﻿               ПЕРЕЧЕНЬ ПОСЛЕДОВАТЕЛЬНОСТЕЙ

<110> ЗАКРЫТОЕ АКЦИОНЕРНОЕ ОБЩЕСТВО "БИОКАД"

<120> НУКЛЕАЗА PaCas9

<140> RU2018132816
<141> 2018-09-14

<160> 4

<170> BiSSAP 1.3.6

<210> 1
<211> 3135
<212> DNA
<213> Homoeodictya palmata


<400> 1
atggcaaaaa cacggcttgg tattgatatg gggactaatt ctatcggttg gatgttgtat      60

gaattggaca aaaacggtga aatttcggaa gttttaaagg caggggtgcg cattttccct     120

gatgggcgcg aggataaaac ccaagcatcc aaaaacgcgg cacggcgggt agcacgaatg     180

aatcgccgtc agcgagaccg ctatcttcag cgccggacag cgattttacg ctatttggta     240

aaatttgggc tgatgccaga agataaaaca gaacaacgaa aattgcagga tattgacccc     300

tatagtattc gtgccaaggc aatggaagaa gaaattccac cccatcatct tgggcgggca     360

atttttcata tcagccaaag acgtggctat aagagtagcc gtcgtaatga agaaaacgat     420

aaagatggtc cagtaaaaag ttccattgaa gaattccggc ggcagctggg cgacaaaagt     480

gtgggacaat ttttatctga attacaccaa gaaaataaac caatccgcgc ccgccgtgat     540

ggcgtaacta ataatgatct ttttcattat ttcccagacc gcgaactaat tgaaaaagag     600

tttaacgata tttggcaaaa acaacaacaa atccgctcgc aacaaccaga taaaaataaa     660

caaattttat ctgatatatt aacaaatgaa aataaacaaa ctttatttga agttattttt     720

catcagcgct ctttaaagcc gccaattatc ggcaattgtc aattttttcc gaccaaaaaa     780

agaatcgcta aggcgttgcc ttcctttcag cgattccgta ttttgcagga tattaataac     840

cttgaatatt ttgatgaaaa cgagtggcac cctttgccgt cgtctattcg tgattgggca     900

ttgcacattc tttttgcggg aggtaatcta acttttaaga agttaaatag ccaaatgaag     960

aaagatggtg aaattaacga atctgcattt tttaacttag aagatgaaaa acgcaatgat    1020

attaaaggcg actttaccac caaaaccatg aaagaaatta tccccgcatt gtgggataac    1080

tgggatttgc acaaacaaga cagcatgatt ttattactaa taggagataa aagtatagat    1140

gaagataaaa tgttagatga agataaaatg ttagatgaac tttcgtctca ttataattta    1200

tctgaagaag aggcacaaga atgtgcgaat gccaatattg acaacaggca aggcgttagt    1260

ggaagagcga gtttatcgct agaagcgata agcatactaa taccctattt ggaaaaagga    1320

aaccactatg ataaagcact tgctgagtcg ggaattgaaa aagataagtc ctctaaacat    1380

gatgaccatt tctatatgca gccctacccc gaaatattag ggcaatggtg tttgccgcgc    1440

aaaagcgagg atgaaagcaa caaagaattg tggcgtatcc cgaatccaac agtgcatgtt    1500

gcattaaatc aattgcgggc agttgttaac gactgcataa gaatcaatgg cggagaaaaa    1560

ccttcacaag ttgttgtgga gttggcacgt gacttgccaa taggtattgc aacacggcaa    1620

gaaattagaa aaaaacagtc agaaaatcaa agggcacgaa cacaacgccg caataaaatt    1680

gaagaactcg gagagcgtgc gagtgctaaa tctatgttgc gcatgcaatt gtgggaagaa    1740

tcgtctccca aaaatgccaa taatcactgt tgcctatatt gtggaaaaca aataggctgc    1800

gctgccgctg tctcttctcc cgattttgag attgaccata ttctaccttt ttctaaaact    1860

ttggatgata gcgccgccaa caaaacgctt tgctgcgtcc aatgcaaccg cgaaaaagga    1920

aataaaactc cttatgaagc atggggcagt aatgaaaaac ggtatgatga aattaaaacg    1980

agagcaagtg ccctatcgcc aaaaaaacga cggcgttttt tgccagacgc aatgaaacat    2040

tttgacggtg ataacgattt tctcgctcgc caactaaccg atacgcaata tatcgccaaa    2100

gtgacgaagc gttattttga atctattata aaacccaatg atgtttatgt tattcctggt    2160

cgtttaactg aaatattaag acgcaagtgg ggattgaata atattttgaa tgatgacgga    2220

cacaaaaacc gtgatgacca tagacatcat gccgttgatg cagcagtcat tggtgcgacc    2280

actcgttcta cgctccaaaa atttgctact gaggcaggca aagacaactc cgatttacca    2340

aatgtctcta tcaccgcacc aatgaaagta tttcgtgaaa aggtggaaaa gaccgtaaaa    2400

