                               SEQUENCE LISTING

<110> NATIONAL UNIVERSITY OF SINGAPORE
 
<120> A METHOD TO SPECIFICALLY STIMULATE SURVIVAL AND EXPANSION OF 
      GENETICALLY-MODIFIED IMMUNE CELLS

<130> 4459.1151-001 PCT

<140>
<141>

<150> 62/724,488
<151> 2018-08-29

<160> 27    

<170> PatentIn version 3.5

<210> 1
<211> 1551
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      Epo receptor (EpoR) cDNA sequence

<400> 1
ccggaattcg ccaccatgga ccacctcggg gcgtccctct ggccccaggt cggctccctt       60

tgtctcctgc tcgctggggc cgcctgggcg cccccgccta acctcccgga ccccaagttc      120

gagagcaaag cggccttgct ggcggcccgg gggcccgaag agcttctgtg cttcaccgag      180

cggttggagg acttggtgtg tttctgggag gaagcggcga gcgctggggt gggcccgggc      240

aactacagct tctcctacca gttagaagat gagccatgga agctgtgtcg cctgcaccag      300

gctcccacgg ctcgtggtgc ggtgcgcttc tggtgttcgc tgcctacagc cgacacgtcg      360

agcttcgtgc ccctagagtt gcgcgtcaca gcagcctccg gcgctccgcg atatcaccgt      420

gtcatccaca tcaatgaagt agtgctccta gacgcccccg tggggctggt ggcgcggttg      480

gctgacgaga gcggccacgt agtgttgcgc tggctcccgc cgcctgagac acccatgacg      540

tctcacatcc gctacgaggt ggacgtctcg gccggcaacg gcgcagggag cgtacagagg      600

gtggagatcc tggagggccg caccgagtgt gtgctgagca acctgcgggg ccggacgcgc      660

tacaccttcg ccgtccgcgc gcgtatggct gagccgagct tcggcggctt ctggagcgcc      720

tggtcggagc ctgtgtcgct gctgacgcct agcgacctgg accccctcat cctgacgctc      780

tccctcatcc tcgtggtcat cctggtgctg ctgaccgtgc tcgcgctgct ctcccaccgc      840

cgggctctga agcagaagat ctggcctggc atcccgagcc cagagagcga gtttgaaggc      900

ctcttcacca cccacaaggg taacttccag ctgtggctgt accagaatga tggctgcctg      960

tggtggagcc cctgcacccc cttcacggag gacccacctg cttccctgga agtcctctca     1020

gagcgctgct gggggacgat gcaggcagtg gagccgggga cagatgatga gggccccctg     1080

ctggagccag tgggcagtga gcatgcccag gatacctatc tggtgctgga caaatggttg     1140

ctgccccgga acccgcccag tgaggacctc ccagggcctg gtggcagtgt ggacatagtg     1200

gccatggatg aaggctcaga agcatcctcc tgctcatctg ctttggcctc gaagcccagc     1260

ccagagggag cctctgctgc cagctttgag tacactatcc tggaccccag ctcccagctc     1320

ttgcgtccat ggacactgtg ccctgagctg ccccctaccc caccccacct aaagtacctg     1380

taccttgtgg tatctgactc tggcatctca actgactaca gctcagggga ctcccaggga     1440

gcccaagggg gcttatccga tggcccctac tccaaccctt atgagaacag ccttatccca     1500

gccgctgagc ctctgccccc cagctatgtg gcttgctctt agctcgagcg g              1551


<210> 2
<211> 508
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      Epo receptor (EpoR) amino acid sequence

<400> 2
Met Asp His Leu Gly Ala Ser Leu Trp Pro Gln Val Gly Ser Leu Cys 
1               5                   10                  15      


Leu Leu Leu Ala Gly Ala Ala Trp Ala Pro Pro Pro Asn Leu Pro Asp 
            20                  25                  30          


Pro Lys Phe Glu Ser Lys Ala Ala Leu Leu Ala Ala Arg Gly Pro Glu 
        35                  40                  45              


Glu Leu Leu Cys Phe Thr Glu Arg Leu Glu Asp Leu Val Cys Phe Trp 
    50                  55                  60                  


Glu Glu Ala Ala Ser Ala Gly Val Gly Pro Gly Asn Tyr Ser Phe Ser 
65                  70                  75                  80  


Tyr Gln Leu Glu Asp Glu Pro Trp Lys Leu Cys Arg Leu His Gln Ala 
                85                  90                  95      


Pro Thr Ala Arg Gly Ala Val Arg Phe Trp Cys Ser Leu Pro Thr Ala 
            100                 105                 110         


Asp Thr Ser Ser Phe Val Pro Leu Glu Leu Arg Val Thr Ala Ala Ser 
        115                 120                 125             


Gly Ala Pro Arg Tyr His Arg Val Ile His Ile Asn Glu Val Val Leu 
    130                 135                 140                 


Leu Asp Ala Pro Val Gly Leu Val Ala Arg Leu Ala Asp Glu Ser Gly 
145                 150                 155                 160 


His Val Val Leu Arg Trp Leu Pro Pro Pro Glu Thr Pro Met Thr Ser 
                165                 170                 175     


His Ile Arg Tyr Glu Val Asp Val Ser Ala Gly Asn Gly Ala Gly Ser 
            180                 185                 190         


Val Gln Arg Val Glu Ile Leu Glu Gly Arg Thr Glu Cys Val Leu Ser 
        195                 200                 205             


Asn Leu Arg Gly Arg Thr Arg Tyr Thr Phe Ala Val Arg Ala Arg Met 
    210                 215                 220                 


Ala Glu Pro Ser Phe Gly Gly Phe Trp Ser Ala Trp Ser Glu Pro Val 
225                 230                 235                 240 


Ser Leu Leu Thr Pro Ser Asp Leu Asp Pro Leu Ile Leu Thr Leu Ser 
                245                 250                 255     


