                         SEQUENCE LISTING

<110>  Vestaron Corporation
       Kennedy, Robert M.
       Bao, Lin
 
<120>  AV3 MUTANT INSECTICIDAL POLYPEPTIDES AND METHODS FOR PRODUCING 
       AND USING SAME

<130>  225312-454979

<150>  62/731,387
<151>  2018-09-14

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  PRT
<213>  Anemonia viridis

<400>  1

Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly Gln Asn 
1               5                   10                  15      


Cys Tyr Pro Glu Gly Cys Ser Gly Pro Lys Val 
            20                  25          


<210>  2
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal amino acid substitution of R1K relative to SEQ ID NO: 
       1

<400>  2

Lys Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly Gln Asn 
1               5                   10                  15      


Cys Tyr Pro Glu Gly Cys Ser Gly Pro Lys Val 
            20                  25          


<210>  3
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal amino acid deletion relative to SEQ ID NO: 1

<400>  3

Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly Gln Asn 
1               5                   10                  15      


Cys Tyr Pro Glu Gly Cys Ser Gly Pro Lys 
            20                  25      


<210>  4
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal amino acid substitution of R1K and C-terminal amino 
       acid deletion

<400>  4

Lys Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly Gln Asn 
1               5                   10                  15      


Cys Tyr Pro Glu Gly Cys Ser Gly Pro Lys 
            20                  25      


<210>  5
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Barley Alpha-Amylase Signal peptide (BAAS)

<400>  5

Met Ala Asn Lys His Leu Ser Leu Ser Leu Phe Leu Val Leu Leu Gly 
1               5                   10                  15      


Leu Ser Ala Ser Leu Ala Ser Gly 
            20                  


<210>  6
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavable type of intervening linker

<400>  6

Ile Gly Glu Arg 
1               


<210>  7
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Intervening linker peptide

<400>  7

Glu Glu Lys Lys Asn 
1               5   


<210>  8
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Uncleavable intervening linker peptide

<400>  8

Glu Thr Met Phe Lys His Gly Leu 
1               5               


<210>  9
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Green-Fluorescent Protein (GFP)

<400>  9

Ala Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  10
<211>  157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Translational stabilizing protein

<400>  10

Met Ala Lys Ala Ser Leu Leu Ile Leu Ala Thr Ile Phe Leu Gly Val 
1               5                   10                  15      


Ile Thr Pro Ser Cys Leu Ser Glu Asn Ile Leu Tyr Ser Gly Glu Thr 
            20                  25                  30          


Leu Pro Thr Gly Gly Phe Leu Ser Ser Gly Ser Phe Val Phe Ile Met 
        35                  40                  45              


Gln Glu Asp Cys Asn Leu Val Leu Tyr Asn Val Asp Lys Pro Ile Trp 
    50                  55                  60                  


Ala Thr Asn Thr Gly Gly Leu Ser Ser Asp Cys Ser Leu Ser Met Gln 
65                  70                  75                  80  


Asn Asp Gly Asn Leu Val Val Phe Thr Pro Ser Asn Lys Pro Ile Trp 
                85                  90                  95      


Ala Ser Asn Thr Asp Gly Gln Asn Gly Asn Tyr Val Cys Ile Leu Gln 
            100                 105                 110         


Lys Asp Arg Asn Val Val Ile Tyr Gly Thr Asn Arg Trp Ala Thr Gly 
        115                 120                 125             


Thr Tyr Thr Gly Ala Val Gly Ile Pro Glu Ser Pro Pro Ser Glu Lys 
    130                 135                 140                 


Tyr Pro Ser Ala Gly Lys Ile Lys Leu Val Thr Ala Lys 
145                 150                 155         


<210>  11
<211>  224
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Translational stabilizing protein

<400>  11

Lys Phe Asp Ile Lys Asn Gln Cys Gly Tyr Thr Val Trp Ala Ala Gly 
1               5                   10                  15      


Leu Pro Gly Gly Gly Lys Arg Leu Asp Gln Gly Gln Thr Trp Thr Val 
            20                  25                  30          


Asn Leu Ala Ala Gly Thr Ala Ser Ala Arg Phe Trp Gly Arg Thr Gly 
        35                  40                  45              


