                         SEQUENCE LISTING

<110>  The University of North Carolina at Chapel Hill
       Gray, Steven James
       Chen, Xin
 
<120>  OPTIMIZED CLN7 GENES AND EXPRESSION CASSETTES AND THEIR USE

<130>  5470-855WO

<150>  US 62/717,251
<151>  2018-08-10

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1557
<212>  DNA
<213>  Artificial

<220>
<223>  Human codon-optimized CLN7 open reading frame

<400>  1
atggccgggt tgaggaacga atccgaacag gaacccctcc tgggagacac cccaggatca       60

cgggagtggg acatcctgga aaccgaggaa cattacaagt cccggtggag gtcgatccgc      120

atcctgtacc tgacgatgtt cctgtcgtcc gtgggtttct cggtcgtgat gatgagcatc      180

tggccctacc ttcaaaagat cgacccgacc gccgatacta gcttcctggg atgggtcatc      240

gcctcctact cgctgggaca gatggtggca tcgcccatct tcggactttg gtccaactac      300

cggcccagaa aagaaccact cattgtgtct attctgattt ccgtggccgc caactgcctg      360

tacgcctacc tccacatccc cgcctcgcac aacaagtatt acatgcttgt ggccagggga      420

ctcctcggga tcggtgcagg aaacgtggct gtcgtgcgct cctacaccgc cggtgctaca      480

agcttgcaag agcgcacctc ctccatggcg aacatcagca tgtgtcaggc cctgggattc      540

atcctcggcc cggtgttcca gacatgcttc actttcctcg gcgaaaaggg cgtgacttgg      600

gacgtgatta agctgcagat caacatgtac accaccccgg tgctgctgtc agccttcctc      660

ggcattctga acatcattct gattttggcc attctgcggg agcatagagt ggatgactca      720

gggagacagt gcaaaagcat taacttcgag gaagcatcga cggacgaggc ccaagtgcca      780

cagggaaaca tcgaccaagt ggccgtcgtc gccatcaatg tgctgttttt cgtgaccctg      840

tttatcttcg ccttgttcga gactatcatt acccctctca ctatggatat gtacgcctgg      900

actcaggaac aagccgtgct gtataacgga atcatcctgg cggcgcttgg agtggaagca      960

gtggtcattt tcctcggggt caagctgctg agcaagaaga tcggtgaacg ggcgatcctc     1020

ctgggtggcc tcatcgtcgt ctgggtcggc tttttcattc tgctgccgtg gggcaaccag     1080

ttcccgaaga tccagtggga agatcttcac aacaactcca tccccaacac caccttcgga     1140

gagatcatca ttggcctgtg gaagtcccct atggaggacg acaacgaacg gcctactgga     1200

tgctccatcg aacaagcttg gtgcctctac acccccgtga tccacctggc tcagttcctg     1260

actagcgcgg tgctgatcgg tctgggttac cccgtgtgta acctgatgtc ctacaccctg     1320

tactccaaga tcctcgggcc gaagcctcag ggagtgtaca tggggtggct gactgcgagc     1380

ggatctggag cccgcattct tggcccgatg tttatctcac aagtgtacgc ccactgggga     1440

cctagatggg cgttctccct cgtgtgcggc atcattgtcc tgaccatcac cctgctggga     1500

gtggtgtaca agaggctgat cgcactgtcc gtgcgctatg ggcggattca ggaatag        1557


<210>  2
<211>  164
<212>  DNA
<213>  Artificial

<220>
<223>  JeT promoter

<400>  2
gggcggagtt agggcggagc caatcagcgt gcgccgttcc gaaagttgcc ttttatggct       60

gggcggagaa tgggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag      120

ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgt                       164


<210>  3
<211>  123
<212>  DNA
<213>  Artificial

<220>
<223>  SV40 polyadenylation signal (SV40pA)

