                         SEQUENCE LISTING

<110>  BLUEALLELE, LLC
 
<120>  METHODS AND COMPOSITIONS FOR MODIFYING THE VON WILLEBRAND FACTOR 
       GENE

<130>  BA2018-2WO

<150>  62/728,760
<151>  2018-09-08

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  4636
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct

<400>  1
ggcactctgt agcttttagg gtgaggatat actctagcga gtccatggca catggcttag       60

ggaggacgcc tgccttctct tcacccactt cactccatat ctctgtatcc tcctgggaac      120

tcgagagcag cctggccttc ctttctcctg cagactctgt gctttgaggc ccatgtgcct      180

tccacaggct ccaaatgctt ggcttcttga ggctacctac gtgatgacct ggcatcttga      240

actcaatctg tagaccaggc tggccttgaa ctcagaaatt cacctgcctc tgcctgccaa      300

gtgctggtgt gtgtcaccac gcccggcttt ttatttattt tattattatt attattatta      360

ttattattat tattatttgg tttgcctcct tgtttcctaa ggcctgattg aacccctggt      420

gcctaaggta caaaccagta acacttgatt gctgtcccta gtgtctgccg ggagcggaag      480

tggaactgca cgaaccatgt gtgtgacgcc acttgctctg ccattggtat ggcccactac      540

ctcaccttcg atggactcaa gtacctgttc ccgggggagt gccagtatgt tctggtgcag      600

agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac      660

ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa      720

tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt      780

atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc      840

ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat      900

gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgctgatgc      960

ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc     1020

tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa     1080

aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg     1140

tctatataag cagagctggt ttagtgaacc gtcagatcag atctttgtcg atcctaccat     1200

ccactcgaca cacccgccag cggccgcgtt ggtatcaagg ttacaagaca ggtttaagga     1260

gaccaataga aactgggcat gtggagacag agaagactct tgggtttctg ataggcactg     1320

actctcttcc tttgtcctgt tcccatttca gatgaaccct ttcaggtatg agatctgcct     1380

gcttgttctg gccctcacct ggccagggac cctctgcaca gaaaagcccc gtgacaggcc     1440

gtcgacggcc cgatgcagcc tctttgggga cgacttcatc aacacgtttg atgagaccat     1500

gtacagcttt gcagggggct gcagttatct cctggctggg gactgccaga aacgttcctt     1560

ctccattctc gggaacttcc aagatggcaa gagaatgagc ctgtctgtgt atcttgggga     1620

gttttttgac atccatttgt ttgccaatgg caccgtaacg cagggtgacc aaagcatctc     1680

catgccctac gcctcccaag gactctacct agaacgcgag gctgggtact ataagctctc     1740

cagtgagacc tttggctttg cggccagaat cgatggcaat ggcaacttcc aagtcctgat     1800

gtcagacaga cacttcaaca agacctgtgg gctgtgcggt gattttaaca tcttcgcgga     1860

agatgatttt aggacgcagg aggggacctt gacctcagac ccctatgatt ttgccaactc     1920

ctgggccctg agcagtgagg aacagcggtg taaacgggca tctcctccca gcaggaactg     1980

cgagagctct tctggggaca tgcatcaggc catgtgggag caatgccagc tactgaagac     2040

ggcatcggtg tttgcccgct gccaccctct ggtggatccc gagtcctttg tggctctgtg     2100

tgagaagatt ttgtgtacgt gtgctacggg gccagagtgc gcatgtcctg tactccttga     2160

gtatgcccga acctgcgccc aggaagggat ggtgctgtac ggctggactg accacagtgc     2220

ctgtcgtcca gcttgcccag ctggcatgga atataaggag tgtgtgtctc cttgccccag     2280

aacctgccag agcctgtcta tcaatgaagt gtgtcagcag caatgtgtag acggctgtag     2340

ctgccctgag ggagagctct tggatgaaga ccgatgtgtg cagagctccg actgtccttg     2400

cgtgcacgct gggaagcggt accctcctgg cacctccctc tctcaggact gcaacacttg     2460

tatctgcaga aacagcctat ggatctgcag caatgaggaa tgcccagggg agtgtcttgt     2520

cacaggccaa tcgcacttca agagcttcga caacaggtac ttcaccttca gtgggatctg     2580

ccaatatctg ctggcccggg actgcgagga tcacactttc tccattgtca tagagaccat     2640

gcagtgtgcc gatgaccctg atgctgtctg cacccgctcg gtcagtgtgc ggctctctgc     2700

cctgcacaac agcctggtga aactgaagca cgggggagca gtgggcatcg atggtcagga     2760

tgtccagctc cccttcctgc aaggtgacct ccgcatccag cacacagtga tggcttctgt     2820

acgcctcagc tatgcggagg acctgcagat ggactgggat ggccgtgggc ggctactggt     2880

taagctgtcc ccagtctatt ctgggaagac ctgtggcttg tgtgggaatt acaacggcaa     2940

caagggagac gacttcctca cgccggccgg cttggtggag cccctggtgg tagacttcgg     3000

aaacgcctgg aagcttcaag gggactgttc ggacctgcgc aggcaacaca gcgacccctg     3060

cagcctgaat ccacgcttga ccaggtttgc agaggaggct tgtgcgctcc tgacgtcctc     3120

caagttcgag gcctgccacc acgcagtcag ccctctgccc tatctgcaga actgccgtta     3180

tgatgtttgc tcctgctccg acagccggga ttgcctgtgt aacgcagtag ctaactatgc     3240

tgccgagtgt gcccgaaaag gcgtgcacat cgggtggcgg gagcctggct tctgtgctct     3300

gggctgtcca cagggccagg tgtacctgca gtgtgggaat tcctgcaacc tgacctgccg     3360

ctccctctcc ctcccggatg aagaatgcag tgaagtctgt cttgaaggct gctactgccc     3420

accagggctc taccaggatg aaagagggga ctgtgtgccc aaggcccagt gcccctgcta     3480

ctacgatggt gagctcttcc agcctgcgga cattttctca gaccaccata ccatgtgtta     3540

ctgtgaagat ggcttcatgc actgtaccac aagtggcacc ctggggagcc tgttgcctga     3600

cactgtcctc agcagtcccc tgtctcaccg tagcaaaagg agcctttcct gccggccacc     3660

catggtcaag ctggtgtgtc ctgctgacaa cccacgggct caagggctgg agtgtgctaa     3720

gacgtgccag aactacgacc tggagtgtat gagcctgggc tgtgtgtctg gctgcctctg     3780

tcccccaggc atggtccggc acgaaaacaa gtgtgtggcc ttggagcggt gtccctgctt     3840

ccatcagggt gcagagtacg ccccgggaga cacagtgaag attggctgca acacctgtgt     3900

ctgccgggag cggaagtgga actgcacgaa ccatgtgtgt gacgccactt gctctgccat     3960

tggtatggcc cactacctca ccttcgatgg actcaagtac ctgttcccgg gggagtgcca     4020

gtatgttctg gtgcaggtga gactggaaat gaagagaggg ggtgttgtct gtgtcgggca     4080

gagcaggggg tgccgtggga gtctctgggt caggttctat ctatagaaag acctttaggg     4140

tttggttttg tcagagtaat ttttttttaa ttcccccaaa ggatgcccaa cgatgtagga     4200

agtttaagaa cagaatttca ttttctgagc tggactggta tggtgctcct caggccattt     4260

tggcacaggt atagattaga gaaagatgac tctccatttt ggtggacatt aaaccagagc     4320

acatgggagc tgatcctgga tgtcaccctc cctgatagcc cattgttagg aagtattcct     4380

gggcaggggg attgcctgca tgacccctgg agtgtgctgc ctgacctatg gtcctattga     4440

agttgtcgcc atttttatcc ttaacatggt gaggctgggg ctgcaggtgc tgctcagtcc     4500

agcaggaaga acatagagca tactatggag ccccgccagc ccctgtgcct cctcagaccc     4560

accttgtggg ctcctgtgat ttcttctgtt ggccgacacc acatagttca ccaaggagga     4620

caagtgcttt tctcag                                                     4636


<210>  2
<211>  4771
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct

<400>  2
atgtacaggt atgttcctgt gtgtgcatgt ttatccgtga gtgtgtgtgt atgtttaggc       60

atatatatta tatgcttgta catgtgtatg tgtgtataca tatgtatgtg tgtatgtatg      120

tatatgcatg tggaagtata tatgaatgtg tgtttgtgtt tgtatatgca tgtgtgccta      180

tctgtgtatg tgtatgttta tatatgtatt tgtatgtgga tacatgtgct gtctcctcta      240

gcacccctgt tttcctttgt gtatgcctct gtgctgagcg tgggtaccaa ggtcatctgt      300

aagggctgag gttagaccct tgagagagtt tccctggggc tacctctccc tttcccccat      360

gtacagggtg catctgatga tattctctga ccctggaagt cacgatgcca tcttctgctc      420

tgtgagaagg ggtaatcgtt ccctcttggt gccctctctc cctaggatta ctgtggcagt      480

aaccctggga cctttcagat cctggtggga aatgagggtt gcagctatcc ctcggtgaag      540

tgcaggaagc gggtgaccat cctggtggat ggaggggagc ttgaactgtt tgacggagag      600

agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac      660

ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa      720

tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt      780

atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc      840

ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat      900

gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgctgatgc      960

ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc     1020

tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa     1080

aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg     1140

tctatataag cagagctggt ttagtgaacc gtcagatcag atctttgtcg atcctaccat     1200

ccactcgaca cacccgccag cggccgcgtt ggtatcaagg ttacaagaca ggtttaagga     1260

gaccaataga aactgggcat gtggagacag agaagactct tgggtttctg ataggcactg     1320

actctcttcc tttgtcctgt tcccatttca gatgaaccct ttcaggtatg agatctgcct     1380

gcttgttctg gccctcacct ggccagggac cctctgcaca gaaaagcccc gtgacaggcc     1440

gtcgacggcc cgatgcagcc tctttgggga cgacttcatc aacacgtttg atgagaccat     1500

gtacagcttt gcagggggct gcagttatct cctggctggg gactgccaga aacgttcctt     1560

ctccattctc gggaacttcc aagatggcaa gagaatgagc ctgtctgtgt atcttgggga     1620

gttttttgac atccatttgt ttgccaatgg caccgtaacg cagggtgacc aaagcatctc     1680

catgccctac gcctcccaag gactctacct agaacgcgag gctgggtact ataagctctc     1740

cagtgagacc tttggctttg cggccagaat cgatggcaat ggcaacttcc aagtcctgat     1800

gtcagacaga cacttcaaca agacctgtgg gctgtgcggt gattttaaca tcttcgcgga     1860

agatgatttt aggacgcagg aggggacctt gacctcagac ccctatgatt ttgccaactc     1920

ctgggccctg agcagtgagg aacagcggtg taaacgggca tctcctccca gcaggaactg     1980

cgagagctct tctggggaca tgcatcaggc catgtgggag caatgccagc tactgaagac     2040

ggcatcggtg tttgcccgct gccaccctct ggtggatccc gagtcctttg tggctctgtg     2100

tgagaagatt ttgtgtacgt gtgctacggg gccagagtgc gcatgtcctg tactccttga     2160

gtatgcccga acctgcgccc aggaagggat ggtgctgtac ggctggactg accacagtgc     2220

ctgtcgtcca gcttgcccag ctggcatgga atataaggag tgtgtgtctc cttgccccag     2280

aacctgccag agcctgtcta tcaatgaagt gtgtcagcag caatgtgtag acggctgtag     2340

ctgccctgag ggagagctct tggatgaaga ccgatgtgtg cagagctccg actgtccttg     2400

cgtgcacgct gggaagcggt accctcctgg cacctccctc tctcaggact gcaacacttg     2460

tatctgcaga aacagcctat ggatctgcag caatgaggaa tgcccagggg agtgtcttgt     2520

cacaggccaa tcgcacttca agagcttcga caacaggtac ttcaccttca gtgggatctg     2580

ccaatatctg ctggcccggg actgcgagga tcacactttc tccattgtca tagagaccat     2640

gcagtgtgcc gatgaccctg atgctgtctg cacccgctcg gtcagtgtgc ggctctctgc     2700

cctgcacaac agcctggtga aactgaagca cgggggagca gtgggcatcg atggtcagga     2760

tgtccagctc cccttcctgc aaggtgacct ccgcatccag cacacagtga tggcttctgt     2820

acgcctcagc tatgcggagg acctgcagat ggactgggat ggccgtgggc ggctactggt     2880

taagctgtcc ccagtctatt ctgggaagac ctgtggcttg tgtgggaatt acaacggcaa     2940

caagggagac gacttcctca cgccggccgg cttggtggag cccctggtgg tagacttcgg     3000

aaacgcctgg aagcttcaag gggactgttc ggacctgcgc aggcaacaca gcgacccctg     3060

cagcctgaat ccacgcttga ccaggtttgc agaggaggct tgtgcgctcc tgacgtcctc     3120

caagttcgag gcctgccacc acgcagtcag ccctctgccc tatctgcaga actgccgtta     3180

tgatgtttgc tcctgctccg acagccggga ttgcctgtgt aacgcagtag ctaactatgc     3240

tgccgagtgt gcccgaaaag gcgtgcacat cgggtggcgg gagcctggct tctgtgctct     3300

gggctgtcca cagggccagg tgtacctgca gtgtgggaat tcctgcaacc tgacctgccg     3360

ctccctctcc ctcccggatg aagaatgcag tgaagtctgt cttgaaggct gctactgccc     3420

accagggctc taccaggatg aaagagggga ctgtgtgccc aaggcccagt gcccctgcta     3480

ctacgatggt gagctcttcc agcctgcgga cattttctca gaccaccata ccatgtgtta     3540

ctgtgaagat ggcttcatgc actgtaccac aagtggcacc ctggggagcc tgttgcctga     3600

cactgtcctc agcagtcccc tgtctcaccg tagcaaaagg agcctttcct gccggccacc     3660

catggtcaag ctggtgtgtc ctgctgacaa cccacgggct caagggctgg agtgtgctaa     3720

gacgtgccag aactacgacc tggagtgtat gagcctgggc tgtgtgtctg gctgcctctg     3780

tcccccaggc atggtccggc acgaaaacaa gtgtgtggcc ttggagcggt gtccctgctt     3840

ccatcagggt gcagagtacg ccccgggaga cacagtgaag attggctgca acacctgtgt     3900

ctgccgggag cggaagtgga actgcacgaa ccatgtgtgt gacgccactt gctctgccat     3960

tggtatggcc cactacctca ccttcgatgg actcaagtac ctgttcccgg gggagtgcca     4020

gtatgttctg gtgcaggatt actgtggcag taaccctggg acctttcaga tcctggtggg     4080

aaatgagggt tgcagctatc cctcggtgaa gtgcaggaag cgggtgacca tcctggtgga     4140

tggaggggag cttgaactgt ttgacggaga ggtaagtgcc agtctctccc ctttaccttt     4200

atgtcccctt ttgtccctcc atttattagg agcggtttcc caatgttcat ttagaactga     4260

gctggtagaa tggcagccat tgtagagaat gaggttgagc tgtgttagct ggtcttgaga     4320

gtagaacaat tgacagacca atacaggcca tgtgagccca ttgcggttca agtgtgggtg     4380

tgtgtgtggg agagggtctc ctacagtgat ctctgcctgt gtatcctcat acagtgatga     4440

ctggtggagg tgagcagagt gtggcaggca ggaggggttt cccttccatg tacagtggtc     4500

cccctccctt ttagccacag agggataaaa cccctgggag atgcctgaga tcacaggcaa     4560

tacagaaccc agtgtgttcc gttatgcata ttggtaatgt cttagggctt ctattacagt     4620

gacgaaacac catgaccaaa aagcaagtgg gggaggaagt ggtttatttg gcttacactt     4680

ccacatcact gttcatcatc aaaggaagtc aggacaggaa ctcaaacagg acaggaacct     4740

ggaggcagga gctgatgcag aagccatcaa g                                    4771


<210>  3
<211>  4918
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct

<400>  3
atggtagtca cttgggagaa cagaggatgg caggaaaaag gaaaacaagt cgtgggggtg       60

