                         SEQUENCE LISTING

<110>  Liu, Shirong
       Weiner, Howard
 
<120>  The Microbiome as a Target of MicroRNAs for the Treatment of Disease

<130>  29618-0212WO1

<150>  US 62/722,136
<151>  2018-08-23

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  70
<212>  RNA
<213>  Homo sapiens

<400>  1
guuguuguaa acauccccga cuggaagcug uaagacacag cuaagcuuuc agucagaugu       60

uugcugcuac                                                              70


<210>  2
<211>  22
<212>  RNA
<213>  Homo sapiens

<400>  2
uguaaacauc cccgacugga ag                                                22


<210>  3
<211>  67
<212>  RNA
<213>  Homo sapiens

<400>  3
uggagcugug ugcagggcca gcgcggagcc cgagcagccg cggugaagcg ccugugcucu       60

gccgaga                                                                 67


<210>  4
<211>  24
<212>  RNA
<213>  Homo sapiens

<400>  4
ugaagcgccu gugcucugcc gaga                                              24


<210>  5
<211>  73
<212>  RNA
<213>  Homo sapiens

<400>  5
uguauccuug aauggauuuu uggagcagga guggacaccu gacccaaagg aaaucaaucc       60

auaggcuagc aau                                                          73


<210>  6
<211>  19
<212>  RNA
<213>  Homo sapiens

<400>  6
aauggauuuu uggagcagg                                                    19


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  7
tcctacggga ggcagcagt                                                    19


<210>  8
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  8
ggactaccag ggtatctaat cctgtt                                            26


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  9
cgtattaccg cggctgctgg cac                                               23


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  10
cggtggagta tgtggcttaa t                                                 21


<210>  11
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  11
ccatgcagca cctgtgtaa                                                    19


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  12
cgcctccgaa gagtcgcatg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  13
aggccttcgg gttgtaaagt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  14
cggggatttc acatctgact                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  15
cagaagaagc accggctaac                                                   20


<210>  16
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  scrambled control sequence

<400>  16
guggaugaac cgcaacuacc au                                                22


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  17
ccatttacgg cagaaacagc                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  18
gccagggaga gggtttttac                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  19
cgtgaaggaa atagccctga                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  20
tgaaagggag ggttcatctg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  21
atccacacgg gcagagtaat                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  22
tttatagaaa tgcgggtggc                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  23
caacatggaa acctccatcc                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  24
gaccagttcc tgggtgacat                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  25
agacttttgt ggacatgggg                                                   20


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  26
cggtggagta tgtggcttaa t                                                 21


<210>  27
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  27
ccatgcagca cctgtgtaa                                                    19


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  28
cgcctccgaa gagtcgcatg                                                   20


<210>  29
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  29
gctctagagc atgaaatttg tcgccaaaat cctg                                   34


<210>  30
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  30
ggggtacccc ttattcaatg ctcttgagca cttc                                   34


<210>  31
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  31
gctctagagc atgaaatttg tcgcctaata atcctgacca tcgccgc                     47


<210>  32
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  32
ggggtacccc ttattcaatg ctcttgagca cttc                                   34


<210>  33
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  33
gctctagagc atgaatgtta tgtcgaaacg tttttttgcc                             40


<210>  34
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  34
ggggtacccc atttaccggg tcagcatgcc gttggctat                              39


<210>  35
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  35
cccagtcacg acgttgtaaa acg                                               23


<210>  36
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR Primer

<400>  36
agcggataac aatttcacac agg                                               23


<210>  37
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-30d-5p binding sequence

<400>  37
cggcuccaac aucaccgacu ggggcgau                                          28


<210>  38
<211>  33
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-30d-5p binding sequence

<400>  38
aucguuuauu ucggggaugu ucagaaucug ugg                                    33


<210>  39
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-30d-5p binding sequence

<400>  39
cccugaagaa agaccugaac auccccgucu ggauccacac                             40


<210>  40
<211>  320
<212>  PRT
<213>  Akkermansia muciniphila

<400>  40

Met Lys Phe Val Ala Lys Ile Leu Thr Ile Ala Ala Ala Leu Ser Ser 
1               5                   10                  15      


Leu Ser Met Gly Ala Asn Gln Val Thr Val Asp Ser Ser Ile Lys Pro 
            20                  25                  30          


Tyr Ala Pro Thr Ser Gly Val Ser Gly Asn Leu Asn Ala Val Gly Ser 
        35                  40                  45              


