                         SEQUENCE LISTING

<110>  Pebble Labs USA Inc.
 
<120>  IMPROVED FLUORESCENT RESONANCE ENERGY TRANSFER-BASED BIOSENSOR 
       PROTEINS AND THEIR METHODS OF USE THEREOF

<130>  90115.00271

<150>  62/730,424
<151>  2018-09-12

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  2484
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TqLPNGh biosensor protein

<400>  1
catatggtat caaagggtga agaacttttc accggtgttg tgccaatttt ggtcgagtta       60

gacggtgatg tgaatggcca caaattctct gtctccggcg aaggtgaggg tgatgcaacc      120

tatggcaaac tgacgctgaa atttatttgc accaccggga agcttcccgt tccttggcct      180

actttggtta ccaccttgtc ttggggtgtg cagtgcttcg cacgttatcc tgaccacatg      240

aaacagcacg atttttttaa aagtgcaatg ccggaaggct atgtccagga acgtactatt      300

ttttttaagg atgatggcaa ctataaaacc cgtgccgagg tcaaatttga aggcgacacc      360

ttggttaacc gaattgaact gaaaggtatt gattttaaag aagatggtaa tattctgggt      420

cacaaattgg agtataacta tttcagcgac aacgtttata ttaccgcaga taaacaaaaa      480

aacggtatca aagccaactt taaaattaga cacaatatcg aagatggcgg tgtccaatta      540

gccgaccact atcagcagaa cacgccgatc ggtgatggtc ctgtgctgct gccggataat      600

cattaccttt caacacagag caaactgagc aaagacccga acgagaaacg tgaccacatg      660

gtactgctgg aatttgtgac agctgcgggc attacgcttg ggatggacga gctatataaa      720

acggccgggg gtgtgcttaa tggctattgg ggctatcagg agtttctgga tgagttccca      780

gaacagcgca atcttacgaa cgccctgtct gaagcggttc gggcgcagcc tgtgcctctg      840

tctaagccga ctcaacgtcc tatcaagatt tcggtcgtat accctgggca gcaagtttcc      900

gattactggg tgcgtaacat cgcaagcttt gaaaaacgtc tgtataaact gaacattaat      960

taccaactga accaagtgtt cacccgaccc aatgccgata ttaaacaaca gtcactcagt     1020

ctgatggagg ccctgaaatc caaatcggac tacctgatct ttaccttgga tacgactcgc     1080

caccgtaaat ttgtggagca cgtactcgat agtacaaata cgaaactgat tttacagaat     1140

ataacgacgc cggtacggga atgggacaaa caccagccgt tcctgtatgt gggttttgac     1200

catgctgaag gttcccgaga actggcaacc gaattcggta aatttttccc gaaacatacg     1260

tattactctg tgctctattt ctcggaaggc tacatcagcg atgtacgtgg agataccttt     1320

atccatcagg tgaatcggga taataacttt gagcttcagt ccgcttacta caccaaggcg     1380

accaagcaat ccggttatga tgctgccaag gcgagcttgg caaagcaccc tgacgttgac     1440

tttatttatg cctgctcgac ggacgtagct ctgggtgcag tagatgcact ggcagagctc     1500

gggagagagg atattatgat caacggttgg ggaggggggt ccgcggaatt agacgcgata     1560

cagaaaggtg atctggatat taccgtcatg cgtatgaacg atgacacggg tattgccatg     1620

gcagaagcga ttaaatggga tctggaagac aaaccagtac cgaccgtata tagtggcgac     1680

tttgaaatcg taaccaaagc ggattccccg gaacgaatcg aagcgctgaa gaagcgtgct     1740

tttcgctatt ccgataacgg tggggcggct gccatggcaa gccttccggc tacccatgag     1800

cttcatattt tcggcagtat taatggcgta gactttgaca tggtgggtca gggaaccgga     1860

aatcccaatg atggctatga agagttgaac ctgaaatcta ccaagggcga cctccagttt     1920

tcaccttgga ttttggtgcc acacatcggt tatggatttc atcagtattt accttatccg     1980

gatggaatga gtccgttcca ggcggccatg gttgatggtt ccggttatca ggtccatcgc     2040

acgatgcagt tcgaggacgg ggcctctctg acggtgaatt atcgttatac ctatgaaggt     2100

tcacatataa aaggtgaagc tcaggtaaaa ggcacgggtt ttccggcgga tggcccggtg     2160

atgaccaact ccctcacggc ggcggactgg tgccggagca aaaagacgta tccgaatgat     2220

aaaaccatca ttagcacctt taaatggtca tacacgactg gcaacggtaa acgctacaga     2280

tccaccgctc gcaccacata tacttttgct aaacctatgg ccgcaaacta tttgaaaaac     2340

cagcctatgt acgttttccg taaaaccgaa ctgaagcaca gtaaaactga actgaatttc     2400

aaagaatggc aaaaagcatt tacggatgtt atgggtatgg atgagctgta taagcatcac     2460

catcatcatc attaatagct cgag                                            2484


<210>  2
<211>  823
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TqLPNGh biosensor protein

