               SEQUENCE LISTING

<110> ENZA ZADEN BEHEER B.V.

<120> RESISTANCE GENE AND LETTUCE PLANT RESISTANT TO DOWNY MILDEW

<130> 4/2XO43/63

<160> 12

<170> BiSSAP 1.3.6

<210> 1
<211> 1758
<212> DNA
<213> Lactuca sativa


<400> 1
atggcgctta agcttccagc tgctgaagct gcaaatgttg cgattcagtc aattggatgt      60

ggttatgata tctcgttaga tctgaggctt aattatcgca tgcgtcgtta tcattcagat     120

attggttcta atccatatag aaattgccgt ttgatagaaa ttgaggaaga tgaaggtaga     180

gatattgtgc ttcctggtgg gcttctactt ctcaatgttc ccgaatccat caaatgtcat     240

gaaagcgagc atacacgact tcattctgat gttctttctt ttccgcagat gtcggagcaa     300

ttcaatcagg aattatcatt atccggcaaa attccttcag gcctcttcaa ttatgtgttt     360

gatttttctg gaaattggaa gaaagatgca tcaagtacta aaaccctagc ttttgatggt     420

gttttcatct ctttgtacac ggttgcatta gaagaatcac acatggttct ttgtgatcac     480

gtcaagaaag ctgttccatc ttcatgggag cctgctttgt tagcaaggtt tatcgaaaaa     540

tatggcactc atgtaattgt gggtgtaaaa atgggtggaa aagatgtgat atatatgaaa     600

caacagcata catcatccct tgagcctgct gatgtacaga aaaagttaaa ggaaatggca     660

gacaagagat ttttggactc taaacagatt tcacaaaatg acaagtatga catcacacat     720

ctaagatctg cagacttaga cacctctagc tcttattctt acaaggagca tcttttaagc     780

atttgcagaa ggagaggtgg gagtgatgac aggaacctaa aacataacga gtggttacaa     840

accgtacaat cagaaccaga tgtaatcaca atgtcctttg ttccaataag cttgttgcta     900

aagggtgtta tgggaagtgg attcttaagt catgccataa atctttattt acgctataaa     960

cctccatttg aagaccttcc tcagtttctg gacttccagc taccgacaca gtgggcccct    1020

gtattcggtt tgttgtctcg tggtccacaa catgagcagc gaagtgtgac atctttacag    1080

ttcagctttt ttgggaccaa gctctacgtg aacacctatc ctgtcgatgt aggtaagagg    1140

cctgtaacag gcctccgact ttccctcaat tgtgaaagaa acaatcagtt agagatccat    1200

ctccagcacc tctccgccgt cccaaaattc gtccaactag aagactccgt aaccgaaatc    1260

ctcaatttcg attcccacga tcataaagac tacgaaaagg tccaagcgaa aaatttcgac    1320

catgtttgca cagccactgt cgagtctgaa gacgaattcg caatcgtaac cggggcccag    1380

ttacatgtcg gggatcacgg gtctaaaaaa gtcctcttcc tacgcctcca cttctcaaaa    1440

ctacatggtt gcatcgcaaa taggaatccg gaatgggaag tgtctccggt tcagaaactg    1500

gattcttcaa tttctttcat ggaggtggtc tacgcacgac aatttcaagt cccgcatgcc    1560

ccacttcacg aaccgaaatt tgtgggcccg acggagacaa tgcagaaact attgaagttt    1620

gtggacacaa cggagatgaa gcggggcccg caagatagcc cagggtattg ggttgtcata    1680

ggggctagac ttgttgtgga taagggaaag atttctcttc gagtcaagta cgctttgcct    1740

cagatgttaa cccgatga                                                  1758


<210> 2
<211> 585
<212> PRT
<213> Lactuca sativa


<400> 2
Met Ala Leu Lys Leu Pro Ala Ala Glu Ala Ala Asn Val Ala Ile Gln 
1               5                   10                  15      
Ser Ile Gly Cys Gly Tyr Asp Ile Ser Leu Asp Leu Arg Leu Asn Tyr 
            20                  25                  30          
Arg Met Arg Arg Tyr His Ser Asp Ile Gly Ser Asn Pro Tyr Arg Asn 
        35                  40                  45              
Cys Arg Leu Ile Glu Ile Glu Glu Asp Glu Gly Arg Asp Ile Val Leu 
    50                  55                  60                  
Pro Gly Gly Leu Leu Leu Leu Asn Val Pro Glu Ser Ile Lys Cys His 
65                  70                  75                  80  
Glu Ser Glu His Thr Arg Leu His Ser Asp Val Leu Ser Phe Pro Gln 
                85                  90                  95      
Met Ser Glu Gln Phe Asn Gln Glu Leu Ser Leu Ser Gly Lys Ile Pro 
            100                 105                 110         
Ser Gly Leu Phe Asn Tyr Val Phe Asp Phe Ser Gly Asn Trp Lys Lys 
        115                 120                 125             
Asp Ala Ser Ser Thr Lys Thr Leu Ala Phe Asp Gly Val Phe Ile Ser 
