                         SEQUENCE LISTING

<110>  The Broad Institute, Inc.
       Brigham and Women's Hospital, Inc.
       Choudhary, Amit
       Manna, Debasish
       Maji, Basudeb
       Gangopadhyay, Soumyashree Ashok
 
<120>  Methods and Compositions for Optochemical Control of CRISPR-CAS9

<130>  BROD-3870

<150>  62/765,346
<151>  2018-08-20

<150>  62/773,997
<151>  2018-11-30

<160>  116   

<170>  PatentIn version 3.5

<210>  1
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  1
acttgtttaa gt                                                           12


<210>  2
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n = any nucleotides that are complementary to nnnn at position 8 
       to 12

<220>
<221>  misc_feature
<222>  (8)..(12)
<223>  n = any nucleotides that are complementary to nnnn at position 1 
       to 4

<400>  2
nnnngtttnn nn                                                           12


<210>  3
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  3
ggcaccgagt cggtgc                                                       16


<210>  4
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  n = any nucleotide that is complementary to nnnnnnn at position 
       11 to 17

<220>
<221>  misc_feature
<222>  (11)..(17)
<223>  n = any nucleotide that is complementary to nnnnnnn at position 1
       to 7

<400>  4
nnnnnnnagt nnnnnnn                                                      17


<210>  5
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (2)..(5)
<223>  y = t/u or c

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n = part of the bulge of the duplex

<220>
<221>  misc_feature
<222>  (9)..(12)
<223>  n = any nucleotide that is complementary to nnnn at position 17 
       to 20

<220>
<221>  misc_feature
<222>  (13)..(16)
<223>  n = any nucleotide

<220>
<221>  misc_feature
<222>  (17)..(20)
<223>  n = any nucleotide that is complementary to nnnn at position 9 to
       12

<220>
<221>  misc_feature
<222>  (21)..(23)
<223>  part of the bulge in the duplex

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n = any nucleotide

<220>
<221>  misc_feature
<222>  (26)..(29)
<223>  r = g or a

<400>  5
gyyyyagnnn nnnnnnnnnn aanuurrrru                                        30


<210>  6
<211>  7
<212>  PRT
<213>  SV40 virus

<400>  6

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  7
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  7

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  8
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  8

Pro Ala Ala Lys Arg Val Lys Leu Asp 
1               5                   


<210>  9
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  9

Arg Gln Arg Arg Asn Glu Leu Lys Arg Ser Pro 
1               5                   10      


<210>  10
<211>  38
<212>  PRT
<213>  Homo sapiens

<400>  10

Asn Gln Ser Ser Asn Phe Gly Pro Met Lys Gly Gly Asn Phe Gly Gly 
1               5                   10                  15      


Arg Ser Ser Gly Pro Tyr Gly Gly Gly Gly Gln Tyr Phe Ala Lys Pro 
            20                  25                  30          


Arg Asn Gln Gly Gly Tyr 
        35              


<210>  11
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  11

Arg Met Arg Ile Glx Phe Lys Asn Lys Gly Lys Asp Thr Ala Glu Leu 
1               5                   10                  15      


Arg Arg Arg Arg Val Glu Val Ser Val Glu Leu Arg Lys Ala Lys Lys 
            20                  25                  30          


Asp Glu Gln Ile Leu Lys Arg Arg Asn Val 
        35                  40          


<210>  12
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  12

Val Ser Arg Lys Arg Pro Arg Pro 
1               5               


<210>  13
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  13

Pro Pro Lys Lys Ala Arg Glu Asp 
1               5               


<210>  14
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  14

Pro Gln Pro Lys Lys Lys Pro Leu 
1               5               


<210>  15
<211>  12
<212>  PRT
<213>  Mus musculus

<400>  15

Ser Ala Leu Ile Lys Lys Lys Lys Lys Met Ala Pro 
1               5                   10          


<210>  16
<211>  5
<212>  PRT
<213>  Influenza virus

<400>  16

Asp Arg Leu Arg Arg 
1               5   


<210>  17
<211>  7
<212>  PRT
<213>  Influenza virus

<400>  17

Pro Lys Gln Lys Lys Arg Lys 
1               5           


<210>  18
<211>  10
<212>  PRT
<213>  Hepatitis delta virus

<400>  18

Arg Lys Leu Lys Lys Lys Ile Lys Lys Leu 
1               5                   10  


<210>  19
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  19

Arg Glu Lys Lys Lys Phe Leu Lys Arg Arg 
1               5                   10  


<210>  20
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  20

Lys Arg Lys Gly Asp Glu Val Asp Gly Val Asp Glu Val Ala Lys Lys 
1               5                   10                  15      


