                         SEQUENCE LISTING
<110>  GROPHIN Pty Ltd
 
<120>  METHOD AND COMPOSITIONS FOR IMPROVING RECOVERY FROM A BRAIN INJURY 

<130>  44413P
<160>  13    
<170>  Patent In version 3.5
<210>  1
<211>  1284
<212>  DNA
<213>  Homo sapiens
<400>  1
atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt       60 ctgggggtgt cccttggagg tgccaaggag gcatgcccca caggcctgta cacacacagc      120 ggtgagtgct gcaaagcctg caacctgggc gagggtgtgg cccagccttg tggagccaac      180 cagaccgtgt gtgagccctg cctggacagc gtgacgttct ccgacgtggt gagcgcgacc      240 gagccgtgca agccgtgcac cgagtgcgtg gggctccaga gcatgtcggc gccgtgcgtg      300 gaggccgacg acgccgtgtg ccgctgcgcc tacggctact accaggatga gacgactggg      360 cgctgcgagg cgtgccgcgt gtgcgaggcg ggctcgggcc tcgtgttctc ctgccaggac      420 aagcagaaca ccgtgtgcga ggagtgcccc gacggcacgt attccgacga ggccaaccac      480 gtggacccgt gcctgccctg caccgtgtgc gaggacaccg agcgccagct ccgcgagtgc      540 acacgctggg ccgacgccga gtgcgaggag atccctggcc gttggattac acggtccaca      600 cccccagagg gctcggacag cacagccccc agcacccagg agcctgaggc acctccagaa      660 caagacctca tagccagcac ggtggcaggt gtggtgacca cagtgatggg cagctcccag      720 cccgtggtga cccgaggcac caccgacaac ctcatccctg tctattgctc catcctggct      780 gctgtggttg tgggccttgt ggcctacata gccttcaaga ggtggaacag ctgcaagcag      840 aacaagcaag gagccaacag ccggccagtg aaccagacgc ccccaccaga gggagaaaaa      900 ctccacagcg acagtggcat ctccgtggac agccagagcc tgcatgacca gcagccccac      960 acgcagacag cctcgggcca ggccctcaag ggtgacggag gcctctacag cagcctgccc     1020 ccagccaagc gggaggaggt ggagaagctt ctcaacggct ctgcggggga cacctggcgg     1080 cacctggcgg gcgagctggg ctaccagccc gagcacatag actcctttac ccatgaggcc     1140 tgccccgttc gcgccctgct tgcaagctgg gccacccagg acagcgccac actggacgcc     1200 ctcctggccg ccctgcgccg catccagcga gccgacctcg tggagagtct gtgcagtgag     1260 tccactgcca catccccggt gtga                                            1284
<210>  2
<211>  420
<212>  PRT
<213>  Homo sapiens
<400>  2
Met Gly Ala Gly Ala Thr Gly Arg Ala Met Asp Gly Pro Arg Leu Leu 
1               5                   10                  15      
Leu Leu Leu Leu Leu Gly Val Ser Leu Gly Gly Ala Lys Glu Ala Cys 
            20                  25                  30          
Pro Thr Gly Leu Tyr Thr His Ser Gly Glu Cys Cys Lys Ala Cys Asn 
        35                  40                  45              
Leu Gly Glu Gly Val Ala Gln Pro Cys Gly Ala Asn Gln Thr Val Cys 
    50                  55                  60                  
Glu Pro Cys Leu Asp Ser Val Thr Phe Ser Asp Val Val Ser Ala Thr 
65                  70                  75                  80  
Glu Pro Cys Lys Pro Cys Thr Glu Cys Val Gly Leu Gln Ser Met Ser 
                85                  90                  95      
Ala Pro Cys Val Glu Ala Asp Asp Ala Val Cys Arg Cys Ala Tyr Gly 
            100                 105                 110         
Tyr Tyr Gln Asp Glu Thr Thr Gly Arg Cys Glu Ala Cys Arg Val Cys 
        115                 120                 125             
Glu Ala Gly Ser Gly Leu Val Phe Ser Cys Gln Asp Lys Gln Asn Thr 
    130                 135                 140                 
Val Cys Glu Glu Cys Pro Asp Gly Thr Tyr Ser Asp Glu Ala Asn His 
145                 150                 155                 160 
Val Asp Pro Cys Leu Pro Cys Thr Val Cys Glu Asp Thr Glu Arg Gln 
                165                 170                 175     
Leu Arg Glu Cys Thr Arg Trp Ala Asp Ala Glu Cys Glu Glu Ile Pro 
            180                 185                 190         
Gly Arg Trp Ile Thr Arg Ser Thr Pro Pro Glu Gly Ser Asp Ser Thr 
        195                 200                 205             
Ala Pro Ser Thr Gln Glu Pro Glu Ala Pro Pro Glu Gln Asp Leu Ile 
    210                 215                 220                 
Ala Ser Thr Val Ala Gly Val Val Thr Thr Val Met Gly Ser Ser Gln 
225                 230                 235                 240 
Pro Val Val Thr Arg Gly Thr Thr Asp Asn Leu Ile Pro Val Tyr Cys 
                245                 250                 255     
Ser Ile Leu Ala Ala Val Val Val Gly Leu Val Ala Tyr Ile Ala Phe 
            260                 265                 270         Lys Arg Trp Asn Ser Cys Lys Gln Asn Lys Gln Gly Ala Asn Ser Arg 
        275                 280                 285             
Pro Val Asn Gln Thr Pro Pro Pro Glu Gly Glu Lys Leu His Ser Asp 
    290                 295                 300                 