<400>  3
tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata       60

aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc      120

atg                                                                    123


<210>  4
<211>  1890
<212>  DNA
<213>  Artificial

<220>
<223>  CLN7 expression cassette

<400>  4
ggttcggtac cgggcggagt tagggcggag ccaatcagcg tgcgccgttc cgaaagttgc       60

cttttatggc tgggcggaga atgggcggtg aacgccgatg attatataag gacgcgccgg      120

gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgttccgg      180

aaagccacca tggccgggtt gaggaacgaa tccgaacagg aacccctcct gggagacacc      240

ccaggatcac gggagtggga catcctggaa accgaggaac attacaagtc ccggtggagg      300

tcgatccgca tcctgtacct gacgatgttc ctgtcgtccg tgggtttctc ggtcgtgatg      360

atgagcatct ggccctacct tcaaaagatc gacccgaccg ccgatactag cttcctggga      420

tgggtcatcg cctcctactc gctgggacag atggtggcat cgcccatctt cggactttgg      480

tccaactacc ggcccagaaa agaaccactc attgtgtcta ttctgatttc cgtggccgcc      540

aactgcctgt acgcctacct ccacatcccc gcctcgcaca acaagtatta catgcttgtg      600

gccaggggac tcctcgggat cggtgcagga aacgtggctg tcgtgcgctc ctacaccgcc      660

ggtgctacaa gcttgcaaga gcgcacctcc tccatggcga acatcagcat gtgtcaggcc      720

ctgggattca tcctcggccc ggtgttccag acatgcttca ctttcctcgg cgaaaagggc      780

gtgacttggg acgtgattaa gctgcagatc aacatgtaca ccaccccggt gctgctgtca      840

gccttcctcg gcattctgaa catcattctg attttggcca ttctgcggga gcatagagtg      900

gatgactcag ggagacagtg caaaagcatt aacttcgagg aagcatcgac ggacgaggcc      960

caagtgccac agggaaacat cgaccaagtg gccgtcgtcg ccatcaatgt gctgtttttc     1020

gtgaccctgt ttatcttcgc cttgttcgag actatcatta cccctctcac tatggatatg     1080

tacgcctgga ctcaggaaca agccgtgctg tataacggaa tcatcctggc ggcgcttgga     1140

gtggaagcag tggtcatttt cctcggggtc aagctgctga gcaagaagat cggtgaacgg     1200

gcgatcctcc tgggtggcct catcgtcgtc tgggtcggct ttttcattct gctgccgtgg     1260

ggcaaccagt tcccgaagat ccagtgggaa gatcttcaca acaactccat ccccaacacc     1320

accttcggag agatcatcat tggcctgtgg aagtccccta tggaggacga caacgaacgg     1380

cctactggat gctccatcga acaagcttgg tgcctctaca cccccgtgat ccacctggct     1440

cagttcctga ctagcgcggt gctgatcggt ctgggttacc ccgtgtgtaa cctgatgtcc     1500

tacaccctgt actccaagat cctcgggccg aagcctcagg gagtgtacat ggggtggctg     1560

actgcgagcg gatctggagc ccgcattctt ggcccgatgt ttatctcaca agtgtacgcc     1620

cactggggac ctagatgggc gttctccctc gtgtgcggca tcattgtcct gaccatcacc     1680

ctgctgggag tggtgtacaa gaggctgatc gcactgtccg tgcgctatgg gcggattcag     1740

gaataggcct gagctctcga gtgtttattg cagcttataa tggttacaaa taaagcaata     1800

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca     1860

aactcatcaa tgtatcttat catgacgcgt                                      1890


<210>  5
<211>  519
<212>  PRT
<213>  Mus musculus

<400>  5

Met Ala Asn Leu Gly Ser Glu Ala Glu Arg Glu Pro Leu Leu Gly Pro 
1               5                   10                  15      


Gly Ser Pro Gly Ser Arg Glu Trp Ser Glu Ile Glu Thr Gln Glu His 
            20                  25                  30          