Asp Thr Leu Asn Asn Leu Met Thr Leu Trp Ala Glu Gly Phe Asn Lys 
    50                  55                  60                  


Lys Tyr Pro Ser Val Lys Val Gly Val Glu Gly Lys Gly Ser Ser Thr 
65                  70                  75                  80  


Ala Pro Pro Ala Leu Thr Ala Gly Thr Ala Gln Leu Ala Pro Met Ser 
                85                  90                  95      


Arg Gln Met Lys Arg Glu Glu Ile Ala Ala Phe Glu Ala Lys Tyr Gly 
            100                 105                 110         


Tyr Lys Pro Thr Glu Ile Lys Val Ala Leu Asp Ala Val Ala Phe Phe 
        115                 120                 125             


Val Asn Lys Asn Asn Pro Ile Gln Ala Leu Thr Leu Thr Gln Ile Asp 
    130                 135                 140                 


Ser Ile Phe Ser Ser Thr Phe Lys Arg Gly Gly Ser Asn Ile Thr Asp 
145                 150                 155                 160 


Trp Gly Asp Ala Gly Val Pro Ser Met Lys Gly Lys Ala Ile Ser Ile 
                165                 170                 175     


Tyr Gly Arg Asn Ser Ala Ser Gly Thr Asn Gly Phe Val Lys Glu Ile 
            180                 185                 190         


Ala Leu Lys Lys Gly Asp Tyr Lys Asn Ser Val Lys Glu Gln Pro Gly 
        195                 200                 205             


Ser Ser Ala Val Val Gln Gly Ile Ser Ser Asp Glu Gln Gly Ile Gly 
    210                 215                 220                 


Tyr Ser Gly Ile Gly Tyr Val Thr Ser Gly Val Lys Thr Leu Ser Leu 
225                 230                 235                 240 


Ala Glu Lys Ser Gly Lys Ala Ala Val Gln Pro Ser Tyr Asp Asn Cys 
                245                 250                 255     


Ile Asn Gly Thr Tyr Pro Leu Ser Arg Tyr Leu Leu Ile Tyr Val Asn 
            260                 265                 270         


Lys Lys Pro Gly Glu Pro Leu Asp Thr Leu Thr Arg Glu Phe Ile Lys 
        275                 280                 285             


Phe Ile Val Ser Lys Asp Gly Gln Glu Ile Val Thr Lys Asp Gly Tyr 
    290                 295                 300                 


Tyr Pro Ile Pro Ala Lys Val Ser Ala Glu Val Leu Lys Ser Ile Glu 
305                 310                 315                 320 


<210>  41
<211>  674
<212>  PRT
<213>  Ktedonobacter racemifer

<400>  41

Met Gln Gln Tyr Phe Pro Asn Lys Ile Leu Tyr Gly Gly Asp Tyr Asn 
1               5                   10                  15      


Pro Glu Gln Trp Ser Glu Glu Thr Trp Arg Glu Asp Met Arg Leu Met 
            20                  25                  30          


Lys Leu Ala His Val Asn Met Val Ser Ile Asn Ile Phe Ser Trp Thr 
        35                  40                  45              


Leu Leu Glu Pro Glu Pro His Lys Tyr His Phe Glu Gln Leu Asp Arg 
    50                  55                  60                  


Ile Met Asp Met Leu Ala Glu His Gly Ile Tyr Ala Asp Leu Ala Thr 
65                  70                  75                  80  


Ala Thr Ala Ser Pro Pro Thr Trp Met Ser Arg Leu Tyr Pro Ser Met 
                85                  90                  95      


Leu Pro Val Thr Gln Gln Gly Val Arg Met Ser His Gly Ser Arg Gln 
            100                 105                 110         


His Tyr Cys Pro Asn Ser Pro Asp Tyr Arg Arg Lys Ala Gly Ala Leu 
        115                 120                 125             


Val Glu Gln Ile Ala Thr Arg Tyr Ala Lys His Pro Ala Leu Lys Met 
    130                 135                 140                 


Trp His Leu Asn Asn Glu Tyr Gly Cys His Thr Gly Val Cys Tyr Cys 
145                 150                 155                 160 


Glu Asn Cys Ala Ala Ala Phe Arg Lys Trp Leu Gln Glu Arg Tyr Gln 
                165                 170                 175     


Thr Leu Asp Lys Val Asn Gln Ala Trp Gly Thr Thr Phe Trp Ser Gln 
            180                 185                 190         


