                         SEQUENCE LISTING

<110>  The University of Chicago
 
<120>  Cancer Treatment with 237 CAR-T- Cell-Based Therapeutics 
       recognizing the Tn Epitope

<130>  27373/52963A

<150>  US 62/729,951
<151>  2018-09-11

<160>  80    

<170>  PatentIn version 3.5

<210>  1
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  237 scFv Variable Heavy Region

<400>  1

Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Met Lys Ile Phe Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ala 
            20                  25                  30          


Trp Met Asp Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Glu Ile Arg Asn Lys Ala Asn Asn His Glu Thr Tyr Tyr Ala Glu 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Thr Arg Asp Asp Ser Lys Ser Arg 
65                  70                  75                  80  


Met Ser Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Ile Tyr 
                85                  90                  95      


Tyr Cys Ser Gly Gly Lys Val Arg Asn Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Val Thr Val Ser Ser 
        115             


<210>  2
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  237 scFv Variable Light Region

<400>  2

Asp Ile Gln Leu Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Ser Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  3
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  CDRL3 sequence

<400>  3

Thr Thr Trp Ala Pro 
1               5   


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  CDRL3 sequence

<400>  4

Ser Thr Trp Ala Pro 
1               5   


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  CDRL3 sequence

<400>  5

Ser Thr Trp Ser Pro 
1               5   


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  CDRL3 sequence

<400>  6

Ser Thr Trp Gly Pro 
1               5   


<210>  7
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  CDRL3 sequence

<400>  7

Ser Thr Trp Gln Pro 
1               5   


<210>  8
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  CDRL3 sequence

<400>  8

Ser Thr Trp Glu Pro 
1               5   


<210>  9
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  CDRL3 sequence

<400>  9

Ser Val Trp Glu Pro 
1               5   


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  4 Ala substitutions for PPLE of the wild-type PDPN binding site 
       for the 237 antibody

<220>
<221>  MISC_FEATURE
<223>  peptide tested for 237 CAR-T cell binding

<400>  10

Gly Thr Lys Ala Ala Ala Ala 
1               5           


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  wild-type PDPN binding site for the 237 antibody

<400>  11

Gly Thr Lys Pro Pro Leu Glu 
1               5           


<210>  12
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  Tn-OTS8 peptide

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  GalNAc derivatization of T13

<400>  12

Lys Ala Pro Leu Val Pro Thr Gln Arg Glu Arg Gly Thr Lys Pro Pro 
1               5                   10                  15      


Leu Glu Glu Leu Ser Thr Ser Ala Thr Ser Asp His Asp His 
            20                  25                  30  


<210>  13
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  tetrameric or monomeric Tn-OTS8 peptide

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  GalNAc

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Biotin

<400>  13

Glu Arg Gly Thr Lys Pro Pro Leu Glu Glu Leu Ser Gly Lys 
1               5                   10                  


<210>  14
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  minimal epitope -epitope within the CA125 epitope of MUC16.

<400>  14

Phe Asn Thr Thr Glu Arg 
1               5       


<210>  15
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  epitope of MUC4

<400>  15

Ser Thr Gly Asp Thr Thr Pro Leu Pro Val Thr Asp Thr Ser Ser Val 
1               5                   10                  15      


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  glycosylation site of MUC4

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  GalNAc derivatization of T4

<400>  16

Gly His Ala Thr Pro Leu Pro Val Thr Asp 
1               5                   10  


<210>  17
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  5E5 binding site on MUC1

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  GalNAc derivatization of T2

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  GalNAc derivatization of S13

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  GalNAc derivatization of T14

<400>  17

Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro 
1               5                   10                  15      


Pro Ala His Gly 
            20  


<210>  18
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  peptide tested for 237 CAR-T cell binding

<400>  18

Gly Thr Lys Pro Pro Leu Glu Glu 
1               5               


<210>  19
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  237-wt scFv

<220>
<221>  MISC_FEATURE
<223>  (1)-(111) 237 scFv Variable Light Region

<220>
<221>  MISC_FEATURE
<223>  (112)-(126) Linker

<220>
<221>  MISC_FEATURE
<223>  (127)-(244) scFv Variable Heavy Region

<400>  19

Asp Ile Gln Leu Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Ser Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val 
        115                 120                 125             


Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Met 
    130                 135                 140                 


Lys Ile Phe Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ala Trp Met 
145                 150                 155                 160 


Asp Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Glu 
                165                 170                 175     


Ile Arg Asn Lys Ala Asn Asn His Glu Thr Tyr Tyr Ala Glu Ser Val 
            180                 185                 190         


Lys Gly Arg Phe Thr Ile Thr Arg Asp Asp Ser Lys Ser Arg Met Ser 
        195                 200                 205             


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Ile Tyr Tyr Cys 
    210                 215                 220                 


Ser Gly Gly Lys Val Arg Asn Ala Tyr Trp Gly Gln Gly Thr Thr Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  20
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (98)..(99)
<223>  237-WE scFv variant

<400>  20

Asp Ile Gln Leu Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Ser Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr Trp Glu Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val 
        115                 120                 125             


Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Met 
    130                 135                 140                 


Lys Ile Phe Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ala Trp Met 
145                 150                 155                 160 


Asp Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Glu 
                165                 170                 175     


Ile Arg Asn Lys Ala Asn Asn His Glu Thr Tyr Tyr Ala Glu Ser Val 
            180                 185                 190         


Lys Gly Arg Phe Thr Ile Thr Arg Asp Asp Ser Lys Ser Arg Met Ser 
        195                 200                 205             


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Ile Tyr Tyr Cys 
    210                 215                 220                 


Ser Gly Gly Lys Val Arg Asn Ala Tyr Trp Gly Gln Gly Thr Thr Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  21
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (97)..(99)
<223>  237-LGQ scFv variant

<400>  21

Asp Ile Gln Leu Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Ser Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Leu Gly Gln Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val 
        115                 120                 125             


Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Met 
    130                 135                 140                 


Lys Ile Phe Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ala Trp Met 
145                 150                 155                 160 


Asp Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Glu 
                165                 170                 175     


Ile Arg Asn Lys Ala Asn Asn His Glu Thr Tyr Tyr Ala Glu Ser Val 
            180                 185                 190         


Lys Gly Arg Phe Thr Ile Thr Arg Asp Asp Ser Lys Ser Arg Met Ser 
        195                 200                 205             


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Ile Tyr Tyr Cys 
    210                 215                 220                 


Ser Gly Gly Lys Val Arg Asn Ala Tyr Trp Gly Gln Gly Thr Thr Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  22
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (96)..(99)
<223>  237-TNGK scFv variant

<400>  22

Asp Ile Gln Leu Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Ser Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Thr 
                85                  90                  95      


Asn Gly Lys Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val 
        115                 120                 125             


Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Met 
    130                 135                 140                 


Lys Ile Phe Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ala Trp Met 
145                 150                 155                 160 


Asp Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Glu 
                165                 170                 175     


Ile Arg Asn Lys Ala Asn Asn His Glu Thr Tyr Tyr Ala Glu Ser Val 
            180                 185                 190         


Lys Gly Arg Phe Thr Ile Thr Arg Asp Asp Ser Lys Ser Arg Met Ser 
        195                 200                 205             


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Ile Tyr Tyr Cys 
    210                 215                 220                 


Ser Gly Gly Lys Val Arg Asn Ala Tyr Trp Gly Gln Gly Thr Thr Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  23
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (97)..(99)
<223>  237-LGQ scFv variant

<400>  23

Asp Ile Gln Leu Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Ser Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Leu Gly Gln Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val 
        115                 120                 125             


Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Met 
    130                 135                 140                 


Lys Ile Phe Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ala Trp Met 
145                 150                 155                 160 


Asp Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Glu 
                165                 170                 175     


Ile Arg Asn Lys Ala Asn Asn His Glu Thr Tyr Tyr Ala Glu Ser Val 
            180                 185                 190         


Lys Gly Arg Phe Thr Ile Thr Arg Asp Asp Ser Lys Ser Arg Met Ser 
        195                 200                 205             


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Ile Tyr Tyr Cys 
    210                 215                 220                 


