               SEQUENCE LISTING

<110> ISTANBUL MEDIPOL UNIVERSITESI

<120> METHOD FOR PREPARING RECOMBINANT PETase AND MHETase ENZYMES
FOR USE IN DECOMPOSITION OF PLASTICS

<130> MedipolPCT33

<140> TRPCT/TR2019/050737
<141> 2019-09-09

<150> TR 2018/12953
<151> 2018-09-11

<160> 85

<170> BiSSAP 1.3.6

<210> 1
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<223> Signal Peptide

<400> 1
Met Tyr Gln Leu Leu Ser Val Ala Ser Val Pro Leu Leu Ala Ser Leu 
1               5                   10                  15      
Val His Gly 
            

<210> 2
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> Signal Peptide

<400> 2
Met Phe Ala Lys Arg Phe Lys Thr Ser Leu Leu Pro Leu Phe Ala Gly 
1               5                   10                  15      
Phe Leu Leu Leu Phe His Leu Val Leu Ala Gly 
            20                  25          

<210> 3
<211> 29
<212> PRT
<213> Artificial Sequence


<220> 
<223> Signal peptide

<400> 3
Met Arg Ser Lys Lys Leu Trp Ile Ser Leu Leu Phe Ala Leu Thr Leu 
1               5                   10                  15      
Ile Phe Thr Met Ala Phe Ser Asn Met Ser Ala Gln Ala 
            20                  25                  

<210> 4
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> Signal protein

<400> 4
Met Asn Met Gln Ile Thr Lys Ile Leu Asn Asn Asn Val Val Val Val 
1               5                   10                  15      
Ile Asp Asp Gln 
            20  

<210> 5
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> Signal peptide

<400> 5
Met Pro Tyr Leu Lys Arg Val Leu Leu Leu Leu Val Thr Gly Leu Phe 
1               5                   10                  15      
Met Ser Leu Phe Ala Val Thr Ala Thr Ala Ser Ala 
            20                  25              

<210> 6
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> Signal peptide

<400> 6
Met Arg Lys Lys Thr Lys Asn Arg Leu Ile Ser Ser Val Leu Ser Thr 
1               5                   10                  15      
Val Val Ile Ser Ser Leu Leu Phe Pro Gly Ala Ala Gly Ala 
            20                  25                  30  

<210> 7
<211> 42
<212> PRT
<213> Artificial Sequence


<220> 
<223> Signal peptide

<400> 7
Met His Met Ser Asn Ala Arg Pro Ser Lys Ser Arg Thr Lys Phe Leu 
1               5                   10                  15      
Leu Ala Phe Leu Cys Phe Thr Leu Met Ala Ser Leu Phe Gly Ala Thr 
            20                  25                  30          
Ala Leu Phe Gly Pro Ser Lys Ala Ala Ala 
        35                  40          

<210> 8
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> Signal Peptide

<400> 8
Met Lys Asn Met Ser Cys Lys Leu Val Val Ser Val Thr Leu Phe Phe 
1               5                   10                  15      
Ser Phe Leu Thr Ile Gly Pro Leu Ala His Ala 
            20                  25          

<210> 9
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 9
Met Lys Arg Thr Trp Asn Val Cys Leu Thr Ala Leu Leu Ser Val Leu 
1               5                   10                  15      
Leu Val Ala Gly Ser Val Pro Phe His Ala Glu Ala 
            20                  25              

<210> 10
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 10
Met Leu Asn Ile Phe Lys Pro Ala Pro His Ile Glu Arg Leu Asp Asp 
1               5                   10                  15      
Ser Lys Met Asp Ala Ala Tyr Lys Arg Leu Arg Leu Gln Val 
            20                  25                  30  

<210> 11
<211> 26
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 11
Met Gly Tyr Tyr Asp Gly Pro Asn Leu Asn Glu Glu His Ser Glu Thr 
1               5                   10                  15      
Arg Glu Val Arg Lys Ser Gly Ser Lys Lys 
            20                  25      

<210> 12
<211> 31
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 12
Met Lys Phe Val Lys Arg Arg Ile Ile Ala Leu Val Thr Ile Leu Met 
1               5                   10                  15      
Leu Ser Val Thr Ser Leu Phe Ala Leu Gln Pro Ser Ala Lys Ala 
            20                  25                  30      

