                         SEQUENCE LISTING

<110>  DSM IP ASSETS B.V.
       NOVOZYMES A/S
 
<120>  ANIMAL FEED COMPOSITION AND USE THEREOF

<130>  33229-WO-PCT

<150>  
<151>  2019-09-11

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wild type sequence with N-terminal SPIRR

<400>  1

Ser Pro Ile Arg Arg Arg Ile Pro Gly Phe Asp Ile Ser Gly Trp Gln 
1               5                   10                  15      


Pro Thr Thr Asp Phe Ala Arg Ala Tyr Ala Asn Gly Asp Arg Phe Val 
            20                  25                  30          


Tyr Ile Lys Ala Thr Glu Gly Thr Thr Phe Lys Ser Ser Ala Phe Ser 
        35                  40                  45              


Arg Gln Tyr Thr Gly Ala Thr Gln Asn Gly Phe Ile Arg Gly Ala Tyr 
    50                  55                  60                  


His Phe Ala Gln Pro Ala Ala Ser Ser Gly Ala Ala Gln Ala Arg Tyr 
65                  70                  75                  80  


Phe Ala Ser Asn Gly Gly Gly Trp Ser Lys Asp Gly Ile Thr Leu Pro 
                85                  90                  95      


Gly Ala Leu Asp Ile Glu Tyr Asn Pro Asn Gly Ala Thr Cys Tyr Gly 
            100                 105                 110         


Leu Ser Gln Ser Ala Met Val Asn Trp Ile Glu Asp Phe Val Thr Thr 
        115                 120                 125             


Tyr His Gly Ile Thr Ser Arg Trp Pro Val Ile Tyr Thr Thr Thr Asp 
    130                 135                 140                 


Trp Trp Thr Gln Cys Thr Gly Asn Ser Asn Arg Phe Ala Asn Arg Cys 
145                 150                 155                 160 


Pro Leu Trp Ile Ala Arg Tyr Ala Ser Ser Val Gly Thr Leu Pro Asn 
                165                 170                 175     


Gly Trp Gly Phe Tyr Thr Phe Trp Gln Tyr Asn Asp Lys Tyr Pro Gln 
            180                 185                 190         


Gly Gly Asp Ser Asn Trp Phe Asn Gly Asp Ala Ser Arg Leu Arg Ala 
        195                 200                 205             


Leu Ala Asn Gly Asp 
    210             


<210>  2
<211>  946
<212>  DNA
<213>  Trichophaea saccata


<220>
<221>  CDS
<222>  (1)..(347)

<220>
<221>  sig_peptide
<222>  (1)..(51)

<220>
<221>  mat_peptide
<222>  (52)..(943)

<220>
<221>  CDS
<222>  (401)..(615)

<220>
<221>  CDS
<222>  (668)..(772)

<220>
<221>  CDS
<222>  (825)..(943)

<400>  2
atg cac gct ctc acc ctt ctc acc gca acc ctc ttc ggt ctc gca gcg         48
Met His Ala Leu Thr Leu Leu Thr Ala Thr Leu Phe Gly Leu Ala Ala           
        -15                 -10                 -5                        

gcc tac cca gtg aag acc gac ctt cac tgc cgc tcc tct ccc agc act         96
Ala Tyr Pro Val Lys Thr Asp Leu His Cys Arg Ser Ser Pro Ser Thr           
-1  1               5                   10                  15            

tcc gcc agc atc gtc cgc acc tac tcc agt gga acg gaa gtc cag atc        144
Ser Ala Ser Ile Val Arg Thr Tyr Ser Ser Gly Thr Glu Val Gln Ile           
                20                  25                  30                

cag tgc cag acc acg ggc act tcg gtc caa gga tcc aat gtc tgg gac        192
Gln Cys Gln Thr Thr Gly Thr Ser Val Gln Gly Ser Asn Val Trp Asp           
            35                  40                  45                    

aag acc cag cac ggt tgc tac gtc gca gac tac tac gtc aag acc ggg        240
Lys Thr Gln His Gly Cys Tyr Val Ala Asp Tyr Tyr Val Lys Thr Gly           
        50                  55                  60                        

