                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
       Roche Diagnostics GmbH
       Roche Molecular Systems, Inc.
 
<120>  MUTANT DNA POLYMERASE(S) WITH IMPROVED STRAND DISPLACEMENT 
       ABILITY

<130>  P34971-WO-HS

<150>  62/730,908
<151>  2018-09-13

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  834
<212>  PRT
<213>  Thermus sp.


<220>
<221>  MISC_FEATURE
<222>  (1)..(834)
<223>  Thermus sp. Z05 DNA polymerase (Z05)

<400>  1

Met Lys Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Tyr Lys Ala Val Phe 
    50                  55                  60                  


Val Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu 
65                  70                  75                  80  


Ala Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln 
                85                  90                  95      


Leu Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Phe Thr Arg Leu 
            100                 105                 110         


Glu Val Pro Gly Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys 
        115                 120                 125             


Lys Ala Glu Arg Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg 
    130                 135                 140                 


Asp Leu Tyr Gln Leu Val Ser Asp Arg Val Ala Val Leu His Pro Glu 
145                 150                 155                 160 


Gly His Leu Ile Thr Pro Glu Trp Leu Trp Glu Lys Tyr Gly Leu Lys 
                165                 170                 175     


Pro Glu Gln Trp Val Asp Phe Arg Ala Leu Val Gly Asp Pro Ser Asp 
            180                 185                 190         


Asn Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu 
        195                 200                 205             


Leu Lys Glu Trp Gly Ser Leu Glu Asn Ile Leu Lys Asn Leu Asp Arg 
    210                 215                 220                 


Val Lys Pro Glu Ser Val Arg Glu Arg Ile Lys Ala His Leu Glu Asp 
225                 230                 235                 240 


Leu Lys Leu Ser Leu Glu Leu Ser Arg Val Arg Ser Asp Leu Pro Leu 
                245                 250                 255     


Glu Val Asp Phe Ala Arg Arg Arg Glu Pro Asp Arg Glu Gly Leu Arg 
            260                 265                 270         


Ala Phe Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             


Leu Leu Glu Ala Pro Ala Pro Leu Glu Glu Ala Pro Trp Pro Pro Pro 
    290                 295                 300                 


Glu Gly Ala Phe Val Gly Phe Val Leu Ser Arg Pro Glu Pro Met Trp 
305                 310                 315                 320 


Ala Glu Leu Lys Ala Leu Ala Ala Cys Lys Glu Gly Arg Val His Arg 
                325                 330                 335     


Ala Lys Asp Pro Leu Ala Gly Leu Lys Asp Leu Lys Glu Val Arg Gly 
            340                 345                 350         


Leu Leu Ala Lys Asp Leu Ala Val Leu Ala Leu Arg Glu Gly Leu Asp 
        355                 360                 365             


Leu Ala Pro Ser Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro 
    370                 375                 380                 


Ser Asn Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp 
385                 390                 395                 400 


Thr Glu Asp Ala Ala His Arg Ala Leu Leu Ala Glu Arg Leu Gln Gln 
                405                 410                 415     


Asn Leu Leu Glu Arg Leu Lys Gly Glu Glu Lys Leu Leu Trp Leu Tyr 
            420                 425                 430         


Gln Glu Val Glu Lys Pro Leu Ser Arg Val Leu Ala His Met Glu Ala 
        435                 440                 445             


Thr Gly Val Arg Leu Asp Val Ala Tyr Leu Lys Ala Leu Ser Leu Glu 
    450                 455                 460                 


Leu Ala Glu Glu Ile Arg Arg Leu Glu Glu Glu Val Phe Arg Leu Ala 
465                 470                 475                 480 


Gly His Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu 
                485                 490                 495     


Phe Asp Glu Leu Arg Leu Pro Ala Leu Gly Lys Thr Gln Lys Thr Gly 
            500                 505                 510         


Lys Arg Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His 
        515                 520                 525             


Pro Ile Val Glu Lys Ile Leu Gln His Arg Glu Leu Thr Lys Leu Lys 
    530                 535                 540                 


Asn Thr Tyr Val Asp Pro Leu Pro Gly Leu Val His Pro Arg Thr Gly 
545                 550                 555                 560 


Arg Leu His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu 
                565                 570                 575     


Ser Ser Ser Asp Pro Asn Leu Gln Asn Ile Pro Ile Arg Thr Pro Leu 
            580                 585                 590         


Gly Gln Arg Ile Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu 
        595                 600                 605             


Val Ala Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu 
    610                 615                 620                 


Ser Gly Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Lys Asp Ile 
625                 630                 635                 640 


His Thr Gln Thr Ala Ser Trp Met Phe Gly Val Ser Pro Glu Ala Val 
                645                 650                 655     


Asp Pro Leu Met Arg Arg Ala Ala Lys Thr Val Asn Phe Gly Val Leu 
            660                 665                 670         


Tyr Gly Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr 
        675                 680                 685             


Glu Glu Ala Val Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys 
    690                 695                 700                 


Val Arg Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Lys Arg Gly 
705                 710                 715                 720 


Tyr Val Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn 
                725                 730                 735     


Ala Arg Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn 
            740                 745                 750         


Met Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val 
        755                 760                 765             


Lys Leu Phe Pro His Leu Arg Glu Met Gly Ala Arg Met Leu Leu Gln 
    770                 775                 780                 


Val His Asp Glu Leu Leu Leu Glu Ala Pro Gln Ala Arg Ala Glu Glu 
785                 790                 795                 800 


Val Ala Ala Leu Ala Lys Glu Ala Met Glu Lys Ala Tyr Pro Leu Ala 
                805                 810                 815     


Val Pro Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala 
            820                 825                 830         


Lys Gly 
        


<210>  2
<211>  832
<212>  PRT
<213>  Thermus aquaticus


<220>
<221>  MISC_FEATURE
<222>  (1)..(832)
<223>  Thermus aquaticus DNA polymerase (Taq)

<400>  2

Met Arg Gly Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe His Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Ala Val Ile Val 
    50                  55                  60                  


Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Gly Gly 
65                  70                  75                  80  


Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu 
                85                  90                  95      


Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Ala Arg Leu Glu 
            100                 105                 110         


Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Ser Leu Ala Lys Lys 
        115                 120                 125             


Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Lys Asp 
    130                 135                 140                 


Leu Tyr Gln Leu Leu Ser Asp Arg Ile His Val Leu His Pro Glu Gly 
145                 150                 155                 160 


Tyr Leu Ile Thr Pro Ala Trp Leu Trp Glu Lys Tyr Gly Leu Arg Pro 
                165                 170                 175     


Asp Gln Trp Ala Asp Tyr Arg Ala Leu Thr Gly Asp Glu Ser Asp Asn 
            180                 185                 190         


Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Arg Lys Leu Leu 
        195                 200                 205             


Glu Glu Trp Gly Ser Leu Glu Ala Leu Leu Lys Asn Leu Asp Arg Leu 
    210                 215                 220                 


Lys Pro Ala Ile Arg Glu Lys Ile Leu Ala His Met Asp Asp Leu Lys 
225                 230                 235                 240 


Leu Ser Trp Asp Leu Ala Lys Val Arg Thr Asp Leu Pro Leu Glu Val 
                245                 250                 255     


Asp Phe Ala Lys Arg Arg Glu Pro Asp Arg Glu Arg Leu Arg Ala Phe 
            260                 265                 270         


Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu 
        275                 280                 285             


Glu Ser Pro Lys Ala Leu Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly 
    290                 295                 300                 


Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp 
305                 310                 315                 320 


Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro 
                325                 330                 335     


Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu 
            340                 345                 350         


Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro 
        355                 360                 365             


Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn 
    370                 375                 380                 


Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu 
385                 390                 395                 400 


Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu 
                405                 410                 415     


Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu 
            420                 425                 430         


Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly 
        435                 440                 445             


Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala 
    450                 455                 460                 


Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His 
465                 470                 475                 480 


Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
                485                 490                 495     


Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg 
            500                 505                 510         


Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile 
        515                 520                 525             


Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr 
    530                 535                 540                 


Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu 
545                 550                 555                 560 


His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser 
                565                 570                 575     


Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
            580                 585                 590         


Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala 
        595                 600                 605             


Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
    610                 615                 620                 


Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr 
625                 630                 635                 640 


Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro 
                645                 650                 655     


Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly 
            660                 665                 670         


Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu 
        675                 680                 685             


Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
    690                 695                 700                 


Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val 
705                 710                 715                 720 


Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg 
                725                 730                 735     


Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro 
            740                 745                 750         


Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu 
        755                 760                 765             


Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His 
    770                 775                 780                 


Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala 
785                 790                 795                 800 


Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro 
                805                 810                 815     


Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830         


<210>  3
<211>  830
<212>  PRT
<213>  Thermus filiformis


<220>
<221>  MISC_FEATURE
<222>  (1)..(830)
<223>  Thermus filiformis DNA polymerase (Tfi)

<400>  3

Met Leu Pro Leu Leu Glu Pro Lys Gly Arg Val Leu Leu Val Asp Gly 
1               5                   10                  15      


His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly Leu Thr Thr 
            20                  25                  30          


Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala Lys Ser Leu 
        35                  40                  45              


Leu Lys Ala Leu Lys Glu Asp Gly Glu Val Ala Ile Val Val Phe Asp 
    50                  55                  60                  


Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu Ala Tyr Lys Ala 
65                  70                  75                  80  


Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu Ala Leu Ile 
                85                  90                  95      


Lys Glu Leu Val Asp Leu Leu Gly Leu Val Arg Leu Glu Val Pro Gly 
            100                 105                 110         


Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Arg Lys Ala Glu Arg 
        115                 120                 125             


Glu Gly Tyr Glu Val Arg Ile Leu Ser Ala Asp Arg Asp Leu Tyr Gln 
    130                 135                 140                 


Leu Leu Ser Asp Arg Ile His Leu Leu His Pro Glu Gly Glu Val Leu 
145                 150                 155                 160 


Thr Pro Gly Trp Leu Gln Glu Arg Tyr Gly Leu Ser Pro Glu Arg Trp 
                165                 170                 175     


Val Glu Tyr Arg Ala Leu Val Gly Asp Pro Ser Asp Asn Leu Pro Gly 
            180                 185                 190         


Val Pro Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu Leu Lys Glu Trp 
        195                 200                 205             


Gly Ser Leu Glu Ala Ile Leu Lys Asn Leu Asp Gln Val Lys Pro Glu 
    210                 215                 220                 


Arg Val Trp Glu Ala Ile Arg Asn Asn Leu Asp Lys Leu Gln Met Ser 
225                 230                 235                 240 


Leu Glu Leu Ser Arg Leu Arg Thr Asp Leu Pro Leu Glu Val Asp Phe 
                245                 250                 255     


Ala Lys Arg Arg Glu Pro Thr Gly Lys Gly Leu Lys Ala Phe Leu Glu 
            260                 265                 270         


Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu Glu Ala 
        275                 280                 285             


Pro Lys Glu Ala Glu Glu Ala Pro Trp Pro Pro Pro Gly Gly Ala Phe 
    290                 295                 300                 


Leu Gly Phe Leu Leu Ser Arg Pro Glu Pro Met Trp Ala Glu Leu Leu 
305                 310                 315                 320 


Ala Leu Ala Gly Ala Lys Glu Gly Arg Val His Arg Ala Glu Asp Pro 
                325                 330                 335     


Val Gly Ala Leu Lys Asp Leu Lys Glu Ile Arg Gly Leu Leu Ala Lys 
            340                 345                 350         


Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Arg Glu Ile Pro Pro Gly 
        355                 360                 365             


Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Gly Asn Thr Asn 
    370                 375                 380                 


Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Lys Glu Asp Ala 
385                 390                 395                 400 


Ala Ala Arg Ala Leu Leu Ser Glu Arg Leu Trp Gln Ala Leu Tyr Pro 
                405                 410                 415     


Arg Val Ala Glu Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu 
            420                 425                 430         


Arg Pro Leu Ala Gln Val Leu Ala His Met Glu Ala Thr Gly Val Arg 
        435                 440                 445             


Leu Asp Val Pro Tyr Leu Glu Ala Leu Ser Gln Glu Val Ala Phe Glu 
    450                 455                 460                 


Leu Glu Arg Leu Glu Ala Glu Val His Arg Leu Ala Gly His Pro Phe 
465                 470                 475                 480 


Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu 
                485                 490                 495     


Gly Leu Pro Pro Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr 
            500                 505                 510         


Ser Ala Ala Val Leu Glu Leu Leu Arg Glu Ala His Pro Ile Val Gly 
        515                 520                 525             


Arg Ile Leu Glu Tyr Arg Glu Leu Met Lys Leu Lys Ser Thr Tyr Ile 
    530                 535                 540                 


Asp Pro Leu Pro Arg Leu Val His Pro Lys Thr Gly Arg Leu His Thr 
545                 550                 555                 560 


Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp 
                565                 570                 575     


Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile 
            580                 585                 590         


Arg Lys Ala Phe Ile Ala Glu Glu Gly His Leu Leu Val Ala Leu Asp 
        595                 600                 605             


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 
    610                 615                 620                 


Asn Leu Ile Arg Val Phe Arg Glu Gly Lys Asp Ile His Thr Glu Thr 
625                 630                 635                 640 


Ala Ala Trp Met Phe Gly Val Pro Pro Glu Gly Val Asp Gly Ala Met 
                645                 650                 655     


Arg Arg Ala Ala Lys Thr Val Asn Phe Gly Val Leu Tyr Gly Met Ser 
            660                 665                 670         


Ala His Arg Leu Ser Gln Glu Leu Ser Ile Pro Tyr Glu Glu Ala Ala 
        675                 680                 685             


Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp 
    690                 695                 700                 


Ile Ala Lys Thr Leu Glu Glu Gly Arg Lys Lys Gly Tyr Val Glu Thr 
705                 710                 715                 720 


Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn Ala Arg Val Lys 
                725                 730                 735     


Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln 
            740                 745                 750         


Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro 
        755                 760                 765             


Arg Leu Arg Pro Leu Gly Val Arg Ile Leu Leu Gln Val His Asp Glu 
    770                 775                 780                 


Leu Val Leu Glu Ala Pro Lys Ala Arg Ala Glu Glu Ala Ala Gln Leu 
785                 790                 795                 800 


Ala Lys Glu Thr Met Glu Gly Val Tyr Pro Leu Ser Val Pro Leu Glu 
                805                 810                 815     


Val Glu Val Gly Met Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830 


<210>  4
<211>  831
<212>  PRT
<213>  Thermus flavus


<220>
<221>  MISC_FEATURE
<222>  (1)..(831)
<223>  Thermus flavus DNA polymerase (Tfl)

<400>  4

Met Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu Val 
1               5                   10                  15      


Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly Leu 
            20                  25                  30          


Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala Lys 
        35                  40                  45              


Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Val Val Val Val Val 
    50                  55                  60                  


Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu Ala Tyr 
65                  70                  75                  80  


Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu Ala 
                85                  90                  95      


Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Val Arg Leu Glu Val 
            100                 105                 110         


Pro Gly Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys Arg Ala 
        115                 120                 125             


Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg Asp Leu 
    130                 135                 140                 


Tyr Gln Leu Leu Ser Glu Arg Ile Ala Ile Leu His Pro Glu Gly Tyr 
145                 150                 155                 160 


Leu Ile Thr Pro Ala Trp Leu Tyr Glu Lys Tyr Gly Leu Arg Pro Glu 
                165                 170                 175     


Gln Trp Val Asp Tyr Arg Ala Leu Ala Gly Asp Pro Ser Asp Asn Ile 
            180                 185                 190         


Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Gln Arg Leu Ile Arg 
        195                 200                 205             


Glu Trp Gly Ser Leu Glu Asn Leu Phe Gln His Leu Asp Gln Val Lys 
    210                 215                 220                 


Pro Ser Leu Arg Glu Lys Leu Gln Ala Gly Met Glu Ala Leu Ala Leu 
225                 230                 235                 240 


Ser Arg Lys Leu Ser Gln Val His Thr Asp Leu Pro Leu Glu Val Asp 
                245                 250                 255     


Phe Gly Arg Arg Arg Thr Pro Asn Leu Glu Gly Leu Arg Ala Phe Leu 
            260                 265                 270         


Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu Glu 
        275                 280                 285             


Gly Pro Lys Ala Ala Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala 
    290                 295                 300                 


Phe Leu Gly Phe Ser Phe Ser Arg Pro Glu Pro Met Trp Ala Glu Leu 
305                 310                 315                 320 