<210> 13
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 13
Met Lys Lys Arg Leu Ile Ala Pro Met Leu Leu Ser Ala Ala Ser Leu 
1               5                   10                  15      
Ala Phe Phe Ala Met Ser Gly Ser Ala Gln Ala 
            20                  25          

<210> 14
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 14
Met Arg Gln Gly Leu Met Ala Ala Val Leu Phe Ala Thr Phe Ala Leu 
1               5                   10                  15      
Thr Gly 
        

<210> 15
<211> 43
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 15
Met Thr Leu Lys Glu Gly Asp Asp Lys Met Lys Leu Val Pro Arg Phe 
1               5                   10                  15      
Arg Lys Gln Trp Phe Ala Tyr Leu Thr Val Leu Cys Leu Ala Leu Ala 
            20                  25                  30          
Ala Ala Val Ser Phe Gly Val Pro Ala Lys Ala 
        35                  40              

<210> 16
<211> 24
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 16
Ala Ala Ala Thr Gly Ser Gly Thr Thr Leu Lys Gly Ala Thr Val Pro 
1               5                   10                  15      
Leu Asn Ile Ser Tyr Glu Gly Gly 
            20                  

<210> 17
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 17
Met Lys Lys Arg Trp Ser Ile Val Thr Leu Met Leu Ile Phe Thr Leu 
1               5                   10                  15      
Val Leu Ser Ala 
            20  

<210> 18
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 18
Phe Thr Leu Leu Ile Leu Phe Val Leu Gly Val Val Gly Gly Ala Val 
1               5                   10                  15      
Thr Phe Ala Val 
            20  

<210> 19
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 19
Met Thr Ser Pro Thr Arg Arg Arg Thr Ala Lys Arg Arg Arg Arg Lys 
1               5                   10                  15      
Leu Asn Lys Arg 
            20  

<210> 20
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 20
Met Lys Lys Val Met Leu Ala Thr Ala Leu Phe Leu Gly Leu Thr Pro 
1               5                   10                  15      
Ala Gly Ala Asn Ala 
            20      

<210> 21
<211> 24
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 21
Met Lys Arg Phe Cys Leu Trp Phe Ala Val Phe Ser Leu Leu Leu Val 
1               5                   10                  15      
Leu Leu Pro Gly Lys Ala Phe Gly 
            20                  

<210> 22
<211> 36
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 22
Met Lys Leu Lys Thr Lys Ala Ser Ile Lys Phe Gly Ile Cys Val Gly 
1               5                   10                  15      
Leu Leu Cys Leu Ser Ile Thr Gly Phe Thr Pro Phe Phe Asn Ser Thr 
            20                  25                  30          
His Ala Glu Ala 
        35      

<210> 23
<211> 41
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 23
Met Lys Lys Met Ser Leu Phe Gln Asn Met Lys Ser Lys Leu Leu Pro 
1               5                   10                  15      
Ile Ala Ala Val Ser Val Leu Thr Ala Gly Ile Phe Ala Gly Ala Glu 
            20                  25                  30          
Leu Gln Gln Thr Glu Lys Ala Ser Ala 
        35                  40      

<210> 24
<211> 32
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 24
Met Lys Lys Phe Pro Lys Lys Leu Leu Pro Ile Ala Val Leu Ser Ser 
1               5                   10                  15      
Ile Ala Phe Ser Ser Leu Ala Ser Gly Ser Val Pro Glu Ala Ser Ala 
            20                  25                  30          



<210> 25
<211> 56
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 25
Met Ala Tyr Asp Ser Arg Phe Asp Glu Trp Val Gln Lys Leu Lys Glu 
1               5                   10                  15      
Glu Ser Phe Gln Asn Asn Thr Phe Asp Arg Arg Lys Phe Ile Gln Gly 
            20                  25                  30          
Ala Gly Lys Ile Ala Gly Leu Ser Leu Gly Leu Thr Ile Ala Gln Ser 
        35                  40                  45              
Val Gly Ala Phe Glu Val Asn Ala 
    50                  55      

<210> 26
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 26
Met Lys Lys Asn Lys Leu Val Leu Met Leu Leu Met Ala Ala Phe Met 
1               5                   10                  15      
Met Ile Ala Ala Ala 
            20      