Leu Ile Leu Val Val Ile Leu Val Leu Leu Thr Val Leu Ala Leu Leu 
            260                 265                 270         


Ser His Arg Arg Ala Leu Lys Gln Lys Ile Trp Pro Gly Ile Pro Ser 
        275                 280                 285             


Pro Glu Ser Glu Phe Glu Gly Leu Phe Thr Thr His Lys Gly Asn Phe 
    290                 295                 300                 


Gln Leu Trp Leu Tyr Gln Asn Asp Gly Cys Leu Trp Trp Ser Pro Cys 
305                 310                 315                 320 


Thr Pro Phe Thr Glu Asp Pro Pro Ala Ser Leu Glu Val Leu Ser Glu 
                325                 330                 335     


Arg Cys Trp Gly Thr Met Gln Ala Val Glu Pro Gly Thr Asp Asp Glu 
            340                 345                 350         


Gly Pro Leu Leu Glu Pro Val Gly Ser Glu His Ala Gln Asp Thr Tyr 
        355                 360                 365             


Leu Val Leu Asp Lys Trp Leu Leu Pro Arg Asn Pro Pro Ser Glu Asp 
    370                 375                 380                 


Leu Pro Gly Pro Gly Gly Ser Val Asp Ile Val Ala Met Asp Glu Gly 
385                 390                 395                 400 


Ser Glu Ala Ser Ser Cys Ser Ser Ala Leu Ala Ser Lys Pro Ser Pro 
                405                 410                 415     


Glu Gly Ala Ser Ala Ala Ser Phe Glu Tyr Thr Ile Leu Asp Pro Ser 
            420                 425                 430         


Ser Gln Leu Leu Arg Pro Trp Thr Leu Cys Pro Glu Leu Pro Pro Thr 
        435                 440                 445             


Pro Pro His Leu Lys Tyr Leu Tyr Leu Val Val Ser Asp Ser Gly Ile 
    450                 455                 460                 


Ser Thr Asp Tyr Ser Ser Gly Asp Ser Gln Gly Ala Gln Gly Gly Leu 
465                 470                 475                 480 


Ser Asp Gly Pro Tyr Ser Asn Pro Tyr Glu Asn Ser Leu Ile Pro Ala 
                485                 490                 495     


Ala Glu Pro Leu Pro Pro Ser Tyr Val Ala Cys Ser 
            500                 505             


<210> 3
<211> 1581
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      EpoR with Flag tag cDNA sequence

<400> 3
atatatgaat tcgccaccat ggaccacctc ggggcgtccc tctggcccca ggtcggctcc       60

ctttgtctcc tgctcgctgg ggccgcctgg gcgcccccgc ctaacctccc ggaccccaag      120

ttcgagagca aagcggcctt gctggcggcc cgggggcccg aagagcttct gtgcttcacc      180

gagcggttgg aggacttggt gtgtttctgg gaggaagcgg cgagcgctgg ggtgggcccg      240

ggcaactaca gcttctccta ccagttagaa gatgagccat ggaagctgtg tcgcctgcac      300

caggctccca cggctcgtgg tgcggtgcgc ttctggtgtt cgctgcctac agccgacacg      360

tcgagcttcg tgcccctaga gttgcgcgtc acagcagcct ccggcgctcc gcgatatcac      420

cgtgtcatcc acatcaatga agtagtgctc ctagacgccc ccgtggggct ggtggcgcgg      480

ttggctgacg agagcggcca cgtagtgttg cgctggctcc cgccgcctga gacacccatg      540

acgtctcaca tccgctacga ggtggacgtc tcggccggca acggcgcagg gagcgtacag      600

agggtggaga tcctggaggg ccgcaccgag tgtgtgctga gcaacctgcg gggccggacg      660

cgctacacct tcgccgtccg cgcgcgtatg gctgagccga gcttcggcgg cttctggagc      720

gcctggtcgg agcctgtgtc gctgctgacg cctagcgacc tggaccccct catcctgacg      780

ctctccctca tcctcgtggt catcctggtg ctgctgaccg tgctcgcgct gctctcccac      840

cgccgggctc tgaagcagaa gatctggcct ggcatcccga gcccagagag cgagtttgaa      900

ggcctcttca ccacccacaa gggtaacttc cagctgtggc tgtaccagaa tgatggctgc      960

ctgtggtgga gcccctgcac ccccttcacg gaggacccac ctgcttccct ggaagtcctc     1020

tcagagcgct gctgggggac gatgcaggca gtggagccgg ggacagatga tgagggcccc     1080

ctgctggagc cagtgggcag tgagcatgcc caggatacct atctggtgct ggacaaatgg     1140

ttgctgcccc ggaacccgcc cagtgaggac ctcccagggc ctggtggcag tgtggacata     1200

gtggccatgg atgaaggctc agaagcatcc tcctgctcat ctgctttggc ctcgaagccc     1260

agcccagagg gagcctctgc tgccagcttt gagtacacta tcctggaccc cagctcccag     1320

ctcttgcgtc catggacact gtgccctgag ctgcccccta ccccacccca cctaaagtac     1380

ctgtaccttg tggtatctga ctctggcatc tcaactgact acagctcagg ggactcccag     1440

ggagcccaag ggggcttatc cgatggcccc tactccaacc cttatgagaa cagccttatc     1500

ccagccgctg agcctctgcc ccccagctat gtggcttgct ctgactacaa agacgatgac     1560

gacaagtagc tcgagtatat a                                               1581


<210> 4
<211> 516
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      EpoR with Flag tag amino acid sequence

<400> 4
Met Asp His Leu Gly Ala Ser Leu Trp Pro Gln Val Gly Ser Leu Cys 
1               5                   10                  15      


Leu Leu Leu Ala Gly Ala Ala Trp Ala Pro Pro Pro Asn Leu Pro Asp 
            20                  25                  30          


