                         SEQUENCE LISTING

<110>  The General Hospital Corporation
 
<120>  Polypeptide Integrin Antagonists

<130>  29539-0423WO1

<140>  PCT/US2019/045762
<141>  2019-08-08

<150>  62/786,804
<151>  2018-12-31

<150>  62/757,126
<151>  2018-11-07

<150>  62/715,860
<151>  2018-08-08

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  91
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin

<400>  1

Ser Asp Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro Thr Ser 
1               5                   10                  15      


Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr Tyr Arg 
            20                  25                  30          


Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu Phe Thr 
        35                  40                  45              


Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro Gly 
    50                  55                  60                  


Val Asp Tyr Thr Ile Thr Val Tyr Ala Val Thr Pro Gly Asp Trp Asn 
65                  70                  75                  80  


Glu Gly Gly Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90      


<210>  2
<211>  92
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin

<400>  2

Ser Asp Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro Thr Ser 
1               5                   10                  15      


Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr Tyr Arg 
            20                  25                  30          


Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu Phe Thr 
        35                  40                  45              


Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro Gly 
    50                  55                  60                  


Val Asp Tyr Thr Ile Thr Val Tyr Ala Val Thr Pro Lys Gly Asp Trp 
65                  70                  75                  80  


Asn Glu Gly Gly Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90          


<210>  3
<211>  92
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin

<400>  3

Ser Asp Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro Thr Ser 
1               5                   10                  15      


Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr Tyr Arg 
            20                  25                  30          


Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu Phe Thr 
        35                  40                  45              


Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro Gly 
    50                  55                  60                  


Val Asp Tyr Thr Ile Thr Val Tyr Ala Val Thr Pro Arg Gly Asp Trp 
65                  70                  75                  80  


Asn Glu Gly Gly Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90          


<210>  4
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is absent of Gly

<400>  4

Xaa Lys Lys Gly Lys 
1               5   


<210>  5
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin

<400>  5

Ala Ser His His His His His His Leu Val Pro Arg Gly Ser 
1               5                   10                  


<210>  6
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin

<400>  6

Gly Lys Lys Gly Lys 
1               5   


<210>  7
<211>  91
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin

<400>  7

Ser Asp Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro Thr Ser 
1               5                   10                  15      


Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr Tyr Arg 
            20                  25                  30          


Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu Phe Thr 
        35                  40                  45              


Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro Gly 
    50                  55                  60                  


Val Asp Tyr Thr Ile Thr Val Tyr Ala Val Thr Pro Gly Asp Trp Asn 
65                  70                  75                  80  


Glu Gly Gly Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90      


<210>  8
<211>  108
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin

<400>  8

Ala Ser His His His His His His Leu Val Pro Arg Gly Ser Ser Asp 
1               5                   10                  15      


Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro Thr Ser Leu Leu 
            20                  25                  30          


Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr Tyr Arg Ile Thr 
        35                  40                  45              


Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu Phe Thr Val Pro 
    50                  55                  60                  


Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro Gly Val Asp 
65                  70                  75                  80  


Tyr Thr Ile Thr Val Tyr Ala Val Thr Pro His Ala Arg Gly Asp Trp 
                85                  90                  95      


Asn Glu Gly Gly Pro Ile Ser Ile Asn Tyr Arg Thr 
            100                 105             


<210>  9
<211>  110
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin

<400>  9

Ala Ser His His His His His His Leu Val Pro Arg Gly Ser Ser Asp 
1               5                   10                  15      


Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro Thr Ser Leu Leu 
            20                  25                  30          


Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr Tyr Arg Ile Thr 
        35                  40                  45              


Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu Phe Thr Val Pro 
    50                  55                  60                  


Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro Gly Val Asp 
65                  70                  75                  80  


Tyr Thr Ile Thr Val Tyr Ala Val Thr Pro Gly Asp Trp Asn Glu Gly 
                85                  90                  95      


Gly Pro Ile Ser Ile Asn Tyr Arg Thr Gly Lys Lys Gly Lys 
            100                 105                 110 


