                               SEQUENCE LISTING

<110> GENOMATICA, INC.
 
<120> XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED 
      CO-UTILIZATION OF GLUCOSE AND XYLOSE

<130> 109112-0912

<140> PCT/US2019/048888
<141> 2019-08-29

<150> 62/731,711
<151> 2018-09-14

<150> 62/726,114
<151> 2018-08-31

<160> 5     

<170> PatentIn version 3.5

<210> 1
<211> 392
<212> PRT
<213> Escherichia coli

<400> 1
Met Phe Thr Lys Arg His Arg Ile Thr Leu Leu Phe Asn Ala Asn Lys 
1               5                   10                  15      


Ala Tyr Asp Arg Gln Val Val Glu Gly Val Gly Glu Tyr Leu Gln Ala 
            20                  25                  30          


Ser Gln Ser Glu Trp Asp Ile Phe Ile Glu Glu Asp Phe Arg Ala Arg 
        35                  40                  45              


Ile Asp Lys Ile Lys Asp Trp Leu Gly Asp Gly Val Ile Ala Asp Phe 
    50                  55                  60                  


Asp Asp Lys Gln Ile Glu Gln Ala Leu Ala Asp Val Asp Val Pro Ile 
65                  70                  75                  80  


Val Gly Val Gly Gly Ser Tyr His Leu Ala Glu Ser Tyr Pro Pro Val 
                85                  90                  95      


His Tyr Ile Ala Thr Asp Asn Tyr Ala Leu Val Glu Ser Ala Phe Leu 
            100                 105                 110         


His Leu Lys Glu Lys Gly Val Asn Arg Phe Ala Phe Tyr Gly Leu Pro 
        115                 120                 125             


Glu Ser Ser Gly Lys Arg Trp Ala Thr Glu Arg Glu Tyr Ala Phe Arg 
    130                 135                 140                 


Gln Leu Val Ala Glu Glu Lys Tyr Arg Gly Val Val Tyr Gln Gly Leu 
145                 150                 155                 160 


Glu Thr Ala Pro Glu Asn Trp Gln His Ala Gln Asn Arg Leu Ala Asp 
                165                 170                 175     


Trp Leu Gln Thr Leu Pro Pro Gln Thr Gly Ile Ile Ala Val Thr Asp 
            180                 185                 190         


Ala Arg Ala Arg His Ile Leu Gln Val Cys Glu His Leu His Ile Pro 
        195                 200                 205             


Val Pro Glu Lys Leu Cys Val Ile Gly Ile Asp Asn Glu Glu Leu Thr 
    210                 215                 220                 


Arg Tyr Leu Ser Arg Val Ala Leu Ser Ser Val Ala Gln Gly Ala Arg 
225                 230                 235                 240 


Gln Met Gly Tyr Gln Ala Ala Lys Leu Leu His Arg Leu Leu Asp Lys 
                245                 250                 255     


Glu Glu Met Pro Leu Gln Arg Ile Leu Val Pro Pro Val Arg Val Ile 
            260                 265                 270         


Glu Arg Arg Ser Thr Asp Tyr Arg Ser Leu Thr Asp Pro Ala Val Ile 
        275                 280                 285             


Gln Ala Met His Tyr Ile Arg Asn His Ala Cys Lys Gly Ile Lys Val 
    290                 295                 300                 


Asp Gln Val Leu Asp Ala Val Gly Ile Ser Arg Ser Asn Leu Glu Lys 
305                 310                 315                 320 


Arg Phe Lys Glu Glu Val Gly Glu Thr Ile His Ala Met Ile His Ala 
                325                 330                 335     


Glu Lys Leu Glu Lys Ala Arg Ser Leu Leu Ile Ser Thr Thr Leu Ser 
            340                 345                 350         


Ile Asn Glu Ile Ser Gln Met Cys Gly Tyr Pro Ser Leu Gln Tyr Phe 
        355                 360                 365             


Tyr Ser Val Phe Lys Lys Ala Tyr Asp Thr Thr Pro Lys Glu Tyr Arg 
    370                 375                 380                 


Asp Val Asn Ser Glu Val Met Leu 
385                 390         


<210> 2
<211> 1179
<212> DNA
<213> Escherichia coli

<400> 2
atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc ctatgaccgg       60

caggtagtag aaggcgtagg ggaatattta caggcgtcac aatcggaatg ggatattttc      120

attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg agatggcgtc      180

attgccgact tcgacgacaa acagatcgag caagcgctgg ctgatgtcga cgtccccatt      240

gttggggttg gcggctcgta tcaccttgca gaaagttacc cacccgttca ttacattgcc      300

accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa aggcgttaac      360

cgctttgctt tttatggtct tccggaatca agcggcaaac gttgggccac tgagcgcgaa      420

tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta tcaggggtta      480

gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc tggcagactg gctacaaacg      540

ctaccaccgc aaaccgggat tattgccgtt actgacgccc gagcgcggca tattctgcaa      600

gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg catcgataac      660

gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca gggcgcgcgg      720

caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga agaaatgccg      780

ctacagcgaa ttttggtccc accagttcgc gtcattgaac ggcgctcaac agattatcgc      840

tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca cgcctgtaaa      900

gggattaaag tggatcaggt actggatgcg gtcgggatct cgcgctccaa tcttgagaag      960

cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga gaagctggag     1020

aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc gcaaatgtgc     1080

ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga cacgacgcca     1140

aaagagtatc gcgatgtaaa tagcgaggtc atgttgtag                            1179


<210> 3
<211> 392
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Met Phe Thr Lys Arg His Arg Ile Thr Leu Leu Phe Asn Ala Asn Lys 
1               5                   10                  15      


