                         SEQUENCE LISTING

<110>  THE UAB RESEARCH FOUNDATION
 
<120>  Drug Screening for FGF23-FGFR4 Inhibitors

<130>  03258.0077

<150>  US 62/724,895
<151>  2019-08-30

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  802
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Arg Leu Leu Leu Ala Leu Leu Gly Val Leu Leu Ser Val Pro Gly 
1               5                   10                  15      


Pro Pro Val Leu Ser Leu Glu Ala Ser Glu Glu Val Glu Leu Glu Pro 
            20                  25                  30          


Cys Leu Ala Pro Ser Leu Glu Gln Gln Glu Gln Glu Leu Thr Val Ala 
        35                  40                  45              


Leu Gly Gln Pro Val Arg Leu Cys Cys Gly Arg Ala Glu Arg Gly Gly 
    50                  55                  60                  


His Trp Tyr Lys Glu Gly Ser Arg Leu Ala Pro Ala Gly Arg Val Arg 
65                  70                  75                  80  


Gly Trp Arg Gly Arg Leu Glu Ile Ala Ser Phe Leu Pro Glu Asp Ala 
                85                  90                  95      


Gly Arg Tyr Leu Cys Leu Ala Arg Gly Ser Met Ile Val Leu Gln Asn 
            100                 105                 110         


Leu Thr Leu Ile Thr Gly Asp Ser Leu Thr Ser Ser Asn Asp Asp Glu 
        115                 120                 125             


Asp Pro Lys Ser His Arg Asp Pro Ser Asn Arg His Ser Tyr Pro Gln 
    130                 135                 140                 


Gln Ala Pro Tyr Trp Thr His Pro Gln Arg Met Glu Lys Lys Leu His 
145                 150                 155                 160 


Ala Val Pro Ala Gly Asn Thr Val Lys Phe Arg Cys Pro Ala Ala Gly 
                165                 170                 175     


Asn Pro Thr Pro Thr Ile Arg Trp Leu Lys Asp Gly Gln Ala Phe His 
            180                 185                 190         


Gly Glu Asn Arg Ile Gly Gly Ile Arg Leu Arg His Gln His Trp Ser 
        195                 200                 205             


Leu Val Met Glu Ser Val Val Pro Ser Asp Arg Gly Thr Tyr Thr Cys 
    210                 215                 220                 


Leu Val Glu Asn Ala Val Gly Ser Ile Arg Tyr Asn Tyr Leu Leu Asp 
225                 230                 235                 240 


Val Leu Glu Arg Ser Pro His Arg Pro Ile Leu Gln Ala Gly Leu Pro 
                245                 250                 255     


Ala Asn Thr Thr Ala Val Val Gly Ser Asp Val Glu Leu Leu Cys Lys 
            260                 265                 270         


Val Tyr Ser Asp Ala Gln Pro His Ile Gln Trp Leu Lys His Ile Val 
        275                 280                 285             


Ile Asn Gly Ser Ser Phe Gly Ala Asp Gly Phe Pro Tyr Val Gln Val 
    290                 295                 300                 


Leu Lys Thr Ala Asp Ile Asn Ser Ser Glu Val Glu Val Leu Tyr Leu 
305                 310                 315                 320 


Arg Asn Val Ser Ala Glu Asp Ala Gly Glu Tyr Thr Cys Leu Ala Gly 
                325                 330                 335     


Asn Ser Ile Gly Leu Ser Tyr Gln Ser Ala Trp Leu Thr Val Leu Pro 
            340                 345                 350         


Glu Glu Asp Pro Thr Trp Thr Ala Ala Ala Pro Glu Ala Arg Tyr Thr 
        355                 360                 365             


Asp Ile Ile Leu Tyr Ala Ser Gly Ser Leu Ala Leu Ala Val Leu Leu 
    370                 375                 380                 


Leu Leu Ala Gly Leu Tyr Arg Gly Gln Ala Leu His Gly Arg His Pro 
385                 390                 395                 400 