Ser Gly Ile Ser Val Asp Ser Gln Ser Leu His Asp Gln Gln Pro His 
305                 310                 315                 320 
Thr Gln Thr Ala Ser Gly Gln Ala Leu Lys Gly Asp Gly Gly Leu Tyr 
                325                 330                 335     
Ser Ser Leu Pro Pro Ala Lys Arg Glu Glu Val Glu Lys Leu Leu Asn 
            340                 345                 350         
Gly Ser Ala Gly Asp Thr Trp Arg His Leu Ala Gly Glu Leu Gly Tyr 
        355                 360                 365             
Gln Pro Glu His Ile Asp Ser Phe Thr His Glu Ala Cys Pro Val Arg 
    370                 375                 380                 
Ala Leu Leu Ala Ser Trp Ala Thr Gln Asp Ser Ala Thr Leu Asp Ala 
385                 390                 395                 400 
Leu Leu Ala Ala Leu Arg Arg Ile Gln Arg Ala Asp Leu Val Glu Ser 
                405                 410                 415     
Leu Cys Ser Glu 
            420 
<210>  3
<211>  1188
<212>  DNA
<213>  Homo sapiens
<400>  3
aaggaggcat gccccacagg cctgtacaca cacagcggtg agtgctgcaa agcctgcaac       60 ctgggcgagg gtgtggccca gccttgtgga gccaaccaga ccgtgtgtga gccctgcctg      120 gacagcgtga cgttctccga cgtggtgagc gcgaccgagc cgtgcaagcc gtgcaccgag      180 tgcgtggggc tccagagcat gtcggcgccg tgcgtggagg ccgacgacgc cgtgtgccgc      240 tgcgcctacg gctactacca ggatgagacg actgggcgct gcgaggcgtg ccgcgtgtgc      300 gaggcgggct cgggcctcgt gttctcctgc caggacaagc agaacaccgt gtgcgaggag      360 tgccccgacg gcacgtattc cgacgaggcc aaccacgtgg acccgtgcct gccctgcacc      420 gtgtgcgagg acaccgagcg ccagctccgc gagtgcacac gctgggccga cgccgagtgc      480 gaggagatcc ctggccgttg gattacagac aaaactcaca catgcccacc gtgcccagca      540 cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc      600 atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct      660 gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg      720 cgggaggagc agtacaacag cacgtaccgg gtggtcagcg tcctcaccgt cctgcaccag      780 gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc      840 atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg      900 cccccatccc gggatgagct gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc      960 ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac     1020 aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag caagctcacc     1080 gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggct     1140 ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaa                  1188
<210>  4
<211>  221
<212>  PRT
<213>  Homo sapiens
<400>  4
Glu Ala Cys Pro Thr Gly Leu Tyr Thr His Ser Gly Glu Cys Cys Lys 
1               5                   10                  15      
Ala Cys Asn Leu Gly Glu Gly Val Ala Gln Pro Cys Gly Ala Asn Gln 
            20                  25                  30          
Thr Val Cys Glu Pro Cys Leu Asp Ser Val Thr Phe Ser Asp Val Val 
        35                  40                  45              
Ser Ala Thr Glu Pro Cys Lys Pro Cys Thr Glu Cys Val Gly Leu Gln 
    50                  55                  60                  
Ser Met Ser Ala Pro Cys Val Glu Ala Asp Asp Ala Val Cys Arg Cys 
65                  70                  75                  80  
Ala Tyr Gly Tyr Tyr Gln Asp Glu Thr Thr Gly Arg Cys Glu Ala Cys 
                85                  90                  95      
Arg Val Cys Glu Ala Gly Ser Gly Leu Val Phe Ser Cys Gln Asp Lys 
            100                 105                 110         
Gln Asn Thr Val Cys Glu Glu Cys Pro Asp Gly Thr Tyr Ser Asp Glu 
        115                 120                 125             
Ala Asn His Val Asp Pro Cys Leu Pro Cys Thr Val Cys Glu Asp Thr 
    130                 135                 140                 
Glu Arg Gln Leu Arg Glu Cys Thr Arg Trp Ala Asp Ala Glu Cys Glu 
145                 150                 155                 160 
Glu Ile Pro Gly Arg Trp Ile Thr Arg Ser Thr Pro Pro Glu Gly Ser 
                165                 170                 175     
Asp Ser Thr Ala Pro Ser Thr Gln Glu Pro Glu Ala Pro Pro Glu Gln 
            180                 185                 190         
Asp Leu Ile Ala Ser Thr Val Ala Gly Val Val Thr Thr Val Met Gly 
        195                 200                 205             
Ser Ser Gln Pro Val Val Thr Arg Gly Thr Thr Asp Asn 
    210                 215                 220     
<210>  5
<211>  1188
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  human p75ECD - linker - human Fc fusion
