                         SEQUENCE LISTING

<110>  The University of Greenwich
 
<120>  Liposomes

<130>  88170PCT1

<150>  GB 1812992.4
<151>  2018-08-09

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  747
<212>  PRT
<213>  Bacillus anthracis

<400>  1

Met Arg Gly Ser His His His His His His Gly Ser Glu Val Lys Gln 
1               5                   10                  15      


Glu Asn Arg Leu Leu Asn Glu Ser Glu Ser Ser Ser Gln Gly Leu Leu 
            20                  25                  30          


Gly Tyr Tyr Phe Ser Asp Leu Asn Phe Gln Ala Pro Met Val Val Thr 
        35                  40                  45              


Ser Ser Thr Thr Gly Asp Leu Ser Ile Pro Ser Ser Glu Leu Glu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Asn Gln Tyr Phe Gln Ser Ala Ile Trp Ser Gly Phe 
65                  70                  75                  80  


Ile Lys Val Lys Lys Ser Asp Glu Tyr Thr Phe Ala Thr Ser Ala Asp 
                85                  90                  95      


Asn His Val Thr Met Trp Val Asp Asp Gln Glu Val Ile Asn Lys Ala 
            100                 105                 110         


Ser Asn Ser Asn Lys Ile Arg Leu Glu Lys Gly Arg Leu Tyr Gln Ile 
        115                 120                 125             


Lys Ile Gln Tyr Gln Arg Glu Asn Pro Thr Glu Lys Gly Leu Asp Phe 
    130                 135                 140                 


Lys Leu Tyr Trp Thr Asp Ser Gln Asn Lys Lys Glu Val Ile Ser Ser 
145                 150                 155                 160 


Asp Asn Leu Gln Leu Pro Glu Leu Lys Gln Lys Ser Ser Asn Ser Arg 
                165                 170                 175     


Lys Lys Arg Ser Thr Ser Ala Gly Pro Thr Val Pro Asp Arg Asp Asn 
            180                 185                 190         


Asp Gly Ile Pro Asp Ser Leu Glu Val Glu Gly Tyr Thr Val Asp Val 
        195                 200                 205             


Lys Asn Lys Arg Thr Phe Leu Ser Pro Trp Ile Ser Asn Ile His Glu 
    210                 215                 220                 


Lys Lys Gly Leu Thr Lys Tyr Lys Ser Ser Pro Glu Lys Trp Ser Thr 
225                 230                 235                 240 


Ala Ser Asp Pro Tyr Ser Asp Phe Glu Lys Val Thr Gly Arg Ile Asp 
                245                 250                 255     


Lys Asn Val Ser Pro Glu Ala Arg His Pro Leu Val Ala Ala Tyr Pro 
            260                 265                 270         


Ile Val His Val Asp Met Glu Asn Ile Ile Leu Ser Lys Asn Glu Asp 
        275                 280                 285             


Gln Ser Thr Gln Asn Thr Asp Ser Gln Thr Arg Thr Ile Ser Lys Asn 
    290                 295                 300                 


Thr Ser Thr Ser Arg Thr His Thr Ser Glu Val His Gly Asn Ala Glu 
305                 310                 315                 320 


Val His Ala Ser Phe Phe Asp Ile Gly Gly Ser Val Ser Ala Gly Phe 
                325                 330                 335     


Ser Asn Ser Asn Ser Ser Thr Val Ala Ile Asp His Ser Leu Ser Leu 
            340                 345                 350         


Ala Gly Glu Arg Thr Trp Ala Glu Thr Met Gly Leu Asn Thr Ala Asp 
        355                 360                 365             


Thr Ala Arg Leu Asn Ala Asn Ile Arg Tyr Val Asn Thr Gly Thr Ala 
    370                 375                 380                 


Pro Ile Tyr Asn Val Leu Pro Thr Thr Ser Leu Val Leu Gly Lys Asn 
385                 390                 395                 400 


Gln Thr Leu Ala Thr Ile Lys Ala Lys Glu Asn Gln Leu Ser Gln Ile 
                405                 410                 415     


Leu Ala Pro Asn Asn Tyr Tyr Pro Ser Lys Asn Leu Ala Pro Ile Ala 
            420                 425                 430         


Leu Asn Ala Gln Asp Asp Phe Ser Ser Thr Pro Ile Thr Met Asn Tyr 
        435                 440                 445             


Asn Gln Phe Leu Glu Leu Glu Lys Thr Lys Gln Leu Arg Leu Asp Thr 
    450                 455                 460                 


Asp Gln Val Tyr Gly Asn Ile Ala Thr Tyr Asn Phe Glu Asn Gly Arg 
465                 470                 475                 480 


Val Arg Val Asp Thr Gly Ser Asn Trp Ser Glu Val Leu Pro Gln Ile 
                485                 490                 495     


Gln Glu Thr Thr Ala Arg Ile Ile Phe Asn Gly Lys Asp Leu Asn Leu 
            500                 505                 510         


Val Glu Arg Arg Ile Ala Ala Val Asn Pro Ser Asp Pro Leu Glu Thr 
        515                 520                 525             


Thr Lys Pro Asp Met Thr Leu Lys Glu Ala Leu Lys Ile Ala Phe Gly 
    530                 535                 540                 


Phe Asn Glu Pro Asn Gly Asn Leu Gln Tyr Gln Gly Lys Asp Ile Thr 
545                 550                 555                 560 


Glu Phe Asp Phe Asn Phe Asp Gln Gln Thr Ser Gln Asn Ile Lys Asn 
                565                 570                 575     


Gln Leu Ala Glu Leu Asn Ala Thr Asn Ile Tyr Thr Val Leu Asp Lys 
            580                 585                 590         


Ile Lys Leu Asn Ala Lys Met Asn Ile Leu Ile Arg Asp Lys Arg Phe 
        595                 600                 605             


His Tyr Asp Arg Asn Asn Ile Ala Val Gly Ala Asp Glu Ser Val Val 
    610                 615                 620                 


Lys Glu Ala His Arg Glu Val Ile Asn Ser Ser Thr Glu Gly Leu Leu 
625                 630                 635                 640 


Leu Asn Ile Asp Lys Asp Ile Arg Lys Ile Leu Ser Gly Tyr Ile Val 
                645                 650                 655     


Glu Ile Glu Asp Thr Glu Gly Leu Lys Glu Val Ile Asn Asp Arg Tyr 
            660                 665                 670         


Asp Met Leu Asn Ile Ser Ser Leu Arg Gln Asp Gly Lys Thr Phe Ile 
        675                 680                 685             


Asp Phe Lys Lys Tyr Asn Asp Lys Leu Pro Leu Tyr Ile Ser Asn Pro 
    690                 695                 700                 


Asn Tyr Lys Val Asn Val Tyr Ala Val Thr Lys Glu Asn Thr Ile Ile 
705                 710                 715                 720 


Asn Pro Ser Glu Asn Gly Asp Thr Ser Thr Asn Gly Ile Lys Lys Ile 
                725                 730                 735     


Leu Ile Phe Ser Lys Lys Gly Tyr Glu Ile Gly 
            740                 745         


<210>  2
<211>  747
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MRSG-6His-PA83

<400>  2

Met Arg Gly Ser His His His His His His Gly Ser Glu Val Lys Gln 
1               5                   10                  15      


Glu Asn Arg Leu Leu Asn Glu Ser Glu Ser Ser Ser Gln Gly Leu Leu 
            20                  25                  30          


Gly Tyr Tyr Phe Ser Asp Leu Asn Phe Gln Ala Pro Met Val Val Thr 
        35                  40                  45              


Ser Ser Thr Thr Gly Asp Leu Ser Ile Pro Ser Ser Glu Leu Glu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Asn Gln Tyr Phe Gln Ser Ala Ile Trp Ser Gly Phe 
65                  70                  75                  80  


Ile Lys Val Lys Lys Ser Asp Glu Tyr Thr Phe Ala Thr Ser Ala Asp 
                85                  90                  95      