<210> 27
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 27
Met Gly Met Lys Lys Lys Leu Ser Leu Gly Val Ala Ser Ala Ala Leu 
1               5                   10                  15      
Gly Leu Ala Leu Val Gly Gly Gly Thr Trp Ala 
            20                  25          

<210> 28
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 28
Met Lys Lys Gly Ile Ile Arg Phe Leu Leu Val Ser Phe Val Leu Phe 
1               5                   10                  15      
Phe Ala Leu Ser Thr Gly Ile Thr Gly Val Gln Ala 
            20                  25              

<210> 29
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 29
Met Lys Lys Arg Lys Arg Arg Asn Phe Lys Arg Phe Ile Ala Ala Phe 
1               5                   10                  15      
Leu Val Leu Ala Leu Met Ile Ser Leu Val Pro Ala 
            20                  25              

<210> 30
<211> 31
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 30
Met Lys Arg Arg Lys Phe Ser Ser Val Val Ala Ala Val Leu Ile Phe 
1               5                   10                  15      
Ala Leu Ile Phe Ser Leu Phe Ser Pro Gly Thr Lys Ala Ala Ala 
            20                  25                  30      

<210> 31
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 31
Met Ile Thr Leu Phe Arg Lys Pro Phe Val Ala Gly Leu Ala Ile Ser 
1               5                   10                  15      
Leu Leu Val Gly Gly Gly Ile Gly Asn Val Ala Ala 
            20                  25              

<210> 32
<211> 26
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 32
Met Arg Lys Lys Cys Ser Val Cys Leu Trp Ile Leu Val Leu Leu Leu 
1               5                   10                  15      
Ser Cys Leu Ser Gly Lys Ser Ala Tyr Ala 
            20                  25      

<210> 33
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 33
Met Val Lys Lys Trp Leu Ile Gln Phe Ala Val Met Leu Ser Val Leu 
1               5                   10                  15      
Ser Thr Phe Thr Tyr Ser Ala Ser Ala 
            20                  25  

<210> 34
<211> 23
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 34
Met Lys Lys Trp Ile Tyr Val Val Leu Val Leu Ser Ile Ala Gly Ile 
1               5                   10                  15      
Gly Gly Phe Ser Val His Ala 
            20              

<210> 35
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 35
Met Phe Lys Lys Trp Ser Gly Leu Phe Val Ile Ala Ala Cys Phe Leu 
1               5                   10                  15      
Leu Val Ala Ala 
            20  

<210> 36
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 36
Met Lys Lys Phe Ala Leu Leu Phe Ile Ala Leu Val Thr Ala Val Val 
1               5                   10                  15      
Ile Ser Ala 
            

<210> 37
<211> 23
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 37
Met Arg Arg Ile Leu Ser Ile Leu Val Phe Ala Ile Met Leu Ala Gly 
1               5                   10                  15      
Cys Ser Ser Asn Ala Ser Thr 
            20              

<210> 38
<211> 26
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 38
Met Phe Lys Lys His Thr Ile Ser Leu Leu Ile Ile Phe Leu Leu Ala 
1               5                   10                  15      
Ser Ala Val Leu Ala Lys Pro Ile Glu Ala 
            20                  25      

<210> 39
<211> 35
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 39
Met Arg Ile Gln Lys Arg Arg Thr His Val Glu Asn Ile Leu Arg Ile 
1               5                   10                  15      
Leu Leu Pro Pro Ile Met Ile Leu Ser Leu Ile Leu Pro Thr Pro Pro 
            20                  25                  30          
Ile His Ala 
        35  

<210> 40
<211> 23
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 40
Met Thr Ser Ile His Glu Asp Thr His Ile Gly Tyr Ala Lys Leu Thr 
1               5                   10                  15      
Ile Arg Ser Leu Glu Arg Ser 
            20              

<210> 41
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 41
Met Arg Thr Tyr Ser Asn Lys Leu Ile Ala Ile Met Ser Val Leu Leu 
1               5                   10                  15      
Leu Ala 
        

<210> 42
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 42
Met Lys Lys Leu Phe Ser Tyr Arg Leu Ser Phe Phe Val Leu Ala Ile 
1               5                   10                  15      
Ile Leu Phe Trp Ala 
            20      