<400>  2

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Ser Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Phe Ser Asp Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Thr 
225                 230                 235                 240 


Ala Gly Gly Val Leu Asn Gly Tyr Trp Gly Tyr Gln Glu Phe Leu Asp 
                245                 250                 255     


Glu Phe Pro Glu Gln Arg Asn Leu Thr Asn Ala Leu Ser Glu Ala Val 
            260                 265                 270         


Arg Ala Gln Pro Val Pro Leu Ser Lys Pro Thr Gln Arg Pro Ile Lys 
        275                 280                 285             


Ile Ser Val Val Tyr Pro Gly Gln Gln Val Ser Asp Tyr Trp Val Arg 
    290                 295                 300                 


Asn Ile Ala Ser Phe Glu Lys Arg Leu Tyr Lys Leu Asn Ile Asn Tyr 
305                 310                 315                 320 


Gln Leu Asn Gln Val Phe Thr Arg Pro Asn Ala Asp Ile Lys Gln Gln 
                325                 330                 335     


Ser Leu Ser Leu Met Glu Ala Leu Lys Ser Lys Ser Asp Tyr Leu Ile 
            340                 345                 350         


Phe Thr Leu Asp Thr Thr Arg His Arg Lys Phe Val Glu His Val Leu 
        355                 360                 365             


Asp Ser Thr Asn Thr Lys Leu Ile Leu Gln Asn Ile Thr Thr Pro Val 
    370                 375                 380                 


Arg Glu Trp Asp Lys His Gln Pro Phe Leu Tyr Val Gly Phe Asp His 
385                 390                 395                 400 


Ala Glu Gly Ser Arg Glu Leu Ala Thr Glu Phe Gly Lys Phe Phe Pro 
                405                 410                 415     


Lys His Thr Tyr Tyr Ser Val Leu Tyr Phe Ser Glu Gly Tyr Ile Ser 
            420                 425                 430         


Asp Val Arg Gly Asp Thr Phe Ile His Gln Val Asn Arg Asp Asn Asn 
        435                 440                 445             


Phe Glu Leu Gln Ser Ala Tyr Tyr Thr Lys Ala Thr Lys Gln Ser Gly 
    450                 455                 460                 


Tyr Asp Ala Ala Lys Ala Ser Leu Ala Lys His Pro Asp Val Asp Phe 
465                 470                 475                 480 


Ile Tyr Ala Cys Ser Thr Asp Val Ala Leu Gly Ala Val Asp Ala Leu 
                485                 490                 495     


Ala Glu Leu Gly Arg Glu Asp Ile Met Ile Asn Gly Trp Gly Gly Gly 
            500                 505                 510         


Ser Ala Glu Leu Asp Ala Ile Gln Lys Gly Asp Leu Asp Ile Thr Val 
        515                 520                 525             


Met Arg Met Asn Asp Asp Thr Gly Ile Ala Met Ala Glu Ala Ile Lys 
    530                 535                 540                 


Trp Asp Leu Glu Asp Lys Pro Val Pro Thr Val Tyr Ser Gly Asp Phe 
545                 550                 555                 560 


Glu Ile Val Thr Lys Ala Asp Ser Pro Glu Arg Ile Glu Ala Leu Lys 
                565                 570                 575     


Lys Arg Ala Phe Arg Tyr Ser Asp Asn Gly Gly Ala Ala Ala Met Ala 
            580                 585                 590         


Ser Leu Pro Ala Thr His Glu Leu His Ile Phe Gly Ser Ile Asn Gly 
        595                 600                 605             


Val Asp Phe Asp Met Val Gly Gln Gly Thr Gly Asn Pro Asn Asp Gly 
    610                 615                 620                 


Tyr Glu Glu Leu Asn Leu Lys Ser Thr Lys Gly Asp Leu Gln Phe Ser 
625                 630                 635                 640 


Pro Trp Ile Leu Val Pro His Ile Gly Tyr Gly Phe His Gln Tyr Leu 
                645                 650                 655     


Pro Tyr Pro Asp Gly Met Ser Pro Phe Gln Ala Ala Met Val Asp Gly 
            660                 665                 670         


Ser Gly Tyr Gln Val His Arg Thr Met Gln Phe Glu Asp Gly Ala Ser 
        675                 680                 685             


Leu Thr Val Asn Tyr Arg Tyr Thr Tyr Glu Gly Ser His Ile Lys Gly 
    690                 695                 700                 