Ser Gly Gly Lys Val Arg Asn Ala Tyr Trp Gly Gln Gly Thr Thr Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  24
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (96)..(99)
<223>  237-TNGK scFv variant

<400>  24

Asp Ile Gln Leu Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Ser Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Thr 
                85                  90                  95      


Asn Gly Lys Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val 
        115                 120                 125             


Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Met 
    130                 135                 140                 


Lys Ile Phe Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ala Trp Met 
145                 150                 155                 160 


Asp Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Glu 
                165                 170                 175     


Ile Arg Asn Lys Ala Asn Asn His Glu Thr Tyr Tyr Ala Glu Ser Val 
            180                 185                 190         


Lys Gly Arg Phe Thr Ile Thr Arg Asp Asp Ser Lys Ser Arg Met Ser 
        195                 200                 205             


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Ile Tyr Tyr Cys 
    210                 215                 220                 


Ser Gly Gly Lys Val Arg Asn Ala Tyr Trp Gly Gln Gly Thr Thr Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  25
<211>  6996
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  237 CAR Sequence

<400>  25
tcaaggttag gaacagagag acaggagaat atgggccaaa caggatatct gtggtaagca       60

gttcctgccc cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga      120

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc      180

ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc      240

tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc      300

gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc      360

ctccgattga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca      420

tccgaatcgt ggactcgctg atccttggga gggtctcctc agattgattg actgcccacc      480

tcgggggtct ttcatttgga ggttccaccg agatttggag acccctgccc agggaccacc      540

gacccccccg ccgggaggta agctggccag cggtcgtttc gtgtctgtct ctgtctttgg      600

gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg tactagttgg ctaactagat      660