cat tct ggg att ttc acc acc aag tgc ggt agc agc tcg ggt gga ggt        288
His Ser Gly Ile Phe Thr Thr Lys Cys Gly Ser Ser Ser Gly Gly Gly           
    65                  70                  75                            

tcc tgc aag cct ccc ccg atc aat gct gct act gtc gca ttg atc aag        336
Ser Cys Lys Pro Pro Pro Ile Asn Ala Ala Thr Val Ala Leu Ile Lys           
80                  85                  90                  95            

gag ttt gag gg  gtaagtgaca gctctgagtg aggtggtatg aggattaaga            387
Glu Phe Glu Gly                                                           
                                                                          

ctgacgagga tag a ttc gtt cct aag ccc gcc ccg gat cct att gga ttg       437
                 Phe Val Pro Lys Pro Ala Pro Asp Pro Ile Gly Leu          
                 100                 105                 110              

ccg acc gtg gga tac ggg cat ctt tgc aag act aag ggc tgc aaa gaa        485
Pro Thr Val Gly Tyr Gly His Leu Cys Lys Thr Lys Gly Cys Lys Glu           
            115                 120                 125                   

gtg cct tac agc ttc cct ctc acc cag gag act gcc acc aag ttg ctt        533
Val Pro Tyr Ser Phe Pro Leu Thr Gln Glu Thr Ala Thr Lys Leu Leu           
        130                 135                 140                       

cag agc gat atc aag act ttc acc tct tgc gtt agc aac tac gtc aag        581
Gln Ser Asp Ile Lys Thr Phe Thr Ser Cys Val Ser Asn Tyr Val Lys           
    145                 150                 155                           

gac tct gtt aag ctc aac gat aac cag tac gga g gtgagttcca               625
Asp Ser Val Lys Leu Asn Asp Asn Gln Tyr Gly                               
160                 165                 170                               

gtgtaacagt gaatttattg atgatattct aagtaatttt ag ct  ctg gcg tct         678
                                               Ala Leu Ala Ser            
                                                                          

tgg gct ttc aac gtc ggc tgc gga aac gtc cag act tct tcg ctg atc        726
Trp Ala Phe Asn Val Gly Cys Gly Asn Val Gln Thr Ser Ser Leu Ile           
175                 180                 185                 190           

aag aga ttg aac gct ggg gag aac cct aac act gtc gct gct cag g          772
Lys Arg Leu Asn Ala Gly Glu Asn Pro Asn Thr Val Ala Ala Gln               
                195                 200                 205               

gtaagatatt tatcccggat ttgctcttga cacatggctg aaaaagttgc ag aa  ctc      829
                                                          Glu Leu         
                                                                          

ccc aag tgg aag tac gct ggt gga aag gtt atg cct ggc ttg gtc cgc        877
Pro Lys Trp Lys Tyr Ala Gly Gly Lys Val Met Pro Gly Leu Val Arg           
        210                 215                 220                       

cgc cgc aat gct gag gtc gcg ctc ttc aag aag ccc agc agc gtt cag        925
Arg Arg Asn Ala Glu Val Ala Leu Phe Lys Lys Pro Ser Ser Val Gln           
    225                 230                 235                           

gcc cac cct ccc aag tgc taa                                            946
Ala His Pro Pro Lys Cys                                                   
240                 245                                                   


<210>  3
<211>  262
<212>  PRT
<213>  Trichophaea saccata

<400>  3

Met His Ala Leu Thr Leu Leu Thr Ala Thr Leu Phe Gly Leu Ala Ala 
        -15                 -10                 -5              


Ala Tyr Pro Val Lys Thr Asp Leu His Cys Arg Ser Ser Pro Ser Thr 
-1  1               5                   10                  15  


Ser Ala Ser Ile Val Arg Thr Tyr Ser Ser Gly Thr Glu Val Gln Ile 
                20                  25                  30      


Gln Cys Gln Thr Thr Gly Thr Ser Val Gln Gly Ser Asn Val Trp Asp 
            35                  40                  45          