Tyr Lys Ser Arg Trp Lys Ser Val Arg Ile Leu Tyr Leu Thr Met Phe 
        35                  40                  45              


Leu Ser Ser Val Gly Phe Ser Ile Val Ile Met Ser Ile Trp Pro Tyr 
    50                  55                  60                  


Leu Gln Lys Ile Asp Gln Thr Ala Asp Ala Ser Phe Leu Gly Trp Val 
65                  70                  75                  80  


Ile Ala Ser Tyr Ser Leu Gly Gln Met Val Ala Ser Pro Leu Phe Gly 
                85                  90                  95      


Leu Trp Ser Asn Tyr Arg Pro Arg Lys Glu Pro Leu Ile Val Ser Ile 
            100                 105                 110         


Ser Ile Ser Val Ala Ala Asn Cys Leu Tyr Ala Tyr Val His Val Pro 
        115                 120                 125             


Ala Ala His Asn Lys Tyr Tyr Met Leu Ile Ala Arg Gly Leu Val Gly 
    130                 135                 140                 


Phe Gly Ala Gly Asn Val Ala Val Val Arg Ser Tyr Ile Ala Gly Ala 
145                 150                 155                 160 


Thr Ser Leu Gln Glu Arg Thr Asn Ala Met Ala Asn Thr Ser Thr Cys 
                165                 170                 175     


Gln Ala Leu Gly Phe Ile Leu Gly Pro Val Phe Gln Thr Cys Phe Ala 
            180                 185                 190         


Leu Ile Gly Glu Lys Gly Val Thr Trp Asp Ile Ile Lys Leu Gln Val 
        195                 200                 205             


Asn Met Tyr Thr Ala Pro Val Leu Leu Ala Ala Phe Leu Gly Ile Leu 
    210                 215                 220                 


Asn Ile Ile Leu Ile Leu Phe Ile Leu Arg Glu His Arg Val Asp Asp 
225                 230                 235                 240 


Leu Gly Arg Gln Cys Lys Ser Val Asn Phe Gln Glu Glu Asn Thr Asp 
                245                 250                 255     


Glu Pro Gln Ile Pro Glu Gly Ser Ile Asp Gln Val Ala Val Val Ala 
            260                 265                 270         


Thr Asn Ile Val Phe Phe Val Val Leu Phe Ile Phe Ala Val Tyr Glu 
        275                 280                 285             


Thr Ile Leu Thr Pro Leu Thr Leu Asp Met Tyr Ala Trp Thr Gln Glu 
    290                 295                 300                 


Gln Ala Val Leu Tyr Asp Gly Ile Leu Leu Val Ala Phe Gly Val Glu 
305                 310                 315                 320 


Ala Val Leu Val Phe Met Gly Val Lys Leu Leu Ser Lys Lys Ile Gly 
                325                 330                 335     


Glu Arg Ala Ile Leu Leu Gly Gly Phe Val Val Val Trp Val Gly Phe 
            340                 345                 350         


Phe Ile Leu Leu Pro Trp Gly Asn Gln Phe Pro Lys Ile Gln Trp Glu 
        355                 360                 365             


Asp Leu His Asn Ser Ser Thr Pro Asn Thr Thr Phe Gly Glu Ile Ile 
    370                 375                 380                 


Ile Gly Leu Trp Asn Ser Ser Arg Glu Asp His Ser Glu Gln Pro Thr 
385                 390                 395                 400 


Gly Cys Pro Ile Glu Gln Thr Trp Cys Leu Tyr Thr Pro Val Ile His 
                405                 410                 415     


Leu Ala Gln Phe Leu Thr Ala Ala Val Leu Ile Gly Thr Gly Tyr Pro 
            420                 425                 430         


Ala Cys Ser Val Met Ser Tyr Thr Leu Tyr Ser Lys Val Leu Gly Pro 
        435                 440                 445             