ctgtatctgg cggtcccgcg gaagaactga cgagttcgta ttcccggccg cagcccctgg      720

gagacgtccc agcggcctcg ggggcccgtt ttgtggccca ttctgtatca gttaacctac      780

ccgagtcgga ctttttggag ctccgccact gtccgagggg tacgtggctt tgttggggga      840

cgagagacag agacacttcc cgcccccgtc tgaatttttg ctttcggttt tacgccgaaa      900

ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt      960

tctgtatttg tctgaaaatt agctcgacaa agttaagtaa tagtccctct ctccaagctc     1020

acttacaggc ggccgccacc atggggctgg tctgcatcat cctgtttctg gtggccacag     1080

ccaccggcgt gcacagcgat atccagctga cacagagccc cctgagcctg cctgtgtctc     1140

tgggcgatca ggccagcatc agctgcagat ccagccagag cctggtgcac agcaacggca     1200

acacctacct gcactggtat ctgcagaagc ccggccagag ccccaagctg ctgatctaca     1260

aggtgtccaa cagattcagc ggcgtgcccg acagattctc cggcagcggc tctggcaccg     1320

acttcaccct gaagatcagc tccgtggaag ccgaggacct gggcgtgtac ttctgcagcc     1380

agtccaccca cgtgcccaca ttcggcggag gcaccaagct ggaaatcaag ggcggaggcg     1440

gatctggcgg cggaggatct gggggaggcg gctctcaggt gcagctgcag cagtctggcg     1500

gagggctggt gcagcctggc ggcagcatga agatcttttg cgccgcctcc ggcttcacct     1560

tcagcgacgc ttggatggac tgggtgcgac agagccctga gaagggcctg gaatgggtgg     1620

ccgagatcag aaacaaggcc aacaaccacg agacttacta cgccgagagc gtgaagggca     1680

gattcaccat caccagggac gacagcaaga gcagaatgag cctgcagatg aacagcctga     1740

gggccgagga caccggcatc tactactgca gcggcggcaa agtgcggaac gcctactggg     1800

gccagggcac cacagtgacc gtgtccagcc tcgagaaagt gaacagcacc accaccaagc     1860

ccgtgctgag aacccctagc cctgtgcacc ctaccggcac atctcagcct cagaggcccg     1920

aggactgcag acctagaggc tccgtgaagg gaaccggcct ggacttcgcc tgtgacttct     1980

gggcactggt ggtggtggcc ggcgtgctgt tttgttacgg cctgctcgtg accgtggccc     2040

tgtgcgtgat ctggactagt aaatggatcc ggaagaagtt cccccacatc ttcaagcagc     2100

ccttcaagaa aaccaccggc gctgcccagg aagaggacgc ctgcagctgt aggtgccctc     2160

aggaagaaga aggcggaggg ggcggatacg agctgagagc caagttcagc agaagcgccg     2220

agacagccgc caacctgcag gaccctaacc agctgtacaa cgagctgaac ctgggcagac     2280

gggaagagta cgacgtgctg gaaaagaaga gagccaggga ccccgagatg ggcggcaagc     2340

agcagagaag aagaaacccc caggaaggcg tgtacaacgc cctgcagaaa gacaagatgg     2400

ccgaggccta cagcgagatc ggcaccaagg gcgaaaggcg gagaggcaag ggacacgacg     2460

gactgtacca gggcctgtcc accgccacca aggacacata cgatgccctg cacatgcaga     2520

cactcgcccc cagatgatga gaattcgagc atcttaccgc catttattcc catatttgtt     2580

ctgtttttct tgatttgggt atacatttaa atgttaataa aacaaaatgg tggggcaatc     2640

atttacattt tatgggatat gtaattacta gttcaggtgt attgccacaa gacaaacatg     2700

ttaagaaact ttcccgttat ttacgctctg ttcctgttaa tcaacctctg gattacaaaa     2760

tttgtgaaag attgactgat attcttaact atgttgctcc ttttacgctg tgtggatatg     2820

ctgctttaat gcctctgtat catgctattg cttcccgtac ggctttcgtt ttctcctcct     2880

tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc cgtcaacgtg     2940

gcgtggtgtg ctctgtgttt gctgacgcaa cccccactgg ctggggcatt gccaccacct     3000

gtcaactcct ttctgggact ttcgctttcc ccctcccgat cgccacggca gaactcatcg     3060

ccgcctgcct tgcccgctgc tggacagggg ctaggttgct gggcactgat aattccgtgg     3120

tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc aactggatcc     3180

tgcgcgggac gtccttctgc tacgtccctt cggctctcaa tccagcggac ctcccttccc     3240

gaggccttct gccggttctg cggcctctcc cgcgtcttcg ctttcggcct ccgacgagtc     3300

ggatctccct ttgggccgcc tccccgcctg tttcgcctcg gcgtccggtc cgtgttgctt     3360

ggtcgtcacc tgtgcagaat tgcgaaccat ggattccacc gtgaactttg tctcctggca     3420

tgcaaatcgt caacttggca tgccaagaat taattcggat ccaagcttag gcctgctcgc     3480

tttcttgctg tcccatttct attaaaggtt cctttgttcc ctaagtccaa ctactaaact     3540

gggggatatt atgaagggcc ttgagcatct ggattctgcc tagcgctaag cttcctaaca     3600

cgagccatag atagaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga     3660

ccccacctgt aggtttggca agctagctta agtaagccat tttgcaaggc atggaaaaat     3720

acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca ggagaatatg     3780

ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagttg     3840

gaacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag     3900

ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc     3960

agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca     4020

atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc     4080

cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg ggtacccgta     4140

ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg actcgctgat ccttgggagg     4200

gtctcctcag attgattgac tgcccacctc gggggtcttt cattctcgag agctttggcg     4260

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac     4320

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca     4380

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat     4440

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc     4500

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca     4560

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca     4620

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg     4680

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg     4740

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt     4800

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt     4860

tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc     4920

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt     4980

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt     5040

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc     5100

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa     5160

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt     5220

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct     5280

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta     5340

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa     5400

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc     5460

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact     5520

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc     5580

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt     5640

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta     5700

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgctgg catcgtggtg     5760

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt     5820

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc     5880

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt     5940

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc     6000

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc     6060

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa     6120

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac     6180

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa     6240

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt     6300

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa     6360

tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct     6420

gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg     6480

ccctttcgtc ttcaagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca     6540

tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc     6600

gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta     6660

gcgatagtta ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg     6720

aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc     6780

tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga     6840

aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac     6900

gttgtaaaac gacggccagt gaattagtac tctagcttaa gtaagccatt ttgcaaggca     6960

tggaaaaata cataactgag aatagagaag ttcaga                               6996


<210>  26
<211>  6996
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide


<220>
<221>  misc_feature
<223>  plasmid pMP71-237-CD28tm-41BB-CD3z

<220>
<221>  misc_feature
<222>  (1098)..(1835)
<223>  237 scFV

<220>
<221>  misc_feature
<222>  (1836)..(1976)
<223>  CD8 linker

<220>
<221>  misc_feature
<222>  (1977)..(2060)
<223>  CD28 extracellular transmembrane region

<220>
<221>  misc_feature
<222>  (2061)..(2534)
<223>  4-1BB

<400>  26
tcaaggttag gaacagagag acaggagaat atgggccaaa caggatatct gtggtaagca       60

gttcctgccc cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga      120

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc      180

ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc      240

tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc      300

gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc      360

ctccgattga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca      420

tccgaatcgt ggactcgctg atccttggga gggtctcctc agattgattg actgcccacc      480

tcgggggtct ttcatttgga ggttccaccg agatttggag acccctgccc agggaccacc      540

gacccccccg ccgggaggta agctggccag cggtcgtttc gtgtctgtct ctgtctttgg      600

gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg tactagttgg ctaactagat      660