Leu Ala Leu Ala Gly Ala Trp Glu Gly Arg Leu His Arg Ala Gln Asp 
                325                 330                 335     


Pro Leu Arg Gly Leu Arg Asp Leu Lys Gly Val Arg Gly Ile Leu Ala 
            340                 345                 350         


Lys Asp Leu Ala Val Leu Ala Leu Arg Glu Gly Leu Asp Leu Phe Pro 
        355                 360                 365             


Glu Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr 
    370                 375                 380                 


Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Asp 
385                 390                 395                 400 


Ala Gly Glu Arg Ala Leu Leu Ala Glu Arg Leu Phe Gln Thr Leu Lys 
                405                 410                 415     


Glu Arg Leu Lys Gly Glu Glu Arg Leu Leu Trp Leu Tyr Glu Glu Val 
            420                 425                 430         


Glu Lys Pro Leu Ser Arg Val Leu Ala Arg Met Glu Ala Thr Gly Val 
        435                 440                 445             


Arg Leu Asp Val Ala Tyr Leu Gln Ala Leu Ser Leu Glu Val Glu Ala 
    450                 455                 460                 


Glu Val Arg Gln Leu Glu Glu Glu Val Phe Arg Leu Ala Gly His Pro 
465                 470                 475                 480 


Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu 
                485                 490                 495     


Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser 
            500                 505                 510         


Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val 
        515                 520                 525             


Asp Arg Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Asn Thr Tyr 
    530                 535                 540                 


Ile Asp Pro Leu Pro Ala Leu Val His Pro Lys Thr Gly Arg Leu His 
545                 550                 555                 560 


Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser 
                565                 570                 575     


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg 
            580                 585                 590         


Ile Arg Arg Ala Phe Val Ala Glu Glu Gly Trp Val Leu Val Val Leu 
        595                 600                 605             


Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp 
    610                 615                 620                 


Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr Gln 
625                 630                 635                 640 


Thr Ala Ser Trp Met Phe Gly Val Ser Pro Glu Gly Val Asp Pro Leu 
                645                 650                 655     


Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met 
            660                 665                 670         


Ser Ala His Arg Leu Ser Gly Glu Leu Ser Ile Pro Tyr Glu Glu Ala 
        675                 680                 685             


Val Ala Phe Ile Glu Arg Tyr Phe Gln Ser Tyr Pro Lys Val Arg Ala 
    690                 695                 700                 


Trp Ile Glu Gly Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu 
705                 710                 715                 720 


Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn Ala Arg Val 
                725                 730                 735     


Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val 
            740                 745                 750         


Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Arg Leu Phe 
        755                 760                 765             


Pro Arg Leu Gln Glu Leu Gly Ala Arg Met Leu Leu Gln Val His Asp 
    770                 775                 780                 


Glu Leu Val Leu Glu Ala Pro Lys Asp Arg Ala Glu Arg Val Ala Ala 
785                 790                 795                 800 


Leu Ala Lys Glu Val Met Glu Gly Val Trp Pro Leu Gln Val Pro Leu 
                805                 810                 815     


Glu Val Glu Val Gly Leu Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830     


<210>  5
<211>  830
<212>  PRT
<213>  Thermus sp.


<220>
<221>  MISC_FEATURE
<222>  (1)..(830)
<223>  Thermus sp. sps17 DNA polymerase (Sps17)

<400>  5

Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu Val Asp Gly 
1               5                   10                  15      


His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly Leu Thr Thr 
            20                  25                  30          


Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala Lys Ser Leu 
        35                  40                  45              


Leu Lys Ala Leu Lys Glu Asp Gly Glu Val Ala Ile Val Val Phe Asp 
    50                  55                  60                  


Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu Ala Tyr Lys Ala 
65                  70                  75                  80  


Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu Ala Leu Ile 
                85                  90                  95      


Lys Glu Leu Val Asp Leu Leu Gly Leu Val Arg Leu Glu Val Pro Gly 
            100                 105                 110         


Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys Lys Ala Glu Arg 
        115                 120                 125             


Glu Gly Tyr Glu Val Arg Ile Leu Ser Ala Asp Arg Asp Leu Tyr Gln 
    130                 135                 140                 


Leu Leu Ser Asp Arg Ile His Leu Leu His Pro Glu Gly Glu Val Leu 
145                 150                 155                 160 


Thr Pro Gly Trp Leu Gln Glu Arg Tyr Gly Leu Ser Pro Glu Arg Trp 
                165                 170                 175     


Val Glu Tyr Arg Ala Leu Val Gly Asp Pro Ser Asp Asn Leu Pro Gly 
            180                 185                 190         


Val Pro Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu Leu Lys Glu Trp 
        195                 200                 205             


Gly Ser Leu Glu Ala Ile Leu Lys Asn Leu Asp Gln Val Lys Pro Glu 
    210                 215                 220                 


Arg Val Arg Glu Ala Ile Arg Asn Asn Leu Asp Lys Leu Gln Met Ser 
225                 230                 235                 240 


Leu Glu Leu Ser Arg Leu Arg Thr Asp Leu Pro Leu Glu Val Asp Phe 
                245                 250                 255     


Ala Lys Arg Arg Glu Pro Asp Trp Glu Gly Leu Lys Ala Phe Leu Glu 
            260                 265                 270         


Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu Glu Ala 
        275                 280                 285             


Pro Lys Glu Ala Glu Glu Ala Pro Trp Pro Pro Pro Gly Gly Ala Phe 
    290                 295                 300                 


Leu Gly Phe Leu Leu Ser Arg Pro Glu Pro Met Trp Ala Glu Leu Leu 
305                 310                 315                 320 


Ala Leu Ala Gly Ala Lys Glu Gly Arg Val His Arg Ala Glu Asp Pro 
                325                 330                 335     


Val Gly Ala Leu Lys Asp Leu Lys Glu Ile Arg Gly Leu Leu Ala Lys 
            340                 345                 350         


Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Arg Glu Ile Pro Pro Gly 
        355                 360                 365             


Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Gly Asn Thr Asn 
    370                 375                 380                 


Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Lys Glu Asp Ala 
385                 390                 395                 400 


Ala Ala Arg Ala Leu Leu Ser Glu Arg Leu Trp Gln Ala Leu Tyr Pro 
                405                 410                 415     


Arg Val Ala Glu Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu 
            420                 425                 430         


Arg Pro Leu Ala Gln Val Leu Ala His Met Glu Ala Thr Gly Val Arg 
        435                 440                 445             


Leu Asp Val Pro Tyr Leu Glu Ala Leu Ser Gln Glu Val Ala Phe Glu 
    450                 455                 460                 


Leu Glu Arg Leu Glu Ala Glu Val His Arg Leu Ala Gly His Pro Phe 
465                 470                 475                 480 


Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu 
                485                 490                 495     


Gly Leu Pro Pro Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr 
            500                 505                 510         


Ser Ala Ala Val Leu Glu Leu Leu Arg Glu Ala His Pro Ile Val Gly 
        515                 520                 525             


Arg Ile Leu Glu Tyr Arg Glu Leu Met Lys Leu Lys Ser Thr Tyr Ile 
    530                 535                 540                 


Asp Pro Leu Pro Arg Leu Val His Pro Lys Thr Gly Arg Leu His Thr 
545                 550                 555                 560 


Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp 
                565                 570                 575     


Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile 
            580                 585                 590         


Arg Lys Ala Phe Ile Ala Glu Glu Gly His Leu Leu Val Ala Leu Asp 
        595                 600                 605             


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 
    610                 615                 620                 


Asn Leu Ile Arg Val Phe Arg Glu Gly Lys Asp Ile His Thr Glu Thr 
625                 630                 635                 640 


Ala Ala Trp Met Phe Gly Val Pro Pro Glu Gly Val Asp Gly Ala Met 
                645                 650                 655     


Arg Arg Ala Ala Lys Thr Val Asn Phe Gly Val Leu Tyr Gly Met Ser 
            660                 665                 670         


Ala His Arg Leu Ser Gln Glu Leu Ser Ile Pro Tyr Glu Glu Ala Ala 
        675                 680                 685             


Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp 
    690                 695                 700                 


Ile Ala Lys Thr Leu Glu Glu Gly Arg Lys Lys Gly Tyr Val Glu Thr 
705                 710                 715                 720 


Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn Ala Arg Val Lys 
                725                 730                 735     


Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln 
            740                 745                 750         


Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro 
        755                 760                 765             


Arg Leu Arg Pro Leu Gly Val Arg Ile Leu Leu Gln Val His Asp Glu 
    770                 775                 780                 


Leu Val Leu Glu Ala Pro Lys Ala Arg Ala Glu Glu Ala Ala Gln Leu 
785                 790                 795                 800 


Ala Lys Glu Thr Met Glu Gly Val Tyr Pro Leu Ser Val Pro Leu Glu 
                805                 810                 815     


Val Glu Val Gly Met Gly Glu Asp Trp Leu Ser Ala Lys Ala 
            820                 825                 830 


<210>  6
<211>  834
<212>  PRT
<213>  Thermus thermophilus


<220>
<221>  MISC_FEATURE
<222>  (1)..(834)
<223>  Thermus thermophilus DNA polymerase (Tth)

<400>  6

Met Glu Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Tyr Lys Ala Val Phe 
    50                  55                  60                  


Val Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu 
65                  70                  75                  80  


Ala Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln 
                85                  90                  95      


Leu Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Phe Thr Arg Leu 
            100                 105                 110         


Glu Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys 
        115                 120                 125             


Lys Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg 
    130                 135                 140                 


Asp Leu Tyr Gln Leu Val Ser Asp Arg Val Ala Val Leu His Pro Glu 
145                 150                 155                 160 


Gly His Leu Ile Thr Pro Glu Trp Leu Trp Glu Lys Tyr Gly Leu Arg 
                165                 170                 175     


Pro Glu Gln Trp Val Asp Phe Arg Ala Leu Val Gly Asp Pro Ser Asp 
            180                 185                 190         


Asn Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu 
        195                 200                 205             


Leu Lys Glu Trp Gly Ser Leu Glu Asn Leu Leu Lys Asn Leu Asp Arg 
    210                 215                 220                 


Val Lys Pro Glu Asn Val Arg Glu Lys Ile Lys Ala His Leu Glu Asp 
225                 230                 235                 240 


Leu Arg Leu Ser Leu Glu Leu Ser Arg Val Arg Thr Asp Leu Pro Leu 
                245                 250                 255     


Glu Val Asp Leu Ala Gln Gly Arg Glu Pro Asp Arg Glu Gly Leu Arg 
            260                 265                 270         


Ala Phe Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             


Leu Leu Glu Ala Pro Ala Pro Leu Glu Glu Ala Pro Trp Pro Pro Pro 
    290                 295                 300                 


Glu Gly Ala Phe Val Gly Phe Val Leu Ser Arg Pro Glu Pro Met Trp 
305                 310                 315                 320 


Ala Glu Leu Lys Ala Leu Ala Ala Cys Arg Asp Gly Arg Val His Arg 
                325                 330                 335     


Ala Ala Asp Pro Leu Ala Gly Leu Lys Asp Leu Lys Glu Val Arg Gly 
            340                 345                 350         


Leu Leu Ala Lys Asp Leu Ala Val Leu Ala Ser Arg Glu Gly Leu Asp 
        355                 360                 365             


Leu Val Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro 
    370                 375                 380                 


Ser Asn Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp 
385                 390                 395                 400 


Thr Glu Asp Ala Ala His Arg Ala Leu Leu Ser Glu Arg Leu His Arg 
                405                 410                 415     


Asn Leu Leu Lys Arg Leu Glu Gly Glu Glu Lys Leu Leu Trp Leu Tyr 
            420                 425                 430         


His Glu Val Glu Lys Pro Leu Ser Arg Val Leu Ala His Met Glu Ala 
        435                 440                 445             


Thr Gly Val Arg Arg Asp Val Ala Tyr Leu Gln Ala Leu Ser Leu Glu 
    450                 455                 460                 


Leu Ala Glu Glu Ile Arg Arg Leu Glu Glu Glu Val Phe Arg Leu Ala 
465                 470                 475                 480 


Gly His Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu 
                485                 490                 495     


Phe Asp Glu Leu Arg Leu Pro Ala Leu Gly Lys Thr Gln Lys Thr Gly 
            500                 505                 510         


Lys Arg Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His 
        515                 520                 525             


Pro Ile Val Glu Lys Ile Leu Gln His Arg Glu Leu Thr Lys Leu Lys 
    530                 535                 540                 


Asn Thr Tyr Val Asp Pro Leu Pro Ser Leu Val His Pro Arg Thr Gly 
545                 550                 555                 560 


Arg Leu His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu 
                565                 570                 575     


Ser Ser Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu 
            580                 585                 590         


Gly Gln Arg Ile Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu 
        595                 600                 605             


Val Ala Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu 
    610                 615                 620                 


Ser Gly Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Lys Asp Ile 
625                 630                 635                 640 


His Thr Gln Thr Ala Ser Trp Met Phe Gly Val Pro Pro Glu Ala Val 
                645                 650                 655     


Asp Pro Leu Met Arg Arg Ala Ala Lys Thr Val Asn Phe Gly Val Leu 
            660                 665                 670         


Tyr Gly Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr 
        675                 680                 685             


Glu Glu Ala Val Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys 
    690                 695                 700                 


Val Arg Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Lys Arg Gly 
705                 710                 715                 720 


Tyr Val Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn 
                725                 730                 735     


Ala Arg Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn 
            740                 745                 750         


Met Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val 
        755                 760                 765             


Lys Leu Phe Pro Arg Leu Arg Glu Met Gly Ala Arg Met Leu Leu Gln 
    770                 775                 780                 


Val His Asp Glu Leu Leu Leu Glu Ala Pro Gln Ala Arg Ala Glu Glu 
785                 790                 795                 800 


Val Ala Ala Leu Ala Lys Glu Ala Met Glu Lys Ala Tyr Pro Leu Ala 
                805                 810                 815     


Val Pro Leu Glu Val Glu Val Gly Met Gly Glu Asp Trp Leu Ser Ala 
            820                 825                 830         


Lys Gly 
        


<210>  7
<211>  834
<212>  PRT
<213>  Thermus caldophilus


<220>
<221>  MISC_FEATURE
<222>  (1)..(834)
<223>  Thermus caldophilus DNA polymerase (Tca)

<400>  7

Met Glu Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Tyr Lys Ala Val Phe 
    50                  55                  60                  


Val Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu 
65                  70                  75                  80  


Ala Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln 
                85                  90                  95      


Leu Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Phe Thr Arg Leu 
            100                 105                 110         


Glu Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys 
        115                 120                 125             


Asn Pro Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg 
    130                 135                 140                 


Asp Leu Asp Gln Leu Val Ser Asp Arg Val Ala Val Leu His Pro Glu 
145                 150                 155                 160 


Gly His Leu Ile Thr Pro Glu Trp Leu Trp Gln Lys Tyr Gly Leu Lys 
                165                 170                 175     


Pro Glu Gln Trp Val Asp Phe Arg Ala Leu Val Gly Asp Pro Ser Asp 
            180                 185                 190         


Asn Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu 
        195                 200                 205             


Leu Lys Glu Trp Gly Ser Leu Glu Asn Leu Leu Lys Asn Leu Asp Arg 
    210                 215                 220                 


Val Lys Pro Glu Asn Val Arg Glu Lys Ile Lys Ala His Leu Glu Asp 
225                 230                 235                 240 


Leu Arg Leu Ser Leu Glu Leu Ser Arg Val Arg Thr Asp Leu Pro Leu 
                245                 250                 255     


Glu Val Asp Leu Ala Gln Gly Arg Glu Pro Asp Arg Glu Gly Leu Arg 
            260                 265                 270         


Ala Phe Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             


Leu Leu Glu Ala Pro Ala Pro Leu Glu Glu Ala Pro Trp Pro Pro Pro 
    290                 295                 300                 


Glu Gly Ala Phe Val Gly Phe Val Leu Ser Arg Pro Glu Pro Met Trp 
305                 310                 315                 320 


Ala Glu Leu Lys Ala Leu Ala Ala Cys Arg Asp Gly Arg Val His Arg 
                325                 330                 335     


Ala Ala Asp Pro Leu Ala Gly Leu Lys Asp Leu Lys Glu Val Arg Gly 
            340                 345                 350         


Leu Leu Ala Lys Asp Leu Ala Val Leu Ala Ser Arg Glu Gly Leu Asp 
        355                 360                 365             