Cys Thr Phe Asp Ala Ser Gly Lys Gly Ser Cys Gln Thr Gly Asp Cys 
    50                  55                  60                  


Gly Gly Gln Leu Ser Cys Thr Val Ser Gly Ala Val Pro Ala Thr Leu 
65                  70                  75                  80  


Ala Glu Tyr Thr Gln Ser Asp Gln Asp Tyr Tyr Asp Val Ser Leu Val 
                85                  90                  95      


Asp Gly Phe Asn Ile Pro Leu Ala Ile Asn Pro Thr Asn Ala Gln Cys 
            100                 105                 110         


Thr Ala Pro Ala Cys Lys Ala Asp Ile Asn Ala Val Cys Pro Ser Glu 
        115                 120                 125             


Leu Lys Val Asp Gly Gly Cys Asn Ser Ala Cys Asn Val Phe Lys Thr 
    130                 135                 140                 


Asp Gln Tyr Cys Cys Arg Asn Ala Tyr Val Asp Asn Cys Pro Ala Thr 
145                 150                 155                 160 


Asn Tyr Ser Lys Ile Phe Lys Asn Gln Cys Pro Gln Ala Tyr Ser Tyr 
                165                 170                 175     


Ala Lys Asp Asp Thr Ala Thr Phe Ala Cys Ala Ser Gly Thr Asp Tyr 
            180                 185                 190         


Ser Ile Val Phe Cys Met Ala Arg Val Ser Glu Leu Ala Phe Leu Leu 
        195                 200                 205             


Ala Ala Thr Leu Ala Ile Ser Leu His Met Gln Glu Ala Gly Val Val 
    210                 215                 220                 


<210>  12
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ESRP

<400>  12

Glu Met Gly Lys Met Ala Ser Leu Phe Ala Ser Leu Leu Val Val Leu 
1               5                   10                  15      


Val Ser Leu Ser Leu Ala Ser Glu Ser Ser Ala 
            20                  25          


<210>  13
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ESRP

<400>  13

Met Gly Lys Met Ala Ser Leu Phe Ala Thr Phe Leu Val Val Leu Val 
1               5                   10                  15      


Ser Leu Ser Leu Ala Ser Glu Ser Ser Ala 
            20                  25      


<210>  14
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding tobacco extensin signal peptide variant 1

<400>  14
atgggtaaga tggcttctct gtttgcttct ctgctggttg ttctggtttc tctgtctctg       60

gcttctgaat cttctgct                                                     78


<210>  15
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding tobacco extensin signal peptide variant 2

<400>  15
atgggtaaga tggcttctct gtttgctact tttctggttg ttctggtttc tctgtctctg       60

gcttctgaat cttctgct                                                     78


<210>  16
<211>  224
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Translational stabilizing protein

<400>  16

Met Ala Arg Val Ser Glu Leu Ala Phe Leu Leu Ala Ala Thr Leu Ala 
1               5                   10                  15      


Ile Ser Leu His Met Gln Glu Ala Gly Val Val Lys Phe Asp Ile Lys 
            20                  25                  30          


Asn Gln Cys Gly Tyr Thr Val Trp Ala Ala Gly Leu Pro Gly Gly Gly 
        35                  40                  45              


Lys Arg Leu Asp Gln Gly Gln Thr Trp Thr Val Asn Leu Ala Ala Gly 
    50                  55                  60                  


Thr Ala Ser Ala Arg Phe Trp Gly Arg Thr Gly Cys Thr Phe Asp Ala 
65                  70                  75                  80  


Ser Gly Lys Gly Ser Cys Gln Thr Gly Asp Cys Gly Gly Gln Leu Ser 
                85                  90                  95      


Cys Thr Val Ser Gly Ala Val Pro Ala Thr Leu Ala Glu Tyr Thr Gln 
            100                 105                 110         


Ser Asp Gln Asp Tyr Tyr Asp Val Ser Leu Val Asp Gly Phe Asn Ile 
        115                 120                 125             


Pro Leu Ala Ile Asn Pro Thr Asn Ala Gln Cys Thr Ala Pro Ala Cys 
    130                 135                 140                 


Lys Ala Asp Ile Asn Ala Val Cys Pro Ser Glu Leu Lys Val Asp Gly 
145                 150                 155                 160 


