                         SEQUENCE LISTING

<110>  KLOGENE THERAPEUTICS, INC.
       THE MEDICAL RESEARCH, INFRASTRUCTURE AND HEALTH SERVICES 
       FUND OF THE TEL-AVIV MEDICAL CENTER
       UNIVERSITAT AUTONOMA DE BARCELONA
       INSTITUT CATALA DE RECERCA I ESTUDIS AVANCATS (ICREA)
       ABRAHAM, Carmela
       ABRAHAM, Menachem
       BOSCH, Assumpcio
       CHILLON, Miguel
       RUBINEK, Tamar
       WOLF, Ido
 
<120>  VECTORS EXPRESSING KLOTHO FOR TREATING CANCER

<130>  SMC/015 PCT

<150>  US 62/720,133
<151>  2018-08-21

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  549
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Pro Ala Ser Ala Pro Pro Arg Arg Pro Arg Pro Pro Pro Pro Ser 
1               5                   10                  15      


Leu Ser Leu Leu Leu Val Leu Leu Gly Leu Gly Gly Arg Arg Leu Arg 
            20                  25                  30          


Ala Glu Pro Gly Asp Gly Ala Gln Thr Trp Ala Arg Val Ser Arg Pro 
        35                  40                  45              


Pro Ala Pro Glu Ala Ala Gly Leu Phe Gln Gly Thr Phe Pro Asp Gly 
    50                  55                  60                  


Phe Leu Trp Ala Val Gly Ser Ala Ala Tyr Gln Thr Glu Gly Gly Trp 
65                  70                  75                  80  


Gln Gln His Gly Lys Gly Ala Ser Ile Trp Asp Thr Phe Thr His His 
                85                  90                  95      


Pro Leu Ala Pro Pro Gly Asp Ser Arg Asn Ala Ser Leu Pro Leu Gly 
            100                 105                 110         


Ala Pro Ser Pro Leu Gln Pro Ala Thr Gly Asp Val Ala Ser Asp Ser 
        115                 120                 125             


Tyr Asn Asn Val Phe Arg Asp Thr Glu Ala Leu Arg Glu Leu Gly Val 
    130                 135                 140                 


Thr His Tyr Arg Phe Ser Ile Ser Trp Ala Arg Val Leu Pro Asn Gly 
145                 150                 155                 160 


Ser Ala Gly Val Pro Asn Arg Glu Gly Leu Arg Tyr Tyr Arg Arg Leu 
                165                 170                 175     


Leu Glu Arg Leu Arg Glu Leu Gly Val Gln Pro Val Val Thr Leu Tyr 
            180                 185                 190         


His Trp Asp Leu Pro Gln Arg Leu Gln Asp Ala Tyr Gly Gly Trp Ala 
        195                 200                 205             


Asn Arg Ala Leu Ala Asp His Phe Arg Asp Tyr Ala Glu Leu Cys Phe 
    210                 215                 220                 


Arg His Phe Gly Gly Gln Val Lys Tyr Trp Ile Thr Ile Asp Asn Pro 
225                 230                 235                 240 


Tyr Val Val Ala Trp His Gly Tyr Ala Thr Gly Arg Leu Ala Pro Gly 
                245                 250                 255     


Ile Arg Gly Ser Pro Arg Leu Gly Tyr Leu Val Ala His Asn Leu Leu 
            260                 265                 270         


Leu Ala His Ala Lys Val Trp His Leu Tyr Asn Thr Ser Phe Arg Pro 
        275                 280                 285             


Thr Gln Gly Gly Gln Val Ser Ile Ala Leu Ser Ser His Trp Ile Asn 
    290                 295                 300                 


Pro Arg Arg Met Thr Asp His Ser Ile Lys Glu Cys Gln Lys Ser Leu 
305                 310                 315                 320 


Asp Phe Val Leu Gly Trp Phe Ala Lys Pro Val Phe Ile Asp Gly Asp 
                325                 330                 335     


Tyr Pro Glu Ser Met Lys Asn Asn Leu Ser Ser Ile Leu Pro Asp Phe 
            340                 345                 350         


Thr Glu Ser Glu Lys Lys Phe Ile Lys Gly Thr Ala Asp Phe Phe Ala 
        355                 360                 365             


Leu Cys Phe Gly Pro Thr Leu Ser Phe Gln Leu Leu Asp Pro His Met 
    370                 375                 380                 


Lys Phe Arg Gln Leu Glu Ser Pro Asn Leu Arg Gln Leu Leu Ser Trp 
385                 390                 395                 400 


Ile Asp Leu Glu Phe Asn His Pro Gln Ile Phe Ile Val Glu Asn Gly 
                405                 410                 415     


Trp Phe Val Ser Gly Thr Thr Lys Arg Asp Asp Ala Lys Tyr Met Tyr 
            420                 425                 430         


Tyr Leu Lys Lys Phe Ile Met Glu Thr Leu Lys Ala Ile Lys Leu Asp 
        435                 440                 445             


Gly Val Asp Val Ile Gly Tyr Thr Ala Trp Ser Leu Met Asp Gly Phe 
    450                 455                 460                 


Glu Trp His Arg Gly Tyr Ser Ile Arg Arg Gly Leu Phe Tyr Val Asp 
465                 470                 475                 480 


Phe Leu Ser Gln Asp Lys Met Leu Leu Pro Lys Ser Ser Ala Leu Phe 
                485                 490                 495     