Leu Val Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro 
    370                 375                 380                 


Ser Asn Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp 
385                 390                 395                 400 


Thr Glu Asp Ala Ala His Arg Ala Leu Leu Ser Glu Arg Leu His Arg 
                405                 410                 415     


Asn Leu Leu Lys Arg Leu Gln Gly Glu Glu Lys Leu Leu Trp Leu Tyr 
            420                 425                 430         


His Glu Val Glu Lys Pro Leu Ser Arg Val Leu Ala His Met Glu Ala 
        435                 440                 445             


Thr Gly Val Arg Leu Asp Val Ala Tyr Leu Gln Ala Leu Ser Leu Glu 
    450                 455                 460                 


Leu Ala Glu Glu Ile Arg Arg Leu Glu Glu Glu Val Phe Arg Leu Ala 
465                 470                 475                 480 


Gly His Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu 
                485                 490                 495     


Phe Asp Glu Leu Arg Leu Pro Ala Leu Gly Lys Thr Gln Lys Thr Gly 
            500                 505                 510         


Lys Arg Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His 
        515                 520                 525             


Pro Ile Val Glu Lys Ile Leu Gln His Arg Glu Leu Thr Lys Leu Lys 
    530                 535                 540                 


Asn Thr Tyr Val Asp Pro Leu Pro Ser Leu Val His Pro Asn Thr Gly 
545                 550                 555                 560 


Arg Leu His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu 
                565                 570                 575     


Ser Ser Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu 
            580                 585                 590         


Gly Gln Arg Ile Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu 
        595                 600                 605             


Val Ala Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu 
    610                 615                 620                 


Ser Gly Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Lys Asp Ile 
625                 630                 635                 640 


His Thr Gln Thr Ala Ser Trp Met Phe Gly Val Pro Pro Glu Ala Val 
                645                 650                 655     


Asp Pro Leu Met Arg Arg Ala Ala Lys Thr Val Asn Phe Gly Val Leu 
            660                 665                 670         


Tyr Gly Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr 
        675                 680                 685             


Glu Glu Ala Val Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys 
    690                 695                 700                 


Val Arg Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Lys Arg Gly 
705                 710                 715                 720 


Tyr Val Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn 
                725                 730                 735     


Ala Arg Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn 
            740                 745                 750         


Met Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val 
        755                 760                 765             


Lys Leu Phe Pro Arg Leu Arg Glu Met Gly Ala Arg Met Leu Leu Gln 
    770                 775                 780                 


Val His Asp Glu Leu Leu Leu Glu Ala Pro Gln Ala Gly Ala Glu Glu 
785                 790                 795                 800 


Val Ala Ala Leu Ala Lys Glu Ala Met Glu Lys Ala Tyr Pro Leu Ala 
                805                 810                 815     


Val Pro Leu Glu Val Glu Val Gly Met Gly Glu Asp Trp Leu Ser Ala 
            820                 825                 830         


Lys Gly 
        


<210>  8
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic DNA polymerase motif


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  Xaa = Leu or Ile

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa = Val, Leu, Ile or Phe

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa = Ala, Val, Ser or Gly

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa = Leu or Ala

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Xaa = any amino acid other than Ile, Lys, Asn, Gln or Thr

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  Xaa = Val, Ile or Leu

<220>
<221>  VARIANT
<222>  (17)..(17)
<223>  Xaa = Leu, Val or Ile

<220>
<221>  VARIANT
<222>  (18)..(18)
<223>  Xaa = Ser or Ala

<220>
<221>  VARIANT
<222>  (19)..(19)
<223>  Xaa = Gly, Lys, Asp or Glu

<400>  8

Xaa Xaa Xaa Xaa Asp Tyr Ser Gln Xaa Glu Leu Arg Xaa Leu Ala His 
1               5                   10                  15      


Xaa Xaa Xaa Asp 
            20  


<210>  9
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic DNA polymerase motif


<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa = Ala or Val

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Xaa = any amino acid other than Ile, Lys, Asn, Gln or Thr

<400>  9

Leu Val Xaa Leu Asp Tyr Ser Gln Xaa Glu Leu Arg Val Leu Ala His 
1               5                   10                  15      


Leu Ser Gly Asp 
            20  


<210>  10
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic DNA polymerase motif


<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Xaa = any amino acid other than Ile, Lys, Asn, Gln or Thr

<400>  10

Leu Val Ala Leu Asp Tyr Ser Gln Xaa Glu Leu Arg Val Leu Ala His 
1               5                   10                  15      


Leu Ser Gly Asp 
            20  


<210>  11
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic DNA polymerase motif

<400>  11

Leu Val Ala Leu Asp Tyr Ser Gln Met Glu Leu Arg Val Leu Ala His 
1               5                   10                  15      


Leu Ser Gly Asp 
            20  


<210>  12
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Thermus sp. Z05 DNA 
       polymerase (Z05)

<400>  12

Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu Val Ala Leu Asp 
1               5                   10                  15      


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 
            20                  25                  30          


Asn Leu Ile Arg Val Phe 
        35              


<210>  13
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Thermus aquaticus DNA 
       polymerase (Taq)

<400>  13

Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu Asp 
1               5                   10                  15      


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 
            20                  25                  30          


Asn Leu Ile Arg Val Phe 
        35              


<210>  14
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Thermus filiformus DNA 
       polymerase (Tfi)

<400>  14

Arg Lys Ala Phe Ile Ala Glu Glu Gly His Leu Leu Val Ala Leu Asp 
1               5                   10                  15      


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 
            20                  25                  30          


Asn Leu Ile Arg Val Phe 
        35              


<210>  15
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Thermus flavus DNA 
       polymerase (Tfl)

<400>  15

Arg Arg Ala Phe Val Ala Glu Glu Gly Trp Val Leu Val Val Leu Asp 
1               5                   10                  15      


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 
            20                  25                  30          


Asn Leu Ile Arg Val Phe 
        35              


<210>  16
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Thermus sp. sps17 DNA 
       polymerase (Sps17)

<400>  16

Arg Lys Ala Phe Ile Ala Glu Glu Gly His Leu Leu Val Ala Leu Asp 
1               5                   10                  15      


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 
            20                  25                  30          


Asn Leu Ile Arg Val Phe 
        35              


<210>  17
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Thermus thermophilus DNA 
       polymerase (Tth)

<400>  17

Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu Val Ala Leu Asp 
1               5                   10                  15      


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 
            20                  25                  30          


Asn Leu Ile Arg Val Phe 
        35              


<210>  18
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Thermus caldophilus DNA 
       polymerase (Tca)

<400>  18

Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu Val Ala Leu Asp 
1               5                   10                  15      


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 
            20                  25                  30          


Asn Leu Ile Arg Val Phe 
        35              


<210>  19
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Thermotoga maritima DNA 
       polymerase (Tma)

<400>  19

Arg Lys Ala Ile Val Pro Gln Asp Pro Asn Trp Trp Ile Val Ser Ala 
1               5                   10                  15      


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Leu Ala His Leu Ser Gly Asp 
            20                  25                  30          


Glu Asn Leu Leu Arg Ala Phe 
        35                  


<210>  20
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Thermotoga neopolitana DNA 
       polymerase (Tne)

<400>  20

Arg Lys Ala Ile Val Pro Gln Asp Pro Asp Trp Trp Ile Val Ser Ala 
1               5                   10                  15      


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Leu Ala His Leu Ser Gly Asp 
            20                  25                  30          


Glu Asn Leu Val Lys Ala Phe 
        35                  


<210>  21
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Thermosipho africanus DNA 
       polymerase (Taf)

<400>  21

Arg Lys Ala Val Arg Pro Gln Arg Gln Asp Trp Trp Ile Leu Gly Ala 
1               5                   10                  15      


Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Val Ser Lys Asp 
            20                  25                  30          


Glu Asn Leu Leu Lys Ala Phe 
        35                  


<210>  22
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic conserved DNA polymerase active site motif A

<400>  22

Asp Tyr Ser Gln Ile Glu Leu Arg 
1               5               


<210>  23
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Deinococcus radiodurans DNA
       polymerase (Dra)

<400>  23

Arg Lys Gly Phe Ile Ala Glu Asp Gly Phe Thr Leu Ile Ala Ala Asp 
1               5                   10                  15      


Tyr Ser Gln Ile Glu Leu Arg Leu Leu Ala His Ile Ala Asp Asp Pro 
            20                  25                  30          


Leu Met Gln Gln Ala Phe 
        35              


<210>  24
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Bacillus stearothermophilus
       DNA polymerase (Bst)

<400>  24

Arg Gln Ala Phe Val Pro Ser Glu Pro Asp Trp Leu Ile Phe Ala Ala 
1               5                   10                  15      


Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Ile Ala Glu Asp 
            20                  25                  30          


Asp Asn Leu Ile Glu Ala Phe 
        35                  


<210>  25
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region of Bacillus caldotenax DNA 
       polymerase (Bca)

<400>  25

Arg Gln Ala Phe Val Pro Ser Glu Ser Asp Trp Leu Ile Phe Ala Ala 
1               5                   10                  15      


Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Ile Ala Glu Asp 
            20                  25                  30          


Asp Asn Leu Met Glu Ala Phe 
        35                  


<210>  26
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase domain region native consensus motif


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  Xaa = Leu or Ile

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa = Val, Leu, Ile or Phe

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa = Ala, Val, Ser or Gly

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa = Leu or Ala

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  Xaa = Val, Ile or Leu

<220>
<221>  VARIANT
<222>  (17)..(17)
<223>  Xaa = Leu, Val or Ile

<220>
<221>  VARIANT
<222>  (18)..(18)
<223>  Xaa = Ser or Ala

<220>
<221>  VARIANT
<222>  (19)..(19)
<223>  Xaa = Gly, Lys, Asp or Glu

<400>  26

Xaa Xaa Xaa Xaa Asp Tyr Ser Gln Ile Glu Leu Arg Xaa Leu Ala His 
1               5                   10                  15      


Xaa Xaa Xaa Asp 
            20  


<210>  27
<211>  893
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic chimeric CS5 DNA polymerase derived from N-terminal 
       5'-nuclease domain of Thermus sp. Z05 and C-terminal 3'-5' 
       exonuclease and polymerase domains of Thermotoga maritima DNA 
       polymerases

<400>  27

Met Lys Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Tyr Lys Ala Val Phe 
    50                  55                  60                  


Val Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu 
65                  70                  75                  80  


Ala Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln 
                85                  90                  95      


Leu Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Phe Thr Arg Leu 
            100                 105                 110         


Glu Val Pro Gly Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys 
        115                 120                 125             


Lys Ala Glu Arg Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg 
    130                 135                 140                 


Asp Leu Tyr Gln Leu Val Ser Asp Arg Val Ala Val Leu His Pro Glu 
145                 150                 155                 160 


Gly His Leu Ile Thr Pro Glu Trp Leu Trp Glu Lys Tyr Gly Leu Lys 
                165                 170                 175     


Pro Glu Gln Trp Val Asp Phe Arg Ala Leu Val Gly Asp Pro Ser Asp 
            180                 185                 190         


Asn Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu 
        195                 200                 205             


Leu Lys Glu Trp Gly Ser Leu Glu Asn Ile Leu Lys Asn Leu Asp Arg 
    210                 215                 220                 


Val Lys Pro Glu Ser Val Arg Glu Arg Ile Lys Ala His Leu Glu Asp 
225                 230                 235                 240 


Leu Lys Leu Ser Leu Glu Leu Ser Arg Val Arg Ser Asp Leu Pro Leu 
                245                 250                 255     


Glu Val Asp Phe Ala Arg Arg Arg Glu Pro Asp Arg Glu Gly Leu Arg 
            260                 265                 270         


Ala Phe Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             


Leu Leu Glu Glu Ser Glu Pro Val Gly Tyr Arg Ile Val Lys Asp Leu 
    290                 295                 300                 


Val Glu Phe Glu Lys Leu Ile Glu Lys Leu Arg Glu Ser Pro Ser Phe 
305                 310                 315                 320 


Ala Ile Asp Leu Glu Thr Ser Ser Leu Asp Pro Phe Asp Cys Asp Ile 
                325                 330                 335     


Val Gly Ile Ser Val Ser Phe Lys Pro Lys Glu Ala Tyr Tyr Ile Pro 
            340                 345                 350         


Leu His His Arg Asn Ala Gln Asn Leu Asp Glu Lys Glu Val Leu Lys 
        355                 360                 365             


Lys Leu Lys Glu Ile Leu Glu Asp Pro Gly Ala Lys Ile Val Gly Gln 
    370                 375                 380                 


Asn Leu Lys Phe Asp Tyr Lys Val Leu Met Val Lys Gly Val Glu Pro 
385                 390                 395                 400 


Val Pro Pro Tyr Phe Asp Thr Met Ile Ala Ala Tyr Leu Leu Glu Pro 
                405                 410                 415     


Asn Glu Lys Lys Phe Asn Leu Asp Asp Leu Ala Leu Lys Phe Leu Gly 
            420                 425                 430         


Tyr Lys Met Thr Ser Tyr Gln Glu Leu Met Ser Phe Ser Phe Pro Leu 
        435                 440                 445             


Phe Gly Phe Ser Phe Ala Asp Val Pro Val Glu Lys Ala Ala Asn Tyr 
    450                 455                 460                 


Ser Cys Glu Asp Ala Asp Ile Thr Tyr Arg Leu Tyr Lys Thr Leu Ser 
465                 470                 475                 480 


Leu Lys Leu His Glu Ala Asp Leu Glu Asn Val Phe Tyr Lys Ile Glu 
                485                 490                 495     


Met Pro Leu Val Asn Val Leu Ala Arg Met Glu Leu Asn Gly Val Tyr 
            500                 505                 510         


Val Asp Thr Glu Phe Leu Lys Lys Leu Ser Glu Glu Tyr Gly Lys Lys 
        515                 520                 525             


Leu Glu Glu Leu Ala Glu Glu Ile Tyr Arg Ile Ala Gly Glu Pro Phe 
    530                 535                 540                 


Asn Ile Asn Ser Pro Lys Gln Val Ser Arg Ile Leu Phe Glu Lys Leu 
545                 550                 555                 560 


Gly Ile Lys Pro Arg Gly Lys Thr Thr Lys Thr Gly Asp Tyr Ser Thr 
                565                 570                 575     


Arg Ile Glu Val Leu Glu Glu Leu Ala Gly Glu His Glu Ile Ile Pro 
            580                 585                 590         


Leu Ile Leu Glu Tyr Arg Lys Ile Gln Lys Leu Lys Ser Thr Tyr Ile 
        595                 600                 605             


Asp Ala Leu Pro Lys Met Val Asn Pro Lys Thr Gly Arg Ile His Ala 
    610                 615                 620                 


Ser Phe Asn Gln Thr Gly Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp 
625                 630                 635                 640 


Pro Asn Leu Gln Asn Leu Pro Thr Lys Ser Glu Glu Gly Lys Glu Ile 
                645                 650                 655     


Arg Lys Ala Ile Val Pro Gln Asp Pro Asn Trp Trp Ile Val Ser Ala 
            660                 665                 670         


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Leu Ala His Leu Ser Gly Asp 
        675                 680                 685             


Glu Asn Leu Leu Arg Ala Phe Glu Glu Gly Ile Asp Val His Thr Leu 
    690                 695                 700                 


Thr Ala Ser Arg Ile Phe Asn Val Lys Pro Glu Glu Val Thr Glu Glu 
705                 710                 715                 720 


Met Arg Arg Ala Gly Lys Met Val Asn Phe Ser Ile Ile Tyr Gly Val 
                725                 730                 735     


Thr Pro Tyr Gly Leu Ser Val Arg Leu Gly Val Pro Val Lys Glu Ala 
            740                 745                 750         


Glu Lys Met Ile Val Asn Tyr Phe Val Leu Tyr Pro Lys Val Arg Asp 
        755                 760                 765             


Tyr Ile Gln Arg Val Val Ser Glu Ala Lys Glu Lys Gly Tyr Val Arg 
    770                 775                 780                 


Thr Leu Phe Gly Arg Lys Arg Asp Ile Pro Gln Leu Met Ala Arg Asp 
785                 790                 795                 800 


Arg Asn Thr Gln Ala Glu Gly Glu Arg Ile Ala Ile Asn Thr Pro Ile 
                805                 810                 815     