ctgtatctgg cggtcccgcg gaagaactga cgagttcgta ttcccggccg cagcccctgg      720

gagacgtccc agcggcctcg ggggcccgtt ttgtggccca ttctgtatca gttaacctac      780

ccgagtcgga ctttttggag ctccgccact gtccgagggg tacgtggctt tgttggggga      840

cgagagacag agacacttcc cgcccccgtc tgaatttttg ctttcggttt tacgccgaaa      900

ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt      960

tctgtatttg tctgaaaatt agctcgacaa agttaagtaa tagtccctct ctccaagctc     1020

acttacaggc ggccgccacc atggggctgg tctgcatcat cctgtttctg gtggccacag     1080

ccaccggcgt gcacagcgat atccagctga cacagagccc cctgagcctg cctgtgtctc     1140

tgggcgatca ggccagcatc agctgcagat ccagccagag cctggtgcac agcaacggca     1200

acacctacct gcactggtat ctgcagaagc ccggccagag ccccaagctg ctgatctaca     1260

aggtgtccaa cagattcagc ggcgtgcccg acagattctc cggcagcggc tctggcaccg     1320

acttcaccct gaagatcagc tccgtggaag ccgaggacct gggcgtgtac ttctgcagcc     1380

agtccaccca cgtgcccaca ttcggcggag gcaccaagct ggaaatcaag ggcggaggcg     1440

gatctggcgg cggaggatct gggggaggcg gctctcaggt gcagctgcag cagtctggcg     1500

gagggctggt gcagcctggc ggcagcatga agatcttttg cgccgcctcc ggcttcacct     1560

tcagcgacgc ttggatggac tgggtgcgac agagccctga gaagggcctg gaatgggtgg     1620

ccgagatcag aaacaaggcc aacaaccacg agacttacta cgccgagagc gtgaagggca     1680

gattcaccat caccagggac gacagcaaga gcagaatgag cctgcagatg aacagcctga     1740

gggccgagga caccggcatc tactactgca gcggcggcaa agtgcggaac gcctactggg     1800

gccagggcac cacagtgacc gtgtccagcc tcgagaaagt gaacagcacc accaccaagc     1860

ccgtgctgag aacccctagc cctgtgcacc ctaccggcac atctcagcct cagaggcccg     1920

aggactgcag acctagaggc tccgtgaagg gaaccggcct ggacttcgcc tgtgacttct     1980

gggcactggt ggtggtggcc ggcgtgctgt tttgttacgg cctgctcgtg accgtggccc     2040

tgtgcgtgat ctggactagt aaatggatcc ggaagaagtt cccccacatc ttcaagcagc     2100

ccttcaagaa aaccaccggc gctgcccagg aagaggacgc ctgcagctgt aggtgccctc     2160

aggaagaaga aggcggaggg ggcggatacg agctgagagc caagttcagc agaagcgccg     2220

agacagccgc caacctgcag gaccctaacc agctgtacaa cgagctgaac ctgggcagac     2280

gggaagagta cgacgtgctg gaaaagaaga gagccaggga ccccgagatg ggcggcaagc     2340

agcagagaag aagaaacccc caggaaggcg tgtacaacgc cctgcagaaa gacaagatgg     2400

ccgaggccta cagcgagatc ggcaccaagg gcgaaaggcg gagaggcaag ggacacgacg     2460

gactgtacca gggcctgtcc accgccacca aggacacata cgatgccctg cacatgcaga     2520

cactcgcccc cagatgatga gaattcgagc atcttaccgc catttattcc catatttgtt     2580

ctgtttttct tgatttgggt atacatttaa atgttaataa aacaaaatgg tggggcaatc     2640

atttacattt tatgggatat gtaattacta gttcaggtgt attgccacaa gacaaacatg     2700

ttaagaaact ttcccgttat ttacgctctg ttcctgttaa tcaacctctg gattacaaaa     2760

tttgtgaaag attgactgat attcttaact atgttgctcc ttttacgctg tgtggatatg     2820

ctgctttaat gcctctgtat catgctattg cttcccgtac ggctttcgtt ttctcctcct     2880

tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc cgtcaacgtg     2940

gcgtggtgtg ctctgtgttt gctgacgcaa cccccactgg ctggggcatt gccaccacct     3000

gtcaactcct ttctgggact ttcgctttcc ccctcccgat cgccacggca gaactcatcg     3060

ccgcctgcct tgcccgctgc tggacagggg ctaggttgct gggcactgat aattccgtgg     3120

tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc aactggatcc     3180

tgcgcgggac gtccttctgc tacgtccctt cggctctcaa tccagcggac ctcccttccc     3240

gaggccttct gccggttctg cggcctctcc cgcgtcttcg ctttcggcct ccgacgagtc     3300

ggatctccct ttgggccgcc tccccgcctg tttcgcctcg gcgtccggtc cgtgttgctt     3360

ggtcgtcacc tgtgcagaat tgcgaaccat ggattccacc gtgaactttg tctcctggca     3420

tgcaaatcgt caacttggca tgccaagaat taattcggat ccaagcttag gcctgctcgc     3480

tttcttgctg tcccatttct attaaaggtt cctttgttcc ctaagtccaa ctactaaact     3540

gggggatatt atgaagggcc ttgagcatct ggattctgcc tagcgctaag cttcctaaca     3600

cgagccatag atagaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga     3660

ccccacctgt aggtttggca agctagctta agtaagccat tttgcaaggc atggaaaaat     3720

acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca ggagaatatg     3780

ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagttg     3840

gaacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag     3900

ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc     3960

agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca     4020

atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc     4080

cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg ggtacccgta     4140

ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg actcgctgat ccttgggagg     4200

gtctcctcag attgattgac tgcccacctc gggggtcttt cattctcgag agctttggcg     4260

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac     4320

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca     4380

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat     4440

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc     4500

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca     4560

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca     4620

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg     4680

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg     4740

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt     4800

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt     4860

tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc     4920

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt     4980

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt     5040

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc     5100

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa     5160

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt     5220

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct     5280

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta     5340

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa     5400

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc     5460

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact     5520

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc     5580

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt     5640

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta     5700

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgctgg catcgtggtg     5760

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt     5820

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc     5880

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt     5940

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc     6000

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc     6060

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa     6120

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac     6180

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa     6240

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt     6300

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa     6360

tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct     6420

gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg     6480

ccctttcgtc ttcaagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca     6540

tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc     6600

gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta     6660

gcgatagtta ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg     6720

aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc     6780

tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga     6840

aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac     6900

gttgtaaaac gacggccagt gaattagtac tctagcttaa gtaagccatt ttgcaaggca     6960

tggaaaaata cataactgag aatagagaag ttcaga                               6996


<210>  27
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (98)..(98)
<223>  237 wt- H98W variant