Asn His Val Thr Met Trp Val Asp Asp Gln Glu Val Ile Asn Lys Ala 
            100                 105                 110         


Ser Asn Ser Asn Lys Ile Arg Leu Glu Lys Gly Arg Leu Tyr Gln Ile 
        115                 120                 125             


Lys Ile Gln Tyr Gln Arg Glu Asn Pro Thr Glu Lys Gly Leu Asp Phe 
    130                 135                 140                 


Lys Leu Tyr Trp Thr Asp Ser Gln Asn Lys Lys Glu Val Ile Ser Ser 
145                 150                 155                 160 


Asp Asn Leu Gln Leu Pro Glu Leu Lys Gln Lys Ser Ser Asn Ser Arg 
                165                 170                 175     


Lys Lys Arg Ser Thr Ser Ala Gly Pro Thr Val Pro Asp Arg Asp Asn 
            180                 185                 190         


Asp Gly Ile Pro Asp Ser Leu Glu Val Glu Gly Tyr Thr Val Asp Val 
        195                 200                 205             


Lys Asn Lys Arg Thr Phe Leu Ser Pro Trp Ile Ser Asn Ile His Glu 
    210                 215                 220                 


Lys Lys Gly Leu Thr Lys Tyr Lys Ser Ser Pro Glu Lys Trp Ser Thr 
225                 230                 235                 240 


Ala Ser Asp Pro Tyr Ser Asp Phe Glu Lys Val Thr Gly Arg Ile Asp 
                245                 250                 255     


Lys Asn Val Ser Pro Glu Ala Arg His Pro Leu Val Ala Ala Tyr Pro 
            260                 265                 270         


Ile Val His Val Asp Met Glu Asn Ile Ile Leu Ser Lys Asn Glu Asp 
        275                 280                 285             


Gln Ser Thr Gln Asn Thr Asp Ser Gln Thr Arg Thr Ile Ser Lys Asn 
    290                 295                 300                 


Thr Ser Thr Ser Arg Thr His Thr Ser Glu Val His Gly Asn Ala Glu 
305                 310                 315                 320 


Val His Ala Ser Phe Phe Asp Ile Gly Gly Ser Val Ser Ala Gly Phe 
                325                 330                 335     


Ser Asn Ser Asn Ser Ser Thr Val Ala Ile Asp His Ser Leu Ser Leu 
            340                 345                 350         


Ala Gly Glu Arg Thr Trp Ala Glu Thr Met Gly Leu Asn Thr Ala Asp 
        355                 360                 365             


Thr Ala Arg Leu Asn Ala Asn Ile Arg Tyr Val Asn Thr Gly Thr Ala 
    370                 375                 380                 


Pro Ile Tyr Asn Val Leu Pro Thr Thr Ser Leu Val Leu Gly Lys Asn 
385                 390                 395                 400 


Gln Thr Leu Ala Thr Ile Lys Ala Lys Glu Asn Gln Leu Ser Gln Ile 
                405                 410                 415     


Leu Ala Pro Asn Asn Tyr Tyr Pro Ser Lys Asn Leu Ala Pro Ile Ala 
            420                 425                 430         


Leu Asn Ala Gln Asp Asp Phe Ser Ser Thr Pro Ile Thr Met Asn Tyr 
        435                 440                 445             


Asn Gln Phe Leu Glu Leu Glu Lys Thr Lys Gln Leu Arg Leu Asp Thr 
    450                 455                 460                 


Asp Gln Val Tyr Gly Asn Ile Ala Thr Tyr Asn Phe Glu Asn Gly Arg 
465                 470                 475                 480 


Val Arg Val Asp Thr Gly Ser Asn Trp Ser Glu Val Leu Pro Gln Ile 
                485                 490                 495     


Gln Glu Thr Thr Ala Arg Ile Ile Phe Asn Gly Lys Asp Leu Asn Leu 
            500                 505                 510         


Val Glu Arg Arg Ile Ala Ala Val Asn Pro Ser Asp Pro Leu Glu Thr 
        515                 520                 525             


Thr Lys Pro Asp Met Thr Leu Lys Glu Ala Leu Lys Ile Ala Phe Gly 
    530                 535                 540                 


Phe Asn Glu Pro Asn Gly Asn Leu Gln Tyr Gln Gly Lys Asp Ile Thr 
545                 550                 555                 560 


Glu Phe Asp Phe Asn Phe Asp Gln Gln Thr Ser Gln Asn Ile Lys Asn 
                565                 570                 575     


Gln Leu Ala Glu Leu Asn Ala Thr Asn Ile Tyr Thr Val Leu Asp Lys 
            580                 585                 590         


Ile Lys Leu Asn Ala Lys Met Asn Ile Leu Ile Arg Asp Lys Arg Phe 
        595                 600                 605             


His Tyr Asp Arg Asn Asn Ile Ala Val Gly Ala Asp Glu Ser Val Val 
    610                 615                 620                 


Lys Glu Ala His Arg Glu Val Ile Asn Ser Ser Thr Glu Gly Leu Leu 
625                 630                 635                 640 


Leu Asn Ile Asp Lys Asp Ile Arg Lys Ile Leu Ser Gly Tyr Ile Val 
                645                 650                 655     


Glu Ile Glu Asp Thr Glu Gly Leu Lys Glu Val Ile Asn Asp Arg Tyr 
            660                 665                 670         


Asp Met Leu Asn Ile Ser Ser Leu Arg Gln Asp Gly Lys Thr Phe Ile 
        675                 680                 685             


Asp Phe Lys Lys Tyr Asn Asp Lys Leu Pro Leu Tyr Ile Ser Asn Pro 
    690                 695                 700                 


Asn Tyr Lys Val Asn Val Tyr Ala Val Thr Lys Glu Asn Thr Ile Ile 
705                 710                 715                 720 


Asn Pro Ser Glu Asn Gly Asp Thr Ser Thr Asn Gly Ile Lys Lys Ile 
                725                 730                 735     


Leu Ile Phe Ser Lys Lys Gly Tyr Glu Ile Gly 
            740                 745         


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MRSG-6H

<400>  3

Met Arg Gly Ser His His His His His His 
1               5                   10  


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6His

<400>  4

His His His His His His 
1               5       


<210>  5
<211>  568
<212>  PRT
<213>  Bacillus anthracis

<400>  5

Ser Thr Ser Ala Gly Pro Thr Val Pro Asp Arg Asp Asn Asp Gly Ile 
1               5                   10                  15      


Pro Asp Ser Leu Glu Val Glu Gly Tyr Thr Val Asp Val Lys Asn Lys 
            20                  25                  30          


Arg Thr Phe Leu Ser Pro Trp Ile Ser Asn Ile His Glu Lys Lys Gly 
        35                  40                  45              


Leu Thr Lys Tyr Lys Ser Ser Pro Glu Lys Trp Ser Thr Ala Ser Asp 
    50                  55                  60                  


Pro Tyr Ser Asp Phe Glu Lys Val Thr Gly Arg Ile Asp Lys Asn Val 
65                  70                  75                  80  


Ser Pro Glu Ala Arg His Pro Leu Val Ala Ala Tyr Pro Ile Val His 
                85                  90                  95      


Val Asp Met Glu Asn Ile Ile Leu Ser Lys Asn Glu Asp Gln Ser Thr 
            100                 105                 110         


Gln Asn Thr Asp Ser Gln Thr Arg Thr Ile Ser Lys Asn Thr Ser Thr 
        115                 120                 125             


Ser Arg Thr His Thr Ser Glu Val His Gly Asn Ala Glu Val His Ala 
    130                 135                 140                 


Ser Phe Phe Asp Ile Gly Gly Ser Val Ser Ala Gly Phe Ser Asn Ser 
145                 150                 155                 160 


Asn Ser Ser Thr Val Ala Ile Asp His Ser Leu Ser Leu Ala Gly Glu 
                165                 170                 175     


