                         SEQUENCE LISTING

<110>  GlaxoSmithKline Biologicals s.a.
 
<120>  IMMUNOGENIC PROTEINS AND COMPOSITIONS

<130>  VB66645 WO

<150>  EP 18190597.7
<151>  2018-08-23

<150>  EP 18190822.9
<151>  2018-08-24

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  1706
<212>  PRT
<213>  Bordetella pertussis

<400>  1

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Ile Asp Met Phe 
            180                 185                 190         


Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg Ser Ser 
        195                 200                 205             


Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr Arg Arg 
    210                 215                 220                 


Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile Asp Leu 
225                 230                 235                 240 


Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr Glu 
                245                 250                 255     


Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val Ile 
            260                 265                 270         


Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala His 
        275                 280                 285             


Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln Asn Asn 
    290                 295                 300                 


Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr Gly 
305                 310                 315                 320 


Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile Gly Gln 
                325                 330                 335     


Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr Gly Val 
            340                 345                 350         


Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro Gly Val 
        355                 360                 365             


Pro Ser Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr Val Pro 
    370                 375                 380                 


Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu Arg Gln 
385                 390                 395                 400 


Asp Ser Gly Tyr Asp Ser Leu Asp Gly Val Gly Ser Arg Ser Phe Ser 
                405                 410                 415     


Leu Gly Glu Val Ser Asp Met Ala Ala Val Glu Ala Ala Glu Leu Glu 
            420                 425                 430         


Met Thr Arg Gln Val Leu His Ala Gly Ala Arg Gln Asp Asp Ala Glu 
        435                 440                 445             


Pro Gly Val Ser Gly Ala Ser Ala His Trp Gly Gln Arg Ala Leu Gln 
    450                 455                 460                 


Gly Ala Gln Ala Val Ala Ala Ala Gln Arg Leu Val His Ala Ile Ala 
465                 470                 475                 480 


Leu Met Thr Gln Phe Gly Arg Ala Gly Ser Thr Asn Thr Pro Gln Glu 
                485                 490                 495     


Ala Ala Ser Leu Ser Ala Ala Val Phe Gly Leu Gly Glu Ala Ser Ser 
            500                 505                 510         


Ala Val Ala Glu Thr Val Ser Gly Phe Phe Arg Gly Ser Ser Arg Trp 
        515                 520                 525             


Ala Gly Gly Phe Gly Val Ala Gly Gly Ala Met Ala Leu Gly Gly Gly 
    530                 535                 540                 


Ile Ala Ala Ala Val Gly Ala Gly Met Ser Leu Thr Asp Asp Ala Pro 
545                 550                 555                 560 


Ala Gly Gln Lys Ala Ala Ala Gly Ala Glu Ile Ala Leu Gln Leu Thr 
                565                 570                 575     


Gly Gly Thr Val Glu Leu Ala Ser Ser Ile Ala Leu Ala Leu Ala Ala 
            580                 585                 590         


Ala Arg Gly Val Thr Ser Gly Leu Gln Val Ala Gly Ala Ser Ala Gly 
        595                 600                 605             


Ala Ala Ala Gly Ala Leu Ala Ala Ala Leu Ser Pro Met Glu Ile Tyr 
    610                 615                 620                 


Gly Leu Val Gln Gln Ser His Tyr Ala Asp Gln Leu Asp Lys Leu Ala 
625                 630                 635                 640 


Gln Glu Ser Ser Ala Tyr Gly Tyr Glu Gly Asp Ala Leu Leu Ala Gln 
                645                 650                 655     


Leu Tyr Arg Asp Lys Thr Ala Ala Glu Gly Ala Val Ala Gly Val Ser 
            660                 665                 670         


Ala Val Leu Ser Thr Val Gly Ala Ala Val Ser Ile Ala Ala Ala Ala 
        675                 680                 685             


Ser Val Val Gly Ala Pro Val Ala Val Val Thr Ser Leu Leu Thr Gly 
    690                 695                 700                 


Ala Leu Asn Gly Ile Leu Arg Gly Val Gln Gln Pro Ile Ile Glu Lys 
705                 710                 715                 720 


Leu Ala Asn Asp Tyr Ala Arg Lys Ile Asp Glu Leu Gly Gly Pro Gln 
                725                 730                 735     


Ala Tyr Phe Glu Lys Asn Leu Gln Ala Arg His Glu Gln Leu Ala Asn 
            740                 745                 750         


Ser Asp Gly Leu Arg Lys Met Leu Ala Asp Leu Gln Ala Gly Trp Asn 
        755                 760                 765             


Ala Ser Ser Val Ile Gly Val Gln Thr Thr Glu Ile Ser Lys Ser Ala 
    770                 775                 780                 


Leu Glu Leu Ala Ala Ile Thr Gly Asn Ala Asp Asn Leu Lys Ser Val 
785                 790                 795                 800 


Asp Val Phe Val Asp Arg Phe Val Gln Gly Glu Arg Val Ala Gly Gln 
                805                 810                 815     


Pro Val Val Leu Asp Val Ala Ala Gly Gly Ile Asp Ile Ala Ser Arg 
            820                 825                 830         


Lys Gly Glu Arg Pro Ala Leu Thr Phe Ile Thr Pro Leu Ala Ala Pro 
        835                 840                 845             


Gly Glu Glu Gln Arg Arg Arg Thr Lys Thr Gly Lys Ser Glu Phe Thr 
    850                 855                 860                 


Thr Phe Val Glu Ile Val Gly Lys Gln Asp Arg Trp Arg Ile Arg Asp 
865                 870                 875                 880 


Gly Ala Ala Asp Thr Thr Ile Asp Leu Ala Lys Val Val Ser Gln Leu 
                885                 890                 895     


Val Asp Ala Asn Gly Val Leu Lys His Ser Ile Lys Leu Asp Val Ile 
            900                 905                 910         


Gly Gly Asp Gly Asp Asp Val Val Leu Ala Asn Ala Ser Arg Ile His 
        915                 920                 925             


Tyr Asp Gly Gly Ala Gly Thr Asn Thr Val Ser Tyr Ala Ala Leu Gly 
    930                 935                 940                 


Arg Gln Asp Ser Ile Thr Val Ser Ala Asp Gly Glu Arg Phe Asn Val 
945                 950                 955                 960 


Arg Lys Gln Leu Asn Asn Ala Asn Val Tyr Arg Glu Gly Val Ala Thr 
                965                 970                 975     


Gln Thr Thr Ala Tyr Gly Lys Arg Thr Glu Asn Val Gln Tyr Arg His 
            980                 985                 990         


Val Glu Leu Ala Arg Val Gly Gln  Leu Val Glu Val Asp  Thr Leu Glu 
        995                 1000                 1005             


His Val  Gln His Ile Ile Gly  Gly Ala Gly Asn Asp  Ser Ile Thr 
    1010                 1015                 1020             


Gly Asn  Ala His Asp Asn Phe  Leu Ala Gly Gly Ser  Gly Asp Asp 
    1025                 1030                 1035             


Arg Leu  Asp Gly Gly Ala Gly  Asn Asp Thr Leu Val  Gly Gly Glu 
    1040                 1045                 1050             


Gly Gln  Asn Thr Val Ile Gly  Gly Ala Gly Asp Asp  Val Phe Leu 
    1055                 1060                 1065             


Gln Asp  Leu Gly Val Trp Ser  Asn Gln Leu Asp Gly  Gly Ala Gly 
    1070                 1075                 1080             


Val Asp  Thr Val Lys Tyr Asn  Val His Gln Pro Ser  Glu Glu Arg 
    1085                 1090                 1095             


Leu Glu  Arg Met Gly Asp Thr  Gly Ile His Ala Asp  Leu Gln Lys 
    1100                 1105                 1110             


Gly Thr  Val Glu Lys Trp Pro  Ala Leu Asn Leu Phe  Ser Val Asp 
    1115                 1120                 1125             


His Val  Lys Asn Ile Glu Asn  Leu His Gly Ser Arg  Leu Asn Asp 
    1130                 1135                 1140             


Arg Ile  Ala Gly Asp Asp Gln  Asp Asn Glu Leu Trp  Gly His Asp 
    1145                 1150                 1155             


Gly Asn  Asp Thr Ile Arg Gly  Arg Gly Gly Asp Asp  Ile Leu Arg 
    1160                 1165                 1170             


Gly Gly  Leu Gly Leu Asp Thr  Leu Tyr Gly Glu Asp  Gly Asn Asp 
    1175                 1180                 1185             


Ile Phe  Leu Gln Asp Asp Glu  Thr Val Ser Asp Asp  Ile Asp Gly 
    1190                 1195                 1200             


Gly Ala  Gly Leu Asp Thr Val  Asp Tyr Ser Ala Met  Ile His Pro 
    1205                 1210                 1215             


Gly Arg  Ile Val Ala Pro His  Glu Tyr Gly Phe Gly  Ile Glu Ala 
    1220                 1225                 1230             


Asp Leu  Ser Arg Glu Trp Val  Arg Lys Ala Ser Ala  Leu Gly Val 
    1235                 1240                 1245             


Asp Tyr  Tyr Asp Asn Val Arg  Asn Val Glu Asn Val  Ile Gly Thr 
    1250                 1255                 1260             


Ser Met  Lys Asp Val Leu Ile  Gly Asp Ala Gln Ala  Asn Thr Leu 
    1265                 1270                 1275             


