                         SEQUENCE LISTING

<110>  Seattle Children's Hospital (dba Seattle Children's 
       Research Institute)
 
<120>  METHODS AND COMPOSITONS COMPRISING B7H3 CHIMERIC ANTIGEN 
       RECEPTORS

<130>  SCRI.170WO

<150>  US 62/725599
<151>  2018-08-31

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; VH CDR1 polypeptide

<400>  1

Phe Gly Met His 
1               


<210>  2
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; VH CDR2 polypeptide

<400>  2

Tyr Ile Ser Ser Asp Ser Ser Ala Ile Tyr Tyr Ala Asp Thr Val Lys 
1               5                   10                  15      


<210>  3
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; VH CDR3 polypeptide

<400>  3

Gly Arg Glu Asn Ile Tyr Tyr Gly Ser Arg Leu Asp Tyr 
1               5                   10              


<210>  4
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; VL CDR1 polypeptide

<400>  4

Lys Ala Ser Gln Asn Val Asp Thr Asn Val Ala 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; VL CDR2 polypeptide

<400>  5

Ser Ala Ser Tyr Arg Tyr Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; VL CDR3 polypeptide

<400>  6

Gln Gln Tyr Asn Asn Tyr Pro Phe Thr 
1               5                   


<210>  7
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; VH CDR1 nucleic acid

<400>  7
tttggaatgc ac                                                           12


<210>  8
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; VH CDR2 nucleic acid

<400>  8
tacattagta gtgacagtag tgccatctac tatgcagaca cagtgaag                    48


<210>  9
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; VH CDR3 nucleic acid

<400>  9
gggagggaaa acatttacta cggtagtagg cttgactac                              39


<210>  10
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; VL CDR1 nucleic acid

<400>  10
aaggccagtc agaatgtgga tactaatgta gcc                                    33


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; VL CDR2 nucleic acid

<400>  11
tcggcatcct accggtacag t                                                 21


<210>  12
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; VL CDR3 nucleic acid

<400>  12
cagcaatata acaactatcc attcacg                                           27


<210>  13
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; VH region polypeptide

<400>  13

Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Arg Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Glu Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Ser Ser Asp Ser Ser Ala Ile Tyr Tyr Ala Asp Thr Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Pro Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Thr Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Gly Arg Gly Arg Glu Asn Ile Tyr Tyr Gly Ser Arg Leu Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser 
        115                 120         


<210>  14
<211>  366
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; VH region nucleic acid

<400>  14
gatgtgcagc tggtggagtc tgggggaggc ttagtgcagc ctggagggtc ccggaaactc       60

tcctgtgcag cctctggatt cactttcagt agctttggaa tgcactgggt tcgtcaggct      120

ccagagaagg ggctggagtg ggtcgcatac attagtagtg acagtagtgc catctactat      180

gcagacacag tgaagggccg attcaccatc tccagagaca atcccaagaa caccctgttc      240

ctgcaaatga ccagtctaag gtctgaggac acggccatgt attactgtgg aagagggagg      300

gaaaacattt actacggtag taggcttgac tactggggcc aaggcaccac tctcacagtc      360

tcctca                                                                 366


<210>  15
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; VL region polypeptide

<400>  15

Asp Ile Ala Met Thr Gln Ser Gln Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Val Thr Cys Lys Ala Ser Gln Asn Val Asp Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Ala Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Asn Val Gln Ser 
65                  70                  75                  80  


Glu Asp Leu Ala Glu Tyr Phe Cys Gln Gln Tyr Asn Asn Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  16
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; VL region nucleic acid

<400>  16
gacattgcga tgacccagtc tcaaaaattc atgtccacat cagtaggaga cagggtcagc       60

gtcacctgca aggccagtca gaatgtggat actaatgtag cctggtatca acagaaacca      120

gggcaatctc ctaaagcact gatttactcg gcatcctacc ggtacagtgg agtccctgat      180

cgcttcacag gcagtggatc tgggacagat ttcactctca ccatcaacaa tgtgcagtct      240

gaagacttgg cagagtattt ctgtcagcaa tataacaact atccattcac gttcggctcg      300

gggacaaagt tggaaataaa a                                                321


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; spacer region


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  X = Cys, Gly, or Arg

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X = Cys, or Thr

<400>  17

Xaa Pro Pro Xaa Pro 
1               5   


<210>  18
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; Small (S) spacer

<400>  18

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  19
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; Medium (M) spacer

<400>  19

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg 
1               5                   10                  15      


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
            20                  25                  30          


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
        35                  40                  45              


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
    50                  55                  60                  


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
65                  70                  75                  80  


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
                85                  90                  95      


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
            100                 105                 110         


Leu Ser Leu Ser Leu Gly Lys 
        115                 


<210>  20
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; Long (L) spacer

<400>  20

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  21
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; Human IgG1 hinge

<400>  21

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  22
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; Human IgG2 hinge

<400>  22

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  23
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; Human IgG3 hinge

<400>  23

Glu Leu Lys Thr Pro Leu Gly Asp Thr His Thr Cys Pro Arg Cys Pro 
1               5                   10                  15      


<210>  24
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; Human IgG4 hinge

<400>  24

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
1               5                   10          


<210>  25
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; Modified human IgG4 hinge

<400>  25

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  26
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; Modified human IgG4 hinge

<400>  26

Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   


<210>  27
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; Modified human IgG4 hinge

<400>  27

Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10  


<210>  28
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; Modified human IgG4 hinge

<400>  28

Glu Val Val Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10              


<210>  29
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; CD28tm nucleotide sequence

<400>  29
atgttctggg tgctggtggt ggtcggaggc gtgctggcct gctacagcct gctggtcacc       60

gtggccttca tcatcttttg ggtg                                              84


<210>  30
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic; CD28tm amino acid sequence

<400>  30

Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
1               5                   10                  15      


Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25              


<210>  31
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; CD3-zeta signaling domain nucleotide sequence

<400>  31
cgggtgaagt tcagcagaag cgccgacgcc cctgcctacc agcagggcca gaatcagctg       60

tacaacgagc tgaacctggg cagaagggaa gagtacgacg tcctggataa gcggagaggc      120

cgggaccctg agatgggcgg caagcctcgg cggaagaacc cccaggaagg cctgtataac      180

gaactgcaga aagacaagat ggccgaggcc tacagcgaga tcggcatgaa gggcgagcgg      240

aggcggggca agggccacga cggcctgtat cagggcctgt ccaccgccac caaggatacc      300

tacgacgccc tgcacatgca ggccctgccc ccaagg                                336


<210>  32
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; 4-1BB signaling domain nucleotide sequence

<400>  32
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa       60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt      120

gaactg                                                                 126


<210>  33
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; T2A nucleotide sequence

<400>  33
ctcgagggcg gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat       60

cccggcccta gg                                                           72


<210>  34
<211>  1071
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic; EGFRt nucleotide sequence

<400>  34
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccacgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata      120

aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc      180

ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa      240

ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct      300

gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag      360

caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc      420

tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat      480

gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata      540

agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc      600

cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga      660

ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag      720

aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc      780

acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc      840

gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca      900

gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca      960

ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg     1020

ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat g              1071