Gln Gly Thr Ala Ala Asp Ile Ile Lys Leu Ala Met Ile Glu Ile Asp 
            820                 825                 830         


Arg Glu Leu Lys Glu Arg Lys Met Arg Ser Lys Met Ile Ile Gln Val 
        835                 840                 845             


His Asp Glu Leu Val Phe Glu Val Pro Asn Glu Glu Lys Asp Ala Leu 
    850                 855                 860                 


Val Glu Leu Val Lys Asp Arg Met Thr Asn Val Val Lys Leu Ser Val 
865                 870                 875                 880 


Pro Leu Glu Val Asp Val Thr Ile Gly Lys Thr Trp Ser 
                885                 890             


<210>  28
<211>  893
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic chimeric CS6 DNA polymerase derived from N-terminal 
       5'-nuclease domain of Thermus sp. Z05 and C-terminal 3'-5' 
       exonuclease and polymerase domains of Thermotoga maritima DNA 
       polymerases

<400>  28

Met Lys Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Tyr Lys Ala Val Phe 
    50                  55                  60                  


Val Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu 
65                  70                  75                  80  


Ala Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln 
                85                  90                  95      


Leu Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Phe Thr Arg Leu 
            100                 105                 110         


Glu Val Pro Gly Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys 
        115                 120                 125             


Lys Ala Glu Arg Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg 
    130                 135                 140                 


Asp Leu Tyr Gln Leu Val Ser Asp Arg Val Ala Val Leu His Pro Glu 
145                 150                 155                 160 


Gly His Leu Ile Thr Pro Glu Trp Leu Trp Glu Lys Tyr Gly Leu Lys 
                165                 170                 175     


Pro Glu Gln Trp Val Asp Phe Arg Ala Leu Val Gly Asp Pro Ser Asp 
            180                 185                 190         


Asn Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu 
        195                 200                 205             


Leu Lys Glu Trp Gly Ser Leu Glu Asn Ile Leu Lys Asn Leu Asp Arg 
    210                 215                 220                 


Val Lys Pro Glu Ser Val Arg Glu Arg Ile Lys Ala His Leu Glu Asp 
225                 230                 235                 240 


Leu Lys Leu Ser Leu Glu Leu Ser Arg Val Arg Ser Asp Leu Pro Leu 
                245                 250                 255     


Glu Val Asp Phe Ala Arg Arg Arg Glu Pro Asp Arg Glu Gly Leu Arg 
            260                 265                 270         


Ala Phe Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             


Leu Leu Glu Glu Ser Glu Pro Val Gly Tyr Arg Ile Val Lys Asp Leu 
    290                 295                 300                 


Val Glu Phe Glu Lys Leu Ile Glu Lys Leu Arg Glu Ser Pro Ser Phe 
305                 310                 315                 320 


Ala Ile Ala Leu Ala Thr Ser Ser Leu Asp Pro Phe Asp Cys Asp Ile 
                325                 330                 335     


Val Gly Ile Ser Val Ser Phe Lys Pro Lys Glu Ala Tyr Tyr Ile Pro 
            340                 345                 350         


Leu His His Arg Asn Ala Gln Asn Leu Asp Glu Lys Glu Val Leu Lys 
        355                 360                 365             


Lys Leu Lys Glu Ile Leu Glu Asp Pro Gly Ala Lys Ile Val Gly Gln 
    370                 375                 380                 


Asn Leu Lys Phe Asp Tyr Lys Val Leu Met Val Lys Gly Val Glu Pro 
385                 390                 395                 400 


Val Pro Pro Tyr Phe Asp Thr Met Ile Ala Ala Tyr Leu Leu Glu Pro 
                405                 410                 415     


Asn Glu Lys Lys Phe Asn Leu Asp Asp Leu Ala Leu Lys Phe Leu Gly 
            420                 425                 430         


Tyr Lys Met Thr Ser Tyr Gln Glu Leu Met Ser Phe Ser Phe Pro Leu 
        435                 440                 445             


Phe Gly Phe Ser Phe Ala Asp Val Pro Val Glu Lys Ala Ala Asn Tyr 
    450                 455                 460                 


Ser Cys Glu Asp Ala Asp Ile Thr Tyr Arg Leu Tyr Lys Thr Leu Ser 
465                 470                 475                 480 


Leu Lys Leu His Glu Ala Asp Leu Glu Asn Val Phe Tyr Lys Ile Glu 
                485                 490                 495     


Met Pro Leu Val Asn Val Leu Ala Arg Met Glu Leu Asn Gly Val Tyr 
            500                 505                 510         


Val Asp Thr Glu Phe Leu Lys Lys Leu Ser Glu Glu Tyr Gly Lys Lys 
        515                 520                 525             


Leu Glu Glu Leu Ala Glu Glu Ile Tyr Arg Ile Ala Gly Glu Pro Phe 
    530                 535                 540                 


Asn Ile Asn Ser Pro Lys Gln Val Ser Arg Ile Leu Phe Glu Lys Leu 
545                 550                 555                 560 


Gly Ile Lys Pro Arg Gly Lys Thr Thr Lys Thr Gly Asp Tyr Ser Thr 
                565                 570                 575     


Arg Ile Glu Val Leu Glu Glu Leu Ala Gly Glu His Glu Ile Ile Pro 
            580                 585                 590         


Leu Ile Leu Glu Tyr Arg Lys Ile Gln Lys Leu Lys Ser Thr Tyr Ile 
        595                 600                 605             


Asp Ala Leu Pro Lys Met Val Asn Pro Lys Thr Gly Arg Ile His Ala 
    610                 615                 620                 


Ser Phe Asn Gln Thr Gly Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp 
625                 630                 635                 640 


Pro Asn Leu Gln Asn Leu Pro Thr Lys Ser Glu Glu Gly Lys Glu Ile 
                645                 650                 655     


Arg Lys Ala Ile Val Pro Gln Asp Pro Asn Trp Trp Ile Val Ser Ala 
            660                 665                 670         


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Leu Ala His Leu Ser Gly Asp 
        675                 680                 685             


Glu Asn Leu Leu Arg Ala Phe Glu Glu Gly Ile Asp Val His Thr Leu 
    690                 695                 700                 


Thr Ala Ser Arg Ile Phe Asn Val Lys Pro Glu Glu Val Thr Glu Glu 
705                 710                 715                 720 


Met Arg Arg Ala Gly Lys Met Val Asn Phe Ser Ile Ile Tyr Gly Val 
                725                 730                 735     


Thr Pro Tyr Gly Leu Ser Val Arg Leu Gly Val Pro Val Lys Glu Ala 
            740                 745                 750         


Glu Lys Met Ile Val Asn Tyr Phe Val Leu Tyr Pro Lys Val Arg Asp 
        755                 760                 765             


Tyr Ile Gln Arg Val Val Ser Glu Ala Lys Glu Lys Gly Tyr Val Arg 
    770                 775                 780                 


Thr Leu Phe Gly Arg Lys Arg Asp Ile Pro Gln Leu Met Ala Arg Asp 
785                 790                 795                 800 


Arg Asn Thr Gln Ala Glu Gly Glu Arg Ile Ala Ile Asn Thr Pro Ile 
                805                 810                 815     


Gln Gly Thr Ala Ala Asp Ile Ile Lys Leu Ala Met Ile Glu Ile Asp 
            820                 825                 830         


Arg Glu Leu Lys Glu Arg Lys Met Arg Ser Lys Met Ile Ile Gln Val 
        835                 840                 845             


His Asp Glu Leu Val Phe Glu Val Pro Asn Glu Glu Lys Asp Ala Leu 
    850                 855                 860                 


Val Glu Leu Val Lys Asp Arg Met Thr Asn Val Val Lys Leu Ser Val 
865                 870                 875                 880 


Pro Leu Glu Val Asp Val Thr Ile Gly Lys Thr Trp Ser 
                885                 890             


<210>  29
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic DNA polymerase motif


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  Xaa = Ala, Asp, Ser, Glu, Arg or Gln

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa = Trp or Tyr

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa = any amino acid other than Ile, Leu or Met

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa = Glu, Ala, Gln, Lys, Asn or Asp

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  Xaa = Lys, Gly, Arg, Gln, His or Asn

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  Xaa = Thr, Val, Met or Ile

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  Xaa = Leu, Val or Lys

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Xaa = Glu, Ser, Ala, Asp or Gln

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Xaa = Glu or Phe

<220>
<221>  VARIANT
<222>  (10)..(10)
<223>  Xaa = Gly or Ala

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  Xaa = Arg or Lys

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  Xaa = Lys, Arg, Glu, Thr or Gln

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  Xaa = Arg, Lys or His

<220>
<221>  VARIANT
<222>  (17)..(17)
<223>  Xaa = Glu, Arg or Thr

<400>  29

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly Tyr Val 
1               5                   10                  15      


Xaa Thr Leu 
            


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic error-prone (mutagenic) PCR amplification forward 
       primer

<400>  30
ctacctcctg gacccctcca a                                                 21


<210>  31
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic error-prone (mutagenic) PCR amplification reverse 
       primer

<400>  31
ataaccaact ggtagtggcg tgtaa                                             25


<210>  32
<211>  921
<212>  PRT
<213>  Deinococcus radiodurans


<220>
<221>  MISC_FEATURE
<222>  (1)..(921)
<223>  Deinococcus radiodurans DNA polymerase (Dra)

<400>  32

Met Ala Asp Ala Ser Pro Asp Pro Ser Lys Pro Asp Ala Leu Val Leu 
1               5                   10                  15      


Ile Asp Gly His Ala Leu Ala Phe Arg Ser Tyr Phe Ala Leu Pro Pro 
            20                  25                  30          


Leu Asn Asn Ser Lys Gly Glu Met Thr Asp Ala Ile Val Gly Phe Met 
        35                  40                  45              


Lys Leu Leu Leu Arg Leu Ala Arg Gln Lys Ser Asn Gln Val Ile Val 
    50                  55                  60                  


Val Phe Asp Pro Pro Val Lys Thr Leu Arg His Glu Gln Tyr Glu Gly 
65                  70                  75                  80  


Tyr Lys Ser Gly Arg Ala Gln Thr Pro Glu Asp Leu Arg Gly Gln Ile 
                85                  90                  95      


Asn Arg Ile Arg Ala Leu Val Asp Ala Leu Gly Phe Pro Arg Leu Glu 
            100                 105                 110         


Glu Pro Gly Tyr Glu Ala Asp Asp Val Ile Ala Ser Leu Thr Arg Met 
        115                 120                 125             


Ala Glu Gly Lys Gly Tyr Glu Val Arg Ile Val Thr Ser Asp Arg Asp 
    130                 135                 140                 


Ala Tyr Gln Leu Leu Asp Glu His Val Lys Val Ile Ala Asn Asp Phe 
145                 150                 155                 160 


Ser Leu Ile Gly Pro Ala Gln Val Glu Glu Lys Tyr Gly Val Thr Val 
                165                 170                 175     


Arg Gln Trp Val Asp Tyr Arg Ala Leu Thr Gly Asp Ala Ser Asp Asn 
            180                 185                 190         


Ile Pro Gly Ala Lys Gly Ile Gly Pro Lys Thr Ala Ala Lys Leu Leu 
        195                 200                 205             


Gln Glu Tyr Gly Thr Leu Glu Lys Val Tyr Glu Ala Ala His Ala Gly 
    210                 215                 220                 


Thr Leu Lys Pro Asp Gly Thr Arg Lys Lys Leu Leu Asp Ser Glu Glu 
225                 230                 235                 240 


Asn Val Lys Phe Ser His Asp Leu Ser Cys Met Val Thr Asp Leu Pro 
                245                 250                 255     


Leu Asp Ile Glu Phe Gly Val Arg Arg Leu Pro Asp Asn Pro Leu Val 
            260                 265                 270         


Thr Glu Asp Leu Leu Thr Glu Leu Glu Leu His Ser Leu Arg Pro Met 
        275                 280                 285             


Ile Leu Gly Leu Asn Gly Pro Glu Gln Asp Gly His Ala Pro Asp Asp 
    290                 295                 300                 


Leu Leu Glu Arg Glu His Ala Gln Thr Pro Glu Glu Asp Glu Ala Ala 
305                 310                 315                 320 


Ala Leu Pro Ala Phe Ser Ala Pro Glu Leu Ala Glu Trp Gln Thr Pro 
                325                 330                 335     


Ala Glu Gly Ala Val Trp Gly Tyr Val Leu Ser Arg Glu Asp Asp Leu 
            340                 345                 350         


Thr Ala Ala Leu Leu Ala Ala Ala Thr Phe Glu Asp Gly Val Ala Arg 
        355                 360                 365             


Pro Ala Arg Val Ser Glu Pro Asp Glu Trp Ala Gln Ala Glu Ala Pro 
    370                 375                 380                 


Glu Asn Leu Phe Gly Glu Leu Leu Pro Ser Asp Lys Pro Leu Thr Lys 
385                 390                 395                 400 


Lys Glu Gln Lys Ala Leu Glu Lys Ala Gln Lys Asp Ala Glu Lys Ala 
                405                 410                 415     


Arg Ala Lys Leu Arg Glu Gln Phe Pro Ala Thr Val Asp Glu Ala Glu 
            420                 425                 430         


Phe Val Gly Gln Arg Thr Val Thr Ala Ala Ala Ala Lys Ala Leu Ala 
        435                 440                 445             


Ala His Leu Ser Val Arg Gly Thr Val Val Glu Pro Gly Asp Asp Pro 
    450                 455                 460                 


Leu Leu Tyr Ala Tyr Leu Leu Asp Pro Ala Asn Thr Asn Met Pro Val 
465                 470                 475                 480 


Val Ala Lys Arg Tyr Leu Asp Arg Glu Trp Pro Ala Asp Ala Pro Thr 
                485                 490                 495     


Arg Ala Ala Ile Thr Gly His Leu Val Arg Glu Leu Pro Pro Leu Leu 
            500                 505                 510         


Asp Asp Ala Arg Arg Lys Met Tyr Asp Glu Met Glu Lys Pro Leu Ser 
        515                 520                 525             


Gly Val Leu Gly Arg Met Glu Val Arg Gly Val Gln Val Asp Ser Asp 
    530                 535                 540                 


Phe Leu Gln Thr Leu Ser Ile Gln Ala Gly Val Arg Leu Ala Asp Leu 
545                 550                 555                 560 


Glu Ser Gln Ile His Glu Tyr Ala Gly Glu Glu Phe His Ile Arg Ser 
                565                 570                 575     


Pro Lys Gln Leu Glu Thr Val Leu Tyr Asp Lys Leu Glu Leu Ala Ser 
            580                 585                 590         


Ser Lys Lys Thr Lys Leu Thr Gly Gln Arg Ser Thr Ala Val Ser Ala 
        595                 600                 605             


Leu Glu Pro Leu Arg Asp Ala His Pro Ile Ile Pro Leu Val Leu Glu 
    610                 615                 620                 


Phe Arg Glu Leu Asp Lys Leu Arg Gly Thr Tyr Leu Asp Pro Ile Pro 
625                 630                 635                 640 


Asn Leu Val Asn Pro His Thr Gly Arg Leu His Thr Thr Phe Ala Gln 
                645                 650                 655     


Thr Ala Val Ala Thr Gly Arg Leu Ser Ser Leu Asn Pro Asn Leu Gln 
            660                 665                 670         


Asn Ile Pro Ile Arg Ser Glu Leu Gly Arg Glu Ile Arg Lys Gly Phe 
        675                 680                 685             


Ile Ala Glu Asp Gly Phe Thr Leu Ile Ala Ala Asp Tyr Ser Gln Ile 
    690                 695                 700                 


Glu Leu Arg Leu Leu Ala His Ile Ala Asp Asp Pro Leu Met Gln Gln 
705                 710                 715                 720 


Ala Phe Val Glu Gly Ala Asp Ile His Arg Arg Thr Ala Ala Gln Val 
                725                 730                 735     


Leu Gly Leu Asp Glu Ala Thr Val Asp Ala Asn Gln Arg Arg Ala Ala 
            740                 745                 750         


Lys Thr Val Asn Phe Gly Val Leu Tyr Gly Met Ser Ala His Arg Leu 
        755                 760                 765             


Ser Asn Asp Leu Gly Ile Pro Tyr Ala Glu Ala Ala Thr Phe Ile Glu 
    770                 775                 780                 