Arg Pro Pro Ala Thr Val Gln Lys Leu Ser Arg Phe Pro Leu Ala Arg 
                405                 410                 415     


Gln Phe Ser Leu Glu Ser Gly Ser Ser Gly Lys Ser Ser Ser Ser Leu 
            420                 425                 430         


Val Arg Gly Val Arg Leu Ser Ser Ser Gly Pro Ala Leu Leu Ala Gly 
        435                 440                 445             


Leu Val Ser Leu Asp Leu Pro Leu Asp Pro Leu Trp Glu Phe Pro Arg 
    450                 455                 460                 


Asp Arg Leu Val Leu Gly Lys Pro Leu Gly Glu Gly Cys Phe Gly Gln 
465                 470                 475                 480 


Val Val Arg Ala Glu Ala Phe Gly Met Asp Pro Ala Arg Pro Asp Gln 
                485                 490                 495     


Ala Ser Thr Val Ala Val Lys Met Leu Lys Asp Asn Ala Ser Asp Lys 
            500                 505                 510         


Asp Leu Ala Asp Leu Val Ser Glu Met Glu Val Met Lys Leu Ile Gly 
        515                 520                 525             


Arg His Lys Asn Ile Ile Asn Leu Leu Gly Val Cys Thr Gln Glu Gly 
    530                 535                 540                 


Pro Leu Tyr Val Ile Val Glu Cys Ala Ala Lys Gly Asn Leu Arg Glu 
545                 550                 555                 560 


Phe Leu Arg Ala Arg Arg Pro Pro Gly Pro Asp Leu Ser Pro Asp Gly 
                565                 570                 575     


Pro Arg Ser Ser Glu Gly Pro Leu Ser Phe Pro Val Leu Val Ser Cys 
            580                 585                 590         


Ala Tyr Gln Val Ala Arg Gly Met Gln Tyr Leu Glu Ser Arg Lys Cys 
        595                 600                 605             


Ile His Arg Asp Leu Ala Ala Arg Asn Val Leu Val Thr Glu Asp Asn 
    610                 615                 620                 


Val Met Lys Ile Ala Asp Phe Gly Leu Ala Arg Gly Val His His Ile 
625                 630                 635                 640 


Asp Tyr Tyr Lys Lys Thr Ser Asn Gly Arg Leu Pro Val Lys Trp Met 
                645                 650                 655     


Ala Pro Glu Ala Leu Phe Asp Arg Val Tyr Thr His Gln Ser Asp Val 
            660                 665                 670         


Trp Ser Phe Gly Ile Leu Leu Trp Glu Ile Phe Thr Leu Gly Gly Ser 
        675                 680                 685             


Pro Tyr Pro Gly Ile Pro Val Glu Glu Leu Phe Ser Leu Leu Arg Glu 
    690                 695                 700                 


Gly His Arg Met Asp Arg Pro Pro His Cys Pro Pro Glu Leu Tyr Gly 
705                 710                 715                 720 


Leu Met Arg Glu Cys Trp His Ala Ala Pro Ser Gln Arg Pro Thr Phe 
                725                 730                 735     


Lys Gln Leu Val Glu Ala Leu Asp Lys Val Leu Leu Ala Val Ser Glu 
            740                 745                 750         


Glu Tyr Leu Asp Leu Arg Leu Thr Phe Gly Pro Tyr Ser Pro Ser Gly 
        755                 760                 765             


Gly Asp Ala Ser Ser Thr Cys Ser Ser Ser Asp Ser Val Phe Ser His 
    770                 775                 780                 


Asp Pro Leu Pro Leu Gly Ser Ser Ser Phe Pro Phe Gly Ser Gly Val 
785                 790                 795                 800 


Gln Thr 
        


<210>  2
<211>  799
<212>  PRT
<213>  Mus musculus

<400>  2

Met Trp Leu Leu Leu Ala Leu Leu Ser Ile Phe Gln Gly Thr Pro Ala 
1               5                   10                  15      