Pro Lys Phe Glu Ser Lys Ala Ala Leu Leu Ala Ala Arg Gly Pro Glu 
        35                  40                  45              


Glu Leu Leu Cys Phe Thr Glu Arg Leu Glu Asp Leu Val Cys Phe Trp 
    50                  55                  60                  


Glu Glu Ala Ala Ser Ala Gly Val Gly Pro Gly Asn Tyr Ser Phe Ser 
65                  70                  75                  80  


Tyr Gln Leu Glu Asp Glu Pro Trp Lys Leu Cys Arg Leu His Gln Ala 
                85                  90                  95      


Pro Thr Ala Arg Gly Ala Val Arg Phe Trp Cys Ser Leu Pro Thr Ala 
            100                 105                 110         


Asp Thr Ser Ser Phe Val Pro Leu Glu Leu Arg Val Thr Ala Ala Ser 
        115                 120                 125             


Gly Ala Pro Arg Tyr His Arg Val Ile His Ile Asn Glu Val Val Leu 
    130                 135                 140                 


Leu Asp Ala Pro Val Gly Leu Val Ala Arg Leu Ala Asp Glu Ser Gly 
145                 150                 155                 160 


His Val Val Leu Arg Trp Leu Pro Pro Pro Glu Thr Pro Met Thr Ser 
                165                 170                 175     


His Ile Arg Tyr Glu Val Asp Val Ser Ala Gly Asn Gly Ala Gly Ser 
            180                 185                 190         


Val Gln Arg Val Glu Ile Leu Glu Gly Arg Thr Glu Cys Val Leu Ser 
        195                 200                 205             


Asn Leu Arg Gly Arg Thr Arg Tyr Thr Phe Ala Val Arg Ala Arg Met 
    210                 215                 220                 


Ala Glu Pro Ser Phe Gly Gly Phe Trp Ser Ala Trp Ser Glu Pro Val 
225                 230                 235                 240 


Ser Leu Leu Thr Pro Ser Asp Leu Asp Pro Leu Ile Leu Thr Leu Ser 
                245                 250                 255     


Leu Ile Leu Val Val Ile Leu Val Leu Leu Thr Val Leu Ala Leu Leu 
            260                 265                 270         


Ser His Arg Arg Ala Leu Lys Gln Lys Ile Trp Pro Gly Ile Pro Ser 
        275                 280                 285             


Pro Glu Ser Glu Phe Glu Gly Leu Phe Thr Thr His Lys Gly Asn Phe 
    290                 295                 300                 


Gln Leu Trp Leu Tyr Gln Asn Asp Gly Cys Leu Trp Trp Ser Pro Cys 
305                 310                 315                 320 


Thr Pro Phe Thr Glu Asp Pro Pro Ala Ser Leu Glu Val Leu Ser Glu 
                325                 330                 335     


Arg Cys Trp Gly Thr Met Gln Ala Val Glu Pro Gly Thr Asp Asp Glu 
            340                 345                 350         


Gly Pro Leu Leu Glu Pro Val Gly Ser Glu His Ala Gln Asp Thr Tyr 
        355                 360                 365             


Leu Val Leu Asp Lys Trp Leu Leu Pro Arg Asn Pro Pro Ser Glu Asp 
    370                 375                 380                 


Leu Pro Gly Pro Gly Gly Ser Val Asp Ile Val Ala Met Asp Glu Gly 
385                 390                 395                 400 


Ser Glu Ala Ser Ser Cys Ser Ser Ala Leu Ala Ser Lys Pro Ser Pro 
                405                 410                 415     


Glu Gly Ala Ser Ala Ala Ser Phe Glu Tyr Thr Ile Leu Asp Pro Ser 
            420                 425                 430         


Ser Gln Leu Leu Arg Pro Trp Thr Leu Cys Pro Glu Leu Pro Pro Thr 
        435                 440                 445             


Pro Pro His Leu Lys Tyr Leu Tyr Leu Val Val Ser Asp Ser Gly Ile 
    450                 455                 460                 


Ser Thr Asp Tyr Ser Ser Gly Asp Ser Gln Gly Ala Gln Gly Gly Leu 
465                 470                 475                 480 


Ser Asp Gly Pro Tyr Ser Asn Pro Tyr Glu Asn Ser Leu Ile Pro Ala 
                485                 490                 495     


Ala Glu Pro Leu Pro Pro Ser Tyr Val Ala Cys Ser Asp Tyr Lys Asp 
            500                 505                 510         


Asp Asp Asp Lys 
        515     


<210> 5
<211> 1551
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      mutant EpoR (EpoRm) cDNA sequence

<400> 5
ccggaattcg ccaccatgga ccacctcggg gcgtccctct ggccccaggt cggctccctt       60