Ala Tyr Asp Arg Gln Val Val Glu Gly Val Gly Glu Tyr Leu Gln Ala 
            20                  25                  30          


Ser Gln Ser Glu Trp Asp Ile Phe Ile Glu Glu Asp Phe Arg Ala Arg 
        35                  40                  45              


Ile Asp Lys Ile Lys Asp Trp Leu Gly Asp Gly Val Ile Ala Asp Phe 
    50                  55                  60                  


Asp Asp Lys Gln Ile Glu Gln Ala Leu Ala Asp Val Asp Val Pro Ile 
65                  70                  75                  80  


Val Gly Val Gly Gly Ser Tyr His Leu Ala Glu Ser Tyr Pro Pro Val 
                85                  90                  95      


His Tyr Ile Ala Thr Asp Asn Tyr Ala Leu Val Glu Ser Ala Phe Leu 
            100                 105                 110         


His Leu Lys Glu Lys Gly Val Asn Arg Phe Ala Phe Tyr Gly Leu Pro 
        115                 120                 125             


Glu Ser Ser Gly Lys Arg Trp Ala Thr Glu Arg Glu Tyr Ala Phe Arg 
    130                 135                 140                 


Gln Leu Val Ala Glu Glu Lys Tyr Arg Gly Val Val Tyr Gln Gly Leu 
145                 150                 155                 160 


Glu Thr Ala Pro Glu Asn Trp Gln His Ala Gln Asn Arg Leu Ala Asp 
                165                 170                 175     


Trp Leu Gln Thr Leu Pro Pro Gln Thr Gly Ile Ile Ala Val Thr Asp 
            180                 185                 190         


Ala Arg Ala Arg His Ile Leu Gln Val Cys Glu His Leu His Ile Pro 
        195                 200                 205             


Val Pro Glu Lys Leu Cys Val Ile Gly Ile Asp Asn Glu Glu Leu Thr 
    210                 215                 220                 


Arg Tyr Leu Ser Arg Val Ala Leu Ser Ser Val Ala Gln Gly Ala Arg 
225                 230                 235                 240 


Gln Met Gly Tyr Gln Ala Ala Lys Leu Leu His Arg Leu Leu Asp Lys 
                245                 250                 255     


Glu Glu Met Pro Leu Gln Arg Ile Leu Val Pro Pro Val Arg Val Ile 
            260                 265                 270         


Glu Arg Arg Ser Thr Asp Tyr Arg Ser Leu Thr Asp Pro Ala Val Ile 
        275                 280                 285             


Gln Ala Met His Tyr Ile Arg Asn His Ala Cys Lys Gly Ile Lys Val 
    290                 295                 300                 


Asp Gln Val Leu Asp Ala Val Gly Ile Ser Arg Ser Asn Leu Glu Lys 
305                 310                 315                 320 


Arg Phe Lys Glu Glu Val Gly Glu Thr Ile His Ala Met Ile His Ala 
                325                 330                 335     


Glu Lys Leu Glu Lys Ala Arg Ser Leu Leu Ile Ser Thr Thr Leu Ser 
            340                 345                 350         


Ile Asn Glu Ile Ser Gln Met Cys Gly Tyr Pro Ser Leu Gln Tyr Phe 
        355                 360                 365             


Tyr Ser Val Phe Lys Lys Ala Tyr Asp Thr Thr Pro Lys Lys Tyr Arg 
    370                 375                 380                 


Asp Val Asn Ser Glu Val Met Leu 
385                 390         


<210> 4
<211> 1179
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 4
atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc ctatgaccgg       60

caggtagtag aaggcgtagg ggaatattta caggcgtcac aatcggaatg ggatattttc      120

attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg agatggcgtc      180

attgccgact tcgacgacaa acagatcgag caagcgctgg ctgatgtcga cgtccccatt      240

gttggggttg gcggctcgta tcaccttgca gaaagttacc cacccgttca ttacattgcc      300

accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa aggcgttaac      360

cgctttgctt tttatggtct tccggaatca agcggcaaac gttgggccac tgagcgcgaa      420

tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta tcaggggtta      480

gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc tggcagactg gctacaaacg      540

ctaccaccgc aaaccgggat tattgccgtt actgacgccc gagcgcggca tattctgcaa      600

gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg catcgataac      660

gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca gggcgcgcgg      720

caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga agaaatgccg      780

ctacagcgaa ttttggtccc accagttcgc gtcattgaac ggcgctcaac agattatcgc      840

tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca cgcctgtaaa      900

gggattaaag tggatcaggt actggatgcg gtcgggatct cgcgctccaa tcttgagaag      960

cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga gaagctggag     1020

aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc gcaaatgtgc     1080

ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga cacgacgcca     1140

aaaaagtatc gcgatgtaaa tagcgaggtc atgttgtag                            1179


<210> 5
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 5
His His His His His His 
1               5       

