                         SEQUENCE LISTING

<110>  Universit degli Studi di Pavia
 
<120>  Targeted single domain antibodies with catalytic activity 
       (T-CAN)"

<130>  @E0115946

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  single domain antibody having ASNase activity

<400>  1

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Thr Val Pro Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Thr Lys Thr Thr Asp Thr Tyr 
            20                  25                  30          


Asp Met Ser Trp Tyr Arg Arg Ala Pro Gly Lys Gly Arg Asp Phe Val 
        35                  40                  45              


Ser Gly Ile Asp Asn Asp Gly Thr Thr Thr Tyr Val Asp Ser Val Ala 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Gln Gly Asn Ala Lys Asn Thr Ala Tyr Leu 
65                  70                  75                  80  


Gln Met Asp Ser Leu Lys Pro Asp Asp Thr Ala Met Tyr Tyr Cys Lys 
                85                  90                  95      


Pro Ser Leu Ala Glu Val Asn Tyr Thr Gly Thr Ile Ile Pro Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     


<210>  2
<211>  427
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct

<400>  2
ccatgggaca ggtgcagctg gtggaaagcg gcggaggaac agtgcctgct ggcggctctc       60

tgagactgag ctgtgccgcc tctaccaaga ccaccgacac ctacgacatg agctggtaca      120

gacgggcccc tggcaagggc agagatttcg tgtccggcat cgacaacgac ggcaccacca      180

cctacgtgga ctctgtggcc ggcagattca ccatcagcca gggcaacgcc aagaacaccg      240

cctacctgca gatggacagc ctgaagcccg acgacaccgc catgtactac tgcaagccca      300

gcctggccga agtgaactat accggcacca tcatcccttg gggccagggc acacaagtga      360

ccgtgtccag cgagaacctg tattttcaag gccaccacca tcaccaccac tgatgatgag      420

cggccgc                                                                427


<210>  3
<211>  398
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  3

Met Ala Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro 
1               5                   10                  15      


Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro 
            20                  25                  30          


Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu 
        35                  40                  45              


Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val 
65                  70                  75                  80  


Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr 
                85                  90                  95      


Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Val Asp Ile Gln Met Thr Gln 
    130                 135                 140                 


Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser 
145                 150                 155                 160 


Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln 
                165                 170                 175     


Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu 
            180                 185                 190         


His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
        195                 200                 205             


Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr 
    210                 215                 220                 


Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr 
225                 230                 235                 240 


Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                245                 250                 255     


Gly Gly Gly Ser Gly Gln Leu Val Gln Leu Val Glu Ser Gly Gly Gly 
            260                 265                 270         


Thr Val Pro Ala Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Thr 
        275                 280                 285             


Lys Thr Thr Asp Thr Tyr Asp Met Ser Trp Tyr Arg Arg Ala Pro Gly 
    290                 295                 300                 


Lys Gly Arg Asp Phe Val Ser Gly Ile Asp Asn Asp Gly Thr Thr Thr 
305                 310                 315                 320 


Tyr Val Asp Ser Val Ala Gly Arg Phe Thr Ile Ser Gln Gly Asn Ala 
                325                 330                 335     


Lys Asn Thr Ala Tyr Leu Gln Met Asp Ser Leu Lys Pro Asp Asp Thr 
            340                 345                 350         


Ala Met Tyr Tyr Cys Lys Pro Ser Leu Ala Glu Val Asn Tyr Thr Gly 
        355                 360                 365             


Thr Ile Ile Pro Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Glu 
    370                 375                 380                 


Asn Leu Tyr Phe Gln Gly His His His His His His His His 
385                 390                 395             


<210>  4
<211>  1194
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct

<400>  4
atggccgagg tgaaactgca ggagtcagga cctggcctgg tggcgccctc acagagcctg       60

tccgtcacat gcactgtctc aggggtctca ttacccgact atggtgtaag ctggattcgc      120

cagcctccac gaaagggtct ggagtggctg ggagtaatat ggggtagtga aaccacatac      180

tataattcag ctctcaaatc cagactgacc atcatcaagg acaactccaa gagccaagtt      240

ttcttaaaaa tgaacagtct gcaaactgat gacacagcca tttactactg tgccaaacat      300

tattactacg gtggtagcta tgctatggac tactggggtc aaggaacctc agtcaccgtc      360

tcgagtggtg gaggcggttc aggcggaggt ggctctggcg gtggcggtag tgtcgacatc      420

cagatgacac agactacatc ctccctgtct gcctctctgg gagacagagt caccatcagt      480

tgcagggcaa gtcaggacat tagtaaatat ttaaattggt atcagcagaa accagatgga      540

actgttaaac tcctgatcta ccatacatca agattacact caggagtccc atcaaggttc      600

agtggcagtg ggtctggaac agattattct ctcaccatta gcaacctgga gcaagaagat      660

attgccactt acttttgcca acagggtaat acgcttccgt acacgttcgg aggggggact      720

aagcttgaaa taacaggtgg aggcggttca ggcggaggtg gctctggcgg tggtggctct      780

ggccaattgg tgcagctggt ggaaagcggc ggcggcaccg tgccggcggg cggcagcctg      840

cgcctgagct gcgcggcgag caccaaaacc accgatacct atgatatgag ctggtatcgc      900

cgcgcgccgg gcaaaggccg cgattttgtg agcggcattg ataacgatgg caccaccacc      960

tatgtggata gcgtggcggg ccgctttacc attagccagg gcaacgcgaa aaacaccgcg     1020

tatctgcaga tggatagcct gaaaccggat gataccgcga tgtattattg caaaccgagc     1080

ctggcggaag tgaactatac cggcaccatt attccgtggg gccagggcac ccaggtgacc     1140

gtgagcagcg aaaacctgta ttttcaggga catcatcatc atcatcatca ccat           1194


