               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> PROCESS FOR THE PRODUCTION OF ETHANOL

<130> 32898-WO-PCT

<160> 7

<170> BiSSAP 1.3.6

<210> 1
<211> 561
<212> PRT
<213> Punctularia strigosozonata


<220> 
<223> Punctularia strigosozonata glucoamylase

<400> 1
Ser Ser Lys Ser Val Ser Ala Tyr Ile Ser Ser Glu Ser Pro Ile Ala 
1               5                   10                  15      
His Ser Lys Leu Leu Asp Asn Ile Gly Pro Asp Gly Ala Lys Ala Pro 
            20                  25                  30          
Gly Ala Phe Pro Gly Val Val Val Ala Ser Pro Ser Thr Asp Asn Pro 
        35                  40                  45              
Asn Tyr Tyr Tyr Ser Trp Ile Arg Asp Ser Ser Leu Val Phe Lys Thr 
    50                  55                  60                  
Leu Ile Asp Asp Tyr Val Asn Gly Lys Asn Thr Ser Lys Ser Leu Arg 
65                  70                  75                  80  
Ser Leu Ile Asp Asp Phe Val Thr Ala Ser Ser Val Phe Gln Gln Thr 
                85                  90                  95      
Pro Asn Pro Ser Gly Asn Val Ser Thr Gly Gly Leu Gly Glu Pro Lys 
            100                 105                 110         
Phe Tyr Val Asn Glu Thr Ala Phe Leu Asp Ser Trp Gly Arg Pro Gln 
        115                 120                 125             
Arg Asp Gly Pro Ala Leu Arg Ser Thr Ala Leu Ile Thr Tyr Ala Asn 
    130                 135                 140                 
Tyr Leu Leu Asp Asn Asp Asn Thr Thr Trp Val Lys Asp Thr Leu Trp 
145                 150                 155                 160 
Pro Ile Ile Glu Leu Asp Val Asn Tyr Val Ser Asp Phe Trp Asn Tyr 
                165                 170                 175     
Thr Thr Phe Asp Leu Trp Glu Glu Val Ala Ser Ser Ser Phe Phe Thr 
            180                 185                 190         
Thr Ala Val Gln His Arg Ala Leu Arg Gln Ala Ser Lys Leu Ala Lys 
        195                 200                 205             
Thr Leu Asp Lys Thr Asp Asn Ile Asp Ser Trp Asn Thr Gln Ala Asp 
    210                 215                 220                 
Asn Val Leu Cys Phe Leu Gln Ser Tyr Trp Asn Gly Ser Ala Ile Ile 
225                 230                 235                 240 
Ala Asn Thr Gly Gly Gly Arg Ser Gly Ile Asp Ala Asn Thr Val Leu 
                245                 250                 255     
Ala Ser Ile His Thr Phe Asp Ser Ser Ala Gly Cys Asp Ala Thr Thr 
            260                 265                 270         
Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr Val 
        275                 280                 285             
Asp Ala Phe Arg Ser Ile Tyr Glu Ile Asn Ser Gly Ile Asp Pro Asn 
    290                 295                 300                 
Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Val Phe Tyr Asp Gly 
305                 310                 315                 320 
Asn Pro Trp Tyr Leu Ala Thr Ala Ala Val Ala Glu Gln Leu Tyr Asp 
                325                 330                 335     
Ala Leu Tyr Val Trp Asn Thr Thr Gly Ser Leu Glu Ile Thr Asp Ile 
            340                 345                 350         
Ser Leu Pro Phe Phe Gln Gln Phe Asp Ser Asp Val Lys Thr Gly Thr 
        355                 360                 365             
Tyr Ser Asp Asp Asp Thr Phe Asp Ser Leu Ile Ser Ser Ile Gln Ser 
    370                 375                 380                 
Phe Ala Asp Gly Phe Leu Glu Ile His Ala Lys Tyr Thr Pro Asp Asp 
385                 390                 395                 400 
Gly Ala Leu Ser Glu Glu Phe Ser Lys Thr Asp Gly Ser Gln Thr Ser 
                405                 410                 415     
Ala Ala Asp Leu Thr Trp Ser Tyr Ala Ala Ala Leu Thr Ala Phe Asp 
            420                 425                 430         
Ala Arg Ser Arg Asp Ala Ala Val Lys Trp Gly Ala Lys Gly Leu Gln 
        435                 440                 445             
Val Pro Asp Gly Thr Cys Lys Thr Asn Glu Gly Gly Asp Asp Gly Leu 
    450                 455                 460                 
Gly Val Pro Val Thr Phe Leu Val Lys Asp Ala Glu Thr Val Glu Gly 
465                 470                 475                 480 
Gln Ser Val Tyr Ile Thr Gly Ser Ile Ala Thr Leu Lys Ser Trp Ser 
                485                 490                 495     
Pro Asp Asp Ala Leu Leu Met Ser Pro Ser Asp Tyr Pro Thr Trp Thr 
            500                 505                 510         
Leu Thr Val Asn Leu Ser Ala Ser Glu Ser Val Gln Tyr Lys Tyr Ile 
        515                 520                 525             
Lys Lys Asp Thr Ala Gly Thr Val Ile Trp Glu Ser Asp Pro Asn Asn 
    530                 535                 540                 
Ser Leu Leu Val Pro Ser Gly Gly Ser Val Thr Thr Asp Asp Thr Trp 
545                 550                 555                 560 
Arg 
    

