                         SEQUENCE LISTING

<110>  10x Genomics, Inc.
       Schnall-Levin, Michael
       Lucero, Michael  Ybarra
       Mikkelsen, Tarjei Sigurd
       Stahl, Patrik
       Frisen, Jonas
       Marklund, Maja
       Llorens, Enric
 
<120>  METHOD FOR TRANSPOSASE-MEDIATED SPATIAL TAGGING AND ANALYZING 
       GENOMIC DNA IN A BIOLOGICAL SAMPLE

<130>  47706-0036WO1

<140>  PCT/US2019/048425
<141>  2019-08-27

<150>  US 62/724,483
<151>  2018-08-29

<150>  US 62/779,342
<151>  2018-12-13

<150>  US 62/723,950
<151>  2018-08-28

<150>  US 62/723,957
<151>  2018-08-28

<150>  US 62/723,960
<151>  2018-08-28

<150>  US 62/723,964
<151>  2018-08-28

<150>  US 62/723,970
<151>  2018-08-28

<150>  US 62/723,972
<151>  2018-08-28

<150>  US 62/724,489
<151>  2018-08-29

<150>  US 62/724,561
<151>  2018-08-29

<150>  US 62/788,905
<151>  2019-01-06

<150>  US 62/788,867
<151>  2019-01-06

<150>  US 62/788,871
<151>  2019-01-06

<150>  US 62/788,897
<151>  2019-01-06

<150>  US 62/788,885
<151>  2019-01-06

<150>  US 62/822,565
<151>  2019-03-22

<150>  US 62/819,496
<151>  2019-03-15

<150>  US 62/819,486
<151>  2019-03-15

<150>  US 62/819,467
<151>  2019-03-15

<150>  US 62/822,632
<151>  2019-03-22

<150>  US 62/822,618
<151>  2019-03-22

<150>  US 62/822,592
<151>  2019-03-22

<150>  US 62/819,468
<151>  2019-03-15

<150>  US 62/822,627
<151>  2019-03-22

<150>  US 62/819,448
<151>  2019-03-15

<150>  US 62/822,649
<151>  2019-03-22

<150>  US 62/819,456
<151>  2019-03-15

<150>  US 62/819,478
<151>  2019-03-15

<150>  US 62/819,449
<151>  2019-03-15

<150>  US 62/822,554
<151>  2019-03-22

<150>  US 62/822,575
<151>  2019-03-22

<150>  US 62/822,605
<151>  2019-03-22

<150>  US 62/812,219
<151>  2019-02-28

<150>  US 62/819,458
<151>  2019-03-15

<150>  US 62/839,223
<151>  2019-04-26

<150>  US 62/839,320
<151>  2019-04-26

<150>  US 62/839,346
<151>  2019-04-26

<150>  US 62/842,463
<151>  2019-05-02

<150>  US 62/860,993
<151>  2019-06-13

<150>  US 62/839,526
<151>  2019-04-26

<150>  US 62/858,331
<151>  2019-06-07

<150>  US 62/724,487
<151>  2018-08-29

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  476
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Ile Thr Ser Ala Leu His Arg Ala Ala Asp Trp Ala Lys Ser Val 
1               5                   10                  15      


Phe Ser Ser Ala Ala Leu Gly Asp Pro Arg Arg Thr Ala Arg Leu Val 
            20                  25                  30          


Asn Val Ala Ala Gln Leu Ala Lys Tyr Ser Gly Lys Ser Ile Thr Ile 
        35                  40                  45              


Ser Ser Glu Gly Ser Glu Ala Met Gln Glu Gly Ala Tyr Arg Phe Ile 
    50                  55                  60                  


Arg Asn Pro Asn Val Ser Ala Glu Ala Ile Arg Lys Ala Gly Ala Met 
65                  70                  75                  80  


Gln Thr Val Lys Leu Ala Gln Glu Phe Pro Glu Leu Leu Ala Ile Glu 
                85                  90                  95      


Asp Thr Thr Ser Leu Ser Tyr Arg His Gln Val Ala Glu Glu Leu Gly 
            100                 105                 110         


Lys Leu Gly Ser Ile Gln Asp Lys Ser Arg Gly Trp Trp Val His Ser 
        115                 120                 125             


Val Leu Leu Leu Glu Ala Thr Thr Phe Arg Thr Val Gly Leu Leu His 
    130                 135                 140                 


Gln Glu Trp Trp Met Arg Pro Asp Asp Pro Ala Asp Ala Asp Glu Lys 
145                 150                 155                 160 


Glu Ser Gly Lys Trp Leu Ala Ala Ala Ala Thr Ser Arg Leu Arg Met 
                165                 170                 175     


Gly Ser Met Met Ser Asn Val Ile Ala Val Cys Asp Arg Glu Ala Asp 
            180                 185                 190         


Ile His Ala Tyr Leu Gln Asp Lys Leu Ala His Asn Glu Arg Phe Val 
        195                 200                 205             


Val Arg Ser Lys His Pro Arg Lys Asp Val Glu Ser Gly Leu Tyr Leu 
    210                 215                 220                 


Tyr Asp His Leu Lys Asn Gln Pro Glu Leu Gly Gly Tyr Gln Ile Ser 
225                 230                 235                 240 


Ile Pro Gln Lys Gly Val Val Asp Lys Arg Gly Lys Arg Lys Asn Arg 
                245                 250                 255     


Pro Ala Arg Lys Ala Ser Leu Ser Leu Arg Ser Gly Arg Ile Thr Leu 
            260                 265                 270         


