                         SEQUENCE LISTING

<110>  T2 Biosystems, Inc.
 
<120>  METHODS AND COMPOSITIONS FOR HIGH SENSITIVITY SEQUENCING IN 
       COMPLEX SAMPLES

<130>  50713-125WO3

<150>  US 62/860,907
<151>  2019-06-13

<150>  US 62/729,373
<151>  2018-09-10

<160>  101   

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  1
cgttttccaa atctgtaaca gactggg                                           27


<210>  2
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2
aggacgttga taggttggat gtgga                                             25


<210>  3
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  3
ggtagctatg tagggaaggg ataaacgctg a                                      31


<210>  4
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4
gcgctaagga gcttaacttc tgtgttcg                                          28


<210>  5
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  5
gacggttgtc ccggtttaag ca                                                22


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6
gctggtatct tcgactggtc t                                                 21


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  7
aggctgggtg tgtaagcgtt gt                                                22


<210>  8
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8
caagcaattc ggttggatat ccgtt                                             25


<210>  9
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is 2,6-Diaminopurine

<400>  9
ggtaatgaat tacctntctc tgctggtttc ttctt                                  35


<210>  10
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is 2,6-Diaminopurine

<400>  10
accagcatct tcngcatctt ctgtaaa                                           27


<210>  11
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is 2,6-Diaminopurine

<400>  11
gaagttatgt ttnctattcg aatcgtggtc cagt                                   34


<210>  12
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12
gttgtaaagc catgatgctc gtaacca                                           27


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13
ggcatgcctg tttgagcgtc                                                   20


<210>  14
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14
gcttattgat atgcttaagt tcagcgggt                                         29


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15
tgaggcttga ctatacaaca cc                                                22


<210>  16
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16
ctaaaatgaa cagataaagt aagattcaa                                         29


<210>  17
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  17
aaaacttata tgacttcaaa tccagtttt                                         29


<210>  18
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  18
tttactcaat aaaagataac accacag                                           27


<210>  19
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  19
tggataagta aaagcaactt ggtt                                              24


<210>  20
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  20
aatgaagatt caactcaata agaaacaaca                                        30


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  21
taccaaggcg cttgagagaa ctc                                               23


<210>  22
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  22
ctggtgtgta ggtgaagtc                                                    19


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  23
gtgtgttgta gggtgaagtc gac                                               23


<210>  24
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  24
caccttgaaa tcacatacct ga                                                22


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  25
ccatttgaag ttgtttatta tgc                                               23


<210>  26
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  26
gggaaatgat taattatgca ttaaatc                                           27


<210>  27
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  27
tttttcagat ttaggattag ttgatt                                            26


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  28
gatccgtatt ggttatatca tc                                                22


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  29
ggaataatac gccgaccagc                                                   20


<210>  30
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  30
aaggatctat ttcagtatga tgcag                                             25


<210>  31
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  31
tgccgaagcg ttttccaaat ctgtaacaga ctgggctgat tgaatcttac tttatctgtt       60

cattttagct agaggtataa ctaaatcaag ttgtcttgca tatttaagaa tcgattgatg      120

ctttatatac aactgcttgg gtgttgtata gtcaagcctc acgagcaatt agtattggtc      180

agcttcacat atcactatgc                                                  200


<210>  32
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  32
gcatgggaac aggtgtatcc ttctcgctat cgccaccaca ctgggtgttg tttcttattg       60

agttgaatct tcattcactc aaaactggat tgaagtttga atcaaaataa ccaagttgct      120

tttacttatc cattctttgg ttaagtcctc gaccgattag tattggtccg ctccaactat      180

cactagcctt ccacttccaa                                                  200


<210>  33
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  33
gcatggttac aggtgtatcc ttctcgctat cgccaccaca ctgtggtgtt atcttttatt       60

gagtaaattt tgttcactca aaactggatt tgaagtcata taagtttttt tccgagttct      120

tttcttttaa cctattggtt aagtcctcga tcgattagta tcagtccgct ccatacatca      180

ctgtacttcc actcctgacc                                                  200


<210>  34
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  34
cagctccatc cgcagggact tcacctacac accagcgtgc cttctcccga agttacggca       60

ccattttgcc tagttccttc acccgagttc tctcaagcgc cttggtattc tctacctgac      120

cacctgtgtc ggtttggggt acgatttgat gttacctgat gcttagaggc ttttcctgga      180

agcagggcat ttgttacttc                                                  200


<210>  35
<211>  169
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  35
cgcttgggct tacgtctatc cggattcagg tatgtgattt caaggtgttt tgcggttcat       60

gcgaactttc ggttcgtcga cttcacctta caacacacaa tcgtcagatt gtttgggtgt      120

tatatggtca agcctcacgg gcaattagta ctggttagct caacgcctc                  169


<210>  36
<211>  151
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  36
tttaccacta acaccataga aattataacg gtcaatgcca tgatttaatg cataattaat       60

catttcccat tgcactgcat aacttccggc aaaatgacgg aatgcatttg atgtaccacc      120

agcataataa acaacttcaa atgggttgat a                                     151


<210>  37
<211>  186
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  37
tgtgatttaa acaagtttac taaggcatca tttttctcgc gaccttcaaa tggcacgata       60

tctttatcat atagatgata taaccaatac ggatctaatt taacatataa acattgatgt      120

tgctgtaaat atttatctaa ctcttttaaa taataatcaa ctaatcctaa atctgaaaaa      180

tccatt                                                                 186


<210>  38
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  38
gcattaatcg acggtatggt tgacc                                             25