Met Gly  Gln Gly Gly Asp Asp  Thr Val Arg Gly Gly  Asp Gly Asp 
    1280                 1285                 1290             


Asp Leu  Leu Phe Gly Gly Asp  Gly Asn Asp Met Leu  Tyr Gly Asp 
    1295                 1300                 1305             


Ala Gly  Asn Asp Thr Leu Tyr  Gly Gly Leu Gly Asp  Asp Thr Leu 
    1310                 1315                 1320             


Glu Gly  Gly Ala Gly Asn Asp  Trp Phe Gly Gln Thr  Gln Ala Arg 
    1325                 1330                 1335             


Glu His  Asp Val Leu Arg Gly  Gly Asp Gly Val Asp  Thr Val Asp 
    1340                 1345                 1350             


Tyr Ser  Gln Thr Gly Ala His  Ala Gly Ile Ala Ala  Gly Arg Ile 
    1355                 1360                 1365             


Gly Leu  Gly Ile Leu Ala Asp  Leu Gly Ala Gly Arg  Val Asp Lys 
    1370                 1375                 1380             


Leu Gly  Glu Ala Gly Ser Ser  Ala Tyr Asp Thr Val  Ser Gly Ile 
    1385                 1390                 1395             


Glu Asn  Val Val Gly Thr Glu  Leu Ala Asp Arg Ile  Thr Gly Asp 
    1400                 1405                 1410             


Ala Gln  Ala Asn Val Leu Arg  Gly Ala Gly Gly Ala  Asp Val Leu 
    1415                 1420                 1425             


Ala Gly  Gly Glu Gly Asp Asp  Val Leu Leu Gly Gly  Asp Gly Asp 
    1430                 1435                 1440             


Asp Gln  Leu Ser Gly Asp Ala  Gly Arg Asp Arg Leu  Tyr Gly Glu 
    1445                 1450                 1455             


Ala Gly  Asp Asp Trp Phe Phe  Gln Asp Ala Ala Asn  Ala Gly Asn 
    1460                 1465                 1470             


Leu Leu  Asp Gly Gly Asp Gly  Arg Asp Thr Val Asp  Phe Ser Gly 
    1475                 1480                 1485             


Pro Gly  Arg Gly Leu Asp Ala  Gly Ala Lys Gly Val  Phe Leu Ser 
    1490                 1495                 1500             


Leu Gly  Lys Gly Phe Ala Ser  Leu Met Asp Glu Pro  Glu Thr Ser 
    1505                 1510                 1515             


Asn Val  Leu Arg Asn Ile Glu  Asn Ala Val Gly Ser  Ala Arg Asp 
    1520                 1525                 1530             


Asp Val  Leu Ile Gly Asp Ala  Gly Ala Asn Val Leu  Asn Gly Leu 
    1535                 1540                 1545             


Ala Gly  Asn Asp Val Leu Ser  Gly Gly Ala Gly Asp  Asp Val Leu 
    1550                 1555                 1560             


Leu Gly  Asp Glu Gly Ser Asp  Leu Leu Ser Gly Asp  Ala Gly Asn 
    1565                 1570                 1575             


Asp Asp  Leu Phe Gly Gly Gln  Gly Asp Asp Thr Tyr  Leu Phe Gly 
    1580                 1585                 1590             


Val Gly  Tyr Gly His Asp Thr  Ile Tyr Glu Ser Gly  Gly Gly His 
    1595                 1600                 1605             


Asp Thr  Ile Arg Ile Asn Ala  Gly Ala Asp Gln Leu  Trp Phe Ala 
    1610                 1615                 1620             


Arg Gln  Gly Asn Asp Leu Glu  Ile Arg Ile Leu Gly  Thr Asp Asp 
    1625                 1630                 1635             


Ala Leu  Thr Val His Asp Trp  Tyr Arg Asp Ala Asp  His Arg Val 
    1640                 1645                 1650             


Glu Ile  Ile His Ala Ala Asn  Gln Ala Val Asp Gln  Ala Gly Ile 
    1655                 1660                 1665             


Glu Lys  Leu Val Glu Ala Met  Ala Gln Tyr Pro Asp  Pro Gly Ala 
    1670                 1675                 1680             


Ala Ala  Ala Ala Pro Pro Ala  Ala Arg Val Pro Asp  Thr Leu Met 
    1685                 1690                 1695             


Gln Ser  Leu Ala Val Asn Trp  Arg 
    1700                 1705     


<210>  2
<211>  697
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adenylate cyclase fragment without His tag

<400>  2

Thr Glu Asn Val Gln Tyr Arg His Val Glu Leu Ala Arg Val Gly Gln 
1               5                   10                  15      


Leu Val Glu Val Asp Thr Leu Glu His Val Gln His Ile Ile Gly Gly 
            20                  25                  30          


Ala Gly Asn Asp Ser Ile Thr Gly Asn Ala His Asp Asn Phe Leu Ala 
        35                  40                  45              


Gly Gly Ser Gly Asp Asp Arg Leu Asp Gly Gly Ala Gly Asn Asp Thr 
    50                  55                  60                  


Leu Val Gly Gly Glu Gly Gln Asn Thr Val Ile Gly Gly Ala Gly Asp 
65                  70                  75                  80  


Asp Val Phe Leu Gln Asp Leu Gly Val Trp Ser Asn Gln Leu Asp Gly 
                85                  90                  95      


Gly Ala Gly Val Asp Thr Val Lys Tyr Asn Val His Gln Pro Ser Glu 
            100                 105                 110         


Glu Arg Leu Glu Arg Met Gly Asp Thr Gly Ile His Ala Asp Leu Gln 
        115                 120                 125             


Lys Gly Thr Val Glu Lys Trp Pro Ala Leu Asn Leu Phe Ser Val Asp 
    130                 135                 140                 


His Val Lys Asn Ile Glu Asn Leu His Gly Ser Arg Leu Asn Asp Arg 
145                 150                 155                 160 


Ile Ala Gly Asp Asp Gln Asp Asn Glu Leu Trp Gly His Asp Gly Asn 
                165                 170                 175     


Asp Thr Ile Arg Gly Arg Gly Gly Asp Asp Ile Leu Arg Gly Gly Leu 
            180                 185                 190         


Gly Leu Asp Thr Leu Tyr Gly Glu Asp Gly Asn Asp Ile Phe Leu Gln 
        195                 200                 205             


Asp Asp Glu Thr Val Ser Asp Asp Ile Asp Gly Gly Ala Gly Leu Asp 
    210                 215                 220                 


Thr Val Asp Tyr Ser Ala Met Ile His Pro Gly Arg Ile Val Ala Pro 
225                 230                 235                 240 


His Glu Tyr Gly Phe Gly Ile Glu Ala Asp Leu Ser Arg Glu Trp Val 
                245                 250                 255     


Arg Lys Ala Ser Ala Leu Gly Val Asp Tyr Tyr Asp Asn Val Arg Asn 
            260                 265                 270         


Val Glu Asn Val Ile Gly Thr Ser Met Lys Asp Val Leu Ile Gly Asp 
        275                 280                 285             


Ala Gln Ala Asn Thr Leu Met Gly Gln Gly Gly Asp Asp Thr Val Arg 
    290                 295                 300                 


Gly Gly Asp Gly Asp Asp Leu Leu Phe Gly Gly Asp Gly Asn Asp Met 
305                 310                 315                 320 


Leu Tyr Gly Asp Ala Gly Asn Asp Thr Leu Tyr Gly Gly Leu Gly Asp 
                325                 330                 335     


Asp Thr Leu Glu Gly Gly Ala Gly Asn Asp Trp Phe Gly Gln Thr Gln 
            340                 345                 350         


Ala Arg Glu His Asp Val Leu Arg Gly Gly Asp Gly Val Asp Thr Val 
        355                 360                 365             


Asp Tyr Ser Gln Thr Gly Ala His Ala Gly Ile Ala Ala Gly Arg Ile 
    370                 375                 380                 


Gly Leu Gly Ile Leu Ala Asp Leu Gly Ala Gly Arg Val Asp Lys Leu 
385                 390                 395                 400 


Gly Glu Ala Gly Ser Ser Ala Tyr Asp Thr Val Ser Gly Ile Glu Asn 
                405                 410                 415     


Val Val Gly Thr Glu Leu Ala Asp Arg Ile Thr Gly Asp Ala Gln Ala 
            420                 425                 430         


Asn Val Leu Arg Gly Ala Gly Gly Ala Asp Val Leu Ala Gly Gly Glu 
        435                 440                 445             


Gly Asp Asp Val Leu Leu Gly Gly Asp Gly Asp Asp Gln Leu Ser Gly 
    450                 455                 460                 


Asp Ala Gly Arg Asp Arg Leu Tyr Gly Glu Ala Gly Asp Asp Trp Phe 
465                 470                 475                 480 


Phe Gln Asp Ala Ala Asn Ala Gly Asn Leu Leu Asp Gly Gly Asp Gly 
                485                 490                 495     


Arg Asp Thr Val Asp Phe Ser Gly Pro Gly Arg Gly Leu Asp Ala Gly 
            500                 505                 510         


Ala Lys Gly Val Phe Leu Ser Leu Gly Lys Gly Phe Ala Ser Leu Met 
        515                 520                 525             


Asp Glu Pro Glu Thr Ser Asn Val Leu Arg Asn Ile Glu Asn Ala Val 
    530                 535                 540                 


Gly Ser Ala Arg Asp Asp Val Leu Ile Gly Asp Ala Gly Ala Asn Val 
545                 550                 555                 560 