Tyr Gln Lys Leu Ile Glu Lys Asn Gly Phe Pro Pro Leu Pro Glu Asn 
            500                 505                 510         


Gln Pro Leu Glu Gly Thr Phe Pro Cys Asp Phe Ala Trp Gly Val Val 
        515                 520                 525             


Asp Asn Tyr Ile Gln Val Ser Gln Leu Thr Lys Pro Ile Ser Ser Leu 
    530                 535                 540                 


Thr Lys Pro Tyr His 
545                 


<210>  2
<211>  3008
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV plasmid  ITR-CMV-sKL-PolyA-ITR

<400>  2
aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg       60

ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc      120

gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag ttaatgatta      180

acccgccatg ctacttatct acgtagccat gctctagtta ttaatagtaa ctcaattacg      240

gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc      300

ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc      360

atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt acggtaaact      420

gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat      480

gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact      540

tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac      600

atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac      660

gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac      720

tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga      780

gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac      840

tatagggaga cccaagctgg ctagaatgcc cgccagcgcc ccgccgcgcc gcccgcgtcc      900

accgccgccg tcgctgtcgc tgctgctggt gctgctgggc ctgggcggac gacgcctgcg      960

tgcggagccg ggcgacggcg cgcagacctg ggcccgtgtc tcgcggcctc ctgcccccga     1020

ggccgcgggc ctcttccagg gcaccttccc cgacggcttc ctctgggccg tgggcagcgc     1080

cgcctaccag accgagggcg gctggcagca gcacggcaag ggtgcgtcca tctgggacac     1140

gttcacccac caccccctgg cacccccggg agactcccgg aacgccagtc tgccgttggg     1200

cgccccgtcg ccgctgcagc ccgccaccgg ggacgtagcc agcgacagct acaacaacgt     1260

cttccgcgac acggaggcgc tgcgcgagct cggggtcact cactaccgct tctccatctc     1320

gtgggcgcga gtgctcccca atggcagcgc gggcgtcccc aaccgcgagg ggctgcgcta     1380

ctaccggcgc ctgctggagc ggctgcggga gctgggcgtg cagcccgtgg tcaccctgta     1440

ccactgggac ctgccccagc gcctgcagga cgcctacggc ggctgggcca accgcgccct     1500

ggccgaccac ttcagggatt acgcggagct ctgcttccgc cacttcggcg gtcaggtcaa     1560

gtactggatc accatcgaca acccctacgt ggtggcctgg cacggctacg ccaccgggcg     1620

cctggccccc ggcatccggg gcagcccgcg gctcgggtac ctggtggcgc acaacctcct     1680

cctggctcat gccaaagtct ggcatctcta caatacttct ttccgtccca ctcagggagg     1740

tcaggtgtcc attgccctaa gctctcactg gatcaatcct cgaagaatga ccgaccacag     1800

catcaaagaa tgtcaaaaat ctctggactt tgtactaggt tggtttgcca aacccgtatt     1860

tattgatggt gactatcccg agagcatgaa gaataacctt tcatctattc tgcctgattt     1920

tactgaatct gagaaaaagt tcatcaaagg aactgctgac ttttttgctc tttgctttgg     1980

acccaccttg agttttcaac ttttggaccc tcacatgaag ttccgccaat tggaatctcc     2040

caacctgagg caactgcttt cctggattga ccttgaattt aaccatcctc aaatatttat     2100

tgtggaaaat ggctggtttg tctcagggac caccaagaga gatgatgcca aatatatgta     2160

ttacctcaaa aagttcatca tggaaacctt aaaagccatc aagctggatg gggtggatgt     2220

catcgggtat accgcatggt ccctcatgga tggtttcgag tggcacagag gttacagcat     2280

caggcgtgga ctcttctatg ttgactttct aagccaggac aagatgttgt tgccaaagtc     2340

ttcagccttg ttctaccaaa agctgataga gaaaaatggc ttccctcctt tacctgaaaa     2400

tcagccccta gaagggacat ttccctgtga ctttgcttgg ggagttgtcg acaactacat     2460

tcaagtaagt cagctgacaa aaccaatcag cagtctcacc aagccctatc actagtaagc     2520

ggccgcttcc ctttagtgag ggttaatgct tcgagcagac atgataagat acattgatga     2580

gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga     2640

tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg     2700

cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa     2760

cctctacaaa tgtggtaaaa tccgataagg gactagagca tggctacgta gataagtagc     2820

atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc     2880

tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg     2940

cccgggcggc ctcagagagc gagcgagcgc gcagagaggg agtggccaac tccatcacta     3000

gggttcct                                                              3008


<210>  3
<211>  1913
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RSV-sKL construct