ggtcataggt gggtagagtt tttatcccat tgactagagg tggttaaaat catggccaca      120

ttaaaaagca agtcagggac tggtgtagtt ggtgatagag ggtcaaggca tcctccctcc      180

cttcttgtga ggtgacagga cgtgtgctgg tctctttctc tgtgttctca ctgtggtctt      240

gtcatcatag gagggtgcta ggtcagacag atgcagccat tcctgtggag cggagggctg      300

gcctcaggga gttctgctag aaagtagctg gtggcacaca caggtctcaa gggccatctc      360

tgctattggt gggcaaggca gacaggctgt gtgaaaaggg agccctggac tctgccctcc      420

ttcacctcat tttcccgttt cttcctcctt caggtgaacg ttaagaggcc cctgagagat      480

gaatctcact ttgaggtggt ggagtcgggc cggtacgtca tcctgctgct gggtcaggcc      540

ctttctgtgg tctgggacca ccacctcagc atctctgtgg tcctgaagca cacataccag      600

agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac      660

ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa      720

tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt      780

atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc      840

ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat      900

gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgctgatgc      960

ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc     1020

tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa     1080

aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg     1140

tctatataag cagagctggt ttagtgaacc gtcagatcag atctttgtcg atcctaccat     1200

ccactcgaca cacccgccag cggccgcgtt ggtatcaagg ttacaagaca ggtttaagga     1260

gaccaataga aactgggcat gtggagacag agaagactct tgggtttctg ataggcactg     1320

actctcttcc tttgtcctgt tcccatttca gatgaaccct ttcaggtatg agatctgcct     1380

gcttgttctg gccctcacct ggccagggac cctctgcaca gaaaagcccc gtgacaggcc     1440

gtcgacggcc cgatgcagcc tctttgggga cgacttcatc aacacgtttg atgagaccat     1500

gtacagcttt gcagggggct gcagttatct cctggctggg gactgccaga aacgttcctt     1560

ctccattctc gggaacttcc aagatggcaa gagaatgagc ctgtctgtgt atcttgggga     1620

gttttttgac atccatttgt ttgccaatgg caccgtaacg cagggtgacc aaagcatctc     1680

catgccctac gcctcccaag gactctacct agaacgcgag gctgggtact ataagctctc     1740

cagtgagacc tttggctttg cggccagaat cgatggcaat ggcaacttcc aagtcctgat     1800

gtcagacaga cacttcaaca agacctgtgg gctgtgcggt gattttaaca tcttcgcgga     1860

agatgatttt aggacgcagg aggggacctt gacctcagac ccctatgatt ttgccaactc     1920

ctgggccctg agcagtgagg aacagcggtg taaacgggca tctcctccca gcaggaactg     1980

cgagagctct tctggggaca tgcatcaggc catgtgggag caatgccagc tactgaagac     2040

ggcatcggtg tttgcccgct gccaccctct ggtggatccc gagtcctttg tggctctgtg     2100

tgagaagatt ttgtgtacgt gtgctacggg gccagagtgc gcatgtcctg tactccttga     2160

gtatgcccga acctgcgccc aggaagggat ggtgctgtac ggctggactg accacagtgc     2220

ctgtcgtcca gcttgcccag ctggcatgga atataaggag tgtgtgtctc cttgccccag     2280

aacctgccag agcctgtcta tcaatgaagt gtgtcagcag caatgtgtag acggctgtag     2340

ctgccctgag ggagagctct tggatgaaga ccgatgtgtg cagagctccg actgtccttg     2400

cgtgcacgct gggaagcggt accctcctgg cacctccctc tctcaggact gcaacacttg     2460

tatctgcaga aacagcctat ggatctgcag caatgaggaa tgcccagggg agtgtcttgt     2520

cacaggccaa tcgcacttca agagcttcga caacaggtac ttcaccttca gtgggatctg     2580

ccaatatctg ctggcccggg actgcgagga tcacactttc tccattgtca tagagaccat     2640

gcagtgtgcc gatgaccctg atgctgtctg cacccgctcg gtcagtgtgc ggctctctgc     2700

cctgcacaac agcctggtga aactgaagca cgggggagca gtgggcatcg atggtcagga     2760

tgtccagctc cccttcctgc aaggtgacct ccgcatccag cacacagtga tggcttctgt     2820

acgcctcagc tatgcggagg acctgcagat ggactgggat ggccgtgggc ggctactggt     2880

taagctgtcc ccagtctatt ctgggaagac ctgtggcttg tgtgggaatt acaacggcaa     2940

caagggagac gacttcctca cgccggccgg cttggtggag cccctggtgg tagacttcgg     3000

aaacgcctgg aagcttcaag gggactgttc ggacctgcgc aggcaacaca gcgacccctg     3060

cagcctgaat ccacgcttga ccaggtttgc agaggaggct tgtgcgctcc tgacgtcctc     3120

caagttcgag gcctgccacc acgcagtcag ccctctgccc tatctgcaga actgccgtta     3180

tgatgtttgc tcctgctccg acagccggga ttgcctgtgt aacgcagtag ctaactatgc     3240

tgccgagtgt gcccgaaaag gcgtgcacat cgggtggcgg gagcctggct tctgtgctct     3300

gggctgtcca cagggccagg tgtacctgca gtgtgggaat tcctgcaacc tgacctgccg     3360

ctccctctcc ctcccggatg aagaatgcag tgaagtctgt cttgaaggct gctactgccc     3420

accagggctc taccaggatg aaagagggga ctgtgtgccc aaggcccagt gcccctgcta     3480

ctacgatggt gagctcttcc agcctgcgga cattttctca gaccaccata ccatgtgtta     3540

ctgtgaagat ggcttcatgc actgtaccac aagtggcacc ctggggagcc tgttgcctga     3600

cactgtcctc agcagtcccc tgtctcaccg tagcaaaagg agcctttcct gccggccacc     3660

catggtcaag ctggtgtgtc ctgctgacaa cccacgggct caagggctgg agtgtgctaa     3720

gacgtgccag aactacgacc tggagtgtat gagcctgggc tgtgtgtctg gctgcctctg     3780

tcccccaggc atggtccggc acgaaaacaa gtgtgtggcc ttggagcggt gtccctgctt     3840

ccatcagggt gcagagtacg ccccgggaga cacagtgaag attggctgca acacctgtgt     3900

ctgccgggag cggaagtgga actgcacgaa ccatgtgtgt gacgccactt gctctgccat     3960

tggtatggcc cactacctca ccttcgatgg actcaagtac ctgttcccgg gggagtgcca     4020

gtatgttctg gtgcaggatt actgtggcag taaccctggg acctttcaga tcctggtggg     4080

aaatgagggt tgcagctatc cctcggtgaa gtgcaggaag cgggtgacca tcctggtgga     4140

tggaggggag cttgaactgt ttgacggaga ggtgaacgtt aagaggcccc tgagagatga     4200

atctcacttt gaggtggtgg agtcgggccg gtacgtcatc ctgctgctgg gtcaggccct     4260

ttctgtggtc tgggaccacc acctcagcat ctctgtggtc ctgaagcaca cataccaggt     4320

tagcaggcct tcttgctgct tcttgcctga ttcctgtgga ctgacatcag ttctctaaga     4380

agtaacctgc tgccctttcc cagtcacatt gggggacagt ggttctctct ctggtctagc     4440

ctccttgctc cccacacaag ggaagctaag tagtcacaga gggtgactgt acgtggggag     4500

gacagagaca gctttgacag tgtcttgact agcccaggca ggcacacatt ttgttttcac     4560

tgaggaggga gagcaaggat aggcagggta gcttttcttt aggtttctaa acccacagag     4620

gcaaattaaa tccacaaaat gttaaatcat tgccatctat tctgggatgt tgttttacca     4680

gtgagcccag gctagcacac atgatcatgc acatgcttgt gtgtgtacat gaatgtgtgc     4740

atgtatgtgt gcatgtagag gccagccagt gggcctcatt tctcaggaga catttacttt     4800

gtgttttgag acaaagtctt tcaccaggac ctgggattgc tcagcagtta ggctgggcta     4860

gctggctagt gagctttgag gacttgtttc tgcctcccca gcattggggt cacatatg       4918


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target site

<400>  4
tgttctggtg caggtgagac                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target site

<400>  5
ggggagcttg aactgtttga                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target site

<400>  6
agcaagaagg cctgctaacc                                                   20


<210>  7
<211>  4811
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct

<400>  7
tacttctctg tgaatgacac aacttccttc tctcccaggc actgatgcca cattttcttg       60

actccttcaa aggtcacaca cagcctgttt cccagttaca tgctgtactg tctctttgcc      120

cttgtttcaa ccagtcttag acccaagaac acagaagcag attttctttc tcttattaat      180

tgtttagcta attctcagaa ttatgagtct tagaatgaca ttttcatata tatacatata      240

cacatacaca tacacataca tatacatata tatttttttc ctggccccct tcttccccac      300

aaacaacctc agttactttt cctgttattt agagagggca gcctcttgct ctcatttgca      360

gctatttgca ctgtctgtgg gtagagctcc agtcttttcg atgactgtca atctagtgag      420

cccatagatt caggaactgt ctcctctgtc cttctacctg acccattccc atgccctgcc      480

ctccctggca aacacgtgct cagtggtgca ctgaagacca ctggctgttg tgggggctga      540

cggctggcct tccattagca cctgtgactt gtgtacccat gctcttgttt ctctctgcag      600

actgcagcca acccctggat gtggtcctgc tcctggatgg ctcctctagc ttgccagagt      660

cttcctttga taaaatgaag agttttgcca aggctttcat ttcaaaggcc aacattgggc      720

cccacctcac acaggtgtcc gtgatacagt atggaagcat caataccatt gatgtaccat      780

ggaatgtggt tcaggagaaa gcccatctac agagtttggt ggacctcatg cagcaggagg      840

gtggccccag ccagattggg gatgctctgg cctttgccgt gcgctatgta acttcacaaa      900

tccacggagc caggcctggg gcctccaaag cagtggtcat catcatcatg gatacctcct      960

tggatcccgt ggacacagca gcagatgctg ccagatccaa ccgagtggca gtgtttcccg     1020

ttggggttgg ggatcggtat gatgaagccc agctgaggat cttggcaggc cctggggcca     1080

gctccaatgt ggtaaagctc cagcaagttg aagacctctc caccatggcc accctgggca     1140

actccttctt ccacaaactg tgttctgggt tttctggagt ttgtgtggat gaagatggga     1200

atgagaagag gcctggggat gtctggacct tgccggatca gtgccacaca gtgacttgct     1260

tggcaaatgg ccagaccttg ctgcagagtc atcgtgtcaa ttgtgaccat ggaccccggc     1320

cttcatgtgc caacagccag tctcctgttc gggtggagga gacgtgtggc tgccgctgga     1380

cctgcccttg tgtgtgcacg ggcagttcca ctcggcacat cgtcaccttc gatgggcaga     1440

atttcaagct tactggtagc tgctcctatg tcatctttca aaacaaggag caggacctgg     1500

aagtgctcct ccacaatggg gcctgcagcc ccggggcaaa acaagcctgc atgaagtcca     1560

ttgagattaa gcatgctggc gtctctgctg agctgcacag taacatggag atggcagtgg     1620

atgggagact ggtccttgcc ccgtacgttg gtgaaaacat ggaagtcagc atctacggcg     1680

ctatcatgta tgaagtcagg tttacccatc ttggccacat cctcacatac acgccacaaa     1740

acaacgagtt ccaactgcag cttagcccca agacctttgc ttcgaagatg catggtcttt     1800

gcggaatctg tgatgaaaac ggggccaatg acttcacgtt gcgagatggc acggtcacca     1860

cagactggaa aaggcttgtc caggaatgga cggtgcagca gccagggtac acatgccagg     1920

ctgttcccga ggagcagtgt cccgtctctg acagctccca ctgccaggtc ctcctctcag     1980

cgtcgtttgc tgaatgccac aaggtcatcg ctccagccac attccatacc atctgccagc     2040

aagacagttg ccaccaggag cgagtgtgtg aggtgattgc ttcttacgcc catctctgtc     2100

ggaccagtgg ggtctgtgtt gattggagga caactgattt ctgtgctatg tcatgcccac     2160

cgtccctggt gtataaccac tgtgagcgtg gctgccctcg gcactgcgat gggaacacta     2220

gcttctgtgg ggaccatccc tcagaaggct gcttctgtcc ccaacaccaa gtttttctgg     2280

aaggcagctg tgtccccgag gaggcctgca ctcagtgtgt tggcgaggat ggagttcgac     2340

atcagttcct ggagacctgg gtcccagacc atcagccctg tcagatctgt atgtgcctca     2400

gtgggagaaa gattaactgc actgcccagc cgtgtcccac agcccgagct cccacgtgtg     2460

gcccatgtga agtggctcgc ctcaagcaga gcacaaacct gtgctgccca gagtatgagt     2520

gtgtgtgtga cctgttcaac tgcaacttgc ctccagtgcc tccgtgtgaa ggagggctcc     2580

agccaaccct gaccaaccct ggagaatgca gacccacctt tacctgtgcc tgcaggaaag     2640

aagagtgcaa aagagtgtcc ccaccctcct gcccccctca ccggacaccc actctccgga     2700

agacccagtg ctgtgatgaa tacgagtgtg cttgcagctg tgtcaactcc acgctgagct     2760

gcccacttgg ctacctggcc tcagccacta ccaatgactg tggctgcacc acgaccacct     2820

gtctccctga caaggtttgt gtccaccgag gcaccgtcta ccctgtgggc cagttctggg     2880

aggagggctg tgacacgtgc acctgtacgg acatggagga tactgtcgtg ggcctgcgtg     2940

tggtccagtg ctctcaaagg ccctgtgaag acagctgtca gccaggtttt tcttatgttc     3000

tccacgaagg cgagtgctgt ggaaggtgcc tgccctctgc ttgcaaggtg gtggctggct     3060

cactgcgggg cgattcccac tcttcctgga aaagtgttgg atctcggtgg gctgttcctg     3120

agaacccctg cctcgtcaac gagtgtgtcc gcgtggagga tgcagtgttt gtgcagcaga     3180

ggaacatctc ctgcccacag ctggctgtcc ctacctgtcc cacaggcttc caactgaact     3240

gtgagacctc agagtgctgt cctagctgcc actgtgagcc tgtggaggcc tgcctgctca     3300

atggcaccat cattgggccc gggaagagtg tgatggttga cctatgcacg acctgccgct     3360

gcatcgtgca gacagacgcc atctccagat tcaagctgga gtgcaggaag actacctgtg     3420

aggcctgccc catgggctat cgggaagaga agagccaggg tgaatgctgt gggagatgct     3480

tgcctacagc ttgcactatt cagctaagag gaggacggat catgaccctg aagcaagatg     3540

agacattcca ggatggctgt gacagtcatt tgtgcagggt caacgagaga ggagagtaca     3600

tctgggagaa gagggtcacg ggctgcccac catttgatga acacaagtgt ctggctgaag     3660

gaggcaaaat cgtgaaaatt ccaggcacct gctgtgacac atgtgaggag cctgattgca     3720

aagacatcac agccaaggtg cagtacatca aagtgggaga ttgtaagtcc caagaggaag     3780

tggacattca ttactgccag ggaaagtgtg ccagcaaagc tgtgtactcc attgacatcg     3840

aggatgtgca ggagcaatgc tcctgctgcc tgccctcgag gacggagccc atgcgcgtgc     3900

ccttgcactg caccaatggc tctgtcgtgt accacgaggt catcaacgcc atgcagtgca     3960

ggtgttctcc ccggaactgc agcaagtgac tgactgagat acagcgtacc ttcagctcac     4020

agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa     4080

atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa     4140

taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg     4200

ggaggttttt tactgcagcc aacccctgga tgtggtcctg ctcctggatg gctcctctag     4260

cttgccagag tcttcctttg ataaaatgaa gagttttgcc aaggctttca tttcaaaggc     4320

caacattggt gagtgatacc cttgaacctg caggtgaggg agtggctctt cctggttcat     4380

tgattctaaa tgtctccctt ctccttttcc tgttagggcc ccacctcaca caggtgtccg     4440

tgatacagta tggaagcatc aataccattg atgtaccatg gaatgtggtt caggagaaag     4500

cccatctaca gagtttggtg gacctcatgc agcaggaggg tggccccagc cagattggta     4560

atgcttggag ccacgagcta gatgtagaac ttgtgttctg atcctcactc ttgtgttctg     4620

attgagtgat cttgaccagt aactttactc cttggtctga gtttctcttt tgattggcga     4680

gaagctagat ggtcctttgt gtcattttcc agtcccacca agtattgttc tgagtcataa     4740

ctgctcatct tttgaatgta cctgagtcag cccttaagcc cattgctcag agggtctaga     4800

atactccatc c                                                          4811


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target site

<400>  8
tgcagactgc agccaacccc tgg                                               23