aatattgttg tctcacacaa accagatcgt tttgagagtg gcgcgttaca taatgatact    2460

gcttatgggt tgcctgctga ttataaagaa ggtgctggtg cacaattcgt aagacatcgg    2520

attatgttgt ctgatataac tacaaatttt tctcaaaaag tggttaattc tttcttgcgc    2580

gaagaattga aaacattatg cgactatgca acggataaaa aatctttaga tcaagaatta    2640

aaacgctatg gagaaaaaaa taatattcgc cgtgttttaa tagaagaaaa attgtctgtt    2700

attgctgttt gcgataagga cggcaaaccc tataaaggtt ataaaagcga cagtaattgg    2760

gcctatgaga tttttgagaa atttcccaat ggaaattggg atggcgaaat tatttccaca    2820

tttaatgcta atcaagaaaa atttacgccc ttatggaaag aaaaaaatcc caaggctaaa    2880

cttatcatgc gcttgcacaa aaatgacatg gtagcattag atgacaatgg actacgaaaa    2940

atatgttatg taaaaacatt aagtgctagc aaaattgcca tggttgaaca ttttgatgcc    3000

actcctggta gaaatccacc tactattata accaaaagtc caaacgaatt tcgcaaaatc    3060

aatggacgaa aaatccatat tagccctggc gggttagtaa gagacactaa aaaagatggc    3120

cgatcaaatc attga                                                     3135





<210> 2
<211> 1044
<212> PRT
<213> Homoeodictya palmata


<400> 2
Met Ala Lys Thr Arg Leu Gly Ile Asp Met Gly Thr Asn Ser Ile Gly 
1               5                   10                  15      
Trp Met Leu Tyr Glu Leu Asp Lys Asn Gly Glu Ile Ser Glu Val Leu 
            20                  25                  30          
Lys Ala Gly Val Arg Ile Phe Pro Asp Gly Arg Glu Asp Lys Thr Gln 
        35                  40                  45              
Ala Ser Lys Asn Ala Ala Arg Arg Val Ala Arg Met Asn Arg Arg Gln 
    50                  55                  60                  
Arg Asp Arg Tyr Leu Gln Arg Arg Thr Ala Ile Leu Arg Tyr Leu Val 
65                  70                  75                  80  
Lys Phe Gly Leu Met Pro Glu Asp Lys Thr Glu Gln Arg Lys Leu Gln 
                85                  90                  95      
Asp Ile Asp Pro Tyr Ser Ile Arg Ala Lys Ala Met Glu Glu Glu Ile 
            100                 105                 110         
Pro Pro His His Leu Gly Arg Ala Ile Phe His Ile Ser Gln Arg Arg 
        115                 120                 125             
Gly Tyr Lys Ser Ser Arg Arg Asn Glu Glu Asn Asp Lys Asp Gly Pro 
    130                 135                 140                 
Val Lys Ser Ser Ile Glu Glu Phe Arg Arg Gln Leu Gly Asp Lys Ser 
145                 150                 155                 160 
Val Gly Gln Phe Leu Ser Glu Leu His Gln Glu Asn Lys Pro Ile Arg 
                165                 170                 175     
Ala Arg Arg Asp Gly Val Thr Asn Asn Asp Leu Phe His Tyr Phe Pro 
            180                 185                 190         
Asp Arg Glu Leu Ile Glu Lys Glu Phe Asn Asp Ile Trp Gln Lys Gln 
        195                 200                 205             
Gln Gln Ile Arg Ser Gln Gln Pro Asp Lys Asn Lys Gln Ile Leu Ser 
    210                 215                 220                 
Asp Ile Leu Thr Asn Glu Asn Lys Gln Thr Leu Phe Glu Val Ile Phe 
225                 230                 235                 240 
His Gln Arg Ser Leu Lys Pro Pro Ile Ile Gly Asn Cys Gln Phe Phe 
                245                 250                 255     
Pro Thr Lys Lys Arg Ile Ala Lys Ala Leu Pro Ser Phe Gln Arg Phe 
            260                 265                 270         
Arg Ile Leu Gln Asp Ile Asn Asn Leu Glu Tyr Phe Asp Glu Asn Glu 