<210>  10
<211>  92
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin

<400>  10

Ser Asp Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro Thr Ser 
1               5                   10                  15      


Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr Tyr Arg 
            20                  25                  30          


Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu Phe Thr 
        35                  40                  45              


Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro Gly 
    50                  55                  60                  


Val Asp Tyr Thr Ile Thr Val Tyr Ala Val Thr Pro Lys Gly Asp Trp 
65                  70                  75                  80  


Asn Glu Gly Gly Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90          


<210>  11
<211>  92
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin

<400>  11

Ser Asp Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro Thr Ser 
1               5                   10                  15      


Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr Tyr Arg 
            20                  25                  30          


Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu Phe Thr 
        35                  40                  45              


Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro Gly 
    50                  55                  60                  


Val Asp Tyr Thr Ile Thr Val Tyr Ala Val Thr Pro Arg Gly Asp Trp 
65                  70                  75                  80  


Asn Glu Gly Gly Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90          


<210>  12
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA target sequence

<400>  12
cttgagctca aggtaggcac                                                   20


<210>  13
<211>  120
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide that binds integrin

<400>  13

Ala Cys Ala Thr Cys Thr Cys Thr Cys Ala Gly Ala Ala Gly Cys Gly 
1               5                   10                  15      


Cys Ala Thr Cys Cys Gly Cys Gly Thr Gly Gly Cys Ala Gly Thr Gly 
            20                  25                  30          


Gly Thr Ala Gly Ala Gly Thr Ala Cys Cys Ala Thr Gly Ala Thr Gly 
        35                  40                  45              


Gly Ala Thr Cys Cys Cys Ala Thr Gly Cys Thr Thr Ala Thr Cys Thr 
    50                  55                  60                  


Thr Gly Ala Gly Cys Thr Cys Ala Ala Gly Gly Cys Cys Cys Gly Gly 
65                  70                  75                  80  


Ala Ala Gly Cys Gly Ala Cys Cys Cys Thr Cys Ala Gly Ala Gly Cys 
                85                  90                  95      


Thr Thr Cys Gly Gly Cys Gly Cys Ala Thr Cys Ala Cys Cys Ala Gly 
            100                 105                 110         


Cys Cys Ala Gly Ala Thr Thr Ala 
        115                 120 


<210>  14
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  primer sequence for PCR genotyping

<400>  14

Thr Cys Ala Cys Thr Gly Thr Gly Ala Thr Gly Gly Thr Gly Thr Gly 
1               5                   10                  15      


Ala Ala Cys Cys 
            20  


<210>  15
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  primer sequence for PCR genotyping

<400>  15

Cys Thr Gly Ala Cys Thr Ala Thr Cys Thr Cys Ala Thr Cys Thr Cys 
1               5                   10                  15      


Thr Thr Cys 
            


<210>  16
<211>  238
<212>  PRT
<213>  Homo sapiens

<400>  16

Gly Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Ala Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys His His His His His His 
225                 230                 235             


<210>  17
<211>  87
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist

<400>  17

Gly Ser Ser Asp Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro 
1               5                   10                  15      


Thr Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr 
            20                  25                  30          


Tyr Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu 
        35                  40                  45              


Phe Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Ile Ser Ile Asn Tyr 
65                  70                  75                  80  


Arg Thr Gly Lys Lys Gly Lys 
                85          


<210>  18
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist


<220>
<221>  misc_feature
<222>  1, 2, 3, 11, 12, 13
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  4
<223>  Xaa can be Arg, Ile and Leu

<220>
<221>  misc_feature
<222>  5
<223>  Xaa can be Gly and Asp

<220>
<221>  misc_feature
<222>  6
<223>  Xaa can be Asp, Ser and Thr

<220>
<221>  misc_feature
<222>  7
<223>  Xaa can be Leu and Trp

<220>
<221>  misc_feature
<222>  8
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  9
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  10
<223>  Xaa can be can be Leu and Ile