Lys Thr Gln His Gly Cys Tyr Val Ala Asp Tyr Tyr Val Lys Thr Gly 
        50                  55                  60              


His Ser Gly Ile Phe Thr Thr Lys Cys Gly Ser Ser Ser Gly Gly Gly 
    65                  70                  75                  


Ser Cys Lys Pro Pro Pro Ile Asn Ala Ala Thr Val Ala Leu Ile Lys 
80                  85                  90                  95  


Glu Phe Glu Gly Phe Val Pro Lys Pro Ala Pro Asp Pro Ile Gly Leu 
                100                 105                 110     


Pro Thr Val Gly Tyr Gly His Leu Cys Lys Thr Lys Gly Cys Lys Glu 
            115                 120                 125         


Val Pro Tyr Ser Phe Pro Leu Thr Gln Glu Thr Ala Thr Lys Leu Leu 
        130                 135                 140             


Gln Ser Asp Ile Lys Thr Phe Thr Ser Cys Val Ser Asn Tyr Val Lys 
    145                 150                 155                 


Asp Ser Val Lys Leu Asn Asp Asn Gln Tyr Gly Ala Leu Ala Ser Trp 
160                 165                 170                 175 


Ala Phe Asn Val Gly Cys Gly Asn Val Gln Thr Ser Ser Leu Ile Lys 
                180                 185                 190     


Arg Leu Asn Ala Gly Glu Asn Pro Asn Thr Val Ala Ala Gln Glu Leu 
            195                 200                 205         


Pro Lys Trp Lys Tyr Ala Gly Gly Lys Val Met Pro Gly Leu Val Arg 
        210                 215                 220             


Arg Arg Asn Ala Glu Val Ala Leu Phe Lys Lys Pro Ser Ser Val Gln 
    225                 230                 235                 


Ala His Pro Pro Lys Cys 
240                 245 


<210>  4
<211>  245
<212>  PRT
<213>  Trichophaea saccata


<220>
<221>  mat_peptide
<222>  (1)..(245)

<400>  4

Tyr Pro Val Lys Thr Asp Leu His Cys Arg Ser Ser Pro Ser Thr Ser 
1               5                   10                  15      


Ala Ser Ile Val Arg Thr Tyr Ser Ser Gly Thr Glu Val Gln Ile Gln 
            20                  25                  30          


Cys Gln Thr Thr Gly Thr Ser Val Gln Gly Ser Asn Val Trp Asp Lys 
        35                  40                  45              


Thr Gln His Gly Cys Tyr Val Ala Asp Tyr Tyr Val Lys Thr Gly His 
    50                  55                  60                  


Ser Gly Ile Phe Thr Thr Lys Cys Gly Ser Ser Ser Gly Gly Gly Ser 
65                  70                  75                  80  


Cys Lys Pro Pro Pro Ile Asn Ala Ala Thr Val Ala Leu Ile Lys Glu 
                85                  90                  95      


Phe Glu Gly Phe Val Pro Lys Pro Ala Pro Asp Pro Ile Gly Leu Pro 
            100                 105                 110         


Thr Val Gly Tyr Gly His Leu Cys Lys Thr Lys Gly Cys Lys Glu Val 
        115                 120                 125             


Pro Tyr Ser Phe Pro Leu Thr Gln Glu Thr Ala Thr Lys Leu Leu Gln 
    130                 135                 140                 


Ser Asp Ile Lys Thr Phe Thr Ser Cys Val Ser Asn Tyr Val Lys Asp 
145                 150                 155                 160 


Ser Val Lys Leu Asn Asp Asn Gln Tyr Gly Ala Leu Ala Ser Trp Ala 
                165                 170                 175     


Phe Asn Val Gly Cys Gly Asn Val Gln Thr Ser Ser Leu Ile Lys Arg 
            180                 185                 190         


Leu Asn Ala Gly Glu Asn Pro Asn Thr Val Ala Ala Gln Glu Leu Pro 
        195                 200                 205             


Lys Trp Lys Tyr Ala Gly Gly Lys Val Met Pro Gly Leu Val Arg Arg 
    210                 215                 220                 