<210>  9
<211>  4750
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct

<400>  9
gcttgaggtc tgagcttacc acctctgacg cacaagtggg cttcttgatg tcagctctgg       60

ttacgggtgg tggcagggag ggacacgtgg cagtgggcag atcactatag attttaacat      120

gtagctacat acatcttaac gtgtagctat gcacacagga ctgctcctgg cagaagtgcg      180

tacttcatca ctcttttcta tactctgggc tttcccactg ttctgtcttg tttttcccat      240

tagcctcagg ctttcaacat cagtgtgtct gttttacaga caccctgtgg ccaatctcag      300

gtagatgtgg ctttcagggt gaggctgagc gaattcataa caggaggcct aaagagcatc      360

cgggcctcct ccctggctgc ctggctcact ttggacaacc ccttcccttc tttgcctcag      420

tttccccctt ttagggacag ccactaggct tccctgtctc ctgctgggcc ccatgctggg      480

cctatgaagt ccacactcca cgctacaggt cctcaacttc cttgggcttc ctggagggtt      540

gggaggcacc cagagtattc tgtgttcctt cattgcctcc atggcccaga tgggcccctc      600

aaacccaagg tgcccaactt gtcatctctg ccatgactgc tcctagcgtt acataactta      660

cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga      720

cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt      780

tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta      840

ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg      900

actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt      960

tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc     1020

accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat     1080

gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct     1140

atataagcag agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatgc     1200

caccatgatt cctgccagat ttgccggggt gctgcttgct ctggccctca ttttgccagg     1260

gaccctttgt gcagaaggaa ctcgcggcag gtcatccacg gcccgatgca gccttttcgg     1320

aagtgacttc gtcaacacct ttgatgggag catgtacagc tttgcgggat actgcagtta     1380

cctcctggca gggggctgcc agaaacgctc cttctcgatt attggggact tccagaatgg     1440

caagagagtg agcctctccg tgtatcttgg ggaatttttt gacatccatt tgtttgtcaa     1500

tggtaccgtg acacaggggg accaaagagt ctccatgccc tatgcctcca aagggctgta     1560

tctagaaact gaggctgggt actacaagct gtccggtgag gcctatggct ttgtggccag     1620

gatcgatggc agcggcaact ttcaagtcct gctgtcagac agatacttca acaagacctg     1680

cgggctgtgt ggcaacttta acatctttgc tgaagatgac tttatgaccc aagaagggac     1740

cttgacctcg gacccttatg actttgccaa ctcatgggct ctgagcagtg gagaacagtg     1800

gtgtgaacgg gcatctcctc ccagcagctc atgcaacatc tcctctgggg aaatgcagaa     1860

gggcctgtgg gagcagtgcc agcttctgaa gagcacctcg gtgtttgccc gctgccaccc     1920

tctggtggac cccgagcctt ttgtggccct gtgtgagaag actttgtgtg agtgtgctgg     1980

ggggctggag tgcgcctgcc ctgccctcct ggagtacgcc cggacctgtg cccaggaggg     2040

aatggtgctg tacggctgga ccgaccacag cgcgtgcagc ccagtgtgcc ctgctggtat     2100

ggagtatagg cagtgtgtgt ccccttgcgc caggacctgc cagagcctgc acatcaatga     2160

aatgtgtcag gagcgatgcg tggatggctg cagctgccct gagggacagc tcctggatga     2220

aggcctctgc gtggagagca ccgagtgtcc ctgcgtgcat tccggaaagc gctaccctcc     2280

cggcacctcc ctctctcgag actgcaacac ctgcatttgc cgaaacagcc agtggatctg     2340

cagcaatgaa gaatgtccag gggagtgcct tgtcacaggt caatcacact tcaagagctt     2400

tgacaacaga tacttcacct tcagtgggat ctgccagtac ctgctggccc gggattgcca     2460

ggaccactcc ttctccattg tcattgagac tgtccagtgt gctgatgacc gcgacgctgt     2520

gtgcacccgc tccgtcaccg tccggctgcc tggcctgcac aacagccttg tgaaactgaa     2580

gcatggggca ggagttgcca tggatggcca ggacgtccag ctccccctcc tgaaaggtga     2640

cctccgcatc cagcatacag tgacggcctc cgtgcgcctc agctacgggg aggacctgca     2700

gatggactgg gatggccgcg ggaggctgct ggtgaagctg tcccccgtct atgccgggaa     2760

gacctgcggc ctgtgtggga attacaatgg caaccagggc gacgacttcc ttaccccctc     2820

tgggctggcg gagccccggg tggaggactt cgggaacgcc tggaagctgc acggggactg     2880

ccaggacctg cagaagcagc acagcgatcc ctgcgccctc aacccgcgca tgaccaggtt     2940

ctccgaggag gcgtgcgcgg tcctgacgtc ccccacattc gaggcctgcc atcgtgccgt     3000

cagcccgctg ccctacctgc ggaactgccg ctacgacgtg tgctcctgct cggacggccg     3060

cgagtgcctg tgcggcgccc tggccagcta tgccgcggcc tgcgcgggga gaggcgtgcg     3120

cgtcgcgtgg cgcgagccag gccgctgtga gctgaactgc ccgaaaggcc aggtgtacct     3180

gcagtgcggg accccctgca acctgacctg ccgctctctc tcttacccgg atgaggaatg     3240

caatgaggcc tgcctggagg gctgcttctg ccccccaggg ctctacatgg atgagagggg     3300

ggactgcgtg cccaaggccc agtgcccctg ttactatgac ggtgagatct tccagccaga     3360

agacatcttc tcagaccatc acaccatgtg ctactgtgag gatggcttca tgcactgtac     3420

catgagtgga gtccccggaa gcttgctgcc tgacgctgtc ctcagcagtc ccctgtctca     3480

tcgcagcaaa aggagcctat cctgtcggcc ccccatggtc aagctggtgt gtcccgctga     3540

caacctgcgg gctgaagggc tcgagtgtac caaaacgtgc cagaactatg acctggagtg     3600

catgagcatg ggctgtgtct ctggctgcct ctgccccccg ggcatggtcc ggcatgagaa     3660

cagatgtgtg gccctggaaa ggtgtccctg cttccatcag ggcaaggagt atgcccctgg     3720

agaaacagtg aagattggct gcaacacttg tgtctgtcgg gaccggaagt ggaactgcac     3780

agaccatgtg tgtgatgcca cgtgctccac gatcggcatg gcccactacc tcacgttcga     3840

cgggctcaaa tacctgttcc ccggggagtg ccagtacgtt ctggtgcagg tgagaggtgg     3900

ggagatgggg agagggtgct gtttctttct aggaggggtg ggaggtgtgg cctcaggttg     3960

ggttctgtgg atctgtctgc agaaacaact ctggggtctg gtttctactg gagtacttcc     4020

cagtccttca cagaagtgcc tgaagcggta ggggatttga agctcaaagt ggttgtccat     4080

tttccctctg ctcacctggg gacttataaa acgagacaga agcttgtttg ttgttgagga     4140

ttggtgtggg agaaaggcta ctgctagtcc acattagcac agatgtggaa ttagaaaaag     4200

tcatctgttc cttctggtag acacagcctc agtcagggtg catagcttag ggagtgggtt     4260

gggctgggaa gtcagtcccg ctcagcctcc cttccagcac cctgggcagt gcacagtctg     4320

caggtgttgt gcagtggccc tggacagggg gatggttgaa atgacccctg gagtttgctt     4380

cccacggtat ggctttgtgg aattctccgc cattttaatg tctaacttgg tacaattcag     4440

aatgggagga gtgggaggat gggacacagg aaagtcatcc tgcccagcag atgagagcga     4500

tccaggaatc ctcacggtga gtgtgggcag cagcccctct gcctcccact ccccactgcg     4560

tggattcttg taagtttctc tttctggttg acatcaactg tgtaagcaag gaagtatgag     4620

tgcttttctc accagagctg aggcactgta ctctgtgaag ctttgaacaa atatggtccc     4680

tctgtctcca ttcccaggag gaggaggggc gggagcttgg tgtggtctga atggaagacc     4740

acaaacccat                                                            4750


<210>  10
<211>  4750
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct

<400>  10
agagatgctt aaaatcattg ccatgttgaa aacctatcta ggagaccact atatttaatg       60

actaattgtc aataaaatac ctgctcattg gtttcatagt actttaattt cataatcatg      120

attttgctgc tacctctgtt accgtctctt ggtcatggat gcctggagag tggtggtggt      180

gagatggtca cagacatgtc ctggcgtggg gctggccctg caggggtgca gtggcaggtg      240

gggtcctgga ggggtggcag tgcctgcact cgtgggcact gaagacagat gggcaggtgt      300

agagtggagg gaggatctgg ctgtcgagcc tgcccttcat cctcctggat ttcttgcttt      360

gtcttcctcc agcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga      420

cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt      480

ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt      540

gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca      600

ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt      660

catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt      720

tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca      780

ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg      840

cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc      900

cactgcttac tggcttatcg aaatgccacc atgattcctg ccagatttgc cggggtgctg      960

cttgctctgg ccctcatttt gccagggacc ctttgtgcag aaggaactcg cggcaggtca     1020

tccacggccc gatgcagcct tttcggaagt gacttcgtca acacctttga tgggagcatg     1080

tacagctttg cgggatactg cagttacctc ctggcagggg gctgccagaa acgctccttc     1140

tcgattattg gggacttcca gaatggcaag agagtgagcc tctccgtgta tcttggggaa     1200

ttttttgaca tccatttgtt tgtcaatggt accgtgacac agggggacca aagagtctcc     1260

atgccctatg cctccaaagg gctgtatcta gaaactgagg ctgggtacta caagctgtcc     1320

ggtgaggcct atggctttgt ggccaggatc gatggcagcg gcaactttca agtcctgctg     1380

tcagacagat acttcaacaa gacctgcggg ctgtgtggca actttaacat ctttgctgaa     1440

gatgacttta tgacccaaga agggaccttg acctcggacc cttatgactt tgccaactca     1500

tgggctctga gcagtggaga acagtggtgt gaacgggcat ctcctcccag cagctcatgc     1560

aacatctcct ctggggaaat gcagaagggc ctgtgggagc agtgccagct tctgaagagc     1620

acctcggtgt ttgcccgctg ccaccctctg gtggaccccg agccttttgt ggccctgtgt     1680

gagaagactt tgtgtgagtg tgctgggggg ctggagtgcg cctgccctgc cctcctggag     1740

tacgcccgga cctgtgccca ggagggaatg gtgctgtacg gctggaccga ccacagcgcg     1800

tgcagcccag tgtgccctgc tggtatggag tataggcagt gtgtgtcccc ttgcgccagg     1860

acctgccaga gcctgcacat caatgaaatg tgtcaggagc gatgcgtgga tggctgcagc     1920

tgccctgagg gacagctcct ggatgaaggc ctctgcgtgg agagcaccga gtgtccctgc     1980

gtgcattccg gaaagcgcta ccctcccggc acctccctct ctcgagactg caacacctgc     2040

atttgccgaa acagccagtg gatctgcagc aatgaagaat gtccagggga gtgccttgtc     2100

acaggtcaat cacacttcaa gagctttgac aacagatact tcaccttcag tgggatctgc     2160

cagtacctgc tggcccggga ttgccaggac cactccttct ccattgtcat tgagactgtc     2220

cagtgtgctg atgaccgcga cgctgtgtgc acccgctccg tcaccgtccg gctgcctggc     2280

ctgcacaaca gccttgtgaa actgaagcat ggggcaggag ttgccatgga tggccaggac     2340

gtccagctcc ccctcctgaa aggtgacctc cgcatccagc atacagtgac ggcctccgtg     2400

cgcctcagct acggggagga cctgcagatg gactgggatg gccgcgggag gctgctggtg     2460

aagctgtccc ccgtctatgc cgggaagacc tgcggcctgt gtgggaatta caatggcaac     2520

cagggcgacg acttccttac cccctctggg ctggcggagc cccgggtgga ggacttcggg     2580

aacgcctgga agctgcacgg ggactgccag gacctgcaga agcagcacag cgatccctgc     2640

gccctcaacc cgcgcatgac caggttctcc gaggaggcgt gcgcggtcct gacgtccccc     2700

acattcgagg cctgccatcg tgccgtcagc ccgctgccct acctgcggaa ctgccgctac     2760

gacgtgtgct cctgctcgga cggccgcgag tgcctgtgcg gcgccctggc cagctatgcc     2820

gcggcctgcg cggggagagg cgtgcgcgtc gcgtggcgcg agccaggccg ctgtgagctg     2880

aactgcccga aaggccaggt gtacctgcag tgcgggaccc cctgcaacct gacctgccgc     2940

tctctctctt acccggatga ggaatgcaat gaggcctgcc tggagggctg cttctgcccc     3000

ccagggctct acatggatga gaggggggac tgcgtgccca aggcccagtg cccctgttac     3060

tatgacggtg agatcttcca gccagaagac atcttctcag accatcacac catgtgctac     3120

tgtgaggatg gcttcatgca ctgtaccatg agtggagtcc ccggaagctt gctgcctgac     3180

gctgtcctca gcagtcccct gtctcatcgc agcaaaagga gcctatcctg tcggcccccc     3240

atggtcaagc tggtgtgtcc cgctgacaac ctgcgggctg aagggctcga gtgtaccaaa     3300

acgtgccaga actatgacct ggagtgcatg agcatgggct gtgtctctgg ctgcctctgc     3360

cccccgggca tggtccggca tgagaacaga tgtgtggccc tggaaaggtg tccctgcttc     3420

catcagggca aggagtatgc ccctggagaa acagtgaaga ttggctgcaa cacttgtgtc     3480

tgtcgggacc ggaagtggaa ctgcacagac catgtgtgtg atgccacgtg ctccacgatc     3540

ggcatggccc actacctcac cttcgacggg ctcaaatacc tgttccccgg ggagtgccag     3600

tacgttctgg tgcaggatta ctgcggcagt aaccctggga cctttcggat cctagtgggg     3660

aataagggat gcagccaccc ctcagtgaaa tgcaagaaac gggtcaccat cctggtggag     3720

ggaggagaga ttgagctgtt tgacggggag gtgaatgtga agaggcccat gaaggatgag     3780

actcactttg aggtggtgga gtctggccgg tacatcattc tgctgctggg caaagccctc     3840

tccgttgtct gggaccgcca cctgagcatc tccgtggtcc tgaagcagac ataccaggtc     3900

agtggctttc ttgcttcatc ttgttgggga cttggccttt ggagtgtttt ctgctccctg     3960

atcgtaggtc tctaaggact tgctttatga atccaggtgc tcctgtgttg ggtgcatata     4020

tatttaggat agttagctct tcttgttgaa ttgatccctt taccattatg taatggcctt     4080

cttttgatct ttgttggttc aaagactgtt ttatcagata ctaggattgc aacccctgct     4140

tttttttttt tgccttccat ttgcttggta gaccttcctc cctcccttta ttttgagcct     4200

atgtatgtct ctgcacgtga gatgggtctc ctgaatacag cacactgatg ggtcttgact     4260

ctttatccaa ttggccagtc tgtgcctttt aattggggca tttagcccat ttacatttaa     4320

ggttaatatt gtcatgtgtg aatttgatcc tgtcattatg atgttcgctg gttattttgc     4380

ccattaattg ataccgtttc ttcgtagcat cgatggtctt tacaatttgg catgtttttg     4440

cagtggctgg tactggttgt ttctttccat gtttagtgct tccttcagga gctcttgtaa     4500

ggcaggtctg gtggtgacaa aatctctcag catttgcttg tctgtaaagg attttatttc     4560

tctttcactt atgaagctta gtttgggtgg atatgaaatt ctaagttgaa aattcttttc     4620

tttaagaatg ttgaatattg gtccccccct ctcttctggc ttgtagggtt tctgccaaga     4680

gatctgctat tagtctgatg ggcttccctt tgtgagtaac tcgacatttc tctctggctg     4740

cccttaacac                                                            4750


<210>  11
<211>  4982
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct

<400>  11
tagccacacg cagtttgtga cgatcctatg gtacagcaca gctctagagt tactgaaggt       60

