                         SEQUENCE LISTING

<110>  FUNDACIN DEL SECTOR PBLICO ESTATAL CENTRO NACIONAL DE 
       INVESTIGACIONES ONCOLGICAS CARLOS III (F.S.P. CNIO)
 
<120>  MODULATION OF TRF1 FOR BRAIN CANCER TREATMENT

<130>  PCT-906

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for RT-qPCR for Cre gene

<400>  1
ttaatccata ttggcagaac gaaaacg                                           27


<210>  2
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for RT-qPCR for Cre gene

<400>  2
caggctaagt gccttctcta ca                                                22


<210>  3
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Taqman probe for Cre gene

<400>  3
cctgcggtgc taacc                                                        15


<210>  4
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for RT-qPCR for Terf1-3-MD gene; used to test for 
       Trf1lox; targeted to a sequence unique to the Trf1 recombined 
       allele

<400>  4
gctatacgaa gttattcgag gtcgat                                            26


<210>  5
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for RT-qPCR for Terf1-3-MD gene: used to test for 
       Trf1lox; targeted to a sequence unique to the Trf1 recombined 
       allele

<400>  5
ggtggcggcc gaagt                                                        15


<210>  6
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Taqman probe for Terf1-3-MD gene: used to test for Trf1lox; 
       targeted to a sequence unique to the Trf1 recombined allele

<400>  6
ctctagaaag tataggaact tc                                                22


<210>  7
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for RT-qPCR for Terf1-3-WT gene; used to test for 
       Trf1+; targeted to sequence at the 3' loxP insertion site

<400>  7
gagacggcgc gaaacc                                                       16


<210>  8
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for RT-PCT for Terf1-3-WT gene: used to test for 
       Trf1+; targeted to sequence at the 3' loxP insertion site

<400>  8
gcgggagcca ggacttc                                                      17


<210>  9
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Taqman probe for Terf1-3-WT gene; used to test for Trf1+; 
       targeted to sequence at the 3' loxP insertion site

<400>  9
ccgcttcctg tttgctg                                                      17


<210>  10
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for RT-qPCR for Tva gene: used to test the 
       presence of the Tva transgene

<400>  10
cacagaggct cccactgt                                                     18


<210>  11
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for RT-qPCR for Tva gene: used to test the 
       presence of the Tva transgene

<400>  11
atgcggccgt gattcct                                                      17


<210>  12
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Taqman probe for Tva gene: used to test the presence of the Tva 
       transgene

<400>  12
ctggacgtgc tctgcc                                                       16


<210>  13
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for RT-qPCR for p16-WT gene: used to test the 
       presence of the p16 allele

<400>  13
cgaggacccc actaccttct                                                   20


<210>  14
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for RT-qPCR for p16-WT gene: used to test the 
       presence of the p16 allele

<400>  14
ccgctcttgg gccaagt                                                      17


<210>  15
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Taqman probe for p16-WT gene: used to test the presence of the 
       p16 allele

<400>  15
caggcatcgc gcacat                                                       16


<210>  16
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for RT-qPCR for p16-KO: used to test the absence 
       of the p16 allele

<400>  16
ctctactttt tcttctgact tttcaggtg                                         29


<210>  17
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for RT-qPCR for p16-KO: used to test the absence 
       of the p16 allele

<400>  17
cccctacccg gtagaattga c                                                 21


<210>  18
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Taqman probe to test the absence of the p16 allele

<400>  18
atgatgatgg gcccccgtc                                                    19


<210>  19
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer to assess Cre mediated Trf1 deletion

<400>  19
atagtgatca aaatgtggtc ctggg                                             25


<210>  20
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer (SA-R1) to assess Cre mediated Trf1 deletion

<400>  20
gcttgccaaa ttgggttgg                                                    19


<210>  21
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for mouse TRF1 gene

<400>  21
gtctctgtgc cgagccttc                                                    19


<210>  22
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for qRT-PCR for mouse TRF1 gene

<400>  22
tcaattggta agctgtaagt ctgtg                                             25


<210>  23
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for qRT-PCR for mouse TBP1 gene

<400>  23
acccttcacc aatgactcct atg                                               23


<210>  24
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for qRT-PCR for mouse TBP1 gene

<400>  24
tgactgcagc aaatcgcttg g                                                 21


<210>  25
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for mouse TRF2 gene

<400>  25
agagccagtg gaaaaaccac                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for qRT-PCR for mouse TRF2 gene

<400>  26
atgatgggga tgccagatta                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for mouse POT1A gene

<400>  27
aaactatgaa gccctcccca                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for qRT-PCR for mouse POT1A gene

<400>  28
cgaagccaga gcagttgatt                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for mouse RAP1 gene

<400>  29
aaggaccgct acctcaagca                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for qRT-PCR for mouse RAP1 gene

<400>  30
tgttgtctgc ctctccattc                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for qRT-PCR for mouse TPP1 gene

<400>  31
acttgtgtca gacggaaccc                                                   20


<210>  32
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for qRT-PCR for mouse TPP1 gene

<400>  32
caaccagtca cctgtatcc                                                    19


<210>  33
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for mouse TIN2 gene

<400>  33
tcggttgctt tgcaccagta t                                                 21


<210>  34
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for qRT-PCR for mouse  TIN2

<400>  34
gcttagcttt aggcagagga c                                                 21


<210>  35
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for human TRF1 gene

<400>  35
ttctaatgaa ggcagcggca                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for qRT-PCR for human TRF1 gene

<400>  36
gttgctgggt tccatgttgc                                                   20


<210>  37
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for human a-TUB gene

<400>  37
agtgaaaaca atctaaccag aaa                                               23


<210>  38
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for qRT-PCR for human a-TUB gene

<400>  38
aggcccgtga agatatg                                                      17


<210>  39
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for human TRF2 gene

<400>  39
gaccttccag cagaagatgc t                                                 21


<210>  40
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  reverse primer for qRT-PCR for human TRF2 gene

<400>  40
gttggaggat tccgtagctg                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for human POT1 gene

<400>  41
tgggtattgt acccctccaa                                                   20


<210>  42
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  reverse primer for qRT-PCR for human POT1 gene

<400>  42
gatgaagcat tccaaccacg g                                                 21


<210>  43
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for human RAP1 gene

<400>  43
cggggaacca cagaataaga                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  reverse primer for qRT-PCR for human RAP1 gene

<400>  44
ctcaggtgtg ggtggatcat                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for human TPP1 gene

<400>  45
cccgcagagt tctatctcca                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  reverse primer for qRT-PCR for human TPP1 gene

<400>  46
ggacagtgat aggcctgcat                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer for qRT-PCR for human TIN2 gene

<400>  47
ggagtttctg cgatctctgc                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  reverse primer for qRT-PCR for human TIN2 gene

<400>  48
gatcccgcac tataggtcca                                                   20