Lys Pro Gln Gly Ile Tyr Met Gly Trp Leu Thr Thr Ser Gly Ser Ala 
    450                 455                 460                 


Ala Arg Ile Leu Gly Pro Val Phe Ile Ser His Val Tyr Thr Tyr Leu 
465                 470                 475                 480 


Gly Pro Arg Trp Ala Phe Ser Leu Val Cys Gly Ile Val Val Leu Thr 
                485                 490                 495     


Ile Leu Leu Ile Gly Ala Val Tyr Lys Arg Leu Val Ala Phe Ser Val 
            500                 505                 510         


Arg Tyr Met Arg Ile Gln Glu 
        515                 


<210>  6
<211>  519
<212>  PRT
<213>  Rattus norvegicus

<400>  6

Met Ala Asn Leu Gly Ser Glu Ala Glu Arg Glu Pro Leu Leu Gly Thr 
1               5                   10                  15      


Gly Ser Pro Gly Ser Arg Glu Trp Gly Met Ile Glu Thr Gln Glu His 
            20                  25                  30          


Tyr Lys Ser Arg Trp Lys Ser Val Arg Ile Leu Tyr Leu Thr Met Phe 
        35                  40                  45              


Leu Ser Ser Val Gly Phe Ser Ile Val Ile Met Ser Ile Trp Pro Tyr 
    50                  55                  60                  


Leu Gln Lys Ile Asp Gln Thr Ala Asp Ala Ser Phe Leu Gly Trp Val 
65                  70                  75                  80  


Ile Ala Ser Tyr Ser Leu Gly Gln Met Val Ala Ser Pro Leu Phe Gly 
                85                  90                  95      


Leu Trp Ser Asn Tyr Arg Pro Arg Lys Glu Pro Leu Ile Val Ser Ile 
            100                 105                 110         


Phe Ile Ser Val Ala Ala Asn Cys Leu Tyr Ala Tyr Val His Val Pro 
        115                 120                 125             


Ala Ala His Asn Lys Tyr Tyr Met Leu Ile Ala Arg Gly Leu Val Gly 
    130                 135                 140                 


Phe Gly Ala Gly Asn Val Ala Val Val Arg Ser Tyr Ile Ala Gly Ala 
145                 150                 155                 160 


Thr Ser Leu Gln Glu Arg Thr Gly Ala Met Ala Asn Thr Ser Thr Cys 
                165                 170                 175     


Gln Ala Leu Gly Phe Ile Leu Gly Pro Val Phe Gln Thr Cys Phe Ala 
            180                 185                 190         


Leu Ile Gly Glu Lys Gly Val Ala Trp Asp Ile Ile Lys Leu Gln Ile 
        195                 200                 205             


Asn Met Tyr Thr Ala Pro Val Leu Leu Ala Ser Phe Leu Gly Ile Leu 
    210                 215                 220                 


Asn Ile Ile Leu Ile Leu Phe Ile Leu Arg Glu His Arg Val Asp Asp 
225                 230                 235                 240 


Leu Gly Arg Gln Cys Lys Ser Val Asn Val Pro Glu Glu Asn Ala Asp 
                245                 250                 255     


Glu Leu Gln Ile Pro Glu Gly Ser Ile Asp Gln Val Ala Val Val Ala 
            260                 265                 270         


Thr Asn Ile Val Phe Phe Val Val Leu Phe Ile Phe Ala Val Tyr Glu 
        275                 280                 285             


Thr Ile Leu Thr Pro Leu Thr Met Asp Met Tyr Ala Trp Thr Gln Glu 
    290                 295                 300                 


Gln Ala Val Leu Tyr Asn Gly Ile Ile Leu Val Ala Phe Gly Val Glu 
305                 310                 315                 320 


Ala Val Leu Val Phe Met Gly Val Lys Leu Leu Ser Lys Lys Ile Gly 
                325                 330                 335     