gttattcaga agagcagaaa gagccccgga gataagattt catttgtcct gaggcttggg      120

gaggtgaggt agggtgaagg aatccccgct cccagttttg cagagggatc aatcaaggca      180

caagcaggag agatgctcct tgagtgatgg ggtgacccct gggagtgcag gcaggaggag      240

ttggcttcta gggcaggagg aggagttggc tcctcccttt tagttaaaaa tgaggcttcc      300

tcgtgggaaa ggggagcgtt ttggttccta atgagagctt tcttttgcag cgttacataa      360

cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata      420

atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag      480

tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc      540

cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta      600

tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg      660

cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt      720

ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca      780

aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag      840

gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa      900

atgccaccat gattcctgcc agatttgccg gggtgctgct tgctctggcc ctcattttgc      960

cagggaccct ttgtgcagaa ggaactcgcg gcaggtcatc cacggcccga tgcagccttt     1020

tcggaagtga cttcgtcaac acctttgatg ggagcatgta cagctttgcg ggatactgca     1080

gttacctcct ggcagggggc tgccagaaac gctccttctc gattattggg gacttccaga     1140

atggcaagag agtgagcctc tccgtgtatc ttggggaatt ttttgacatc catttgtttg     1200

tcaatggtac cgtgacacag ggggaccaaa gagtctccat gccctatgcc tccaaagggc     1260

tgtatctaga aactgaggct gggtactaca agctgtccgg tgaggcctat ggctttgtgg     1320

ccaggatcga tggcagcggc aactttcaag tcctgctgtc agacagatac ttcaacaaga     1380

cctgcgggct gtgtggcaac tttaacatct ttgctgaaga tgactttatg acccaagaag     1440

ggaccttgac ctcggaccct tatgactttg ccaactcatg ggctctgagc agtggagaac     1500

agtggtgtga acgggcatct cctcccagca gctcatgcaa catctcctct ggggaaatgc     1560

agaagggcct gtgggagcag tgccagcttc tgaagagcac ctcggtgttt gcccgctgcc     1620

accctctggt ggaccccgag ccttttgtgg ccctgtgtga gaagactttg tgtgagtgtg     1680

ctggggggct ggagtgcgcc tgccctgccc tcctggagta cgcccggacc tgtgcccagg     1740

agggaatggt gctgtacggc tggaccgacc acagcgcgtg cagcccagtg tgccctgctg     1800

gtatggagta taggcagtgt gtgtcccctt gcgccaggac ctgccagagc ctgcacatca     1860

atgaaatgtg tcaggagcga tgcgtggatg gctgcagctg ccctgaggga cagctcctgg     1920

atgaaggcct ctgcgtggag agcaccgagt gtccctgcgt gcattccgga aagcgctacc     1980

ctcccggcac ctccctctct cgagactgca acacctgcat ttgccgaaac agccagtgga     2040

tctgcagcaa tgaagaatgt ccaggggagt gccttgtcac aggtcaatca cacttcaaga     2100

gctttgacaa cagatacttc accttcagtg ggatctgcca gtacctgctg gcccgggatt     2160

gccaggacca ctccttctcc attgtcattg agactgtcca gtgtgctgat gaccgcgacg     2220

ctgtgtgcac ccgctccgtc accgtccggc tgcctggcct gcacaacagc cttgtgaaac     2280

tgaagcatgg ggcaggagtt gccatggatg gccaggacgt ccagctcccc ctcctgaaag     2340

gtgacctccg catccagcat acagtgacgg cctccgtgcg cctcagctac ggggaggacc     2400

tgcagatgga ctgggatggc cgcgggaggc tgctggtgaa gctgtccccc gtctatgccg     2460

ggaagacctg cggcctgtgt gggaattaca atggcaacca gggcgacgac ttccttaccc     2520

cctctgggct ggcggagccc cgggtggagg acttcgggaa cgcctggaag ctgcacgggg     2580

actgccagga cctgcagaag cagcacagcg atccctgcgc cctcaacccg cgcatgacca     2640

ggttctccga ggaggcgtgc gcggtcctga cgtcccccac attcgaggcc tgccatcgtg     2700

ccgtcagccc gctgccctac ctgcggaact gccgctacga cgtgtgctcc tgctcggacg     2760

gccgcgagtg cctgtgcggc gccctggcca gctatgccgc ggcctgcgcg gggagaggcg     2820

tgcgcgtcgc gtggcgcgag ccaggccgct gtgagctgaa ctgcccgaaa ggccaggtgt     2880

acctgcagtg cgggaccccc tgcaacctga cctgccgctc tctctcttac ccggatgagg     2940

aatgcaatga ggcctgcctg gagggctgct tctgcccccc agggctctac atggatgaga     3000

ggggggactg cgtgcccaag gcccagtgcc cctgttacta tgacggtgag atcttccagc     3060

cagaagacat cttctcagac catcacacca tgtgctactg tgaggatggc ttcatgcact     3120

gtaccatgag tggagtcccc ggaagcttgc tgcctgacgc tgtcctcagc agtcccctgt     3180

ctcatcgcag caaaaggagc ctatcctgtc ggccccccat ggtcaagctg gtgtgtcccg     3240

ctgacaacct gcgggctgaa gggctcgagt gtaccaaaac gtgccagaac tatgacctgg     3300

agtgcatgag catgggctgt gtctctggct gcctctgccc cccgggcatg gtccggcatg     3360

agaacagatg tgtggccctg gaaaggtgtc cctgcttcca tcagggcaag gagtatgccc     3420

ctggagaaac agtgaagatt ggctgcaaca cttgtgtctg tcgggaccgg aagtggaact     3480

gcacagacca tgtgtgtgat gccacgtgct ccacgatcgg catggcccac tacctcacct     3540

tcgacgggct caaatacctg ttccccgggg agtgccagta cgttctggtg caggattact     3600

gcggcagtaa ccctgggacc tttcggatcc tagtggggaa taagggatgc agccacccct     3660

cagtgaaatg caagaaacgg gtcaccatcc tggtggaggg aggagagatt gagctgtttg     3720

acggggaggt gaatgtgaag aggcccatga aggatgagac tcactttgag gtggtggagt     3780

ctggccggta catcattctg ctgctgggca aagccctctc cgtggtctgg gaccgccacc     3840

tgagcatctc cgtggtcctg aagcagacat accaggagaa agtgtgtggc ctgtgtggga     3900

attttgatgg catccagaac aatgacctca ccagcagcaa cctccaagtg gaggaagacc     3960

ctgtggactt tgggaactcc tggaaagtga gctcgcagtg tgctgacacc agaaaagtgc     4020

ctctggactc atcccctgcc acctgccata acaacatcat gaagcagacg atggtggatt     4080

cctcctgtag aatccttacc agtgacgtct tccaggactg caacaagctg gtggaccccg     4140

agccatatct ggatgtctgc atttacgaca cctgctcctg tgagtccatt ggggactgcg     4200

cctgcttctg cgacaccatt gctgcctatg cccacgtgtg tgcccagcat ggcaaggtgg     4260

tgacctggag gacggccaca ttgtgccccc agagctgcga ggagaggaat ctccgggaga     4320

acgggtatga gtgtgagtgg cgctataaca gctgtgcacc tgcctgtcaa gtcacgtgtc     4380

agcaccctga gccactggcc tgccctgtgc agtgtgtgga gggctgccat gcccactgcc     4440

ctccagggaa aatcctggat gagcttttgc agacctgcgt tgaccctgaa gactgtccag     4500

tgtgtgaggt ggctggtcgg cgttttgcct caggaaagaa agtcaccttg aatcccagtg     4560

accctgagca ctgccagatt tgtaaaacag attcctgggt tgtttgaagt gatgaatctt     4620

attgcttctc caggggccag cttagagact aggttttggg taaaaagtcc tagccacatg     4680

agttgagaga ctgagtttat tttatttgtt tatttattta attcattatt taatgtagtt     4740

tattgttttg tttgttccta gggtttgctt ctttttcttt agggatggac tagactgctt     4800

cctccacata tattcctcca cagtgttttt gctcttaata ggtttttaag tcctaacagg     4860

tttttgcttt tctcactgaa ggtgggggta tggtcactta ttgtccttgt aggtcatgac     4920

tagtctgagc acaggtgtcc atatactgga ggtggggact caggagagga aagtgagatg     4980

ga                                                                    4982


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target site

<400>  12
caggtatttg agcccgtcga                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target site

<400>  13
gctgggcaaa gccctctccg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target site

<400>  14
tctttcctga ggcaaaacgc                                                   20


<210>  15
<211>  4700
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct

<400>  15
gtgggcagag ccattctcac taacttgcct tgtgtgagct aagaccattt ctttgccttg       60

tgatttattc catccttatg gcagttgggc tgactccttg acgtgacaat atcaacagct      120

gcgtgttggg gatcacttgc agcatggatg ttagtaagcc agtagtattc agtactgtca      180

cttggaaatg actgactgct caaggacata ggggattttt agtagaactt ctggggaggc      240

tgcttagctc tgatggtgct tccagaccca tcatttgtca gtggttgtgc tgcttgacag      300

taacccccat agggagaggt tggctcaggc ttgactatga gtgagggatg ttagtggggc      360

ctccctgctc caccgaatct gtgtctcagc tcttgggcat gtattcacct gccattgtag      420

atgtggtcgt ggtttgtctg gaaagcttat ctccaacttt acttaggtcc aagattagct      480

ttcaaatcat ttatggtggt ggtgccttca atgtgaggat ttttaccaaa tcatcttatg      540

taggtgcaaa atgatcagta ttcaccaata gtgttttaat aggaatcttg agaatttgcc      600

aattatgtga atgactcagg gtcaaatgag ggagcattga gactcaacca tttttttttt      660

tttttttttt ttggctctgg aaatatttct cctgtgcgat ctcttaaggg attaagtcaa      720

catggtcact tgtaccatta ccttagtcac ttggcagctg aaaggacacg tgtctttagg      780

tgaattttct ttacaatgag atcatgagct catagcttgc ttagcttttc ttaatttcca      840

cgagtcataa cacccctgga acttaacttt gttttctggg ccaaggcatc cctgtagaat      900

ggattattca cactgtacat ttaaattttt tagaagtgtg gttctataat ttgccacatt      960

ttatgtaaca ggaaaatatt taatggccaa gtgttactta cctaaacctc tctacctctc     1020

agagccccag tttcctaatc tgtaaaaaaa ggaggaaatt gttctatatg acctcaaagg     1080

gcctgttccg ttctctactg tatttatctg tgtgcaactt ggtcacacct gcctgtctgc     1140

atgtagtagg catgggggtt tggataacgt cgcatccatc ctctgcttct ctctgtccag     1200

gcgtgtgcac aggcagcagt actcggcaca tcgtgacctt tgatgggcag aatttcaagc     1260

tgactggcag ctgttcttat gtcctatttc aaaacaagga gcaggacctg gaggtgattc     1320

tccataatgg tgcctgcagc cctggagcaa ggcagggctg catgaaatcc atcgaggtga     1380

agcacagtgc cctctccgtc gagctgcaca gtgacatgga ggtgacggtg aatgggagac     1440

tggtctctgt tccttacgtg ggtgggaaca tggaagtcaa cgtttatggt gccatcatgc     1500

atgaggtcag attcaatcac cttggtcaca tcttcacatt cactccacaa aacaatgagt     1560

tccaactgca gctcagcccc aagacttttg cttcaaagac gtatggtctg tgtgggatct     1620

gtgatgagaa cggagccaat gacttcatgc tgagggatgg cacagtcacc acagactgga     1680

aaacacttgt tcaggaatgg actgtgcagc ggccagggca gacgtgccag cccatcctgg     1740

aggagcagtg tcttgtcccc gacagctccc actgccaggt cctcctctta ccactgtttg     1800

ctgaatgcca caaggtcctg gctccagcca cattctatgc catctgccag caggacagtt     1860

gccaccagga gcaagtgtgt gaggtgatcg cctcttatgc ccacctctgt cggaccaacg     1920

gggtctgcgt tgactggagg acacctgatt tctgtgctat gtcatgccca ccatctctgg     1980

tctacaacca ctgtgagcat ggctgtcccc ggcactgtga tggcaacgtg agctcctgtg     2040

gggaccatcc ctccgaaggc tgtttctgcc ctccagataa agtcatgttg gaaggcagct     2100

gtgtccctga agaggcctgc actcagtgca ttggtgagga tggagtccag caccagttcc     2160

tggaagcctg ggtcccggac caccagccct gtcagatctg cacatgcctc agcgggcgga     2220

aggtcaactg cacaacgcag ccctgcccca cggccaaagc tcccacgtgt ggcctgtgtg     2280

aagtagcccg cctccgccag aatgcagacc agtgctgccc cgagtatgag tgtgtgtgtg     2340

acccagtgag ctgtgacctg cccccagtgc ctcactgtga acgtggcctc cagcccacac     2400

tgaccaaccc tggcgagtgc agacccaact tcacctgcgc ctgcaggaag gaggagtgca     2460

aaagagtgtc cccaccctcc tgccccccgc accgtttgcc cacccttcgg aagacccagt     2520

gctgtgatga gtatgagtgt gcctgcaact gtgtcaactc cacagtgagc tgtccccttg     2580

ggtacttggc ctcaactgcc accaatgact gtggctgtac cacaaccacc tgccttcccg     2640

acaaggtgtg tgtccaccga agcaccatct accctgtggg ccagttctgg gaggagggct     2700

gcgatgtgtg cacctgcacc gacatggagg atgccgtgat gggcctccgc gtggcccagt     2760

gctcccagaa gccctgtgag gacagctgtc ggtcgggctt cacttacgtt ctgcatgaag     2820

gcgagtgctg tggaaggtgc ctgccatctg cctgtgaggt ggtgactggc tcaccgcggg     2880

gggactccca gtcttcctgg aagagtgtcg gctcccagtg ggcctccccg gagaacccct     2940

gcctcatcaa tgagtgtgtc cgagtgaagg aggaggtctt tatacaacaa aggaacgtct     3000

cctgccccca gctggaggtc cctgtctgcc cctcgggctt tcagctgagc tgtaagacct     3060

cagcgtgctg cccaagctgt cgctgtgagc gcatggaggc ctgcatgctc aatggcactg     3120

tcattgggcc cgggaagact gtgatgatcg atgtgtgcac gacctgccgc tgcatggtgc     3180

aggtgggggt catctctgga ttcaagctgg agtgcaggaa gaccacctgc aacccctgcc     3240

ccctgggtta caaggaagaa aataacacag gtgaatgttg tgggagatgt ttgcctacgg     3300

cttgcaccat tcagctaaga ggaggacaga tcatgacact gaagcgtgat gagacgctcc     3360

aggatggctg tgatactcac ttctgcaagg tcaatgagag aggagagtac ttctgggaga     3420

agagggtcac aggctgccca ccctttgatg aacacaagtg tctggctgag ggaggtaaaa     3480

ttatgaaaat tccaggcacc tgctgtgaca catgtgagga gcctgagtgc aacgacatca     3540

ctgccaggct gcagtatgtc aaggtgggaa gctgtaagtc tgaagtagag gtggatatcc     3600

actactgcca gggcaaatgt gccagcaaag ccatgtactc cattgacatc aacgatgtgc     3660

aggaccagtg ctcctgctgc tctccgacac ggacggagcc catgcaggtg gccctgcact     3720

gcaccaatgg ctctgttgtg taccatgagg ttctcaatgc catggagtgc aaatgctccc     3780

ccaggaagtg cagcaagtga ctgactgaaa cttgtttatt gcagcttata atggttacaa     3840

ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg     3900

tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcgtgagaa gtactttctg     3960