<210> 43
<211> 46
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 43
Met Leu Ser Val Glu Met Ile Ser Arg Gln Asn Arg Cys His Tyr Val 
1               5                   10                  15      
Tyr Lys Gly Gly Asn Met Met Arg Arg Ile Leu His Ile Val Leu Ile 
            20                  25                  30          
Thr Ala Leu Met Phe Leu Asn Val Met Tyr Thr Phe Glu Ala 
        35                  40                  45      

<210> 44
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 44
Met Lys Lys Ile Gly Leu Leu Phe Met Leu Cys Leu Ala Ala Leu Phe 
1               5                   10                  15      
Thr Ile Gly Phe Pro Ala Gln Gln Ala Asp Ala 
            20                  25          

<210> 45
<211> 31
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 45
Met Lys Arg Arg Lys Phe Ser Ser Val Val Ala Ala Val Leu Ile Phe 
1               5                   10                  15      
Ala Leu Ile Phe Ser Leu Phe Ser Pro Gly Thr Lys Ala Ala Ala 
            20                  25                  30      

<210> 46
<211> 32
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 46
Met Ile Pro Arg Ile Lys Lys Thr Ile Cys Val Leu Leu Val Cys Phe 
1               5                   10                  15      
Thr Met Leu Ser Val Met Leu Gly Pro Gly Ala Thr Glu Val Leu Ala 
            20                  25                  30          



<210> 47
<211> 24
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 47
Met Lys Lys Met Leu Met Leu Ala Phe Thr Phe Leu Leu Ala Leu Thr 
1               5                   10                  15      
Ile His Val Gly Glu Ala Ser Ala 
            20                  

<210> 48
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 48
Met Asn Lys Val Gly Phe Phe Arg Ser Ile Gln Phe Lys Ile Thr Leu 
1               5                   10                  15      
Ile Tyr Val 
            

<210> 49
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 49
Met Lys Lys Arg Ile Thr Tyr Ser Leu Leu Ala Leu Leu Ala Val Val 
1               5                   10                  15      
Ala Phe Ala Phe Thr Asp Ser Ser Lys Ala Lys 
            20                  25          

<210> 50
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 50
Met Lys Lys Trp Leu Leu Leu Leu Val Ala Ala Cys Ile Thr Phe Ala 
1               5                   10                  15      
Leu Thr Ala 
            

<210> 51
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 51
Met Phe Lys Lys Leu Leu Leu Ala Thr Ser Ala Leu Thr Phe Ser Leu 
1               5                   10                  15      
Ser Leu 
        

<210> 52
<211> 47
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 52
Met Ala Lys Pro Leu Ser Lys Gly Gly Ile Leu Val Lys Lys Val Leu 
1               5                   10                  15      
Ile Ala Gly Ala Val Gly Thr Ala Val Leu Phe Gly Thr Leu Ser Ser 
            20                  25                  30          
Gly Ile Pro Gly Leu Pro Ala Ala Asp Ala Gln Val Ala Lys Ala 
        35                  40                  45          

<210> 53
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 53
Met Asn Ile Leu Phe Ser Lys Arg Leu Gly Ile Leu Thr Ile Gly Ser 
1               5                   10                  15      
Leu Leu Val Leu Ala Gly Cys Gln Thr Ser Gly Ser Ser Ala 
            20                  25                  30  

<210> 54
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 54
Met Lys Arg Lys Leu Leu Ser Ser Leu Ala Ile Ser Ala Leu Ser Leu 
1               5                   10                  15      
Gly Leu Leu Val Ser Ala Pro Thr Ala Ser Phe Ala 
            20                  25              

<210> 55
<211> 26
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 55
Met Thr Lys Lys Ala Trp Phe Leu Pro Leu Val Cys Val Leu Leu Ile 
1               5                   10                  15      
Ser Gly Trp Leu Ala Pro Ala Ala Ser Ala 
            20                  25      

<210> 56
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 56
Met Arg Lys Ser Leu Ile Thr Leu Gly Leu Ala Ser Val Ile Gly Thr 
1               5                   10                  15      
Ser Ser Phe Leu Ile Pro Phe Thr Ser Lys Thr Ala Ser Ala 
            20                  25                  30  

<210> 57
<211> 26
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 57
Met Lys Arg Ile Arg Ile Pro Met Thr Leu Ala Leu Gly Ala Ala Leu 
1               5                   10                  15      
Thr Ile Ala Pro Leu Ser Phe Ala Ser Ala 
            20                  25      

