                         SEQUENCE LISTING

<110>  NemaMetrix Inc.
 
<120>  HOMOLOGOUS RECOMBINATION REPORTER CONSTRUCT AND USES THEREOF

<130>  NEMA005PCT

<150>  US 62/715,117
<151>  2018-08-06

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  869
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C. elegans codon-optimized mCherry sequence

<400>  1
atggtctcca agggagagga ggacaacatg gccatcatca aggagttcat gcgtttcaag       60

gtccacatgg agggatccgt caacggacac gagttcgaga tcgagggaga gggagaggga      120

cgtccatacg agggaaccca aaccgccaag ctcaaggtca ccaaggtaag tttaaacata      180

tatatactaa ctaaggaggc cctgattatt taaattttca gggaggaccc ctcccttttg      240

cttgggatat tctttccccc caattcatgt acggatctaa agcctacgtc aagcacccag      300

ccgacatccc agactacctc aagctctcct tcccagaggg attcaagtgg gagcgtgtca      360

tgaacttcga ggacggagga gtcgtcaccg tcacccaaga ctcctccctc caagacggag      420

agttcatcta taaggtaagt ttaaacagtt cggcgcgccc taaccataca tatttaaatt      480

ttcaggtcaa gctccgtgga accaacttcc catccgacgg accagtcatg caaaagaaga      540

ccatgggatg ggaggcctcc tccgagcgta tgtacccaga ggacggagcc ctcaagggag      600

agatcaagca acgtctcaag ctcaaggtaa gtttaaacat gattttacta actaactaat      660

ctgatttaaa ttttcaggac ggaggacact acgacgccga ggtcaagacc acctacaagg      720

ccaagaagcc agtccaactc ccaggagcct acaacgtcaa catcaagctc gacatcacct      780

cccacaacga ggactacacc atcgtcgagc aatacgagcg tgccgaggga cgtcactcca      840

ccggaggaat ggacgagctc tacaagtaa                                        869


<210>  2
<211>  573
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eft-3 gene promoter

<400>  2
tgtttctgtt aaattaatga atttttcata aaataaagac attatacaat ataaaaatga       60

agaatttatt gaaaataaac tgccagagag aaaaagtatg caacactccc gccgagagtg      120

tttgaaatgg tgtacggtac attttcgtgc taggagttag atgtgcaggc agcaacgaga      180

gggggagaga tttttttggg ccttgtgaaa ttaacgtgag ttttctggtc atctgactaa      240

tcatgttggt tttttgttgg tttattttgt ttttatcttt gtttttatcc agattaggaa      300

atttaaattt tatgaattta taatgaggtc aaacattcag tcccagcgtt tttcctgttc      360

tcactgttta gtcgaatttt tattttaggc tttcaacaaa tgttctaact gtcttatttg      420

tgacctcact ttttatattt ttttaatttt taaaaatatt agaagtttct aggataattt      480

tttcgacttt tattctctct accgtccgca ctcttcttac ttttaaatta aattgttttt      540

ttttcagttg ggaaacactt tgctcactcc gta                                   573


<210>  3
<211>  708
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zebrafish codon optimized coding sequence for mCherry

<400>  3
atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag       60

gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc      120

cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc      180

ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac      240

cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc      300

gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac      360

ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta      420

atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc      480

gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct      540

gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc      600

aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa      660

cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaag                   708


<210>  4
<211>  1020
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rpl13a promoter sequence

<400>  4
ggtgcatttg gcaagaaaca ggccgctgag gaggagatgt acttcaagtg agtggttttg       60

cttgagctga taattatgta attgcttata cttgatatct actggccatt agctgagtat      120

tattgaaaaa ataactgaat gtaaagcaac ctaaaccgtt acttcatgac ctattctgtc      180

attgtatttc cttcacagga gaaaagagca agaacagctg tctgctctga gaagacacca      240

ccaggaagag attgaccatc acaagaagga aatcgagaga ttacagcatg agatcacccg      300

ccacgagagc aaaatcaaga aactcaaaca tgatgactga ggcattaaga cagaaaatac      360

aacacatgaa ttgtgaaact gctgaatatt tgtaattgct tatttactaa acagtgaact      420

ctgtgattat actattataa aagcatgtta taatacagat atggttatat aactgaaaca      480

acacattgtg tattaaccca gtgcattttc cctcttttga caataaacaa gaaattgtct      540

cgaatgtaaa agtgtgtctt ggtatcaata cgtttggtga aagctaacta ttagctaaac      600

taactaaagc taactattgg tttgagagct aaatgtatct taactgttac tttcagtcat      660

ataaataggt tatgtcatct gaccagacaa ttaaaggttc tgacaccaat gaatgaccca      720

atattgtata aatatgagat atattaaaat atgccgtaat gctgggtttc aggatcagat      780

tgagaaacac tgctttagaa aatgttcgag acaacacttc tttattatta tatttttaat      840

attttaaagg cgttgtagct cattggagcc cagctgatgg cagtagacat aaataacagg      900

cattacaaac gtcctctgaa gaacagctaa tcctaacgtc atttccgatg acgcgaaagc      960

tccgccctcg cccctgtctt ttacgccagg cggccccgcg tgtctttctt ttcccacatc     1020


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  STXBP1 S42P target sgRNA

<400>  5
tagtggacca gctcagcatg                                                   20


<210>  6
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  STXBP1 S42P repair DNA

<400>  6
gccctctgtc atgatatcag tcattttgca gcaggaaggc agcatgcgca tgctgagctg       60

gtccactatc aaagcctaca gagagaa                                           87


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  STXBP1 S42P HR reporter sgRNA

<400>  7
gctaccatag gcaccacgag                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sgRNA #1

<400>  8
ttgcagttga acgaagaagg                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sgRNA #2

<400>  9
atgacagaat taaggagctg                                                   20


<210>  10
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  repair DNA

<400>  10
aatgtctcgt ttttttttca tccttgcagt tgaacgaaga agacgattca acatcaacga       60

taggatcaaa gaactgggga ctttaattcc caagtcaaat gatccgtaag ttt             113