Ile Tyr Phe Ala Thr Tyr Pro Gly Ile Arg Arg Tyr Ile Asn His Thr 
785                 790                 795                 800 


Leu Asp Phe Gly Arg Thr His Gly Tyr Val Glu Thr Leu Tyr Gly Arg 
                805                 810                 815     


Arg Arg Tyr Val Pro Gly Leu Ser Ser Arg Asn Arg Val Gln Arg Glu 
            820                 825                 830         


Ala Glu Glu Arg Leu Ala Tyr Asn Met Pro Ile Gln Gly Thr Ala Ala 
        835                 840                 845             


Asp Ile Met Lys Leu Ala Met Val Gln Leu Asp Pro Gln Leu Asp Ala 
    850                 855                 860                 


Ile Gly Ala Arg Met Leu Leu Gln Val His Asp Glu Leu Leu Ile Glu 
865                 870                 875                 880 


Ala Pro Leu Asp Lys Ala Glu Gln Val Ala Ala Leu Thr Lys Lys Val 
                885                 890                 895     


Met Glu Asn Val Val Gln Leu Lys Val Pro Leu Ala Val Glu Val Gly 
            900                 905                 910         


Thr Gly Pro Asn Trp Phe Asp Thr Lys 
        915                 920     


<210>  33
<211>  892
<212>  PRT
<213>  Thermosipho africanus


<220>
<221>  MISC_FEATURE
<222>  (1)..(892)
<223>  Thermosipho africanus DNA polymerase (Taf)

<400>  33

Met Gly Lys Met Phe Leu Phe Asp Gly Thr Gly Leu Val Tyr Arg Ala 
1               5                   10                  15      


Phe Tyr Ala Ile Asp Gln Ser Leu Gln Thr Ser Ser Gly Leu His Thr 
            20                  25                  30          


Asn Ala Val Tyr Gly Leu Thr Lys Met Leu Ile Lys Phe Leu Lys Glu 
        35                  40                  45              


His Ile Ser Ile Gly Lys Asp Ala Cys Val Phe Val Leu Asp Ser Lys 
    50                  55                  60                  


Gly Gly Ser Lys Lys Arg Lys Asp Ile Leu Glu Thr Tyr Lys Ala Asn 
65                  70                  75                  80  


Arg Pro Ser Thr Pro Asp Leu Leu Leu Glu Gln Ile Pro Tyr Val Glu 
                85                  90                  95      


Glu Leu Val Asp Ala Leu Gly Ile Lys Val Leu Lys Ile Glu Gly Phe 
            100                 105                 110         


Glu Ala Asp Asp Ile Ile Ala Thr Leu Ser Lys Lys Phe Glu Ser Asp 
        115                 120                 125             


Phe Glu Lys Val Asn Ile Ile Thr Gly Asp Lys Asp Leu Leu Gln Leu 
    130                 135                 140                 


Val Ser Asp Lys Val Phe Val Trp Arg Val Glu Arg Gly Ile Thr Asp 
145                 150                 155                 160 


Leu Val Leu Tyr Asp Arg Asn Lys Val Ile Glu Lys Tyr Gly Ile Tyr 
                165                 170                 175     


Pro Glu Gln Phe Lys Asp Tyr Leu Ser Leu Val Gly Asp Gln Ile Asp 
            180                 185                 190         


Asn Ile Pro Gly Val Lys Gly Ile Gly Lys Lys Thr Ala Val Ser Leu 
        195                 200                 205             


Leu Lys Lys Tyr Asn Ser Leu Glu Asn Val Leu Lys Asn Ile Asn Leu 
    210                 215                 220                 


Leu Thr Glu Lys Leu Arg Arg Leu Leu Glu Asp Ser Lys Glu Asp Leu 
225                 230                 235                 240 


Gln Lys Ser Ile Glu Leu Val Glu Leu Ile Tyr Asp Val Pro Met Asp 
                245                 250                 255     


Val Glu Lys Asp Glu Ile Ile Tyr Arg Gly Tyr Asn Pro Asp Lys Leu 
            260                 265                 270         


Leu Lys Val Leu Lys Lys Tyr Glu Phe Ser Ser Ile Ile Lys Glu Leu 
        275                 280                 285             


Asn Leu Gln Glu Lys Leu Glu Lys Glu Tyr Ile Leu Val Asp Asn Glu 
    290                 295                 300                 


Asp Lys Leu Lys Lys Leu Ala Glu Glu Ile Glu Lys Tyr Lys Thr Phe 
305                 310                 315                 320 


Ser Ile Asp Thr Glu Thr Thr Ser Leu Asp Pro Phe Glu Ala Lys Leu 
                325                 330                 335     


Val Gly Ile Ser Ile Ser Thr Met Glu Gly Lys Ala Tyr Tyr Ile Pro 
            340                 345                 350         


Val Ser His Phe Gly Ala Lys Asn Ile Ser Lys Ser Leu Ile Asp Lys 
        355                 360                 365             


Phe Leu Lys Gln Ile Leu Gln Glu Lys Asp Tyr Asn Ile Val Gly Gln 
    370                 375                 380                 


Asn Leu Lys Phe Asp Tyr Glu Ile Phe Lys Ser Met Gly Phe Ser Pro 
385                 390                 395                 400 


Asn Val Pro His Phe Asp Thr Met Ile Ala Ala Tyr Leu Leu Asn Pro 
                405                 410                 415     


Asp Glu Lys Arg Phe Asn Leu Glu Glu Leu Ser Leu Lys Tyr Leu Gly 
            420                 425                 430         


Tyr Lys Met Ile Ser Phe Asp Glu Leu Val Asn Glu Asn Val Pro Leu 
        435                 440                 445             


Phe Gly Asn Asp Phe Ser Tyr Val Pro Leu Glu Arg Ala Val Glu Tyr 
    450                 455                 460                 


Ser Cys Glu Asp Ala Asp Val Thr Tyr Arg Ile Phe Arg Lys Leu Gly 
465                 470                 475                 480 


Arg Lys Ile Tyr Glu Asn Glu Met Glu Lys Leu Phe Tyr Glu Ile Glu 
                485                 490                 495     


Met Pro Leu Ile Asp Val Leu Ser Glu Met Glu Leu Asn Gly Val Tyr 
            500                 505                 510         


Phe Asp Glu Glu Tyr Leu Lys Glu Leu Ser Lys Lys Tyr Gln Glu Lys 
        515                 520                 525             


Met Asp Gly Ile Lys Glu Lys Val Phe Glu Ile Ala Gly Glu Thr Phe 
    530                 535                 540                 


Asn Leu Asn Ser Ser Thr Gln Val Ala Tyr Ile Leu Phe Glu Lys Leu 
545                 550                 555                 560 


Asn Ile Ala Pro Tyr Lys Lys Thr Ala Thr Gly Lys Phe Ser Thr Asn 
                565                 570                 575     


Ala Glu Val Leu Glu Glu Leu Ser Lys Glu His Glu Ile Ala Lys Leu 
            580                 585                 590         


Leu Leu Glu Tyr Arg Lys Tyr Gln Lys Leu Lys Ser Thr Tyr Ile Asp 
        595                 600                 605             


Ser Ile Pro Leu Ser Ile Asn Arg Lys Thr Asn Arg Val His Thr Thr 
    610                 615                 620                 


Phe His Gln Thr Gly Thr Ser Thr Gly Arg Leu Ser Ser Ser Asn Pro 
625                 630                 635                 640 


Asn Leu Gln Asn Leu Pro Thr Arg Ser Glu Glu Gly Lys Glu Ile Arg 
                645                 650                 655     


Lys Ala Val Arg Pro Gln Arg Gln Asp Trp Trp Ile Leu Gly Ala Asp 
            660                 665                 670         


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Val Ser Lys Asp Glu 
        675                 680                 685             


Asn Leu Leu Lys Ala Phe Lys Glu Asp Leu Asp Ile His Thr Ile Thr 
    690                 695                 700                 


Ala Ala Lys Ile Phe Gly Val Ser Glu Met Phe Val Ser Glu Gln Met 
705                 710                 715                 720 


Arg Arg Val Gly Lys Met Val Asn Phe Ala Ile Ile Tyr Gly Val Ser 
                725                 730                 735     


Pro Tyr Gly Leu Ser Lys Arg Ile Gly Leu Ser Val Ser Glu Thr Lys 
            740                 745                 750         


Lys Ile Ile Asp Asn Tyr Phe Arg Tyr Tyr Lys Gly Val Phe Glu Tyr 
        755                 760                 765             


Leu Lys Arg Met Lys Asp Glu Ala Arg Lys Lys Gly Tyr Val Thr Thr 
    770                 775                 780                 


Leu Phe Gly Arg Arg Arg Tyr Ile Pro Gln Leu Arg Ser Lys Asn Gly 
785                 790                 795                 800 


Asn Arg Val Gln Glu Gly Glu Arg Ile Ala Val Asn Thr Pro Ile Gln 
                805                 810                 815     


Gly Thr Ala Ala Asp Ile Ile Lys Ile Ala Met Ile Asn Ile His Asn 
            820                 825                 830         


Arg Leu Lys Lys Glu Asn Leu Arg Ser Lys Met Ile Leu Gln Val His 
        835                 840                 845             


Asp Glu Leu Val Phe Glu Val Pro Asp Asn Glu Leu Glu Ile Val Lys 
    850                 855                 860                 


Asp Leu Val Arg Asp Glu Met Glu Asn Ala Val Lys Leu Asp Val Pro 
865                 870                 875                 880 


Leu Lys Val Asp Val Tyr Tyr Gly Lys Glu Trp Glu 
                885                 890         


<210>  34
<211>  893
<212>  PRT
<213>  Thermotoga maritima


<220>
<221>  MISC_FEATURE
<222>  (1)..(893)
<223>  Thermotoga maritima DNA polymerase (Tma)

<400>  34

Met Ala Arg Leu Phe Leu Phe Asp Gly Thr Ala Leu Ala Tyr Arg Ala 
1               5                   10                  15      


Tyr Tyr Ala Leu Asp Arg Ser Leu Ser Thr Ser Thr Gly Ile Pro Thr 
            20                  25                  30          


Asn Ala Thr Tyr Gly Val Ala Arg Met Leu Val Arg Phe Ile Lys Asp 
        35                  40                  45              


His Ile Ile Val Gly Lys Asp Tyr Val Ala Val Ala Phe Asp Lys Lys 
    50                  55                  60                  


Ala Ala Thr Phe Arg His Lys Leu Leu Glu Thr Tyr Lys Ala Gln Arg 
65                  70                  75                  80  


Pro Lys Thr Pro Asp Leu Leu Ile Gln Gln Leu Pro Tyr Ile Lys Lys 
                85                  90                  95      


Leu Val Glu Ala Leu Gly Met Lys Val Leu Glu Val Glu Gly Tyr Glu 
            100                 105                 110         


Ala Asp Asp Ile Ile Ala Thr Leu Ala Val Lys Gly Leu Pro Leu Phe 
        115                 120                 125             


Asp Glu Ile Phe Ile Val Thr Gly Asp Lys Asp Met Leu Gln Leu Val 
    130                 135                 140                 


Asn Glu Lys Ile Lys Val Trp Arg Ile Val Lys Gly Ile Ser Asp Leu 
145                 150                 155                 160 


Glu Leu Tyr Asp Ala Gln Lys Val Lys Glu Lys Tyr Gly Val Glu Pro 
                165                 170                 175     


Gln Gln Ile Pro Asp Leu Leu Ala Leu Thr Gly Asp Glu Ile Asp Asn 
            180                 185                 190         


Ile Pro Gly Val Thr Gly Ile Gly Glu Lys Thr Ala Val Gln Leu Leu 
        195                 200                 205             


Glu Lys Tyr Lys Asp Leu Glu Asp Ile Leu Asn His Val Arg Glu Leu 
    210                 215                 220                 


Pro Gln Lys Val Arg Lys Ala Leu Leu Arg Asp Arg Glu Asn Ala Ile 
225                 230                 235                 240 


Leu Ser Lys Lys Leu Ala Ile Leu Glu Thr Asn Val Pro Ile Glu Ile 
                245                 250                 255     


Asn Trp Glu Glu Leu Arg Tyr Gln Gly Tyr Asp Arg Glu Lys Leu Leu 
            260                 265                 270         


Pro Leu Leu Lys Glu Leu Glu Phe Ala Ser Ile Met Lys Glu Leu Gln 
        275                 280                 285             


Leu Tyr Glu Glu Ser Glu Pro Val Gly Tyr Arg Ile Val Lys Asp Leu 
    290                 295                 300                 


Val Glu Phe Glu Lys Leu Ile Glu Lys Leu Arg Glu Ser Pro Ser Phe 
305                 310                 315                 320 


Ala Ile Asp Leu Glu Thr Ser Ser Leu Asp Pro Phe Asp Cys Asp Ile 
                325                 330                 335     


Val Gly Ile Ser Val Ser Phe Lys Pro Lys Glu Ala Tyr Tyr Ile Pro 
            340                 345                 350         


Leu His His Arg Asn Ala Gln Asn Leu Asp Glu Lys Glu Val Leu Lys 
        355                 360                 365             


Lys Leu Lys Glu Ile Leu Glu Asp Pro Gly Ala Lys Ile Val Gly Gln 
    370                 375                 380                 


Asn Leu Lys Phe Asp Tyr Lys Val Leu Met Val Lys Gly Val Glu Pro 
385                 390                 395                 400 


Val Pro Pro Tyr Phe Asp Thr Met Ile Ala Ala Tyr Leu Leu Glu Pro 
                405                 410                 415     


Asn Glu Lys Lys Phe Asn Leu Asp Asp Leu Ala Leu Lys Phe Leu Gly 
            420                 425                 430         


Tyr Lys Met Thr Ser Tyr Gln Glu Leu Met Ser Phe Ser Phe Pro Leu 
        435                 440                 445             


Phe Gly Phe Ser Phe Ala Asp Val Pro Val Glu Lys Ala Ala Asn Tyr 
    450                 455                 460                 


Ser Cys Glu Asp Ala Asp Ile Thr Tyr Arg Leu Tyr Lys Thr Leu Ser 
465                 470                 475                 480 


Leu Lys Leu His Glu Ala Asp Leu Glu Asn Val Phe Tyr Lys Ile Glu 
                485                 490                 495     


Met Pro Leu Val Asn Val Leu Ala Arg Met Glu Leu Asn Gly Val Tyr 
            500                 505                 510         


Val Asp Thr Glu Phe Leu Lys Lys Leu Ser Glu Glu Tyr Gly Lys Lys 
        515                 520                 525             


Leu Glu Glu Leu Ala Glu Glu Ile Tyr Arg Ile Ala Gly Glu Pro Phe 
    530                 535                 540                 


Asn Ile Asn Ser Pro Lys Gln Val Ser Arg Ile Leu Phe Glu Lys Leu 
545                 550                 555                 560 


Gly Ile Lys Pro Arg Gly Lys Thr Thr Lys Thr Gly Asp Tyr Ser Thr 
                565                 570                 575     


Arg Ile Glu Val Leu Glu Glu Leu Ala Gly Glu His Glu Ile Ile Pro 
            580                 585                 590         


Leu Ile Leu Glu Tyr Arg Lys Ile Gln Lys Leu Lys Ser Thr Tyr Ile 
        595                 600                 605             


Asp Ala Leu Pro Lys Met Val Asn Pro Lys Thr Gly Arg Ile His Ala 
    610                 615                 620                 


Ser Phe Asn Gln Thr Gly Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp 
625                 630                 635                 640 


Pro Asn Leu Gln Asn Leu Pro Thr Lys Ser Glu Glu Gly Lys Glu Ile 
                645                 650                 655     


Arg Lys Ala Ile Val Pro Gln Asp Pro Asn Trp Trp Ile Val Ser Ala 
            660                 665                 670         


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Leu Ala His Leu Ser Gly Asp 
        675                 680                 685             


Glu Asn Leu Leu Arg Ala Phe Glu Glu Gly Ile Asp Val His Thr Leu 
    690                 695                 700                 


Thr Ala Ser Arg Ile Phe Asn Val Lys Pro Glu Glu Val Thr Glu Glu 
705                 710                 715                 720 


Met Arg Arg Ala Gly Lys Met Val Asn Phe Ser Ile Ile Tyr Gly Val 
                725                 730                 735     