tgtctcctgc tcgctggggc cgcctgggcg cccccgccta acctcccgga ccccaagttc      120

gagagcaaag cggccttgct ggcggcccgg gggcccgaag agcttctgtg cttcaccgag      180

cggttggagg acttggtgtg tttctgggag gaagcggcga gcgctggggt gggcccgggc      240

aactacagct tctcctacca gttagaagat gagccatgga agctgtgtcg cctgcaccag      300

gctcccacgg ctcgtggtgc ggtgcgcttc tggtgttcgc tgcctacagc cgacacgtcg      360

agcttcgtgc ccctagagtt gcgcgtcaca gcagcctccg gcgctccgcg atatcaccgt      420

gtcatccaca tcaatgaagt agtgctccta gacgcccccg tggggctggt ggcgcggttg      480

gctgacgaga gcggccacgt agtgttgcgc tggctcccgc cgcctgagac acccatgacg      540

tctcacatcc gctacgaggt ggacgtctcg gccggcaacg gcgcagggag cgtacagagg      600

gtggagatcc tggagggccg caccgagtgt gtgctgagca acctgcgggg ccggacgcgc      660

tacaccttcg ccgtccgcgc gcgtatggct gagccgagct tcggcggctt ctggagcgcc      720

tggtcggagc ctgtgtcgct gctgacgcct agcgacctgg accccctcat cctgacgctc      780

tccctcatcc tcgtggtcat cctggtgctg ctgaccgtgc tcgcgctgct ctcccaccgc      840

cgggctctga agcagaagat ctggcctggc atcccgagcc cagagagcga gtttgaaggc      900

ctcttcacca cccacaaggg taacttccag ctgtggctgt accagaatga tggctgcctg      960

tggtggagcc cctgcacccc cttcacggag gacccacctg cttccctgga agtcctctca     1020

gagcgctgct gggggacgat gcaggcagtg gagccgggga cagatgatga gggccccctg     1080

ctggagccag tgggcagtga gcatgcccag gatacctatc tggtgctgga caaatggttg     1140

ctgccccgga acccgcccag tgaggacctc ccagggcctg gtggcagtgt ggacatagtg     1200

gccatggatg aaggctcaga agcatcctcc tgctcatctg ctttggcctc gaagcccagc     1260

ccagagggag cctctgctgc cagctttgag tacactatcc tggaccccag ctcccagctc     1320

ttgcgtccat agacactgtg ccctgagctg ccccctaccc caccccacct aaagtacctg     1380

taccttgtgg tatctgactc tggcatctca actgactaca gctcagggga ctcccaggga     1440

gcccaagggg gcttatccga tggcccctac tccaaccctt atgagaacag ccttatccca     1500

gccgctgagc ctctgccccc cagctatgtg gcttgctctt agctcgagcg g              1551


<210> 6
<211> 438
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      mutant EpoR (EpoRm) amino acid sequence

<400> 6
Met Asp His Leu Gly Ala Ser Leu Trp Pro Gln Val Gly Ser Leu Cys 
1               5                   10                  15      


Leu Leu Leu Ala Gly Ala Ala Trp Ala Pro Pro Pro Asn Leu Pro Asp 
            20                  25                  30          


Pro Lys Phe Glu Ser Lys Ala Ala Leu Leu Ala Ala Arg Gly Pro Glu 
        35                  40                  45              


Glu Leu Leu Cys Phe Thr Glu Arg Leu Glu Asp Leu Val Cys Phe Trp 
    50                  55                  60                  


Glu Glu Ala Ala Ser Ala Gly Val Gly Pro Gly Asn Tyr Ser Phe Ser 
65                  70                  75                  80  


Tyr Gln Leu Glu Asp Glu Pro Trp Lys Leu Cys Arg Leu His Gln Ala 
                85                  90                  95      


Pro Thr Ala Arg Gly Ala Val Arg Phe Trp Cys Ser Leu Pro Thr Ala 
            100                 105                 110         


Asp Thr Ser Ser Phe Val Pro Leu Glu Leu Arg Val Thr Ala Ala Ser 
        115                 120                 125             


Gly Ala Pro Arg Tyr His Arg Val Ile His Ile Asn Glu Val Val Leu 
    130                 135                 140                 


Leu Asp Ala Pro Val Gly Leu Val Ala Arg Leu Ala Asp Glu Ser Gly 
145                 150                 155                 160 


His Val Val Leu Arg Trp Leu Pro Pro Pro Glu Thr Pro Met Thr Ser 
                165                 170                 175     


His Ile Arg Tyr Glu Val Asp Val Ser Ala Gly Asn Gly Ala Gly Ser 
            180                 185                 190         


Val Gln Arg Val Glu Ile Leu Glu Gly Arg Thr Glu Cys Val Leu Ser 
        195                 200                 205             


Asn Leu Arg Gly Arg Thr Arg Tyr Thr Phe Ala Val Arg Ala Arg Met 
    210                 215                 220                 


Ala Glu Pro Ser Phe Gly Gly Phe Trp Ser Ala Trp Ser Glu Pro Val 
225                 230                 235                 240 


Ser Leu Leu Thr Pro Ser Asp Leu Asp Pro Leu Ile Leu Thr Leu Ser 
                245                 250                 255     


Leu Ile Leu Val Val Ile Leu Val Leu Leu Thr Val Leu Ala Leu Leu 
            260                 265                 270         


Ser His Arg Arg Ala Leu Lys Gln Lys Ile Trp Pro Gly Ile Pro Ser 
        275                 280                 285             


Pro Glu Ser Glu Phe Glu Gly Leu Phe Thr Thr His Lys Gly Asn Phe 
    290                 295                 300                 


Gln Leu Trp Leu Tyr Gln Asn Asp Gly Cys Leu Trp Trp Ser Pro Cys 
305                 310                 315                 320 


Thr Pro Phe Thr Glu Asp Pro Pro Ala Ser Leu Glu Val Leu Ser Glu 
                325                 330                 335     


Arg Cys Trp Gly Thr Met Gln Ala Val Glu Pro Gly Thr Asp Asp Glu 
            340                 345                 350         


Gly Pro Leu Leu Glu Pro Val Gly Ser Glu His Ala Gln Asp Thr Tyr 
        355                 360                 365             


Leu Val Leu Asp Lys Trp Leu Leu Pro Arg Asn Pro Pro Ser Glu Asp 
    370                 375                 380                 


Leu Pro Gly Pro Gly Gly Ser Val Asp Ile Val Ala Met Asp Glu Gly 
385                 390                 395                 400 


Ser Glu Ala Ser Ser Cys Ser Ser Ala Leu Ala Ser Lys Pro Ser Pro 
                405                 410                 415     


Glu Gly Ala Ser Ala Ala Ser Phe Glu Tyr Thr Ile Leu Asp Pro Ser 
            420                 425                 430         


Ser Gln Leu Leu Arg Pro 
        435             


<210> 7
<211> 1371
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      EpoRm with Flag tag cDNA sequence