<400>  3
aatgtagtct tatgcaatac acttgtagtc ttgcaacatg gtaacgatga gttagcaaca       60

tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga      120

tcgtgcctta ttaggaaggc aacagacagg tctgacatgg attggacgaa ccactgaatt      180

ccgcattgca gagataattg tatttaagtg cctagctcga tacaataaac gccatttgac      240

cattcaccac attggtgtgc accatgcccg ccagcgcccc gccgcgccgc ccgcggccgc      300

cgccgccgtc gctgtcgctg ctgctggtgc tgctgggcct gggcggccgc cgcctgcgtg      360

cggagccggg cgacggcgcg cagacctggg cccgtgtctc gcggcctcct gcccccgagg      420

ccgcgggcct cttccagggc accttccccg acggcttcct ctgggccgtg ggcagcgccg      480

cctaccagac cgagggcggc tggcagcagc acggcaaggg tgcgtccatc tgggacacgt      540

tcacccacca ccccctggca cccccgggag actcccggaa cgccagtctg ccgttgggcg      600

ccccgtcgcc gctgcagccc gccaccgggg acgtagccag cgacagctac aacaacgtct      660

tccgcgacac ggaggcgctg cgcgagctcg gggtcactca ctaccgcttc tccatctcgt      720

gggcgcgagt gctccccaat ggcagcgcgg gcgtccccaa ccgcgagggg ctgcgctact      780

accggcgcct gctggagcgg ctgcgggagc tgggcgtgca gcccgtggtc accctgtacc      840

actgggacct gccccagcgc ctgcaggacg cctacggcgg ctgggccaac cgcgccctgg      900

ccgaccactt cagggattac gcggagctct gcttccgcca cttcggcggt caggtcaagt      960

actggatcac catcgacaac ccctacgtgg tggcctggca cggctacgcc accgggcgcc     1020

tggcccccgg catccggggc agcccgcggc tcgggtacct ggtggcgcac aacctcctcc     1080

tggctcatgc caaagtctgg catctctaca atacttcttt ccgtcccact cagggaggtc     1140

aggtgtccat tgccctaagc tctcactgga tcaatcctcg aagaatgacc gaccacagca     1200

tcaaagaatg tcaaaaatct ctggactttg tactaggttg gtttgccaaa cccgtattta     1260

ttgatggtga ctatcccgag agcatgaaga ataacctttc atctattctg cctgatttta     1320

ctgaatctga gaaaaagttc atcaaaggaa ctgctgactt ttttgctctt tgctttggac     1380

ccaccttgag ttttcaactt ttggaccctc acatgaagtt ccgccaattg gaatctccca     1440

acctgaggca actgctttcc tggattgacc ttgaatttaa ccatcctcaa atatttattg     1500

tggaaaatgg ctggtttgtc tcagggacca ccaagagaga tgatgccaaa tatatgtatt     1560

acctcaaaaa gttcatcatg gaaaccttaa aagccatcaa gctggatggg gtggatgtca     1620

tcgggtatac cgcatggtcc ctcatggatg gtttcgagtg gcacagaggt tacagcatca     1680

ggcgtggact cttctatgtt gactttctaa gccaggacaa gatgttgttg ccaaagtctt     1740

cagccttgtt ctaccaaaag ctgatagaga aaaatggctt ccctccttta cctgaaaatc     1800

agcccctaga agggacattt ccctgtgact ttgcttgggg agttgttgac aactacattc     1860

aagtaagtca gctgacaaaa ccaatcagca gtctcaccaa gccctatcac tag            1913


<210>  4
<211>  2640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Desmin-sKL construct

<400>  4
cggccgcacc catgcctcct caggtacccc ctgcccccca cagctcctct cctgtgcctt       60