<400>  5
aaggaggcat gccccacagg cctgtacaca cacagcggtg agtgctgcaa agcctgcaac       60 ctgggcgagg gtgtggccca gccttgtgga gccaaccaga ccgtgtgtga gccctgcctg      120 gacagcgtga cgttctccga cgtggtgagc gcgaccgagc cgtgcaagcc gtgcaccgag      180 tgcgtggggc tccagagcat gtcggcgccg tgcgtggagg ccgacgacgc cgtgtgccgc      240 tgcgcctacg gctactacca ggatgagacg actgggcgct gcgaggcgtg ccgcgtgtgc      300 gaggcgggct cgggcctcgt gttctcctgc caggacaagc agaacaccgt gtgcgaggag      360 tgccccgacg gcacgtattc cgacgaggcc aaccacgtgg acccgtgcct gccctgcacc      420 gtgtgcgagg acaccgagcg ccagctccgc gagtgcacac gctgggccga cgccgagtgc      480 gaggagatcc ctggccgttg gattacagac aaaactcaca catgcccacc gtgcccagca      540 cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc      600 atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct      660 gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg      720 cgggaggagc agtacaacag cacgtaccgg gtggtcagcg tcctcaccgt cctgcaccag      780 gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc      840 atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg      900 cccccatccc gggatgagct gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc      960 ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac     1020 aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag caagctcacc     1080 gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggct     1140 ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaa                  1188
<210>  6
<211>  396
<212>  PRT
<213>  Artificial Sequence
<220>
<223>  human p75ECD - linker - human Fc fusion
<400>  6
Lys Glu Ala Cys Pro Thr Gly Leu Tyr Thr His Ser Gly Glu Cys Cys 
1               5                   10                  15      
Lys Ala Cys Asn Leu Gly Glu Gly Val Ala Gln Pro Cys Gly Ala Asn 
            20                  25                  30          
Gln Thr Val Cys Glu Pro Cys Leu Asp Ser Val Thr Phe Ser Asp Val 
        35                  40                  45              
Val Ser Ala Thr Glu Pro Cys Lys Pro Cys Thr Glu Cys Val Gly Leu 
    50                  55                  60                  
Gln Ser Met Ser Ala Pro Cys Val Glu Ala Asp Asp Ala Val Cys Arg 
65                  70                  75                  80  
Cys Ala Tyr Gly Tyr Tyr Gln Asp Glu Thr Thr Gly Arg Cys Glu Ala 
                85                  90                  95      
Cys Arg Val Cys Glu Ala Gly Ser Gly Leu Val Phe Ser Cys Gln Asp 
            100                 105                 110         
Lys Gln Asn Thr Val Cys Glu Glu Cys Pro Asp Gly Thr Tyr Ser Asp 
        115                 120                 125             
Glu Ala Asn His Val Asp Pro Cys Leu Pro Cys Thr Val Cys Glu Asp 
    130                 135                 140                 
Thr Glu Arg Gln Leu Arg Glu Cys Thr Arg Trp Ala Asp Ala Glu Cys 
145                 150                 155                 160 
Glu Glu Ile Pro Gly Arg Trp Ile Thr Asp Lys Thr His Thr Cys Pro 
                165                 170                 175     
Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
            180                 185                 190         
Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
        195                 200                 205             
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
    210                 215                 220                 
Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
225                 230                 235                 240 Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
                245                 250                 255     
Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
            260                 265                 270         
Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
        275                 280                 285             
Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
    290                 295                 300                 
Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
305                 310                 315                 320 
Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
                325                 330                 335     
Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
            340                 345                 350         
Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