Glu Arg Ala Ile Leu Leu Gly Gly Phe Val Val Val Trp Val Gly Phe 
            340                 345                 350         


Phe Ile Leu Leu Pro Trp Gly Asn Gln Phe Pro Lys Ile Gln Trp Glu 
        355                 360                 365             


Asp Leu His Asn Ser Ser Thr Pro Asn Thr Thr Phe Gly Glu Ile Ile 
    370                 375                 380                 


Ile Asp Leu Trp Asn Ser Pro Arg Glu Asp His Ser Glu Gln Pro Thr 
385                 390                 395                 400 


Gly Cys Pro Ile Glu Gln Ala Trp Cys Leu Tyr Thr Pro Val Ile His 
                405                 410                 415     


Leu Ala Gln Phe Leu Thr Ala Ala Val Leu Val Gly Ile Gly Tyr Pro 
            420                 425                 430         


Ala Cys Ser Val Met Ser Tyr Thr Leu Tyr Ser Lys Val Leu Gly Pro 
        435                 440                 445             


Lys Pro Gln Gly Ile Tyr Met Gly Trp Leu Thr Thr Ser Gly Ser Ala 
    450                 455                 460                 


Ala Arg Ile Leu Gly Pro Val Phe Ile Ser His Val Tyr Thr Tyr Leu 
465                 470                 475                 480 


Gly Pro Arg Trp Ala Val Ser Leu Val Cys Gly Ile Val Ala Phe Thr 
                485                 490                 495     


Ile Leu Leu Ile Gly Ala Val Tyr Lys Arg Leu Val Ala Phe Ser Val 
            500                 505                 510         


Arg Tyr Gly Arg Ile Gln Glu 
        515                 


<210>  7
<211>  518
<212>  PRT
<213>  Artificial

<220>
<223>  Optimized_hMFSD8

<400>  7

Met Ala Gly Leu Arg Asn Glu Ser Glu Gln Glu Pro Leu Leu Gly Asp 
1               5                   10                  15      


Thr Pro Gly Ser Arg Glu Trp Asp Ile Leu Glu Thr Glu Glu His Tyr 
            20                  25                  30          


Lys Ser Arg Trp Arg Ser Ile Arg Ile Leu Tyr Leu Thr Met Phe Leu 
        35                  40                  45              


Ser Ser Val Gly Phe Ser Val Val Met Met Ser Ile Trp Pro Tyr Leu 
    50                  55                  60                  


Gln Lys Ile Asp Pro Thr Ala Asp Thr Ser Phe Leu Gly Trp Val Ile 
65                  70                  75                  80  


Ala Ser Tyr Ser Leu Gly Gln Met Val Ala Ser Pro Ile Phe Gly Leu 
                85                  90                  95      


Trp Ser Asn Tyr Arg Pro Arg Lys Glu Pro Leu Ile Val Ser Ile Leu 
            100                 105                 110         


Ile Ser Val Ala Ala Asn Cys Leu Tyr Ala Tyr Leu His Ile Pro Ala 
        115                 120                 125             


Ser His Asn Lys Tyr Tyr Met Leu Val Ala Arg Gly Leu Leu Gly Ile 
    130                 135                 140                 


Gly Ala Gly Asn Val Ala Val Val Arg Ser Tyr Thr Ala Gly Ala Thr 
145                 150                 155                 160 


Ser Leu Gln Glu Arg Thr Ser Ser Met Ala Asn Ile Ser Met Cys Gln 
                165                 170                 175     


Ala Leu Gly Phe Ile Leu Gly Pro Val Phe Gln Thr Cys Phe Thr Phe 
            180                 185                 190         


Leu Gly Glu Lys Gly Val Thr Trp Asp Val Ile Lys Leu Gln Ile Asn 
        195                 200                 205             