gtttcccagc catgcgttct cctctataaa tacccgctct ggtatttggg gttggcagct      120

gttgctgcca gggagatggt tgggttgaca tgcggctcct gacaaaacac aaacccctgg      180

tgtgtgtggg cgtgggtggt gtgagtaggg ggatgaatca gggagggggc gggggaccca      240

gggggcagga gccacacaaa gtctgtgcgg gggtgggagc gcacatagca attggaaact      300

gaaagcttat cagacccttt ctggaaatca gcccactgtt tataaacttg aggccccacc      360

ctcgagataa ccagggctga aagaggcccg cctgggggct gcagacatgc ttgctgcctg      420

ccctggcgaa ggattggcag gcttgcccgt cacaggaccc ccgctggctg actcaggggc      480

gcaggcctct tgcgggggag ctggcctccc cgcccccacg gccacgggcc gccctttcct      540

ggcaggacag cgggatcttg cagctgtcag gggaggggag gcgggggctg atgtcaggag      600

ggatacaaat agtgccgacg gctgggggcc ctgtctcccc tcgccgcatc cactctccgg      660

ccggccgcct gcccgccgcc tcctccgtgc gcccgccagc ctcgcccgcg ccgtcaccgg      720

ttcgaacagg taagcgcccc taaaatccct ttggcacaat gtgtcctgag gggagaggca      780

gcgacctgta gatgggacgg gggcactaac cctcagggtt tggggttctg aatgtgagta      840

tcgccatcta agcccagtat ttggccaatc tcagaaagct cctggctccc tggaggatgg      900

agagagaaaa acaaacagct cctggagcag ggagagtgtt ggcctcttgc tctccggctc      960

cctctgttgc cctctggttt ctccccaggt atgcccgcca gcgccccgcc gcgccgcccg     1020

cggccgccgc cgccgtcgct gtcgctgctg ctggtgctgc tgggcctggg cggccgccgc     1080

ctgcgtgcgg agccgggcga cggcgcgcag acctgggccc gtgtctcgcg gcctcctgcc     1140

cccgaggccg cgggcctctt ccagggcacc ttccccgacg gcttcctctg ggccgtgggc     1200

agcgccgcct accagaccga gggcggctgg cagcagcacg gcaagggtgc gtccatctgg     1260

gacacgttca cccaccaccc cctggcaccc ccgggagact cccggaacgc cagtctgccg     1320

ttgggcgccc cgtcgccgct gcagcccgcc accggggacg tagccagcga cagctacaac     1380

aacgtcttcc gcgacacgga ggcgctgcgc gagctcgggg tcactcacta ccgcttctcc     1440

atctcgtggg cgcgagtgct ccccaatggc agcgcgggcg tccccaaccg cgaggggctg     1500

cgctactacc ggcgcctgct ggagcggctg cgggagctgg gcgtgcagcc cgtggtcacc     1560

ctgtaccact gggacctgcc ccagcgcctg caggacgcct acggcggctg ggccaaccgc     1620

gccctggccg accacttcag ggattacgcg gagctctgct tccgccactt cggcggtcag     1680

gtcaagtact ggatcaccat cgacaacccc tacgtggtgg cctggcacgg ctacgccacc     1740

gggcgcctgg cccccggcat ccggggcagc ccgcggctcg ggtacctggt ggcgcacaac     1800

ctcctcctgg ctcatgccaa agtctggcat ctctacaata cttctttccg tcccactcag     1860

ggaggtcagg tgtccattgc cctaagctct cactggatca atcctcgaag aatgaccgac     1920

cacagcatca aagaatgtca aaaatctctg gactttgtac taggttggtt tgccaaaccc     1980

gtatttattg atggtgacta tcccgagagc atgaagaata acctttcatc tattctgcct     2040

gattttactg aatctgagaa aaagttcatc aaaggaactg ctgacttttt tgctctttgc     2100

tttggaccca ccttgagttt tcaacttttg gaccctcaca tgaagttccg ccaattggaa     2160

tctcccaacc tgaggcaact gctttcctgg attgaccttg aatttaacca tcctcaaata     2220

tttattgtgg aaaatggctg gtttgtctca gggaccacca agagagatga tgccaaatat     2280

atgtattacc tcaaaaagtt catcatggaa accttaaaag ccatcaagct ggatggggtg     2340

gatgtcatcg ggtataccgc atggtccctc atggatggtt tcgagtggca cagaggttac     2400

agcatcaggc gtggactctt ctatgttgac tttctaagcc aggacaagat gttgttgcca     2460

aagtcttcag ccttgttcta ccaaaagctg atagagaaaa atggcttccc tcctttacct     2520

gaaaatcagc ccctagaagg gacatttccc tgtgactttg cttggggagt tgttgacaac     2580

tacattcaag taagtcagct gacaaaacca atcagcagtc tcaccaagcc ctatcactag     2640


<210>  5
<211>  263
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RSV promoter

<400>  5
aatgtagtct tatgcaatac acttgtagtc ttgcaacatg gtaacgatga gttagcaaca       60

tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga      120

tcgtgcctta ttaggaaggc aacagacagg tctgacatgg attggacgaa ccactgaatt      180

ccgcattgca gagataattg tatttaagtg cctagctcga tacaataaac gccatttgac      240

cattcaccac attggtgtgc acc                                              263


<210>  6
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Desmin promoter

<400>  6
cggccgcacc catgcctcct caggtacccc ctgcccccca cagctcctct cctgtgcctt       60

gtttcccagc catgcgttct cctctataaa tacccgctct ggtatttggg gttggcagct      120

gttgctgcca gggagatggt tgggttgaca tgcggctcct gacaaaacac aaacccctgg      180

tgtgtgtggg cgtgggtggt gtgagtaggg ggatgaatca gggagggggc gggggaccca      240

gggggcagga gccacacaaa gtctgtgcgg gggtgggagc gcacatagca attggaaact      300

gaaagcttat cagacccttt ctggaaatca gcccactgtt tataaacttg aggccccacc      360

ctcgagataa ccagggctga aagaggcccg cctgggggct gcagacatgc ttgctgcctg      420

ccctggcgaa ggattggcag gcttgcccgt cacaggaccc ccgctggctg actcaggggc      480

gcaggcctct tgcgggggag ctggcctccc cgcccccacg gccacgggcc gccctttcct      540

ggcaggacag cgggatcttg cagctgtcag gggaggggag gcgggggctg atgtcaggag      600

ggatacaaat agtgccgacg gctgggggcc ctgtctcccc tcgccgcatc cactctccgg      660

ccggccgcct gcccgccgcc tcctccgtgc gcccgccagc ctcgcccgcg ccgtcaccgg      720

ttcgaacagg taagcgcccc taaaatccct ttggcacaat gtgtcctgag gggagaggca      780

gcgacctgta gatgggacgg gggcactaac cctcagggtt tggggttctg aatgtgagta      840

tcgccatcta agcccagtat ttggccaatc tcagaaagct cctggctccc tggaggatgg      900

agagagaaaa acaaacagct cctggagcag ggagagtgtt ggcctcttgc tctccggctc      960

cctctgttgc cctctggttt ctccccaggt                                       990


<210>  7
<211>  4302
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pRepCap9 plasmid

