12,13 (adjacent) mutation

<400>  35
gggtcatatc ggtcactgtt gcctachcca cc                                     32


<210>  36
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G12,13 (adjacent) mutation

<400>  36
gggtcatatc ggtcactgtt gcctacgdca cc                                     32


<210>  37
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G12,13 (adjacent) mutation

<400>  37
gggtcatatc ggtcactgtt gcctacgcda cc                                     32


<210>  38
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G12,13 (adjacent) mutation

<400>  38
gggtcatatc ggtcactgtt gcctacgccb cc                                     32


<210>  39
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G12,13 (adjacent) mutation

<400>  39
gggtcatatc ggtcactgtt gcctacgcca dc                                     32


<210>  40
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G12,13 (adjacent) mutation

<400>  40
gggtcatatc ggtcactgtt gcctacgcca cd                                     32


<210>  41
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for G12,13 (adjacent) mutation

<400>  41
gcctacgcca cc                                                           12


<210>  42
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer of synthetic transcript

<400>  42
agatgggacc tacaatgtac cagaagcgtc tatgacactc                             40


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer of synthetic transcript

<400>  43
cgatcgtcta cttctattga                                                   20


<210>  44
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer of synthetic transcript

<400>  44
cgatcgatcg atcgtctact tctattgc                                          28


<210>  45
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer of synthetic transcript

<400>  45
gatcgatcga tcgatcgatc gtctacttct attgg                                  35


<210>  46
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer of synthetic transcript

<400>  46
atcgatcgat cgatcgatcg atcgatcgtc tacttctatt gt                          42


<210>  47
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic RNA transcript

<400>  47
gacgcuucug guacauugua ggucccaucu ucaauagaag u                           41


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  QPCR primer AP1

<400>  48
gggtcatatc ggtcactgtt                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  QPCR primer AP2

<400>  49
cacgggtagt gtgtatcctg                                                   20


<210>  50
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward sequencing primer (IDT)


<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  n is a, c, g, or t

<400>  50
gggtcatatc ggtcactgtt nnnnnnnnnn nn                                     32


<210>  51
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  51
agcagctgtg tccatgcagc caggatacac actacccgtg                             40


<210>  52
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  52
atgtttgtga tgtgcccggt caggatacac actacccgtg                             40


<210>  53
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  53
aaacataatc ctcaggtatc caggatacac actacccgtg                             40


<210>  54
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  54
aatgtgcttt caattgatgg caggatacac actacccgtg                             40


<210>  55
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  55
tactgttctt gaaataccta caggatacac actacccgtg                             40


<210>  56
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  56
acacacaatt catcaagcac caggatacac actacccgtg                             40


<210>  57
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  57
ttcctgaggt tctgcatcca caggatacac actacccgtg                             40


<210>  58
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  58
attctttcca gcattgtgct caggatacac actacccgtg                             40


<210>  59
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  59
tgaccccaat aaagtttctg caggatacac actacccgtg                             40


<210>  60
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  60
agcactgagg tgggggagca caggatacac actacccgtg                             40


<210>  61
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  61
acagtgaaga caagaatggt caggatacac actacccgtg                             40


<210>  62
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  62
tttgagcttt gaacactgaa caggatacac actacccgtg                             40


<210>  63
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  63
aaagaaaaac ccaatgtaaa caggatacac actacccgtg                             40


<210>  64
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  64
ttatttccaa atacttgaga caggatacac actacccgtg                             40


<210>  65
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  65
aaaaccacag ataaccaagg caggatacac actacccgtg                             40


<210>  66
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  66
tttagaagaa tgcctcctcg caggatacac actacccgtg                             40


<210>  67
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  67
ttgtactaac ttatgataga caggatacac actacccgtg                             40


<210>  68
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  68
aaggagcggg tgtgggagaa caggatacac actacccgtg                             40


<210>  69
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  69
agctgtgggc acattatgta caggatacac actacccgtg                             40


<210>  70
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  70
agtcctttga ggggacagat caggatacac actacccgtg                             40


<210>  71
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  71
tgcctgcagc cttcataagc caggatacac actacccgtg                             40