Arg Thr Trp Ala Glu Thr Met Gly Leu Asn Thr Ala Asp Thr Ala Arg 
            180                 185                 190         


Leu Asn Ala Asn Ile Arg Tyr Val Asn Thr Gly Thr Ala Pro Ile Tyr 
        195                 200                 205             


Asn Val Leu Pro Thr Thr Ser Leu Val Leu Gly Lys Asn Gln Thr Leu 
    210                 215                 220                 


Ala Thr Ile Lys Ala Lys Glu Asn Gln Leu Ser Gln Ile Leu Ala Pro 
225                 230                 235                 240 


Asn Asn Tyr Tyr Pro Ser Lys Asn Leu Ala Pro Ile Ala Leu Asn Ala 
                245                 250                 255     


Gln Asp Asp Phe Ser Ser Thr Pro Ile Thr Met Asn Tyr Asn Gln Phe 
            260                 265                 270         


Leu Glu Leu Glu Lys Thr Lys Gln Leu Arg Leu Asp Thr Asp Gln Val 
        275                 280                 285             


Tyr Gly Asn Ile Ala Thr Tyr Asn Phe Glu Asn Gly Arg Val Arg Val 
    290                 295                 300                 


Asp Thr Gly Ser Asn Trp Ser Glu Val Leu Pro Gln Ile Gln Glu Thr 
305                 310                 315                 320 


Thr Ala Arg Ile Ile Phe Asn Gly Lys Asp Leu Asn Leu Val Glu Arg 
                325                 330                 335     


Arg Ile Ala Ala Val Asn Pro Ser Asp Pro Leu Glu Thr Thr Lys Pro 
            340                 345                 350         


Asp Met Thr Leu Lys Glu Ala Leu Lys Ile Ala Phe Gly Phe Asn Glu 
        355                 360                 365             


Pro Asn Gly Asn Leu Gln Tyr Gln Gly Lys Asp Ile Thr Glu Phe Asp 
    370                 375                 380                 


Phe Asn Phe Asp Gln Gln Thr Ser Gln Asn Ile Lys Asn Gln Leu Ala 
385                 390                 395                 400 


Glu Leu Asn Ala Thr Asn Ile Tyr Thr Val Leu Asp Lys Ile Lys Leu 
                405                 410                 415     


Asn Ala Lys Met Asn Ile Leu Ile Arg Asp Lys Arg Phe His Tyr Asp 
            420                 425                 430         


Arg Asn Asn Ile Ala Val Gly Ala Asp Glu Ser Val Val Lys Glu Ala 
        435                 440                 445             


His Arg Glu Val Ile Asn Ser Ser Thr Glu Gly Leu Leu Leu Asn Ile 
    450                 455                 460                 


Asp Lys Asp Ile Arg Lys Ile Leu Ser Gly Tyr Ile Val Glu Ile Glu 
465                 470                 475                 480 


Asp Thr Glu Gly Leu Lys Glu Val Ile Asn Asp Arg Tyr Asp Met Leu 
                485                 490                 495     


Asn Ile Ser Ser Leu Arg Gln Asp Gly Lys Thr Phe Ile Asp Phe Lys 
            500                 505                 510         


Lys Tyr Asn Asp Lys Leu Pro Leu Tyr Ile Ser Asn Pro Asn Tyr Lys 
        515                 520                 525             


Val Asn Val Tyr Ala Val Thr Lys Glu Asn Thr Ile Ile Asn Pro Ser 
    530                 535                 540                 


Glu Asn Gly Asp Thr Ser Thr Asn Gly Ile Lys Lys Ile Leu Ile Phe 
545                 550                 555                 560 


Ser Lys Lys Gly Tyr Glu Ile Gly 
                565             


<210>  6
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fragment of B. anthracis PA63

<400>  6

Val His Val Asp Met Glu Asn Ile Ile Leu Ser Lys Asn Glu Asp Gln 
1               5                   10                  15      


Ser Thr Gln Asn Thr Asp Ser Gln Thr Arg Thr Ile Ser Lys Asn Thr 
            20                  25                  30          


Ser Thr Ser Arg Thr His Thr Ser Glu Val His Gly Asn Ala Glu Val 
        35                  40                  45              


His Ala Ser Phe Phe Asp Ile Gly Gly Ser Val Ser Ala Gly Phe Ser 
    50                  55                  60                  


Asn Ser Asn Ser Ser Thr Val Ala Ile Asp His Ser Leu Ser Leu Ala 
65                  70                  75                  80  


Gly Glu Arg Thr Trp Ala Glu Thr Met Gly Leu Asn Thr Ala Asp Thr 
                85                  90                  95      


Ala Arg Leu Asn Ala Asn Ile Arg Tyr Val Asn Thr 
            100                 105             


<210>  7
<211>  747
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  b. anthracis PA83 D512K mutant

<400>  7

Met Arg Gly Ser His His His His His His Gly Ser Glu Val Lys Gln 
1               5                   10                  15      


Glu Asn Arg Leu Leu Asn Glu Ser Glu Ser Ser Ser Gln Gly Leu Leu 
            20                  25                  30          


Gly Tyr Tyr Phe Ser Asp Leu Asn Phe Gln Ala Pro Met Val Val Thr 
        35                  40                  45              


Ser Ser Thr Thr Gly Asp Leu Ser Ile Pro Ser Ser Glu Leu Glu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Asn Gln Tyr Phe Gln Ser Ala Ile Trp Ser Gly Phe 
65                  70                  75                  80  


Ile Lys Val Lys Lys Ser Asp Glu Tyr Thr Phe Ala Thr Ser Ala Asp 
                85                  90                  95      


Asn His Val Thr Met Trp Val Asp Asp Gln Glu Val Ile Asn Lys Ala 
            100                 105                 110         


Ser Asn Ser Asn Lys Ile Arg Leu Glu Lys Gly Arg Leu Tyr Gln Ile 
        115                 120                 125             


Lys Ile Gln Tyr Gln Arg Glu Asn Pro Thr Glu Lys Gly Leu Asp Phe 
    130                 135                 140                 


Lys Leu Tyr Trp Thr Asp Ser Gln Asn Lys Lys Glu Val Ile Ser Ser 
145                 150                 155                 160 


Asp Asn Leu Gln Leu Pro Glu Leu Lys Gln Lys Ser Ser Asn Ser Arg 
                165                 170                 175     


Lys Lys Arg Ser Thr Ser Ala Gly Pro Thr Val Pro Asp Arg Asp Asn 
            180                 185                 190         


Asp Gly Ile Pro Asp Ser Leu Glu Val Glu Gly Tyr Thr Val Asp Val 
        195                 200                 205             


Lys Asn Lys Arg Thr Phe Leu Ser Pro Trp Ile Ser Asn Ile His Glu 
    210                 215                 220                 


Lys Lys Gly Leu Thr Lys Tyr Lys Ser Ser Pro Glu Lys Trp Ser Thr 
225                 230                 235                 240 


Ala Ser Asp Pro Tyr Ser Asp Phe Glu Lys Val Thr Gly Arg Ile Asp 
                245                 250                 255     


Lys Asn Val Ser Pro Glu Ala Arg His Pro Leu Val Ala Ala Tyr Pro 
            260                 265                 270         


Ile Val His Val Asp Met Glu Asn Ile Ile Leu Ser Lys Asn Glu Asp 
        275                 280                 285             


Gln Ser Thr Gln Asn Thr Asp Ser Gln Thr Arg Thr Ile Ser Lys Asn 
    290                 295                 300                 


Thr Ser Thr Ser Arg Thr His Thr Ser Glu Val His Gly Asn Ala Glu 
305                 310                 315                 320 


Val His Ala Ser Phe Phe Asp Ile Gly Gly Ser Val Ser Ala Gly Phe 
                325                 330                 335     