Glu Ala Gln Val Lys Gly Thr Gly Phe Pro Ala Asp Gly Pro Val Met 
705                 710                 715                 720 


Thr Asn Ser Leu Thr Ala Ala Asp Trp Cys Arg Ser Lys Lys Thr Tyr 
                725                 730                 735     


Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys Trp Ser Tyr Thr Thr 
            740                 745                 750         


Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg Thr Thr Tyr Thr Phe 
        755                 760                 765             


Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn Gln Pro Met Tyr Val 
    770                 775                 780                 


Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr Glu Leu Asn Phe Lys 
785                 790                 795                 800 


Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly Met Asp Glu Leu Tyr 
                805                 810                 815     


Lys His His His His His His 
            820             


<210>  3
<211>  817
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TqLPNGh biosensor protein lacking H6 sequence at C-terminal end

<400>  3

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Ser Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Phe Ser Asp Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Thr 
225                 230                 235                 240 


Ala Gly Gly Val Leu Asn Gly Tyr Trp Gly Tyr Gln Glu Phe Leu Asp 
                245                 250                 255     


Glu Phe Pro Glu Gln Arg Asn Leu Thr Asn Ala Leu Ser Glu Ala Val 
            260                 265                 270         


Arg Ala Gln Pro Val Pro Leu Ser Lys Pro Thr Gln Arg Pro Ile Lys 
        275                 280                 285             


Ile Ser Val Val Tyr Pro Gly Gln Gln Val Ser Asp Tyr Trp Val Arg 
    290                 295                 300                 


Asn Ile Ala Ser Phe Glu Lys Arg Leu Tyr Lys Leu Asn Ile Asn Tyr 
305                 310                 315                 320 


Gln Leu Asn Gln Val Phe Thr Arg Pro Asn Ala Asp Ile Lys Gln Gln 
                325                 330                 335     


Ser Leu Ser Leu Met Glu Ala Leu Lys Ser Lys Ser Asp Tyr Leu Ile 
            340                 345                 350         


Phe Thr Leu Asp Thr Thr Arg His Arg Lys Phe Val Glu His Val Leu 
        355                 360                 365             


Asp Ser Thr Asn Thr Lys Leu Ile Leu Gln Asn Ile Thr Thr Pro Val 
    370                 375                 380                 


Arg Glu Trp Asp Lys His Gln Pro Phe Leu Tyr Val Gly Phe Asp His 
385                 390                 395                 400 


Ala Glu Gly Ser Arg Glu Leu Ala Thr Glu Phe Gly Lys Phe Phe Pro 
                405                 410                 415     


Lys His Thr Tyr Tyr Ser Val Leu Tyr Phe Ser Glu Gly Tyr Ile Ser 
            420                 425                 430         


Asp Val Arg Gly Asp Thr Phe Ile His Gln Val Asn Arg Asp Asn Asn 
        435                 440                 445             


Phe Glu Leu Gln Ser Ala Tyr Tyr Thr Lys Ala Thr Lys Gln Ser Gly 
    450                 455                 460                 


Tyr Asp Ala Ala Lys Ala Ser Leu Ala Lys His Pro Asp Val Asp Phe 
465                 470                 475                 480 


Ile Tyr Ala Cys Ser Thr Asp Val Ala Leu Gly Ala Val Asp Ala Leu 
                485                 490                 495     


Ala Glu Leu Gly Arg Glu Asp Ile Met Ile Asn Gly Trp Gly Gly Gly 
            500                 505                 510         


Ser Ala Glu Leu Asp Ala Ile Gln Lys Gly Asp Leu Asp Ile Thr Val 
        515                 520                 525             


Met Arg Met Asn Asp Asp Thr Gly Ile Ala Met Ala Glu Ala Ile Lys 
    530                 535                 540                 


Trp Asp Leu Glu Asp Lys Pro Val Pro Thr Val Tyr Ser Gly Asp Phe 
545                 550                 555                 560 


Glu Ile Val Thr Lys Ala Asp Ser Pro Glu Arg Ile Glu Ala Leu Lys 
                565                 570                 575     


Lys Arg Ala Phe Arg Tyr Ser Asp Asn Gly Gly Ala Ala Ala Met Ala 
            580                 585                 590         


Ser Leu Pro Ala Thr His Glu Leu His Ile Phe Gly Ser Ile Asn Gly 
        595                 600                 605             


Val Asp Phe Asp Met Val Gly Gln Gly Thr Gly Asn Pro Asn Asp Gly 
    610                 615                 620                 


Tyr Glu Glu Leu Asn Leu Lys Ser Thr Lys Gly Asp Leu Gln Phe Ser 
625                 630                 635                 640 