Lys Gln Gly Asn Ile Thr Leu Asn Ala Val Leu Ala Glu Glu Ile Asn 
        275                 280                 285             


Pro Pro Lys Gly Glu Thr Pro Leu Lys Trp Leu Leu Leu Thr Ser Glu 
    290                 295                 300                 


Pro Val Glu Ser Leu Ala Gln Ala Leu Arg Val Ile Asp Ile Tyr Thr 
305                 310                 315                 320 


His Arg Trp Arg Ile Glu Glu Phe His Lys Ala Trp Lys Thr Gly Ala 
                325                 330                 335     


Gly Ala Glu Arg Gln Arg Met Glu Glu Pro Asp Asn Leu Glu Arg Met 
            340                 345                 350         


Val Ser Ile Leu Ser Phe Val Ala Val Arg Leu Leu Gln Leu Arg Glu 
        355                 360                 365             


Ser Phe Thr Leu Pro Gln Ala Leu Arg Ala Gln Gly Leu Leu Lys Glu 
    370                 375                 380                 


Ala Glu His Val Glu Ser Gln Ser Ala Glu Thr Val Leu Thr Pro Asp 
385                 390                 395                 400 


Glu Cys Gln Leu Leu Gly Tyr Leu Asp Lys Gly Lys Arg Lys Arg Lys 
                405                 410                 415     


Glu Lys Ala Gly Ser Leu Gln Trp Ala Tyr Met Ala Ile Ala Arg Leu 
            420                 425                 430         


Gly Gly Phe Met Asp Ser Lys Arg Thr Gly Ile Ala Ser Trp Gly Ala 
        435                 440                 445             


Leu Trp Glu Gly Trp Glu Ala Leu Gln Ser Lys Leu Asp Gly Phe Leu 
    450                 455                 460                 


Ala Ala Lys Asp Leu Met Ala Gln Gly Ile Lys Ile 
465                 470                 475     


<210>  2
<211>  662
<212>  PRT
<213>  Bacteriophage Mu

<400>  2

Met Lys Glu Trp Tyr Thr Ala Lys Glu Leu Leu Gly Leu Ala Gly Leu 
1               5                   10                  15      


Pro Lys Gln Ala Thr Asn Ile Thr Arg Lys Ala Gln Arg Glu Gly Trp 
            20                  25                  30          


Glu Phe Arg Gln Val Ala Gly Thr Lys Gly Val Ser Phe Glu Phe Asn 
        35                  40                  45              


Ile Lys Ser Phe Pro Val Ala Leu Arg Ala Glu Ile Leu Leu Gln Gln 
    50                  55                  60                  


Gly Arg Ile Glu Thr Ser Gln Gly Tyr Phe Glu Ile Ala Arg Pro Thr 
65                  70                  75                  80  


Leu Glu Ala His Asp Tyr Asp Arg Glu Ala Leu Trp Ser Lys Trp Asp 
                85                  90                  95      


Asn Ala Ser Asp Ser Gln Arg Arg Leu Ala Glu Lys Trp Leu Pro Ala 
            100                 105                 110         


Val Gln Ala Ala Asp Glu Met Leu Asn Gln Gly Ile Ser Thr Lys Thr 
        115                 120                 125             


Ala Phe Ala Thr Val Ala Gly His Tyr Gln Val Ser Ala Ser Thr Leu 
    130                 135                 140                 


Arg Asp Lys Tyr Tyr Gln Val Gln Lys Phe Ala Lys Pro Asp Trp Ala 
145                 150                 155                 160 


Ala Ala Leu Val Asp Gly Arg Gly Ala Ser Arg Arg Asn Val His Lys 
                165                 170                 175     


Ser Glu Phe Asp Glu Asp Ala Trp Gln Phe Leu Ile Ala Asp Tyr Leu 
            180                 185                 190         


Arg Pro Glu Lys Pro Ala Phe Arg Lys Cys Tyr Glu Arg Leu Glu Leu 
        195                 200                 205             


Ala Ala Arg Glu His Gly Trp Ser Ile Pro Ser Arg Ala Thr Ala Phe 
    210                 215                 220                 


Arg Arg Ile Gln Gln Leu Asp Glu Ala Met Val Val Ala Cys Arg Glu 
225                 230                 235                 240 


Gly Glu His Ala Leu Met His Leu Ile Pro Ala Gln Gln Arg Thr Val 
                245                 250                 255     


Glu His Leu Asp Ala Met Gln Trp Ile Asn Gly Asp Gly Tyr Leu His 
            260                 265                 270         


Asn Val Phe Val Arg Trp Phe Asn Gly Asp Val Ile Arg Pro Lys Thr 
        275                 280                 285             


Trp Phe Trp Gln Asp Val Lys Thr Arg Lys Ile Leu Gly Trp Arg Cys 
    290                 295                 300                 


Asp Val Ser Glu Asn Ile Asp Ser Ile Arg Leu Ser Phe Met Asp Val 
305                 310                 315                 320 


Val Thr Arg Tyr Gly Ile Pro Glu Asp Phe His Ile Thr Ile Asp Asn 
                325                 330                 335     


Thr Arg Gly Ala Ala Asn Lys Trp Leu Thr Gly Gly Ala Pro Asn Arg 
            340                 345                 350         


Tyr Arg Phe Lys Val Lys Glu Asp Asp Pro Lys Gly Leu Phe Leu Leu 
        355                 360                 365             


Met Gly Ala Lys Met His Trp Thr Ser Val Val Ala Gly Lys Gly Trp 
    370                 375                 380                 