Ser Asn Ser Asn Ser Ser Thr Val Ala Ile Asp His Ser Leu Ser Leu 
            340                 345                 350         


Ala Gly Glu Arg Thr Trp Ala Glu Thr Met Gly Leu Asn Thr Ala Asp 
        355                 360                 365             


Thr Ala Arg Leu Asn Ala Asn Ile Arg Tyr Val Asn Thr Gly Thr Ala 
    370                 375                 380                 


Pro Ile Tyr Asn Val Leu Pro Thr Thr Ser Leu Val Leu Gly Lys Asn 
385                 390                 395                 400 


Gln Thr Leu Ala Thr Ile Lys Ala Lys Glu Asn Gln Leu Ser Gln Ile 
                405                 410                 415     


Leu Ala Pro Asn Asn Tyr Tyr Pro Ser Lys Asn Leu Ala Pro Ile Ala 
            420                 425                 430         


Leu Asn Ala Gln Asp Asp Phe Ser Ser Thr Pro Ile Thr Met Asn Tyr 
        435                 440                 445             


Asn Gln Phe Leu Glu Leu Glu Lys Thr Lys Gln Leu Arg Leu Asp Thr 
    450                 455                 460                 


Asp Gln Val Tyr Gly Asn Ile Ala Thr Tyr Asn Phe Glu Asn Gly Arg 
465                 470                 475                 480 


Val Arg Val Asp Thr Gly Ser Asn Trp Ser Glu Val Leu Pro Gln Ile 
                485                 490                 495     


Gln Glu Thr Thr Ala Arg Ile Ile Phe Asn Gly Lys Asp Leu Asn Leu 
            500                 505                 510         


Val Glu Arg Arg Ile Ala Ala Val Asn Pro Ser Lys Pro Leu Glu Thr 
        515                 520                 525             


Thr Lys Pro Asp Met Thr Leu Lys Glu Ala Leu Lys Ile Ala Phe Gly 
    530                 535                 540                 


Phe Asn Glu Pro Asn Gly Asn Leu Gln Tyr Gln Gly Lys Asp Ile Thr 
545                 550                 555                 560 


Glu Phe Asp Phe Asn Phe Asp Gln Gln Thr Ser Gln Asn Ile Lys Asn 
                565                 570                 575     


Gln Leu Ala Glu Leu Asn Ala Thr Asn Ile Tyr Thr Val Leu Asp Lys 
            580                 585                 590         


Ile Lys Leu Asn Ala Lys Met Asn Ile Leu Ile Arg Asp Lys Arg Phe 
        595                 600                 605             


His Tyr Asp Arg Asn Asn Ile Ala Val Gly Ala Asp Glu Ser Val Val 
    610                 615                 620                 


Lys Glu Ala His Arg Glu Val Ile Asn Ser Ser Thr Glu Gly Leu Leu 
625                 630                 635                 640 


Leu Asn Ile Asp Lys Asp Ile Arg Lys Ile Leu Ser Gly Tyr Ile Val 
                645                 650                 655     


Glu Ile Glu Asp Thr Glu Gly Leu Lys Glu Val Ile Asn Asp Arg Tyr 
            660                 665                 670         


Asp Met Leu Asn Ile Ser Ser Leu Arg Gln Asp Gly Lys Thr Phe Ile 
        675                 680                 685             


Asp Phe Lys Lys Tyr Asn Asp Lys Leu Pro Leu Tyr Ile Ser Asn Pro 
    690                 695                 700                 


Asn Tyr Lys Val Asn Val Tyr Ala Val Thr Lys Glu Asn Thr Ile Ile 
705                 710                 715                 720 


Asn Pro Ser Glu Asn Gly Asp Thr Ser Thr Asn Gly Ile Lys Lys Ile 
                725                 730                 735     


Leu Ile Phe Ser Lys Lys Gly Tyr Glu Ile Gly 
            740                 745         


<210>  8
<211>  747
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B. anthracis PA83 K245G; R252N [16] mutant

<400>  8

Met Arg Gly Ser His His His His His His Gly Ser Glu Val Lys Gln 
1               5                   10                  15      


Glu Asn Arg Leu Leu Asn Glu Ser Glu Ser Ser Ser Gln Gly Leu Leu 
            20                  25                  30          


Gly Tyr Tyr Phe Ser Asp Leu Asn Phe Gln Ala Pro Met Val Val Thr 
        35                  40                  45              


Ser Ser Thr Thr Gly Asp Leu Ser Ile Pro Ser Ser Glu Leu Glu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Asn Gln Tyr Phe Gln Ser Ala Ile Trp Ser Gly Phe 
65                  70                  75                  80  


Ile Lys Val Lys Lys Ser Asp Glu Tyr Thr Phe Ala Thr Ser Ala Asp 
                85                  90                  95      


Asn His Val Thr Met Trp Val Asp Asp Gln Glu Val Ile Asn Lys Ala 
            100                 105                 110         


Ser Asn Ser Asn Lys Ile Arg Leu Glu Lys Gly Arg Leu Tyr Gln Ile 
        115                 120                 125             


Lys Ile Gln Tyr Gln Arg Glu Asn Pro Thr Glu Lys Gly Leu Asp Phe 
    130                 135                 140                 


Lys Leu Tyr Trp Thr Asp Ser Gln Asn Lys Lys Glu Val Ile Ser Ser 
145                 150                 155                 160 


Asp Asn Leu Gln Leu Pro Glu Leu Lys Gln Lys Ser Ser Asn Ser Arg 
                165                 170                 175     


Lys Lys Arg Ser Thr Ser Ala Gly Pro Thr Val Pro Asp Arg Asp Asn 
            180                 185                 190         


Asp Gly Ile Pro Asp Ser Leu Glu Val Glu Gly Tyr Thr Val Asp Val 
        195                 200                 205             


Lys Asn Lys Arg Thr Phe Leu Ser Pro Trp Ile Ser Asn Ile His Glu 
    210                 215                 220                 


Lys Lys Gly Leu Thr Lys Tyr Lys Ser Ser Pro Glu Lys Trp Ser Thr 
225                 230                 235                 240 


Ala Ser Asp Pro Tyr Ser Asp Phe Glu Lys Val Thr Gly Arg Ile Asp 
                245                 250                 255     


Gly Asn Val Ser Pro Glu Ala Asn His Pro Leu Val Ala Ala Tyr Pro 
            260                 265                 270         


Ile Val His Val Asp Met Glu Asn Ile Ile Leu Ser Lys Asn Glu Asp 
        275                 280                 285             


Gln Ser Thr Gln Asn Thr Asp Ser Gln Thr Arg Thr Ile Ser Lys Asn 
    290                 295                 300                 


Thr Ser Thr Ser Arg Thr His Thr Ser Glu Val His Gly Asn Ala Glu 
305                 310                 315                 320 


Val His Ala Ser Phe Phe Asp Ile Gly Gly Ser Val Ser Ala Gly Phe 
                325                 330                 335     


Ser Asn Ser Asn Ser Ser Thr Val Ala Ile Asp His Ser Leu Ser Leu 
            340                 345                 350         


Ala Gly Glu Arg Thr Trp Ala Glu Thr Met Gly Leu Asn Thr Ala Asp 
        355                 360                 365             


Thr Ala Arg Leu Asn Ala Asn Ile Arg Tyr Val Asn Thr Gly Thr Ala 
    370                 375                 380                 


Pro Ile Tyr Asn Val Leu Pro Thr Thr Ser Leu Val Leu Gly Lys Asn 
385                 390                 395                 400 


Gln Thr Leu Ala Thr Ile Lys Ala Lys Glu Asn Gln Leu Ser Gln Ile 
                405                 410                 415     


Leu Ala Pro Asn Asn Tyr Tyr Pro Ser Lys Asn Leu Ala Pro Ile Ala 
            420                 425                 430         


Leu Asn Ala Gln Asp Asp Phe Ser Ser Thr Pro Ile Thr Met Asn Tyr 
        435                 440                 445             