Pro Trp Ile Leu Val Pro His Ile Gly Tyr Gly Phe His Gln Tyr Leu 
                645                 650                 655     


Pro Tyr Pro Asp Gly Met Ser Pro Phe Gln Ala Ala Met Val Asp Gly 
            660                 665                 670         


Ser Gly Tyr Gln Val His Arg Thr Met Gln Phe Glu Asp Gly Ala Ser 
        675                 680                 685             


Leu Thr Val Asn Tyr Arg Tyr Thr Tyr Glu Gly Ser His Ile Lys Gly 
    690                 695                 700                 


Glu Ala Gln Val Lys Gly Thr Gly Phe Pro Ala Asp Gly Pro Val Met 
705                 710                 715                 720 


Thr Asn Ser Leu Thr Ala Ala Asp Trp Cys Arg Ser Lys Lys Thr Tyr 
                725                 730                 735     


Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys Trp Ser Tyr Thr Thr 
            740                 745                 750         


Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg Thr Thr Tyr Thr Phe 
        755                 760                 765             


Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn Gln Pro Met Tyr Val 
    770                 775                 780                 


Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr Glu Leu Asn Phe Lys 
785                 790                 795                 800 


Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly Met Asp Glu Leu Tyr 
                805                 810                 815     


Lys 
    


<210>  4
<211>  1043
<212>  DNA
<213>  Vibrio harveyi

<400>  4
atggtatcaa agggtgtgct taatggctat tggggctatc aggagtttct ggatgagttc       60

ccagaacagc gcaatcttac gaacgccctg tctgaagcgg ttcgggcgca gcctgtgcct      120

ctgtctaagc cgactcaacg tcctatcaag atttcggtcg tataccctgg gcagcaagtt      180

tccgattact gggtgcgtaa catcgcaagc tttgaaaaac gtctgtataa actgaacatt      240

aattaccaac tgaaccaagt gttcacccga cccaatgccg atattaaaca acagtcactc      300

agtctgatgg aggccctgaa atccaaatcg gactacctga tctttacctt ggatacgact      360

cgccaccgta aatttgtgga gcacgtactc gatagtacaa atacgaaact gattttacag      420

aatataacga cgccggtacg ggaatgggac aaacaccagc cgttcctgta tgtgggtttt      480

gaccatgctg aaggttcccg agaactggca accgaattcg gtaaattttt cccgaaacat      540

acgtattact ctgtgctcta tttctcggaa ggctacatca gcgatgtacg tggagatacc      600

tttatccatc aggtgaatcg ggataataac tttgagcttc agtccgctta ctacaccaag      660

gcgaccaagc aatccggtta tgatgctgcc aaggcgagct tggcaaagca ccctgacgtt      720

gactttattt atgcctgctc gacggacgta gctctgggtg cagtagatgc actggcagag      780

ctcgggagag aggatattat gatcaacggt tggggagggg ggtccgcgga attagacgcg      840

atacagaaag gtgatctgga tattaccgtc atgcgtatga acgatgacac gggtattgcc      900

atggcagaag cgattaaatg ggatctggaa gacaaaccag taccgaccgt atatagtggc      960

gactttgaaa tcgtaaccaa agcggattcc ccggaacgaa tcgaagcgct gaagaagcgt     1020

gcttttcgct attccgataa cgg                                             1043


<210>  5
<211>  348
<212>  PRT
<213>  Vibrio harveyi

<400>  5

Met Val Ser Lys Gly Val Leu Asn Gly Tyr Trp Gly Tyr Gln Glu Phe 
1               5                   10                  15      


Leu Asp Glu Phe Pro Glu Gln Arg Asn Leu Thr Asn Ala Leu Ser Glu 
            20                  25                  30          


Ala Val Arg Ala Gln Pro Val Pro Leu Ser Lys Pro Thr Gln Arg Pro 
        35                  40                  45              


Ile Lys Ile Ser Val Val Tyr Pro Gly Gln Gln Val Ser Asp Tyr Trp 
    50                  55                  60                  


Val Arg Asn Ile Ala Ser Phe Glu Lys Arg Leu Tyr Lys Leu Asn Ile 
65                  70                  75                  80  


Asn Tyr Gln Leu Asn Gln Val Phe Thr Arg Pro Asn Ala Asp Ile Lys 
                85                  90                  95      


Gln Gln Ser Leu Ser Leu Met Glu Ala Leu Lys Ser Lys Ser Asp Tyr 
            100                 105                 110         


Leu Ile Phe Thr Leu Asp Thr Thr Arg His Arg Lys Phe Val Glu His 
        115                 120                 125             


Val Leu Asp Ser Thr Asn Thr Lys Leu Ile Leu Gln Asn Ile Thr Thr 
    130                 135                 140                 