<400>  7
atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc       60

ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat      120

tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag      180

cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggctct tttctttgtg      240

caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg      300

aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt      360

taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc      420

gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa      480

acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg      540

aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag      600

gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact      660

tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag      720

cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg      780

tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc      840

cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa      900

attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc      960

acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag     1020

accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc     1080

aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg ggaggagggg     1140

aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc     1200

gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc     1260

aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg     1320

ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag     1380

gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg     1440

gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca     1500

gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg     1560

gaagcttcga tcaactacgc ggacaggtac caaaacaaat gttctcgtca cgtgggcatg     1620

aatctgatgc tgtttccctg cagacaatgc gagagactga atcagaattc aaatatctgc     1680

ttcactcacg gtgtcaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt     1740

tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcacatcat gggaaaggtg     1800

ccagacgctt gcactgcttg cgacctggtc aatgtggact tggatgactg tgtttctgaa     1860

caataaatga cttaaaccag gtatggctgc cgatggttat cttccagatt ggctcgagga     1920

caaccttagt gaaggaattc gcgagtggtg ggctttgaaa cctggagccc ctcaacccaa     1980

ggcaaatcaa caacatcaag acaacgctcg aggtcttgtg cttccgggtt acaaatacct     2040

tggacccggc aacggactcg acaaggggga gccggtcaac gcagcagacg cggcggccct     2100

cgagcacgac aaggcctacg accagcagct caaggccgga gacaacccgt acctcaagta     2160

caaccacgcc gacgccgagt tccaggagcg gctcaaagaa gatacgtctt ttgggggcaa     2220

cctcgggcga gcagtcttcc aggccaaaaa gaggcttctt gaacctcttg gtctggttga     2280

ggaagcggct aagacggctc ctggaaagaa gaggcctgta gagcagtctc ctcaggaacc     2340

ggactcctcc gcgggtattg gcaaatcggg tgcacagccc gctaaaaaga gactcaattt     2400

cggtcagact ggcgacacag agtcagtccc agaccctcaa ccaatcggag aacctcccgc     2460

agccccctca ggtgtgggat ctcttacaat ggcttcaggt ggtggcgcac cagtggcaga     2520

caataacgaa ggtgccgatg gagtgggtag ttcctcggga aattggcatt gcgattccca     2580

atggctgggg gacagagtca tcaccaccag cacccgaacc tgggccctgc ccacctacaa     2640

caatcacctc tacaagcaaa tctccaacag cacatctgga ggatcttcaa atgacaacgc     2700

ctacttcggc tacagcaccc cctgggggta ttttgacttc aacagattcc actgccactt     2760

ctcaccacgt gactggcagc gactcatcaa caacaactgg ggattccggc ctaagcgact     2820

caacttcaag ctcttcaaca ttcaggtcaa agaggttacg gacaacaatg gagtcaagac     2880

catcgccaat aaccttacca gcacggtcca ggtcttcacg gactcagact atcagctccc     2940

gtacgtgctc gggtcggctc acgagggctg cctcccgccg ttcccagcgg acgttttcat     3000

gattcctcag tacgggtatc tgacgcttaa tgatggaagc caggccgtgg gtcgttcgtc     3060

cttttactgc ctggaatatt tcccgtcgca aatgctaaga acgggtaaca acttccagtt     3120

cagctacgag tttgagaacg tacctttcca tagcagctac gctcacagcc aaagcctgga     3180

ccgactaatg aatccactca tcgaccaata cttgtactat ctctcaaaga ctattaacgg     3240

ttctggacag aatcaacaaa cgctaaaatt cagtgtggcc ggacccagca acatggctgt     3300

ccagggaaga aactacatac ctggacccag ctaccgacaa caacgtgtct caaccactgt     3360

gactcaaaac aacaacagcg aatttgcttg gcctggagct tcttcttggg ctctcaatgg     3420

acgtaatagc ttgatgaatc ctggacctgc tatggccagc cacaaagaag gagaggaccg     3480

tttctttcct ttgtctggat ctttaatttt tggcaaacaa ggaactggaa gagacaacgt     3540

ggatgcggac aaagtcatga taaccaacga agaagaaatt aaaactacta acccggtagc     3600

aacggagtcc tatggacaag tggccacaaa ccaccagagt gcccaagcac aggcgcagac     3660

cggctgggtt caaaaccaag gaatacttcc gggtatggtt tggcaggaca gagatgtgta     3720

cctgcaagga cccatttggg ccaaaattcc tcacacggac ggcaactttc acccttctcc     3780

gctgatggga gggtttggaa tgaagcaccc gcctcctcag atcctcatca aaaacacacc     3840

tgtacctgcg gatcctccaa cggccttcaa caaggacaag ctgaactctt tcatcaccca     3900

gtattctact ggccaagtca gcgtggagat cgagtgggag ctgcagaagg aaaacagcaa     3960

gcgctggaac ccggagatcc agtacacttc caactattac aagtctaata atgttgaatt     4020

tgctgttaat actgaaggtg tatatagtga accccgcccc attggcacca gatacctgac     4080

tcgtaatctg taattgcttg ttaatcaata aaccgtttaa ttcgtttcag ttgaactttg     4140

gtctctgcat atggcgaatt cgtttaaacc tgcaggacta gagtcctgta ttagaggtca     4200

cgtgagtgtt ttgcgacatt ttgcgacacc atgtggtcac gctgggtatt taagcccgag     4260

tgagcacgca gggtctccat tttgaagcgg gaggtttgaa cg                        4302


<210>  8
<211>  4278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pRepCap for AAV-5

<400>  8
atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacgg gcatctgccc       60

ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat      120

tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag      180

cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct tttctttgtg      240

caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg      300

aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt      360

taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc      420

gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa      480

acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg      540

aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag      600

gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact      660

tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag      720

cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg      780

tcccaaatca aggtgccttg gacaatgcgg gaaagattat gagcctgact aaaaccgccc      840

ccgactacct ggtgggccag cagcccgtgg aggacatttc cagcaatcgg atttataaaa      900

ttttggaact aaacgggtac gatccccaat atgcggcttc cgtctttctg ggatgggcca      960

cgaaaaagtt cggcaagagg aacaccatct ggctgtttgg gcctgcaact accgggaaga     1020

ccaacatcgc ggaggccata gcccacactg tgcccttcta cgggtgcgta aactggacca     1080

atgagaactt tcccttcaac gactgtgtcg acaagatggt gatctggtgg gaggagggga     1140

agatgaccgc caaggtcgtg gagtcggcca aagccattct cggaggaagc aaggtgcgcg     1200

tggaccagaa atgcaagtcc tcggcccaga tagacccgac tcccgtgatc gtcacctcca     1260

acaccaacat gtgcgccgtg attgacggga actcaacgac cttcgaacac cagcagccgt     1320

tgcaagaccg gatgttcaaa tttgaactca cccgccgtct ggatcatgac tttgggaagg     1380

tcaccaagca ggaagtcaaa gactttttcc ggtgggcaaa ggatcacgtg gttgaggtgg     1440

agcatgaatt ctacgtcaaa aagggtggag ccaagaaaag acccgccccc agtgacgcag     1500

atataagtga gcccaaacgg gtgcgcgagt cagttgcgca gccatcgacg tcagacgcgg     1560

aagcttcgat caactacgca gacaggtacc aaaacaaatg ttctcgtcac gtgggcatga     1620

atctgatgct gtttccctgc agacaatgcg agagaatgaa tcagaattca aatatctgct     1680

tcactcacgg acagaaagac tgtttagagt gctttcccgt gtcagaatct caacccgttt     1740

ctgtcgtcaa aaaggcgtat cagaaactgt gctacattca tcatatcatg ggaaaggtgc     1800

cagacgcttg cactgcctgc gatctggtca atgtggattt ggatgactgc atctttgaac     1860

aataaatgat ttaaatcagg tatggctgcc gatggttatc ttccagattg gctcgacgac     1920

aacctctctg agggcattcg cgagtggtgg gacctgaaac ctggagcccc gaaacccaaa     1980

gccaaccagc aaaagcagga caacggccgg ggtctggtgc ttcctggcta caagtacctc     2040

ggacccttca acggactcga caagggggag cccgtcaacg cggcggacgc agcggccgtc     2100

gacatgtctt ttgttgatca ccctccagat tggttggaag aagttggtga aggtcttcgc     2160

gagtttttgg gccttgaagc gggcccaccg aaaccaaaac ccaatcagca gcatcaagat     2220

caagcccgtg gtcttgtgct gcctggttat aactatctcg gacccggaaa cggtctcgat     2280

cgaggagagc ctgtcaacag ggcagacgag gtcgcgcgag agcacgacat ctcgtacaac     2340

gagcagcttg aggcgggaga caacccctac ctcaagtaca accacgcgga cgccgagttt     2400

caggagaagc tcgccgacga cacatccttc gggggaaacc tcggaaaggc agtctttcag     2460

gccaagaaaa gggttctcga accttttggc ctggttgaag agggtgctaa gacggcccct     2520

accggaaagc ggatagacga ccactttcca aaaagaaaga aggctcggac cgaagaggac     2580

tccaagcctt ccacctcgtc agacgccgaa gctggaccca gcggatccca gcagctgcaa     2640

atcccagccc aaccagcctc aagtttggga gctgatacaa tgtctgcggg aggtggcggc     2700

ccattgggcg acaataacca aggtgccgat ggagtgggca atgcctcggg agattggcat     2760

tgcgattcca cgtggatggg ggacagagtc gtcaccaagt ccacccgaac ctgggtgctg     2820

cccagctaca acaaccacca gtaccgagag atcaaaagcg gctccgtcga cggaagcaac     2880

gccaacgcct actttggata cagcaccccc tgggggtact ttgactttaa ccgcttccac     2940

agccactgga gcccccgaga ctggcaaaga ctcatcaaca actactgggg cttcagaccc     3000

cggtccctca gagtcaaaat cttcaacatt caagtcaaag aggtcacggt gcaggactcc     3060

accaccacca tcgccaacaa cctcacctcc accgtccaag tgtttacgga cgacgactac     3120

cagctgccct acgtcgtcgg caacgggacc gagggatgcc tgccggcctt ccctccgcag     3180

gtctttacgc tgccgcagta cggttacgcg acgctgaacc gcgacaacac agaaaatccc     3240

accgagagga gcagcttctt ctgcctagag tactttccca gcaagatgct gagaacgggc     3300

aacaactttg agtttaccta caactttgag gaggtgccct tccactccag cttcgctccc     3360

agtcagaacc tgttcaagct ggccaacccg ctggtggacc agtacttgta ccgcttcgtg     3420

agcacaaata acactggcgg agtccagttc aacaagaacc tggccgggag atacgccaac     3480

acctacaaaa actggttccc ggggcccatg ggccgaaccc agggctggaa cctgggctcc     3540

ggggtcaacc gcgccagtgt cagcgccttc gccacgacca ataggatgga gctcgagggc     3600

gcgagttacc aggtgccccc gcagccgaac ggcatgacca acaacctcca gggcagcaac     3660

acctatgccc tggagaacac tatgatcttc aacagccagc cggcgaaccc gggcaccacc     3720

gccacgtacc tcgagggcaa catgctcatc accagcgaga gcgagacgca gccggtgaac     3780