<210>  72
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  72
agccatggag tgggcactac caggatacac actacccgtg                             40


<210>  73
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  73
tcggttttgc cggtttcttt caggatacac actacccgtg                             40


<210>  74
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  74
acgcttcaat ttccttccat caggatacac actacccgtg                             40


<210>  75
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  75
aagctgccaa agaacacatc caggatacac actacccgtg                             40


<210>  76
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  76
atgtcatgaa agattttgag caggatacac actacccgtg                             40


<210>  77
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  77
tctgtccatg atgtcaaaag caggatacac actacccgtg                             40


<210>  78
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  78
tgtacactga ggtaggaaat caggatacac actacccgtg                             40


<210>  79
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  79
agcgcttggt ctgtgtcctt caggatacac actacccgtg                             40


<210>  80
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  80
tagctgcgac caacaacagc caggatacac actacccgtg                             40


<210>  81
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  81
aatcataaaa taacagtaaa caggatacac actacccgtg                             40


<210>  82
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  82
tcatccccaa gcccctcaag caggatacac actacccgtg                             40


<210>  83
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  83
accctcttaa atgtcaaaga caggatacac actacccgtg                             40


<210>  84
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  84
ttctcatcct ttctcgctct caggatacac actacccgtg                             40


<210>  85
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  85
tgccctgcac attttctttt caggatacac actacccgtg                             40


<210>  86
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  86
aagtaaagaa acgctttgta caggatacac actacccgtg                             40


<210>  87
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  87
aaagtgtcat caatttgtaa caggatacac actacccgtg                             40


<210>  88
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  88
agcttgggag ccacatggct caggatacac actacccgtg                             40


<210>  89
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  89
agcttgatca ccaccgcctt caggatacac actacccgtg                             40


<210>  90
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  90
tggccttcag aagcatctcc caggatacac actacccgtg                             40


<210>  91
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  91
aaatcacctg cagctaagca caggatacac actacccgtg                             40


<210>  92
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  92
aagtgggctt tttgtgaact caggatacac actacccgtg                             40


<210>  93
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  93
tggcagcagg gcctcgggaa caggatacac actacccgtg                             40


<210>  94
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  94
agaattaaaa agcttttttt caggatacac actacccgtg                             40


<210>  95
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  95
ttaacttgcc caaagttcac caggatacac actacccgtg                             40


<210>  96
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  96
aaaaagttgt aatagagaat caggatacac actacccgtg                             40


<210>  97
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  97
tccttgatgt caaaatgggg caggatacac actacccgtg                             40


<210>  98
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  98
agacccaccc ataaggctgc caggatacac actacccgtg                             40


<210>  99
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  99
acgccttctc caacaaaaga caggatacac actacccgtg                             40


<210>  100
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  100
accaacagac cccaatttcc caggatacac actacccgtg                             40


<210>  101
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  101
atcgggaccg agacctgctt caggatacac actacccgtg                             40


<210>  102
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  102
tttttaagag caaattctgt caggatacac actacccgtg                             40


<210>  103
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  103
ttagagatag agaaacagac caggatacac actacccgtg                             40


<210>  104
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  104
ttggtagtca tgtctttgtg caggatacac actacccgtg                             40


<210>  105
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  105
ttcttctctg ctgcatttgg caggatacac actacccgtg                             40


<210>  106
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  106
tcgctgtgtg aatgggcagt caggatacac actacccgtg                             40


<210>  107
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  107
acagagatgc agatggacgg caggatacac actacccgtg                             40


<210>  108
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  108
tgatgtccac actgctcggc caggatacac actacccgtg                             40


<210>  109
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  109
aaagtggtga ggagaaaaca caggatacac actacccgtg                             40


<210>  110
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  110
tgatggaagc agcggaggcc caggatacac actacccgtg                             40


<210>  111
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  111
agagcaaggc tgtagagatt caggatacac actacccgtg                             40


<210>  112
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  112
aactaaatcc cgaaatacaa caggatacac actacccgtg                             40


<210>  113
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  113
aggatagcct ttcagaccaa caggatacac actacccgtg                             40


<210>  114
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  114
acgccccacc tgccaccctc caggatacac actacccgtg                             40