Pro Val Arg Glu Trp Asp Lys His Gln Pro Phe Leu Tyr Val Gly Phe 
145                 150                 155                 160 


Asp His Ala Glu Gly Ser Arg Glu Leu Ala Thr Glu Phe Gly Lys Phe 
                165                 170                 175     


Phe Pro Lys His Thr Tyr Tyr Ser Val Leu Tyr Phe Ser Glu Gly Tyr 
            180                 185                 190         


Ile Ser Asp Val Arg Gly Asp Thr Phe Ile His Gln Val Asn Arg Asp 
        195                 200                 205             


Asn Asn Phe Glu Leu Gln Ser Ala Tyr Tyr Thr Lys Ala Thr Lys Gln 
    210                 215                 220                 


Ser Gly Tyr Asp Ala Ala Lys Ala Ser Leu Ala Lys His Pro Asp Val 
225                 230                 235                 240 


Asp Phe Ile Tyr Ala Cys Ser Thr Asp Val Ala Leu Gly Ala Val Asp 
                245                 250                 255     


Ala Leu Ala Glu Leu Gly Arg Glu Asp Ile Met Ile Asn Gly Trp Gly 
            260                 265                 270         


Gly Gly Ser Ala Glu Leu Asp Ala Ile Gln Lys Gly Asp Leu Asp Ile 
        275                 280                 285             


Thr Val Met Arg Met Asn Asp Asp Thr Gly Ile Ala Met Ala Glu Ala 
    290                 295                 300                 


Ile Lys Trp Asp Leu Glu Asp Lys Pro Val Pro Thr Val Tyr Ser Gly 
305                 310                 315                 320 


Asp Phe Glu Ile Val Thr Lys Ala Asp Ser Pro Glu Arg Ile Glu Ala 
                325                 330                 335     


Leu Lys Lys Arg Ala Phe Arg Tyr Ser Asp Asn Gly 
            340                 345             


<210>  6
<211>  699
<212>  DNA
<213>  Branchiostoma lanceolatum

<400>  6
atggcaagcc ttccggctac ccatgagctt catattttcg gcagtattaa tggcgtagac       60

tttgacatgg tgggtcaggg aaccggaaat cccaatgatg gctatgaaga gttgaacctg      120

aaatctacca agggcgacct ccagttttca ccttggattt tggtgccaca catcggttat      180

ggatttcatc agtatttacc ttatccggat ggaatgagtc cgttccaggc ggccatggtt      240

gatggttccg gttatcaggt ccatcgcacg atgcagttcg aggacggggc ctctctgacg      300

gtgaattatc gttataccta tgaaggttca catataaaag gtgaagctca ggtaaaaggc      360

acgggttttc cggcggatgg cccggtgatg accaactccc tcacggcggc ggactggtgc      420

cggagcaaaa agacgtatcc gaatgataaa accatcatta gcacctttaa atggtcatac      480

acgactggca acggtaaacg ctacagatcc accgctcgca ccacatatac ttttgctaaa      540

cctatggccg caaactattt gaaaaaccag cctatgtacg ttttccgtaa aaccgaactg      600

aagcacagta aaactgaact gaatttcaaa gaatggcaaa aagcatttac ggatgttatg      660

ggtatggatg agctgtataa gcatcaccat catcatcat                             699


<210>  7
<211>  242
<212>  PRT
<213>  Branchiostoma lanceolatum

<400>  7

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr 
1               5                   10                  15      


His Glu Leu His Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met 
            20                  25                  30          


Val Gly Gln Gly Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn 
        35                  40                  45              


Leu Lys Ser Thr Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val 
    50                  55                  60                  


Pro His Ile Gly Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly 
65                  70                  75                  80  


Met Ser Pro Phe Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val 
                85                  90                  95      


His Arg Thr Met Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr 
            100                 105                 110         


Arg Tyr Thr Tyr Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys 
        115                 120                 125             


Gly Thr Gly Phe Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr 
    130                 135                 140                 


Ala Ala Asp Trp Cys Arg Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr 
145                 150                 155                 160 


Ile Ile Ser Thr Phe Lys Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg 
                165                 170                 175     


Tyr Arg Ser Thr Ala Arg Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala 
            180                 185                 190         


Ala Asn Tyr Leu Lys Asn Gln Pro Met Tyr Val Phe Arg Lys Thr Glu 
        195                 200                 205             


Leu Lys His Ser Lys Thr Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala 
    210                 215                 220                 


Phe Thr Asp Val Met Gly Met Asp Glu Leu Tyr Lys His His His His 
225                 230                 235                 240 