    130                 135                 140                 
Leu Tyr Thr Val Ala Leu Glu Glu Ser His Met Val Leu Cys Asp His 
145                 150                 155                 160 
Val Lys Lys Ala Val Pro Ser Ser Trp Glu Pro Ala Leu Leu Ala Arg 
                165                 170                 175     
Phe Ile Glu Lys Tyr Gly Thr His Val Ile Val Gly Val Lys Met Gly 
            180                 185                 190         
Gly Lys Asp Val Ile Tyr Met Lys Gln Gln His Thr Ser Ser Leu Glu 
        195                 200                 205             
Pro Ala Asp Val Gln Lys Lys Leu Lys Glu Met Ala Asp Lys Arg Phe 
    210                 215                 220                 
Leu Asp Ser Lys Gln Ile Ser Gln Asn Asp Lys Tyr Asp Ile Thr His 
225                 230                 235                 240 
Leu Arg Ser Ala Asp Leu Asp Thr Ser Ser Ser Tyr Ser Tyr Lys Glu 
                245                 250                 255     
His Leu Leu Ser Ile Cys Arg Arg Arg Gly Gly Ser Asp Asp Arg Asn 
            260                 265                 270         
Leu Lys His Asn Glu Trp Leu Gln Thr Val Gln Ser Glu Pro Asp Val 
        275                 280                 285             
Ile Thr Met Ser Phe Val Pro Ile Ser Leu Leu Leu Lys Gly Val Met 
    290                 295                 300                 
Gly Ser Gly Phe Leu Ser His Ala Ile Asn Leu Tyr Leu Arg Tyr Lys 
305                 310                 315                 320 
Pro Pro Phe Glu Asp Leu Pro Gln Phe Leu Asp Phe Gln Leu Pro Thr 
                325                 330                 335     
Gln Trp Ala Pro Val Phe Gly Leu Leu Ser Arg Gly Pro Gln His Glu 
            340                 345                 350         
Gln Arg Ser Val Thr Ser Leu Gln Phe Ser Phe Phe Gly Thr Lys Leu 
        355                 360                 365             
Tyr Val Asn Thr Tyr Pro Val Asp Val Gly Lys Arg Pro Val Thr Gly 
    370                 375                 380                 
Leu Arg Leu Ser Leu Asn Cys Glu Arg Asn Asn Gln Leu Glu Ile His 
385                 390                 395                 400 
Leu Gln His Leu Ser Ala Val Pro Lys Phe Val Gln Leu Glu Asp Ser 
                405                 410                 415     
Val Thr Glu Ile Leu Asn Phe Asp Ser His Asp His Lys Asp Tyr Glu 
            420                 425                 430         
Lys Val Gln Ala Lys Asn Phe Asp His Val Cys Thr Ala Thr Val Glu 
        435                 440                 445             
Ser Glu Asp Glu Phe Ala Ile Val Thr Gly Ala Gln Leu His Val Gly 
    450                 455                 460                 
Asp His Gly Ser Lys Lys Val Leu Phe Leu Arg Leu His Phe Ser Lys 
465                 470                 475                 480 
Leu His Gly Cys Ile Ala Asn Arg Asn Pro Glu Trp Glu Val Ser Pro 
                485                 490                 495     
Val Gln Lys Leu Asp Ser Ser Ile Ser Phe Met Glu Val Val Tyr Ala 
            500                 505                 510         
Arg Gln Phe Gln Val Pro His Ala Pro Leu His Glu Pro Lys Phe Val 
        515                 520                 525             
Gly Pro Thr Glu Thr Met Gln Lys Leu Leu Lys Phe Val Asp Thr Thr 
    530                 535                 540                 
Glu Met Lys Arg Gly Pro Gln Asp Ser Pro Gly Tyr Trp Val Val Ile 
545                 550                 555                 560 
Gly Ala Arg Leu Val Val Asp Lys Gly Lys Ile Ser Leu Arg Val Lys 
                565                 570                 575     
Tyr Ala Leu Pro Gln Met Leu Thr Arg 
            580                 585 