Lys Ser Lys Lys 
            20  


<210>  21
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  21

Arg Lys Cys Leu Gln Ala Gly Met Asn Leu Glu Ala Arg Lys Thr Lys 
1               5                   10                  15      


Lys 
    


<210>  22
<211>  23
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (8)..(20)
<223>  n = a, g, c, or t

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n = any nucleotide

<400>  22
mmmmmmmmnn nnnnnnnnnn ngg                                               23


<210>  23
<211>  15
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  n = a, g, c, or t

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n = any nucleotide

<400>  23
nnnnnnnnnn nnngg                                                        15


<210>  24
<211>  23
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (10)..(20)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n = any nucleotide

<400>  24
mmmmmmmmmn nnnnnnnnnn ngg                                               23


<210>  25
<211>  14
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  N = any nucleotide

<400>  25
nnnnnnnnnn nngg                                                         14


<210>  26
<211>  27
<212>  DNA
<213>  Streptococcus thermophilus


<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (9)..(20)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  w = a or t

<400>  26
mmmmmmmmnn nnnnnnnnnn nnagaaw                                           27


<210>  27
<211>  19
<212>  DNA
<213>  Streptococcus thermophilus


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  w = a or t

<400>  27
nnnnnnnnnn nnnnagaaw                                                    19


<210>  28
<211>  27
<212>  DNA
<213>  Streptococcus thermophilus


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (11)..(20)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  n = any nucleotide

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  w = a or t

<400>  28
mmmmmmmmmn nnnnnnnnnn nnagaaw                                           27


<210>  29
<211>  18
<212>  DNA
<213>  Streptococcus thermophilus


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (12)..(13)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  w = a or t

<400>  29
nnnnnnnnnn nnnagaaw                                                     18


<210>  30
<211>  25
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (9)..(20)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  N = any nucleotide

<400>  30
mmmmmmmmnn nnnnnnnnnn nggng                                             25


<210>  31
<211>  17
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  N = any nucleotide

<400>  31
nnnnnnnnnn nnnggng                                                      17


<210>  32
<211>  25
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (10)..(20)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  N = any nucleotide

<400>  32
mmmmmmmmmn nnnnnnnnnn nggng                                             25


<210>  33
<211>  16
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  N = any nucleotide

<400>  33
nnnnnnnnnn nnggng                                                       16


<210>  34
<211>  137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n = any nucleotide

<400>  34
nnnnnnnnnn nnnnnnnnnn gtttttgtac tctcaagatt tagaaataaa tcttgcagaa       60

gctacaaaga taaggcttca tgccgaaatc aacaccctgt cattttatgg cagggtgttt      120

tcgttattta atttttt                                                     137


<210>  35
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n = any nucleotide

<400>  35
nnnnnnnnnn nnnnnnnnnn gtttttgtac tctcagaaat gcagaagcta caaagataag       60

gcttcatgcc gaaatcaaca ccctgtcatt ttatggcagg gtgttttcgt tatttaattt      120

ttt                                                                    123


<210>  36
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n = any nucleotide

<400>  36
nnnnnnnnnn nnnnnnnnnn gtttttgtac tctcagaaat gcagaagcta caaagataag       60

gcttcatgcc gaaatcaaca ccctgtcatt ttatggcagg gtgttttttt                 110


<210>  37
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n = any nucleotide

<400>  37
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  38
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n = any nucleotide

<400>  38
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt gttttttt                                          88


<210>  39
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n = any nucleotide

<400>  39
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcatt tttttt                                                       76


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  40
gagtccgagc agaagaagaa                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  41
gagtcctagc aggagaagaa                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  42
gagtctaagc agaagaagaa                                                   20


<210>  43
<211>  6
<212>  PRT
<213>  SV40 virus

<400>  43

Pro Lys Lys Lys Arg Lys 
1               5       


<210>  44
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  44

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  45
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  45

Pro Ala Ala Lys Arg Val Lys Leu Asp 
1               5                   


<210>  46
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  46

Arg Gln Arg Arg Asn Glu Leu Lys Arg Ser Pro 
1               5                   10      


<210>  47
<211>  38
<212>  PRT
<213>  Homo sapiens

<400>  47

Asn Gln Ser Ser Asn Phe Gly Pro Met Lys Gly Gly Asn Phe Gly Gly 
1               5                   10                  15      