Thr Pro Tyr Gly Leu Ser Val Arg Leu Gly Val Pro Val Lys Glu Ala 
            740                 745                 750         


Glu Lys Met Ile Val Asn Tyr Phe Val Leu Tyr Pro Lys Val Arg Asp 
        755                 760                 765             


Tyr Ile Gln Arg Val Val Ser Glu Ala Lys Glu Lys Gly Tyr Val Arg 
    770                 775                 780                 


Thr Leu Phe Gly Arg Lys Arg Asp Ile Pro Gln Leu Met Ala Arg Asp 
785                 790                 795                 800 


Arg Asn Thr Gln Ala Glu Gly Glu Arg Ile Ala Ile Asn Thr Pro Ile 
                805                 810                 815     


Gln Gly Thr Ala Ala Asp Ile Ile Lys Leu Ala Met Ile Glu Ile Asp 
            820                 825                 830         


Arg Glu Leu Lys Glu Arg Lys Met Arg Ser Lys Met Ile Ile Gln Val 
        835                 840                 845             


His Asp Glu Leu Val Phe Glu Val Pro Asn Glu Glu Lys Asp Ala Leu 
    850                 855                 860                 


Val Glu Leu Val Lys Asp Arg Met Thr Asn Val Val Lys Leu Ser Val 
865                 870                 875                 880 


Pro Leu Glu Val Asp Val Thr Ile Gly Lys Thr Trp Ser 
                885                 890             


<210>  35
<211>  893
<212>  PRT
<213>  Thermotoga neopolitana


<220>
<221>  MISC_FEATURE
<222>  (1)..(893)
<223>  Thermotoga neopolitana DNA polymerase (Tne)

<400>  35

Met Ala Arg Leu Phe Leu Phe Asp Gly Thr Ala Leu Ala Tyr Arg Ala 
1               5                   10                  15      


Tyr Tyr Ala Leu Asp Arg Ser Leu Ser Thr Ser Thr Gly Ile Pro Thr 
            20                  25                  30          


Asn Ala Val Tyr Gly Val Ala Arg Met Leu Val Lys Phe Ile Lys Glu 
        35                  40                  45              


His Ile Ile Pro Glu Lys Asp Tyr Ala Ala Val Ala Phe Asp Lys Lys 
    50                  55                  60                  


Ala Ala Thr Phe Arg His Lys Leu Leu Val Ser Asp Lys Ala Gln Arg 
65                  70                  75                  80  


Pro Lys Thr Pro Ala Leu Leu Val Gln Gln Leu Pro Tyr Ile Lys Arg 
                85                  90                  95      


Leu Ile Glu Ala Leu Gly Phe Lys Val Leu Glu Leu Glu Gly Tyr Glu 
            100                 105                 110         


Ala Asp Asp Ile Ile Ala Thr Leu Ala Val Arg Ala Ala Arg Phe Leu 
        115                 120                 125             


Met Arg Phe Ser Leu Ile Thr Gly Asp Lys Asp Met Leu Gln Leu Val 
    130                 135                 140                 


Asn Glu Lys Ile Lys Val Trp Arg Ile Val Lys Gly Ile Ser Asp Leu 
145                 150                 155                 160 


Glu Leu Tyr Asp Ser Lys Lys Val Lys Glu Arg Tyr Gly Val Glu Pro 
                165                 170                 175     


His Gln Ile Pro Asp Leu Leu Ala Leu Thr Gly Asp Asp Ile Asp Asn 
            180                 185                 190         


Ile Pro Gly Val Thr Gly Ile Gly Glu Lys Thr Ala Val Gln Leu Leu 
        195                 200                 205             


Gly Lys Tyr Arg Asn Leu Glu Tyr Ile Leu Glu His Ala Arg Glu Leu 
    210                 215                 220                 


Pro Gln Arg Val Arg Lys Ala Leu Leu Arg Asp Arg Glu Val Ala Ile 
225                 230                 235                 240 


Leu Ser Lys Lys Leu Ala Thr Leu Val Thr Asn Ala Pro Val Glu Val 
                245                 250                 255     


Asp Trp Glu Glu Met Lys Tyr Arg Gly Tyr Asp Lys Arg Lys Leu Leu 
            260                 265                 270         


Pro Ile Leu Lys Glu Leu Glu Phe Ala Ser Ile Met Lys Glu Leu Gln 
        275                 280                 285             


Leu Tyr Glu Glu Ala Glu Pro Thr Gly Tyr Glu Ile Val Lys Asp His 
    290                 295                 300                 


Lys Thr Phe Glu Asp Leu Ile Glu Lys Leu Lys Glu Val Pro Ser Phe 
305                 310                 315                 320 


Ala Leu Asp Leu Glu Thr Ser Ser Leu Asp Pro Phe Asn Cys Glu Ile 
                325                 330                 335     


Val Gly Ile Ser Val Ser Phe Lys Pro Lys Thr Ala Tyr Tyr Ile Pro 
            340                 345                 350         


Leu His His Arg Asn Ala His Asn Leu Asp Glu Thr Leu Val Leu Ser 
        355                 360                 365             


Lys Leu Lys Glu Ile Leu Glu Asp Pro Ser Ser Lys Ile Val Gly Gln 
    370                 375                 380                 


Asn Leu Lys Tyr Asp Tyr Lys Val Leu Met Val Lys Gly Ile Ser Pro 
385                 390                 395                 400 


Val Tyr Pro His Phe Asp Thr Met Ile Ala Ala Tyr Leu Leu Glu Pro 
                405                 410                 415     


Asn Glu Lys Lys Phe Asn Leu Glu Asp Leu Ser Leu Lys Phe Leu Gly 
            420                 425                 430         


Tyr Lys Met Thr Ser Tyr Gln Glu Leu Met Ser Phe Ser Ser Pro Leu 
        435                 440                 445             


Phe Gly Phe Ser Phe Ala Asp Val Pro Val Asp Lys Ala Ala Glu Tyr 
    450                 455                 460                 


Ser Cys Glu Asp Ala Asp Ile Thr Tyr Arg Leu Tyr Lys Ile Leu Ser 
465                 470                 475                 480 


Met Lys Leu His Glu Ala Glu Leu Glu Asn Val Phe Tyr Arg Ile Glu 
                485                 490                 495     


Met Pro Leu Val Asn Val Leu Ala Arg Met Glu Phe Asn Trp Val Tyr 
            500                 505                 510         


Val Asp Thr Glu Phe Leu Lys Lys Leu Ser Glu Glu Tyr Gly Lys Lys 
        515                 520                 525             


Leu Glu Glu Leu Ala Glu Lys Ile Tyr Gln Ile Ala Gly Glu Pro Phe 
    530                 535                 540                 


Asn Ile Asn Ser Pro Lys Gln Val Ser Asn Ile Leu Phe Glu Lys Leu 
545                 550                 555                 560 


Gly Ile Lys Pro Arg Gly Lys Thr Thr Lys Thr Gly Asp Tyr Ser Thr 
                565                 570                 575     


Arg Ile Glu Val Leu Glu Glu Ile Ala Asn Glu His Glu Ile Val Pro 
            580                 585                 590         


Leu Ile Leu Glu Phe Arg Lys Ile Leu Lys Leu Lys Ser Thr Tyr Ile 
        595                 600                 605             


Asp Thr Leu Pro Lys Leu Val Asn Pro Lys Thr Gly Arg Phe His Ala 
    610                 615                 620                 


Ser Phe His Gln Thr Gly Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp 
625                 630                 635                 640 


Pro Asn Leu Gln Asn Leu Pro Thr Lys Ser Glu Glu Gly Lys Glu Ile 
                645                 650                 655     


Arg Lys Ala Ile Val Pro Gln Asp Pro Asp Trp Trp Ile Val Ser Ala 
            660                 665                 670         


Asp Tyr Ser Gln Ile Glu Leu Arg Ile Leu Ala His Leu Ser Gly Asp 
        675                 680                 685             


Glu Asn Leu Val Lys Ala Phe Glu Glu Gly Ile Asp Val His Thr Leu 
    690                 695                 700                 


Thr Ala Ser Arg Ile Tyr Asn Val Lys Pro Glu Glu Val Asn Glu Glu 
705                 710                 715                 720 


Met Arg Arg Val Gly Lys Met Val Asn Phe Ser Ile Ile Tyr Gly Val 
                725                 730                 735     


Thr Pro Tyr Gly Leu Ser Val Arg Leu Gly Ile Pro Val Lys Glu Ala 
            740                 745                 750         


Glu Lys Met Ile Ile Ser Tyr Phe Thr Leu Tyr Pro Lys Val Arg Ser 
        755                 760                 765             


Tyr Ile Gln Gln Val Val Ala Glu Ala Lys Glu Lys Gly Tyr Val Arg 
    770                 775                 780                 


Thr Leu Phe Gly Arg Lys Arg Asp Ile Pro Gln Leu Met Ala Arg Asp 
785                 790                 795                 800 


Lys Asn Thr Gln Ser Glu Gly Glu Arg Ile Ala Ile Asn Thr Pro Ile 
                805                 810                 815     


Gln Gly Thr Ala Ala Asp Ile Ile Lys Leu Ala Met Ile Asp Ile Asp 
            820                 825                 830         


Glu Glu Leu Arg Lys Arg Asn Met Lys Ser Arg Met Ile Ile Gln Val 
        835                 840                 845             


His Asp Glu Leu Val Phe Glu Val Pro Asp Glu Glu Lys Glu Glu Leu 
    850                 855                 860                 


Val Asp Leu Val Lys Asn Lys Met Thr Asn Val Val Lys Leu Ser Val 
865                 870                 875                 880 


Pro Leu Glu Val Asp Ile Ser Ile Gly Lys Ser Trp Ser 
                885                 890             


<210>  36
<211>  876
<212>  PRT
<213>  Bacillus stearothermophilus


<220>
<221>  MISC_FEATURE
<222>  (1)..(876)
<223>  Bacillus stearothermophilus DNA polymerase (Bst)

<400>  36

Met Lys Asn Lys Leu Val Leu Ile Asp Gly Asn Ser Val Ala Tyr Arg 
1               5                   10                  15      


Ala Phe Phe Ala Leu Pro Leu Leu His Asn Asp Lys Gly Ile His Thr 
            20                  25                  30          


Asn Ala Val Tyr Gly Phe Thr Met Met Leu Asn Lys Ile Leu Ala Glu 
        35                  40                  45              


Glu Gln Pro Thr His Ile Leu Val Ala Phe Asp Ala Gly Lys Thr Thr 
    50                  55                  60                  


Phe Arg His Glu Thr Phe Gln Asp Tyr Lys Gly Gly Arg Gln Gln Thr 
65                  70                  75                  80  


Pro Pro Glu Leu Ser Glu Gln Phe Pro Leu Leu Arg Glu Leu Leu Lys 
                85                  90                  95      


Ala Tyr Arg Ile Pro Ala Tyr Glu Leu Asp His Tyr Glu Ala Asp Asp 
            100                 105                 110         


Ile Ile Gly Thr Met Ala Ala Arg Ala Glu Arg Glu Gly Phe Ala Val 
        115                 120                 125             


Lys Val Ile Ser Gly Asp Arg Asp Leu Thr Gln Leu Ala Ser Pro Gln 
    130                 135                 140                 


Val Thr Val Glu Ile Thr Lys Lys Gly Ile Thr Asp Ile Glu Ser Tyr 
145                 150                 155                 160 


Thr Pro Glu Thr Val Val Glu Lys Tyr Gly Leu Thr Pro Glu Gln Ile 
                165                 170                 175     


Val Asp Leu Lys Gly Leu Met Gly Asp Lys Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Ile Gly Glu Lys Thr Ala Val Lys Leu Leu Lys Gln Phe 
        195                 200                 205             


Gly Thr Val Glu Asn Val Leu Ala Ser Ile Asp Glu Ile Lys Gly Glu 
    210                 215                 220                 


Lys Leu Lys Glu Asn Leu Arg Gln Tyr Arg Asp Leu Ala Leu Leu Ser 
225                 230                 235                 240 


Lys Gln Leu Ala Ala Ile Cys Arg Asp Ala Pro Val Glu Leu Thr Leu 
                245                 250                 255     


Asp Asp Ile Val Tyr Lys Gly Glu Asp Arg Glu Lys Val Val Ala Leu 
            260                 265                 270         


Phe Gln Glu Leu Gly Phe Gln Ser Phe Leu Asp Lys Met Ala Val Gln 
        275                 280                 285             


Thr Asp Glu Gly Glu Lys Pro Leu Ala Gly Met Asp Phe Ala Ile Ala 
    290                 295                 300                 


Asp Ser Val Thr Asp Glu Met Leu Ala Asp Lys Ala Ala Leu Val Val 
305                 310                 315                 320 


Glu Val Val Gly Asp Asn Tyr His His Ala Pro Ile Val Gly Ile Ala 
                325                 330                 335     


Leu Ala Asn Glu Arg Gly Arg Phe Phe Leu Arg Pro Glu Thr Ala Leu 
            340                 345                 350         


Ala Asp Pro Lys Phe Leu Ala Trp Leu Gly Asp Glu Thr Lys Lys Lys 
        355                 360                 365             


Thr Met Phe Asp Ser Lys Arg Ala Ala Val Ala Leu Lys Trp Lys Gly 
    370                 375                 380                 


Ile Glu Leu Arg Gly Val Val Phe Asp Leu Leu Leu Ala Ala Tyr Leu 
385                 390                 395                 400 


Leu Asp Pro Ala Gln Ala Ala Gly Asp Val Ala Ala Val Ala Lys Met 
                405                 410                 415     


His Gln Tyr Glu Ala Val Arg Ser Asp Glu Ala Val Tyr Gly Lys Gly 
            420                 425                 430         


Ala Lys Arg Thr Val Pro Asp Glu Pro Thr Leu Ala Glu His Leu Ala 
        435                 440                 445             


Arg Lys Ala Ala Ala Ile Trp Ala Leu Glu Glu Pro Leu Met Asp Glu 
    450                 455                 460                 


Leu Arg Arg Asn Glu Gln Asp Arg Leu Leu Thr Glu Leu Glu Gln Pro 
465                 470                 475                 480 


Leu Ala Gly Ile Leu Ala Asn Met Glu Phe Thr Gly Val Lys Val Asp 
                485                 490                 495     


Thr Lys Arg Leu Glu Gln Met Gly Ala Glu Leu Thr Glu Gln Leu Gln 
            500                 505                 510         


Ala Val Glu Arg Arg Ile Tyr Glu Leu Ala Gly Gln Glu Phe Asn Ile 
        515                 520                 525             


Asn Ser Pro Lys Gln Leu Gly Thr Val Leu Phe Asp Lys Leu Gln Leu 
    530                 535                 540                 


Pro Val Leu Lys Lys Thr Lys Thr Gly Tyr Ser Thr Ser Ala Asp Val 
545                 550                 555                 560 


Leu Glu Lys Leu Ala Pro His His Glu Ile Val Glu His Ile Leu His 
                565                 570                 575     


Tyr Arg Gln Leu Gly Lys Leu Gln Ser Thr Tyr Ile Glu Gly Leu Leu 
            580                 585                 590         


Lys Val Val His Pro Val Thr Gly Lys Val His Thr Met Phe Asn Gln 
        595                 600                 605             


Ala Leu Thr Gln Thr Gly Arg Leu Ser Ser Val Glu Pro Asn Leu Gln 
    610                 615                 620                 


Asn Ile Pro Ile Arg Leu Glu Glu Gly Arg Lys Ile Arg Gln Ala Phe 
625                 630                 635                 640 


Val Pro Ser Glu Pro Asp Trp Leu Ile Phe Ala Ala Asp Tyr Ser Gln 
                645                 650                 655     


Ile Glu Leu Arg Val Leu Ala His Ile Ala Glu Asp Asp Asn Leu Ile 
            660                 665                 670         


Glu Ala Phe Arg Arg Gly Leu Asp Ile His Thr Lys Thr Ala Met Asp 
        675                 680                 685             


Ile Phe His Val Ser Glu Glu Asp Val Thr Ala Asn Met Arg Arg Gln 
    690                 695                 700                 


Ala Lys Ala Val Asn Phe Gly Ile Val Tyr Gly Ile Ser Asp Tyr Gly 
705                 710                 715                 720 