<400> 7
atatatgaat tcgccaccat ggaccacctc ggggcgtccc tctggcccca ggtcggctcc       60

ctttgtctcc tgctcgctgg ggccgcctgg gcgcccccgc ctaacctccc ggaccccaag      120

ttcgagagca aagcggcctt gctggcggcc cgggggcccg aagagcttct gtgcttcacc      180

gagcggttgg aggacttggt gtgtttctgg gaggaagcgg cgagcgctgg ggtgggcccg      240

ggcaactaca gcttctccta ccagttagaa gatgagccat ggaagctgtg tcgcctgcac      300

caggctccca cggctcgtgg tgcggtgcgc ttctggtgtt cgctgcctac agccgacacg      360

tcgagcttcg tgcccctaga gttgcgcgtc acagcagcct ccggcgctcc gcgatatcac      420

cgtgtcatcc acatcaatga agtagtgctc ctagacgccc ccgtggggct ggtggcgcgg      480

ttggctgacg agagcggcca cgtagtgttg cgctggctcc cgccgcctga gacacccatg      540

acgtctcaca tccgctacga ggtggacgtc tcggccggca acggcgcagg gagcgtacag      600

agggtggaga tcctggaggg ccgcaccgag tgtgtgctga gcaacctgcg gggccggacg      660

cgctacacct tcgccgtccg cgcgcgtatg gctgagccga gcttcggcgg cttctggagc      720

gcctggtcgg agcctgtgtc gctgctgacg cctagcgacc tggaccccct catcctgacg      780

ctctccctca tcctcgtggt catcctggtg ctgctgaccg tgctcgcgct gctctcccac      840

cgccgggctc tgaagcagaa gatctggcct ggcatcccga gcccagagag cgagtttgaa      900

ggcctcttca ccacccacaa gggtaacttc cagctgtggc tgtaccagaa tgatggctgc      960

ctgtggtgga gcccctgcac ccccttcacg gaggacccac ctgcttccct ggaagtcctc     1020

tcagagcgct gctgggggac gatgcaggca gtggagccgg ggacagatga tgagggcccc     1080

ctgctggagc cagtgggcag tgagcatgcc caggatacct atctggtgct ggacaaatgg     1140

ttgctgcccc ggaacccgcc cagtgaggac ctcccagggc ctggtggcag tgtggacata     1200

gtggccatgg atgaaggctc agaagcatcc tcctgctcat ctgctttggc ctcgaagccc     1260

agcccagagg gagcctctgc tgccagcttt gagtacacta tcctggaccc cagctcccag     1320

ctcttgcgtc cagactacaa agacgatgac gacaagtagc tcgagtatat a              1371


<210> 8
<211> 446
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      EpoRm with Flag tag amino acid sequence

<400> 8
Met Asp His Leu Gly Ala Ser Leu Trp Pro Gln Val Gly Ser Leu Cys 
1               5                   10                  15      


Leu Leu Leu Ala Gly Ala Ala Trp Ala Pro Pro Pro Asn Leu Pro Asp 
            20                  25                  30          


Pro Lys Phe Glu Ser Lys Ala Ala Leu Leu Ala Ala Arg Gly Pro Glu 
        35                  40                  45              


Glu Leu Leu Cys Phe Thr Glu Arg Leu Glu Asp Leu Val Cys Phe Trp 
    50                  55                  60                  


Glu Glu Ala Ala Ser Ala Gly Val Gly Pro Gly Asn Tyr Ser Phe Ser 
65                  70                  75                  80  


Tyr Gln Leu Glu Asp Glu Pro Trp Lys Leu Cys Arg Leu His Gln Ala 
                85                  90                  95      


Pro Thr Ala Arg Gly Ala Val Arg Phe Trp Cys Ser Leu Pro Thr Ala 
            100                 105                 110         


Asp Thr Ser Ser Phe Val Pro Leu Glu Leu Arg Val Thr Ala Ala Ser 
        115                 120                 125             


Gly Ala Pro Arg Tyr His Arg Val Ile His Ile Asn Glu Val Val Leu 
    130                 135                 140                 


Leu Asp Ala Pro Val Gly Leu Val Ala Arg Leu Ala Asp Glu Ser Gly 
145                 150                 155                 160 


His Val Val Leu Arg Trp Leu Pro Pro Pro Glu Thr Pro Met Thr Ser 
                165                 170                 175     


His Ile Arg Tyr Glu Val Asp Val Ser Ala Gly Asn Gly Ala Gly Ser 
            180                 185                 190         


Val Gln Arg Val Glu Ile Leu Glu Gly Arg Thr Glu Cys Val Leu Ser 
        195                 200                 205             


Asn Leu Arg Gly Arg Thr Arg Tyr Thr Phe Ala Val Arg Ala Arg Met 
    210                 215                 220                 


Ala Glu Pro Ser Phe Gly Gly Phe Trp Ser Ala Trp Ser Glu Pro Val 
225                 230                 235                 240 


Ser Leu Leu Thr Pro Ser Asp Leu Asp Pro Leu Ile Leu Thr Leu Ser 
                245                 250                 255     


Leu Ile Leu Val Val Ile Leu Val Leu Leu Thr Val Leu Ala Leu Leu 
            260                 265                 270         


Ser His Arg Arg Ala Leu Lys Gln Lys Ile Trp Pro Gly Ile Pro Ser 
        275                 280                 285             


Pro Glu Ser Glu Phe Glu Gly Leu Phe Thr Thr His Lys Gly Asn Phe 
    290                 295                 300                 


Gln Leu Trp Leu Tyr Gln Asn Asp Gly Cys Leu Trp Trp Ser Pro Cys 
305                 310                 315                 320 


Thr Pro Phe Thr Glu Asp Pro Pro Ala Ser Leu Glu Val Leu Ser Glu 
                325                 330                 335     