<210>  115
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  115
agcactacca tgcagggtac caggatacac actacccgtg                             40


<210>  116
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  116
taaaagtgta ataatggaaa caggatacac actacccgtg                             40


<210>  117
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  117
tgctcgcagt gggctgattc caggatacac actacccgtg                             40


<210>  118
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  118
aggggagtga tttaagcaat caggatacac actacccgtg                             40


<210>  119
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  119
attctagagt ttggaatgca caggatacac actacccgtg                             40


<210>  120
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  120
actgtgctag gctatccaag caggatacac actacccgtg                             40


<210>  121
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  121
aaagtttata aacaaagctc caggatacac actacccgtg                             40


<210>  122
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  122
acactcacct tagggttcag caggatacac actacccgtg                             40


<210>  123
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  123
ttgacatcaa gaaaagacta caggatacac actacccgtg                             40


<210>  124
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  124
atccactggt actgcaggtt caggatacac actacccgtg                             40


<210>  125
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  125
tgactggccc acacgtgcat caggatacac actacccgtg                             40


<210>  126
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  126
aaccctgggt caaaaagaga caggatacac actacccgtg                             40


<210>  127
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  127
agtaggtgtg ggtaatttgg caggatacac actacccgtg                             40


<210>  128
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  128
aggatcagaa acagctttgg caggatacac actacccgtg                             40


<210>  129
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  129
agtgctttgt agtctctcct caggatacac actacccgtg                             40


<210>  130
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  130
tccctgacac tttaacctca caggatacac actacccgtg                             40


<210>  131
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  131
tagacacatt ctttattatt caggatacac actacccgtg                             40


<210>  132
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  132
ttctcagtgt ccacagcgca caggatacac actacccgtg                             40


<210>  133
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  133
tttaatatgc ttataaccta caggatacac actacccgtg                             40


<210>  134
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  134
ttaaagaccc tgagttatgt caggatacac actacccgtg                             40


<210>  135
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  135
aacttcttac cctaaaagag caggatacac actacccgtg                             40


<210>  136
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  136
ttttaaaata ctagcctgta caggatacac actacccgtg                             40


<210>  137
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  137
aaactcagga aaacctctca caggatacac actacccgtg                             40


<210>  138
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  138
ttctctcccg tatcacctaa caggatacac actacccgtg                             40


<210>  139
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  139
aaaaaaaaaa tggaaaaaaa caggatacac actacccgtg                             40


<210>  140
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  140
aaaaacaagc tggataacca caggatacac actacccgtg                             40


<210>  141
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  141
tggcttgtct tttttcacca caggatacac actacccgtg                             40


<210>  142
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  142
tctcagtgtt tcaacctcac caggatacac actacccgtg                             40


<210>  143
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  143
aaagtctcgt gcacatgtga caggatacac actacccgtg                             40


<210>  144
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  144
tgatggtgct cagattgcaa caggatacac actacccgtg                             40


<210>  145
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  145
tgcaacaaaa gattagaaca caggatacac actacccgtg                             40


<210>  146
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  146
ttagtatata gaaataatac caggatacac actacccgtg                             40


<210>  147
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer for KRAS G12D mutation for in situ RCA

<400>  147
agctccaacc accacaagtt tatactcagt cattttctcg ggaa                        44


<210>  148
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for KRAS G12V mutation for in situ RCA

<400>  148
cgctgaagat acgccacc                                                     18


<210>  149
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for KRAS G12V mutation for in situ RCA

<400>  149
cgctgaagat acgccadc                                                     18


<210>  150
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for KRAS G12V mutation for in situ RCA

<400>  150
cgctgaagat acgccacd                                                     18


<210>  151
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for KRAS G12V mutation for in situ RCA

<400>  151
cgctgaagag cctacgcc                                                     18


<210>  152
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer for TP53 R273H mutation for in situ RCA

<400>  152
cacctcaaag ctgttccgtc ccagtagatc tcgggaa                                37


<210>  153
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for TP53 R273C mutation for in situ RCA

<400>  153
cgctgaagac acaaacacg                                                    19


<210>  154
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for TP53 R273C mutation for in situ RCA

<400>  154
cgctgaagac acaaacadg                                                    19