Leu Asn Gly Leu Ala Gly Asn Asp Val Leu Ser Gly Gly Ala Gly Asp 
                565                 570                 575     


Asp Val Leu Leu Gly Asp Glu Gly Ser Asp Leu Leu Ser Gly Asp Ala 
            580                 585                 590         


Gly Asn Asp Asp Leu Phe Gly Gly Gln Gly Asp Asp Thr Tyr Leu Phe 
        595                 600                 605             


Gly Val Gly Tyr Gly His Asp Thr Ile Tyr Glu Ser Gly Gly Gly His 
    610                 615                 620                 


Asp Thr Ile Arg Ile Asn Ala Gly Ala Asp Gln Leu Trp Phe Ala Arg 
625                 630                 635                 640 


Gln Gly Asn Asp Leu Glu Ile Arg Ile Leu Gly Thr Asp Asp Ala Leu 
                645                 650                 655     


Thr Val His Asp Trp Tyr Arg Asp Ala Asp His Arg Val Glu Ile Ile 
            660                 665                 670         


His Ala Ala Asn Gln Ala Val Asp Gln Ala Gly Ile Glu Lys Leu Val 
        675                 680                 685             


Glu Ala Met Ala Gln Tyr Pro Asp Pro 
    690                 695         


<210>  3
<211>  698
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adenylate cyclase fragment without His tag

<400>  3

Thr Glu Asn Val Gln Tyr Arg His Val Glu Leu Ala Arg Val Gly Gln 
1               5                   10                  15      


Leu Val Glu Val Asp Thr Leu Glu His Val Gln His Ile Ile Gly Gly 
            20                  25                  30          


Ala Gly Asn Asp Ser Ile Thr Gly Asn Ala His Asp Asn Phe Leu Ala 
        35                  40                  45              


Gly Gly Ser Gly Asp Asp Arg Leu Asp Gly Gly Ala Gly Asn Asp Thr 
    50                  55                  60                  


Leu Val Gly Gly Glu Gly Gln Asn Thr Val Ile Gly Gly Ala Gly Asp 
65                  70                  75                  80  


Asp Val Phe Leu Gln Asp Leu Gly Val Trp Ser Asn Gln Leu Asp Gly 
                85                  90                  95      


Gly Ala Gly Val Asp Thr Val Lys Tyr Asn Val His Gln Pro Ser Glu 
            100                 105                 110         


Glu Arg Leu Glu Arg Met Gly Asp Thr Gly Ile His Ala Asp Leu Gln 
        115                 120                 125             


Lys Gly Thr Val Glu Lys Trp Pro Ala Leu Asn Leu Phe Ser Val Asp 
    130                 135                 140                 


His Val Lys Asn Ile Glu Asn Leu His Gly Ser Arg Leu Asn Asp Arg 
145                 150                 155                 160 


Ile Ala Gly Asp Asp Gln Asp Asn Glu Leu Trp Gly His Asp Gly Asn 
                165                 170                 175     


Asp Thr Ile Arg Gly Arg Gly Gly Asp Asp Ile Leu Arg Gly Gly Leu 
            180                 185                 190         


Gly Leu Asp Thr Leu Tyr Gly Glu Asp Gly Asn Asp Ile Phe Leu Gln 
        195                 200                 205             


Asp Asp Glu Thr Val Ser Asp Asp Ile Asp Gly Gly Ala Gly Leu Asp 
    210                 215                 220                 


Thr Val Asp Tyr Ser Ala Met Ile His Pro Gly Arg Ile Val Ala Pro 
225                 230                 235                 240 


His Glu Tyr Gly Phe Gly Ile Glu Ala Asp Leu Ser Arg Glu Trp Val 
                245                 250                 255     


Arg Lys Ala Ser Ala Leu Gly Val Asp Tyr Tyr Asp Asn Val Arg Asn 
            260                 265                 270         


Val Glu Asn Val Ile Gly Thr Ser Met Lys Asp Val Leu Ile Gly Asp 
        275                 280                 285             


Ala Gln Ala Asn Thr Leu Met Gly Gln Gly Gly Asp Asp Thr Val Arg 
    290                 295                 300                 


Gly Gly Asp Gly Asp Asp Leu Leu Phe Gly Gly Asp Gly Asn Asp Met 
305                 310                 315                 320 


Leu Tyr Gly Asp Ala Gly Asn Asp Thr Leu Tyr Gly Gly Leu Gly Asp 
                325                 330                 335     


Asp Thr Leu Glu Gly Gly Ala Gly Asn Asp Trp Phe Gly Gln Thr Gln 
            340                 345                 350         


Ala Arg Glu His Asp Val Leu Arg Gly Gly Asp Gly Val Asp Thr Val 
        355                 360                 365             


Asp Tyr Ser Gln Thr Gly Ala His Ala Gly Ile Ala Ala Gly Arg Ile 
    370                 375                 380                 


Gly Leu Gly Ile Leu Ala Asp Leu Gly Ala Gly Arg Val Asp Lys Leu 
385                 390                 395                 400 


Gly Glu Ala Gly Ser Ser Ala Tyr Asp Thr Val Ser Gly Ile Glu Asn 
                405                 410                 415     


Val Val Gly Thr Glu Leu Ala Asp Arg Ile Thr Gly Asp Ala Gln Ala 
            420                 425                 430         


Asn Val Leu Arg Gly Ala Gly Gly Ala Asp Val Leu Ala Gly Gly Glu 
        435                 440                 445             


Gly Asp Asp Val Leu Leu Gly Gly Asp Gly Asp Asp Gln Leu Ser Gly 
    450                 455                 460                 


Asp Ala Gly Arg Asp Arg Leu Tyr Gly Glu Ala Gly Asp Asp Trp Phe 
465                 470                 475                 480 


Phe Gln Asp Ala Ala Asn Ala Gly Asn Leu Leu Asp Gly Gly Asp Gly 
                485                 490                 495     


Arg Asp Thr Val Asp Phe Ser Gly Pro Gly Arg Gly Leu Asp Ala Gly 
            500                 505                 510         


Ala Lys Gly Val Phe Leu Ser Leu Gly Lys Gly Phe Ala Ser Leu Met 
        515                 520                 525             


Asp Glu Pro Glu Thr Ser Asn Val Leu Arg Asn Ile Glu Asn Ala Val 
    530                 535                 540                 


Gly Ser Ala Arg Asp Asp Val Leu Ile Gly Asp Ala Gly Ala Asn Val 
545                 550                 555                 560 


Leu Asn Gly Leu Ala Gly Asn Asp Val Leu Ser Gly Gly Ala Gly Asp 
                565                 570                 575     


Asp Val Leu Leu Gly Asp Glu Gly Ser Asp Leu Leu Ser Gly Asp Ala 
            580                 585                 590         


Gly Asn Asp Asp Leu Phe Gly Gly Gln Gly Asp Asp Thr Tyr Leu Phe 
        595                 600                 605             


Gly Val Gly Tyr Gly His Asp Thr Ile Tyr Glu Ser Gly Gly Gly His 
    610                 615                 620                 


Asp Thr Ile Arg Ile Asn Ala Gly Ala Asp Gln Leu Trp Phe Ala Arg 
625                 630                 635                 640 


Gln Gly Asn Asp Leu Glu Ile Arg Ile Leu Gly Thr Asp Asp Ala Leu 
                645                 650                 655     


Thr Val His Asp Trp Tyr Arg Asp Ala Asp His Arg Val Glu Ile Ile 
            660                 665                 670         


His Ala Ala Asn Gln Ala Val Asp Gln Ala Gly Ile Glu Lys Leu Val 
        675                 680                 685             


Glu Ala Met Ala Gln Tyr Pro Asp Pro Gly 
    690                 695             


<210>  4
<211>  699
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adenylate cyclase fragment without His tag

<400>  4

Thr Glu Asn Val Gln Tyr Arg His Val Glu Leu Ala Arg Val Gly Gln 
1               5                   10                  15      


Leu Val Glu Val Asp Thr Leu Glu His Val Gln His Ile Ile Gly Gly 
            20                  25                  30          


Ala Gly Asn Asp Ser Ile Thr Gly Asn Ala His Asp Asn Phe Leu Ala 
        35                  40                  45              


Gly Gly Ser Gly Asp Asp Arg Leu Asp Gly Gly Ala Gly Asn Asp Thr 
    50                  55                  60                  


Leu Val Gly Gly Glu Gly Gln Asn Thr Val Ile Gly Gly Ala Gly Asp 
65                  70                  75                  80  


Asp Val Phe Leu Gln Asp Leu Gly Val Trp Ser Asn Gln Leu Asp Gly 
                85                  90                  95      


Gly Ala Gly Val Asp Thr Val Lys Tyr Asn Val His Gln Pro Ser Glu 
            100                 105                 110         


Glu Arg Leu Glu Arg Met Gly Asp Thr Gly Ile His Ala Asp Leu Gln 
        115                 120                 125             


Lys Gly Thr Val Glu Lys Trp Pro Ala Leu Asn Leu Phe Ser Val Asp 
    130                 135                 140                 


His Val Lys Asn Ile Glu Asn Leu His Gly Ser Arg Leu Asn Asp Arg 
145                 150                 155                 160 


Ile Ala Gly Asp Asp Gln Asp Asn Glu Leu Trp Gly His Asp Gly Asn 
                165                 170                 175     