Gly Gln Ala Lys Pro Val Glu Arg Ala Phe Gly Val Gly Gly Leu Glu 
385                 390                 395                 400 


Glu Tyr Val Asp Lys His Pro Ala Leu Ala Gly Ala Tyr Thr Gly Pro 
                405                 410                 415     


Asn Pro Gln Ala Lys Pro Asp Asn Tyr Gly Asp Arg Ala Val Asp Ala 
            420                 425                 430         


Glu Leu Phe Leu Lys Thr Leu Ala Glu Gly Val Ala Met Phe Asn Ala 
        435                 440                 445             


Arg Thr Gly Arg Glu Thr Glu Met Cys Gly Gly Lys Leu Ser Phe Asp 
    450                 455                 460                 


Asp Val Phe Glu Arg Glu Tyr Ala Arg Thr Ile Val Arg Lys Pro Thr 
465                 470                 475                 480 


Glu Glu Gln Lys Arg Met Leu Leu Leu Pro Ala Glu Ala Val Asn Val 
                485                 490                 495     


Ser Arg Lys Gly Glu Phe Ala Leu Lys Val Gly Gly Ser Leu Lys Gly 
            500                 505                 510         


Ala Lys Asn Val Tyr Tyr Asn Met Ala Leu Met Asn Ala Gly Val Lys 
        515                 520                 525             


Lys Val Val Val Arg Phe Asp Pro Gln Gln Leu His Ser Thr Val Tyr 
    530                 535                 540                 


Cys Tyr Thr Leu Asp Gly Arg Phe Ile Cys Glu Ala Glu Cys Leu Ala 
545                 550                 555                 560 


Pro Val Ala Phe Asn Asp Ala Ala Ala Gly Arg Glu Tyr Arg Arg Arg 
                565                 570                 575     


Gln Lys Gln Leu Lys Ser Ala Thr Lys Ala Ala Ile Lys Ala Gln Lys 
            580                 585                 590         


Gln Met Asp Ala Leu Glu Val Ala Glu Leu Leu Pro Gln Ile Ala Glu 
        595                 600                 605             


Pro Glu Ala Pro Glu Ser Arg Ile Val Gly Ile Phe Arg Pro Ser Gly 
    610                 615                 620                 


Asn Thr Glu Arg Val Lys Asn Gln Glu Arg Asp Asp Glu Tyr Glu Thr 
625                 630                 635                 640 


Glu Arg Asp Glu Tyr Leu Asn His Ser Leu Asp Ile Leu Glu Gln Asn 
                645                 650                 655     


Arg Arg Lys Lys Ala Ile 
            660         


<210>  3
<211>  326
<212>  PRT
<213>  Sus scrofa

<400>  3

Ile Gly Asp Glu Pro Leu Glu Asn Tyr Leu Asp Thr Glu Tyr Phe Gly 
1               5                   10                  15      


Thr Ile Gly Ile Gly Thr Pro Ala Gln Asp Phe Thr Val Ile Phe Asp 
            20                  25                  30          


Thr Gly Ser Ser Asn Leu Trp Val Pro Ser Val Tyr Cys Ser Ser Leu 
        35                  40                  45              


Ala Cys Ser Asp His Asn Gln Phe Asn Pro Asp Asp Ser Ser Thr Phe 
    50                  55                  60                  


Glu Ala Thr Ser Gln Glu Leu Ser Ile Thr Tyr Gly Thr Gly Ser Met 
65                  70                  75                  80  


Thr Gly Ile Leu Gly Tyr Asp Thr Val Gln Val Gly Gly Ile Ser Asp 
                85                  90                  95      


Thr Asn Gln Ile Phe Gly Leu Ser Glu Thr Glu Pro Gly Ser Phe Leu 
            100                 105                 110         


Tyr Tyr Ala Pro Phe Asp Gly Ile Leu Gly Leu Ala Tyr Pro Ser Ile 
        115                 120                 125             


Ser Ala Ser Gly Ala Thr Pro Val Phe Asp Asn Leu Trp Asp Gln Gly 
    130                 135                 140                 


Leu Val Ser Gln Asp Leu Phe Ser Val Tyr Leu Ser Ser Asn Asp Asp 
145                 150                 155                 160 


Ser Gly Ser Val Val Leu Leu Gly Gly Ile Asp Ser Ser Tyr Tyr Thr 
                165                 170                 175     


Gly Ser Leu Asn Trp Val Pro Val Ser Val Glu Gly Tyr Trp Gln Ile 
            180                 185                 190         


Thr Leu Asp Ser Ile Thr Met Asp Gly Glu Thr Ile Ala Cys Ser Gly 
        195                 200                 205             


Gly Cys Gln Ala Ile Val Asp Thr Gly Thr Ser Leu Leu Thr Gly Pro 
    210                 215                 220                 


Thr Ser Ala Ile Ala Asn Ile Gln Ser Asp Ile Gly Ala Ser Glu Asn 
225                 230                 235                 240 


Ser Asp Gly Glu Met Val Ile Ser Cys Ser Ser Ile Asp Ser Leu Pro 
                245                 250                 255     


Asp Ile Val Phe Thr Ile Asn Gly Val Gln Tyr Pro Leu Ser Pro Ser 
            260                 265                 270         


Ala Tyr Ile Leu Gln Asp Asp Asp Ser Cys Thr Ser Gly Phe Glu Gly 
        275                 280                 285             


Met Asp Val Pro Thr Ser Ser Gly Glu Leu Trp Ile Leu Gly Asp Val 
    290                 295                 300                 