Asn Gln Phe Leu Glu Leu Glu Lys Thr Lys Gln Leu Arg Leu Asp Thr 
    450                 455                 460                 


Asp Gln Val Tyr Gly Asn Ile Ala Thr Tyr Asn Phe Glu Asn Gly Arg 
465                 470                 475                 480 


Val Arg Val Asp Thr Gly Ser Asn Trp Ser Glu Val Leu Pro Gln Ile 
                485                 490                 495     


Gln Glu Thr Thr Ala Arg Ile Ile Phe Asn Gly Lys Asp Leu Asn Leu 
            500                 505                 510         


Val Glu Arg Arg Ile Ala Ala Val Asn Pro Ser Asp Pro Leu Glu Thr 
        515                 520                 525             


Thr Lys Pro Asp Met Thr Leu Lys Glu Ala Leu Lys Ile Ala Phe Gly 
    530                 535                 540                 


Phe Asn Glu Pro Asn Gly Asn Leu Gln Tyr Gln Gly Lys Asp Ile Thr 
545                 550                 555                 560 


Glu Phe Asp Phe Asn Phe Asp Gln Gln Thr Ser Gln Asn Ile Lys Asn 
                565                 570                 575     


Gln Leu Ala Glu Leu Asn Ala Thr Asn Ile Tyr Thr Val Leu Asp Lys 
            580                 585                 590         


Ile Lys Leu Asn Ala Lys Met Asn Ile Leu Ile Arg Asp Lys Arg Phe 
        595                 600                 605             


His Tyr Asp Arg Asn Asn Ile Ala Val Gly Ala Asp Glu Ser Val Val 
    610                 615                 620                 


Lys Glu Ala His Arg Glu Val Ile Asn Ser Ser Thr Glu Gly Leu Leu 
625                 630                 635                 640 


Leu Asn Ile Asp Lys Asp Ile Arg Lys Ile Leu Ser Gly Tyr Ile Val 
                645                 650                 655     


Glu Ile Glu Asp Thr Glu Gly Leu Lys Glu Val Ile Asn Asp Arg Tyr 
            660                 665                 670         


Asp Met Leu Asn Ile Ser Ser Leu Arg Gln Asp Gly Lys Thr Phe Ile 
        675                 680                 685             


Asp Phe Lys Lys Tyr Asn Asp Lys Leu Pro Leu Tyr Ile Ser Asn Pro 
    690                 695                 700                 


Asn Tyr Lys Val Asn Val Tyr Ala Val Thr Lys Glu Asn Thr Ile Ile 
705                 710                 715                 720 


Asn Pro Ser Glu Asn Gly Asp Thr Ser Thr Asn Gly Ile Lys Lys Ile 
                725                 730                 735     


Leu Ile Phe Ser Lys Lys Gly Tyr Glu Ile Gly 
            740                 745         


<210>  9
<211>  747
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B. anthracis PA83 K245N; R252S [16] mutant

<400>  9

Met Arg Gly Ser His His His His His His Gly Ser Glu Val Lys Gln 
1               5                   10                  15      


Glu Asn Arg Leu Leu Asn Glu Ser Glu Ser Ser Ser Gln Gly Leu Leu 
            20                  25                  30          


Gly Tyr Tyr Phe Ser Asp Leu Asn Phe Gln Ala Pro Met Val Val Thr 
        35                  40                  45              


Ser Ser Thr Thr Gly Asp Leu Ser Ile Pro Ser Ser Glu Leu Glu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Asn Gln Tyr Phe Gln Ser Ala Ile Trp Ser Gly Phe 
65                  70                  75                  80  


Ile Lys Val Lys Lys Ser Asp Glu Tyr Thr Phe Ala Thr Ser Ala Asp 
                85                  90                  95      


Asn His Val Thr Met Trp Val Asp Asp Gln Glu Val Ile Asn Lys Ala 
            100                 105                 110         


Ser Asn Ser Asn Lys Ile Arg Leu Glu Lys Gly Arg Leu Tyr Gln Ile 
        115                 120                 125             


Lys Ile Gln Tyr Gln Arg Glu Asn Pro Thr Glu Lys Gly Leu Asp Phe 
    130                 135                 140                 


Lys Leu Tyr Trp Thr Asp Ser Gln Asn Lys Lys Glu Val Ile Ser Ser 
145                 150                 155                 160 


Asp Asn Leu Gln Leu Pro Glu Leu Lys Gln Lys Ser Ser Asn Ser Arg 
                165                 170                 175     


Lys Lys Arg Ser Thr Ser Ala Gly Pro Thr Val Pro Asp Arg Asp Asn 
            180                 185                 190         


Asp Gly Ile Pro Asp Ser Leu Glu Val Glu Gly Tyr Thr Val Asp Val 
        195                 200                 205             


Lys Asn Lys Arg Thr Phe Leu Ser Pro Trp Ile Ser Asn Ile His Glu 
    210                 215                 220                 


Lys Lys Gly Leu Thr Lys Tyr Lys Ser Ser Pro Glu Lys Trp Ser Thr 
225                 230                 235                 240 


Ala Ser Asp Pro Tyr Ser Asp Phe Glu Lys Val Thr Gly Arg Ile Asp 
                245                 250                 255     


Asn Asn Val Ser Pro Glu Ala Ser His Pro Leu Val Ala Ala Tyr Pro 
            260                 265                 270         


Ile Val His Val Asp Met Glu Asn Ile Ile Leu Ser Lys Asn Glu Asp 
        275                 280                 285             


Gln Ser Thr Gln Asn Thr Asp Ser Gln Thr Arg Thr Ile Ser Lys Asn 
    290                 295                 300                 


Thr Ser Thr Ser Arg Thr His Thr Ser Glu Val His Gly Asn Ala Glu 
305                 310                 315                 320 


Val His Ala Ser Phe Phe Asp Ile Gly Gly Ser Val Ser Ala Gly Phe 
                325                 330                 335     


Ser Asn Ser Asn Ser Ser Thr Val Ala Ile Asp His Ser Leu Ser Leu 
            340                 345                 350         


Ala Gly Glu Arg Thr Trp Ala Glu Thr Met Gly Leu Asn Thr Ala Asp 
        355                 360                 365             


Thr Ala Arg Leu Asn Ala Asn Ile Arg Tyr Val Asn Thr Gly Thr Ala 
    370                 375                 380                 


Pro Ile Tyr Asn Val Leu Pro Thr Thr Ser Leu Val Leu Gly Lys Asn 
385                 390                 395                 400 


Gln Thr Leu Ala Thr Ile Lys Ala Lys Glu Asn Gln Leu Ser Gln Ile 
                405                 410                 415     


Leu Ala Pro Asn Asn Tyr Tyr Pro Ser Lys Asn Leu Ala Pro Ile Ala 
            420                 425                 430         


Leu Asn Ala Gln Asp Asp Phe Ser Ser Thr Pro Ile Thr Met Asn Tyr 
        435                 440                 445             


Asn Gln Phe Leu Glu Leu Glu Lys Thr Lys Gln Leu Arg Leu Asp Thr 
    450                 455                 460                 


Asp Gln Val Tyr Gly Asn Ile Ala Thr Tyr Asn Phe Glu Asn Gly Arg 
465                 470                 475                 480 


Val Arg Val Asp Thr Gly Ser Asn Trp Ser Glu Val Leu Pro Gln Ile 
                485                 490                 495     


Gln Glu Thr Thr Ala Arg Ile Ile Phe Asn Gly Lys Asp Leu Asn Leu 
            500                 505                 510         


Val Glu Arg Arg Ile Ala Ala Val Asn Pro Ser Asp Pro Leu Glu Thr 
        515                 520                 525             


Thr Lys Pro Asp Met Thr Leu Lys Glu Ala Leu Lys Ile Ala Phe Gly 
    530                 535                 540                 