<210> 58
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 58
Met Lys Lys Ile Val Ser Ile Leu Phe Met Phe Gly Leu Val Met Gly 
1               5                   10                  15      
Phe Ser Gln Phe Gln Pro Ser Thr Val Phe Ala 
            20                  25          

<210> 59
<211> 31
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 59
Met Lys Lys Leu Leu Thr Val Met Thr Met Ala Val Leu Thr Ala Gly 
1               5                   10                  15      
Thr Leu Leu Leu Pro Ala Gln Ser Val Thr Pro Ala Ala His Ala 
            20                  25                  30      

<210> 60
<211> 31
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 60
Met Lys Arg Arg Lys Phe Ser Ser Val Val Ala Ala Val Leu Ile Phe 
1               5                   10                  15      
Ala Leu Ile Phe Ser Leu Phe Ser Pro Gly Thr Lys Ala Ala Ala 
            20                  25                  30      

<210> 61
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 61
Met Arg Lys Trp Tyr Phe Ile Leu Leu Ala Gly Val Leu Thr Ser Val 
1               5                   10                  15      
Ile Leu Ala Phe Val Tyr Asp Lys Thr Lys Ala 
            20                  25          

<210> 62
<211> 32
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 62
Met Asn Thr Leu Ala Asn Trp Lys Lys Phe Leu Leu Val Ala Val Ile 
1               5                   10                  15      
Ile Cys Phe Leu Val Pro Ile Met Thr Lys Ala Glu Ile Ala Glu Ala 
            20                  25                  30          



<210> 63
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 63
Val Val Ala Gly Leu Met Ala Phe Leu Val Ile Ala Ala Gly Ser Val 
1               5                   10                  15      
Gly Ala Tyr Ala Phe 
            20      

<210> 64
<211> 41
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 64
Met Val Lys Ser Phe Arg Met Lys Ala Leu Ile Ala Gly Ala Ala Val 
1               5                   10                  15      
Ala Ala Ala Val Ser Ala Gly Ala Val Ser Asp Val Pro Ala Ala Lys 
            20                  25                  30          
Val Leu Gln Pro Thr Ala Ala Tyr Ala 
        35                  40      

<210> 65
<211> 26
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 65
Met Phe Asn Arg Leu Phe Arg Val Cys Phe Leu Ala Ala Leu Ile Met 
1               5                   10                  15      
Ala Phe Thr Leu Pro Asn Ser Val Tyr Ala 
            20                  25      

<210> 66
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 66
Met Arg His Lys Ile Ile Thr Phe Ile Leu Ala Val Val Val Ile Ile 
1               5                   10                  15      
Ile Ile Gly Asn Met Ile Gly Gly Gly Gly Gly Ser Glu Ala 
            20                  25                  30  