cgcgtggcgt acaacgtcgg cgggcagatg gccaccaaca accagagctc caccactgcc     3840

cccgcgaccg gcacgtacaa cctccaggaa atcgtgcccg gcagcgtgtg gatggagagg     3900

gacgtgtacc tccaaggacc catctgggcc aagatcccag agacgggggc gcactttcac     3960

ccctctccgg ccatgggcgg attcggactc aaacacccac cgcccatgat gctcatcaag     4020

aacacgcctg tgcccggaaa tatcaccagc ttctcggacg tgcccgtcag cagcttcatc     4080

acccagtaca gcaccgggca ggtcaccgtg gagatggagt gggagctcaa gaaggaaaac     4140

tccaagaggt ggaacccaga gatccagtac acaaacaact acaacgaccc ccagtttgtg     4200

gactttgccc cggacagcac cggggaatac agaaccacca gacctatcgg aacccgatac     4260

cttacccgac ccctttaa                                                   4278


<210>  9
<211>  1012
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Pro Ala Ser Ala Pro Pro Arg Arg Pro Arg Pro Pro Pro Gln Ser 
1               5                   10                  15      


Leu Ser Leu Leu Leu Val Leu Leu Gly Leu Gly Gly Arg Arg Leu Arg 
            20                  25                  30          


Ala Glu Pro Gly Asp Gly Ala Gln Thr Trp Ala Arg Phe Ser Arg Pro 
        35                  40                  45              


Pro Ala Pro Glu Ala Ala Gly Leu Phe Gln Gly Thr Phe Pro Asp Gly 
    50                  55                  60                  


Phe Leu Trp Ala Val Gly Ser Ala Ala Tyr Gln Thr Glu Gly Gly Trp 
65                  70                  75                  80  


Gln Gln His Gly Lys Gly Ala Ser Ile Trp Asp Thr Phe Thr His His 
                85                  90                  95      


Pro Leu Ala Pro Pro Gly Asp Ser Arg Asn Ala Ser Leu Pro Leu Gly 
            100                 105                 110         


Ala Pro Ser Pro Leu Gln Pro Ala Thr Gly Asp Val Ala Ser Asp Ser 
        115                 120                 125             


Tyr Asn Asn Val Phe Arg Asp Thr Glu Ala Leu Arg Glu Leu Gly Val 
    130                 135                 140                 


Thr His Tyr Arg Phe Ser Ile Ser Trp Ala Arg Val Leu Pro Asn Gly 
145                 150                 155                 160 


Ser Ala Gly Val Pro Asn Arg Glu Gly Leu Arg Tyr Tyr Arg Arg Leu 
                165                 170                 175     


Leu Glu Arg Leu Arg Glu Leu Gly Val Gln Pro Val Val Thr Leu Tyr 
            180                 185                 190         


His Trp Asp Leu Pro Gln Arg Leu Gln Asp Ala Tyr Gly Gly Trp Ala 
        195                 200                 205             


Asn Arg Ala Leu Ala Asp His Phe Arg Asp Tyr Ala Glu Leu Cys Phe 
    210                 215                 220                 


Arg His Phe Gly Gly Gln Val Lys Tyr Trp Ile Thr Ile Asp Asn Pro 
225                 230                 235                 240 


Tyr Val Val Ala Trp His Gly Tyr Ala Thr Gly Arg Leu Ala Pro Gly 
                245                 250                 255     


Ile Arg Gly Ser Pro Arg Leu Gly Tyr Leu Val Ala His Asn Leu Leu 
            260                 265                 270         


Leu Ala His Ala Lys Val Trp His Leu Tyr Asn Thr Ser Phe Arg Pro 
        275                 280                 285             


Thr Gln Gly Gly Gln Val Ser Ile Ala Leu Ser Ser His Trp Ile Asn 
    290                 295                 300                 


Pro Arg Arg Met Thr Asp His Ser Ile Lys Glu Cys Gln Lys Ser Leu 
305                 310                 315                 320 


Asp Phe Val Leu Gly Trp Phe Ala Lys Pro Val Phe Ile Asp Gly Asp 
                325                 330                 335     


Tyr Pro Glu Ser Met Lys Asn Asn Leu Ser Ser Ile Leu Pro Asp Phe 
            340                 345                 350         


Thr Glu Ser Glu Lys Lys Phe Ile Lys Gly Thr Ala Asp Phe Phe Ala 
        355                 360                 365             


Leu Cys Phe Gly Pro Thr Leu Ser Phe Gln Leu Leu Asp Pro His Met 
    370                 375                 380                 


Lys Phe Arg Gln Leu Glu Ser Pro Asn Leu Arg Gln Leu Leu Ser Trp 
385                 390                 395                 400 


Ile Asp Leu Glu Phe Asn His Pro Gln Ile Phe Ile Val Glu Asn Gly 
                405                 410                 415     


Trp Phe Val Ser Gly Thr Thr Lys Arg Asp Asp Ala Lys Tyr Met Tyr 
            420                 425                 430         


Tyr Leu Lys Lys Phe Ile Met Glu Thr Leu Lys Ala Ile Lys Leu Asp 
        435                 440                 445             


Gly Val Asp Val Ile Gly Tyr Thr Ala Trp Ser Leu Met Asp Gly Phe 
    450                 455                 460                 