Leu Ser Leu Glu Ala Ser Glu Glu Met Glu Gln Glu Pro Cys Leu Ala 
            20                  25                  30          


Pro Ile Leu Glu Gln Gln Glu Gln Val Leu Thr Val Ala Leu Gly Gln 
        35                  40                  45              


Pro Val Arg Leu Cys Cys Gly Arg Thr Glu Arg Gly Arg His Trp Tyr 
    50                  55                  60                  


Lys Glu Gly Ser Arg Leu Ala Ser Ala Gly Arg Val Arg Gly Trp Arg 
65                  70                  75                  80  


Gly Arg Leu Glu Ile Ala Ser Phe Leu Pro Glu Asp Ala Gly Arg Tyr 
                85                  90                  95      


Leu Cys Leu Ala Arg Gly Ser Met Thr Val Val His Asn Leu Thr Leu 
            100                 105                 110         


Leu Met Asp Asp Ser Leu Thr Ser Ile Ser Asn Asp Glu Asp Pro Lys 
        115                 120                 125             


Thr Leu Ser Ser Ser Ser Ser Gly His Val Tyr Pro Gln Gln Ala Pro 
    130                 135                 140                 


Tyr Trp Thr His Pro Gln Arg Met Glu Lys Lys Leu His Ala Val Pro 
145                 150                 155                 160 


Ala Gly Asn Thr Val Lys Phe Arg Cys Pro Ala Ala Gly Asn Pro Met 
                165                 170                 175     


Pro Thr Ile His Trp Leu Lys Asp Gly Gln Ala Phe His Gly Glu Asn 
            180                 185                 190         


Arg Ile Gly Gly Ile Arg Leu Arg His Gln His Trp Ser Leu Val Met 
        195                 200                 205             


Glu Ser Val Val Pro Ser Asp Arg Gly Thr Tyr Thr Cys Leu Val Glu 
    210                 215                 220                 


Asn Ser Leu Gly Ser Ile Arg Tyr Ser Tyr Leu Leu Asp Val Leu Glu 
225                 230                 235                 240 


Arg Ser Pro His Arg Pro Ile Leu Gln Ala Gly Leu Pro Ala Asn Thr 
                245                 250                 255     


Thr Ala Val Val Gly Ser Asp Val Glu Leu Leu Cys Lys Val Tyr Ser 
            260                 265                 270         


Asp Ala Gln Pro His Ile Gln Trp Leu Lys His Val Val Ile Asn Gly 
        275                 280                 285             


Ser Ser Phe Gly Ala Asp Gly Phe Pro Tyr Val Gln Val Leu Lys Thr 
    290                 295                 300                 


Thr Asp Ile Asn Ser Ser Glu Val Glu Val Leu Tyr Leu Arg Asn Val 
305                 310                 315                 320 


Ser Ala Glu Asp Ala Gly Glu Tyr Thr Cys Leu Ala Gly Asn Ser Ile 
                325                 330                 335     


Gly Leu Ser Tyr Gln Ser Ala Trp Leu Thr Val Leu Pro Glu Glu Asp 
            340                 345                 350         


Leu Thr Trp Thr Thr Ala Thr Pro Glu Ala Arg Tyr Thr Asp Ile Ile 
        355                 360                 365             


Leu Tyr Val Ser Gly Ser Leu Val Leu Leu Val Leu Leu Leu Leu Ala 
    370                 375                 380                 


Gly Val Tyr His Arg Gln Val Ile Arg Gly His Tyr Ser Arg Gln Pro 
385                 390                 395                 400 


Val Thr Ile Gln Lys Leu Ser Arg Phe Pro Leu Ala Arg Gln Phe Ser 
                405                 410                 415     


Leu Glu Ser Arg Ser Ser Gly Lys Ser Ser Leu Ser Leu Val Arg Gly 
            420                 425                 430         


Val Arg Leu Ser Ser Ser Gly Pro Pro Leu Leu Thr Gly Leu Val Asn 
        435                 440                 445             