<400>  27

Asp Ile Gln Leu Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Ser Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr Trp Val Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val 
        115                 120                 125             


Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Met 
    130                 135                 140                 


Lys Ile Phe Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ala Trp Met 
145                 150                 155                 160 


Asp Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Glu 
                165                 170                 175     


Ile Arg Asn Lys Ala Asn Asn His Glu Thr Tyr Tyr Ala Glu Ser Val 
            180                 185                 190         


Lys Gly Arg Phe Thr Ile Thr Arg Asp Asp Ser Lys Ser Arg Met Ser 
        195                 200                 205             


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Ile Tyr Tyr Cys 
    210                 215                 220                 


Ser Gly Gly Lys Val Arg Asn Ala Tyr Trp Gly Gln Gly Thr Thr Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  28
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (99)..(99)
<223>  237 wt- V99E variant

<400>  28

Asp Ile Gln Leu Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Ser Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Gln Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val 
        115                 120                 125             


Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Met 
    130                 135                 140                 


Lys Ile Phe Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ala Trp Met 
145                 150                 155                 160 


Asp Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Glu 
                165                 170                 175     


Ile Arg Asn Lys Ala Asn Asn His Glu Thr Tyr Tyr Ala Glu Ser Val 
            180                 185                 190         


Lys Gly Arg Phe Thr Ile Thr Arg Asp Asp Ser Lys Ser Arg Met Ser 
        195                 200                 205             


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Gly Ile Tyr Tyr Cys 
    210                 215                 220                 


Ser Gly Gly Lys Val Arg Asn Ala Tyr Trp Gly Gln Gly Thr Thr Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  29
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  5E5 Variable Light Region

<400>  29

Glu Leu Val Met Thr Gln Ser Pro Ser Ser Leu Thr Val Thr Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ile Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asp Gln Lys Asn Tyr Leu Thr Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Phe Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp Tyr Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105                 110         


Lys 
    


<210>  30
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<223>  5E5 Variable Heavy Region

<400>  30

Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Ala Ile His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly His Phe Ser Pro Gly Asn Thr Asp Ile Lys Tyr Asn Asp Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Lys Thr Ser Thr Phe Phe Phe Asp Tyr Trp Gly Gln Thr Thr Leu Thr 
            100                 105                 110         


Val Ser Ser Gly Ser 
        115         


<210>  31
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  MISC_FEATURE
<223>  wild-type PDPN binding site for the 237 antibody

<400>  31

Gly Thr Lys Pro Pro Leu Glu Glu 
1               5               


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  32
gatattgtga ccccaggtac                                                   20


<210>  33
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  33
caccgggaca cattaggatt gg                                                22


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  34
cggccacgga accagcttca                                                   20


<210>  35
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Tn modified

<400>  35

Gly Thr Lys Pro Pro Leu Glu Glu 
1               5               


<210>  36
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  36

Ala Pro Leu Val Pro Thr Gln Arg Glu Arg Gly 
1               5                   10      


<210>  37
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  37

Ser Thr Ala Pro Pro Ala His Gly 
1               5               


<210>  38
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  38

Glu Thr Pro His Ala Thr Ser His 
1               5               


<210>  39
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  39

Ser Thr Pro Pro Ser Val Thr Ser 
1               5               


<210>  40
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  40

Ser Thr Gln Pro Thr Ser Thr Val 
1               5               


<210>  41
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  41

Thr Thr Ala Pro Pro Ala Pro Pro 
1               5               


<210>  42
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  42

Ser Thr Lys Ala Glu His Leu Thr 
1               5               


<210>  43
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  43

Thr Thr Ser Ile Thr Ser Asp Pro 
1               5               


<210>  44
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  44

Thr Thr Ala Val Thr Pro Asn Gly 
1               5               


<210>  45
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  45

Gly Thr Glu Ser Pro Val Arg Glu 
1               5               


<210>  46
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  46

His Ser Asn Gly Asn Thr Tyr 
1               5           


<210>  47
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  47

Xaa Xaa Xaa Xaa Asn Thr Tyr 
1               5           


<210>  48
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  misc_feature
<222>  (4)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  48