<210>  155
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for TP53 R273C mutation for in situ RCA

<400>  155
cgctgaagac acaaacach                                                    19


<210>  156
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for TP53 R273C mutation for in situ RCA

<400>  156
cgctgaagaa ggcacaaac                                                    19


<210>  157
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer for TP53 T220C mutation for in situ RCA

<400>  157
gggcaccacc acactatgtc gaaaagtctc gggaa                                  35


<210>  158
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for TP53 T220C mutation for in situ RCA

<400>  158
cgctgaagag cggctcata                                                    19


<210>  159
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for TP53 T220C mutation for in situ RCA

<400>  159
cgctgaagag cggctcava                                                    19


<210>  160
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for TP53 T220C mutation for in situ RCA

<400>  160
cgctgaagag cggctcatb                                                    19


<210>  161
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for TP53 T220C mutation for in situ RCA

<400>  161
cgctgaagac aggcggctc                                                    19


<210>  162
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing primer for ProRSBL nucleotide bias determination

<400>  162
cgatgggacc tacaatgtac cagaagcgtc tctatgacac tc                          42


<210>  163
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing primer for ProRSBL nucleotide bias determination

<400>  163
ggatgggacc tacaatgtac cagaagcgtc tctctatgac actc                        44


<210>  164
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing primer for ProRSBL nucleotide bias determination

<400>  164
tgatgggacc tacaatgtac cagaagcgtc tctctctatg acactc                      46


<210>  165
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Competitive probe for ProRSBL nucleotide bias determination

<400>  165
cgatcgtcta cttctattga                                                   20


<210>  166
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Competitive probe for ProRSBL nucleotide bias determination

<400>  166
cgatcgatcg atcgtctact tctattgc                                          28


<210>  167
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  167
cgatcgatcg atcgatcgat cgtctacttc tattgg                                 36


<210>  168
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  168
gacgcuucug guacauugua ggucccaucu gcaauagaag ua                          42


<210>  169
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  169
gacgcuucug guacauugua ggucccaucu ccaauagaag ua                          42


<210>  170
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  170
gacgcuucug guacauugua ggucccaucu acaauagaag ua                          42


<210>  171
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  171
gacgcuucug guacauugua ggucccauca ucaauagaag ua                          42


<210>  172
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  172
gacgcuucug guacauugua ggucccauca gcaauagaag ua                          42


<210>  173
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  173
gacgcuucug guacauugua ggucccauca ccaauagaag ua                          42


<210>  174
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  174
gacgcuucug guacauugua ggucccauca acaauagaag ua                          42


<210>  175
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  175
gacgcuucug guacauugua ggucccaucg ucaauagaag ua                          42


<210>  176
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  176
gacgcuucug guacauugua ggucccaucg gcaauagaag ua                          42


<210>  177
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  177
gacgcuucug guacauugua ggucccaucg ccaauagaag ua                          42


<210>  178
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  178
gacgcuucug guacauugua ggucccaucg acaauagaag ua                          42


<210>  179
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  179
gacgcuucug guacauugua ggucccaucc ucaauagaag ua                          42


<210>  180
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  180
gacgcuucug guacauugua ggucccaucc gcaauagaag ua                          42


<210>  181
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  181
gacgcuucug guacauugua ggucccaucc ccaauagaag ua                          42


<210>  182
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA template for ProRSBL nucleotide bias determination

<400>  182
gacgcuucug guacauugua ggucccaucc acaauagaag ua                          42


<210>  183
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Reverse sequencing RNA

<400>  183
gacgauucug guucauugua ggucccaucu ucaauagaag ua                          42


<210>  184
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forwarding sequencing primer

<400>  184
agatgggacc tacaatgtac cagaagcgtc tatgacactc gatcaggata cacactaccc       60

gtg                                                                     63


<210>  185
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse sequencing primer

<400>  185
ttgggtcata tcggtcactg tttatgacac tctacttcta ttgaagatgg gacctacaat       60

ga                                                                      62


<210>  186
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward ProRSBL probe 1-4


<220>
<221>  misc_feature
<222>  (39)..(42)
<223>  n is a, c, g, or t

<400>  186
ttgggtcata tcggtcactg ttcgatcgtc tacttctann nn                          42