Leu Ala Gln Asn Leu Asn Ile Thr Arg Lys Glu Ala Ala Glu Phe Ile 
                725                 730                 735     


Glu Arg Tyr Phe Ala Ser Phe Pro Gly Val Lys Gln Tyr Met Asp Asn 
            740                 745                 750         


Ile Val Gln Glu Ala Lys Gln Lys Gly Tyr Val Thr Thr Leu Leu His 
        755                 760                 765             


Arg Arg Arg Tyr Leu Pro Asp Ile Thr Ser Arg Asn Phe Asn Val Arg 
    770                 775                 780                 


Ser Phe Ala Glu Arg Thr Ala Met Asn Thr Pro Ile Gln Gly Ser Ala 
785                 790                 795                 800 


Ala Asp Ile Ile Lys Lys Ala Met Ile Asp Leu Ser Val Arg Leu Arg 
                805                 810                 815     


Glu Glu Arg Leu Gln Ala Arg Leu Leu Leu Gln Val His Asp Glu Leu 
            820                 825                 830         


Ile Leu Glu Ala Pro Lys Glu Glu Ile Glu Arg Leu Cys Arg Leu Val 
        835                 840                 845             


Pro Glu Val Met Glu Gln Ala Val Ala Leu Arg Val Pro Leu Lys Val 
    850                 855                 860                 


Asp Tyr His Tyr Gly Pro Thr Trp Tyr Asp Ala Lys 
865                 870                 875     


<210>  37
<211>  877
<212>  PRT
<213>  Bacillus caldotenax


<220>
<221>  MISC_FEATURE
<222>  (1)..(877)
<223>  Bacillus caldotenax DNA polymerase (Bca)

<400>  37

Met Lys Lys Lys Leu Val Leu Ile Asp Gly Ser Ser Val Ala Tyr Arg 
1               5                   10                  15      


Ala Phe Phe Ala Leu Pro Leu Leu His Asn Asp Lys Gly Ile His Thr 
            20                  25                  30          


Asn Ala Val Tyr Gly Phe Thr Met Met Leu Asn Lys Ile Leu Ala Glu 
        35                  40                  45              


Glu Glu Pro Thr His Met Leu Val Ala Phe Asp Ala Gly Lys Thr Thr 
    50                  55                  60                  


Phe Arg His Glu Ala Phe Gln Glu Tyr Lys Gly Gly Arg Gln Gln Thr 
65                  70                  75                  80  


Pro Pro Glu Leu Ser Glu Gln Phe Pro Leu Leu Arg Glu Leu Leu Arg 
                85                  90                  95      


Ala Tyr Arg Ile Pro Ala Tyr Glu Leu Glu Asn Tyr Glu Ala Asp Asp 
            100                 105                 110         


Ile Ile Gly Thr Leu Ala Ala Arg Ala Glu Gln Glu Gly Phe Glu Val 
        115                 120                 125             


Lys Val Ile Ser Gly Asp Arg Asp Leu Thr Gln Leu Ala Ser Pro His 
    130                 135                 140                 


Val Thr Val Asp Ile Thr Lys Lys Gly Ile Thr Asp Ile Glu Pro Tyr 
145                 150                 155                 160 


Thr Pro Glu Ala Val Arg Glu Lys Tyr Gly Leu Thr Pro Glu Gln Ile 
                165                 170                 175     


Val Asp Leu Lys Gly Leu Met Gly Asp Lys Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Ile Gly Glu Lys Thr Ala Val Lys Leu Leu Arg Gln Phe 
        195                 200                 205             


Gly Thr Val Glu Asn Val Leu Ala Ser Ile Asp Glu Ile Lys Gly Glu 
    210                 215                 220                 


Lys Leu Lys Glu Thr Leu Arg Gln His Arg Glu Met Ala Leu Leu Ser 
225                 230                 235                 240 


Lys Lys Leu Ala Ala Ile Arg Arg Asp Ala Pro Val Glu Leu Ser Leu 
                245                 250                 255     


Asp Asp Ile Ala Tyr Gln Gly Glu Asp Arg Glu Lys Val Val Ala Leu 
            260                 265                 270         


Phe Lys Glu Leu Gly Phe Gln Ser Phe Leu Glu Lys Met Glu Ser Pro 
        275                 280                 285             


Ser Ser Glu Glu Glu Lys Pro Leu Ala Lys Met Ala Phe Thr Leu Ala 
    290                 295                 300                 


Asp Arg Val Thr Glu Glu Met Leu Ala Asp Lys Ala Ala Leu Val Val 
305                 310                 315                 320 


Glu Val Val Glu Glu Asn Tyr His Asp Ala Pro Ile Val Gly Ile Ala 
                325                 330                 335     


Val Val Asn Glu His Gly Arg Phe Phe Leu Arg Pro Glu Thr Ala Leu 
            340                 345                 350         


Ala Asp Pro Gln Phe Val Ala Trp Leu Gly Asp Glu Thr Lys Lys Lys 
        355                 360                 365             


Ser Met Phe Asp Ser Lys Arg Ala Ala Val Ala Leu Lys Trp Lys Gly 
    370                 375                 380                 


Ile Glu Leu Cys Gly Val Ser Phe Asp Leu Leu Leu Ala Ala Tyr Leu 
385                 390                 395                 400 


Leu Asp Pro Ala Gln Gly Val Asp Asp Val Ala Ala Ala Ala Lys Met 
                405                 410                 415     


Lys Gln Tyr Glu Ala Val Arg Pro Asp Glu Ala Val Tyr Gly Lys Gly 
            420                 425                 430         


Ala Lys Arg Ala Val Pro Asp Glu Pro Val Leu Ala Glu His Leu Val 
        435                 440                 445             


Arg Lys Ala Ala Ala Ile Trp Ala Leu Glu Arg Pro Phe Leu Asp Glu 
    450                 455                 460                 


Leu Arg Arg Asn Glu Gln Asp Arg Leu Leu Val Glu Leu Glu Gln Pro 
465                 470                 475                 480 


Leu Ser Ser Ile Leu Ala Glu Met Glu Phe Ala Gly Val Lys Val Asp 
                485                 490                 495     


Thr Lys Arg Leu Glu Gln Met Gly Glu Glu Leu Ala Glu Gln Leu Arg 
            500                 505                 510         


Thr Val Glu Gln Arg Ile Tyr Glu Leu Ala Gly Gln Glu Phe Asn Ile 
        515                 520                 525             


Asn Ser Pro Lys Gln Leu Gly Val Ile Leu Phe Glu Lys Leu Gln Leu 
    530                 535                 540                 


Pro Val Leu Lys Lys Ser Lys Thr Gly Tyr Ser Thr Ser Ala Asp Val 
545                 550                 555                 560 


Leu Glu Lys Leu Ala Pro Tyr His Glu Ile Val Glu Asn Ile Leu Gln 
                565                 570                 575     


His Tyr Arg Gln Leu Gly Lys Leu Gln Ser Thr Tyr Ile Glu Gly Leu 
            580                 585                 590         


Leu Lys Val Val Arg Pro Asp Thr Lys Lys Val His Thr Ile Phe Asn 
        595                 600                 605             


Gln Ala Leu Thr Gln Thr Gly Arg Leu Ser Ser Thr Glu Pro Asn Leu 
    610                 615                 620                 


Gln Asn Ile Pro Ile Arg Leu Glu Glu Gly Arg Lys Ile Arg Gln Ala 
625                 630                 635                 640 


Phe Val Pro Ser Glu Ser Asp Trp Leu Ile Phe Ala Ala Asp Tyr Ser 
                645                 650                 655     


Gln Ile Glu Leu Arg Val Leu Ala His Ile Ala Glu Asp Asp Asn Leu 
            660                 665                 670         


Met Glu Ala Phe Arg Arg Asp Leu Asp Ile His Thr Lys Thr Ala Met 
        675                 680                 685             


Asp Ile Phe Gln Val Ser Glu Asp Glu Val Thr Pro Asn Met Arg Arg 
    690                 695                 700                 


Gln Ala Lys Ala Val Asn Phe Gly Ile Val Tyr Gly Ile Ser Asp Tyr 
705                 710                 715                 720 


Gly Leu Ala Gln Asn Leu Asn Ile Ser Arg Lys Glu Ala Ala Glu Phe 
                725                 730                 735     


Ile Glu Arg Tyr Phe Glu Ser Phe Pro Gly Val Lys Arg Tyr Met Glu 
            740                 745                 750         


Asn Ile Val Gln Glu Ala Lys Gln Lys Gly Tyr Val Thr Thr Leu Leu 
        755                 760                 765             


His Arg Arg Arg Tyr Leu Pro Asp Ile Thr Ser Arg Asn Phe Asn Val 
    770                 775                 780                 


Arg Ser Phe Ala Glu Arg Met Ala Met Asn Thr Pro Ile Gln Gly Ser 
785                 790                 795                 800 


Ala Ala Asp Ile Ile Lys Lys Ala Met Ile Asp Leu Asn Ala Arg Leu 
                805                 810                 815     


Lys Glu Glu Arg Leu Gln Ala Arg Leu Leu Leu Gln Val His Asp Glu 
            820                 825                 830         


Leu Ile Leu Glu Ala Pro Lys Glu Glu Met Glu Arg Leu Cys Arg Leu 
        835                 840                 845             


Val Pro Glu Val Met Glu Gln Ala Val Thr Leu Arg Val Pro Leu Lys 
    850                 855                 860                 


Val Asp Tyr His Tyr Gly Ser Thr Trp Tyr Asp Ala Lys 
865                 870                 875         


<210>  38
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polymerase motif corresponding to the D580X mutation of
       Z05


<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  Xaa = Ser or Thr

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Xaa = any amino acid other than Asp or Glu

<400>  38

Thr Gly Arg Leu Ser Ser Xaa Xaa Pro Asn Leu Gln Asn 
1               5                   10              


<210>  39
<211>  831
<212>  PRT
<213>  Carboxydothermus hydrogenoformans


<220>
<221>  MISC_FEATURE
<222>  (1)..(831)
<223>  Carboxydothermus hydrogenoformans DNA polymerase (Chy)

<400>  39

Met Gly Lys Val Val Leu Val Asp Gly Asn Ser Leu Leu His Arg Ala 
1               5                   10                  15      


Phe Phe Ala Leu Pro Pro Leu Lys Thr Thr Lys Gly Glu Pro Thr Gly 
            20                  25                  30          


Ala Val Tyr Glu Phe Leu Thr Met Leu Phe Arg Val Ile Lys Asp Glu 
        35                  40                  45              


Lys Pro Glu Tyr Leu Ala Val Ala Phe Asp Ile Ser Arg Lys Thr Phe 
    50                  55                  60                  


Arg Thr Glu Gln Phe Thr Ala Tyr Lys Gly His Arg Lys Glu Ala Pro 
65                  70                  75                  80  


Asp Glu Leu Val Pro Gln Phe Ala Leu Val Arg Glu Val Leu Lys Val 
                85                  90                  95      


Leu Asn Val Pro Tyr Ile Glu Leu Asp Gly Tyr Glu Ala Asp Asp Ile 
            100                 105                 110         


Ile Gly His Leu Ser Arg Ala Phe Ala Gly Gln Gly His Glu Val Val 
        115                 120                 125             


Ile Tyr Thr Ala Asp Arg Asp Met Leu Gln Leu Val Asp Glu Lys Thr 
    130                 135                 140                 


Val Val Tyr Leu Thr Lys Lys Gly Ile Thr Glu Leu Val Lys Met Asp 
145                 150                 155                 160 


Leu Ala Ala Ile Leu Glu Asn Tyr Gly Leu Lys Pro Lys Gln Leu Val 
                165                 170                 175     


Asp Val Lys Gly Leu Met Gly Asp Pro Ser Asp Asn Ile Pro Gly Val 
            180                 185                 190         


Pro Gly Ile Gly Glu Lys Thr Ala Leu Asp Leu Ile Lys Thr Tyr Gly 
        195                 200                 205             


Ser Val Glu Glu Val Leu Ala Arg Lys Asp Glu Leu Lys Pro Lys Leu 
    210                 215                 220                 


Arg Glu Lys Leu Ala Glu His Glu Asn Leu Ala Lys Ile Ser Lys Gln 
225                 230                 235                 240 


Leu Ala Thr Ile Leu Arg Glu Ile Pro Leu Glu Ile Ser Leu Glu Asp 
                245                 250                 255     


Leu Lys Val Lys Glu Pro Asn Tyr Glu Glu Val Ala Lys Leu Phe Leu 
            260                 265                 270         


His Leu Glu Phe Lys Ser Phe Leu Lys Glu Ile Glu Pro Lys Ile Lys 
        275                 280                 285             


Lys Glu Tyr Gln Glu Gly Lys Asp Leu Val Gln Val Glu Thr Val Glu 
    290                 295                 300                 


Thr Glu Gly Gln Ile Ala Val Val Phe Ser Asp Gly Phe Tyr Val Asp 
305                 310                 315                 320 


Asp Gly Glu Lys Thr Lys Phe Tyr Ser Leu Asp Arg Leu Asn Glu Ile 
                325                 330                 335     


Glu Glu Ile Phe Arg Asn Lys Lys Ile Ile Thr Asp Asp Ala Lys Gly 
            340                 345                 350         


Ile Tyr His Val Cys Leu Glu Lys Gly Leu Thr Phe Pro Glu Val Cys 
        355                 360                 365             


Phe Asp Ala Arg Ile Ala Ala Tyr Val Leu Asn Pro Ala Asp Gln Asn 
    370                 375                 380                 


Pro Gly Leu Lys Gly Leu Tyr Leu Lys Tyr Asp Leu Pro Val Tyr Glu 
385                 390                 395                 400 


Asp Val Ser Leu Asn Ile Arg Gly Leu Phe Tyr Leu Lys Lys Glu Met 
                405                 410                 415     


Met Arg Lys Ile Phe Glu Gln Glu Gln Glu Arg Leu Phe Tyr Glu Ile 
            420                 425                 430         


Glu Leu Pro Leu Thr Pro Val Leu Ala Gln Met Glu His Thr Gly Ile 
        435                 440                 445             


Gln Val Asp Arg Glu Ala Leu Lys Glu Met Ser Leu Glu Leu Gly Glu 
    450                 455                 460                 


Gln Ile Glu Glu Leu Ile Arg Glu Ile Tyr Val Leu Ala Gly Glu Glu 
465                 470                 475                 480 


Phe Asn Leu Asn Ser Pro Arg Gln Leu Gly Val Ile Leu Phe Glu Lys 
                485                 490                 495     


Leu Gly Leu Pro Val Ile Lys Lys Thr Lys Thr Gly Tyr Ser Thr Asp 
            500                 505                 510         


Ala Glu Val Leu Glu Glu Leu Leu Pro Phe His Glu Ile Ile Gly Lys 
        515                 520                 525             


Ile Leu Asn Tyr Arg Gln Leu Met Lys Leu Lys Ser Thr Tyr Thr Asp 
    530                 535                 540                 


Gly Leu Met Pro Leu Ile Asn Glu Arg Thr Gly Lys Leu His Thr Thr 
545                 550                 555                 560 


Phe Asn Gln Thr Gly Thr Leu Thr Gly Arg Leu Ala Ser Ser Glu Pro 
                565                 570                 575     


Asn Leu Gln Asn Ile Pro Ile Arg Leu Glu Leu Gly Arg Lys Leu Arg 
            580                 585                 590         


Lys Met Phe Ile Pro Ser Pro Gly Tyr Asp Tyr Ile Val Ser Ala Asp 
        595                 600                 605             


Tyr Ser Gln Ile Glu Leu Arg Leu Leu Ala His Phe Ser Glu Glu Pro 
    610                 615                 620                 


Lys Leu Ile Glu Ala Tyr Gln Lys Gly Glu Asp Ile His Arg Lys Thr 
625                 630                 635                 640 


Ala Ser Glu Val Phe Gly Val Ser Leu Glu Glu Val Thr Pro Glu Met 
                645                 650                 655     


Arg Ala His Ala Lys Ser Val Asn Phe Gly Ile Val Tyr Gly Ile Ser 
            660                 665                 670         


Asp Phe Gly Leu Gly Arg Asp Leu Lys Ile Pro Arg Glu Val Ala Gly 
        675                 680                 685             


Lys Tyr Ile Lys Asn Tyr Phe Ala Asn Tyr Pro Lys Val Arg Glu Tyr 
    690                 695                 700                 