<210> 2
<211> 578
<212> PRT
<213> Punctularia strigosozonata


<220> 
<223> Punctularia strigosozonata glucoamylase with native signal
      sequence FGA09

<400> 2
Met Leu Ser Ser Leu Ile Val Ser Gly Leu Leu Ala Ser Gly Val Cys 
1               5                   10                  15      
Ala Ser Ser Lys Ser Val Ser Ala Tyr Ile Ser Ser Glu Ser Pro Ile 
            20                  25                  30          
Ala His Ser Lys Leu Leu Asp Asn Ile Gly Pro Asp Gly Ala Lys Ala 
        35                  40                  45              
Pro Gly Ala Phe Pro Gly Val Val Val Ala Ser Pro Ser Thr Asp Asn 
    50                  55                  60                  
Pro Asn Tyr Tyr Tyr Ser Trp Ile Arg Asp Ser Ser Leu Val Phe Lys 
65                  70                  75                  80  
Thr Leu Ile Asp Asp Tyr Val Asn Gly Lys Asn Thr Ser Lys Ser Leu 
                85                  90                  95      
Arg Ser Leu Ile Asp Asp Phe Val Thr Ala Ser Ser Val Phe Gln Gln 
            100                 105                 110         
Thr Pro Asn Pro Ser Gly Asn Val Ser Thr Gly Gly Leu Gly Glu Pro 
        115                 120                 125             
Lys Phe Tyr Val Asn Glu Thr Ala Phe Leu Asp Ser Trp Gly Arg Pro 
    130                 135                 140                 
Gln Arg Asp Gly Pro Ala Leu Arg Ser Thr Ala Leu Ile Thr Tyr Ala 
145                 150                 155                 160 
Asn Tyr Leu Leu Asp Asn Asp Asn Thr Thr Trp Val Lys Asp Thr Leu 
                165                 170                 175     
Trp Pro Ile Ile Glu Leu Asp Val Asn Tyr Val Ser Asp Phe Trp Asn 
            180                 185                 190         
Tyr Thr Thr Phe Asp Leu Trp Glu Glu Val Ala Ser Ser Ser Phe Phe 
        195                 200                 205             
Thr Thr Ala Val Gln His Arg Ala Leu Arg Gln Ala Ser Lys Leu Ala 
    210                 215                 220                 
Lys Thr Leu Asp Lys Thr Asp Asn Ile Asp Ser Trp Asn Thr Gln Ala 
225                 230                 235                 240 
Asp Asn Val Leu Cys Phe Leu Gln Ser Tyr Trp Asn Gly Ser Ala Ile 
                245                 250                 255     
Ile Ala Asn Thr Gly Gly Gly Arg Ser Gly Ile Asp Ala Asn Thr Val 
            260                 265                 270         
Leu Ala Ser Ile His Thr Phe Asp Ser Ser Ala Gly Cys Asp Ala Thr 
        275                 280                 285             
Thr Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr 
    290                 295                 300                 
Val Asp Ala Phe Arg Ser Ile Tyr Glu Ile Asn Ser Gly Ile Asp Pro 
305                 310                 315                 320 
Asn Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Val Phe Tyr Asp 
                325                 330                 335     