Arg Ser Ser Gly Pro Tyr Gly Gly Gly Gly Gln Tyr Phe Ala Lys Pro 
            20                  25                  30          


Arg Asn Gln Gly Gly Tyr 
        35              


<210>  48
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  48

Arg Met Arg Ile Glx Phe Lys Asn Lys Gly Lys Asp Thr Ala Glu Leu 
1               5                   10                  15      


Arg Arg Arg Arg Val Glu Val Ser Val Glu Leu Arg Lys Ala Lys Lys 
            20                  25                  30          


Asp Glu Gln Ile Leu Lys Arg Arg Asn Val 
        35                  40          


<210>  49
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  49

Val Ser Arg Lys Arg Pro Arg Pro 
1               5               


<210>  50
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  50

Pro Pro Lys Lys Ala Arg Glu Asp 
1               5               


<210>  51
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  51

Pro Gln Pro Lys Lys Lys Pro Leu 
1               5               


<210>  52
<211>  12
<212>  PRT
<213>  Mus musculus

<400>  52

Ser Ala Leu Ile Lys Lys Lys Lys Lys Met Ala Pro 
1               5                   10          


<210>  53
<211>  5
<212>  PRT
<213>  Influenza virus

<400>  53

Asp Arg Leu Arg Arg 
1               5   


<210>  54
<211>  7
<212>  PRT
<213>  Influenza virus

<400>  54

Pro Lys Gln Lys Lys Arg Lys 
1               5           


<210>  55
<211>  10
<212>  PRT
<213>  Hepatitis virus delta

<400>  55

Arg Lys Leu Lys Lys Lys Ile Lys Lys Leu 
1               5                   10  


<210>  56
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  56

Arg Glu Lys Lys Lys Phe Leu Lys Arg Arg 
1               5                   10  


<210>  57
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  57

Lys Arg Lys Gly Asp Glu Val Asp Gly Val Asp Glu Val Ala Lys Lys 
1               5                   10                  15      


Lys Ser Lys Lys 
            20  


<210>  58
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  58

Arg Lys Cys Leu Gln Ala Gly Met Asn Leu Glu Ala Arg Lys Thr Lys 
1               5                   10                  15      


Lys 
    


<210>  59
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  59

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  60
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  60

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10          


<210>  61
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  61

Ala Glu Ala Ala Ala Lys Ala 
1               5           


<210>  62
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  62

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25                  30  


<210>  63
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  63

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        35                  40                  45  


<210>  64
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  64

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    50                  55                  60  


<210>  65
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  65

Gly Gly Gly Gly Ser 
1               5   


<210>  66
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  66

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  67
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  67

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  68
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  68

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25  


<210>  69
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  69

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser 
        35  


<210>  70
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  70

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser 
        35                  40  


<210>  71
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  71

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser 
    50  


<210>  72
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  72

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Gly Ser 
    50                  55  


<210>  73
<211>  414
<212>  DNA
<213>  Streptococcus pyogenes

<400>  73
ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac       60

cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct      120

gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag      180

aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag      240

agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac      300

gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc      360

aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgag            414


<210>  74
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  74
gagtccgagc agaagaagaa                                                   20


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  75
gagtcctagc aggagaagaa                                                   20


<210>  76
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  76
gagtctaagc agaagaagaa                                                   20


<210>  77
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  77

Phe Trp Tyr His Lys Met Ile Leu Val Ala Gly Cys 
1               5                   10          


<210>  78
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  78

Trp Tyr His Lys Arg Glu Asp Cys Ser Thr Asn Gln 
1               5                   10          


<210>  79
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  79

Val Cys Ala Gly Ser Pro Thr Asn Asp 
1               5                   


<210>  80
<211>  12
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  80
guuuuagagc ua                                                           12


<210>  81
<211>  7
<212>  PRT
<213>  SV40 virus

<400>  81

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  82
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  82

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  83
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  83

Pro Ala Ala Lys Arg Val Lys Leu Asp 
1               5                   


<210>  84
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  84

Arg Gln Arg Arg Asn Glu Leu Lys Arg Ser Pro 
1               5                   10      


<210>  85
<211>  38
<212>  PRT
<213>  Homo sapiens

<400>  85

Asn Gln Ser Ser Asn Phe Gly Pro Met Lys Gly Gly Asn Phe Gly Gly 
1               5                   10                  15      


Arg Ser Ser Gly Pro Tyr Gly Gly Gly Gly Gln Tyr Phe Ala Lys Pro 
            20                  25                  30          