Asp Thr Ile Arg Gly Arg Gly Gly Asp Asp Ile Leu Arg Gly Gly Leu 
            180                 185                 190         


Gly Leu Asp Thr Leu Tyr Gly Glu Asp Gly Asn Asp Ile Phe Leu Gln 
        195                 200                 205             


Asp Asp Glu Thr Val Ser Asp Asp Ile Asp Gly Gly Ala Gly Leu Asp 
    210                 215                 220                 


Thr Val Asp Tyr Ser Ala Met Ile His Pro Gly Arg Ile Val Ala Pro 
225                 230                 235                 240 


His Glu Tyr Gly Phe Gly Ile Glu Ala Asp Leu Ser Arg Glu Trp Val 
                245                 250                 255     


Arg Lys Ala Ser Ala Leu Gly Val Asp Tyr Tyr Asp Asn Val Arg Asn 
            260                 265                 270         


Val Glu Asn Val Ile Gly Thr Ser Met Lys Asp Val Leu Ile Gly Asp 
        275                 280                 285             


Ala Gln Ala Asn Thr Leu Met Gly Gln Gly Gly Asp Asp Thr Val Arg 
    290                 295                 300                 


Gly Gly Asp Gly Asp Asp Leu Leu Phe Gly Gly Asp Gly Asn Asp Met 
305                 310                 315                 320 


Leu Tyr Gly Asp Ala Gly Asn Asp Thr Leu Tyr Gly Gly Leu Gly Asp 
                325                 330                 335     


Asp Thr Leu Glu Gly Gly Ala Gly Asn Asp Trp Phe Gly Gln Thr Gln 
            340                 345                 350         


Ala Arg Glu His Asp Val Leu Arg Gly Gly Asp Gly Val Asp Thr Val 
        355                 360                 365             


Asp Tyr Ser Gln Thr Gly Ala His Ala Gly Ile Ala Ala Gly Arg Ile 
    370                 375                 380                 


Gly Leu Gly Ile Leu Ala Asp Leu Gly Ala Gly Arg Val Asp Lys Leu 
385                 390                 395                 400 


Gly Glu Ala Gly Ser Ser Ala Tyr Asp Thr Val Ser Gly Ile Glu Asn 
                405                 410                 415     


Val Val Gly Thr Glu Leu Ala Asp Arg Ile Thr Gly Asp Ala Gln Ala 
            420                 425                 430         


Asn Val Leu Arg Gly Ala Gly Gly Ala Asp Val Leu Ala Gly Gly Glu 
        435                 440                 445             


Gly Asp Asp Val Leu Leu Gly Gly Asp Gly Asp Asp Gln Leu Ser Gly 
    450                 455                 460                 


Asp Ala Gly Arg Asp Arg Leu Tyr Gly Glu Ala Gly Asp Asp Trp Phe 
465                 470                 475                 480 


Phe Gln Asp Ala Ala Asn Ala Gly Asn Leu Leu Asp Gly Gly Asp Gly 
                485                 490                 495     


Arg Asp Thr Val Asp Phe Ser Gly Pro Gly Arg Gly Leu Asp Ala Gly 
            500                 505                 510         


Ala Lys Gly Val Phe Leu Ser Leu Gly Lys Gly Phe Ala Ser Leu Met 
        515                 520                 525             


Asp Glu Pro Glu Thr Ser Asn Val Leu Arg Asn Ile Glu Asn Ala Val 
    530                 535                 540                 


Gly Ser Ala Arg Asp Asp Val Leu Ile Gly Asp Ala Gly Ala Asn Val 
545                 550                 555                 560 


Leu Asn Gly Leu Ala Gly Asn Asp Val Leu Ser Gly Gly Ala Gly Asp 
                565                 570                 575     


Asp Val Leu Leu Gly Asp Glu Gly Ser Asp Leu Leu Ser Gly Asp Ala 
            580                 585                 590         


Gly Asn Asp Asp Leu Phe Gly Gly Gln Gly Asp Asp Thr Tyr Leu Phe 
        595                 600                 605             


Gly Val Gly Tyr Gly His Asp Thr Ile Tyr Glu Ser Gly Gly Gly His 
    610                 615                 620                 


Asp Thr Ile Arg Ile Asn Ala Gly Ala Asp Gln Leu Trp Phe Ala Arg 
625                 630                 635                 640 


Gln Gly Asn Asp Leu Glu Ile Arg Ile Leu Gly Thr Asp Asp Ala Leu 
                645                 650                 655     


Thr Val His Asp Trp Tyr Arg Asp Ala Asp His Arg Val Glu Ile Ile 
            660                 665                 670         


His Ala Ala Asn Gln Ala Val Asp Gln Ala Gly Ile Glu Lys Leu Val 
        675                 680                 685             


Glu Ala Met Ala Gln Tyr Pro Asp Pro Gly Gly 
    690                 695                 


<210>  5
<211>  703
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adenylate cyclase plus His tag 1

<400>  5

Thr Glu Asn Val Gln Tyr Arg His Val Glu Leu Ala Arg Val Gly Gln 
1               5                   10                  15      


Leu Val Glu Val Asp Thr Leu Glu His Val Gln His Ile Ile Gly Gly 
            20                  25                  30          


Ala Gly Asn Asp Ser Ile Thr Gly Asn Ala His Asp Asn Phe Leu Ala 
        35                  40                  45              


Gly Gly Ser Gly Asp Asp Arg Leu Asp Gly Gly Ala Gly Asn Asp Thr 
    50                  55                  60                  


Leu Val Gly Gly Glu Gly Gln Asn Thr Val Ile Gly Gly Ala Gly Asp 
65                  70                  75                  80  


Asp Val Phe Leu Gln Asp Leu Gly Val Trp Ser Asn Gln Leu Asp Gly 
                85                  90                  95      


Gly Ala Gly Val Asp Thr Val Lys Tyr Asn Val His Gln Pro Ser Glu 
            100                 105                 110         


Glu Arg Leu Glu Arg Met Gly Asp Thr Gly Ile His Ala Asp Leu Gln 
        115                 120                 125             


Lys Gly Thr Val Glu Lys Trp Pro Ala Leu Asn Leu Phe Ser Val Asp 
    130                 135                 140                 


His Val Lys Asn Ile Glu Asn Leu His Gly Ser Arg Leu Asn Asp Arg 
145                 150                 155                 160 


Ile Ala Gly Asp Asp Gln Asp Asn Glu Leu Trp Gly His Asp Gly Asn 
                165                 170                 175     


Asp Thr Ile Arg Gly Arg Gly Gly Asp Asp Ile Leu Arg Gly Gly Leu 
            180                 185                 190         


Gly Leu Asp Thr Leu Tyr Gly Glu Asp Gly Asn Asp Ile Phe Leu Gln 
        195                 200                 205             


Asp Asp Glu Thr Val Ser Asp Asp Ile Asp Gly Gly Ala Gly Leu Asp 
    210                 215                 220                 


Thr Val Asp Tyr Ser Ala Met Ile His Pro Gly Arg Ile Val Ala Pro 
225                 230                 235                 240 


His Glu Tyr Gly Phe Gly Ile Glu Ala Asp Leu Ser Arg Glu Trp Val 
                245                 250                 255     


Arg Lys Ala Ser Ala Leu Gly Val Asp Tyr Tyr Asp Asn Val Arg Asn 
            260                 265                 270         


Val Glu Asn Val Ile Gly Thr Ser Met Lys Asp Val Leu Ile Gly Asp 
        275                 280                 285             


Ala Gln Ala Asn Thr Leu Met Gly Gln Gly Gly Asp Asp Thr Val Arg 
    290                 295                 300                 


Gly Gly Asp Gly Asp Asp Leu Leu Phe Gly Gly Asp Gly Asn Asp Met 
305                 310                 315                 320 


Leu Tyr Gly Asp Ala Gly Asn Asp Thr Leu Tyr Gly Gly Leu Gly Asp 
                325                 330                 335     


Asp Thr Leu Glu Gly Gly Ala Gly Asn Asp Trp Phe Gly Gln Thr Gln 
            340                 345                 350         


Ala Arg Glu His Asp Val Leu Arg Gly Gly Asp Gly Val Asp Thr Val 
        355                 360                 365             


Asp Tyr Ser Gln Thr Gly Ala His Ala Gly Ile Ala Ala Gly Arg Ile 
    370                 375                 380                 


Gly Leu Gly Ile Leu Ala Asp Leu Gly Ala Gly Arg Val Asp Lys Leu 
385                 390                 395                 400 


Gly Glu Ala Gly Ser Ser Ala Tyr Asp Thr Val Ser Gly Ile Glu Asn 
                405                 410                 415     


Val Val Gly Thr Glu Leu Ala Asp Arg Ile Thr Gly Asp Ala Gln Ala 
            420                 425                 430         


Asn Val Leu Arg Gly Ala Gly Gly Ala Asp Val Leu Ala Gly Gly Glu 
        435                 440                 445             


Gly Asp Asp Val Leu Leu Gly Gly Asp Gly Asp Asp Gln Leu Ser Gly 
    450                 455                 460                 


Asp Ala Gly Arg Asp Arg Leu Tyr Gly Glu Ala Gly Asp Asp Trp Phe 
465                 470                 475                 480 


Phe Gln Asp Ala Ala Asn Ala Gly Asn Leu Leu Asp Gly Gly Asp Gly 
                485                 490                 495     


Arg Asp Thr Val Asp Phe Ser Gly Pro Gly Arg Gly Leu Asp Ala Gly 
            500                 505                 510         