<210>  187
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward ProRSBL probe 5-8


<220>
<221>  misc_feature
<222>  (35)..(38)
<223>  n is a, c, g, or t

<400>  187
ttgggtcata tcggtcactg ttcgatcgtc tactnnnntt ga                          42


<210>  188
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward ProRSBL probe 9-12


<220>
<221>  misc_feature
<222>  (31)..(34)
<223>  n is a, c, g, or t

<400>  188
ttgggtcata tcggtcactg ttcgatcgtc nnnntctatt ga                          42


<210>  189
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse ProRSBL probe 1-4


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, or t

<400>  189
nnnngaatcg tccgatcgtc gatcaggata cacactaccc gtg                         43


<210>  190
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse ProRSBL probe 9-12


<220>
<221>  misc_feature
<222>  (5)..(8)
<223>  n is a, c, g, or t

<400>  190
accannnncg tccgatcgtc gatcaggata cacactaccc gtg                         43


<210>  191
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse ProRSBL probe 9-12


<220>
<221>  misc_feature
<222>  (9)..(12)
<223>  n is a, c, g, or t

<400>  191
accagaatnn nncgatcgtc gatcaggata cacactaccc gg                          42


<210>  192
<211>  52
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic RNA (from human 28S rRNA 4490-4540)

<400>  192
cgugagcugg guuuagaccg ucgugagaca gguuaguuuu acccuacuga ug               52


<210>  193
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cleavage substrate I3 (for cleavage kinetics)

<400>  193
gtaaaacaac ctgtctcacg acggtctaaa cccagctcac                             40


<210>  194
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cleavage substrate I8 (for post cleavage ligation)

<400>  194
gctagctagc tagctagcag gtaaaacta                                         29


<210>  195
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ligation probe A

<400>  195
tagctagcta gctagctagc aggtaaaact c                                      31


<210>  196
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ligation probe C

<400>  196
gctagctagc tagctagcta gcaggtaaaa ctg                                    33


<210>  197
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ligation probe G

<400>  197
gctagctagc tagctagcta gcaggtaaaa ctg                                    33


<210>  198
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ligation probe T

<400>  198
ctgctagcta gctagctagc tagcaggtaa aactt                                  35


<210>  199
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer for IDH1 mutant detection

<400>  199
cctatgatga taggttttac ccatccactc acaatctcgg gaa                         43


<210>  200
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for IDH1 mutant detection

<400>  200
cgctgaagaa agcatgach                                                    19


<210>  201
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for IDH1 mutant detection

<400>  201
cgctgaagaa agcatgadg                                                    19


<210>  202
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for IDH1 mutant detection

<400>  202
cgctgaagaa agcatgbcg                                                    19


<210>  203
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for IDH1 mutant detection

<400>  203
cgctgaagaa agcatgacg                                                    19


<210>  204
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic template for KRAS mutant detection

<400>  204
aacuuguggu aguuggagcu gguggcguag gc                                     32


<210>  205
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic template for KRAS mutant detection

<400>  205
aacuuguggu aguuggagcu gauggcguag gc                                     32


<210>  206
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic template for KRAS mutant detection

<400>  206
aacuuguggu aguuggagcu guuggcguag gc                                     32


<210>  207
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic template for KRAS mutant detection

<400>  207
aacuuguggu aguuggagcu cguggcguag gc                                     32


<210>  208
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL primer for KRAS mutant detection

<400>  208
agctccaacc accacaagtt tatactcagt catttcagga tacacactac ccgtg            55


<210>  209
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for KRAS mutant detection

<400>  209
gggtcatatc ggtcactgtt tacgccacd                                         29


<210>  210
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for KRAS mutant detection

<400>  210
gggtcatatc ggtcactgtt tacgccadc                                         29


<210>  211
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe for KRAS mutant detection

<400>  211
gggtcatatc ggtcactgtt tacgccbcc                                         29


<210>  212
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL probe

<400>  212
gcctacgcca cd                                                           12


<210>  213
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SBL Probe

<400>  213
gcctacgcca dc                                                           12