Arg Asn Gln Gly Gly Tyr 
        35              


<210>  86
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  86

Arg Met Arg Ile Glx Phe Lys Asn Lys Gly Lys Asp Thr Ala Glu Leu 
1               5                   10                  15      


Arg Arg Arg Arg Val Glu Val Ser Val Glu Leu Arg Lys Ala Lys Lys 
            20                  25                  30          


Asp Glu Gln Ile Leu Lys Arg Arg Asn Val 
        35                  40          


<210>  87
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  87

Val Ser Arg Lys Arg Pro Arg Pro 
1               5               


<210>  88
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  88

Pro Pro Lys Lys Ala Arg Glu Asp 
1               5               


<210>  89
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  89

Pro Gln Pro Lys Lys Lys Pro Leu 
1               5               


<210>  90
<211>  12
<212>  PRT
<213>  Mus musculus

<400>  90

Ser Ala Leu Ile Lys Lys Lys Lys Lys Met Ala Pro 
1               5                   10          


<210>  91
<211>  5
<212>  PRT
<213>  Influenza virus

<400>  91

Asp Arg Leu Arg Arg 
1               5   


<210>  92
<211>  7
<212>  PRT
<213>  Influenza virus

<400>  92

Pro Lys Gln Lys Lys Arg Lys 
1               5           


<210>  93
<211>  10
<212>  PRT
<213>  Hepatitis virus delta

<400>  93

Arg Lys Leu Lys Lys Lys Ile Lys Lys Leu 
1               5                   10  


<210>  94
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  94

Arg Glu Lys Lys Lys Phe Leu Lys Arg Arg 
1               5                   10  


<210>  95
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  95

Lys Arg Lys Gly Asp Glu Val Asp Gly Val Asp Glu Val Ala Lys Lys 
1               5                   10                  15      


Lys Ser Lys Lys 
            20  


<210>  96
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  96

Arg Lys Cys Leu Gln Ala Gly Met Asn Leu Glu Ala Arg Lys Thr Lys 
1               5                   10                  15      


Lys 
    


<210>  97
<211>  23
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (9)..(20)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  N = any nucleotide

<400>  97
mmmmmmmmnn nnnnnnnnnn ngg                                               23


<210>  98
<211>  15
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  N = any nucleotide

<400>  98
nnnnnnnnnn nnngg                                                        15


<210>  99
<211>  23
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (10)..(20)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  N = any nucleotide

<400>  99
mmmmmmmmmn nnnnnnnnnn ngg                                               23


<210>  100
<211>  14
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  N = any nucleotide

<400>  100
nnnnnnnnnn nngg                                                         14


<210>  101
<211>  27
<212>  DNA
<213>  Streptococcus thermophilus


<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (9)..(20)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  w = a or t

<400>  101
mmmmmmmmnn nnnnnnnnnn nnagaaw                                           27


<210>  102
<211>  19
<212>  DNA
<213>  Streptococcus thermophilus


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  w = a or t

<400>  102
nnnnnnnnnn nnnnagaaw                                                    19


<210>  103
<211>  27
<212>  DNA
<213>  Streptococcus thermophilus


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (9)..(20)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  w = a or t

<400>  103
mmmmmmmmmn nnnnnnnnnn nnagaaw                                           27


<210>  104
<211>  18
<212>  DNA
<213>  Streptococcus thermophilus


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (12)..(13)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  w = a or t

<400>  104
nnnnnnnnnn nnnagaaw                                                     18


<210>  105
<211>  25
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (9)..(20)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  N  = any nucleotide

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  N  = any nucleotide

<400>  105
mmmmmmmmnn nnnnnnnnnn nggng                                             25


<210>  106
<211>  17
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  N = any nucleotide

<400>  106
nnnnnnnnnn nnnggng                                                      17


<210>  107
<211>  25
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  m = a or c

<220>
<221>  misc_feature
<222>  (10)..(20)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  N = any nucleotide

<400>  107
mmmmmmmmmn nnnnnnnnnn nggng                                             25


<210>  108
<211>  16
<212>  DNA
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  N is A, G, T, or C

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  N = any nucleotide

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  N = any nucleotide

<400>  108
nnnnnnnnnn nnggng                                                       16


<210>  109
<211>  137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N = any nucleotide