His His 
        


<210>  8
<211>  741
<212>  DNA
<213>  Aequorea victroria

<400>  8
atggtatcaa agggtgaaga acttttcacc ggtgttgtgc caattttggt cgagttagac       60

ggtgatgtga atggccacaa attctctgtc tccggcgaag gtgagggtga tgcaacctat      120

ggcaaactga cgctgaaatt tatttgcacc accgggaagc ttcccgttcc ttggcctact      180

ttggttacca ccttgtcttg gggtgtgcag tgcttcgcac gttatcctga ccacatgaaa      240

cagcacgatt tttttaaaag tgcaatgccg gaaggctatg tccaggaacg tactattttt      300

tttaaggatg atggcaacta taaaacccgt gccgaggtca aatttgaagg cgacaccttg      360

gttaaccgaa ttgaactgaa aggtattgat tttaaagaag atggtaatat tctgggtcac      420

aaattggagt ataactattt cagcgacaac gtttatatta ccgcagataa acaaaaaaac      480

ggtatcaaag ccaactttaa aattagacac aatatcgaag atggcggtgt ccaattagcc      540

gaccactatc agcagaacac gccgatcggt gatggtcctg tgctgctgcc ggataatcat      600

tacctttcaa cacagagcaa actgagcaaa gacccgaacg agaaacgtga ccacatggta      660

ctgctggaat ttgtgacagc tgcgggcatt acgcttggga tggacgagct atataaacat      720

caccatcatc atcattaata g                                                741


<210>  9
<211>  245
<212>  PRT
<213>  Aequorea victroria

<400>  9

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Ser Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Phe Ser Asp Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys His 
225                 230                 235                 240 


His His His His His 
                245 


<210>  10
<211>  7842
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid - pET-21a(+)-TqLPNGh