<210> 3
<211> 1775
<212> DNA
<213> Lactuca serriola


<400> 3
atggcgctta agcttccagc tgctgaagct gcaaatgttg cgattcagtc aattggatgt      60

ggttatgata tcgcgttaga tctgaggctt aattatcgca tgcgtcgtta tcattcagat     120

attggttcta atccatatag aaattgccgt ttgatagaaa ttgaggaaga tgaaggtaga     180

gatattgtgc ttcctggtgg gcttctactt ctcaatgttc ccgaatccat caaatgtcat     240

gaaagcgagt atacacgact tcattctgat gttctttctt ttccgcagat gtcggagcaa     300

ttcaatcagg aattatcatt atccggcaaa attccttcag gcctcttcaa ttatgtgttt     360

gatttttctg gaaattggaa gaaagatgca tcaagtacta aaaccctagc ttttgatggt     420

gttttcatct ctttgtacac ggttgcatta gaagaatcac acatggttct ttgtgatcac     480

gtcaagaaag ctgttccatc ttcatgggag cctgctttgt tagcaaggtt tatggaaaaa     540

tttggcactc atgtaattgt gggtgtaaaa atgggtggaa aagatgtgat atatatgaaa     600

caacagcatg catcatccct tgaacctgct gatgtacaga aaaagttaaa ggaaatggca     660

gacaagagat ttttggactc taaacagatt tcacaaaatg acacgtttga catcacacat     720

ctaagatctg cagacttaga cacctctagc tcttattctt acacaaaaaa tgacaaggag     780

catcttttaa gcatttgcag aaggagaggt gggagtgatg acaggaacct aaaacataac     840

gagtggttac aaaccgtaca atcagaacca gatgtaatca caatgtcctt tgttccaata     900

agcttgttgc taaagggtgt tatgggaagt ggattcttaa gtcatgccat aaatctttat     960

ttacgctata aacctccatt tgaagagctt cctcagtttc tggacttcca gctaccgaca    1020

cagtgggccc ctgtattcgg tttgttgtct cgtggtccac aacatgagca gcgaagtgtg    1080

acatctttac agttcagctt ttttgggacc aagctctacg tgaacaccta tcctgtcgat    1140

gtaggtaaga ggcctgtaac aggcctccga ctttccctca attgtgaaag aaacaatcag    1200

ttagagatcc atctccagca cctctccgcc gtcccaaaat tcgtccaact agaagactcc    1260

gtaaccgaaa tcctcaattt cgattcccac gatcataaag actacgaaaa ggtccaagcg    1320

aaaaatttcg accatgtttg cacagcctct gtcgagtctg aagacgaatt cgcaatcgta    1380

accggggccc agttacatgt cggggatcac gggtctaaaa aagtcctctt cctacgcctc    1440

cacttctcaa aactacatgg ttgcatcgca aataggaatc cggaatggga agtgtctccg    1500

gttcagaaac tggattcttc aatttctttc atggaggtgg tctacgcacg acaatttcaa    1560

gtcccgcatg ccccacttca cgaaccgaaa tttgtgggcc cgacggagac gatgcagaag    1620

ctattgaagt ttgtggacac aacggagatg aagcggggcc cgcaagatag cccagggtat    1680

tgggttgtaa taggggctag acttgttgtg gataagggaa agatttctct tcgagtcaag    1740

tacgctttgc ctcagatatt aatcgatgat tagaa                               1775


<210> 4
<211> 590
<212> PRT
<213> Lactuca serriola


<400> 4
Met Ala Leu Lys Leu Pro Ala Ala Glu Ala Ala Asn Val Ala Ile Gln 
1               5                   10                  15      
Ser Ile Gly Cys Gly Tyr Asp Ile Ala Leu Asp Leu Arg Leu Asn Tyr 
            20                  25                  30          
Arg Met Arg Arg Tyr His Ser Asp Ile Gly Ser Asn Pro Tyr Arg Asn 
        35                  40                  45              
Cys Arg Leu Ile Glu Ile Glu Glu Asp Glu Gly Arg Asp Ile Val Leu 
    50                  55                  60                  
Pro Gly Gly Leu Leu Leu Leu Asn Val Pro Glu Ser Ile Lys Cys His 
65                  70                  75                  80  
Glu Ser Glu Tyr Thr Arg Leu His Ser Asp Val Leu Ser Phe Pro Gln 
                85                  90                  95      
Met Ser Glu Gln Phe Asn Gln Glu Leu Ser Leu Ser Gly Lys Ile Pro 
            100                 105                 110         
Ser Gly Leu Phe Asn Tyr Val Phe Asp Phe Ser Gly Asn Trp Lys Lys 
        115                 120                 125             
Asp Ala Ser Ser Thr Lys Thr Leu Ala Phe Asp Gly Val Phe Ile Ser 
    130                 135                 140                 
Leu Tyr Thr Val Ala Leu Glu Glu Ser His Met Val Leu Cys Asp His 
145                 150                 155                 160 
Val Lys Lys Ala Val Pro Ser Ser Trp Glu Pro Ala Leu Leu Ala Arg 
                165                 170                 175     
Phe Met Glu Lys Phe Gly Thr His Val Ile Val Gly Val Lys Met Gly 
            180                 185                 190         
Gly Lys Asp Val Ile Tyr Met Lys Gln Gln His Ala Ser Ser Leu Glu 
        195                 200                 205             
Pro Ala Asp Val Gln Lys Lys Leu Lys Glu Met Ala Asp Lys Arg Phe 
    210                 215                 220                 