        275                 280                 285             
Trp His Pro Leu Pro Ser Ser Ile Arg Asp Trp Ala Leu His Ile Leu 
    290                 295                 300                 
Phe Ala Gly Gly Asn Leu Thr Phe Lys Lys Leu Asn Ser Gln Met Lys 
305                 310                 315                 320 
Lys Asp Gly Glu Ile Asn Glu Ser Ala Phe Phe Asn Leu Glu Asp Glu 
                325                 330                 335     
Lys Arg Asn Asp Ile Lys Gly Asp Phe Thr Thr Lys Thr Met Lys Glu 
            340                 345                 350         
Ile Ile Pro Ala Leu Trp Asp Asn Trp Asp Leu His Lys Gln Asp Ser 
        355                 360                 365             
Met Ile Leu Leu Leu Ile Gly Asp Lys Ser Ile Asp Glu Asp Lys Met 
    370                 375                 380                 
Leu Asp Glu Asp Lys Met Leu Asp Glu Leu Ser Ser His Tyr Asn Leu 
385                 390                 395                 400 
Ser Glu Glu Glu Ala Gln Glu Cys Ala Asn Ala Asn Ile Asp Asn Arg 
                405                 410                 415     
Gln Gly Val Ser Gly Arg Ala Ser Leu Ser Leu Glu Ala Ile Ser Ile 
            420                 425                 430         
Leu Ile Pro Tyr Leu Glu Lys Gly Asn His Tyr Asp Lys Ala Leu Ala 
        435                 440                 445             
Glu Ser Gly Ile Glu Lys Asp Lys Ser Ser Lys His Asp Asp His Phe 
    450                 455                 460                 
Tyr Met Gln Pro Tyr Pro Glu Ile Leu Gly Gln Trp Cys Leu Pro Arg 
465                 470                 475                 480 
Lys Ser Glu Asp Glu Ser Asn Lys Glu Leu Trp Arg Ile Pro Asn Pro 
                485                 490                 495     
Thr Val His Val Ala Leu Asn Gln Leu Arg Ala Val Val Asn Asp Cys 
            500                 505                 510         
Ile Arg Ile Asn Gly Gly Glu Lys Pro Ser Gln Val Val Val Glu Leu 
        515                 520                 525             
Ala Arg Asp Leu Pro Ile Gly Ile Ala Thr Arg Gln Glu Ile Arg Lys 
    530                 535                 540                 
Lys Gln Ser Glu Asn Gln Arg Ala Arg Thr Gln Arg Arg Asn Lys Ile 
545                 550                 555                 560 
Glu Glu Leu Gly Glu Arg Ala Ser Ala Lys Ser Met Leu Arg Met Gln 
                565                 570                 575     
Leu Trp Glu Glu Ser Ser Pro Lys Asn Ala Asn Asn His Cys Cys Leu 
            580                 585                 590         
Tyr Cys Gly Lys Gln Ile Gly Cys Ala Ala Ala Val Ser Ser Pro Asp 
        595                 600                 605             
Phe Glu Ile Asp His Ile Leu Pro Phe Ser Lys Thr Leu Asp Asp Ser 
    610                 615                 620                 
Ala Ala Asn Lys Thr Leu Cys Cys Val Gln Cys Asn Arg Glu Lys Gly 
625                 630                 635                 640 
Asn Lys Thr Pro Tyr Glu Ala Trp Gly Ser Asn Glu Lys Arg Tyr Asp 
                645                 650                 655     
Glu Ile Lys Thr Arg Ala Ser Ala Leu Ser Pro Lys Lys Arg Arg Arg 
            660                 665                 670         
Phe Leu Pro Asp Ala Met Lys His Phe Asp Gly Asp Asn Asp Phe Leu 