Leu Asp Leu Pro Leu Asp Pro Leu Trp Glu Phe Pro Arg Asp Arg Leu 
    450                 455                 460                 


Val Leu Gly Lys Pro Leu Gly Glu Gly Cys Phe Gly Gln Val Val Arg 
465                 470                 475                 480 


Ala Glu Ala Phe Gly Met Asp Pro Ser Arg Pro Asp Gln Thr Ser Thr 
                485                 490                 495     


Val Ala Val Lys Met Leu Lys Asp Asn Ala Ser Asp Lys Asp Leu Ala 
            500                 505                 510         


Asp Leu Val Ser Glu Met Glu Val Met Lys Leu Ile Gly Arg His Lys 
        515                 520                 525             


Asn Ile Ile Asn Leu Leu Gly Val Cys Thr Gln Glu Gly Pro Leu Tyr 
    530                 535                 540                 


Val Ile Val Glu Cys Ala Ala Lys Gly Asn Leu Arg Glu Phe Leu Arg 
545                 550                 555                 560 


Ala Arg Arg Pro Pro Gly Pro Asp Leu Ser Pro Asp Gly Pro Arg Ser 
                565                 570                 575     


Ser Glu Gly Pro Leu Ser Phe Pro Ala Leu Val Ser Cys Ala Tyr Gln 
            580                 585                 590         


Val Ala Arg Gly Met Gln Tyr Leu Glu Ser Arg Lys Cys Ile His Arg 
        595                 600                 605             


Asp Leu Ala Ala Arg Asn Val Leu Val Thr Glu Asp Asp Val Met Lys 
    610                 615                 620                 


Ile Ala Asp Phe Gly Leu Ala Arg Gly Val His His Ile Asp Tyr Tyr 
625                 630                 635                 640 


Lys Lys Thr Ser Asn Gly Arg Leu Pro Val Lys Trp Met Ala Pro Glu 
                645                 650                 655     


Ala Leu Phe Asp Arg Val Tyr Thr His Gln Ser Asp Val Trp Ser Phe 
            660                 665                 670         


Glu Ile Leu Leu Trp Glu Ile Phe Thr Leu Gly Gly Ser Pro Tyr Pro 
        675                 680                 685             


Gly Ile Pro Val Glu Glu Leu Phe Ser Leu Leu Arg Glu Gly His Arg 
    690                 695                 700                 


Met Glu Arg Pro Pro Asn Cys Pro Ser Glu Leu Tyr Gly Leu Met Arg 
705                 710                 715                 720 


Glu Cys Trp His Ala Ala Pro Ser Gln Arg Pro Thr Phe Lys Gln Leu 
                725                 730                 735     


Val Glu Ala Leu Asp Lys Val Leu Leu Ala Val Ser Glu Glu Tyr Leu 
            740                 745                 750         


Asp Leu Arg Leu Thr Phe Gly Pro Phe Ser Pro Ser Asn Gly Asp Ala 
        755                 760                 765             


Ser Ser Thr Cys Ser Ser Ser Asp Ser Val Phe Ser His Asp Pro Leu 
    770                 775                 780                 


Pro Leu Glu Pro Ser Pro Phe Pro Phe Ser Asp Ser Gln Thr Thr 
785                 790                 795                 


<210>  3
<211>  251
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Leu Gly Ala Arg Leu Arg Leu Trp Val Cys Ala Leu Cys Ser Val 
1               5                   10                  15      


Cys Ser Met Ser Val Leu Arg Ala Tyr Pro Asn Ala Ser Pro Leu Leu 
            20                  25                  30          


Gly Ser Ser Trp Gly Gly Leu Ile His Leu Tyr Thr Ala Thr Ala Arg 
        35                  40                  45              


Asn Ser Tyr His Leu Gln Ile His Lys Asn Gly His Val Asp Gly Ala 
    50                  55                  60                  