Phe Asn Glu Pro Asn Gly Asn Leu Gln Tyr Gln Gly Lys Asp Ile Thr 
545                 550                 555                 560 


Glu Phe Asp Phe Asn Phe Asp Gln Gln Thr Ser Gln Asn Ile Lys Asn 
                565                 570                 575     


Gln Leu Ala Glu Leu Asn Ala Thr Asn Ile Tyr Thr Val Leu Asp Lys 
            580                 585                 590         


Ile Lys Leu Asn Ala Lys Met Asn Ile Leu Ile Arg Asp Lys Arg Phe 
        595                 600                 605             


His Tyr Asp Arg Asn Asn Ile Ala Val Gly Ala Asp Glu Ser Val Val 
    610                 615                 620                 


Lys Glu Ala His Arg Glu Val Ile Asn Ser Ser Thr Glu Gly Leu Leu 
625                 630                 635                 640 


Leu Asn Ile Asp Lys Asp Ile Arg Lys Ile Leu Ser Gly Tyr Ile Val 
                645                 650                 655     


Glu Ile Glu Asp Thr Glu Gly Leu Lys Glu Val Ile Asn Asp Arg Tyr 
            660                 665                 670         


Asp Met Leu Asn Ile Ser Ser Leu Arg Gln Asp Gly Lys Thr Phe Ile 
        675                 680                 685             


Asp Phe Lys Lys Tyr Asn Asp Lys Leu Pro Leu Tyr Ile Ser Asn Pro 
    690                 695                 700                 


Asn Tyr Lys Val Asn Val Tyr Ala Val Thr Lys Glu Asn Thr Ile Ile 
705                 710                 715                 720 


Asn Pro Ser Glu Asn Gly Asp Thr Ser Thr Asn Gly Ile Lys Lys Ile 
                725                 730                 735     


Leu Ile Phe Ser Lys Lys Gly Tyr Glu Ile Gly 
            740                 745         


<210>  10
<211>  738
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B. anthracis PA83-HL hybrid molecule

<400>  10

Gly Ser Glu Val Lys Gln Glu Asn Arg Leu Leu Asn Glu Ser Glu Ser 
1               5                   10                  15      


Ser Ser Gln Gly Leu Leu Gly Tyr Tyr Phe Ser Asp Leu Asn Phe Gln 
            20                  25                  30          


Ala Pro Met Val Val Thr Ser Ser Thr Thr Gly Asp Leu Ser Ile Pro 
        35                  40                  45              


Ser Ser Glu Leu Glu Asn Ile Pro Ser Glu Asn Gln Tyr Phe Gln Ser 
    50                  55                  60                  


Ala Ile Trp Ser Gly Phe Ile Lys Val Lys Lys Ser Asp Glu Tyr Thr 
65                  70                  75                  80  


Phe Ala Thr Ser Ala Asp Asn His Val Thr Met Trp Val Asp Asp Gln 
                85                  90                  95      


Glu Val Ile Asn Lys Ala Ser Asn Ser Asn Lys Ile Arg Leu Glu Lys 
            100                 105                 110         


Gly Arg Leu Tyr Gln Ile Lys Ile Gln Tyr Gln Arg Glu Asn Pro Thr 
        115                 120                 125             


Glu Lys Gly Leu Asp Phe Lys Leu Tyr Trp Thr Asp Ser Gln Asn Lys 
    130                 135                 140                 


Lys Glu Val Ile Ser Ser Asp Asn Leu Gln Leu Pro Glu Leu Lys Gln 
145                 150                 155                 160 


Lys Ser Ser Asn Ser Arg Lys Lys Arg Ser Thr Ser Ala Gly Pro Thr 
                165                 170                 175     


Val Pro Asp Arg Asp Asn Asp Gly Ile Pro Asp Ser Leu Glu Val Glu 
            180                 185                 190         


Gly Tyr Thr Val Asp Val Lys Asn Lys Arg Thr Phe Leu Ser Pro Trp 
        195                 200                 205             


Ile Ser Asn Ile His Glu Lys Lys Gly Leu Thr Lys Tyr Lys Ser Ser 
    210                 215                 220                 


Pro Glu Lys Trp Ser Thr Ala Ser Asp Pro Tyr Ser Asp Phe Glu Lys 
225                 230                 235                 240 


Val Thr Gly Arg Ile Asp Lys Asn Val Ser Pro Glu Ala Arg His Pro 
                245                 250                 255     


Leu Val Ala Ala Tyr Pro Ile Val His Val Asp Met Glu Asn Ile Ile 
            260                 265                 270         


Leu Ser Lys Asn Glu Asp Gln Ser Thr Gln Asn Thr Asp Ser Gln Thr 
        275                 280                 285             


Arg Thr Ile Ser Lys Asn Thr Ser Lys Glu Tyr Met Ser Thr Leu Thr 
    290                 295                 300                 


Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp Thr Gly Lys Ile Gly 
305                 310                 315                 320 


Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Ala 
                325                 330                 335     


Ile Asp His Ser Leu Ser Leu Ala Gly Glu Arg Thr Trp Ala Glu Thr 
            340                 345                 350         


Met Gly Leu Asn Thr Ala Asp Thr Ala Arg Leu Asn Ala Asn Ile Arg 
        355                 360                 365             


Tyr Val Asn Thr Gly Thr Ala Pro Ile Tyr Asn Val Leu Pro Thr Thr 
    370                 375                 380                 


Ser Leu Val Leu Gly Lys Asn Gln Thr Leu Ala Thr Ile Lys Ala Lys 
385                 390                 395                 400 


Glu Asn Gln Leu Ser Gln Ile Leu Ala Pro Asn Asn Tyr Tyr Pro Ser 
                405                 410                 415     


Lys Asn Leu Ala Pro Ile Ala Leu Asn Ala Gln Asp Asp Phe Ser Ser 
            420                 425                 430         


Thr Pro Ile Thr Met Asn Tyr Asn Gln Phe Leu Glu Leu Glu Lys Thr 
        435                 440                 445             


Lys Gln Leu Arg Leu Asp Thr Asp Gln Val Tyr Gly Asn Ile Ala Thr 
    450                 455                 460                 


Tyr Asn Phe Glu Asn Gly Arg Val Arg Val Asp Thr Gly Ser Asn Trp 
465                 470                 475                 480 


Ser Glu Val Leu Pro Gln Ile Gln Glu Thr Thr Ala Arg Ile Ile Phe 
                485                 490                 495     


Asn Gly Lys Asp Leu Asn Leu Val Glu Arg Arg Ile Ala Ala Val Asn 
            500                 505                 510         


Pro Ser Asp Pro Leu Glu Thr Thr Lys Pro Asp Met Thr Leu Lys Glu 
        515                 520                 525             


Ala Leu Lys Ile Ala Phe Gly Phe Asn Glu Pro Asn Gly Asn Leu Gln 
    530                 535                 540                 


Tyr Gln Gly Lys Asp Ile Thr Glu Phe Asp Phe Asn Phe Asp Gln Gln 
545                 550                 555                 560 


Thr Ser Gln Asn Ile Lys Asn Gln Leu Ala Glu Leu Asn Ala Thr Asn 
                565                 570                 575     


Ile Tyr Thr Val Leu Asp Lys Ile Lys Leu Asn Ala Lys Met Asn Ile 
            580                 585                 590         


Leu Ile Arg Asp Lys Arg Phe His Tyr Asp Arg Asn Asn Ile Ala Val 
        595                 600                 605             


Gly Ala Asp Glu Ser Val Val Lys Glu Ala His Arg Glu Val Ile Asn 
    610                 615                 620                 


Ser Ser Thr Glu Gly Leu Leu Leu Asn Ile Asp Lys Asp Ile Arg Lys 
625                 630                 635                 640 


Ile Leu Ser Gly Tyr Ile Val Glu Ile Glu Asp Thr Glu Gly Leu Lys 
                645                 650                 655     