<400>  18

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10              


<210>  19
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist


<220>
<221>  misc_feature
<222>  1, 2, 3, 11, 12, 13
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  8
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  9
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  10
<223>  Xaa can be can be Leu and Ile

<400>  19

Xaa Xaa Xaa Arg Gly Asp Leu Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10              


<210>  20
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist


<220>
<221>  misc_feature
<222>  1, 2, 3, 8, 9, 10
<223>  Xaa can be any naturally occurring amino acid

<400>  20

Xaa Xaa Xaa Arg Gly Asp Trp Xaa Xaa Xaa 
1               5                   10  


<210>  21
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist


<220>
<221>  misc_feature
<222>  1, 2, 3, 8, 9, 10
<223>  Xaa can be any naturally occurring amino acid


<400>  21

Xaa Xaa Xaa Arg Gly Asp Trp Xaa Xaa Xaa 
1               5                   10  


<210>  22
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist


<220>
<221>  misc_feature
<222>  1, 2, 3, 7, 8, 9
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  4
<223>  Xaa can be Arg, Ile and Leu

<220>
<221>  misc_feature
<222>  6
<223>  Xaa can be Asp, Ser and Thr

<400>  22

Xaa Xaa Xaa Xaa Asp Xaa Xaa Xaa Xaa 
1               5                   


<210>  23
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist


<220>
<221>  misc_feature
<222>  1, 2, 3, 11, 12, 13
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  8, 9
<223>  Xaa can be any naturally occurring amino acid


<400>  23

Xaa Xaa Xaa Arg Gly Asp Leu Xaa Xaa Leu Xaa Xaa Xaa 
1               5                   10              


<210>  24
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist


<220>
<221>  misc_feature
<222>  1, 2, 3, 11, 12, 13
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  8, 9
<223>  Xaa can be any naturally occurring amino acid


<400>  24

Xaa Xaa Xaa Arg Gly Asp Leu Xaa Xaa Ile Xaa Xaa Xaa 
1               5                   10              


<210>  25
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist


<220>
<221>  misc_feature
<222>  1, 2, 3, 7, 8, 9
<223>  Xaa can be any naturally occurring amino acid


<400>  25

Xaa Xaa Xaa Ile Asp Ser Xaa Xaa Xaa 
1               5                   


<210>  26
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  XXXIDTXXX


<220>
<221>  misc_feature
<222>  1, 2, 3, 7, 8, 9
<223>  Xaa can be any naturally occurring amino acid


<400>  26

Xaa Xaa Xaa Ile Asp Thr Xaa Xaa Xaa 
1               5                   


<210>  27
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist


<220>
<221>  misc_feature
<222>  1, 2, 3, 7, 8, 9
<223>  Xaa can be any naturally occurring amino acid


<400>  27

Xaa Xaa Xaa Leu Asp Ser Xaa Xaa Xaa 
1               5                   


<210>  28
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist


<220>
<221>  misc_feature
<222>  1, 2, 3, 7, 8, 9
<223>  Xaa can be any naturally occurring amino acid


<400>  28

Xaa Xaa Xaa Leu Asp Thr Xaa Xaa Xaa 
1               5                   


<210>  29
<211>  90
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide for identifying the pure integrin 
       antagonist

<400>  29

Gly Ser Ser Asp Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro 
1               5                   10                  15      


Thr Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr 
            20                  25                  30          


Tyr Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu 
        35                  40                  45              


Phe Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Gly Gly Pro Ile Ser 
65                  70                  75                  80  


Ile Asn Tyr Arg Thr Gly Lys Lys Gly Lys 
                85                  90  


<210>  30
<211>  90
<212>  PRT
<213>  Artificial

<220>
<223>  polypeptide for identifying the pure integrin antagonist

<400>  30

Gly Ser Ser Asp Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro 
1               5                   10                  15      


Thr Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr 
            20                  25                  30          


Tyr Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu 
        35                  40                  45              


Phe Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Gly Gly Pro Ile Ser 
65                  70                  75                  80  


Ile Asn Tyr Arg Thr Gly Lys Lys Gly Lys 
                85                  90  