tggatccgtg gtaaggcaat agaatgtcag gaaaaccacc tggacctggt ggcagttgct     4020

tttagttgat gctcttgtta ggagctctgc cttctgctta agtggaggag aggagtacca     4080

ctttcttaga ggggtttatt gccatcccct tgtcttggcg tgatttcatg ttgttccggg     4140

ctcagatttg caagatggaa tcacttttag atagcataaa attgtgaatt tagtgccagt     4200

ttctggcact ggtggagaat tgggattggc atcaggattg tttactcgga aggtattatg     4260

agtccaatgc ctaaaccctg taagctttcc aaagggaaac atttatggcc taaattaggt     4320

cttttgaaaa tatttaaggc ctacataaaa cgtcaggctc caaaatttga aaagaaaact     4380

gcaaaactga tatatatata tataaatgat tgattaaatg cttacaaaag gttacactat     4440

gccaacttct ttacttgttc gtgtagaaat cataaatatt tcattgtaat gtgaaaacaa     4500

cttgtaagct agattttctc acttcgcaag aattcctgaa tttgaacaat aattgcagaa     4560

aaatcttagt catatatcaa gtgagtaact catagccaaa aattaaaaaa tcaaaatgat     4620

aaaaaatcct tccaaaaatt ttacagcaaa attatattca ttgtggaatg tgagtacatt     4680

ttaatgtgtt tgatatgata                                                 4700


<210>  16
<211>  4700
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct

<400>  16
cttccccatg ggcctagact aactctcaac atgggtataa agggctttag aaatacgata       60

acacggagac tcatatcaaa gtaccatagt ttaagttgat tttaggttag aaacttaaaa      120

aatatgcttt tggccaggtg cagtggctca cgtctgtaat cccagcactt tgggaggccg      180

aggcgggcgg atcacgaggt caggagatcg agaccatctt ggctaacaca gtgaaaccct      240

gtctctacta aaaatacaaa aaattagccg ggcgtggtgg cgggcacctg tagtaccagc      300

tacttgggag gctgaggcag gagaatggtg tgaacccggg aggcagagct tgcagtgagc      360

cgagatcgcg ccactgtact ccagcctggg caacagagtg agactccatc tcaaaaaaaa      420

aaaaaaaata cacacacaca cacacacacg ctttttttgt ggcgggggcc tgggtttgta      480

tattttcccg ttactagatg taagtcaaaa cctgcataaa gctactgtcc ttcgggggaa      540

taagtcaatg caagtttgcc cttaaagggc aataactcta tgcaagtttt gacttatagc      600

taataacatt agctgtacag agagatggca gctctcctgg taggaatctt caagtagatc      660

tctttcaggt ttccaggatc ttgcttcatc tccccacctt ccccatccct ggcgtgatct      720

acatgtgaac caagataatg acagcgtaag ctgtagttat tgccatatta tcgctgttgt      780

tggcatcata attattaata actgcagagc atgtctgaag aaccacagga tgaccacctc      840

agcctcatgt ccctatgtct ccactgttaa ccttgttcag attcttttca gagttgagtt      900

gacttcaaaa actagaccag gttgcttaag cagacattgt gaatggttca gaatttctgg      960

gtgaaagatg ggaactaagg tcttatttgt gtctgttgca ggatttgtta ggatttgcat     1020

ggatgaggat ggtaatgaga agaggcccgg ggacgtctgg accttgccag accagtgcca     1080

caccgtgact tgccagccag atggccagac cttgctgaag agtcatcggg tcaactgtga     1140

ccgggggctg aggccttcgt gccctaacag ccagtcccct gttaaagtgg aagagacctg     1200

tggctgccgc tggacctgcc cctgcgtgtg cacaggcagc tccactcggc acatcgtgac     1260

ctttgatggg cagaatttca agctgactgg cagctgttct tatgtcctat ttcaaaacaa     1320

ggagcaggac ctggaggtga ttctccataa tggtgcctgc agccctggag caaggcaggg     1380

ctgcatgaaa tccatcgagg tgaagcacag tgccctctcc gtcgagctgc acagtgacat     1440

ggaggtgacg gtgaatggga gactggtctc tgttccttac gtgggtggga acatggaagt     1500

caacgtttat ggtgccatca tgcatgaggt cagattcaat caccttggtc acatcttcac     1560

attcactcca caaaacaatg agttccaact gcagctcagc cccaagactt ttgcttcaaa     1620

gacgtatggt ctgtgtggga tctgtgatga gaacggagcc aatgacttca tgctgaggga     1680

tggcacagtc accacagact ggaaaacact tgttcaggaa tggactgtgc agcggccagg     1740

gcagacgtgc cagcccatcc tggaggagca gtgtcttgtc cccgacagct cccactgcca     1800

ggtcctcctc ttaccactgt ttgctgaatg ccacaaggtc ctggctccag ccacattcta     1860

tgccatctgc cagcaggaca gttgccacca ggagcaagtg tgtgaggtga tcgcctctta     1920

tgcccacctc tgtcggacca acggggtctg cgttgactgg aggacacctg atttctgtgc     1980

tatgtcatgc ccaccatctc tggtctacaa ccactgtgag catggctgtc cccggcactg     2040

tgatggcaac gtgagctcct gtggggacca tccctccgaa ggctgtttct gccctccaga     2100

taaagtcatg ttggaaggca gctgtgtccc tgaagaggcc tgcactcagt gcattggtga     2160

ggatggagtc cagcaccagt tcctggaagc ctgggtcccg gaccaccagc cctgtcagat     2220

ctgcacatgc ctcagcgggc ggaaggtcaa ctgcacaacg cagccctgcc ccacggccaa     2280

agctcccacg tgtggcctgt gtgaagtagc ccgcctccgc cagaatgcag accagtgctg     2340

ccccgagtat gagtgtgtgt gtgacccagt gagctgtgac ctgcccccag tgcctcactg     2400

tgaacgtggc ctccagccca cactgaccaa ccctggcgag tgcagaccca acttcacctg     2460

cgcctgcagg aaggaggagt gcaaaagagt gtccccaccc tcctgccccc cgcaccgttt     2520

gcccaccctt cggaagaccc agtgctgtga tgagtatgag tgtgcctgca actgtgtcaa     2580

ctccacagtg agctgtcccc ttgggtactt ggcctcaact gccaccaatg actgtggctg     2640

taccacaacc acctgccttc ccgacaaggt gtgtgtccac cgaagcacca tctaccctgt     2700

gggccagttc tgggaggagg gctgcgatgt gtgcacctgc accgacatgg aggatgccgt     2760

gatgggcctc cgcgtggccc agtgctccca gaagccctgt gaggacagct gtcggtcggg     2820

cttcacttac gttctgcatg aaggcgagtg ctgtggaagg tgcctgccat ctgcctgtga     2880

ggtggtgact ggctcaccgc ggggggactc ccagtcttcc tggaagagtg tcggctccca     2940

gtgggcctcc ccggagaacc cctgcctcat caatgagtgt gtccgagtga aggaggaggt     3000

ctttatacaa caaaggaacg tctcctgccc ccagctggag gtccctgtct gcccctcggg     3060

ctttcagctg agctgtaaga cctcagcgtg ctgcccaagc tgtcgctgtg agcgcatgga     3120

ggcctgcatg ctcaatggca ctgtcattgg gcccgggaag actgtgatga tcgatgtgtg     3180

cacgacctgc cgctgcatgg tgcaggtggg ggtcatctct ggattcaagc tggagtgcag     3240

gaagaccacc tgcaacccct gccccctggg ttacaaggaa gaaaataaca caggtgaatg     3300

ttgtgggaga tgtttgccta cggcttgcac cattcagcta agaggaggac agatcatgac     3360

actgaagcgt gatgagacgc tccaggatgg ctgtgatact cacttctgca aggtcaatga     3420

gagaggagag tacttctggg agaagagggt cacaggctgc ccaccctttg atgaacacaa     3480

gtgtctggct gagggaggta aaattatgaa aattccaggc acctgctgtg acacatgtga     3540

ggagcctgag tgcaacgaca tcactgccag gctgcagtat gtcaaggtgg gaagctgtaa     3600

gtctgaagta gaggtggata tccactactg ccagggcaaa tgtgccagca aagccatgta     3660

ctccattgac atcaacgatg tgcaggacca gtgctcctgc tgctctccga cacggacgga     3720

gcccatgcag gtggccctgc actgcaccaa tggctctgtt gtgtaccatg aggttctcaa     3780

tgccatggag tgcaaatgct cccccaggaa gtgcagcaag tgactgactg aaacttgttt     3840

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca     3900

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc     3960

tggatcgtaa gttcctttct gttgactttg aaagaaaggt tagagatgtg tttggggctc     4020

ttgttcccac tggttaattt ttcctccttt ggtcttagtc cagtgcttcc ttttactatt     4080

atcttgtttt tgcgggtcca tctgtacatc ttgtgttttg cttcctgtct catgtacagg     4140

gggcctcctt gctgtgtagg cctgtgttca attctagggg tcagttgtct ggcagatggg     4200

cttagagttg gagtacctca tcttattccc tgcctgaatc tgctgttttc ttctgcagcc     4260

cggggacgtc tggaccttgc cagaccagtg ccacaccgtg acttgccagc cagatggcca     4320

gaccttgctg aagagtcatc gggtcaactg tgaccggggg ctgaggcctt cgtgccctaa     4380

cagccagtcc cctgttaaag tggaagagac ctgtggctgc cgctggacct gcccctgtga     4440

gtcctttgct tctccagcca gggcagcgtc aaaggggcag tgcttttagc ttggctgggc     4500

agaaaagtag agcaggcacc caccagccca gaagtaccct ttccctcatc accacatgca     4560

cagtgctacc ttcactcacc ttcctttcct cctgtgctct ttggacatgc atgcagccag     4620

tctcagggat cactgccctc tttctctgtc tttggaaggc acttccccag attatgcata     4680

actggaagga agaattgctt                                                 4700


<210>  17
<211>  4900
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct

<400>  17
acaaaggata aggcaaatac ttaacctctt tgtatatcat ctatacaaag gatacacatc       60