Glu Val Ile Asn Asp Arg Tyr Asp Met Leu Asn Ile Ser Ser Leu Arg 
            660                 665                 670         


Gln Asp Gly Lys Thr Phe Ile Asp Phe Lys Lys Tyr Asn Asp Lys Leu 
        675                 680                 685             


Pro Leu Tyr Ile Ser Asn Pro Asn Tyr Lys Val Asn Val Tyr Ala Val 
    690                 695                 700                 


Thr Lys Glu Asn Thr Ile Ile Asn Pro Ser Glu Asn Gly Asp Thr Ser 
705                 710                 715                 720 


Thr Asn Gly Ile Lys Lys Ile Leu Ile Phe Ser Lys Lys Gly Tyr Glu 
                725                 730                 735     


Ile Gly 
        


<210>  11
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  lethal factor domain I (LFn)

<400>  11

Met Glu Arg Asn Lys Thr Gln Glu Glu His Leu Lys Glu Ile Met Lys 
1               5                   10                  15      


His Ile Val Lys Ile Glu Val Lys Gly Glu Glu Ala Val Lys Lys Glu 
            20                  25                  30          


Ala Ala Glu Lys Leu Leu Glu Lys Val Pro Ser Asp Val Leu Glu Met 
        35                  40                  45              


Tyr Lys Ala Ile Gly Gly Lys Ile Tyr Ile Val Asp Gly Asp Ile Thr 
    50                  55                  60                  


Lys His Ile Ser Leu Glu Ala Leu Ser Glu Asp Lys Lys Lys Ile Lys 
65                  70                  75                  80  


Asp Ile Tyr Gly Lys Asp Ala Leu Leu His Glu His Tyr Val Tyr Ala 
                85                  90                  95      


Lys Glu Gly Tyr Glu Pro Val Leu Val Ile Gln Ser Ser Glu Asp Tyr 
            100                 105                 110         


Val Glu Asn Thr Glu Lys Ala Leu Asn Val Tyr Tyr Glu Ile Gly Lys 
        115                 120                 125             


Ile Leu Ser Arg Asp Ile Leu Ser Lys Ile Asn Gln Pro Tyr Gln Lys 
    130                 135                 140                 


Phe Leu Asp Val Leu Asn Thr Ile Lys Asn Ala Ser Asp Ser Asp Gly 
145                 150                 155                 160 


Gln Asp Leu Leu Phe Thr Asn Gln Leu Lys Glu His Pro Thr Asp Phe 
                165                 170                 175     


Ser Val Glu Phe Leu Glu Gln Asn Ser Asn Glu Val Gln Glu Val Phe 
            180                 185                 190         


Ala Lys Ala Phe Ala Tyr Tyr Ile Glu Pro Gln His Arg Asp Val Leu 
        195                 200                 205             


Gln Leu Tyr Ala Pro Glu Ala Phe Asn Tyr Met Asp Lys Phe Asn Glu 
    210                 215                 220                 


Gln Glu Ile Asn Leu Ser 
225                 230 


<210>  12
<211>  398
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Saccharomyces cerevisiae GAL4 (fused with LFn)

<400>  12

Met Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Met 
1               5                   10                  15      


Glu Arg Asn Lys Thr Gln Glu Glu His Leu Lys Glu Ile Met Lys His 
            20                  25                  30          


Ile Val Lys Ile Glu Val Lys Gly Glu Glu Ala Val Lys Lys Glu Ala 
        35                  40                  45              


Ala Glu Lys Leu Leu Glu Lys Val Pro Ser Asp Val Leu Glu Met Tyr 
    50                  55                  60                  


Lys Ala Ile Gly Gly Lys Ile Tyr Ile Val Asp Gly Asp Ile Thr Lys 
65                  70                  75                  80  


His Ile Ser Leu Glu Ala Leu Ser Glu Asp Lys Lys Lys Ile Lys Asp 
                85                  90                  95      


Ile Tyr Gly Lys Asp Ala Leu Leu His Glu His Tyr Val Tyr Ala Lys 
            100                 105                 110         


Glu Gly Tyr Glu Pro Val Leu Val Ile Gln Ser Ser Glu Asp Tyr Val 
        115                 120                 125             


Glu Asn Thr Glu Lys Ala Leu Asn Val Tyr Tyr Glu Ile Gly Lys Ile 
    130                 135                 140                 


Leu Ser Arg Asp Ile Leu Ser Lys Ile Asn Gln Pro Tyr Gln Lys Phe 
145                 150                 155                 160 


Leu Asp Val Leu Asn Thr Ile Lys Asn Ala Ser Asp Ser Asp Gly Gln 
                165                 170                 175     


Asp Leu Leu Phe Thr Asn Gln Leu Lys Glu His Pro Thr Asp Phe Ser 
            180                 185                 190         


Val Glu Phe Leu Glu Gln Asn Ser Asn Glu Val Gln Glu Val Phe Ala 
        195                 200                 205             


Lys Ala Phe Ala Tyr Tyr Ile Glu Pro Gln His Arg Asp Val Leu Gln 
    210                 215                 220                 


Leu Tyr Ala Pro Glu Ala Phe Asn Tyr Met Asp Lys Phe Asn Glu Gln 
225                 230                 235                 240 


Glu Ile Asn Leu Ser Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys 
                245                 250                 255     


Asp Ile Cys Arg Leu Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys 
            260                 265                 270         


Cys Ala Lys Cys Leu Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys 
        275                 280                 285             


Thr Lys Arg Ser Pro Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser 
    290                 295                 300                 


Arg Leu Glu Arg Leu Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu 
305                 310                 315                 320 


Asp Leu Asp Met Ile Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala 
                325                 330                 335     


Leu Leu Thr Gly Leu Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val 
            340                 345                 350         


Thr Asp Arg Leu Ala Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg 
        355                 360                 365             


Gln His Arg Ile Ser Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys 
    370                 375                 380                 


Gly Gln Arg Gln Leu Thr Val Ser His His His His His His 
385                 390                 395             


<210>  13
<211>  329
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein Kinase R

<400>  13

Met Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Met 
1               5                   10                  15      


Glu Arg Asn Lys Thr Gln Glu Glu His Leu Lys Glu Ile Met Lys His 
            20                  25                  30          


Ile Val Lys Ile Glu Val Lys Gly Glu Glu Ala Val Lys Lys Glu Ala 
        35                  40                  45              


Ala Glu Lys Leu Leu Glu Lys Val Pro Ser Asp Val Leu Glu Met Tyr 
    50                  55                  60                  


Lys Ala Ile Gly Gly Lys Ile Tyr Ile Val Asp Gly Asp Ile Thr Lys 
65                  70                  75                  80  


His Ile Ser Leu Glu Ala Leu Ser Glu Asp Lys Lys Lys Ile Lys Asp 
                85                  90                  95      


Ile Tyr Gly Lys Asp Ala Leu Leu His Glu His Tyr Val Tyr Ala Lys 
            100                 105                 110         


Glu Gly Tyr Glu Pro Val Leu Val Ile Gln Ser Ser Glu Asp Tyr Val 
        115                 120                 125             


Glu Asn Thr Glu Lys Ala Leu Asn Val Tyr Tyr Glu Ile Gly Lys Ile 
    130                 135                 140                 


Leu Ser Arg Asp Ile Leu Ser Lys Ile Asn Gln Pro Tyr Gln Lys Phe 
145                 150                 155                 160 


Leu Asp Val Leu Asn Thr Ile Lys Asn Ala Ser Asp Ser Asp Gly Gln 
                165                 170                 175     


Asp Leu Leu Phe Thr Asn Gln Leu Lys Glu His Pro Thr Asp Phe Ser 
            180                 185                 190         


Val Glu Phe Leu Glu Gln Asn Ser Asn Glu Val Gln Glu Val Phe Ala 
        195                 200                 205             


Lys Ala Phe Ala Tyr Tyr Ile Glu Pro Gln His Arg Asp Val Leu Gln 
    210                 215                 220                 