<400>  10
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt      480

tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta      540

tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat      600

gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt      660

ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg      720

agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga      780

agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg      840

tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt      900

tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg      960

cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg     1020

aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga     1080

tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc     1140

tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc     1200

ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc     1260

ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg     1320

cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac     1380

gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc     1440

actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt     1500

aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac     1560

caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa     1620

aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc     1680

accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt     1740

aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg     1800

ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc     1860

agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt     1920

accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga     1980

gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct     2040

tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg     2100

cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca     2160

cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa     2220

cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt     2280

ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga     2340

taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga     2400

gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg     2460

tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat     2520

cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct     2580

gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct     2640

gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct     2700

catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt     2760

tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg     2820

ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa     2880

tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc     2940

ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa     3000

aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta     3060

gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg     3120

tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag     3180

acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac     3240

cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca     3300

cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg     3360

gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc     3420

cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg     3480

gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca     3540

tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag     3600

atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt     3660

tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag     3720

gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc     3780

tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc     3840

cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct     3900

tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta     3960

atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg     4020

atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct     4080

tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga     4140

cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc     4200

aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg     4260

ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct     4320

tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt     4380

tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc     4440

gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc     4500

gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc     4560

gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact     4620

ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga     4680

taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc     4740

ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg     4800

atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag     4860

tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc     4920

gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat     4980

gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc     5040

aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat     5100

ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc     5160

cctctagaaa taattttgtt taactttaag aaggagatat acatatggta tcaaagggtg     5220

aagaactttt caccggtgtt gtgccaattt tggtcgagtt agacggtgat gtgaatggcc     5280

acaaattctc tgtctccggc gaaggtgagg gtgatgcaac ctatggcaaa ctgacgctga     5340

aatttatttg caccaccggg aagcttcccg ttccttggcc tactttggtt accaccttgt     5400

cttggggtgt gcagtgcttc gcacgttatc ctgaccacat gaaacagcac gattttttta     5460

aaagtgcaat gccggaaggc tatgtccagg aacgtactat tttttttaag gatgatggca     5520

actataaaac ccgtgccgag gtcaaatttg aaggcgacac cttggttaac cgaattgaac     5580

tgaaaggtat tgattttaaa gaagatggta atattctggg tcacaaattg gagtataact     5640

atttcagcga caacgtttat attaccgcag ataaacaaaa aaacggtatc aaagccaact     5700

ttaaaattag acacaatatc gaagatggcg gtgtccaatt agccgaccac tatcagcaga     5760

acacgccgat cggtgatggt cctgtgctgc tgccggataa tcattacctt tcaacacaga     5820

gcaaactgag caaagacccg aacgagaaac gtgaccacat ggtactgctg gaatttgtga     5880

cagctgcggg cattacgctt gggatggacg agctatataa aacggccggg ggtgtgctta     5940

atggctattg gggctatcag gagtttctgg atgagttccc agaacagcgc aatcttacga     6000

acgccctgtc tgaagcggtt cgggcgcagc ctgtgcctct gtctaagccg actcaacgtc     6060

ctatcaagat ttcggtcgta taccctgggc agcaagtttc cgattactgg gtgcgtaaca     6120

tcgcaagctt tgaaaaacgt ctgtataaac tgaacattaa ttaccaactg aaccaagtgt     6180

tcacccgacc caatgccgat attaaacaac agtcactcag tctgatggag gccctgaaat     6240

ccaaatcgga ctacctgatc tttaccttgg atacgactcg ccaccgtaaa tttgtggagc     6300

acgtactcga tagtacaaat acgaaactga ttttacagaa tataacgacg ccggtacggg     6360

aatgggacaa acaccagccg ttcctgtatg tgggttttga ccatgctgaa ggttcccgag     6420

aactggcaac cgaattcggt aaatttttcc cgaaacatac gtattactct gtgctctatt     6480

tctcggaagg ctacatcagc gatgtacgtg gagatacctt tatccatcag gtgaatcggg     6540

ataataactt tgagcttcag tccgcttact acaccaaggc gaccaagcaa tccggttatg     6600

atgctgccaa ggcgagcttg gcaaagcacc ctgacgttga ctttatttat gcctgctcga     6660

cggacgtagc tctgggtgca gtagatgcac tggcagagct cgggagagag gatattatga     6720

tcaacggttg gggagggggg tccgcggaat tagacgcgat acagaaaggt gatctggata     6780

ttaccgtcat gcgtatgaac gatgacacgg gtattgccat ggcagaagcg attaaatggg     6840

atctggaaga caaaccagta ccgaccgtat atagtggcga ctttgaaatc gtaaccaaag     6900

cggattcccc ggaacgaatc gaagcgctga agaagcgtgc ttttcgctat tccgataacg     6960

gtggggcggc tgccatggca agccttccgg ctacccatga gcttcatatt ttcggcagta     7020

ttaatggcgt agactttgac atggtgggtc agggaaccgg aaatcccaat gatggctatg     7080

aagagttgaa cctgaaatct accaagggcg acctccagtt ttcaccttgg attttggtgc     7140

cacacatcgg ttatggattt catcagtatt taccttatcc ggatggaatg agtccgttcc     7200

aggcggccat ggttgatggt tccggttatc aggtccatcg cacgatgcag ttcgaggacg     7260

gggcctctct gacggtgaat tatcgttata cctatgaagg ttcacatata aaaggtgaag     7320

ctcaggtaaa aggcacgggt tttccggcgg atggcccggt gatgaccaac tccctcacgg     7380

cggcggactg gtgccggagc aaaaagacgt atccgaatga taaaaccatc attagcacct     7440

ttaaatggtc atacacgact ggcaacggta aacgctacag atccaccgct cgcaccacat     7500

atacttttgc taaacctatg gccgcaaact atttgaaaaa ccagcctatg tacgttttcc     7560

gtaaaaccga actgaagcac agtaaaactg aactgaattt caaagaatgg caaaaagcat     7620

ttacggatgt tatgggtatg gatgagctgt ataagcatca ccatcatcat cattaatagc     7680

tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg     7740

agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg     7800

tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at                        7842


<210>  11
<211>  7860
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid - pET21-hTqLPNG