Ala Lys Gly Val Phe Leu Ser Leu Gly Lys Gly Phe Ala Ser Leu Met 
        515                 520                 525             


Asp Glu Pro Glu Thr Ser Asn Val Leu Arg Asn Ile Glu Asn Ala Val 
    530                 535                 540                 


Gly Ser Ala Arg Asp Asp Val Leu Ile Gly Asp Ala Gly Ala Asn Val 
545                 550                 555                 560 


Leu Asn Gly Leu Ala Gly Asn Asp Val Leu Ser Gly Gly Ala Gly Asp 
                565                 570                 575     


Asp Val Leu Leu Gly Asp Glu Gly Ser Asp Leu Leu Ser Gly Asp Ala 
            580                 585                 590         


Gly Asn Asp Asp Leu Phe Gly Gly Gln Gly Asp Asp Thr Tyr Leu Phe 
        595                 600                 605             


Gly Val Gly Tyr Gly His Asp Thr Ile Tyr Glu Ser Gly Gly Gly His 
    610                 615                 620                 


Asp Thr Ile Arg Ile Asn Ala Gly Ala Asp Gln Leu Trp Phe Ala Arg 
625                 630                 635                 640 


Gln Gly Asn Asp Leu Glu Ile Arg Ile Leu Gly Thr Asp Asp Ala Leu 
                645                 650                 655     


Thr Val His Asp Trp Tyr Arg Asp Ala Asp His Arg Val Glu Ile Ile 
            660                 665                 670         


His Ala Ala Asn Gln Ala Val Asp Gln Ala Gly Ile Glu Lys Leu Val 
        675                 680                 685             


Glu Ala Met Ala Gln Tyr Pro Asp Pro His His His His His His 
    690                 695                 700             


<210>  6
<211>  704
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adenylate cyclase plus His tag 2

<400>  6

Thr Glu Asn Val Gln Tyr Arg His Val Glu Leu Ala Arg Val Gly Gln 
1               5                   10                  15      


Leu Val Glu Val Asp Thr Leu Glu His Val Gln His Ile Ile Gly Gly 
            20                  25                  30          


Ala Gly Asn Asp Ser Ile Thr Gly Asn Ala His Asp Asn Phe Leu Ala 
        35                  40                  45              


Gly Gly Ser Gly Asp Asp Arg Leu Asp Gly Gly Ala Gly Asn Asp Thr 
    50                  55                  60                  


Leu Val Gly Gly Glu Gly Gln Asn Thr Val Ile Gly Gly Ala Gly Asp 
65                  70                  75                  80  


Asp Val Phe Leu Gln Asp Leu Gly Val Trp Ser Asn Gln Leu Asp Gly 
                85                  90                  95      


Gly Ala Gly Val Asp Thr Val Lys Tyr Asn Val His Gln Pro Ser Glu 
            100                 105                 110         


Glu Arg Leu Glu Arg Met Gly Asp Thr Gly Ile His Ala Asp Leu Gln 
        115                 120                 125             


Lys Gly Thr Val Glu Lys Trp Pro Ala Leu Asn Leu Phe Ser Val Asp 
    130                 135                 140                 


His Val Lys Asn Ile Glu Asn Leu His Gly Ser Arg Leu Asn Asp Arg 
145                 150                 155                 160 


Ile Ala Gly Asp Asp Gln Asp Asn Glu Leu Trp Gly His Asp Gly Asn 
                165                 170                 175     


Asp Thr Ile Arg Gly Arg Gly Gly Asp Asp Ile Leu Arg Gly Gly Leu 
            180                 185                 190         


Gly Leu Asp Thr Leu Tyr Gly Glu Asp Gly Asn Asp Ile Phe Leu Gln 
        195                 200                 205             


Asp Asp Glu Thr Val Ser Asp Asp Ile Asp Gly Gly Ala Gly Leu Asp 
    210                 215                 220                 


Thr Val Asp Tyr Ser Ala Met Ile His Pro Gly Arg Ile Val Ala Pro 
225                 230                 235                 240 


His Glu Tyr Gly Phe Gly Ile Glu Ala Asp Leu Ser Arg Glu Trp Val 
                245                 250                 255     


Arg Lys Ala Ser Ala Leu Gly Val Asp Tyr Tyr Asp Asn Val Arg Asn 
            260                 265                 270         


Val Glu Asn Val Ile Gly Thr Ser Met Lys Asp Val Leu Ile Gly Asp 
        275                 280                 285             


Ala Gln Ala Asn Thr Leu Met Gly Gln Gly Gly Asp Asp Thr Val Arg 
    290                 295                 300                 


Gly Gly Asp Gly Asp Asp Leu Leu Phe Gly Gly Asp Gly Asn Asp Met 
305                 310                 315                 320 


Leu Tyr Gly Asp Ala Gly Asn Asp Thr Leu Tyr Gly Gly Leu Gly Asp 
                325                 330                 335     


Asp Thr Leu Glu Gly Gly Ala Gly Asn Asp Trp Phe Gly Gln Thr Gln 
            340                 345                 350         


Ala Arg Glu His Asp Val Leu Arg Gly Gly Asp Gly Val Asp Thr Val 
        355                 360                 365             


Asp Tyr Ser Gln Thr Gly Ala His Ala Gly Ile Ala Ala Gly Arg Ile 
    370                 375                 380                 


Gly Leu Gly Ile Leu Ala Asp Leu Gly Ala Gly Arg Val Asp Lys Leu 
385                 390                 395                 400 


Gly Glu Ala Gly Ser Ser Ala Tyr Asp Thr Val Ser Gly Ile Glu Asn 
                405                 410                 415     


Val Val Gly Thr Glu Leu Ala Asp Arg Ile Thr Gly Asp Ala Gln Ala 
            420                 425                 430         


Asn Val Leu Arg Gly Ala Gly Gly Ala Asp Val Leu Ala Gly Gly Glu 
        435                 440                 445             


Gly Asp Asp Val Leu Leu Gly Gly Asp Gly Asp Asp Gln Leu Ser Gly 
    450                 455                 460                 


Asp Ala Gly Arg Asp Arg Leu Tyr Gly Glu Ala Gly Asp Asp Trp Phe 
465                 470                 475                 480 


Phe Gln Asp Ala Ala Asn Ala Gly Asn Leu Leu Asp Gly Gly Asp Gly 
                485                 490                 495     


Arg Asp Thr Val Asp Phe Ser Gly Pro Gly Arg Gly Leu Asp Ala Gly 
            500                 505                 510         


Ala Lys Gly Val Phe Leu Ser Leu Gly Lys Gly Phe Ala Ser Leu Met 
        515                 520                 525             


Asp Glu Pro Glu Thr Ser Asn Val Leu Arg Asn Ile Glu Asn Ala Val 
    530                 535                 540                 


Gly Ser Ala Arg Asp Asp Val Leu Ile Gly Asp Ala Gly Ala Asn Val 
545                 550                 555                 560 


Leu Asn Gly Leu Ala Gly Asn Asp Val Leu Ser Gly Gly Ala Gly Asp 
                565                 570                 575     


Asp Val Leu Leu Gly Asp Glu Gly Ser Asp Leu Leu Ser Gly Asp Ala 
            580                 585                 590         


Gly Asn Asp Asp Leu Phe Gly Gly Gln Gly Asp Asp Thr Tyr Leu Phe 
        595                 600                 605             


Gly Val Gly Tyr Gly His Asp Thr Ile Tyr Glu Ser Gly Gly Gly His 
    610                 615                 620                 


Asp Thr Ile Arg Ile Asn Ala Gly Ala Asp Gln Leu Trp Phe Ala Arg 
625                 630                 635                 640 


Gln Gly Asn Asp Leu Glu Ile Arg Ile Leu Gly Thr Asp Asp Ala Leu 
                645                 650                 655     


Thr Val His Asp Trp Tyr Arg Asp Ala Asp His Arg Val Glu Ile Ile 
            660                 665                 670         


His Ala Ala Asn Gln Ala Val Asp Gln Ala Gly Ile Glu Lys Leu Val 
        675                 680                 685             


Glu Ala Met Ala Gln Tyr Pro Asp Pro Gly His His His His His His 
    690                 695                 700                 


<210>  7
<211>  705
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adenylate cyclase plus His tag 3

<400>  7

Thr Glu Asn Val Gln Tyr Arg His Val Glu Leu Ala Arg Val Gly Gln 
1               5                   10                  15      


Leu Val Glu Val Asp Thr Leu Glu His Val Gln His Ile Ile Gly Gly 
            20                  25                  30          


Ala Gly Asn Asp Ser Ile Thr Gly Asn Ala His Asp Asn Phe Leu Ala 
        35                  40                  45              


Gly Gly Ser Gly Asp Asp Arg Leu Asp Gly Gly Ala Gly Asn Asp Thr 
    50                  55                  60                  


Leu Val Gly Gly Glu Gly Gln Asn Thr Val Ile Gly Gly Ala Gly Asp 
65                  70                  75                  80  


Asp Val Phe Leu Gln Asp Leu Gly Val Trp Ser Asn Gln Leu Asp Gly 
                85                  90                  95      


Gly Ala Gly Val Asp Thr Val Lys Tyr Asn Val His Gln Pro Ser Glu 
            100                 105                 110         


Glu Arg Leu Glu Arg Met Gly Asp Thr Gly Ile His Ala Asp Leu Gln 
        115                 120                 125             