Arg Cys Trp Gly Thr Met Gln Ala Val Glu Pro Gly Thr Asp Asp Glu 
            340                 345                 350         


Gly Pro Leu Leu Glu Pro Val Gly Ser Glu His Ala Gln Asp Thr Tyr 
        355                 360                 365             


Leu Val Leu Asp Lys Trp Leu Leu Pro Arg Asn Pro Pro Ser Glu Asp 
    370                 375                 380                 


Leu Pro Gly Pro Gly Gly Ser Val Asp Ile Val Ala Met Asp Glu Gly 
385                 390                 395                 400 


Ser Glu Ala Ser Ser Cys Ser Ser Ala Leu Ala Ser Lys Pro Ser Pro 
                405                 410                 415     


Glu Gly Ala Ser Ala Ala Ser Phe Glu Tyr Thr Ile Leu Asp Pro Ser 
            420                 425                 430         


Ser Gln Leu Leu Arg Pro Asp Tyr Lys Asp Asp Asp Asp Lys 
        435                 440                 445     


<210> 9
<211> 1545
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      anti-CD19-41BB-CD3-zeta CAR cDNA sequence

<400> 9
gaattcggct tccaccatgg ccttaccagt gaccgccttg ctcctgccgc tggccttgct       60

gctccacgcc gccaggccgg acatccagat gacacagact acatcctccc tgtctgcctc      120

tctgggagac agagtcacca tcagttgcag ggcaagtcag gacattagta aatatttaaa      180

ttggtatcag cagaaaccag atggaactgt taaactcctg atctaccata catcaagatt      240

acactcagga gtcccatcaa ggttcagtgg cagtgggtct ggaacagatt attctctcac      300

cattagcaac ctggagcaag aagatattgc cacttacttt tgccaacagg gtaatacgct      360

tccgtacacg ttcggagggg ggaccaagct ggagatcaca ggtggcggtg gctcgggcgg      420

tggtgggtcg ggtggcggcg gatctgaggt gaaactgcag gagtcaggac ctggcctggt      480

ggcgccctca cagagcctgt ccgtcacatg cactgtctca ggggtctcat tacccgacta      540

tggtgtaagc tggattcgcc agcctccacg aaagggtctg gagtggctgg gagtaatatg      600

gggtagtgaa accacatact ataattcagc tctcaaatcc agactgacca tcatcaagga      660

caactccaag agccaagttt tcttaaaaat gaacagtctg caaactgatg acacagccat      720

ttactactgt gccaaacatt attactacgg tggtagctat gctatggact actggggcca      780

aggaacctca gtcaccgtct cctcaaccac gacgccagcg ccgcgaccac caacaccggc      840

gcccaccatc gcgtcgcagc ccctgtccct gcgcccagag gcgtgccggc cagcggcggg      900

gggcgcagtg cacacgaggg ggctggactt cgcctgtgat atctacatct gggcgccctt      960

ggccgggact tgtggggtcc ttctcctgtc actggttatc accctttact gcaaacgggg     1020

cagaaagaaa ctcctgtata tattcaaaca accatttatg agaccagtac aaactactca     1080

agaggaagat ggctgtagct gccgatttcc agaagaagaa gaaggaggat gtgaactgag     1140

agtgaagttc agcaggagcg cagacgcccc cgcgtaccag cagggccaga accagctcta     1200

taacgagctc aatctaggac gaagagagga gtacgatgtt ttggacaaga gacgtggccg     1260

ggaccctgag atggggggaa agccgagaag gaagaaccct caggaaggcc tgtacaatga     1320

actgcagaaa gataagatgg cggaggccta cagtgagatt gggatgaaag gcgagcgccg     1380

gaggggcaag gggcacgatg gcctttacca gggtctcagt acagccacca aggacaccta     1440

cgacgccctt cacatgcagg ccctgccccc tcgctaacag ccaggggatt tcaccactca     1500

aaggccagac ctgcagacgc ccagattatg agacacactc gagcc                     1545


<210> 10
<211> 486
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      anti-CD19-41BB-CD3-zeta CAR amino acid sequence

<400> 10
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg 
                325                 330                 335     


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
            340                 345                 350         


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
        355                 360                 365             


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
    370                 375                 380                 


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
385                 390                 395                 400 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
                405                 410                 415     


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
            420                 425                 430         


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
        435                 440                 445             


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
    450                 455                 460                 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
465                 470                 475                 480 


Gln Ala Leu Pro Pro Arg 
                485     


<210> 11
<211> 2844
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      EpoRm-2A-CAR cDNA sequence

<400> 11
gccaccatgg accacctcgg ggcgtccctc tggccccagg tcggctccct ttgtctcctg       60