<210>  39
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  39
cctgctgaaa caggttttcc cacata                                            26


<210>  40
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  40
agtgatgatg agttgtttgc cagtg                                             25


<210>  41
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  41
tgaattgtcg ccgcgtgacc ag                                                22


<210>  42
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  42
acccagcggt ttgagggaga aac                                               23


<210>  43
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  43
aaagtttgaa gatatacgtg gtggacgtta                                        30


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  44
cgcacgcgca agatggaaac g                                                 21


<210>  45
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  45
aagttcagcg ggtattccta cct                                               23


<210>  46
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  46
agctttttgt tgtctcgcaa cactcgc                                           27


<210>  47
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  47
ctaccaaaca caatgtgttt gagaag                                            26


<210>  48
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  48
cctgatttga ggtcaaactt aaagacgtct g                                      31


<210>  49
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is 5' 5-Nitroindole

<400>  49
agtcctacct gatttgaggt cnaa                                              24


<210>  50
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is 5' 5-Nitroindole

<400>  50
ccgngggttt gagggagaaa t                                                 21


<210>  51
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  51
aaagttatga aataaattgt ggtggccact agc                                    33


<210>  52
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  52
acccgggggt ttgagggaga aa                                                22


<210>  53
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  53
agtcctacct gatttgaggt cgaa                                              24


<210>  54
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  54
ccgagggttt gagggagaaa t                                                 21


<210>  55
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  55
ggaagggatc aggtggttca ctctt                                             25


<210>  56
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  56
aaaacttatg tgacttcaaa tccagtttt                                         29


<210>  57
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  57
tctgacgatt gtgtgttgta agg                                               23


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  58
ggatagacgt aagcccaagc                                                   20


<210>  59
<211>  198
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  59
gcatggttac aggtgtatcc ttctcgctat cgccaccaca ctgtggtgtt atcttttatt       60

gagtaaattt tgttcactca aaactggatt tgaagtcata taagtttttt tccgagttct      120

tttcttttaa cctattggtt aagtcctcga tcgattagta tcagtccgct ccatacatca      180

ctgtacttcc actcctga                                                    198


<210>  60
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  60
ggttaagtcc cgcaacgagc gc                                                22


<210>  61
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  61
aggaggtgat ccaaccgca                                                    19


<210>  62
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  62
gggcatgcct gtttgagcgt                                                   20


<210>  63
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  63
ctacctgatt tgaggcgaca acaaaac                                           27


<210>  64
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  64
ccgcgaagat tggtgagaag acat                                              24


<210>  65
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  65
cctacctgat ttgagggcga aatgtc                                            26


<210>  66
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  66
ggagcaacgc ctaaccggg                                                    19


<210>  67
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  67
gtcctacctg atttgagggg aaaaagc                                           27


<210>  68
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  68
aacaaatcca ccaacggtga gaagatat                                          28


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  69
gcgtagactt cgctgcggat                                                   20


<210>  70
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  70
cgtagacttc gctgcggat                                                    19


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  71
ctgggcggtg agaagaaatc                                                   20


<210>  72
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  72
gcgtagactt cgctgctgga a                                                 21


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  73
ccgtgcggtg agaagaaatc                                                   20


<210>  74
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  74
tcctacctga tttgaggaaa tagcatgg                                          28


<210>  75
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  75
atttagcgga tgcaaaacca cc                                                22


<210>  76
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  76
ggaaatctaa cgagagagca tgct                                              24


<210>  77
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  77
cgatgcgtga cacccaggc                                                    19


<210>  78
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  78
caaggtgcaa tgactttgtt tgggca                                            26


<210>  79
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  79
gcaacttcaa agtgtacagt attggtatcc c                                      31


<210>  80
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  80
agctgtagtt taaggcaaat gttgg                                             25


<210>  81
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  81
aggatcgcaa aatcaaccac aaaca                                             25


<210>  82
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  82
cctaaatgtt aaaccccttg acaaccca                                          28


<210>  83
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  83
cccatcagga tatccagctt cgg                                               23


<210>  84
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  84
ctttaccgat acttcaattt caccgagctc ca                                     32


<210>  85
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  85
cacaggtctc tgcaaatctg taaagagaa                                         29


<210>  86
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  86
cgatggttca cgggattctg caattc                                            26


<210>  87
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  87
gagacgtttt ggatacatgt gaaagaaggc                                        30


<210>  88
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  88
ttgtagaaca atctgggctt tttgg                                             25


<210>  89
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  89
ggagaactca tatcaggagc acaa                                              24


<210>  90
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  90
gctatttctg ctgttaaaag ttcttgt                                           27


<210>  91
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  91
ctaaaactta agctttgcaa ttgtgg                                            26


<210>  92
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  92
tctagcggtt gacagagaaa cattg                                             25


<210>  93
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  93
aaaaaattaa aaccatataa cccacgaa                                          28


<210>  94
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  94
gagacagcgt ccaaatcgtt acacc                                             25


<210>  95
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  95
tcttaacctt ccagcaccgg gca                                               23


<210>  96
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  96
ctatgtaggg aagggataaa cgctga                                            26


<210>  97
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  97
gcgctaagga gcttaacttc tgtgttcg                                          28


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  98
ctcctacggg aggcagcagt                                                   20


<210>  99
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  99
gtattaccgc ggctgctggc a                                                 21


<210>  100
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  100
tcgtcggcag cgtcagatgt gtataagaga cagctcctac gggaggcagc agt              53


<210>  101
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  101
gtctcgtggg ctcggagatg tgtataagag acaggtatta ccgcggctgc tggca            55