Lys Gly Thr Val Glu Lys Trp Pro Ala Leu Asn Leu Phe Ser Val Asp 
    130                 135                 140                 


His Val Lys Asn Ile Glu Asn Leu His Gly Ser Arg Leu Asn Asp Arg 
145                 150                 155                 160 


Ile Ala Gly Asp Asp Gln Asp Asn Glu Leu Trp Gly His Asp Gly Asn 
                165                 170                 175     


Asp Thr Ile Arg Gly Arg Gly Gly Asp Asp Ile Leu Arg Gly Gly Leu 
            180                 185                 190         


Gly Leu Asp Thr Leu Tyr Gly Glu Asp Gly Asn Asp Ile Phe Leu Gln 
        195                 200                 205             


Asp Asp Glu Thr Val Ser Asp Asp Ile Asp Gly Gly Ala Gly Leu Asp 
    210                 215                 220                 


Thr Val Asp Tyr Ser Ala Met Ile His Pro Gly Arg Ile Val Ala Pro 
225                 230                 235                 240 


His Glu Tyr Gly Phe Gly Ile Glu Ala Asp Leu Ser Arg Glu Trp Val 
                245                 250                 255     


Arg Lys Ala Ser Ala Leu Gly Val Asp Tyr Tyr Asp Asn Val Arg Asn 
            260                 265                 270         


Val Glu Asn Val Ile Gly Thr Ser Met Lys Asp Val Leu Ile Gly Asp 
        275                 280                 285             


Ala Gln Ala Asn Thr Leu Met Gly Gln Gly Gly Asp Asp Thr Val Arg 
    290                 295                 300                 


Gly Gly Asp Gly Asp Asp Leu Leu Phe Gly Gly Asp Gly Asn Asp Met 
305                 310                 315                 320 


Leu Tyr Gly Asp Ala Gly Asn Asp Thr Leu Tyr Gly Gly Leu Gly Asp 
                325                 330                 335     


Asp Thr Leu Glu Gly Gly Ala Gly Asn Asp Trp Phe Gly Gln Thr Gln 
            340                 345                 350         


Ala Arg Glu His Asp Val Leu Arg Gly Gly Asp Gly Val Asp Thr Val 
        355                 360                 365             


Asp Tyr Ser Gln Thr Gly Ala His Ala Gly Ile Ala Ala Gly Arg Ile 
    370                 375                 380                 


Gly Leu Gly Ile Leu Ala Asp Leu Gly Ala Gly Arg Val Asp Lys Leu 
385                 390                 395                 400 


Gly Glu Ala Gly Ser Ser Ala Tyr Asp Thr Val Ser Gly Ile Glu Asn 
                405                 410                 415     


Val Val Gly Thr Glu Leu Ala Asp Arg Ile Thr Gly Asp Ala Gln Ala 
            420                 425                 430         


Asn Val Leu Arg Gly Ala Gly Gly Ala Asp Val Leu Ala Gly Gly Glu 
        435                 440                 445             


Gly Asp Asp Val Leu Leu Gly Gly Asp Gly Asp Asp Gln Leu Ser Gly 
    450                 455                 460                 


Asp Ala Gly Arg Asp Arg Leu Tyr Gly Glu Ala Gly Asp Asp Trp Phe 
465                 470                 475                 480 


Phe Gln Asp Ala Ala Asn Ala Gly Asn Leu Leu Asp Gly Gly Asp Gly 
                485                 490                 495     


Arg Asp Thr Val Asp Phe Ser Gly Pro Gly Arg Gly Leu Asp Ala Gly 
            500                 505                 510         


Ala Lys Gly Val Phe Leu Ser Leu Gly Lys Gly Phe Ala Ser Leu Met 
        515                 520                 525             


Asp Glu Pro Glu Thr Ser Asn Val Leu Arg Asn Ile Glu Asn Ala Val 
    530                 535                 540                 


Gly Ser Ala Arg Asp Asp Val Leu Ile Gly Asp Ala Gly Ala Asn Val 
545                 550                 555                 560 


Leu Asn Gly Leu Ala Gly Asn Asp Val Leu Ser Gly Gly Ala Gly Asp 
                565                 570                 575     


Asp Val Leu Leu Gly Asp Glu Gly Ser Asp Leu Leu Ser Gly Asp Ala 
            580                 585                 590         


Gly Asn Asp Asp Leu Phe Gly Gly Gln Gly Asp Asp Thr Tyr Leu Phe 
        595                 600                 605             


Gly Val Gly Tyr Gly His Asp Thr Ile Tyr Glu Ser Gly Gly Gly His 
    610                 615                 620                 


Asp Thr Ile Arg Ile Asn Ala Gly Ala Asp Gln Leu Trp Phe Ala Arg 
625                 630                 635                 640 


Gln Gly Asn Asp Leu Glu Ile Arg Ile Leu Gly Thr Asp Asp Ala Leu 
                645                 650                 655     


Thr Val His Asp Trp Tyr Arg Asp Ala Asp His Arg Val Glu Ile Ile 
            660                 665                 670         


His Ala Ala Asn Gln Ala Val Asp Gln Ala Gly Ile Glu Lys Leu Val 
        675                 680                 685             


Glu Ala Met Ala Gln Tyr Pro Asp Pro Gly Gly His His His His His 
    690                 695                 700                 


His 
705 


<210>  8
<211>  726
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragment plus NspA signal peptide

<400>  8

Met Lys Lys Ala Leu Ala Thr Leu Ile Ala Leu Ala Leu Pro Ala Ala 
1               5                   10                  15      


Ala Leu Ala Glu Gly Thr Glu Asn Val Gln Tyr Arg His Val Glu Leu 
            20                  25                  30          


Ala Arg Val Gly Gln Leu Val Glu Val Asp Thr Leu Glu His Val Gln 
        35                  40                  45              


His Ile Ile Gly Gly Ala Gly Asn Asp Ser Ile Thr Gly Asn Ala His 
    50                  55                  60                  


Asp Asn Phe Leu Ala Gly Gly Ser Gly Asp Asp Arg Leu Asp Gly Gly 
65                  70                  75                  80  


Ala Gly Asn Asp Thr Leu Val Gly Gly Glu Gly Gln Asn Thr Val Ile 
                85                  90                  95      


Gly Gly Ala Gly Asp Asp Val Phe Leu Gln Asp Leu Gly Val Trp Ser 
            100                 105                 110         


Asn Gln Leu Asp Gly Gly Ala Gly Val Asp Thr Val Lys Tyr Asn Val 
        115                 120                 125             


His Gln Pro Ser Glu Glu Arg Leu Glu Arg Met Gly Asp Thr Gly Ile 
    130                 135                 140                 


His Ala Asp Leu Gln Lys Gly Thr Val Glu Lys Trp Pro Ala Leu Asn 
145                 150                 155                 160 


Leu Phe Ser Val Asp His Val Lys Asn Ile Glu Asn Leu His Gly Ser 
                165                 170                 175     


Arg Leu Asn Asp Arg Ile Ala Gly Asp Asp Gln Asp Asn Glu Leu Trp 
            180                 185                 190         


Gly His Asp Gly Asn Asp Thr Ile Arg Gly Arg Gly Gly Asp Asp Ile 
        195                 200                 205             


Leu Arg Gly Gly Leu Gly Leu Asp Thr Leu Tyr Gly Glu Asp Gly Asn 
    210                 215                 220                 


Asp Ile Phe Leu Gln Asp Asp Glu Thr Val Ser Asp Asp Ile Asp Gly 
225                 230                 235                 240 


Gly Ala Gly Leu Asp Thr Val Asp Tyr Ser Ala Met Ile His Pro Gly 
                245                 250                 255     


Arg Ile Val Ala Pro His Glu Tyr Gly Phe Gly Ile Glu Ala Asp Leu 
            260                 265                 270         


Ser Arg Glu Trp Val Arg Lys Ala Ser Ala Leu Gly Val Asp Tyr Tyr 
        275                 280                 285             


Asp Asn Val Arg Asn Val Glu Asn Val Ile Gly Thr Ser Met Lys Asp 
    290                 295                 300                 


Val Leu Ile Gly Asp Ala Gln Ala Asn Thr Leu Met Gly Gln Gly Gly 
305                 310                 315                 320 


Asp Asp Thr Val Arg Gly Gly Asp Gly Asp Asp Leu Leu Phe Gly Gly 
                325                 330                 335     


Asp Gly Asn Asp Met Leu Tyr Gly Asp Ala Gly Asn Asp Thr Leu Tyr 
            340                 345                 350         


Gly Gly Leu Gly Asp Asp Thr Leu Glu Gly Gly Ala Gly Asn Asp Trp 
        355                 360                 365             


Phe Gly Gln Thr Gln Ala Arg Glu His Asp Val Leu Arg Gly Gly Asp 
    370                 375                 380                 


Gly Val Asp Thr Val Asp Tyr Ser Gln Thr Gly Ala His Ala Gly Ile 
385                 390                 395                 400 


Ala Ala Gly Arg Ile Gly Leu Gly Ile Leu Ala Asp Leu Gly Ala Gly 
                405                 410                 415     


Arg Val Asp Lys Leu Gly Glu Ala Gly Ser Ser Ala Tyr Asp Thr Val 
            420                 425                 430         


Ser Gly Ile Glu Asn Val Val Gly Thr Glu Leu Ala Asp Arg Ile Thr 
        435                 440                 445             