ctcgctgggg ccgcctgggc gcccccgcct aacctcccgg accccaagtt cgagagcaaa      120

gcggccttgc tggcggcccg ggggcccgaa gagcttctgt gcttcaccga gcggttggag      180

gacttggtgt gtttctggga ggaagcggcg agcgctgggg tgggcccggg caactacagc      240

ttctcctacc agttagaaga tgagccatgg aagctgtgtc gcctgcacca ggctcccacg      300

gctcgtggtg cggtgcgctt ctggtgttcg ctgcctacag ccgacacgtc gagcttcgtg      360

cccctagagt tgcgcgtcac agcagcctcc ggcgctccgc gatatcaccg tgtcatccac      420

atcaatgaag tagtgctcct agacgccccc gtggggctgg tggcgcggtt ggctgacgag      480

agcggccacg tagtgttgcg ctggctcccg ccgcctgaga cacccatgac gtctcacatc      540

cgctacgagg tggacgtctc ggccggcaac ggcgcaggga gcgtacagag ggtggagatc      600

ctggagggcc gcaccgagtg tgtgctgagc aacctgcggg gccggacgcg ctacaccttc      660

gccgtccgcg cgcgtatggc tgagccgagc ttcggcggct tctggagcgc ctggtcggag      720

cctgtgtcgc tgctgacgcc tagcgacctg gaccccctca tcctgacgct ctccctcatc      780

ctcgtggtca tcctggtgct gctgaccgtg ctcgcgctgc tctcccaccg ccgggctctg      840

aagcagaaga tctggcctgg catcccgagc ccagagagcg agtttgaagg cctcttcacc      900

acccacaagg gtaacttcca gctgtggctg taccagaatg atggctgcct gtggtggagc      960

ccctgcaccc ccttcacgga ggacccacct gcttccctgg aagtcctctc agagcgctgc     1020

tgggggacga tgcaggcagt ggagccgggg acagatgatg agggccccct gctggagcca     1080

gtgggcagtg agcatgccca ggatacctat ctggtgctgg acaaatggtt gctgccccgg     1140

aacccgccca gtgaggacct cccagggcct ggtggcagtg tggacatagt ggccatggat     1200

gaaggctcag aagcatcctc ctgctcatct gctttggcct cgaagcccag cccagaggga     1260

gcctctgctg ccagctttga gtacactatc ctggacccca gctcccagct cttgcgtcca     1320

ggatcaggcg agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc     1380

ccaatggcct taccagtgac cgccttgctc ctgccgctgg ccttgctgct ccacgccgcc     1440

aggccggaca tccagatgac acagactaca tcctccctgt ctgcctctct gggagacaga     1500

gtcaccatca gttgcagggc aagtcaggac attagtaaat atttaaattg gtatcagcag     1560

aaaccagatg gaactgttaa actcctgatc taccatacat caagattaca ctcaggagtc     1620

ccatcaaggt tcagtggcag tgggtctgga acagattatt ctctcaccat tagcaacctg     1680

gagcaagaag atattgccac ttacttttgc caacagggta atacgcttcc gtacacgttc     1740

ggagggggga ccaagctgga gatcacaggt ggcggtggct cgggcggtgg tgggtcgggt     1800

ggcggcggat ctgaggtgaa actgcaggag tcaggacctg gcctggtggc gccctcacag     1860

agcctgtccg tcacatgcac tgtctcaggg gtctcattac ccgactatgg tgtaagctgg     1920

attcgccagc ctccacgaaa gggtctggag tggctgggag taatatgggg tagtgaaacc     1980

acatactata attcagctct caaatccaga ctgaccatca tcaaggacaa ctccaagagc     2040

caagttttct taaaaatgaa cagtctgcaa actgatgaca cagccattta ctactgtgcc     2100

aaacattatt actacggtgg tagctatgct atggactact ggggccaagg aacctcagtc     2160

accgtctcct caaccacgac gccagcgccg cgaccaccaa caccggcgcc caccatcgcg     2220

tcgcagcccc tgtccctgcg cccagaggcg tgccggccag cggcgggggg cgcagtgcac     2280

acgagggggc tggacttcgc ctgtgatatc tacatctggg cgcccttggc cgggacttgt     2340

ggggtccttc tcctgtcact ggttatcacc ctttactgca aacggggcag aaagaaactc     2400

ctgtatatat tcaaacaacc atttatgaga ccagtacaaa ctactcaaga ggaagatggc     2460

tgtagctgcc gatttccaga agaagaagaa ggaggatgtg aactgagagt gaagttcagc     2520

aggagcgcag acgcccccgc gtaccagcag ggccagaacc agctctataa cgagctcaat     2580

ctaggacgaa gagaggagta cgatgttttg gacaagagac gtggccggga ccctgagatg     2640

gggggaaagc cgagaaggaa gaaccctcag gaaggcctgt acaatgaact gcagaaagat     2700

aagatggcgg aggcctacag tgagattggg atgaaaggcg agcgccggag gggcaagggg     2760

cacgatggcc tttaccaggg tctcagtaca gccaccaagg acacctacga cgcccttcac     2820

atgcaggccc tgccccctcg ctaa                                            2844


<210> 12
<211> 945
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      EpoRm-2A-CAR amino acid sequence

<400> 12
Met Asp His Leu Gly Ala Ser Leu Trp Pro Gln Val Gly Ser Leu Cys 
1               5                   10                  15      


Leu Leu Leu Ala Gly Ala Ala Trp Ala Pro Pro Pro Asn Leu Pro Asp 
            20                  25                  30          


Pro Lys Phe Glu Ser Lys Ala Ala Leu Leu Ala Ala Arg Gly Pro Glu 
        35                  40                  45              


Glu Leu Leu Cys Phe Thr Glu Arg Leu Glu Asp Leu Val Cys Phe Trp 
    50                  55                  60                  


Glu Glu Ala Ala Ser Ala Gly Val Gly Pro Gly Asn Tyr Ser Phe Ser 
65                  70                  75                  80  


Tyr Gln Leu Glu Asp Glu Pro Trp Lys Leu Cys Arg Leu His Gln Ala 
                85                  90                  95      


Pro Thr Ala Arg Gly Ala Val Arg Phe Trp Cys Ser Leu Pro Thr Ala 
            100                 105                 110         


Asp Thr Ser Ser Phe Val Pro Leu Glu Leu Arg Val Thr Ala Ala Ser 
        115                 120                 125             


Gly Ala Pro Arg Tyr His Arg Val Ile His Ile Asn Glu Val Val Leu 
    130                 135                 140                 


Leu Asp Ala Pro Val Gly Leu Val Ala Arg Leu Ala Asp Glu Ser Gly 
145                 150                 155                 160 


His Val Val Leu Arg Trp Leu Pro Pro Pro Glu Thr Pro Met Thr Ser 
                165                 170                 175     


His Ile Arg Tyr Glu Val Asp Val Ser Ala Gly Asn Gly Ala Gly Ser 
            180                 185                 190         