Met Tyr Thr Thr Pro Val Leu Leu Ser Ala Phe Leu Gly Ile Leu Asn 
    210                 215                 220                 


Ile Ile Leu Ile Leu Ala Ile Leu Arg Glu His Arg Val Asp Asp Ser 
225                 230                 235                 240 


Gly Arg Gln Cys Lys Ser Ile Asn Phe Glu Glu Ala Ser Thr Asp Glu 
                245                 250                 255     


Ala Gln Val Pro Gln Gly Asn Ile Asp Gln Val Ala Val Val Ala Ile 
            260                 265                 270         


Asn Val Leu Phe Phe Val Thr Leu Phe Ile Phe Ala Leu Phe Glu Thr 
        275                 280                 285             


Ile Ile Thr Pro Leu Thr Met Asp Met Tyr Ala Trp Thr Gln Glu Gln 
    290                 295                 300                 


Ala Val Leu Tyr Asn Gly Ile Ile Leu Ala Ala Leu Gly Val Glu Ala 
305                 310                 315                 320 


Val Val Ile Phe Leu Gly Val Lys Leu Leu Ser Lys Lys Ile Gly Glu 
                325                 330                 335     


Arg Ala Ile Leu Leu Gly Gly Leu Ile Val Val Trp Val Gly Phe Phe 
            340                 345                 350         


Ile Leu Leu Pro Trp Gly Asn Gln Phe Pro Lys Ile Gln Trp Glu Asp 
        355                 360                 365             


Leu His Asn Asn Ser Ile Pro Asn Thr Thr Phe Gly Glu Ile Ile Ile 
    370                 375                 380                 


Gly Leu Trp Lys Ser Pro Met Glu Asp Asp Asn Glu Arg Pro Thr Gly 
385                 390                 395                 400 


Cys Ser Ile Glu Gln Ala Trp Cys Leu Tyr Thr Pro Val Ile His Leu 
                405                 410                 415     


Ala Gln Phe Leu Thr Ser Ala Val Leu Ile Gly Leu Gly Tyr Pro Val 
            420                 425                 430         


Cys Asn Leu Met Ser Tyr Thr Leu Tyr Ser Lys Ile Leu Gly Pro Lys 
        435                 440                 445             


Pro Gln Gly Val Tyr Met Gly Trp Leu Thr Ala Ser Gly Ser Gly Ala 
    450                 455                 460                 


Arg Ile Leu Gly Pro Met Phe Ile Ser Gln Val Tyr Ala His Trp Gly 
465                 470                 475                 480 


Pro Arg Trp Ala Phe Ser Leu Val Cys Gly Ile Ile Val Leu Thr Ile 
                485                 490                 495     


Thr Leu Leu Gly Val Val Tyr Lys Arg Leu Ile Ala Leu Ser Val Arg 
            500                 505                 510         


Tyr Gly Arg Ile Gln Glu 
        515             


<210>  8
<211>  518
<212>  PRT
<213>  Macaca mulatta

<400>  8

Met Ala Asp Leu Arg Ile Glu Gly Glu Arg Glu Pro Leu Leu Gly Asp 
1               5                   10                  15      


Thr Pro Gly Ser Arg Glu Trp Asp Ile Leu Glu Thr Glu Glu His Tyr 
            20                  25                  30          


Lys Ser Arg Trp Arg Ser Ile Arg Ile Leu Tyr Leu Thr Met Phe Leu 
        35                  40                  45              


Ser Ser Val Gly Phe Ser Ile Val Ile Leu Ser Ile Trp Pro Tyr Leu 
    50                  55                  60                  


Gln Lys Ile Asp Gln Thr Ala Asp Thr Arg Phe Leu Gly Trp Val Ile 
65                  70                  75                  80  


Ala Ser Tyr Ser Leu Gly Gln Met Val Ala Ser Pro Ile Phe Gly Leu 
                85                  90                  95      