Gly Asp Ala Gln Ala Asn Val Leu Arg Gly Ala Gly Gly Ala Asp Val 
    450                 455                 460                 


Leu Ala Gly Gly Glu Gly Asp Asp Val Leu Leu Gly Gly Asp Gly Asp 
465                 470                 475                 480 


Asp Gln Leu Ser Gly Asp Ala Gly Arg Asp Arg Leu Tyr Gly Glu Ala 
                485                 490                 495     


Gly Asp Asp Trp Phe Phe Gln Asp Ala Ala Asn Ala Gly Asn Leu Leu 
            500                 505                 510         


Asp Gly Gly Asp Gly Arg Asp Thr Val Asp Phe Ser Gly Pro Gly Arg 
        515                 520                 525             


Gly Leu Asp Ala Gly Ala Lys Gly Val Phe Leu Ser Leu Gly Lys Gly 
    530                 535                 540                 


Phe Ala Ser Leu Met Asp Glu Pro Glu Thr Ser Asn Val Leu Arg Asn 
545                 550                 555                 560 


Ile Glu Asn Ala Val Gly Ser Ala Arg Asp Asp Val Leu Ile Gly Asp 
                565                 570                 575     


Ala Gly Ala Asn Val Leu Asn Gly Leu Ala Gly Asn Asp Val Leu Ser 
            580                 585                 590         


Gly Gly Ala Gly Asp Asp Val Leu Leu Gly Asp Glu Gly Ser Asp Leu 
        595                 600                 605             


Leu Ser Gly Asp Ala Gly Asn Asp Asp Leu Phe Gly Gly Gln Gly Asp 
    610                 615                 620                 


Asp Thr Tyr Leu Phe Gly Val Gly Tyr Gly His Asp Thr Ile Tyr Glu 
625                 630                 635                 640 


Ser Gly Gly Gly His Asp Thr Ile Arg Ile Asn Ala Gly Ala Asp Gln 
                645                 650                 655     


Leu Trp Phe Ala Arg Gln Gly Asn Asp Leu Glu Ile Arg Ile Leu Gly 
            660                 665                 670         


Thr Asp Asp Ala Leu Thr Val His Asp Trp Tyr Arg Asp Ala Asp His 
        675                 680                 685             


Arg Val Glu Ile Ile His Ala Ala Asn Gln Ala Val Asp Gln Ala Gly 
    690                 695                 700                 


Ile Glu Lys Leu Val Glu Ala Met Ala Gln Tyr Pro Asp Pro Gly Gly 
705                 710                 715                 720 


His His His His His His 
                725     


<210>  9
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker 1

<400>  9

Gly Ser Gly Ser 
1               


<210>  10
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 2

<400>  10

Gly Ser Gly Gly Gly Gly 
1               5       


<210>  11
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker 3

<400>  11

Gly Ser Gly Ser Gly Gly Gly Gly 
1               5               


<210>  12
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His tag 1

<400>  12

Gly Gly His His His His His His 
1               5               


<210>  13
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His tag 2

<400>  13

Gly His His His His His His 
1               5           


<210>  14
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His tag 3

<400>  14

His His His His His His 
1               5       


<210>  15
<211>  399
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AC domain (from residue 1 to residue 400).

<400>  15

Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu Ser 
1               5                   10                  15      


Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys Glu 
            20                  25                  30          


Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr Ser 
        35                  40                  45              


Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala Lys 
    50                  55                  60                  


Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro Asn 
65                  70                  75                  80  


Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala Asp 
                85                  90                  95      


Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp Leu 
            100                 105                 110         


Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu Val 
        115                 120                 125             


Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr Glu 
    130                 135                 140                 


Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala Val 
145                 150                 155                 160 


Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val Ile 
                165                 170                 175     


Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Ile Asp Met Phe Ala 
            180                 185                 190         


Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg Ser Ser Val 
        195                 200                 205             


Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr Arg Arg Ala 
    210                 215                 220                 


Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile Asp Leu Leu 
225                 230                 235                 240 


Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr Glu Ala 
                245                 250                 255     


Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val Ile Thr 
            260                 265                 270         


Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala His Ala 
        275                 280                 285             


Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln Asn Asn Pro 
    290                 295                 300                 


Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr Gly Glu 
305                 310                 315                 320 


Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile Gly Gln Gln 
                325                 330                 335     


Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr Gly Val Ala 
            340                 345                 350         


Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro Gly Val Pro 
        355                 360                 365             


Ser Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr Val Pro Ala 
    370                 375                 380                 


Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu Arg Gln 
385                 390                 395                 


<210>  16
<211>  401
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AC domain (from residue 1 to residue 400) with GS insertion

<400>  16

Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu Ser 
1               5                   10                  15      


Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys Glu 
            20                  25                  30          


Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr Ser 
        35                  40                  45              


Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala Lys 
    50                  55                  60                  


Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro Asn 
65                  70                  75                  80  


Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala Asp 
                85                  90                  95      


Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp Leu 
            100                 105                 110         


Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu Val 
        115                 120                 125             


Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr Glu 
    130                 135                 140                 


Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala Val 
145                 150                 155                 160 


Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val Ile 
                165                 170                 175     


Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Gly Ser Ile Asp Met 
            180                 185                 190         


Phe Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg Ser 
        195                 200                 205             


Ser Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr Arg 
    210                 215                 220                 


Arg Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile Asp 
225                 230                 235                 240 


Leu Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr 
                245                 250                 255     


Glu Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val 
            260                 265                 270         


Ile Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala 
        275                 280                 285             


His Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln Asn 
    290                 295                 300                 


Asn Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr 
305                 310                 315                 320 


Gly Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile Gly 
                325                 330                 335     


Gln Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr Gly 
            340                 345                 350         


Val Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro Gly 
        355                 360                 365             


Val Pro Ser Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr Val 
    370                 375                 380                 


Pro Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu Arg 
385                 390                 395                 400 


Gln 
    


<210>  17
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AC domain (from residue 360 to residue 493).

<400>  17

Asp Gly Leu Gly Ala Ala Pro Gly Val Pro Ser Gly Arg Ser Lys Phe 
1               5                   10                  15      


Ser Pro Asp Val Leu Glu Thr Val Pro Ala Ser Pro Gly Leu Arg Arg 
            20                  25                  30          


Pro Ser Leu Gly Ala Val Glu Arg Gln Asp Ser Gly Tyr Asp Ser Leu 
        35                  40                  45              


Asp Gly Val Gly Ser Arg Ser Phe Ser Leu Gly Glu Val Ser Asp Met 
    50                  55                  60                  


Ala Ala Val Glu Ala Ala Glu Leu Glu Met Thr Arg Gln Val Leu His 
65                  70                  75                  80  


Ala Gly Ala Arg Gln Asp Asp Ala Glu Pro Gly Val Ser Gly Ala Ser 
                85                  90                  95      


Ala His Trp Gly Gln Arg Ala Leu Gln Gly Ala Gln Ala Val Ala Ala 
            100                 105                 110         


Ala Gln Arg Leu Val His Ala Ile Ala Leu Met Thr Gln Phe Gly Arg 
        115                 120                 125             


Ala Gly Ser Thr Asn Thr 
    130                 


<210>  18
<211>  400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AC domain (from residue 1 to residue 400).

<400>  18

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Ile Asp Met Phe 
            180                 185                 190         


Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg Ser Ser 
        195                 200                 205             


Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr Arg Arg 
    210                 215                 220                 


Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile Asp Leu 
225                 230                 235                 240 


Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr Glu 
                245                 250                 255     


Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val Ile 
            260                 265                 270         


Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala His 
        275                 280                 285             


Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln Asn Asn 
    290                 295                 300                 


Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr Gly 
305                 310                 315                 320 


Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile Gly Gln 
                325                 330                 335     


Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr Gly Val 
            340                 345                 350         


Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro Gly Val 
        355                 360                 365             


Pro Ser Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr Val Pro 
    370                 375                 380                 


Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu Arg Gln 
385                 390                 395                 400 


<210>  19
<211>  402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AC domain (from residue 1 to residue 400) with GS insertion

<400>  19

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Gly Ser Ile Asp 
            180                 185                 190         


Met Phe Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg 
        195                 200                 205             


Ser Ser Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr 
    210                 215                 220                 


Arg Arg Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile 
225                 230                 235                 240 


Asp Leu Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly 
                245                 250                 255     


Thr Glu Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly 
            260                 265                 270         


Val Ile Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg 
        275                 280                 285             


Ala His Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln 
    290                 295                 300                 


Asn Asn Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala 
305                 310                 315                 320 


Thr Gly Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile 
                325                 330                 335     


Gly Gln Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr 
            340                 345                 350         


Gly Val Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro 
        355                 360                 365             


Gly Val Pro Ser Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr 
    370                 375                 380                 


Val Pro Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu 
385                 390                 395                 400 


Arg Gln 
        


<210>  20
<211>  135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AC domain (from residue 360 to residue 493).