tttgtgtatc ctcatctata aaatggggat aataatagca cctacttgct taaaatagta      120

tctggcacat gacaagtgct caaaaaaaaa tgcttgctcc cactgctgtt actactacct      180

tttactgaca ctggcgtcta atccattcct agttcctgaa catctttatt cggtgtgttt      240

gggaccatcc cagaataata agccttcttt tagtatcttt tgagtcacac acttgtcagt      300

acttttgttt ctttgttttt gtttttattc acggccatag atttatttaa attcttgtaa      360

tatttctgct gaggaaaaca acaattacat catttcatca aatctcagat gtgctcagac      420

actaacagga gcactaggca tttatagctg gaagaatcac agtatgttca cctgccctgc      480

aagatctgag gacacagcag ctcattgtcc agggaggggc tgccgctcca tttccttttg      540

cagtctcctt gtattgccag gccagtattt tactcatttc tagaagaatg gtggcccctt      600

cttaccgagg aagcctatgc ctgctgcttt tatttgtaga catttaaact tcctttgggt      660

agaattggag tcttctcagt gtctctaaat ctgaggtagt ccggacccag gaactccatc      720

tccccatccc ctcctccctg gcccacattg cccttgtact cacgaaggca ccccccgccc      780

cccttggtgg tgccacgtgg tcagcacgcc ctgcagatcc tattggatgt caggttgtag      840

gcctggtggc cattgtccct gctggcacct gtgtgctcac cttcctggtt gtctttgcag      900

actgcagcca gcccctggac gtgatccttc tcctggatgg ctcctccagt ttcccagctt      960

cttattttga tgaaatgaag agtttcgcaa aagctttcat ttcaaaagcc aatatagggc     1020

ctcgtctcac tcaggtgtca gtgctgcagt atggaagcat caccaccatt gacgtgccat     1080

ggaacgtggt cccggagaaa gcccatttgc tgagccttgt ggacgtcatg cagcgggagg     1140

gaggccccag ccaaatcggg gatgccttgg gctttgctgt gcgatacttg acttcagaaa     1200

tgcatggtgc caggccggga gcctcaaagg cggtggtcat cctggtcacg gacgtctctg     1260

tggattcagt ggatgcagca gctgatgccg ccaggtccaa cagagtgaca gtgttcccta     1320

ttggaattgg agatcgctac gatgcagccc agctacggat cttggcaggc ccagcaggcg     1380

actccaacgt ggtgaagctc cagcgaatcg aagacctccc taccatggtc accttgggca     1440

attccttcct ccacaaactg tgctctggat ttgttaggat ttgcatggat gaggatggga     1500

atgagaagag gcccggggac gtctggacct tgccagacca gtgccacacc gtgacttgcc     1560

agccagatgg ccagaccttg ctgaagagtc atcgggtcaa ctgtgaccgg gggctgaggc     1620

cttcgtgccc taacagccag tcccctgtta aagtggaaga gacctgtggc tgccgctgga     1680

cctgcccctg cgtgtgcaca ggcagctcca ctcggcacat cgtgaccttt gatgggcaga     1740

atttcaagct gactggcagc tgttcttatg tcctatttca aaacaaggag caggacctgg     1800

aggtgattct ccataatggt gcctgcagcc ctggagcaag gcagggctgc atgaaatcca     1860

tcgaggtgaa gcacagtgcc ctctccgtcg agctgcacag tgacatggag gtgacggtga     1920

atgggagact ggtctctgtt ccttacgtgg gtgggaacat ggaagtcaac gtttatggtg     1980

ccatcatgca tgaggtcaga ttcaatcacc ttggtcacat cttcacattc actccacaaa     2040

acaatgagtt ccaactgcag ctcagcccca agacttttgc ttcaaagacg tatggtctgt     2100

gtgggatctg tgatgagaac ggagccaatg acttcatgct gagggatggc acagtcacca     2160

cagactggaa aacacttgtt caggaatgga ctgtgcagcg gccagggcag acgtgccagc     2220

ccatcctgga ggagcagtgt cttgtccccg acagctccca ctgccaggtc ctcctcttac     2280

cactgtttgc tgaatgccac aaggtcctgg ctccagccac attctatgcc atctgccagc     2340

aggacagttg ccaccaggag caagtgtgtg aggtgatcgc ctcttatgcc cacctctgtc     2400

ggaccaacgg ggtctgcgtt gactggagga cacctgattt ctgtgctatg tcatgcccac     2460

catctctggt ctacaaccac tgtgagcatg gctgtccccg gcactgtgat ggcaacgtga     2520

gctcctgtgg ggaccatccc tccgaaggct gtttctgccc tccagataaa gtcatgttgg     2580

aaggcagctg tgtccctgaa gaggcctgca ctcagtgcat tggtgaggat ggagtccagc     2640

accagttcct ggaagcctgg gtcccggacc accagccctg tcagatctgc acatgcctca     2700

gcgggcggaa ggtcaactgc acaacgcagc cctgccccac ggccaaagct cccacgtgtg     2760

gcctgtgtga agtagcccgc ctccgccaga atgcagacca gtgctgcccc gagtatgagt     2820

gtgtgtgtga cccagtgagc tgtgacctgc ccccagtgcc tcactgtgaa cgtggcctcc     2880

agcccacact gaccaaccct ggcgagtgca gacccaactt cacctgcgcc tgcaggaagg     2940

aggagtgcaa aagagtgtcc ccaccctcct gccccccgca ccgtttgccc acccttcgga     3000

agacccagtg ctgtgatgag tatgagtgtg cctgcaactg tgtcaactcc acagtgagct     3060

gtccccttgg gtacttggcc tcaactgcca ccaatgactg tggctgtacc acaaccacct     3120

gccttcccga caaggtgtgt gtccaccgaa gcaccatcta ccctgtgggc cagttctggg     3180

aggagggctg cgatgtgtgc acctgcaccg acatggagga tgccgtgatg ggcctccgcg     3240

tggcccagtg ctcccagaag ccctgtgagg acagctgtcg gtcgggcttc acttacgttc     3300

tgcatgaagg cgagtgctgt ggaaggtgcc tgccatctgc ctgtgaggtg gtgactggct     3360

caccgcgggg ggactcccag tcttcctgga agagtgtcgg ctcccagtgg gcctccccgg     3420

agaacccctg cctcatcaat gagtgtgtcc gagtgaagga ggaggtcttt atacaacaaa     3480

ggaacgtctc ctgcccccag ctggaggtcc ctgtctgccc ctcgggcttt cagctgagct     3540

gtaagacctc agcgtgctgc ccaagctgtc gctgtgagcg catggaggcc tgcatgctca     3600

atggcactgt cattgggccc gggaagactg tgatgatcga tgtgtgcacg acctgccgct     3660

gcatggtgca ggtgggggtc atctctggat tcaagctgga gtgcaggaag accacctgca     3720

acccctgccc cctgggttac aaggaagaaa ataacacagg tgaatgttgt gggagatgtt     3780

tgcctacggc ttgcaccatt cagctaagag gaggacagat catgacactg aagcgtgatg     3840

agacgctcca ggatggctgt gatactcact tctgcaaggt caatgagaga ggagagtact     3900

tctgggagaa gagggtcaca ggctgcccac cctttgatga acacaagtgt ctggctgagg     3960

gaggtaaaat tatgaaaatt ccaggcacct gctgtgacac atgtgaggag cctgagtgca     4020

acgacatcac tgccaggctg cagtatgtca aggtgggaag ctgtaagtct gaagtagagg     4080

tggatatcca ctactgccag ggcaaatgtg ccagcaaagc catgtactcc attgacatca     4140

acgatgtgca ggaccagtgc tcctgctgct ctccgacacg gacggagccc atgcaggtgg     4200

ccctgcactg caccaatggc tctgttgtgt accatgaggt tctcaatgcc atggagtgca     4260

aatgctcccc caggaagtgc agcaagtgac tgactgaaac ttgtttattg cagcttataa     4320

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca     4380

ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgtgggtga     4440

gcgaggcacc tgaagcagca ggtgacgaag aggctctttt tgtggctcta cttgattcaa     4500

aataatccgc attttctcgt tccgtttagg gcctcgtctc actcaggtgt cagtgctgca     4560

gtatggaagc atcaccacca ttgacgtgcc atggaacgtg gtcccggaga aagcccattt     4620

gctgagcctt gtggacgtca tgcagcggga gggaggcccc agccaaatcg gtaacgttgg     4680

tgccacaggc tggatgcaga agctgcattc tggttcttat ttttggcata agtgactgtg     4740

tgacctcggc cagtcacttt gctccttggc cttagtttct tctcctggaa agtgaggggc     4800

tagatgctct tccacgtctc tccagatctc aactgggtgt tccttggagt ttctgaatca     4860

ttcagctttt aagtgactta aggatccacc gttaagacag                           4900


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target site

<400>  18
aaaggtcacg atgtgccgag                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target site

<400>  19
ggatttgcat ggatgaggat                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR target site

<400>  20
tgaaatgaag agtttcgcca                                                   20


<210>  21
<211>  639
<212>  PRT
<213>  Scytonema hoffmanni

<400>  21

Met Ser Gln Ile Thr Ile Gln Ala Arg Leu Ile Ser Phe Glu Ser Asn 
1               5                   10                  15      


Arg Gln Gln Leu Trp Lys Leu Met Ala Asp Leu Asn Thr Pro Leu Ile 
            20                  25                  30          


Asn Glu Leu Leu Cys Gln Leu Gly Gln His Pro Asp Phe Glu Lys Trp 
        35                  40                  45              


Gln Gln Lys Gly Lys Leu Pro Ser Thr Val Val Ser Gln Leu Cys Gln 
    50                  55                  60                  


Pro Leu Lys Thr Asp Pro Arg Phe Ala Gly Gln Pro Ser Arg Leu Tyr 
65                  70                  75                  80  


Met Ser Ala Ile His Ile Val Asp Tyr Ile Tyr Lys Ser Trp Leu Ala 
                85                  90                  95      


Ile Gln Lys Arg Leu Gln Gln Gln Leu Asp Gly Lys Thr Arg Trp Leu 
            100                 105                 110         


Glu Met Leu Asn Ser Asp Ala Glu Leu Val Glu Leu Ser Gly Asp Thr 
        115                 120                 125             


Leu Glu Ala Ile Arg Val Lys Ala Ala Glu Ile Leu Ala Ile Ala Met 
    130                 135                 140                 


Pro Ala Ser Glu Ser Asp Ser Ala Ser Pro Lys Gly Lys Lys Gly Lys 
145                 150                 155                 160 


Lys Glu Lys Lys Pro Ser Ser Ser Ser Pro Lys Arg Ser Leu Ser Lys 
                165                 170                 175     


Thr Leu Phe Asp Ala Tyr Gln Glu Thr Glu Asp Ile Lys Ser Arg Ser 
            180                 185                 190         


Ala Ile Ser Tyr Leu Leu Lys Asn Gly Cys Lys Leu Thr Asp Lys Glu 
        195                 200                 205             


Glu Asp Ser Glu Lys Phe Ala Lys Arg Arg Arg Gln Val Glu Ile Gln 
    210                 215                 220                 


Ile Gln Arg Leu Thr Glu Lys Leu Ile Ser Arg Met Pro Lys Gly Arg 
225                 230                 235                 240 


Asp Leu Thr Asn Ala Lys Trp Leu Glu Thr Leu Leu Thr Ala Thr Thr 
                245                 250                 255     


Thr Val Ala Glu Asp Asn Ala Gln Ala Lys Arg Trp Gln Asp Ile Leu 
            260                 265                 270         


Leu Thr Arg Ser Ser Ser Leu Pro Phe Pro Leu Val Phe Glu Thr Asn 
        275                 280                 285             


Glu Asp Met Val Trp Ser Lys Asn Gln Lys Gly Arg Leu Cys Val His 
    290                 295                 300                 


Phe Asn Gly Leu Ser Asp Leu Ile Phe Glu Val Tyr Cys Gly Asn Arg 
305                 310                 315                 320 


Gln Leu His Trp Phe Gln Arg Phe Leu Glu Asp Gln Gln Thr Lys Arg 
                325                 330                 335     


Lys Ser Lys Asn Gln His Ser Ser Gly Leu Phe Thr Leu Arg Asn Gly 
            340                 345                 350         


His Leu Val Trp Leu Glu Gly Glu Gly Lys Gly Glu Pro Trp Asn Leu 
        355                 360                 365             


His His Leu Thr Leu Tyr Cys Cys Val Asp Asn Arg Leu Trp Thr Glu 
    370                 375                 380                 


Glu Gly Thr Glu Ile Val Arg Gln Glu Lys Ala Asp Glu Ile Thr Lys 
385                 390                 395                 400 


Phe Ile Thr Asn Met Lys Lys Lys Ser Asp Leu Ser Asp Thr Gln Gln 
                405                 410                 415     


Ala Leu Ile Gln Arg Lys Gln Ser Thr Leu Thr Arg Ile Asn Asn Ser 
            420                 425                 430         


Phe Glu Arg Pro Ser Gln Pro Leu Tyr Gln Gly Gln Ser His Ile Leu 
        435                 440                 445             


Val Gly Val Ser Leu Gly Leu Glu Lys Pro Ala Thr Val Ala Val Val 
    450                 455                 460                 


Asp Ala Ile Ala Asn Lys Val Leu Ala Tyr Arg Ser Ile Lys Gln Leu 
465                 470                 475                 480 


Leu Gly Asp Asn Tyr Glu Leu Leu Asn Arg Gln Arg Arg Gln Gln Gln 
                485                 490                 495     


Tyr Leu Ser His Glu Arg His Lys Ala Gln Lys Asn Phe Ser Pro Asn 
            500                 505                 510         


Gln Phe Gly Ala Ser Glu Leu Gly Gln His Ile Asp Arg Leu Leu Ala 
        515                 520                 525             


Lys Ala Ile Val Ala Leu Ala Arg Thr Tyr Lys Ala Gly Ser Ile Val 
    530                 535                 540                 


Leu Pro Lys Leu Gly Asp Met Arg Glu Val Val Gln Ser Glu Ile Gln 
545                 550                 555                 560 


Ala Ile Ala Glu Gln Lys Phe Pro Gly Tyr Ile Glu Gly Gln Gln Lys 
                565                 570                 575     


Tyr Ala Lys Gln Tyr Arg Val Asn Val His Arg Trp Ser Tyr Gly Arg 
            580                 585                 590         


Leu Ile Gln Ser Ile Gln Ser Lys Ala Ala Gln Thr Gly Ile Val Ile 
        595                 600                 605             


Glu Glu Gly Lys Gln Pro Ile Arg Gly Ser Pro His Asp Lys Ala Lys 
    610                 615                 620                 


Glu Leu Ala Leu Ser Ala Tyr Asn Leu Arg Leu Thr Arg Arg Ser 
625                 630                 635                 


<210>  22
<211>  642
<212>  PRT
<213>  Anabaena cylindrica

<400>  22

Met Ser Val Ile Thr Ile Gln Cys Arg Leu Val Ala Glu Glu Asp Ser 
1               5                   10                  15      


Leu Arg Gln Leu Trp Glu Leu Met Ser Glu Lys Asn Thr Pro Phe Ile 
            20                  25                  30          


Asn Glu Ile Leu Leu Gln Ile Gly Lys His Pro Glu Phe Glu Thr Trp 
        35                  40                  45              


Leu Glu Lys Gly Arg Ile Pro Ala Glu Leu Leu Lys Thr Leu Gly Asn 
    50                  55                  60                  


Ser Leu Lys Thr Gln Glu Pro Phe Thr Gly Gln Pro Gly Arg Phe Tyr 
65                  70                  75                  80  


Thr Ser Ala Ile Thr Leu Val Asp Tyr Leu Tyr Lys Ser Trp Phe Ala 
                85                  90                  95      


Leu Gln Lys Arg Arg Lys Gln Gln Ile Glu Gly Lys Gln Arg Trp Leu 
            100                 105                 110         


Lys Met Leu Lys Ser Asp Gln Glu Leu Glu Gln Glu Ser Gln Ser Ser 
        115                 120                 125             


Leu Glu Val Ile Arg Asn Lys Ala Thr Glu Leu Phe Ser Lys Phe Thr 
    130                 135                 140                 


Pro Gln Ser Asp Ser Glu Ala Leu Arg Arg Asn Gln Asn Asp Lys Gln 
145                 150                 155                 160 


Lys Lys Val Lys Lys Thr Lys Lys Ser Thr Lys Pro Lys Thr Ser Ser 
                165                 170                 175     


Ile Phe Lys Ile Phe Leu Ser Thr Tyr Glu Glu Ala Glu Glu Pro Leu 
            180                 185                 190         


Thr Arg Cys Ala Leu Ala Tyr Leu Leu Lys Asn Asn Cys Gln Ile Ser 
        195                 200                 205             


Glu Leu Asp Glu Asn Pro Glu Glu Phe Thr Arg Asn Lys Arg Arg Lys 
    210                 215                 220                 


Glu Ile Glu Ile Glu Arg Leu Lys Asp Gln Leu Gln Ser Arg Ile Pro 
225                 230                 235                 240 


Lys Gly Arg Asp Leu Thr Gly Glu Glu Trp Leu Glu Thr Leu Glu Ile 
                245                 250                 255     


Ala Thr Phe Asn Val Pro Gln Asn Glu Asn Glu Ala Lys Ala Trp Gln 
            260                 265                 270         


Ala Ala Leu Leu Arg Lys Thr Ala Asn Val Pro Phe Pro Val Ala Tyr 
        275                 280                 285             


Glu Ser Asn Glu Asp Met Thr Trp Leu Lys Asn Asp Lys Asn Arg Leu 
    290                 295                 300                 


Phe Val Arg Phe Asn Gly Leu Gly Lys Leu Thr Phe Glu Ile Tyr Cys 
305                 310                 315                 320 


Asp Lys Arg His Leu His Tyr Phe Gln Arg Phe Leu Glu Asp Gln Glu 
                325                 330                 335     


Ile Leu Arg Asn Ser Lys Arg Gln His Ser Ser Ser Leu Phe Thr Leu 
            340                 345                 350         


Arg Ser Gly Arg Ile Ala Trp Leu Pro Gly Glu Glu Lys Gly Glu His 
        355                 360                 365             


Trp Lys Val Asn Gln Leu Asn Phe Tyr Cys Ser Leu Asp Thr Arg Met 
    370                 375                 380                 


Leu Thr Thr Glu Gly Thr Gln Gln Val Val Glu Glu Lys Val Thr Ala 
385                 390                 395                 400 


Ile Thr Glu Ile Leu Asn Lys Thr Lys Gln Lys Asp Asp Leu Asn Asp 
                405                 410                 415     


Lys Gln Gln Ala Phe Ile Thr Arg Gln Gln Ser Thr Leu Ala Arg Ile 
            420                 425                 430         


Asn Asn Pro Phe Pro Arg Pro Ser Lys Pro Asn Tyr Gln Gly Lys Ser 
        435                 440                 445             