Leu Asp Ser Lys Gln Ile Ser Gln Asn Asp Thr Phe Asp Ile Thr His 
225                 230                 235                 240 
Leu Arg Ser Ala Asp Leu Asp Thr Ser Ser Ser Tyr Ser Tyr Thr Lys 
                245                 250                 255     
Asn Asp Lys Glu His Leu Leu Ser Ile Cys Arg Arg Arg Gly Gly Ser 
            260                 265                 270         
Asp Asp Arg Asn Leu Lys His Asn Glu Trp Leu Gln Thr Val Gln Ser 
        275                 280                 285             
Glu Pro Asp Val Ile Thr Met Ser Phe Val Pro Ile Ser Leu Leu Leu 
    290                 295                 300                 
Lys Gly Val Met Gly Ser Gly Phe Leu Ser His Ala Ile Asn Leu Tyr 
305                 310                 315                 320 
Leu Arg Tyr Lys Pro Pro Phe Glu Glu Leu Pro Gln Phe Leu Asp Phe 
                325                 330                 335     
Gln Leu Pro Thr Gln Trp Ala Pro Val Phe Gly Leu Leu Ser Arg Gly 
            340                 345                 350         
Pro Gln His Glu Gln Arg Ser Val Thr Ser Leu Gln Phe Ser Phe Phe 
        355                 360                 365             
Gly Thr Lys Leu Tyr Val Asn Thr Tyr Pro Val Asp Val Gly Lys Arg 
    370                 375                 380                 
Pro Val Thr Gly Leu Arg Leu Ser Leu Asn Cys Glu Arg Asn Asn Gln 
385                 390                 395                 400 
Leu Glu Ile His Leu Gln His Leu Ser Ala Val Pro Lys Phe Val Gln 
                405                 410                 415     
Leu Glu Asp Ser Val Thr Glu Ile Leu Asn Phe Asp Ser His Asp His 
            420                 425                 430         
Lys Asp Tyr Glu Lys Val Gln Ala Lys Asn Phe Asp His Val Cys Thr 
        435                 440                 445             
Ala Ser Val Glu Ser Glu Asp Glu Phe Ala Ile Val Thr Gly Ala Gln 
    450                 455                 460                 
Leu His Val Gly Asp His Gly Ser Lys Lys Val Leu Phe Leu Arg Leu 
465                 470                 475                 480 
His Phe Ser Lys Leu His Gly Cys Ile Ala Asn Arg Asn Pro Glu Trp 
                485                 490                 495     
Glu Val Ser Pro Val Gln Lys Leu Asp Ser Ser Ile Ser Phe Met Glu 
            500                 505                 510         
Val Val Tyr Ala Arg Gln Phe Gln Val Pro His Ala Pro Leu His Glu 
        515                 520                 525             
Pro Lys Phe Val Gly Pro Thr Glu Thr Met Gln Lys Leu Leu Lys Phe 
    530                 535                 540                 
Val Asp Thr Thr Glu Met Lys Arg Gly Pro Gln Asp Ser Pro Gly Tyr 
545                 550                 555                 560 
Trp Val Val Ile Gly Ala Arg Leu Val Val Asp Lys Gly Lys Ile Ser 
                565                 570                 575     
Leu Arg Val Lys Tyr Ala Leu Pro Gln Ile Leu Ile Asp Asp 
            580                 585                 590 

<210> 5
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> MACPF1R_F

<400> 5
tttcacaaaa tgacacgttt gac                                            23


<210> 6
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> MACPF1R_R

<400> 6
tgcttaaaag atgctccttg tc                                             22


<210> 7
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> MACPF 1 Fwd

<400> 7
acatcacaca tctaagatct gc                                             22


<210> 8
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> MACPF 1 Rv

<400> 8
atggaggttt atagcgtaaa ta                                             22


<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> B. lactucae Actin Fwd

<400> 9
gcgagaaatt gtgcgtgata                                                20


<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> B. lactucae Actin Rv

<400> 10
actcggctgc agtcttcatt                                                20


<210> 11
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> LsTUA-3F

<400> 11
cttcttagtg ttcaatgctg ttgg                                           24


<210> 12
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> LsTUA-3R

<400> 12
gaagggtaga tagtgaaacc gagc                                           24