<400>  20

Met Asp Gly Leu Gly Ala Ala Pro Gly Val Pro Ser Gly Arg Ser Lys 
1               5                   10                  15      


Phe Ser Pro Asp Val Leu Glu Thr Val Pro Ala Ser Pro Gly Leu Arg 
            20                  25                  30          


Arg Pro Ser Leu Gly Ala Val Glu Arg Gln Asp Ser Gly Tyr Asp Ser 
        35                  40                  45              


Leu Asp Gly Val Gly Ser Arg Ser Phe Ser Leu Gly Glu Val Ser Asp 
    50                  55                  60                  


Met Ala Ala Val Glu Ala Ala Glu Leu Glu Met Thr Arg Gln Val Leu 
65                  70                  75                  80  


His Ala Gly Ala Arg Gln Asp Asp Ala Glu Pro Gly Val Ser Gly Ala 
                85                  90                  95      


Ser Ala His Trp Gly Gln Arg Ala Leu Gln Gly Ala Gln Ala Val Ala 
            100                 105                 110         


Ala Ala Gln Arg Leu Val His Ala Ile Ala Leu Met Thr Gln Phe Gly 
        115                 120                 125             


Arg Ala Gly Ser Thr Asn Thr 
    130                 135 


<210>  21
<211>  410
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AC domain (from residue 1 to residue 400). Cytoplasmic expression
       of his-tagged (composed of GGHHHHHH sequence) domain

<400>  21

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Gly Ser Ile Asp 
            180                 185                 190         


Met Phe Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg 
        195                 200                 205             


Ser Ser Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr 
    210                 215                 220                 


Arg Arg Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile 
225                 230                 235                 240 


Asp Leu Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly 
                245                 250                 255     


Thr Glu Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly 
            260                 265                 270         


Val Ile Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg 
        275                 280                 285             


Ala His Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln 
    290                 295                 300                 


Asn Asn Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala 
305                 310                 315                 320 


Thr Gly Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile 
                325                 330                 335     


Gly Gln Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr 
            340                 345                 350         


Gly Val Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro 
        355                 360                 365             


Gly Val Pro Ser Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr 
    370                 375                 380                 


Val Pro Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu 
385                 390                 395                 400 


Arg Gln Gly Gly His His His His His His 
                405                 410 


<210>  22
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AC domain (from residue 360 to residue 493). Cytoplasmic 
       expression of his-tagged (composed of GGHHHHHH sequence) domain.

<400>  22

Met Asp Gly Leu Gly Ala Ala Pro Gly Val Pro Ser Gly Arg Ser Lys 
1               5                   10                  15      


Phe Ser Pro Asp Val Leu Glu Thr Val Pro Ala Ser Pro Gly Leu Arg 
            20                  25                  30          


Arg Pro Ser Leu Gly Ala Val Glu Arg Gln Asp Ser Gly Tyr Asp Ser 
        35                  40                  45              


Leu Asp Gly Val Gly Ser Arg Ser Phe Ser Leu Gly Glu Val Ser Asp 
    50                  55                  60                  


Met Ala Ala Val Glu Ala Ala Glu Leu Glu Met Thr Arg Gln Val Leu 
65                  70                  75                  80  


His Ala Gly Ala Arg Gln Asp Asp Ala Glu Pro Gly Val Ser Gly Ala 
                85                  90                  95      


Ser Ala His Trp Gly Gln Arg Ala Leu Gln Gly Ala Gln Ala Val Ala 
            100                 105                 110         


Ala Ala Gln Arg Leu Val His Ala Ile Ala Leu Met Thr Gln Phe Gly 
        115                 120                 125             


Arg Ala Gly Ser Thr Asn Thr Gly Gly His His His His His His 
    130                 135                 140             


<210>  23
<211>  698
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adenylate cyclase fragment without His tag

<400>  23

Met Thr Glu Asn Val Gln Tyr Arg His Val Glu Leu Ala Arg Val Gly 
1               5                   10                  15      


Gln Leu Val Glu Val Asp Thr Leu Glu His Val Gln His Ile Ile Gly 
            20                  25                  30          


Gly Ala Gly Asn Asp Ser Ile Thr Gly Asn Ala His Asp Asn Phe Leu 
        35                  40                  45              


Ala Gly Gly Ser Gly Asp Asp Arg Leu Asp Gly Gly Ala Gly Asn Asp 
    50                  55                  60                  


Thr Leu Val Gly Gly Glu Gly Gln Asn Thr Val Ile Gly Gly Ala Gly 
65                  70                  75                  80  


Asp Asp Val Phe Leu Gln Asp Leu Gly Val Trp Ser Asn Gln Leu Asp 
                85                  90                  95      


Gly Gly Ala Gly Val Asp Thr Val Lys Tyr Asn Val His Gln Pro Ser 
            100                 105                 110         


Glu Glu Arg Leu Glu Arg Met Gly Asp Thr Gly Ile His Ala Asp Leu 
        115                 120                 125             


Gln Lys Gly Thr Val Glu Lys Trp Pro Ala Leu Asn Leu Phe Ser Val 
    130                 135                 140                 


Asp His Val Lys Asn Ile Glu Asn Leu His Gly Ser Arg Leu Asn Asp 
145                 150                 155                 160 


Arg Ile Ala Gly Asp Asp Gln Asp Asn Glu Leu Trp Gly His Asp Gly 
                165                 170                 175     


Asn Asp Thr Ile Arg Gly Arg Gly Gly Asp Asp Ile Leu Arg Gly Gly 
            180                 185                 190         


Leu Gly Leu Asp Thr Leu Tyr Gly Glu Asp Gly Asn Asp Ile Phe Leu 
        195                 200                 205             


Gln Asp Asp Glu Thr Val Ser Asp Asp Ile Asp Gly Gly Ala Gly Leu 
    210                 215                 220                 


Asp Thr Val Asp Tyr Ser Ala Met Ile His Pro Gly Arg Ile Val Ala 
225                 230                 235                 240 


Pro His Glu Tyr Gly Phe Gly Ile Glu Ala Asp Leu Ser Arg Glu Trp 
                245                 250                 255     


Val Arg Lys Ala Ser Ala Leu Gly Val Asp Tyr Tyr Asp Asn Val Arg 
            260                 265                 270         


Asn Val Glu Asn Val Ile Gly Thr Ser Met Lys Asp Val Leu Ile Gly 
        275                 280                 285             


Asp Ala Gln Ala Asn Thr Leu Met Gly Gln Gly Gly Asp Asp Thr Val 
    290                 295                 300                 


Arg Gly Gly Asp Gly Asp Asp Leu Leu Phe Gly Gly Asp Gly Asn Asp 
305                 310                 315                 320 


Met Leu Tyr Gly Asp Ala Gly Asn Asp Thr Leu Tyr Gly Gly Leu Gly 
                325                 330                 335     


Asp Asp Thr Leu Glu Gly Gly Ala Gly Asn Asp Trp Phe Gly Gln Thr 
            340                 345                 350         


Gln Ala Arg Glu His Asp Val Leu Arg Gly Gly Asp Gly Val Asp Thr 
        355                 360                 365             


Val Asp Tyr Ser Gln Thr Gly Ala His Ala Gly Ile Ala Ala Gly Arg 
    370                 375                 380                 


Ile Gly Leu Gly Ile Leu Ala Asp Leu Gly Ala Gly Arg Val Asp Lys 
385                 390                 395                 400 


Leu Gly Glu Ala Gly Ser Ser Ala Tyr Asp Thr Val Ser Gly Ile Glu 
                405                 410                 415     


Asn Val Val Gly Thr Glu Leu Ala Asp Arg Ile Thr Gly Asp Ala Gln 
            420                 425                 430         


Ala Asn Val Leu Arg Gly Ala Gly Gly Ala Asp Val Leu Ala Gly Gly 
        435                 440                 445             


Glu Gly Asp Asp Val Leu Leu Gly Gly Asp Gly Asp Asp Gln Leu Ser 
    450                 455                 460                 


Gly Asp Ala Gly Arg Asp Arg Leu Tyr Gly Glu Ala Gly Asp Asp Trp 
465                 470                 475                 480 


Phe Phe Gln Asp Ala Ala Asn Ala Gly Asn Leu Leu Asp Gly Gly Asp 
                485                 490                 495     


Gly Arg Asp Thr Val Asp Phe Ser Gly Pro Gly Arg Gly Leu Asp Ala 
            500                 505                 510         


Gly Ala Lys Gly Val Phe Leu Ser Leu Gly Lys Gly Phe Ala Ser Leu 
        515                 520                 525             


Met Asp Glu Pro Glu Thr Ser Asn Val Leu Arg Asn Ile Glu Asn Ala 
    530                 535                 540                 


Val Gly Ser Ala Arg Asp Asp Val Leu Ile Gly Asp Ala Gly Ala Asn 
545                 550                 555                 560 


Val Leu Asn Gly Leu Ala Gly Asn Asp Val Leu Ser Gly Gly Ala Gly 
                565                 570                 575     


Asp Asp Val Leu Leu Gly Asp Glu Gly Ser Asp Leu Leu Ser Gly Asp 
            580                 585                 590         


Ala Gly Asn Asp Asp Leu Phe Gly Gly Gln Gly Asp Asp Thr Tyr Leu 
        595                 600                 605             


Phe Gly Val Gly Tyr Gly His Asp Thr Ile Tyr Glu Ser Gly Gly Gly 
    610                 615                 620                 


His Asp Thr Ile Arg Ile Asn Ala Gly Ala Asp Gln Leu Trp Phe Ala 
625                 630                 635                 640 


Arg Gln Gly Asn Asp Leu Glu Ile Arg Ile Leu Gly Thr Asp Asp Ala 
                645                 650                 655     


Leu Thr Val His Asp Trp Tyr Arg Asp Ala Asp His Arg Val Glu Ile 
            660                 665                 670         


Ile His Ala Ala Asn Gln Ala Val Asp Gln Ala Gly Ile Glu Lys Leu 
        675                 680                 685             


Val Glu Ala Met Ala Gln Tyr Pro Asp Pro 
    690                 695             