Leu Asp Glu Leu Val Arg Thr Ala Arg Glu Lys Gly Tyr Val Thr Thr 
705                 710                 715                 720 


Leu Phe Gly Arg Arg Arg Tyr Ile Pro Glu Leu Ser Ser Lys Asn Arg 
                725                 730                 735     


Thr Val Gln Gly Phe Gly Glu Arg Thr Ala Met Asn Thr Pro Leu Gln 
            740                 745                 750         


Gly Ser Ala Ala Asp Ile Ile Lys Leu Ala Met Ile Asn Val Glu Lys 
        755                 760                 765             


Glu Leu Lys Ala Arg Lys Leu Lys Ser Arg Leu Leu Leu Ser Val His 
    770                 775                 780                 


Asp Glu Leu Val Leu Glu Val Pro Ala Glu Glu Leu Glu Glu Val Lys 
785                 790                 795                 800 


Ala Leu Val Lys Gly Val Met Glu Ser Val Val Glu Leu Lys Val Pro 
                805                 810                 815     


Leu Ile Ala Glu Val Gly Ala Gly Lys Asn Trp Tyr Glu Ala Lys 
            820                 825                 830     


<210>  40
<211>  834
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic G42E C21 DNA Polymerase

<400>  40

Met Lys Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Glu Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Tyr Lys Ala Val Phe 
    50                  55                  60                  


Val Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu 
65                  70                  75                  80  


Ala Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln 
                85                  90                  95      


Leu Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Phe Thr Arg Leu 
            100                 105                 110         


Glu Val Pro Gly Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys 
        115                 120                 125             


Lys Ala Glu Arg Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg 
    130                 135                 140                 


Asp Leu Tyr Gln Leu Val Ser Asp Arg Val Ala Val Leu His Pro Glu 
145                 150                 155                 160 


Gly His Leu Ile Thr Pro Glu Trp Leu Trp Glu Lys Tyr Gly Leu Lys 
                165                 170                 175     


Pro Glu Gln Trp Val Asp Phe Arg Ala Leu Val Gly Asp Pro Ser Asp 
            180                 185                 190         


Asn Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu 
        195                 200                 205             


Leu Lys Glu Trp Gly Ser Leu Glu Asn Ile Leu Lys Asn Leu Asp Arg 
    210                 215                 220                 


Val Lys Pro Glu Ser Val Arg Glu Arg Ile Lys Ala His Leu Glu Asp 
225                 230                 235                 240 


Leu Lys Leu Ser Leu Glu Leu Ser Arg Val Arg Ser Asp Leu Pro Leu 
                245                 250                 255     


Glu Val Asp Phe Ala Arg Arg Arg Glu Pro Asp Arg Glu Gly Leu Arg 
            260                 265                 270         


Ala Phe Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             


Leu Leu Glu Ala Pro Ala Pro Leu Glu Glu Ala Pro Trp Pro Pro Pro 
    290                 295                 300                 


Glu Gly Ala Phe Val Gly Phe Val Leu Ser Arg Pro Glu Pro Met Trp 
305                 310                 315                 320 


Ala Glu Leu Lys Ala Leu Ala Ala Cys Lys Glu Gly Arg Val His Arg 
                325                 330                 335     


Ala Lys Asp Pro Leu Ala Gly Leu Lys Asp Leu Lys Glu Val Arg Gly 
            340                 345                 350         


Leu Leu Ala Lys Asp Leu Ala Val Leu Ala Leu Arg Glu Gly Leu Asp 
        355                 360                 365             


Leu Ala Pro Ser Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro 
    370                 375                 380                 


Ser Asn Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp 
385                 390                 395                 400 


Thr Glu Asp Ala Ala His Arg Ala Leu Leu Ala Glu Arg Leu Gln Gln 
                405                 410                 415     


Asn Leu Leu Glu Arg Leu Lys Gly Glu Glu Lys Leu Leu Trp Leu Tyr 
            420                 425                 430         


Gln Glu Val Glu Lys Pro Leu Ser Arg Val Leu Ala His Met Glu Ala 
        435                 440                 445             


Thr Gly Val Arg Leu Asp Val Ala Tyr Leu Lys Ala Leu Ser Leu Glu 
    450                 455                 460                 


Leu Ala Glu Glu Ile Arg Arg Leu Glu Glu Glu Val Phe Arg Leu Ala 
465                 470                 475                 480 


Gly His Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu 
                485                 490                 495     


Phe Asp Glu Leu Arg Leu Pro Ala Leu Gly Lys Thr Gln Lys Thr Gly 
            500                 505                 510         


Lys Arg Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His 
        515                 520                 525             


Pro Ile Val Glu Lys Ile Leu Gln His Arg Glu Leu Thr Lys Leu Lys 
    530                 535                 540                 


Asn Thr Tyr Val Asp Pro Leu Pro Gly Leu Val His Pro Arg Thr Gly 
545                 550                 555                 560 


Arg Leu His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu 
                565                 570                 575     


Ser Ser Ser Asp Pro Asn Leu Gln Asn Ile Pro Ile Arg Thr Pro Leu 
            580                 585                 590         


Gly Gln Arg Ile Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu 
        595                 600                 605             


Val Ala Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu 
    610                 615                 620                 


Ser Gly Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Lys Asp Ile 
625                 630                 635                 640 


His Thr Gln Thr Ala Ser Trp Met Phe Gly Val Ser Pro Glu Ala Val 
                645                 650                 655     


Asp Pro Leu Met Arg Arg Ala Ala Lys Thr Val Asn Phe Gly Val Leu 
            660                 665                 670         


Tyr Gly Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr 
        675                 680                 685             


Glu Glu Ala Val Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys 
    690                 695                 700                 


Val Arg Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Lys Arg Gly 
705                 710                 715                 720 


Tyr Val Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn 
                725                 730                 735     


Ala Arg Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn 
            740                 745                 750         


Met Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val 
        755                 760                 765             


Lys Leu Phe Pro His Leu Arg Glu Met Gly Ala Arg Met Leu Leu Gln 
    770                 775                 780                 


Val His Asp Glu Leu Leu Leu Glu Ala Pro Gln Ala Arg Ala Glu Glu 
785                 790                 795                 800 


Val Ala Ala Leu Ala Lys Glu Ala Met Glu Lys Ala Tyr Pro Leu Ala 
                805                 810                 815     


Val Pro Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala 
            820                 825                 830         


Lys Gly 
        


<210>  41
<211>  2505
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Nucleic acid sequence encoding C21 polymerase

<400>  41
atgaaagcta tgttaccatt attcgaaccc aaaggccggg tcctcctggt ggacggccac       60

cacctggcct accgcacctt cttcgccctg aagggcctca ccacgagccg gggcgaaccg      120

gtgcaggcgg tttacggctt cgccaagagc ctcctcaagg ccctgaagga ggacgggtac      180

aaggccgtct tcgtggtctt tgacgccaag gccccttcct tccgccacga ggcctacgag      240

gcctacaagg caggccgcgc cccgaccccc gaggacttcc cccggcagct cgccctcatc      300

aaggagctgg tggacctcct ggggtttact cgcctcgagg ttccgggctt tgaggcggac      360

gacgtcctcg ccaccctggc caagaaggcg gaaagggagg ggtacgaggt gcgcatcctc      420

accgccgacc gggaccttta ccagctcgtc tccgaccgcg tcgccgtcct ccaccccgag      480

ggccacctca tcaccccgga gtggctttgg gagaagtacg gccttaagcc ggagcagtgg      540

gtggacttcc gcgccctcgt gggggacccc tccgacaacc tccccggggt caagggcatc      600

ggggagaaga ccgccctcaa gctcctcaag gagtggggaa gcctggaaaa tatcctcaag      660

aacctggacc gggtgaagcc ggaaagcgtc cgggaaagga tcaaggccca cctggaagac      720

cttaagctct ccttggagct ttcccgggtg cgctcggacc tccccctgga ggtggacttc      780

gcccggaggc gggagcctga ccgggaaggg cttcgggcct ttttggagcg cttggagttc      840

ggcagcctcc tccacgagtt cggcctcctc gaggcccccg cccccctgga ggaggccccc      900

tggcccccgc cggaaggggc cttcgtgggc ttcgtcctct cccgccccga gcccatgtgg      960

gcggagctta aagccctggc cgcctgcaag gagggccggg tgcaccgggc aaaggacccc     1020

ttggcggggc taaaggacct caaggaggtc cgaggcctcc tcgccaagga cctcgccgtt     1080

ttggcccttc gcgaggggct ggacctcgcg ccttcggacg accccatgct cctcgcctac     1140

ctcctggacc cctccaacac cacccccgag ggggtggccc ggcgctacgg gggggagtgg     1200

acggaggacg ccgcccaccg ggccctcctc gctgagcggc tccagcaaaa cctcttggaa     1260

cgcctcaagg gagaggaaaa gctcctttgg ctctaccaag aggtggaaaa gcccctctcc     1320

cgggtcctgg cccacatgga ggccaccggg gtaaggctgg acgtggccta tctaaaggcc     1380

ctttccctgg agcttgcgga ggagattcgc cgcctcgagg aggaggtctt ccgcctggcg     1440

ggccacccct tcaacctgaa ctcccgtgac cagctagagc gggtgctctt tgacgagctt     1500

aggcttcccg ccctgggcaa gacgcaaaag acggggaagc gctccaccag cgccgcggtg     1560

ctggaggccc tcagggaggc ccaccccatc gtggagaaga tcctccagca ccgggagctc     1620

accaagctca agaacaccta cgtagacccc ctcccgggcc tcgtccaccc gaggacgggc     1680

cgcctccaca cccgcttcaa ccagacagcc acggccacgg gaaggctctc tagctccggg     1740

cccaacctgc agaacatccc catccgcacc cccttgggcc agaggatccg ccgggccttc     1800

gtggccgagg cgggatgggc gttggtggcc ctggactata gccagatgga gctccgggtc     1860

ctcgcccacc tctccgggga cgagaacctg atcagggtct tccaggaggg gaaggacatc     1920

cacacccaga ccgcaagctg gatgttcggc gtctccccgg aggccgtgga ccccctgatg     1980

cgccgggcgg ccaagacggt gaacttcggc gtcctctacg gcatgtccgc ccataggctc     2040

tcccaggagc ttgccatccc ctacgaggag gcggtggcct ttatagagcg ctacttccaa     2100

agcttcccca aggtgcgcgc ctggaaagaa aagaccctgg aggaggggag gaagcggggc     2160

tacgtggaaa ccctcttcgg aagaaggcgc tacgtgcccg acctcaacgc ccgggtgaag     2220

agcgtcaggg aggccgcgga gcgcatggcc ttcaacatgc ccgtccaggg caccgccgcc     2280

gacctcatga agctcgccat ggtgaagctc ttcccccacc tccgggagat gggggcccgc     2340

atgctcctcc aggtccacga cgagctcctc ctggaggccc cccaagcgcg ggccgaggag     2400

gtggcggctt tggccaagga ggccatggag aaggcctacc ccctcgccgt gcccctggag     2460

gtggaggtgg ggatcgggga ggactggctt tccgccaagg gctga                     2505


<210>  42
<211>  834
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic C21 polymerase with I709K mutation

<400>  42

Met Lys Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Tyr Lys Ala Val Phe 
    50                  55                  60                  


Val Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu 
65                  70                  75                  80  


Ala Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln 
                85                  90                  95      


Leu Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Phe Thr Arg Leu 
            100                 105                 110         


Glu Val Pro Gly Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys 
        115                 120                 125             


Lys Ala Glu Arg Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg 
    130                 135                 140                 


Asp Leu Tyr Gln Leu Val Ser Asp Arg Val Ala Val Leu His Pro Glu 
145                 150                 155                 160 


Gly His Leu Ile Thr Pro Glu Trp Leu Trp Glu Lys Tyr Gly Leu Lys 
                165                 170                 175     


Pro Glu Gln Trp Val Asp Phe Arg Ala Leu Val Gly Asp Pro Ser Asp 
            180                 185                 190         


Asn Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu 
        195                 200                 205             


Leu Lys Glu Trp Gly Ser Leu Glu Asn Ile Leu Lys Asn Leu Asp Arg 
    210                 215                 220                 


Val Lys Pro Glu Ser Val Arg Glu Arg Ile Lys Ala His Leu Glu Asp 
225                 230                 235                 240 


Leu Lys Leu Ser Leu Glu Leu Ser Arg Val Arg Ser Asp Leu Pro Leu 
                245                 250                 255     


Glu Val Asp Phe Ala Arg Arg Arg Glu Pro Asp Arg Glu Gly Leu Arg 
            260                 265                 270         


Ala Phe Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             


Leu Leu Glu Ala Pro Ala Pro Leu Glu Glu Ala Pro Trp Pro Pro Pro 
    290                 295                 300                 


Glu Gly Ala Phe Val Gly Phe Val Leu Ser Arg Pro Glu Pro Met Trp 
305                 310                 315                 320 


Ala Glu Leu Lys Ala Leu Ala Ala Cys Lys Glu Gly Arg Val His Arg 
                325                 330                 335     


Ala Lys Asp Pro Leu Ala Gly Leu Lys Asp Leu Lys Glu Val Arg Gly 
            340                 345                 350         


Leu Leu Ala Lys Asp Leu Ala Val Leu Ala Leu Arg Glu Gly Leu Asp 
        355                 360                 365             


Leu Ala Pro Ser Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro 
    370                 375                 380                 


Ser Asn Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp 
385                 390                 395                 400 


Thr Glu Asp Ala Ala His Arg Ala Leu Leu Ala Glu Arg Leu Gln Gln 
                405                 410                 415     


Asn Leu Leu Glu Arg Leu Lys Gly Glu Glu Lys Leu Leu Trp Leu Tyr 
            420                 425                 430         


Gln Glu Val Glu Lys Pro Leu Ser Arg Val Leu Ala His Met Glu Ala 
        435                 440                 445             


Thr Gly Val Arg Leu Asp Val Ala Tyr Leu Lys Ala Leu Ser Leu Glu 
    450                 455                 460                 


Leu Ala Glu Glu Ile Arg Arg Leu Glu Glu Glu Val Phe Arg Leu Ala 
465                 470                 475                 480 


Gly His Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu 
                485                 490                 495     


Phe Asp Glu Leu Arg Leu Pro Ala Leu Gly Lys Thr Gln Lys Thr Gly 
            500                 505                 510         


Lys Arg Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His 
        515                 520                 525             


Pro Ile Val Glu Lys Ile Leu Gln His Arg Glu Leu Thr Lys Leu Lys 
    530                 535                 540                 


Asn Thr Tyr Val Asp Pro Leu Pro Gly Leu Val His Pro Arg Thr Gly 
545                 550                 555                 560 


Arg Leu His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu 
                565                 570                 575     


Ser Ser Ser Gly Pro Asn Leu Gln Asn Ile Pro Ile Arg Thr Pro Leu 
            580                 585                 590         


Gly Gln Arg Ile Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu 
        595                 600                 605             


Val Ala Leu Asp Tyr Ser Gln Met Glu Leu Arg Val Leu Ala His Leu 
    610                 615                 620                 


Ser Gly Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Lys Asp Ile 
625                 630                 635                 640 


His Thr Gln Thr Ala Ser Trp Met Phe Gly Val Ser Pro Glu Ala Val 
                645                 650                 655     


Asp Pro Leu Met Arg Arg Ala Ala Lys Thr Val Asn Phe Gly Val Leu 
            660                 665                 670         


Tyr Gly Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr 
        675                 680                 685             


Glu Glu Ala Val Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys 
    690                 695                 700                 


Val Arg Ala Trp Lys Glu Lys Thr Leu Glu Glu Gly Arg Lys Arg Gly 
705                 710                 715                 720 


Tyr Val Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn 
                725                 730                 735     


Ala Arg Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn 
            740                 745                 750         


Met Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val 
        755                 760                 765             


Lys Leu Phe Pro His Leu Arg Glu Met Gly Ala Arg Met Leu Leu Gln 
    770                 775                 780                 


Val His Asp Glu Leu Leu Leu Glu Ala Pro Gln Ala Arg Ala Glu Glu 
785                 790                 795                 800 


Val Ala Ala Leu Ala Lys Glu Ala Met Glu Lys Ala Tyr Pro Leu Ala 
                805                 810                 815     


Val Pro Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala 
            820                 825                 830         


Lys Gly 
        