<210> 67
<211> 603
<212> PRT
<213> Artificial Sequence


<220> 
<223> MHETase

<400> 67
Met Gln Thr Thr Val Thr Thr Met Leu Leu Ala Ser Val Ala Leu Ala 
1               5                   10                  15      
Ala Cys Ala Gly Gly Gly Ser Thr Pro Leu Pro Leu Pro Gln Gln Gln 
            20                  25                  30          
Pro Pro Gln Gln Glu Pro Pro Pro Pro Pro Val Pro Leu Ala Ser Arg 
        35                  40                  45              
Ala Ala Cys Glu Ala Leu Lys Asp Gly Asn Gly Asp Met Val Trp Pro 
    50                  55                  60                  
Asn Ala Ala Thr Val Val Glu Val Ala Ala Trp Arg Asp Ala Ala Pro 
65                  70                  75                  80  
Ala Thr Ala Ser Ala Ala Ala Leu Pro Glu His Cys Glu Val Ser Gly 
                85                  90                  95      
Ala Ile Ala Lys Arg Thr Gly Ile Asp Gly Tyr Pro Tyr Glu Ile Lys 
            100                 105                 110         
Phe Arg Leu Arg Met Pro Ala Glu Trp Asn Gly Arg Phe Phe Met Glu 
        115                 120                 125             
Gly Gly Ser Gly Thr Asn Gly Ser Leu Ser Ala Ala Thr Gly Ser Ile 
    130                 135                 140                 
Gly Gly Gly Gln Ile Ala Ser Ala Leu Ser Arg Asn Phe Ala Thr Ile 
145                 150                 155                 160 
Ala Thr Asp Gly Gly His Asp Asn Ala Val Asn Asp Asn Pro Asp Ala 
                165                 170                 175     
Leu Gly Thr Val Ala Phe Gly Leu Asp Pro Gln Ala Arg Leu Asp Met 
            180                 185                 190         
Gly Tyr Asn Ser Tyr Asp Gln Val Thr Gln Ala Gly Lys Ala Ala Val 
        195                 200                 205             
Ala Arg Phe Tyr Gly Arg Ala Ala Asp Lys Ser Tyr Phe Ile Gly Cys 
    210                 215                 220                 
Ser Glu Gly Gly Arg Glu Gly Met Met Leu Ser Gln Arg Phe Pro Ser 
225                 230                 235                 240 
His Tyr Asp Gly Ile Val Ala Gly Ala Pro Gly Tyr Gln Leu Pro Lys 
                245                 250                 255     
Ala Gly Ile Ser Gly Ala Trp Thr Thr Gln Ser Leu Ala Pro Ala Ala 
            260                 265                 270         
Val Gly Leu Asp Ala Gln Gly Val Pro Leu Ile Asn Lys Ser Phe Ser 
        275                 280                 285             
Asp Ala Asp Leu His Leu Leu Ser Gln Ala Ile Leu Gly Thr Cys Asp 
    290                 295                 300                 
Ala Leu Asp Gly Leu Ala Asp Gly Ile Val Asp Asn Tyr Arg Ala Cys 
305                 310                 315                 320 
Gln Ala Ala Phe Asp Pro Ala Thr Ala Ala Asn Pro Ala Asn Gly Gln 
                325                 330                 335     
Ala Leu Gln Cys Val Gly Ala Lys Thr Ala Asp Cys Leu Ser Pro Val 
            340                 345                 350         
Gln Val Thr Ala Ile Lys Arg Ala Met Ala Gly Pro Val Asn Ser Ala 
        355                 360                 365             
Gly Thr Pro Leu Tyr Asn Arg Trp Ala Trp Asp Ala Gly Met Ser Gly 
    370                 375                 380                 
Leu Ser Gly Thr Thr Tyr Asn Gln Gly Trp Arg Ser Trp Trp Leu Gly 
385                 390                 395                 400 
Ser Phe Asn Ser Ser Ala Asn Asn Ala Gln Arg Val Ser Gly Phe Ser 
                405                 410                 415     
Ala Arg Ser Trp Leu Val Asp Phe Ala Thr Pro Pro Glu Pro Met Pro 
            420                 425                 430         
Met Thr Gln Val Ala Ala Arg Met Met Lys Phe Asp Phe Asp Ile Asp 
        435                 440                 445             
Pro Leu Lys Ile Trp Ala Thr Ser Gly Gln Phe Thr Gln Ser Ser Met 
    450                 455                 460                 
Asp Trp His Gly Ala Thr Ser Thr Asp Leu Ala Ala Phe Arg Asp Arg 
465                 470                 475                 480 
Gly Gly Lys Met Ile Leu Tyr His Gly Met Ser Asp Ala Ala Phe Ser 
                485                 490                 495     
Ala Leu Asp Thr Ala Asp Tyr Tyr Glu Arg Leu Gly Ala Ala Met Pro 
            500                 505                 510         
Gly Ala Ala Gly Phe Ala Arg Leu Phe Leu Val Pro Gly Met Asn His 
        515                 520                 525             
Cys Ser Gly Gly Pro Gly Thr Asp Arg Phe Asp Met Leu Thr Pro Leu 
    530                 535                 540                 
Val Ala Trp Val Glu Arg Gly Glu Ala Pro Asp Gln Ile Ser Ala Trp 
545                 550                 555                 560 
Ser Gly Thr Pro Gly Tyr Phe Gly Val Ala Ala Arg Thr Arg Pro Leu 
                565                 570                 575     
Cys Pro Tyr Pro Gln Ile Ala Arg Tyr Lys Gly Ser Gly Asp Ile Asn 
            580                 585                 590         
Thr Glu Ala Asn Phe Ala Cys Ala Ala Pro Pro 
        595                 600             