Phe Ile Arg Gln Tyr Tyr Thr Val Phe Asp Arg Ala Asn Asn Lys Val 
305                 310                 315                 320 


Gly Leu Ala Pro Val Ala 
                325     


<210>  4
<211>  330
<212>  PRT
<213>  Bos taurus

<400>  4

Ala Ala Thr Leu Val Ser Glu Gln Pro Leu Gln Asn Tyr Leu Asp Thr 
1               5                   10                  15      


Glu Tyr Phe Gly Thr Ile Gly Ile Gly Thr Pro Ala Gln Asp Phe Thr 
            20                  25                  30          


Val Ile Phe Asp Thr Gly Ser Ser Asn Leu Trp Val Pro Ser Ile Tyr 
        35                  40                  45              


Cys Ser Ser Glu Ala Cys Thr Asn His Asn Arg Phe Asn Pro Gln Asp 
    50                  55                  60                  


Ser Ser Thr Tyr Glu Ala Thr Ser Glu Thr Leu Ser Ile Thr Tyr Gly 
65                  70                  75                  80  


Thr Gly Ser Met Thr Gly Ile Leu Gly Tyr Asp Thr Val Gln Val Gly 
                85                  90                  95      


Gly Ile Ser Asp Thr Asn Gln Ile Phe Gly Leu Ser Glu Thr Glu Pro 
            100                 105                 110         


Gly Ser Phe Leu Tyr Tyr Ala Pro Phe Asp Gly Ile Leu Gly Leu Ala 
        115                 120                 125             


Tyr Pro Ser Ile Ser Ser Ser Gly Ala Thr Pro Val Phe Asp Asn Ile 
    130                 135                 140                 


Trp Asp Gln Gly Leu Val Ser Gln Asp Leu Phe Ser Val Tyr Leu Ser 
145                 150                 155                 160 


Ser Asn Glu Glu Ser Gly Ser Val Val Ile Phe Gly Asp Ile Asp Ser 
                165                 170                 175     


Ser Tyr Tyr Ser Gly Ser Leu Asn Trp Val Pro Val Ser Val Glu Gly 
            180                 185                 190         


Tyr Trp Gln Ile Thr Val Asp Ser Ile Thr Met Asn Gly Glu Ser Ile 
        195                 200                 205             


Ala Cys Ser Asp Gly Cys Gln Ala Ile Val Asp Thr Gly Thr Ser Leu 
    210                 215                 220                 


Leu Ala Gly Pro Thr Thr Ala Ile Ser Asn Ile Gln Ser Tyr Ile Gly 
225                 230                 235                 240 


Ala Ser Glu Asp Ser Ser Gly Glu Val Val Ile Ser Cys Ser Ser Ile 
                245                 250                 255     


Asp Ser Leu Pro Asp Ile Val Phe Thr Ile Asn Gly Val Gln Tyr Pro 
            260                 265                 270         


Val Pro Pro Ser Ala Tyr Ile Leu Gln Ser Asn Gly Ile Cys Ser Ser 
        275                 280                 285             


Gly Phe Glu Gly Met Asp Ile Ser Thr Ser Ser Gly Asp Leu Trp Ile 
    290                 295                 300                 


Leu Gly Asp Val Phe Ile Arg Gln Tyr Phe Thr Val Phe Asp Arg Gly 
305                 310                 315                 320 


Asn Asn Gln Ile Gly Leu Ala Pro Val Ala 
                325                 330 


<210>  5
<211>  1008
<212>  PRT
<213>  Clostridium histolyticum

<400>  5

Ile Ala Asn Thr Asn Ser Glu Lys Tyr Asp Phe Glu Tyr Leu Asn Gly 
1               5                   10                  15      


Leu Ser Tyr Thr Glu Leu Thr Asn Leu Ile Lys Asn Ile Lys Trp Asn 
            20                  25                  30          


Gln Ile Asn Gly Leu Phe Asn Tyr Ser Thr Gly Ser Gln Lys Phe Phe 
        35                  40                  45              


Gly Asp Lys Asn Arg Val Gln Ala Ile Ile Asn Ala Leu Gln Glu Ser 
    50                  55                  60                  


Gly Arg Thr Tyr Thr Ala Asn Asp Met Lys Gly Ile Glu Thr Phe Thr 
65                  70                  75                  80  


Glu Val Leu Arg Ala Gly Phe Tyr Leu Gly Tyr Tyr Asn Asp Gly Leu 
                85                  90                  95      


Ser Tyr Leu Asn Asp Arg Asn Phe Gln Asp Lys Cys Ile Pro Ala Met 
            100                 105                 110         


Ile Ala Ile Gln Lys Asn Pro Asn Phe Lys Leu Gly Thr Ala Val Gln 
        115                 120                 125             


Asp Glu Val Ile Thr Ser Leu Gly Lys Leu Ile Gly Asn Ala Ser Ala 
    130                 135                 140                 


Asn Ala Glu Val Val Asn Asn Cys Val Pro Val Leu Lys Gln Phe Arg 
145                 150                 155                 160 


Glu Asn Leu Asn Gln Tyr Ala Pro Asp Tyr Val Lys Gly Thr Ala Val 
                165                 170                 175     


Asn Glu Leu Ile Lys Gly Ile Glu Phe Asp Phe Ser Gly Ala Ala Tyr 
            180                 185                 190         


Glu Lys Asp Val Lys Thr Met Pro Trp Tyr Gly Lys Ile Asp Pro Phe 
        195                 200                 205             


Ile Asn Glu Leu Lys Ala Leu Gly Leu Tyr Gly Asn Ile Thr Ser Ala 
    210                 215                 220                 