Arg Asn Ala Glu Val Ala Leu Phe Lys Lys Pro Ser Ser Val Gln Ala 
225                 230                 235                 240 


His Pro Pro Lys Cys 
                245 


<210>  5
<211>  129
<212>  PRT
<213>  Gallus gallus

<400>  5

Lys Val Phe Gly Arg Cys Glu Leu Ala Ala Ala Met Lys Arg His Gly 
1               5                   10                  15      


Leu Asp Asn Tyr Arg Gly Tyr Ser Leu Gly Asn Trp Val Cys Ala Ala 
            20                  25                  30          


Lys Phe Glu Ser Asn Phe Asn Thr Gln Ala Thr Asn Arg Asn Thr Asp 
        35                  40                  45              


Gly Ser Thr Asp Tyr Gly Ile Leu Gln Ile Asn Ser Arg Trp Trp Cys 
    50                  55                  60                  


Asn Asp Gly Arg Thr Pro Gly Ser Arg Asn Leu Cys Asn Ile Pro Cys 
65                  70                  75                  80  


Ser Ala Leu Leu Ser Ser Asp Ile Thr Ala Ser Val Asn Cys Ala Lys 
                85                  90                  95      


Lys Ile Val Ser Asp Gly Asn Gly Met Asn Ala Trp Val Ala Trp Arg 
            100                 105                 110         


Asn Arg Cys Lys Gly Thr Asp Val Gln Ala Trp Ile Arg Gly Cys Arg 
        115                 120                 125             


Leu 
    


<210>  6
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F-80470

<400>  6
acacaactgg ggatccacca tgcacgctct cacccttct                              39


<210>  7
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer R-80470.

<400>  7
ctagatctcg agaagctttt agcacttggg agggtggg                               38


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 8643.

<400>  8
gcaagggatg ccatgcttgg                                                   20


<210>  9
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 8654.

<400>  9
catataacca attgccctc                                                    19


<210>  10
<211>  213
<212>  PRT
<213>  Acremonium alcalophilum

<400>  10

Ser Pro Ile Arg Arg Arg Ile Pro Gly Phe Asp Ile Ser Gly Trp Gln 
1               5                   10                  15      


Pro Thr Thr Asp Phe Ala Arg Ala Tyr Ala Asn Gly Asp Arg Phe Val 
            20                  25                  30          


Tyr Ile Lys Ala Thr Glu Gly Thr Thr Phe Lys Ser Ser Ala Phe Ser 
        35                  40                  45              


Arg Gln Tyr Thr Gly Ala Thr Gln Asn Gly Phe Ile Arg Gly Ala Tyr 
    50                  55                  60                  


His Phe Ala Gln Pro Ala Ala Ser Ser Gly Ala Ala Gln Ala Arg Tyr 
65                  70                  75                  80  


Phe Ala Ser Asn Gly Gly Gly Trp Ser Lys Asp Gly Ile Thr Leu Pro 
                85                  90                  95      


Gly Ala Leu Asp Ile Glu Tyr Asn Pro Asn Gly Ala Thr Cys Tyr Gly 
            100                 105                 110         


Leu Ser Gln Ser Ala Met Val Asn Trp Ile Glu Asp Phe Val Thr Thr 
        115                 120                 125             


Tyr His Gly Ile Thr Ser Arg Trp Pro Val Ile Tyr Thr Thr Thr Asp 
    130                 135                 140                 


Trp Trp Thr Gln Cys Thr Gly Asn Ser Asn Arg Phe Ala Asn Arg Cys 
145                 150                 155                 160 


Pro Leu Trp Ile Ala Arg Tyr Ala Ser Ser Val Gly Thr Leu Pro Asn 
                165                 170                 175     


Gly Trp Gly Phe Tyr Thr Phe Trp Gln Tyr Asn Asp Lys Tyr Pro Gln 
            180                 185                 190         


Gly Gly Asp Ser Asn Trp Phe Asn Gly Asp Ala Ser Arg Leu Arg Ala 
        195                 200                 205             


Leu Ala Asn Gly Asp 
    210             