His Ser Asn Xaa Xaa Xaa Xaa 
1               5           


<210>  49
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  49

Ser Thr His Val 
1               


<210>  50
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  50

Xaa Xaa Xaa Xaa 
1               


<210>  51
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  51

Glu Ile Arg Asn Lys Ala Asn Asn His Glu 
1               5                   10  


<210>  52
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  52

Xaa Xaa Xaa Xaa Lys Ala Asn Asn His Glu 
1               5                   10  


<210>  53
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  misc_feature
<222>  (4)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  53

Glu Ile Arg Xaa Xaa Xaa Xaa Asn His Glu 
1               5                   10  


<210>  54
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  misc_feature
<222>  (7)..(10)
<223>  Xaa can be any naturally occurring amino acid

<400>  54

Glu Ile Arg Asn Lys Ala Xaa Xaa Xaa Xaa 
1               5                   10  


<210>  55
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  55

Glu Arg Gly Thr Lys Pro Pro Leu Glu Glu Leu Ser 
1               5                   10          


<210>  56
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  56

Ala Thr Lys Pro Pro Leu Glu Glu 
1               5               


<210>  57
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  57

Gly Thr Ala Pro Pro Leu Glu Glu 
1               5               


<210>  58
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  58

Gly Thr Lys Ala Pro Leu Glu Glu 
1               5               


<210>  59
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  59

Gly Thr Lys Pro Ala Leu Glu Glu 
1               5               


<210>  60
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  60

Gly Thr Lys Pro Pro Ala Glu Glu 
1               5               


<210>  61
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  61

Gly Thr Lys Pro Pro Leu Ala Glu 
1               5               


<210>  62
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  62

Gly Thr Lys Pro Pro Leu Glu Ala 
1               5               


<210>  63
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  63

Gly Thr Lys Pro Pro Leu Glu Glu 
1               5               


<210>  64
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  64

Gly Thr Lys Pro Pro Leu Ala 
1               5           


<210>  65
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  65

Gly Thr Lys Pro Ala Ala Ala 
1               5           


<210>  66
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  66

Gly Thr Lys Ala Ala Ala Ala 
1               5           


<210>  67
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  67

Gly Thr Ala Ala Ala Ala Ala 
1               5           


<210>  68
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  68

Ala Thr Ala Ala Ala Ala Ala 
1               5           


<210>  69
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  69

Gly Thr Lys Ala Ala Ala 
1               5       


<210>  70
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  70

Gly Thr Lys Ala Ala 
1               5   


<210>  71
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  71

Gly Thr Lys Ala 
1               


<210>  72
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  72

Gly Thr Lys Pro Pro Leu Glu Glu Leu 
1               5                   


<210>  73
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  mod_res
<222>  (2)..(2)
<223>  Tn Modified

<400>  73

Gly Thr Glu Ser Pro Val Arg Glu 
1               5               


<210>  74
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  mod_res
<222>  (2)..(2)
<223>  Tn Modified

<400>  74

Ser Thr Ala Pro Pro Ala His Gly 
1               5               


<210>  75
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  mod_res
<222>  (2)..(2)
<223>  Tn modified

<400>  75

Ser Thr Pro Pro Ser Val Thr Ser 
1               5               


<210>  76
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Tn Modified

<400>  76

Ser Thr Lys Ala Glu His Leu Thr 
1               5               


<210>  77
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  mod_res
<222>  (2)..(2)
<223>  Tn Modified

<400>  77

Glu Thr Ser Lys Gly Thr Ser Gly 
1               5               


<210>  78
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  mod_res
<222>  (2)..(2)
<223>  mod_res

<400>  78

Ser Thr Gln Pro Thr Ser Thr Val 
1               5               


<210>  79
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Tn Modified

<400>  79

Gly Thr Ser Ala Ser Leu Val Tyr 
1               5               


<210>  80
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Tn Modified

<400>  80

Thr Thr Ala Pro Pro Ala Pro Pro 
1               5               