        355                 360                 365             
Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
    370                 375                 380                 
Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
385                 390                 395     
<210>  7
<211>  35
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Forward PCR primer for human p75ECD
<400>  7
gcgctctaga ccaccatggg ggcaggtgcc accgg                                  35
<210>  8
<211>  33
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Reverse PCR primer for human p75ECD
<400>  8
ctctgtcgac ttatcattta cccggagaca ggg                                    33
<210>  9
<211>  27
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Forward PCR primer for human Fc
<400>  9
aaccatgggc gacaaaactc acacatg                                           27
<210>  10
<211>  26
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Reverse PCR primer for human Fc
<400>  10
aagaattctc atttacccgg agacag                                            26
<210>  11
<211>  30
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Forward PCR primer for human Fc
<400>  11
ttggattaca gacaaaactc acacatgccc                                        30
<210>  12
<211>  33
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Reverse PCR primer for human Fc
<400>  12
ctctgtcgac ttatcattta cccggagaca ggg                                    33
<210>  13
<211>  2217
<212>  DNA
<213>  Artificial Sequence
<220>
<223>  Adeno-associated virus (AAV)8 capsid sequence with an AAV2        phospholipase domain
<400>  13
atggctgccg atggttatct tccagattgg ctcgaggaca ctctctctga aggaataaga       60 cagtggtgga agctcaaacc tggcccacca ccaccaaagc ccgcagagcg gcataaggac      120 gacagcaggg gtcttgtgct tcctgggtac aagtacctcg gacccttcaa cggactcgac      180 aagggagagc cggtcaacga ggcagacgcc gcggccctcg agcacgacaa agcctacgac      240 cggcagctcg acagcggaga caacccgtac ctcaagtaca accacgccga cgcggagttt      300 caggagcgcc ttaaagaaga tacgtctttt gggggcaacc tcggacgagc agtcttccag      360 gcgaaaaaga gggttcttga acctctgggc ctggttgagg aacctgttaa gacggctccg      420 ggaaagaaga gaccggtaga gccatcaccc cagcgttctc cagactcctc tacgggcatc      480 ggcaagaaag gccaacagcc cgccagaaaa agactcaatt ttggtcagac tggcgactca      540 gagtcagttc cagaccctca acctctcgga gaacctccag cagcgccctc tggtgtggga      600 cctaatacaa tggctgcagg cggtggcgca ccaatggcag acaataacga aggcgccgac      660 ggagtgggta gttcctcggg aaattggcat tgcgattcca catggctggg cgacagagtc      720 atcaccacca gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaagcaa      780 atctccaacg ggacatcggg aggagccacc aacgacaaca cctacttcgg ctacagcacc      840 ccctgggggt attttgactt taacagattc cactgccact tttcaccacg tgactggcag      900 cgactcatca acaacaactg gggattccgg cccaagagac tcagcttcaa gctcttcaac      960 atccaggtca aggaggtcac gcagaatgaa ggcaccaaga ccatcgccaa taacctcacc     1020 agcaccatcc aggtgtttac ggactcggag taccagctgc cgtacgttct cggctctgcc     1080 caccagggct gcctgcctcc gttcccggcg gacgtgttca tgattcccca gtacggctac     1140 ctaacactca acaacggtag tcaggccgtg ggacgctcct ccttctactg cctggaatac     1200 tttccttcgc agatgctgag aaccggcaac aacttccagt ttacttacac cttcgaggac     1260 gtgcctttcc acagcagcta cgcccacagc cagagcttgg accggctgat gaatcctctg     1320 attgaccagt acctgtacta cttgtctcgg actcaaacaa caggaggcac ggcaaatacg     1380 cagactctgg gcttcagcca aggtgggcct aatacaatgg ccaatcaggc aaagaactgg     1440 ctgccaggac cctgttaccg ccaacaacgc gtctcaacga caaccgggca aaacaacaat     1500 agcaactttg cctggactgc tgggaccaaa taccatctga atggaagaaa ttcattggct     1560 aatcctggca tcgctatggc aacacacaaa gacgacgagg agcgtttttt tcccagtaac     1620 gggatcctga tttttggcaa acaaaatgct gccagagaca atgcggatta cagcgatgtc     1680 atgctcacca gcgaggaaga aatcaaaacc actaaccctg tggctacaga ggaatacggt     1740 atcgtggcag ataacttgca gcagcaaaac acggctcctc aaattggaac tgtcaacagc     1800 cagggggcct tacccggtat ggtctggcag aaccgggacg tgtacctgca gggtcccatc     1860 tgggccaaga ttcctcacac ggacggcaac ttccacccgt ctccgctgat gggcggcttt     1920 ggcctgaaac atcctccgcc tcagatcctg atcaagaaca cgcctgtacc tgcggatcct     1980 ccgaccacct tcaaccagtc aaagctgaac tctttcatca cgcaatacag caccggacag     2040 gtcagcgtgg aaattgaatg ggagctgcag aaggaaaaca gcaagcgctg gaaccccgag     2100 atccagtaca cctccaacta ctacaaatct acaagtgtgg actttgctgt taatacagaa     2160 ggcgtgtact ctgaaccccg ccccattggc acccgttacc tcacccgtaa tctgtaa        2217
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