Gly Cys Asn Ser Ala Cys Asn Val Phe Lys Thr Asp Gln Tyr Cys Cys 
                165                 170                 175     


Arg Asn Ala Tyr Val Asp Asn Cys Pro Ala Thr Asn Tyr Ser Lys Ile 
            180                 185                 190         


Phe Lys Asn Gln Cys Pro Gln Ala Tyr Ser Tyr Ala Lys Asp Asp Thr 
        195                 200                 205             


Ala Thr Phe Ala Cys Ala Ser Gly Thr Asp Tyr Ser Ile Val Phe Cys 
    210                 215                 220                 


<210>  17
<211>  105
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Translational stabilizing protein

<400>  17

Asp Asn Ile Leu Tyr Ser Gly Glu Thr Leu Ser Thr Gly Glu Phe Leu 
1               5                   10                  15      


Asn Tyr Gly Ser Phe Val Phe Ile Met Gln Glu Asp Cys Asn Leu Val 
            20                  25                  30          


Leu Tyr Asp Val Asp Lys Pro Ile Trp Ala Thr Asn Thr Gly Gly Leu 
        35                  40                  45              


Ser Arg Ser Cys Phe Leu Ser Met Gln Thr Asp Gly Asn Leu Val Val 
    50                  55                  60                  


Tyr Asn Pro Ser Asn Lys Pro Ile Trp Ala Ser Asn Thr Gly Gly Gln 
65                  70                  75                  80  


Asn Gly Asn Tyr Val Cys Ile Leu Gln Lys Asp Arg Asn Val Val Ile 
                85                  90                  95      


Tyr Gly Thr Asp Arg Trp Ala Thr Gly 
            100                 105 


<210>  18
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Av3+2 peptide sequence ("Peak 2")

<400>  18

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Pro Lys Val 
            20                  25                  


<210>  19
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Av3+2 peptide with C-terminal valine cleaved ("Peak 1")

<400>  19

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Pro Lys 
            20                  25              


<210>  20
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutation strategy strain

<400>  20

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Pro Ala Val 
            20                  25                  


<210>  21
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutation strategy strain

<400>  21

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Ala Ala Val 
            20                  25                  


<210>  22
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutation strategy strain

<400>  22

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Pro His Val 
            20                  25                  


<210>  23
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutation strategy strain

<400>  23

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Ala His Val 
            20                  25                  


<210>  24
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutation strategy strain

<400>  24

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Pro Asp Val 
            20                  25                  


<210>  25
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutation strategy strain

<400>  25

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Pro Lys 
            20                  25              


<210>  26
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutation strategy strain

<400>  26

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Pro Lys Thr 
            20                  25                  


<210>  27
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutation strategy strain

<400>  27

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Pro Lys Ala 
            20                  25                  


<210>  28
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutation strategy strain

<400>  28

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Pro Lys Pro 
            20                  25                  


<210>  29
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutation strategy strain

<400>  29

Gly Ser Arg Ser Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly 
1               5                   10                  15      


Gln Asn Cys Tyr Pro Glu Gly Cys Ser Gly Pro Gln Val 
            20                  25                  


<210>  30
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pharmacophore mutant

<400>  30

Lys Ala Cys Cys Pro Cys Tyr Trp Gly Gly Cys Pro Trp Gly Ala Ala 
1               5                   10                  15      


Cys Tyr Pro Ala Gly Cys Ala Ala Ala Lys 
            20                  25      


<210>  31
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pharmacophore mutant

<400>  31

Lys Ala Cys Cys Pro Cys Tyr Trp Ala Ala Cys Pro Trp Ala Ala Ala 
1               5                   10                  15      


Cys Tyr Ala Ala Ala Cys Ala Ala Ala Lys 
            20                  25      


<210>  32
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pharmacophore mutant

<400>  32

Lys Pro Tyr Trp Pro Trp Tyr Lys 
1               5               


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pharmacophore mutant

<400>  33

Lys Pro Tyr Trp Pro Trp Tyr Lys Val 
1               5                   


<210>  34
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pharmacophore mutant

<400>  34

Pro Tyr Trp Pro Trp Tyr 
1               5       