Pro His Gln Thr Ile Tyr Ser Ala Leu Met Ile Arg Ser Glu Asp Ala 
65                  70                  75                  80  


Gly Phe Val Val Ile Thr Gly Val Met Ser Arg Arg Tyr Leu Cys Met 
                85                  90                  95      


Asp Phe Arg Gly Asn Ile Phe Gly Ser His Tyr Phe Asp Pro Glu Asn 
            100                 105                 110         


Cys Arg Phe Gln His Gln Thr Leu Glu Asn Gly Tyr Asp Val Tyr His 
        115                 120                 125             


Ser Pro Gln Tyr His Phe Leu Val Ser Leu Gly Arg Ala Lys Arg Ala 
    130                 135                 140                 


Phe Leu Pro Gly Met Asn Pro Pro Pro Tyr Ser Gln Phe Leu Ser Arg 
145                 150                 155                 160 


Arg Asn Glu Ile Pro Leu Ile His Phe Asn Thr Pro Ile Pro Arg Arg 
                165                 170                 175     


His Thr Arg Ser Ala Glu Asp Asp Ser Glu Arg Asp Pro Leu Asn Val 
            180                 185                 190         


Leu Lys Pro Arg Ala Arg Met Thr Pro Ala Pro Ala Ser Cys Ser Gln 
        195                 200                 205             


Glu Leu Pro Ser Ala Glu Asp Asn Ser Pro Met Ala Ser Asp Pro Leu 
    210                 215                 220                 


Gly Val Val Arg Gly Gly Arg Val Asn Thr His Ala Gly Gly Thr Gly 
225                 230                 235                 240 


Pro Glu Gly Cys Arg Pro Phe Ala Lys Phe Ile 
                245                 250     


<210>  4
<211>  251
<212>  PRT
<213>  Mus musculus

<400>  4

Met Leu Gly Thr Cys Leu Arg Leu Leu Val Gly Val Leu Cys Thr Val 
1               5                   10                  15      


Cys Ser Leu Gly Thr Ala Arg Ala Tyr Pro Asp Thr Ser Pro Leu Leu 
            20                  25                  30          


Gly Ser Asn Trp Gly Ser Leu Thr His Leu Tyr Thr Ala Thr Ala Arg 
        35                  40                  45              


Thr Ser Tyr His Leu Gln Ile His Arg Asp Gly His Val Asp Gly Thr 
    50                  55                  60                  


Pro His Gln Thr Ile Tyr Ser Ala Leu Met Ile Thr Ser Glu Asp Ala 
65                  70                  75                  80  


Gly Ser Val Val Ile Thr Gly Ala Met Thr Arg Arg Phe Leu Cys Met 
                85                  90                  95      


Asp Leu His Gly Asn Ile Phe Gly Ser Leu His Phe Ser Pro Glu Asn 
            100                 105                 110         


Cys Lys Phe Arg Gln Trp Thr Leu Glu Asn Gly Tyr Asp Val Tyr Leu 
        115                 120                 125             


Ser Gln Lys His His Tyr Leu Val Ser Leu Gly Arg Ala Lys Arg Ile 
    130                 135                 140                 


Phe Gln Pro Gly Thr Asn Pro Pro Pro Phe Ser Gln Phe Leu Ala Arg 
145                 150                 155                 160 


Arg Asn Glu Val Pro Leu Leu His Phe Tyr Thr Val Arg Pro Arg Arg 
                165                 170                 175     


His Thr Arg Ser Ala Glu Asp Pro Pro Glu Arg Asp Pro Leu Asn Val 
            180                 185                 190         


Leu Lys Pro Arg Pro Arg Ala Thr Pro Val Pro Val Ser Cys Ser Arg 
        195                 200                 205             


Glu Leu Pro Ser Ala Glu Glu Gly Gly Pro Ala Ala Ser Asp Pro Leu 
    210                 215                 220                 


Gly Val Leu Arg Arg Gly Arg Gly Asp Ala Arg Gly Gly Ala Gly Gly 
225                 230                 235                 240 