Ser Ile Leu Ile Gly Val Ser Phe Gly Leu Glu Lys Pro Val Thr Val 
    450                 455                 460                 


Ala Val Val Asp Val Val Lys Asn Lys Val Ile Ala Tyr Arg Ser Val 
465                 470                 475                 480 


Lys Gln Leu Leu Gly Glu Asn Tyr Asn Leu Leu Asn Arg Gln Arg Gln 
                485                 490                 495     


Gln Gln Gln Arg Leu Ser His Glu Arg His Lys Ala Gln Lys Gln Asn 
            500                 505                 510         


Ala Pro Asn Ser Phe Gly Glu Ser Glu Leu Gly Gln Tyr Val Asp Arg 
        515                 520                 525             


Leu Leu Ala Asp Ala Ile Ile Ala Ile Ala Lys Lys Tyr Gln Ala Gly 
    530                 535                 540                 


Ser Ile Val Leu Pro Lys Leu Arg Asp Met Arg Glu Gln Ile Ser Ser 
545                 550                 555                 560 


Glu Ile Gln Ser Arg Ala Glu Asn Gln Cys Pro Gly Tyr Lys Glu Gly 
                565                 570                 575     


Gln Gln Lys Tyr Ala Lys Glu Tyr Arg Ile Asn Val His Arg Trp Ser 
            580                 585                 590         


Tyr Gly Arg Leu Ile Glu Ser Ile Lys Ser Gln Ala Ala Gln Ala Gly 
        595                 600                 605             


Ile Ala Ile Glu Thr Gly Lys Gln Ser Ile Arg Gly Ser Pro Gln Glu 
    610                 615                 620                 


Lys Ala Arg Asp Leu Ala Val Phe Thr Tyr Gln Glu Arg Gln Ala Ala 
625                 630                 635                 640 


Leu Ile 
        


<210>  23
<211>  208
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left end for ShCas12k

<400>  23
tacagtgaca aattatctgt cgtcggtgac agattaatgt cattgtgact atttaattgt       60

cgtcgtgacc catcagcgtt gcttaattaa ttgatgacaa attaaatgtc atcaatataa      120

tatgctctgc aattattata caaagcaatt aaaacaagcg gataaaagga cttgctttca      180

acccacccct aagtttaata gttactga                                         208


<210>  24
<211>  219
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right end for ShCas12k

<400>  24
cgacagtcaa tttgtcatta tgaaaataca caaaagcttt ttcctatctt gcaaagcgac       60

agctaatttg tcacaatcac ggacaacgac atctattttg tcactgcaaa gaggttatgc      120

taaaactgcc aaagcgctat aatctatact gtataaggat tttactgatg acaataattt      180

gtcacaacga catataatta gtcactgtac acgtagaga                             219


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  25
tgtatttctg ttcagggaga tgg                                               23


<210>  26
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  26
agatgtactg ccaagtagga aag                                               23


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  27
ccatcacacc atgtgctact                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  28
tccattcaga ccacaccaag                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  29
gggatgggag gtgaattctt                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  30
gggatgggag gtgaattctt                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  31
acgttctggt gcaggattac                                                   20


<210>  32
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  32
tggcccatga ctcaatgata ag                                                22


<210>  33
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  33
ccgatagaac tttctgcagt gg                                                22


<210>  34
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  34
ctgtagaatc cttaccagtg acg                                               23


<210>  35
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  35
cctgccacct tgactatgg                                                    19


<210>  36
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  36
tatgcagagg agataggaga gg                                                22


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  37
gatcccacac agaccatacg                                                   20


<210>  38
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  38
gcattctagt tgtggtttgt cc                                                22


<210>  39
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  39
gtgtctccaa gagcatctag c                                                 21


<210>  40
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  40
gtgcccatgc ataagatttg g                                                 21


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  41
ccagtcagct tgaaattctg c                                                 21


<210>  42
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  42
tgttcagcat aaaggttaca atcc                                              24


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  43
gatgtcaggt gtcaggtagc                                                   20


<210>  44
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  44
atgatcactc ctggacacaa ag                                                22


<210>  45
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAST target site

<400>  45
gggctgggaa gtcagtcccg ctc                                               23


<210>  46
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAST target site

<400>  46
gaattgatcc ctttaccatt atg                                               23


<210>  47
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAST target site

<400>  47
tgaagtgatg aatcttattg ctt                                               23


<210>  48
<211>  2813
<212>  PRT
<213>  Homo sapiens

<400>  48

Met Ile Pro Ala Arg Phe Ala Gly Val Leu Leu Ala Leu Ala Leu Ile 
1               5                   10                  15      


Leu Pro Gly Thr Leu Cys Ala Glu Gly Thr Arg Gly Arg Ser Ser Thr 
            20                  25                  30          


Ala Arg Cys Ser Leu Phe Gly Ser Asp Phe Val Asn Thr Phe Asp Gly 
        35                  40                  45              


Ser Met Tyr Ser Phe Ala Gly Tyr Cys Ser Tyr Leu Leu Ala Gly Gly 
    50                  55                  60                  


Cys Gln Lys Arg Ser Phe Ser Ile Ile Gly Asp Phe Gln Asn Gly Lys 
65                  70                  75                  80  


Arg Val Ser Leu Ser Val Tyr Leu Gly Glu Phe Phe Asp Ile His Leu 
                85                  90                  95      


Phe Val Asn Gly Thr Val Thr Gln Gly Asp Gln Arg Val Ser Met Pro 
            100                 105                 110         


Tyr Ala Ser Lys Gly Leu Tyr Leu Glu Thr Glu Ala Gly Tyr Tyr Lys 
        115                 120                 125             


Leu Ser Gly Glu Ala Tyr Gly Phe Val Ala Arg Ile Asp Gly Ser Gly 
    130                 135                 140                 


Asn Phe Gln Val Leu Leu Ser Asp Arg Tyr Phe Asn Lys Thr Cys Gly 
145                 150                 155                 160 


Leu Cys Gly Asn Phe Asn Ile Phe Ala Glu Asp Asp Phe Met Thr Gln 
                165                 170                 175     


Glu Gly Thr Leu Thr Ser Asp Pro Tyr Asp Phe Ala Asn Ser Trp Ala 
            180                 185                 190         


Leu Ser Ser Gly Glu Gln Trp Cys Glu Arg Ala Ser Pro Pro Ser Ser 
        195                 200                 205             


Ser Cys Asn Ile Ser Ser Gly Glu Met Gln Lys Gly Leu Trp Glu Gln 
    210                 215                 220                 


Cys Gln Leu Leu Lys Ser Thr Ser Val Phe Ala Arg Cys His Pro Leu 
225                 230                 235                 240 


Val Asp Pro Glu Pro Phe Val Ala Leu Cys Glu Lys Thr Leu Cys Glu 
                245                 250                 255     


Cys Ala Gly Gly Leu Glu Cys Ala Cys Pro Ala Leu Leu Glu Tyr Ala 
            260                 265                 270         


Arg Thr Cys Ala Gln Glu Gly Met Val Leu Tyr Gly Trp Thr Asp His 
        275                 280                 285             


Ser Ala Cys Ser Pro Val Cys Pro Ala Gly Met Glu Tyr Arg Gln Cys 
    290                 295                 300                 


Val Ser Pro Cys Ala Arg Thr Cys Gln Ser Leu His Ile Asn Glu Met 
305                 310                 315                 320 


Cys Gln Glu Arg Cys Val Asp Gly Cys Ser Cys Pro Glu Gly Gln Leu 
                325                 330                 335     


Leu Asp Glu Gly Leu Cys Val Glu Ser Thr Glu Cys Pro Cys Val His 
            340                 345                 350         


Ser Gly Lys Arg Tyr Pro Pro Gly Thr Ser Leu Ser Arg Asp Cys Asn 
        355                 360                 365             


Thr Cys Ile Cys Arg Asn Ser Gln Trp Ile Cys Ser Asn Glu Glu Cys 
    370                 375                 380                 


Pro Gly Glu Cys Leu Val Thr Gly Gln Ser His Phe Lys Ser Phe Asp 
385                 390                 395                 400 


Asn Arg Tyr Phe Thr Phe Ser Gly Ile Cys Gln Tyr Leu Leu Ala Arg 
                405                 410                 415     


Asp Cys Gln Asp His Ser Phe Ser Ile Val Ile Glu Thr Val Gln Cys 
            420                 425                 430         


Ala Asp Asp Arg Asp Ala Val Cys Thr Arg Ser Val Thr Val Arg Leu 
        435                 440                 445             


Pro Gly Leu His Asn Ser Leu Val Lys Leu Lys His Gly Ala Gly Val 
    450                 455                 460                 


Ala Met Asp Gly Gln Asp Val Gln Leu Pro Leu Leu Lys Gly Asp Leu 
465                 470                 475                 480 


Arg Ile Gln His Thr Val Thr Ala Ser Val Arg Leu Ser Tyr Gly Glu 
                485                 490                 495     


Asp Leu Gln Met Asp Trp Asp Gly Arg Gly Arg Leu Leu Val Lys Leu 
            500                 505                 510         


Ser Pro Val Tyr Ala Gly Lys Thr Cys Gly Leu Cys Gly Asn Tyr Asn 
        515                 520                 525             


Gly Asn Gln Gly Asp Asp Phe Leu Thr Pro Ser Gly Leu Ala Glu Pro 
    530                 535                 540                 


Arg Val Glu Asp Phe Gly Asn Ala Trp Lys Leu His Gly Asp Cys Gln 
545                 550                 555                 560 


Asp Leu Gln Lys Gln His Ser Asp Pro Cys Ala Leu Asn Pro Arg Met 
                565                 570                 575     


Thr Arg Phe Ser Glu Glu Ala Cys Ala Val Leu Thr Ser Pro Thr Phe 
            580                 585                 590         


Glu Ala Cys His Arg Ala Val Ser Pro Leu Pro Tyr Leu Arg Asn Cys 
        595                 600                 605             


Arg Tyr Asp Val Cys Ser Cys Ser Asp Gly Arg Glu Cys Leu Cys Gly 
    610                 615                 620                 


Ala Leu Ala Ser Tyr Ala Ala Ala Cys Ala Gly Arg Gly Val Arg Val 
625                 630                 635                 640 


Ala Trp Arg Glu Pro Gly Arg Cys Glu Leu Asn Cys Pro Lys Gly Gln 
                645                 650                 655     


Val Tyr Leu Gln Cys Gly Thr Pro Cys Asn Leu Thr Cys Arg Ser Leu 
            660                 665                 670         


Ser Tyr Pro Asp Glu Glu Cys Asn Glu Ala Cys Leu Glu Gly Cys Phe 
        675                 680                 685             


Cys Pro Pro Gly Leu Tyr Met Asp Glu Arg Gly Asp Cys Val Pro Lys 
    690                 695                 700                 


Ala Gln Cys Pro Cys Tyr Tyr Asp Gly Glu Ile Phe Gln Pro Glu Asp 
705                 710                 715                 720 


Ile Phe Ser Asp His His Thr Met Cys Tyr Cys Glu Asp Gly Phe Met 
                725                 730                 735     


His Cys Thr Met Ser Gly Val Pro Gly Ser Leu Leu Pro Asp Ala Val 
            740                 745                 750         


Leu Ser Ser Pro Leu Ser His Arg Ser Lys Arg Ser Leu Ser Cys Arg 
        755                 760                 765             


Pro Pro Met Val Lys Leu Val Cys Pro Ala Asp Asn Leu Arg Ala Glu 
    770                 775                 780                 


Gly Leu Glu Cys Thr Lys Thr Cys Gln Asn Tyr Asp Leu Glu Cys Met 
785                 790                 795                 800 


Ser Met Gly Cys Val Ser Gly Cys Leu Cys Pro Pro Gly Met Val Arg 
                805                 810                 815     


His Glu Asn Arg Cys Val Ala Leu Glu Arg Cys Pro Cys Phe His Gln 
            820                 825                 830         


Gly Lys Glu Tyr Ala Pro Gly Glu Thr Val Lys Ile Gly Cys Asn Thr 
        835                 840                 845             


Cys Val Cys Gln Asp Arg Lys Trp Asn Cys Thr Asp His Val Cys Asp 
    850                 855                 860                 


Ala Thr Cys Ser Thr Ile Gly Met Ala His Tyr Leu Thr Phe Asp Gly 
865                 870                 875                 880 


Leu Lys Tyr Leu Phe Pro Gly Glu Cys Gln Tyr Val Leu Val Gln Asp 
                885                 890                 895     


Tyr Cys Gly Ser Asn Pro Gly Thr Phe Arg Ile Leu Val Gly Asn Lys 
            900                 905                 910         


Gly Cys Ser His Pro Ser Val Lys Cys Lys Lys Arg Val Thr Ile Leu 
        915                 920                 925             


Val Glu Gly Gly Glu Ile Glu Leu Phe Asp Gly Glu Val Asn Val Lys 
    930                 935                 940                 


Arg Pro Met Lys Asp Glu Thr His Phe Glu Val Val Glu Ser Gly Arg 
945                 950                 955                 960 


Tyr Ile Ile Leu Leu Leu Gly Lys Ala Leu Ser Val Val Trp Asp Arg 
                965                 970                 975     


His Leu Ser Ile Ser Val Val Leu Lys Gln Thr Tyr Gln Glu Lys Val 
            980                 985                 990         


Cys Gly Leu Cys Gly Asn Phe Asp  Gly Ile Gln Asn Asn  Asp Leu Thr 
        995                 1000                 1005             


Ser Ser  Asn Leu Gln Val Glu  Glu Asp Pro Val Asp  Phe Gly Asn 
    1010                 1015                 1020             


Ser Trp  Lys Val Ser Ser Gln  Cys Ala Asp Thr Arg  Lys Val Pro 
    1025                 1030                 1035             


Leu Asp  Ser Ser Pro Ala Thr  Cys His Asn Asn Ile  Met Lys Gln 
    1040                 1045                 1050             


Thr Met  Val Asp Ser Ser Cys  Arg Ile Leu Thr Ser  Asp Val Phe 
    1055                 1060                 1065             


Gln Asp  Cys Asn Lys Leu Val  Asp Pro Glu Pro Tyr  Leu Asp Val 
    1070                 1075                 1080             


Cys Ile  Tyr Asp Thr Cys Ser  Cys Glu Ser Ile Gly  Asp Cys Ala 
    1085                 1090                 1095             


Cys Phe  Cys Asp Thr Ile Ala  Ala Tyr Ala His Val  Cys Ala Gln 
    1100                 1105                 1110             


His Gly  Lys Val Val Thr Trp  Arg Thr Ala Thr Leu  Cys Pro Gln 
    1115                 1120                 1125             


Ser Cys  Glu Glu Arg Asn Leu  Arg Glu Asn Gly Tyr  Glu Cys Glu 
    1130                 1135                 1140             


Trp Arg  Tyr Asn Ser Cys Ala  Pro Ala Cys Gln Val  Thr Cys Gln 
    1145                 1150                 1155             


His Pro  Glu Pro Leu Ala Cys  Pro Val Gln Cys Val  Glu Gly Cys 
    1160                 1165                 1170             


His Ala  His Cys Pro Pro Gly  Lys Ile Leu Asp Glu  Leu Leu Gln 
    1175                 1180                 1185             


Thr Cys  Val Asp Pro Glu Asp  Cys Pro Val Cys Glu  Val Ala Gly 
    1190                 1195                 1200             


Arg Arg  Phe Ala Ser Gly Lys  Lys Val Thr Leu Asn  Pro Ser Asp 
    1205                 1210                 1215             


Pro Glu  His Cys Gln Ile Cys  His Cys Asp Val Val  Asn Leu Thr 
    1220                 1225                 1230             