        675                 680                 685             
Ala Arg Gln Leu Thr Asp Thr Gln Tyr Ile Ala Lys Val Thr Lys Arg 
    690                 695                 700                 
Tyr Phe Glu Ser Ile Ile Lys Pro Asn Asp Val Tyr Val Ile Pro Gly 
705                 710                 715                 720 
Arg Leu Thr Glu Ile Leu Arg Arg Lys Trp Gly Leu Asn Asn Ile Leu 
                725                 730                 735     
Asn Asp Asp Gly His Lys Asn Arg Asp Asp His Arg His His Ala Val 
            740                 745                 750         
Asp Ala Ala Val Ile Gly Ala Thr Thr Arg Ser Thr Leu Gln Lys Phe 
        755                 760                 765             
Ala Thr Glu Ala Gly Lys Asp Asn Ser Asp Leu Pro Asn Val Ser Ile 
    770                 775                 780                 
Thr Ala Pro Met Lys Val Phe Arg Glu Lys Val Glu Lys Thr Val Lys 
785                 790                 795                 800 
Asn Ile Val Val Ser His Lys Pro Asp Arg Phe Glu Ser Gly Ala Leu 
                805                 810                 815     
His Asn Asp Thr Ala Tyr Gly Leu Pro Ala Asp Tyr Lys Glu Gly Ala 
            820                 825                 830         
Gly Ala Gln Phe Val Arg His Arg Ile Met Leu Ser Asp Ile Thr Thr 
        835                 840                 845             
Asn Phe Ser Gln Lys Val Val Asn Ser Phe Leu Arg Glu Glu Leu Lys 
    850                 855                 860                 
Thr Leu Cys Asp Tyr Ala Thr Asp Lys Lys Ser Leu Asp Gln Glu Leu 
865                 870                 875                 880 
Lys Arg Tyr Gly Glu Lys Asn Asn Ile Arg Arg Val Leu Ile Glu Glu 
                885                 890                 895     
Lys Leu Ser Val Ile Ala Val Cys Asp Lys Asp Gly Lys Pro Tyr Lys 
            900                 905                 910         
Gly Tyr Lys Ser Asp Ser Asn Trp Ala Tyr Glu Ile Phe Glu Lys Phe 
        915                 920                 925             
Pro Asn Gly Asn Trp Asp Gly Glu Ile Ile Ser Thr Phe Asn Ala Asn 
    930                 935                 940                 
Gln Glu Lys Phe Thr Pro Leu Trp Lys Glu Lys Asn Pro Lys Ala Lys 
945                 950                 955                 960 
Leu Ile Met Arg Leu His Lys Asn Asp Met Val Ala Leu Asp Asp Asn 
                965                 970                 975     
Gly Leu Arg Lys Ile Cys Tyr Val Lys Thr Leu Ser Ala Ser Lys Ile 
            980                 985                 990         
Ala Met Val Glu His Phe Asp Ala Thr Pro Gly Arg Asn Pro Pro Thr 
        995                 1000                1005            
Ile Ile Thr Lys Ser Pro Asn Glu Phe Arg Lys Ile Asn Gly Arg Lys 
    1010                1015                1020                
Ile His Ile Ser Pro Gly Gly Leu Val Arg Asp Thr Lys Lys Asp Gly 
1025                1030                1035                1040
Arg Ser Asn His 
                

<210> 3
<211> 120
<212> RNA
<213> Homoeodictya palmata


<400> 3
tataacaaac aaagaaatgc ggtttattta acattaacat gtgataaatt cgcaacaacc     60

tttagggcaa cttcggttgc cctattgctt ttaatggcga tataaatatt ttattaatcc    120


<210> 4
<211> 36
<212> DNA
<213> Homoeodictya palmata


<400> 4
gttgtgttat gaccgcattt ctttgtttgg tataat                               36