Trp Ser Asn Tyr Arg Pro Arg Lys Glu Pro Leu Val Val Ser Ile Phe 
            100                 105                 110         


Ile Ser Val Ala Ala Asn Cys Leu Tyr Ala Tyr Val His Ile Pro Ala 
        115                 120                 125             


Ser His Asn Lys Tyr Tyr Met Leu Val Ala Arg Gly Leu Leu Gly Ile 
    130                 135                 140                 


Gly Ala Gly Asn Val Ala Val Val Arg Ser Tyr Thr Ala Gly Ala Thr 
145                 150                 155                 160 


Ser Leu Gln Glu Arg Thr Ser Ser Met Ala Asn Ile Ser Met Cys Gln 
                165                 170                 175     


Ala Leu Gly Phe Ile Leu Gly Pro Val Phe Gln Thr Cys Phe Ala Phe 
            180                 185                 190         


Ile Gly Glu Lys Gly Val Thr Trp Asp Val Ile Lys Leu Arg Ile Asn 
        195                 200                 205             


Met Tyr Thr Thr Pro Val Leu Leu Ser Ala Phe Leu Gly Ile Leu Asn 
    210                 215                 220                 


Ile Ile Leu Ile Leu Ala Ile Leu Arg Glu His Arg Val Asp Asp Ser 
225                 230                 235                 240 


Gly Arg Gln Cys Lys Asn Ile Asn Phe Glu Glu Ala Ser Thr Asp Glu 
                245                 250                 255     


Ile Glu Val Pro Gln Gly Asn Ile Asp Gln Val Ala Val Val Ala Ile 
            260                 265                 270         


Asn Val Leu Phe Phe Val Ala Leu Phe Ile Phe Ala Val Phe Glu Thr 
        275                 280                 285             


Ile Ile Ala Pro Leu Thr Met Asp Met Tyr Ala Trp Thr Gln Glu Gln 
    290                 295                 300                 


Ala Val Leu Tyr Asn Gly Ile Ile Leu Ala Ala Leu Gly Val Glu Ala 
305                 310                 315                 320 


Val Val Ile Phe Val Gly Val Lys Leu Leu Ser Lys Lys Ile Gly Glu 
                325                 330                 335     


Arg Ala Ile Leu Leu Gly Gly Leu Ile Val Val Trp Val Gly Phe Phe 
            340                 345                 350         


Ile Leu Leu Pro Trp Gly Asn Gln Phe Pro Lys Ile Gln Trp Glu Asp 
        355                 360                 365             


Leu His Asn Asn Ser Ile Pro Asn Thr Thr Phe Gly Glu Ile Ile Ile 
    370                 375                 380                 


Gly Leu Trp Lys Ser Pro Met Glu Asp Asp Asn Glu Arg Pro Thr Gly 
385                 390                 395                 400 


Cys Ser Ile Glu Gln Ala Trp Cys Leu Tyr Thr Pro Val Ile His Leu 
                405                 410                 415     


Ala Gln Phe Leu Thr Ser Val Val Leu Ile Gly Val Gly Tyr Pro Val 
            420                 425                 430         


Cys Asn Leu Met Ser Tyr Thr Leu Tyr Ser Lys Val Leu Gly Pro Lys 
        435                 440                 445             


Pro Gln Gly Val Tyr Met Gly Trp Leu Thr Ala Ser Gly Ser Gly Ala 
    450                 455                 460                 


Arg Ile Leu Gly Pro Met Phe Ile Ser Gln Val Tyr Ala His Trp Gly 
465                 470                 475                 480 


Pro Arg Trp Ala Phe Ser Leu Met Cys Gly Ile Val Val Leu Thr Ile 
                485                 490                 495     


Thr Leu Leu Gly Val Val Tyr Arg Arg Leu Ile Ala Leu Ser Val Arg 
            500                 505                 510         


Tyr Gly Arg Ile Gln Glu 
        515             