Thr Glu Trp Ala Ser Asp Val Gly Ile Tyr Tyr Leu Ser Lys Phe Gly 
225                 230                 235                 240 


Leu Tyr Ser Thr Asn Arg Asn Asp Ile Val Gln Ser Leu Glu Lys Ala 
                245                 250                 255     


Val Asp Met Tyr Lys Tyr Gly Lys Ile Ala Phe Val Ala Met Glu Arg 
            260                 265                 270         


Ile Thr Trp Asp Tyr Asp Gly Ile Gly Ser Asn Gly Lys Lys Val Asp 
        275                 280                 285             


His Asp Lys Phe Leu Asp Asp Ala Glu Lys His Tyr Leu Pro Lys Thr 
    290                 295                 300                 


Tyr Thr Phe Asp Asn Gly Thr Phe Ile Ile Arg Ala Gly Asp Lys Val 
305                 310                 315                 320 


Ser Glu Glu Lys Ile Lys Arg Leu Tyr Trp Ala Ser Arg Glu Val Lys 
                325                 330                 335     


Ser Gln Phe His Arg Val Val Gly Asn Asp Lys Ala Leu Glu Val Gly 
            340                 345                 350         


Asn Ala Asp Asp Val Leu Thr Met Lys Ile Phe Asn Ser Pro Glu Glu 
        355                 360                 365             


Tyr Lys Phe Asn Thr Asn Ile Asn Gly Val Ser Thr Asp Asn Gly Gly 
    370                 375                 380                 


Leu Tyr Ile Glu Pro Arg Gly Thr Phe Tyr Thr Tyr Glu Arg Thr Pro 
385                 390                 395                 400 


Gln Gln Ser Ile Phe Ser Leu Glu Glu Leu Phe Arg His Glu Tyr Thr 
                405                 410                 415     


His Tyr Leu Gln Ala Arg Tyr Leu Val Asp Gly Leu Trp Gly Gln Gly 
            420                 425                 430         


Pro Phe Tyr Glu Lys Asn Arg Leu Thr Trp Phe Asp Glu Gly Thr Ala 
        435                 440                 445             


Glu Phe Phe Ala Gly Ser Thr Arg Thr Ser Gly Val Leu Pro Arg Lys 
    450                 455                 460                 


Ser Ile Leu Gly Tyr Leu Ala Lys Asp Lys Val Asp His Arg Tyr Ser 
465                 470                 475                 480 


Leu Lys Lys Thr Leu Asn Ser Gly Tyr Asp Asp Ser Asp Trp Met Phe 
                485                 490                 495     


Tyr Asn Tyr Gly Phe Ala Val Ala His Tyr Leu Tyr Glu Lys Asp Met 
            500                 505                 510         


Pro Thr Phe Ile Lys Met Asn Lys Ala Ile Leu Asn Thr Asp Val Lys 
        515                 520                 525             


Ser Tyr Asp Glu Ile Ile Lys Lys Leu Ser Asp Asp Ala Asn Lys Asn 
    530                 535                 540                 


Thr Glu Tyr Gln Asn His Ile Gln Glu Leu Ala Asp Lys Tyr Gln Gly 
545                 550                 555                 560 


Ala Gly Ile Pro Leu Val Ser Asp Asp Tyr Leu Lys Asp His Gly Tyr 
                565                 570                 575     


Lys Lys Ala Ser Glu Val Tyr Ser Glu Ile Ser Lys Ala Ala Ser Leu 
            580                 585                 590         


Thr Asn Thr Ser Val Thr Ala Glu Lys Ser Gln Tyr Phe Asn Thr Phe 
        595                 600                 605             


Thr Leu Arg Gly Thr Tyr Thr Gly Glu Thr Ser Lys Gly Glu Phe Lys 
    610                 615                 620                 


Asp Trp Asp Glu Met Ser Lys Lys Leu Asp Gly Thr Leu Glu Ser Leu 
625                 630                 635                 640 


Ala Lys Asn Ser Trp Ser Gly Tyr Lys Thr Leu Thr Ala Tyr Phe Thr 
                645                 650                 655     


Asn Tyr Arg Val Thr Ser Asp Asn Lys Val Gln Tyr Asp Val Val Phe 
            660                 665                 670         


His Gly Val Leu Thr Asp Asn Ala Asp Ile Ser Asn Asn Lys Ala Pro 
        675                 680                 685             


Ile Ala Lys Val Thr Gly Pro Ser Thr Gly Ala Val Gly Arg Asn Ile 
    690                 695                 700                 


Glu Phe Ser Gly Lys Asp Ser Lys Asp Glu Asp Gly Lys Ile Val Ser 
705                 710                 715                 720 


Tyr Asp Trp Asp Phe Gly Asp Gly Ala Thr Ser Arg Gly Lys Asn Ser 
                725                 730                 735     


Val His Ala Tyr Lys Lys Ala Gly Thr Tyr Asn Val Thr Leu Lys Val 
            740                 745                 750         


Thr Asp Asp Lys Gly Ala Thr Ala Thr Glu Ser Phe Thr Ile Glu Ile 
        755                 760                 765             


Lys Asn Glu Asp Thr Thr Thr Pro Ile Thr Lys Glu Met Glu Pro Asn 
    770                 775                 780                 


Asp Asp Ile Lys Glu Ala Asn Gly Pro Ile Val Glu Gly Val Thr Val 
785                 790                 795                 800 