Ala Asp Arg Cys Arg Pro Phe Pro Arg Phe Val 
                245                 250     


<210>  5
<211>  585
<212>  PRT
<213>  Homo sapiens

<400>  5

Leu Glu Ala Ser Glu Glu Val Glu Leu Glu Pro Cys Leu Ala Pro Ser 
1               5                   10                  15      


Leu Glu Gln Gln Glu Gln Glu Leu Thr Val Ala Leu Gly Gln Pro Val 
            20                  25                  30          


Arg Leu Cys Cys Gly Arg Ala Glu Arg Gly Gly His Trp Tyr Lys Glu 
        35                  40                  45              


Gly Ser Arg Leu Ala Pro Ala Gly Arg Val Arg Gly Trp Arg Gly Arg 
    50                  55                  60                  


Leu Glu Ile Ala Ser Phe Leu Pro Glu Asp Ala Gly Arg Tyr Leu Cys 
65                  70                  75                  80  


Leu Ala Arg Gly Ser Met Ile Val Leu Gln Asn Leu Thr Leu Ile Thr 
                85                  90                  95      


Gly Asp Ser Leu Thr Ser Ser Asn Asp Asp Glu Asp Pro Lys Ser His 
            100                 105                 110         


Arg Asp Pro Ser Asn Arg His Ser Tyr Pro Gln Gln Ala Pro Tyr Trp 
        115                 120                 125             


Thr His Pro Gln Arg Met Glu Lys Lys Leu His Ala Val Pro Ala Gly 
    130                 135                 140                 


Asn Thr Val Lys Phe Arg Cys Pro Ala Ala Gly Asn Pro Thr Pro Thr 
145                 150                 155                 160 


Ile Arg Trp Leu Lys Asp Gly Gln Ala Phe His Gly Glu Asn Arg Ile 
                165                 170                 175     


Gly Gly Ile Arg Leu Arg His Gln His Trp Ser Leu Val Met Glu Ser 
            180                 185                 190         


Val Val Pro Ser Asp Arg Gly Thr Tyr Thr Cys Leu Val Glu Asn Ala 
        195                 200                 205             


Val Gly Ser Ile Arg Tyr Asn Tyr Leu Leu Asp Val Leu Glu Arg Ser 
    210                 215                 220                 


Pro His Arg Pro Ile Leu Gln Ala Gly Leu Pro Ala Asn Thr Thr Ala 
225                 230                 235                 240 


Val Val Gly Ser Asp Val Glu Leu Leu Cys Lys Val Tyr Ser Asp Ala 
                245                 250                 255     


Gln Pro His Ile Gln Trp Leu Lys His Ile Val Ile Asn Gly Ser Ser 
            260                 265                 270         


Phe Gly Ala Asp Gly Phe Pro Tyr Val Gln Val Leu Lys Thr Ala Asp 
        275                 280                 285             


Ile Asn Ser Ser Glu Val Glu Val Leu Tyr Leu Arg Asn Val Ser Ala 
    290                 295                 300                 


Glu Asp Ala Gly Glu Tyr Thr Cys Leu Ala Gly Asn Ser Ile Gly Leu 
305                 310                 315                 320 


Ser Tyr Gln Ser Ala Trp Leu Thr Val Leu Pro Glu Glu Asp Pro Thr 
                325                 330                 335     


Trp Thr Ala Ala Ala Pro Glu Ala Arg Tyr Thr Asp Glu Ile Gly Arg 
            340                 345                 350         


Met Asp Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        355                 360                 365             


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
    370                 375                 380                 


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
385                 390                 395                 400 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
                405                 410                 415     


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
            420                 425                 430         


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
        435                 440                 445             


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
    450                 455                 460                 


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
465                 470                 475                 480 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
                485                 490                 495     


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
            500                 505                 510         


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
        515                 520                 525             


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
    530                 535                 540                 


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
545                 550                 555                 560 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
                565                 570                 575     


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            580                 585 