<210> 68
<211> 290
<212> PRT
<213> Artificial Sequence


<220> 
<223> PETase

<400> 68
Met Asn Phe Pro Arg Ala Ser Arg Leu Met Gln Ala Ala Val Leu Gly 
1               5                   10                  15      
Gly Leu Met Ala Val Ser Ala Ala Ala Thr Ala Gln Thr Asn Pro Tyr 
            20                  25                  30          
Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu Ala Ser Ala Gly 
        35                  40                  45              
Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro Ser Gly Tyr Gly 
    50                  55                  60                  
Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly Thr Val Gly Ala 
65                  70                  75                  80  
Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser Ser Ile Lys Trp 
                85                  90                  95      
Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val Ile Thr Ile Asp 
            100                 105                 110         
Thr Asn Ser Thr Leu Asp Gln Pro Ser Ser Arg Ser Ser Gln Gln Met 
        115                 120                 125             
Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr Ser Ser Ser Pro 
    130                 135                 140                 
Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val Met Gly Trp Ser 
145                 150                 155                 160 
Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn Asn Pro Ser Leu 
                165                 170                 175     
Lys Ala Ala Ala Pro Gln Ala Pro Trp Asp Ser Ser Thr Asn Phe Ser 
            180                 185                 190         
Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu Asn Asp Ser Ile 
        195                 200                 205             
Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp Ser Met Ser Arg 
    210                 215                 220                 
Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser His Ser Cys Ala 
225                 230                 235                 240 
Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys Lys Gly Val Ala 
                245                 250                 255     
Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr Ser Thr Phe Ala 
            260                 265                 270         
Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe Arg Thr Ala Asn 
        275                 280                 285             
Cys Ser 
    290 

<210> 69
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> Marker Peptide - Avitag

<400> 69
Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  

<210> 70
<211> 26
<212> PRT
<213> Artificial Sequence


<220> 
<223> Marker tag - Calmodulin-tag

<400> 70
Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      
Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
            20                  25      

<210> 71
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> polyglutamate tag

<400> 71
Glu Glu Glu Glu Glu Glu 
1               5       

<210> 72
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> E-tag

<400> 72
Gly Ala Pro Val Pro Tyr Pro Asp Pro Leu Glu Pro Arg 
1               5                   10              

<210> 73
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> HA-tag

<400> 73
Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   

<210> 74
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Myc-tag

<400> 74
Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  

<210> 75
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> NE-tag

<400> 75
Thr Lys Glu Asn Pro Arg Ser Asn Gln Glu Glu Ser Tyr Asp Asp Asn 
1               5                   10                  15      
Glu Ser 
        

<210> 76
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> S-tag

<400> 76
Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser 
1               5                   10                  15  

<210> 77
<211> 38
<212> PRT
<213> Artificial Sequence


<220> 
<223> SBP-tag

<400> 77
Met Asp Glu Lys Thr Thr Gly Trp Arg Gly Gly His Val Val Glu Gly 
1               5                   10                  15      
Leu Ala Gly Glu Leu Glu Gln Leu Arg Ala Arg Leu Glu His His Pro 
            20                  25                  30          
Gln Gly Gln Arg Glu Pro 
        35              

<210> 78
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Softag 3

<400> 78
Thr Gln Asp Pro Ser Arg Val Gly 
1               5               

<210> 79
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spot tag

<400> 79
Pro Asp Arg Val Arg Ala Val Ser His Trp Ser Ser 
1               5                   10          

<210> 80
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Strep-tag

<400> 80
Trp Ser His Pro Gln Phe Glu Lys 
1               5               

<210> 81
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> TC tag

<400> 81
Cys Cys Pro Gly Cys Cys 
1               5       

<210> 82
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ty tag

<400> 82
Glu Val His Thr Asn Gln Asp Pro Leu Asp 
1               5                   10  

<210> 83
<211> 14
<212> PRT
<213> Artificial Sequence


<220> 
<223> V5 tag

<400> 83
Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  

<210> 84
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> VSV-tag

<400> 84
Tyr Thr Asp Ile Glu Met Asn Arg Leu Gly Lys 
1               5                   10      

<210> 85
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Xpress tag

<400> 85
Asp Leu Tyr Asp Asp Asp Asp Lys 
1               5               