His Tyr Tyr Glu Trp Glu Asp Val Leu Pro Pro Arg Ala Thr Pro Ala 
        195                 200                 205             


Gln Thr Asn Pro Thr Gln Thr Leu Asp Tyr Trp Arg Phe Met Asn Asp 
    210                 215                 220                 


Ser Leu Arg Gly Cys Tyr Glu Leu Glu Glu Glu Ile Leu Arg Arg Thr 
225                 230                 235                 240 


Thr Pro Ser Ile Pro Leu Thr Thr Asn Leu Met Val Ala Phe Lys Pro 
                245                 250                 255     


Val Asp Val Phe Asp Trp Ala Lys His Met Asp Ile Val Ser Phe Asp 
            260                 265                 270         


Met Tyr Pro Thr Pro His Asp Gly Ala Ala Gln Val Ala Met Pro His 
        275                 280                 285             


Asp Ile Met Arg Gly Ala Lys Gly Gly Gln Pro His Ile Val Met Glu 
    290                 295                 300                 


Met Ser Pro Ser Gln Val Asn Trp Gln Pro Gln Thr Pro His Lys Arg 
305                 310                 315                 320 


Pro Gly Gln Leu Arg Met His Ile Met Gln Ser Ile Ala Arg Gly Ala 
                325                 330                 335     


Asn Gly Ala Leu Phe Phe Gln Trp Arg Gln Ser Met Ala Gly Ala Glu 
            340                 345                 350         


Lys Tyr His Ser Ala Val Val Ser His Glu Gly Ser Glu Gln Asn Arg 
        355                 360                 365             


Ile Phe Lys Gln Val Ala Gln Ala Gly Ala Glu Leu Ala Lys Leu Ala 
    370                 375                 380                 


Ser Gln Val Ala Asn Thr Arg Ile His Ala Lys Val Ala Leu Leu Met 
385                 390                 395                 400 


Asp Trp Gln Ser Trp Trp Ser Ser Glu Tyr Gln Pro Gly Pro Ser Asp 
                405                 410                 415     


Gln Leu Arg Tyr Tyr Glu Gln Ile Leu Thr Tyr Tyr Lys Ala Leu Tyr 
            420                 425                 430         


Ala Arg Asn Ile Ala Val Asp Ile Val Ser Pro Glu Ala Glu Leu Gly 
        435                 440                 445             


Glu Asn Leu Gly Lys Tyr Lys Leu Val Ile Ala Pro Leu Leu His Met 
    450                 455                 460                 


Ile Met Pro Gly Val Glu Gln Lys Leu Lys Gly Phe Val Glu Gln Gly 
465                 470                 475                 480 


Gly Thr Phe Leu Thr Thr Phe Phe Ser Gly Ile Val Asp Glu His Glu 
                485                 490                 495     


His Val Ile Pro Gly Gly Tyr Pro Gly Ala Leu Arg Glu Leu Leu Gly 
            500                 505                 510         


Ile Tyr Val Glu Glu Phe Asp Pro Leu Thr Pro Gln Met Ser Asn Glu 
        515                 520                 525             


Val Val Ile Glu Glu Gly Glu Leu Arg Gly Arg Tyr Ala Ala Thr Arg 
    530                 535                 540                 


Trp Gly Glu Leu Val His Leu Lys Gly Ala Gln Ala Leu Ala Arg Phe 
545                 550                 555                 560 


Gly Gln Asp Tyr Tyr Ala Gln Gln Pro Ala Ile Thr Glu His Ser Tyr 
                565                 570                 575     


Gly Gln Gly Lys Ala Tyr Tyr Val Ala Thr His Pro Glu Gln Arg Leu 
            580                 585                 590         


Val Asp Ala Leu Ile Lys Gln Ile Cys Ala Gln Ala Gly Val Glu Pro 
        595                 600                 605             


Val Leu Ser Thr Pro Glu Gly Val Glu Val Thr Leu Arg Glu Gly Glu 
    610                 615                 620                 


His Gly Glu Lys Phe Tyr Phe Val Leu Asn Gln Ser Lys Glu Arg Gln 
625                 630                 635                 640 


Gln Ile Thr Leu Ser Glu Gly Ser Tyr Thr Ser Leu Leu Asp Glu Gly 
                645                 650                 655     


Lys Val Gln Glu Thr Ile Thr Ile Glu Pro Phe Asp Val Leu Val Leu 
            660                 665                 670         


Lys Gly 
        