Gly Asn Pro Trp Tyr Leu Ala Thr Ala Ala Val Ala Glu Gln Leu Tyr 
            340                 345                 350         
Asp Ala Leu Tyr Val Trp Asn Thr Thr Gly Ser Leu Glu Ile Thr Asp 
        355                 360                 365             
Ile Ser Leu Pro Phe Phe Gln Gln Phe Asp Ser Asp Val Lys Thr Gly 
    370                 375                 380                 
Thr Tyr Ser Asp Asp Asp Thr Phe Asp Ser Leu Ile Ser Ser Ile Gln 
385                 390                 395                 400 
Ser Phe Ala Asp Gly Phe Leu Glu Ile His Ala Lys Tyr Thr Pro Asp 
                405                 410                 415     
Asp Gly Ala Leu Ser Glu Glu Phe Ser Lys Thr Asp Gly Ser Gln Thr 
            420                 425                 430         
Ser Ala Ala Asp Leu Thr Trp Ser Tyr Ala Ala Ala Leu Thr Ala Phe 
        435                 440                 445             
Asp Ala Arg Ser Arg Asp Ala Ala Val Lys Trp Gly Ala Lys Gly Leu 
    450                 455                 460                 
Gln Val Pro Asp Gly Thr Cys Lys Thr Asn Glu Gly Gly Asp Asp Gly 
465                 470                 475                 480 
Leu Gly Val Pro Val Thr Phe Leu Val Lys Asp Ala Glu Thr Val Glu 
                485                 490                 495     
Gly Gln Ser Val Tyr Ile Thr Gly Ser Ile Ala Thr Leu Lys Ser Trp 
            500                 505                 510         
Ser Pro Asp Asp Ala Leu Leu Met Ser Pro Ser Asp Tyr Pro Thr Trp 
        515                 520                 525             
Thr Leu Thr Val Asn Leu Ser Ala Ser Glu Ser Val Gln Tyr Lys Tyr 
    530                 535                 540                 
Ile Lys Lys Asp Thr Ala Gly Thr Val Ile Trp Glu Ser Asp Pro Asn 
545                 550                 555                 560 
Asn Ser Leu Leu Val Pro Ser Gly Gly Ser Val Thr Thr Asp Asp Thr 
                565                 570                 575     
Trp Arg 
        

<210> 3
<211> 17
<212> PRT
<213> Punctularia strigosozonata


<220> 
<223> Punctularia strigosozonata glucoamylase signal sequence

<400> 3
Met Leu Ser Ser Leu Ile Val Ser Gly Leu Leu Ala Ser Gly Val Cys 
1               5                   10                  15      
Ala 
    

<210> 4
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> connector sequence

<400> 4
tcctgtacgc actttgtccc acaaattccc gattccgcaa tttgttcgcc                50


<210> 5
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> connector sequence

<400> 5
gaacaggata tttcgatctt ggatacgtac tcgcttgtgt ctggtttcgt                50


<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> integration target sequence

<400> 6
agaaaactct tagcttttcc                                                20


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> integration target sequence

<400> 7
caatatggta tgccgagtct                                                20