Lys Gly Asp Leu Asn Gly Ser Asp Asp Ala Asp Thr Phe Tyr Phe Asp 
                805                 810                 815     


Val Lys Glu Asp Gly Asp Val Thr Ile Glu Leu Pro Tyr Ser Gly Ser 
            820                 825                 830         


Ser Asn Phe Thr Trp Leu Val Tyr Lys Glu Gly Asp Asp Gln Asn His 
        835                 840                 845             


Ile Ala Ser Gly Ile Asp Lys Asn Asn Ser Lys Val Gly Thr Phe Lys 
    850                 855                 860                 


Ser Thr Lys Gly Arg His Tyr Val Phe Ile Tyr Lys His Asp Ser Ala 
865                 870                 875                 880 


Ser Asn Ile Ser Tyr Ser Leu Asn Ile Lys Gly Leu Gly Asn Glu Lys 
                885                 890                 895     


Leu Lys Glu Lys Glu Asn Asn Asp Ser Ser Asp Lys Ala Thr Val Ile 
            900                 905                 910         


Pro Asn Phe Asn Thr Thr Met Gln Gly Ser Leu Leu Gly Asp Asp Ser 
        915                 920                 925             


Arg Asp Tyr Tyr Ser Phe Glu Val Lys Glu Glu Gly Glu Val Asn Ile 
    930                 935                 940                 


Glu Leu Asp Lys Lys Asp Glu Phe Gly Val Thr Trp Thr Leu His Pro 
945                 950                 955                 960 


Glu Ser Asn Ile Asn Asp Arg Ile Thr Tyr Gly Gln Val Asp Gly Asn 
                965                 970                 975     


Lys Val Ser Asn Lys Val Lys Leu Arg Pro Gly Lys Tyr Tyr Leu Leu 
            980                 985                 990         


Val Tyr Lys Tyr Ser Gly Ser Gly  Asn Tyr Glu Leu Arg  Val Asn Lys 
        995                 1000                 1005             


<210>  6
<211>  981
<212>  PRT
<213>  Clostridium histolyticum

<400>  6

Val Gln Asn Glu Ser Lys Arg Tyr Thr Val Ser Tyr Leu Lys Thr Leu 
1               5                   10                  15      


Asn Tyr Tyr Asp Leu Val Asp Leu Leu Val Lys Thr Glu Ile Glu Asn 
            20                  25                  30          


Leu Pro Asp Leu Phe Gln Tyr Ser Ser Asp Ala Lys Glu Phe Tyr Gly 
        35                  40                  45              


Asn Lys Thr Arg Met Ser Phe Ile Met Asp Glu Ile Gly Arg Arg Ala 
    50                  55                  60                  


Pro Gln Tyr Thr Glu Ile Asp His Lys Gly Ile Pro Thr Leu Val Glu 
65                  70                  75                  80  


Val Val Arg Ala Gly Phe Tyr Leu Gly Phe His Asn Lys Glu Leu Asn 
                85                  90                  95      


Glu Ile Asn Lys Arg Ser Phe Lys Glu Arg Val Ile Pro Ser Ile Leu 
            100                 105                 110         


Ala Ile Gln Lys Asn Pro Asn Phe Lys Leu Gly Thr Glu Val Gln Asp 
        115                 120                 125             


Lys Ile Val Ser Ala Thr Gly Leu Leu Ala Gly Asn Glu Thr Ala Pro 
    130                 135                 140                 


Pro Glu Val Val Asn Asn Phe Thr Pro Ile Leu Gln Asp Cys Ile Lys 
145                 150                 155                 160 


Asn Ile Asp Arg Tyr Ala Leu Asp Asp Leu Lys Ser Lys Ala Leu Phe 
                165                 170                 175     


Asn Val Leu Ala Ala Pro Thr Tyr Asp Ile Thr Glu Tyr Leu Arg Ala 
            180                 185                 190         


Thr Lys Glu Lys Pro Glu Asn Thr Pro Trp Tyr Gly Lys Ile Asp Gly 
        195                 200                 205             


Phe Ile Asn Glu Leu Lys Lys Leu Ala Leu Tyr Gly Lys Ile Asn Asp 
    210                 215                 220                 


Asn Asn Ser Trp Ile Ile Asp Asn Gly Ile Tyr His Ile Ala Pro Leu 
225                 230                 235                 240 


Gly Lys Leu His Ser Asn Asn Lys Ile Gly Ile Glu Thr Leu Thr Glu 
                245                 250                 255     


Val Met Lys Val Tyr Pro Tyr Leu Ser Met Gln His Leu Gln Ser Ala 
            260                 265                 270         


Asp Gln Ile Lys Arg His Tyr Asp Ser Lys Asp Ala Glu Gly Asn Lys 
        275                 280                 285             


Ile Pro Leu Asp Lys Phe Lys Lys Glu Gly Lys Glu Lys Tyr Cys Pro 
    290                 295                 300                 


Lys Thr Tyr Thr Phe Asp Asp Gly Lys Val Ile Ile Lys Ala Gly Ala 
305                 310                 315                 320 


Arg Val Glu Glu Glu Lys Val Lys Arg Leu Tyr Trp Ala Ser Lys Glu 
                325                 330                 335     


Val Asn Ser Gln Phe Phe Arg Val Tyr Gly Ile Asp Lys Pro Leu Glu 
            340                 345                 350         


Glu Gly Asn Pro Asp Asp Ile Leu Thr Met Val Ile Tyr Asn Ser Pro 
        355                 360                 365             


Glu Glu Tyr Lys Leu Asn Ser Val Leu Tyr Gly Tyr Asp Thr Asn Asn 
    370                 375                 380                 