<400>  11
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt      480

tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta      540

tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat      600

gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt      660

ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg      720

agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga      780

agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg      840

tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt      900

tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg      960

cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg     1020

aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga     1080

tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc     1140

tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc     1200

ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc     1260

ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg     1320

cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac     1380

gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc     1440

actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt     1500

aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac     1560

caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa     1620

aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc     1680

accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt     1740

aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg     1800

ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc     1860

agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt     1920

accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga     1980

gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct     2040

tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg     2100

cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca     2160

cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa     2220

cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt     2280

ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga     2340

taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga     2400

gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg     2460

tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat     2520

cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct     2580

gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct     2640

gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct     2700

catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt     2760

tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg     2820

ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa     2880

tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc     2940

ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa     3000

aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta     3060

gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg     3120

tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag     3180

acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac     3240

cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca     3300

cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg     3360

gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc     3420

cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg     3480

gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca     3540

tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag     3600

atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt     3660

tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag     3720

gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc     3780

tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc     3840

cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct     3900

tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta     3960

atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg     4020

atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct     4080

tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga     4140

cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc     4200

aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg     4260

ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct     4320

tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt     4380

tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc     4440

gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc     4500

gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc     4560

gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact     4620

ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga     4680

taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc     4740

ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg     4800

atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag     4860

tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc     4920

gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat     4980

gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc     5040

aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat     5100

ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc     5160

cctctagaaa taattttgtt taactttaag aaggagatat acatatgggc agcagccatc     5220

atcatcatca tcacggaagc agtggccgta aaggcgagga gctgttcaca ggcgtcgtgc     5280

cgatcctggt agaactcgat ggggatgtca atggacataa gtttagcgtc tcgggagaag     5340

gggagggtga cgccacgtat gggaagctta cgctgaaatt tatttgcacc acgggcaaat     5400

taccggtccc gtggcctacc cttgtaacca ctctctcttg gggagtgcag tgcttcgcga     5460

ggtacccaga tcacatgaaa caacatgatt ttttcaaatc ggcgatgccg gaaggctatg     5520

tgcaagagcg cacaatcttt tttaaagatg acggtaatta caagacccga gccgaagtaa     5580

aatttgaggg agatacactg gtgaaccgta ttgaactgaa aggtattgac tttaaagagg     5640

acggcaatat cctgggccat aagctggaat acaattattt ttctgataac gtatatatta     5700

cggccgacaa acagaagaac ggtatcaaag cgaattttaa aattcgccac aatatcgaag     5760

acgggggtgt tcagttggcc gatcattatc aacagaacac tccgattggg gatggccccg     5820

tactgctgcc agataatcat tacctctcta ctcagtcaaa gctgagcaaa gatccaaacg     5880

agaaacgtga tcacatggtt ttactggagt ttgtcacggc cgccggtatt accctgggaa     5940

tggatgaact gtataagacc gcagggggcg tgctgaacgg ctattggggc tatcaggaat     6000

tcctggacga atttccggaa cagcgaaatt taaccaacgc actgagcgaa gcagtacgcg     6060

cccaaccggt gcctctgtct aaacccaccc aacgcccgat taagatttct gttgtctacc     6120

cggggcagca ggtgtctgat tactgggtcc gcaacattgc ctcctttgag aagaggctgt     6180

ataagcttaa tatcaattac cagctgaacc aagtgttcac tcgccctaat gcggatatca     6240

aacagcaatc gctttcgctg atggaagctc tgaaaagtaa atctgactat ctcatcttta     6300

ccctggacac gacccgtcac agaaagttcg tcgaacacgt ccttgattca acaaacacaa     6360

aactaattct tcagaacatc actacgccag tgcgtgaatg ggataaacat cagccgtttc     6420

tttatgtggg ctttgaccat gccgaaggca gccgtgagtt agcaacggag tttgggaagt     6480

ttttcccgaa acatacatac tactctgttc tgtattttag cgagggatat atctcggacg     6540

tgcgcggtga tacctttatt catcaggtaa accgcgataa caactttgaa ctgcaaagcg     6600

cgtattatac gaaagctacc aaacaaagcg gctatgacgc agcgaaagcg tccctggcga     6660

agcatccgga tgtcgatttt atttacgcat gcagtaccga cgtagcatta ggggcggttg     6720

acgcactggc tgagttaggt cgcgaggata ttatgattaa tggttggggt gggggtagtg     6780

ccgaattgga cgctattcag aaaggtgacc tggatattac agtaatgcgt atgaacgatg     6840

atactggcat tgccatggca gaggcaatca aatgggatct ggaagataaa cctgttccca     6900

cggtatattc cggtgatttt gaaattgtca ctaaagcaga cagtccggaa cgtatagaag     6960

cactcaaaaa gcgtgctttc cgttactcag ataatggggg cgctgccgcc atggcctctc     7020

tgccagccac ccatgaactg cacatttttg gttcgattaa cggtgttgac ttcgacatgg     7080

tagggcaggg taccggcaat ccgaatgacg gctatgaaga attgaacctg aaatcaacga     7140

aaggcgattt gcagttttct ccgtggatcc tggtgccgca cattggctac ggatttcacc     7200

agtatcttcc gtaccccgat gggatgtcac catttcaggc ggctatggtg gatggttccg     7260

gctatcaggt acatcgcacg atgcagtttg aagacggcgc gagcctgacg gttaattatc     7320

gctacacgta tgagggtagc cacatcaaag gcgaagcgca agtaaaaggt accggttttc     7380

cggccgatgg accggttatg acgaacagtc taacggcagc cgactggtgt cggtcgaaga     7440

aaacgtatcc gaatgataag accattattt cgacgttcaa gtggtcctat acgaccggaa     7500

acggtaagcg ctatcggtct accgcacgca cgacatacac tttcgctaaa ccgatggctg     7560

ccaactacct taaaaatcag ccgatgtatg tattccgaaa aacagaatta aaacattcta     7620

aaaccgaact taattttaaa gaatggcaaa aagcctttac agatgtgatg ggcatggacg     7680

aattgtacaa ataatagctc gagcaccacc accaccacca ctgagatccg gctgctaaca     7740

aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc     7800

ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat     7860