<400>  109
nnnnnnnnnn nnnnnnnnnn gtttttgtac tctcaagatt tagaaataaa tcttgcagaa       60

gctacaaaga taaggcttca tgccgaaatc aacaccctgt cattttatgg cagggtgttt      120

tcgttattta atttttt                                                     137


<210>  110
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or t

<400>  110
nnnnnnnnnn nnnnnnnnnn gtttttgtac tctcagaaat gcagaagcta caaagataag       60

gcttcatgcc gaaatcaaca ccctgtcatt ttatggcagg gtgttttcgt tatttaattt      120

ttt                                                                    123


<210>  111
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N = any nucleotide

<400>  111
nnnnnnnnnn nnnnnnnnnn gtttttgtac tctcagaaat gcagaagcta caaagataag       60

gcttcatgcc gaaatcaaca ccctgtcatt ttatggcagg gtgttttttt                 110


<210>  112
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N = any nucleotide

<400>  112
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  113
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N = any nucleotide

<400>  113
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt gttttttt                                          88


<210>  114
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N = any nucleotide

<400>  114
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcatt tttttt                                                       76


<210>  115
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  115

Met Asp Pro Ile Arg Ser Arg Thr Pro Ser Pro Ala Arg Glu Leu Leu 
1               5                   10                  15      


Ser Gly Pro Gln Pro Asp Gly Val Gln Pro Thr Ala Asp Arg Gly Val 
            20                  25                  30          


Ser Pro Pro Ala Gly Gly Pro Leu Asp Gly Leu Pro Ala Arg Arg Thr 
        35                  40                  45              


Met Ser Arg Thr Arg Leu Pro Ser Pro Pro Ala Pro Ser Pro Ala Phe 
    50                  55                  60                  


Ser Ala Asp Ser Phe Ser Asp Leu Leu Arg Gln Phe Asp Pro Ser Leu 
65                  70                  75                  80  


Phe Asn Thr Ser Leu Phe Asp Ser Leu Pro Pro Phe Gly Ala His His 
                85                  90                  95      


Thr Glu Ala Ala Thr Gly Glu Trp Asp Glu Val Gln Ser Gly Leu Arg 
            100                 105                 110         


Ala Ala Asp Ala Pro Pro Pro Thr Met Arg Val Ala Val Thr Ala Ala 
        115                 120                 125             


Arg Pro Pro Arg Ala Lys Pro Ala Pro Arg Arg Arg Ala Ala Gln Pro 
    130                 135                 140                 


Ser Asp Ala Ser Pro Ala Ala Gln Val Asp Leu Arg Thr Leu Gly Tyr 
145                 150                 155                 160 


Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val Arg Ser Thr Val 
                165                 170                 175     


Ala Gln His His Glu Ala Leu Val Gly His Gly Phe Thr His Ala His 
            180                 185                 190         


Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly Thr Val Ala Val 
        195                 200                 205             


Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu Ala Thr His Glu Ala 
    210                 215                 220                 


Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg Ala Leu Glu Ala 
225                 230                 235                 240 


Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro Pro Leu Gln Leu Asp 
                245                 250                 255     


Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly Gly Val Thr Ala Val 
            260                 265                 270         


Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr Gly Ala Pro Leu Asn 
        275                 280                 285             


<210>  116
<211>  183
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  116

Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro 
1               5                   10                  15      


Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys Leu 
            20                  25                  30          


Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Pro His Ala 
        35                  40                  45              


Pro Ala Leu Ile Lys Arg Thr Asn Arg Arg Ile Pro Glu Arg Thr Ser 
    50                  55                  60                  


His Arg Val Ala Asp His Ala Gln Val Val Arg Val Leu Gly Phe Phe 
65                  70                  75                  80  


Gln Cys His Ser His Pro Ala Gln Ala Phe Asp Asp Ala Met Thr Gln 
                85                  90                  95      


Phe Gly Met Ser Arg His Gly Leu Leu Gln Leu Phe Arg Arg Val Gly 
            100                 105                 110         


Val Thr Glu Leu Glu Ala Arg Ser Gly Thr Leu Pro Pro Ala Ser Gln 
        115                 120                 125             


Arg Trp Asp Arg Ile Leu Gln Ala Ser Gly Met Lys Arg Ala Lys Pro 
    130                 135                 140                 


Ser Pro Thr Ser Thr Gln Thr Pro Asp Gln Ala Ser Leu His Ala Phe 
145                 150                 155                 160 


Ala Asp Ser Leu Glu Arg Asp Leu Asp Ala Pro Ser Pro Met His Glu 
                165                 170                 175     


Gly Asp Gln Thr Arg Ala Ser 
            180             