Gly Gly Met Tyr Ile Glu Pro Glu Gly Thr Phe Phe Thr Tyr Glu Arg 
385                 390                 395                 400 


Glu Ala Gln Glu Ser Thr Tyr Thr Leu Glu Glu Leu Phe Arg His Glu 
                405                 410                 415     


Tyr Thr His Tyr Leu Gln Gly Arg Tyr Ala Val Pro Gly Gln Trp Gly 
            420                 425                 430         


Arg Thr Lys Leu Tyr Asp Asn Asp Arg Leu Thr Trp Tyr Glu Glu Gly 
        435                 440                 445             


Gly Ala Glu Leu Phe Ala Gly Ser Thr Arg Thr Ser Gly Ile Leu Pro 
    450                 455                 460                 


Arg Lys Ser Ile Val Ser Asn Ile His Asn Thr Thr Arg Asn Asn Arg 
465                 470                 475                 480 


Tyr Lys Leu Ser Asp Thr Val His Ser Lys Tyr Gly Ala Ser Phe Glu 
                485                 490                 495     


Phe Tyr Asn Tyr Ala Cys Met Phe Met Asp Tyr Met Tyr Asn Lys Asp 
            500                 505                 510         


Met Gly Ile Leu Asn Lys Leu Asn Asp Leu Ala Lys Asn Asn Asp Val 
        515                 520                 525             


Asp Gly Tyr Asp Asn Tyr Ile Arg Asp Leu Ser Ser Asn Tyr Ala Leu 
    530                 535                 540                 


Asn Asp Lys Tyr Gln Asp His Met Gln Glu Arg Ile Asp Asn Tyr Glu 
545                 550                 555                 560 


Asn Leu Thr Val Pro Phe Val Ala Asp Asp Tyr Leu Val Arg His Ala 
                565                 570                 575     


Tyr Lys Asn Pro Asn Glu Ile Tyr Ser Glu Ile Ser Glu Val Ala Lys 
            580                 585                 590         


Leu Lys Asp Ala Lys Ser Glu Val Lys Lys Ser Gln Tyr Phe Ser Thr 
        595                 600                 605             


Phe Thr Leu Arg Gly Ser Tyr Thr Gly Gly Ala Ser Lys Gly Lys Leu 
    610                 615                 620                 


Glu Asp Gln Lys Ala Met Asn Lys Phe Ile Asp Asp Ser Leu Lys Lys 
625                 630                 635                 640 


Leu Asp Thr Tyr Ser Trp Ser Gly Tyr Lys Thr Leu Thr Ala Tyr Phe 
                645                 650                 655     


Thr Asn Tyr Lys Val Asp Ser Ser Asn Arg Val Thr Tyr Asp Val Val 
            660                 665                 670         


Phe His Gly Tyr Leu Pro Asn Glu Gly Asp Ser Lys Asn Ser Leu Pro 
        675                 680                 685             


Tyr Gly Lys Ile Asn Gly Thr Tyr Lys Gly Thr Glu Lys Glu Lys Ile 
    690                 695                 700                 


Lys Phe Ser Ser Glu Gly Ser Phe Asp Pro Asp Gly Lys Ile Val Ser 
705                 710                 715                 720 


Tyr Glu Trp Asp Phe Gly Asp Gly Asn Lys Ser Asn Glu Glu Asn Pro 
                725                 730                 735     


Glu His Ser Tyr Asp Lys Val Gly Thr Tyr Thr Val Lys Leu Lys Val 
            740                 745                 750         


Thr Asp Asp Lys Gly Glu Ser Ser Val Ser Thr Thr Thr Ala Glu Ile 
        755                 760                 765             


Lys Asp Leu Ser Glu Asn Lys Leu Pro Val Ile Tyr Met His Val Pro 
    770                 775                 780                 


Lys Ser Gly Ala Leu Asn Gln Lys Val Val Phe Tyr Gly Lys Gly Thr 
785                 790                 795                 800 


Tyr Asp Pro Asp Gly Ser Ile Ala Gly Tyr Gln Trp Asp Phe Gly Asp 
                805                 810                 815     


Gly Ser Asp Phe Ser Ser Glu Gln Asn Pro Ser His Val Tyr Thr Lys 
            820                 825                 830         


Lys Gly Glu Tyr Thr Val Thr Leu Arg Val Met Asp Ser Ser Gly Gln 
        835                 840                 845             


Met Ser Glu Lys Thr Met Lys Ile Lys Ile Thr Asp Pro Val Tyr Pro 
    850                 855                 860                 


Ile Gly Thr Glu Lys Glu Pro Asn Asn Ser Lys Glu Thr Ala Ser Gly 
865                 870                 875                 880 


Pro Ile Val Pro Gly Ile Pro Val Ser Gly Thr Ile Glu Asn Thr Ser 
                885                 890                 895     


Asp Gln Asp Tyr Phe Tyr Phe Asp Val Ile Thr Pro Gly Glu Val Lys 
            900                 905                 910         


Ile Asp Ile Asn Lys Leu Gly Tyr Gly Gly Ala Thr Trp Val Val Tyr 
        915                 920                 925             


Asp Glu Asn Asn Asn Ala Val Ser Tyr Ala Thr Asp Asp Gly Gln Asn 
    930                 935                 940                 


Leu Ser Gly Lys Phe Lys Ala Asp Lys Pro Gly Arg Tyr Tyr Ile His 
945                 950                 955                 960 


Leu Tyr Met Phe Asn Gly Ser Tyr Met Pro Tyr Arg Ile Asn Ile Glu 
                965                 970                 975     