Glu Trp His Arg Gly Tyr Ser Ile Arg Arg Gly Leu Phe Tyr Val Asp 
465                 470                 475                 480 


Phe Leu Ser Gln Asp Lys Met Leu Leu Pro Lys Ser Ser Ala Leu Phe 
                485                 490                 495     


Tyr Gln Lys Leu Ile Glu Lys Asn Gly Phe Pro Pro Leu Pro Glu Asn 
            500                 505                 510         


Gln Pro Leu Glu Gly Thr Phe Pro Cys Asp Phe Ala Trp Gly Val Val 
        515                 520                 525             


Asp Asn Tyr Ile Gln Val Asp Thr Thr Leu Ser Gln Phe Thr Asp Leu 
    530                 535                 540                 


Asn Val Tyr Leu Trp Asp Val His His Ser Lys Arg Leu Ile Lys Val 
545                 550                 555                 560 


Asp Gly Val Val Thr Lys Lys Arg Lys Ser Tyr Cys Val Asp Phe Ala 
                565                 570                 575     


Ala Ile Gln Pro Gln Ile Ala Leu Leu Gln Glu Met His Val Thr His 
            580                 585                 590         


Phe Arg Phe Ser Leu Asp Trp Ala Leu Ile Leu Pro Leu Gly Asn Gln 
        595                 600                 605             


Ser Gln Val Asn His Thr Ile Leu Gln Tyr Tyr Arg Cys Met Ala Ser 
    610                 615                 620                 


Glu Leu Val Arg Val Asn Ile Thr Pro Val Val Ala Leu Trp Gln Pro 
625                 630                 635                 640 


Met Ala Pro Asn Gln Gly Leu Pro Arg Leu Leu Ala Arg Gln Gly Ala 
                645                 650                 655     


Trp Glu Asn Pro Tyr Thr Ala Leu Ala Phe Ala Glu Tyr Ala Arg Leu 
            660                 665                 670         


Cys Phe Gln Glu Leu Gly His His Val Lys Leu Trp Ile Thr Met Asn 
        675                 680                 685             


Glu Pro Tyr Thr Arg Asn Met Thr Tyr Ser Ala Gly His Asn Leu Leu 
    690                 695                 700                 


Lys Ala His Ala Leu Ala Trp His Val Tyr Asn Glu Lys Phe Arg His 
705                 710                 715                 720 


Ala Gln Asn Gly Lys Ile Ser Ile Ala Leu Gln Ala Asp Trp Ile Glu 
                725                 730                 735     


Pro Ala Cys Pro Phe Ser Gln Lys Asp Lys Glu Val Ala Glu Arg Val 
            740                 745                 750         


Leu Glu Phe Asp Ile Gly Trp Leu Ala Glu Pro Ile Phe Gly Ser Gly 
        755                 760                 765             


Asp Tyr Pro Trp Val Met Arg Asp Trp Leu Asn Gln Arg Asn Asn Phe 
    770                 775                 780                 


Leu Leu Pro Tyr Phe Thr Glu Asp Glu Lys Lys Leu Ile Gln Gly Thr 
785                 790                 795                 800 


Phe Asp Phe Leu Ala Leu Ser His Tyr Thr Thr Ile Leu Val Asp Ser 
                805                 810                 815     


Glu Lys Glu Asp Pro Ile Lys Tyr Asn Asp Tyr Leu Glu Val Gln Glu 
            820                 825                 830         


Met Thr Asp Ile Thr Trp Leu Asn Ser Pro Ser Gln Val Ala Val Val 
        835                 840                 845             


Pro Trp Gly Leu Arg Lys Val Leu Asn Trp Leu Lys Phe Lys Tyr Gly 
    850                 855                 860                 


Asp Leu Pro Met Tyr Ile Ile Ser Asn Gly Ile Asp Asp Gly Leu His 
865                 870                 875                 880 


Ala Glu Asp Asp Gln Leu Arg Val Tyr Tyr Met Gln Asn Tyr Ile Asn 
                885                 890                 895     


Glu Ala Leu Lys Ala His Ile Leu Asp Gly Ile Asn Leu Cys Gly Tyr 
            900                 905                 910         


Phe Ala Tyr Ser Phe Asn Asp Arg Thr Ala Pro Arg Phe Gly Leu Tyr 
        915                 920                 925             


Arg Tyr Ala Ala Asp Gln Phe Glu Pro Lys Ala Ser Met Lys His Tyr 
    930                 935                 940                 


Arg Lys Ile Ile Asp Ser Asn Gly Phe Pro Gly Pro Glu Thr Leu Glu 
945                 950                 955                 960 


Arg Phe Cys Pro Glu Glu Phe Thr Val Cys Thr Glu Cys Ser Phe Phe 
                965                 970                 975     


His Thr Arg Lys Ser Leu Leu Ala Phe Ile Ala Phe Leu Phe Phe Ala 
            980                 985                 990         


Ser Ile Ile Ser Leu Ser Leu Ile  Phe Tyr Tyr Ser Lys  Lys Gly Arg 
        995                 1000                 1005             


Arg Ser  Tyr Lys 
    1010         


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GFP PCR primer R

<400>  10
tcaggtagtg gttgtcgggc a                                                 21


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GFP PCR primer F

<400>  11
aagcagaaga acggcatcaa ggtg                                              24


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta-actin primer F

<400>  12
tgttaccaac tgggacgaca                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta-actin primer R

<400>  13
ggggtgttga aggtctcaaa                                                   20