Cys Glu  Ala Cys Gln Glu Pro  Gly Gly Leu Val Val  Pro Pro Thr 
    1235                 1240                 1245             


Asp Ala  Pro Val Ser Pro Thr  Thr Leu Tyr Val Glu  Asp Ile Ser 
    1250                 1255                 1260             


Glu Pro  Pro Leu His Asp Phe  Tyr Cys Ser Arg Leu  Leu Asp Leu 
    1265                 1270                 1275             


Val Phe  Leu Leu Asp Gly Ser  Ser Arg Leu Ser Glu  Ala Glu Phe 
    1280                 1285                 1290             


Glu Val  Leu Lys Ala Phe Val  Val Asp Met Met Glu  Arg Leu Arg 
    1295                 1300                 1305             


Ile Ser  Gln Lys Trp Val Arg  Val Ala Val Val Glu  Tyr His Asp 
    1310                 1315                 1320             


Gly Ser  His Ala Tyr Ile Gly  Leu Lys Asp Arg Lys  Arg Pro Ser 
    1325                 1330                 1335             


Glu Leu  Arg Arg Ile Ala Ser  Gln Val Lys Tyr Ala  Gly Ser Gln 
    1340                 1345                 1350             


Val Ala  Ser Thr Ser Glu Val  Leu Lys Tyr Thr Leu  Phe Gln Ile 
    1355                 1360                 1365             


Phe Ser  Lys Ile Asp Arg Pro  Glu Ala Ser Arg Ile  Thr Leu Leu 
    1370                 1375                 1380             


Leu Met  Ala Ser Gln Glu Pro  Gln Arg Met Ser Arg  Asn Phe Val 
    1385                 1390                 1395             


Arg Tyr  Val Gln Gly Leu Lys  Lys Lys Lys Val Ile  Val Ile Pro 
    1400                 1405                 1410             


Val Gly  Ile Gly Pro His Ala  Asn Leu Lys Gln Ile  Arg Leu Ile 
    1415                 1420                 1425             


Glu Lys  Gln Ala Pro Glu Asn  Lys Ala Phe Val Leu  Ser Ser Val 
    1430                 1435                 1440             


Asp Glu  Leu Glu Gln Gln Arg  Asp Glu Ile Val Ser  Tyr Leu Cys 
    1445                 1450                 1455             


Asp Leu  Ala Pro Glu Ala Pro  Pro Pro Thr Leu Pro  Pro Asp Met 
    1460                 1465                 1470             


Ala Gln  Val Thr Val Gly Pro  Gly Leu Leu Gly Val  Ser Thr Leu 
    1475                 1480                 1485             


Gly Pro  Lys Arg Asn Ser Met  Val Leu Asp Val Ala  Phe Val Leu 
    1490                 1495                 1500             


Glu Gly  Ser Asp Lys Ile Gly  Glu Ala Asp Phe Asn  Arg Ser Lys 
    1505                 1510                 1515             


Glu Phe  Met Glu Glu Val Ile  Gln Arg Met Asp Val  Gly Gln Asp 
    1520                 1525                 1530             


Ser Ile  His Val Thr Val Leu  Gln Tyr Ser Tyr Met  Val Thr Val 
    1535                 1540                 1545             


Glu Tyr  Pro Phe Ser Glu Ala  Gln Ser Lys Gly Asp  Ile Leu Gln 
    1550                 1555                 1560             


Arg Val  Arg Glu Ile Arg Tyr  Gln Gly Gly Asn Arg  Thr Asn Thr 
    1565                 1570                 1575             


Gly Leu  Ala Leu Arg Tyr Leu  Ser Asp His Ser Phe  Leu Val Ser 
    1580                 1585                 1590             


Gln Gly  Asp Arg Glu Gln Ala  Pro Asn Leu Val Tyr  Met Val Thr 
    1595                 1600                 1605             


Gly Asn  Pro Ala Ser Asp Glu  Ile Lys Arg Leu Pro  Gly Asp Ile 
    1610                 1615                 1620             


Gln Val  Val Pro Ile Gly Val  Gly Pro Asn Ala Asn  Val Gln Glu 
    1625                 1630                 1635             


Leu Glu  Arg Ile Gly Trp Pro  Asn Ala Pro Ile Leu  Ile Gln Asp 
    1640                 1645                 1650             


Phe Glu  Thr Leu Pro Arg Glu  Ala Pro Asp Leu Val  Leu Gln Arg 
    1655                 1660                 1665             


Cys Cys  Ser Gly Glu Gly Leu  Gln Ile Pro Thr Leu  Ser Pro Ala 
    1670                 1675                 1680             


Pro Asp  Cys Ser Gln Pro Leu  Asp Val Ile Leu Leu  Leu Asp Gly 
    1685                 1690                 1695             


Ser Ser  Ser Phe Pro Ala Ser  Tyr Phe Asp Glu Met  Lys Ser Phe 
    1700                 1705                 1710             


Ala Lys  Ala Phe Ile Ser Lys  Ala Asn Ile Gly Pro  Arg Leu Thr 
    1715                 1720                 1725             


Gln Val  Ser Val Leu Gln Tyr  Gly Ser Ile Thr Thr  Ile Asp Val 
    1730                 1735                 1740             


Pro Trp  Asn Val Val Pro Glu  Lys Ala His Leu Leu  Ser Leu Val 
    1745                 1750                 1755             


Asp Val  Met Gln Arg Glu Gly  Gly Pro Ser Gln Ile  Gly Asp Ala 
    1760                 1765                 1770             


Leu Gly  Phe Ala Val Arg Tyr  Leu Thr Ser Glu Met  His Gly Ala 
    1775                 1780                 1785             


Arg Pro  Gly Ala Ser Lys Ala  Val Val Ile Leu Val  Thr Asp Val 
    1790                 1795                 1800             


Ser Val  Asp Ser Val Asp Ala  Ala Ala Asp Ala Ala  Arg Ser Asn 
    1805                 1810                 1815             


Arg Val  Thr Val Phe Pro Ile  Gly Ile Gly Asp Arg  Tyr Asp Ala 
    1820                 1825                 1830             


Ala Gln  Leu Arg Ile Leu Ala  Gly Pro Ala Gly Asp  Ser Asn Val 
    1835                 1840                 1845             


Val Lys  Leu Gln Arg Ile Glu  Asp Leu Pro Thr Met  Val Thr Leu 
    1850                 1855                 1860             


Gly Asn  Ser Phe Leu His Lys  Leu Cys Ser Gly Phe  Val Arg Ile 
    1865                 1870                 1875             


Cys Met  Asp Glu Asp Gly Asn  Glu Lys Arg Pro Gly  Asp Val Trp 
    1880                 1885                 1890             


Thr Leu  Pro Asp Gln Cys His  Thr Val Thr Cys Gln  Pro Asp Gly 
    1895                 1900                 1905             


Gln Thr  Leu Leu Lys Ser His  Arg Val Asn Cys Asp  Arg Gly Leu 
    1910                 1915                 1920             


Arg Pro  Ser Cys Pro Asn Ser  Gln Ser Pro Val Lys  Val Glu Glu 
    1925                 1930                 1935             


Thr Cys  Gly Cys Arg Trp Thr  Cys Pro Cys Val Cys  Thr Gly Ser 
    1940                 1945                 1950             


Ser Thr  Arg His Ile Val Thr  Phe Asp Gly Gln Asn  Phe Lys Leu 
    1955                 1960                 1965             


Thr Gly  Ser Cys Ser Tyr Val  Leu Phe Gln Asn Lys  Glu Gln Asp 
    1970                 1975                 1980             


Leu Glu  Val Ile Leu His Asn  Gly Ala Cys Ser Pro  Gly Ala Arg 
    1985                 1990                 1995             


Gln Gly  Cys Met Lys Ser Ile  Glu Val Lys His Ser  Ala Leu Ser 
    2000                 2005                 2010             


Val Glu  Leu His Ser Asp Met  Glu Val Thr Val Asn  Gly Arg Leu 
    2015                 2020                 2025             


Val Ser  Val Pro Tyr Val Gly  Gly Asn Met Glu Val  Asn Val Tyr 
    2030                 2035                 2040             


Gly Ala  Ile Met His Glu Val  Arg Phe Asn His Leu  Gly His Ile 
    2045                 2050                 2055             


Phe Thr  Phe Thr Pro Gln Asn  Asn Glu Phe Gln Leu  Gln Leu Ser 
    2060                 2065                 2070             


Pro Lys  Thr Phe Ala Ser Lys  Thr Tyr Gly Leu Cys  Gly Ile Cys 
    2075                 2080                 2085             


Asp Glu  Asn Gly Ala Asn Asp  Phe Met Leu Arg Asp  Gly Thr Val 
    2090                 2095                 2100             


Thr Thr  Asp Trp Lys Thr Leu  Val Gln Glu Trp Thr  Val Gln Arg 
    2105                 2110                 2115             


Pro Gly  Gln Thr Cys Gln Pro  Ile Leu Glu Glu Gln  Cys Leu Val 
    2120                 2125                 2130             


Pro Asp  Ser Ser His Cys Gln  Val Leu Leu Leu Pro  Leu Phe Ala 
    2135                 2140                 2145             


Glu Cys  His Lys Val Leu Ala  Pro Ala Thr Phe Tyr  Ala Ile Cys 
    2150                 2155                 2160             


Gln Gln  Asp Ser Cys His Gln  Glu Gln Val Cys Glu  Val Ile Ala 
    2165                 2170                 2175             


Ser Tyr  Ala His Leu Cys Arg  Thr Asn Gly Val Cys  Val Asp Trp 
    2180                 2185                 2190             


Arg Thr  Pro Asp Phe Cys Ala  Met Ser Cys Pro Pro  Ser Leu Val 
    2195                 2200                 2205             


Tyr Asn  His Cys Glu His Gly  Cys Pro Arg His Cys  Asp Gly Asn 
    2210                 2215                 2220             


Val Ser  Ser Cys Gly Asp His  Pro Ser Glu Gly Cys  Phe Cys Pro 
    2225                 2230                 2235             


Pro Asp  Lys Val Met Leu Glu  Gly Ser Cys Val Pro  Glu Glu Ala 
    2240                 2245                 2250             


Cys Thr  Gln Cys Ile Gly Glu  Asp Gly Val Gln His  Gln Phe Leu 
    2255                 2260                 2265             


Glu Ala  Trp Val Pro Asp His  Gln Pro Cys Gln Ile  Cys Thr Cys 
    2270                 2275                 2280             


Leu Ser  Gly Arg Lys Val Asn  Cys Thr Thr Gln Pro  Cys Pro Thr 
    2285                 2290                 2295             


Ala Lys  Ala Pro Thr Cys Gly  Leu Cys Glu Val Ala  Arg Leu Arg 
    2300                 2305                 2310             


Gln Asn  Ala Asp Gln Cys Cys  Pro Glu Tyr Glu Cys  Val Cys Asp 
    2315                 2320                 2325             


Pro Val  Ser Cys Asp Leu Pro  Pro Val Pro His Cys  Glu Arg Gly 
    2330                 2335                 2340             


Leu Gln  Pro Thr Leu Thr Asn  Pro Gly Glu Cys Arg  Pro Asn Phe 
    2345                 2350                 2355             


Thr Cys  Ala Cys Arg Lys Glu  Glu Cys Lys Arg Val  Ser Pro Pro 
    2360                 2365                 2370             


Ser Cys  Pro Pro His Arg Leu  Pro Thr Leu Arg Lys  Thr Gln Cys 
    2375                 2380                 2385             


Cys Asp  Glu Tyr Glu Cys Ala  Cys Asn Cys Val Asn  Ser Thr Val 
    2390                 2395                 2400             


Ser Cys  Pro Leu Gly Tyr Leu  Ala Ser Thr Ala Thr  Asn Asp Cys 
    2405                 2410                 2415             


Gly Cys  Thr Thr Thr Thr Cys  Leu Pro Asp Lys Val  Cys Val His 
    2420                 2425                 2430             


Arg Ser  Thr Ile Tyr Pro Val  Gly Gln Phe Trp Glu  Glu Gly Cys 
    2435                 2440                 2445             


Asp Val  Cys Thr Cys Thr Asp  Met Glu Asp Ala Val  Met Gly Leu 
    2450                 2455                 2460             


Arg Val  Ala Gln Cys Ser Gln  Lys Pro Cys Glu Asp  Ser Cys Arg 
    2465                 2470                 2475             


Ser Gly  Phe Thr Tyr Val Leu  His Glu Gly Glu Cys  Cys Gly Arg 
    2480                 2485                 2490             


Cys Leu  Pro Ser Ala Cys Glu  Val Val Thr Gly Ser  Pro Arg Gly 
    2495                 2500                 2505             


Asp Ser  Gln Ser Ser Trp Lys  Ser Val Gly Ser Gln  Trp Ala Ser 
    2510                 2515                 2520             


Pro Glu  Asn Pro Cys Leu Ile  Asn Glu Cys Val Arg  Val Lys Glu 
    2525                 2530                 2535             


Glu Val  Phe Ile Gln Gln Arg  Asn Val Ser Cys Pro  Gln Leu Glu 
    2540                 2545                 2550             


Val Pro  Val Cys Pro Ser Gly  Phe Gln Leu Ser Cys  Lys Thr Ser 
    2555                 2560                 2565             


Ala Cys  Cys Pro Ser Cys Arg  Cys Glu Arg Met Glu  Ala Cys Met 
    2570                 2575                 2580             


Leu Asn  Gly Thr Val Ile Gly  Pro Gly Lys Thr Val  Met Ile Asp 
    2585                 2590                 2595             


Val Cys  Thr Thr Cys Arg Cys  Met Val Gln Val Gly  Val Ile Ser 
    2600                 2605                 2610             


Gly Phe  Lys Leu Glu Cys Arg  Lys Thr Thr Cys Asn  Pro Cys Pro 
    2615                 2620                 2625             


Leu Gly  Tyr Lys Glu Glu Asn  Asn Thr Gly Glu Cys  Cys Gly Arg 
    2630                 2635                 2640             


Cys Leu  Pro Thr Ala Cys Thr  Ile Gln Leu Arg Gly  Gly Gln Ile 
    2645                 2650                 2655             


Met Thr  Leu Lys Arg Asp Glu  Thr Leu Gln Asp Gly  Cys Asp Thr 
    2660                 2665                 2670             


His Phe  Cys Lys Val Asn Glu  Arg Gly Glu Tyr Phe  Trp Glu Lys 
    2675                 2680                 2685             


Arg Val  Thr Gly Cys Pro Pro  Phe Asp Glu His Lys  Cys Leu Ala 
    2690                 2695                 2700             


Glu Gly  Gly Lys Ile Met Lys  Ile Pro Gly Thr Cys  Cys Asp Thr 
    2705                 2710                 2715             


Cys Glu  Glu Pro Glu Cys Asn  Asp Ile Thr Ala Arg  Leu Gln Tyr 
    2720                 2725                 2730             


Val Lys  Val Gly Ser Cys Lys  Ser Glu Val Glu Val  Asp Ile His 
    2735                 2740                 2745             


Tyr Cys  Gln Gly Lys Cys Ala  Ser Lys Ala Met Tyr  Ser Ile Asp 
    2750                 2755                 2760             


Ile Asn  Asp Val Gln Asp Gln  Cys Ser Cys Cys Ser  Pro Thr Arg 
    2765                 2770                 2775             


Thr Glu  Pro Met Gln Val Ala  Leu His Cys Thr Asn  Gly Ser Val 
    2780                 2785                 2790             


Val Tyr  His Glu Val Leu Asn  Ala Met Glu Cys Lys  Cys Ser Pro 
    2795                 2800                 2805             


Arg Lys  Cys Ser Lys 
    2810             