Val Gln Arg Val Glu Ile Leu Glu Gly Arg Thr Glu Cys Val Leu Ser 
        195                 200                 205             


Asn Leu Arg Gly Arg Thr Arg Tyr Thr Phe Ala Val Arg Ala Arg Met 
    210                 215                 220                 


Ala Glu Pro Ser Phe Gly Gly Phe Trp Ser Ala Trp Ser Glu Pro Val 
225                 230                 235                 240 


Ser Leu Leu Thr Pro Ser Asp Leu Asp Pro Leu Ile Leu Thr Leu Ser 
                245                 250                 255     


Leu Ile Leu Val Val Ile Leu Val Leu Leu Thr Val Leu Ala Leu Leu 
            260                 265                 270         


Ser His Arg Arg Ala Leu Lys Gln Lys Ile Trp Pro Gly Ile Pro Ser 
        275                 280                 285             


Pro Glu Ser Glu Phe Glu Gly Leu Phe Thr Thr His Lys Gly Asn Phe 
    290                 295                 300                 


Gln Leu Trp Leu Tyr Gln Asn Asp Gly Cys Leu Trp Trp Ser Pro Cys 
305                 310                 315                 320 


Thr Pro Phe Thr Glu Asp Pro Pro Ala Ser Leu Glu Val Leu Ser Glu 
                325                 330                 335     


Arg Cys Trp Gly Thr Met Gln Ala Val Glu Pro Gly Thr Asp Asp Glu 
            340                 345                 350         


Gly Pro Leu Leu Glu Pro Val Gly Ser Glu His Ala Gln Asp Thr Tyr 
        355                 360                 365             


Leu Val Leu Asp Lys Trp Leu Leu Pro Arg Asn Pro Pro Ser Glu Asp 
    370                 375                 380                 


Leu Pro Gly Pro Gly Gly Ser Val Asp Ile Val Ala Met Asp Glu Gly 
385                 390                 395                 400 


Ser Glu Ala Ser Ser Cys Ser Ser Ala Leu Ala Ser Lys Pro Ser Pro 
                405                 410                 415     


Glu Gly Ala Ser Ala Ala Ser Phe Glu Tyr Thr Ile Leu Asp Pro Ser 
            420                 425                 430         


Ser Gln Leu Leu Arg Pro Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu 
        435                 440                 445             


Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala Leu Pro Val 
    450                 455                 460                 


Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu His Ala Ala Arg Pro 
465                 470                 475                 480 


Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
                485                 490                 495     


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            500                 505                 510         


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        515                 520                 525             


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    530                 535                 540                 


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
545                 550                 555                 560 


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                565                 570                 575     


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            580                 585                 590         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        595                 600                 605             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    610                 615                 620                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
625                 630                 635                 640 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                645                 650                 655     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            660                 665                 670         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        675                 680                 685             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    690                 695                 700                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
705                 710                 715                 720 


Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
                725                 730                 735     


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
            740                 745                 750         


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        755                 760                 765             


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
    770                 775                 780                 


Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
785                 790                 795                 800 


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
                805                 810                 815     


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
            820                 825                 830         


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
        835                 840                 845             


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
    850                 855                 860                 


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
865                 870                 875                 880 


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
                885                 890                 895     


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
            900                 905                 910         


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
        915                 920                 925             


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
    930                 935                 940                 


Arg 
945 


<210> 13
<211> 66
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      P2A cDNA sequence

<400> 13
ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct       60

ggacct                                                                  66


<210> 14
<211> 22
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      P2A amino acid sequence

<400> 14
Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210> 15
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      T2A cDNA sequence

<400> 15
ggaagcggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctgga       60

cct                                                                     63


<210> 16
<211> 21
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      T2A amino acid sequence

<400> 16
Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210> 17
<211> 69
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      E2A cDNA sequence

<400> 17
ggaagcggac agtgtactaa ttatgctctc ttgaaattgg ctggagatgt tgagagcaac       60

cctggacct                                                               69


<210> 18
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      E2A amino acid sequence

<400> 18
Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210> 19
<211> 75
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      F2A cDNA sequence

<400> 19
ggaagcggag tgaaacagac tttgaatttt gaccttctca agttggcggg agacgtggag       60

tccaaccctg gacct                                                        75


<210> 20
<211> 25
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      F2A amino acid sequence

<400> 20
Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210> 21
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      CD8-alpha signal peptide cDNA sequence

<400> 21
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccg                                                                     63


<210> 22
<211> 21
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      CD8-alpha signal peptide amino acid sequence

<400> 22
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210> 23
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 23
Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210> 24
<211> 500
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MISC_FEATURE
<222> (1)..(500)
<223> This sequence may encompass 1-100 "Gly Gly Gly Gly Ser"
      repeating units

<400> 24
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    50                  55                  60                  


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
65                  70                  75                  80  


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                85                  90                  95      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                165                 170                 175     


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            180                 185                 190         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        195                 200                 205             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    210                 215                 220                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
225                 230                 235                 240 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                245                 250                 255     


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            260                 265                 270         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        275                 280                 285             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    290                 295                 300                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
305                 310                 315                 320 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                325                 330                 335     


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            340                 345                 350         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        355                 360                 365             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    370                 375                 380                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
385                 390                 395                 400 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                405                 410                 415     


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            420                 425                 430         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        435                 440                 445             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    450                 455                 460                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
465                 470                 475                 480 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
                485                 490                 495     


Gly Gly Gly Ser 
            500 


<210> 25
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 25
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210> 26
<211> 100
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MISC_FEATURE
<222> (1)..(100)
<223> This sequence may encompass 2-100 residues

<400> 26
Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
1               5                   10                  15      


Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
            20                  25                  30          


Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
        35                  40                  45              


Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
    50                  55                  60                  


Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
65                  70                  75                  80  


Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
                85                  90                  95      


Gly Gly Gly Gly 
            100 


<210> 27
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 27
Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15  