Gly Ser Val Gly Arg 
            980     


<210>  7
<211>  279
<212>  PRT
<213>  Tritirachium album

<400>  7

Ala Ala Gln Thr Asn Ala Pro Trp Gly Leu Ala Arg Ile Ser Ser Thr 
1               5                   10                  15      


Ser Pro Gly Thr Ser Thr Tyr Tyr Tyr Asp Glu Ser Ala Gly Gln Gly 
            20                  25                  30          


Ser Cys Val Tyr Val Ile Asp Thr Gly Ile Glu Ala Ser His Pro Glu 
        35                  40                  45              


Phe Glu Gly Arg Ala Gln Met Val Lys Thr Tyr Tyr Tyr Ser Ser Arg 
    50                  55                  60                  


Asp Gly Asn Gly His Gly Thr His Cys Ala Gly Thr Val Gly Ser Arg 
65                  70                  75                  80  


Thr Tyr Gly Val Ala Lys Lys Thr Gln Leu Phe Gly Val Lys Val Leu 
                85                  90                  95      


Asp Asp Asn Gly Ser Gly Gln Tyr Ser Thr Ile Ile Ala Gly Met Asp 
            100                 105                 110         


Phe Val Ala Ser Asp Lys Asn Asn Arg Asn Cys Pro Lys Gly Val Val 
        115                 120                 125             


Ala Ser Leu Ser Leu Gly Gly Gly Tyr Ser Ser Ser Val Asn Ser Ala 
    130                 135                 140                 


Ala Ala Arg Leu Gln Ser Ser Gly Val Met Val Ala Val Ala Ala Gly 
145                 150                 155                 160 


Asn Asn Asn Ala Asp Ala Arg Asn Tyr Ser Pro Ala Ser Glu Pro Ser 
                165                 170                 175     


Val Cys Thr Val Gly Ala Ser Asp Arg Tyr Asp Arg Arg Ser Ser Phe 
            180                 185                 190         


Ser Asn Tyr Gly Ser Val Leu Asp Ile Phe Gly Pro Gly Thr Ser Ile 
        195                 200                 205             


Leu Ser Thr Trp Ile Gly Gly Ser Thr Arg Ser Ile Ser Gly Thr Ser 
    210                 215                 220                 


Met Ala Thr Pro His Val Ala Gly Leu Ala Ala Tyr Leu Met Thr Leu 
225                 230                 235                 240 


Gly Lys Thr Thr Ala Ala Ser Ala Cys Arg Tyr Ile Ala Asp Thr Ala 
                245                 250                 255     


Asn Lys Gly Asp Leu Ser Asn Ile Pro Phe Gly Thr Val Asn Leu Leu 
            260                 265                 270         


Ala Tyr Asn Asn Tyr Gln Ala 
        275                 


<210>  8
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Tn5 Mosaic end sequence

<400>  8
ctgtctctta tacacatct                                                    19


<210>  9
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mu transposase recognition sequence

<400>  9
tgaagcggcg cacgaaaaac gcgaaag                                           27


<210>  10
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mu transposase recognition sequence

<400>  10
gcgtttcacg ataaatgcga aaa                                               23


<210>  11
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mu transposase recognition sequence

<400>  11
ctgtttcatt tgaagcgcga aag                                               23


<210>  12
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mu transposase recognition sequence

<400>  12
tgtattgatt cacttgaagt acgaaaa                                           27


<210>  13
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mu transposase recognition sequence

<400>  13
ccttaatcaa tgaaacgcga aag                                               23


<210>  14
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mu transposase recognition sequence

<400>  14
ttgtttcatt gaaaatacga aaa                                               23


<210>  15
<211>  79
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Surface probe of the capture probe


<220>
<221>  misc_feature
<222>  (52)..(59)
<223>  n is a, c, g, t or u

<400>  15
uuuuugactc gtaatacgac tcactatagg gacacgacgc tcttccgatc tnnnnnnnnt       60

gcacgcggtg tacagacgt                                                    79


<210>  16
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  First Adapter

<400>  16
gtctcgtggg ctcgg                                                        15


<210>  17
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Capture Domain

<400>  17
ccgagcccac gagac                                                        15


<210>  18
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Hybridization Domain

<400>  18
tgcacgcggt gtacagacgt                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Splint oligonucleotide complementary to hybridization domain

<400>  19
acgtctgtac accgcgtgca                                                   20


<210>  20
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Second adapter

<400>  20
tcgtcggcag cgtc                                                         14


<210>  21
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  A-Short forward

<400>  21
acacgacgct cttccgatct                                                   20


<210>  22
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic trademark of PURAMATRIX polypeptide sequence

<400>  22

Arg Ala Asp Ala Arg Ala Asp Ala Arg Ala Asp Ala Arg Ala Asp Ala 
1               5                   10                  15      


<210>  23
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic EAK16 polypeptide sequence

<400>  23

Ala Glu Ala Glu Ala Lys Ala Lys Ala Glu Ala Glu Ala Lys Ala Lys 
1               5                   10                  15      


<210>  24
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic KLD12 polypeptide sequence

<400>  24

Lys Leu Asp Leu Lys Leu Asp Leu Lys Leu Asp Leu 
1               5                   10          


<210>  25
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Poly(A) tail added to 3' ends of tagmented DNA

<400>  25
aaaaaaaaaa                                                              10


<210>  26
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Capture probe

<400>  26

Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr 
1               5                   10                  15      


Thr Thr Thr Thr 
            20  