Leu Tyr Ala Pro Glu Ala Phe Asn Tyr Met Asp Lys Phe Asn Glu Gln 
225                 230                 235                 240 


Glu Ile Asn Leu Ser Met Ala Gly Asp Leu Ser Ala Gly Phe Phe Met 
                245                 250                 255     


Glu Glu Leu Asn Thr Tyr Arg Gln Lys Gln Gly Val Val Leu Lys Tyr 
            260                 265                 270         


Gln Glu Leu Pro Asn Ser Gly Pro Pro His Asp Arg Arg Phe Thr Phe 
        275                 280                 285             


Gln Val Ile Ile Asp Gly Arg Glu Phe Pro Glu Gly Glu Gly Arg Ser 
    290                 295                 300                 


Lys Lys Glu Ala Lys Asn Ala Ala Ala Lys Leu Ala Val Glu Ile Leu 
305                 310                 315                 320 


Asn Lys Glu His His His His His His 
                325                 


<210>  14
<211>  1328
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 coupled to LFn

<400>  14

Met Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Met 
1               5                   10                  15      


Glu Arg Asn Lys Thr Gln Glu Glu His Leu Lys Glu Ile Met Lys His 
            20                  25                  30          


Ile Val Lys Ile Glu Val Lys Gly Glu Glu Ala Val Lys Lys Glu Ala 
        35                  40                  45              


Ala Glu Lys Leu Leu Glu Lys Val Pro Ser Asp Val Leu Glu Met Tyr 
    50                  55                  60                  


Lys Ala Ile Gly Gly Lys Ile Tyr Ile Val Asp Gly Asp Ile Thr Lys 
65                  70                  75                  80  


His Ile Ser Leu Glu Ala Leu Ser Glu Asp Lys Lys Lys Ile Lys Asp 
                85                  90                  95      


Ile Tyr Gly Lys Asp Ala Leu Leu His Glu His Tyr Val Tyr Ala Lys 
            100                 105                 110         


Glu Gly Tyr Glu Pro Val Leu Val Ile Gln Ser Ser Glu Asp Tyr Val 
        115                 120                 125             


Glu Asn Thr Glu Lys Ala Leu Asn Val Tyr Tyr Glu Ile Gly Lys Ile 
    130                 135                 140                 


Leu Ser Arg Asp Ile Leu Ser Lys Ile Asn Gln Pro Tyr Gln Lys Phe 
145                 150                 155                 160 


Leu Asp Val Leu Asn Thr Ile Lys Asn Ala Ser Asp Ser Asp Gly Gln 
                165                 170                 175     


Asp Leu Leu Phe Thr Asn Gln Leu Lys Glu His Pro Thr Asp Phe Ser 
            180                 185                 190         


Val Glu Phe Leu Glu Gln Asn Ser Asn Glu Val Gln Glu Val Phe Ala 
        195                 200                 205             


Lys Ala Phe Ala Tyr Tyr Ile Glu Pro Gln His Arg Asp Val Leu Gln 
    210                 215                 220                 


Leu Tyr Ala Pro Glu Ala Phe Asn Tyr Met Asp Lys Phe Asn Glu Gln 
225                 230                 235                 240 


Glu Ile Asn Leu Ser Leu Glu Val Leu Phe Gln Gly Pro Met Lys Arg 
                245                 250                 255     


Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Tyr Gly 
            260                 265                 270         


Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly Val Arg Leu 
        275                 280                 285             


Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg Ser Lys Arg 
    290                 295                 300                 


Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile Gln Arg Val 
305                 310                 315                 320 


Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His Ser Glu Leu 
                325                 330                 335     


Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu Ser Gln Lys 
            340                 345                 350         


Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu Ala Lys Arg 
        355                 360                 365             


Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr Gly Asn Glu 
    370                 375                 380                 


Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala Leu Glu Glu 
385                 390                 395                 400 


Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys Asp Gly Glu 
                405                 410                 415     


Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr Val Lys Glu 
            420                 425                 430         


Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln Leu Asp Gln 
        435                 440                 445             


Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg Arg Thr Tyr 
    450                 455                 460                 


Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys Asp Ile Lys 
465                 470                 475                 480 


Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe Pro Glu Glu 
                485                 490                 495     


Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr Asn Ala Leu 
            500                 505                 510         


Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn Glu Lys Leu 
        515                 520                 525             


Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe Lys Gln Lys 
    530                 535                 540                 


Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu Val Asn Glu 
545                 550                 555                 560 


Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys Pro Glu Phe 
                565                 570                 575     


Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr Ala Arg Lys 
            580                 585                 590         


Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala Lys Ile Leu 
        595                 600                 605             


Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu Thr Asn Leu 
    610                 615                 620                 


Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser Asn Leu Lys 
625                 630                 635                 640 


Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile Asn Leu Ile 
                645                 650                 655     


Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala Ile Phe Asn 
            660                 665                 670         


Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln Gln Lys Glu 
        675                 680                 685             


Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro Val Val Lys 
    690                 695                 700                 


Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile Ile Lys Lys 
705                 710                 715                 720 


Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg Glu Lys Asn 
                725                 730                 735     


Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys Arg Asn Arg 
            740                 745                 750         


Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr Gly Lys Glu 
        755                 760                 765             


Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp Met Gln Glu 
    770                 775                 780                 


Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu Asp Leu Leu 
785                 790                 795                 800 


Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro Arg Ser Val 
                805                 810                 815     


Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys Gln Glu Glu 
            820                 825                 830         


Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu Ser Ser Ser 
        835                 840                 845             


Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile Leu Asn Leu 
    850                 855                 860                 


Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu Tyr Leu Leu 
865                 870                 875                 880 


Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp Phe Ile Asn 
                885                 890                 895     


Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu Met Asn Leu 
            900                 905                 910         


Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys Val Lys Ser 
        915                 920                 925             


Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp Lys Phe Lys 
    930                 935                 940                 


Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp Ala Leu Ile 
945                 950                 955                 960 


Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys Leu Asp Lys 
                965                 970                 975     


Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys Gln Ala Glu 
            980                 985                 990         


Ser Met Pro Glu Ile Glu Thr Glu  Gln Glu Tyr Lys Glu  Ile Phe Ile 
        995                 1000                 1005             


Thr Pro  His Gln Ile Lys His  Ile Lys Asp Phe Lys  Asp Tyr Lys 
    1010                 1015                 1020             


Tyr Ser  His Arg Val Asp Lys  Lys Pro Asn Arg Glu  Leu Ile Asn 
    1025                 1030                 1035             


Asp Thr  Leu Tyr Ser Thr Arg  Lys Asp Asp Lys Gly  Asn Thr Leu 
    1040                 1045                 1050             


Ile Val  Asn Asn Leu Asn Gly  Leu Tyr Asp Lys Asp  Asn Asp Lys 
    1055                 1060                 1065             


Leu Lys  Lys Leu Ile Asn Lys  Ser Pro Glu Lys Leu  Leu Met Tyr 
    1070                 1075                 1080             


His His  Asp Pro Gln Thr Tyr  Gln Lys Leu Lys Leu  Ile Met Glu 
    1085                 1090                 1095             


Gln Tyr  Gly Asp Glu Lys Asn  Pro Leu Tyr Lys Tyr  Tyr Glu Glu 
    1100                 1105                 1110             


Thr Gly  Asn Tyr Leu Thr Lys  Tyr Ser Lys Lys Asp  Asn Gly Pro 
    1115                 1120                 1125             


Val Ile  Lys Lys Ile Lys Tyr  Tyr Gly Asn Lys Leu  Asn Ala His 
    1130                 1135                 1140             


Leu Asp  Ile Thr Asp Asp Tyr  Pro Asn Ser Arg Asn  Lys Val Val 
    1145                 1150                 1155             


Lys Leu  Ser Leu Lys Pro Tyr  Arg Phe Asp Val Tyr  Leu Asp Asn 
    1160                 1165                 1170             


Gly Val  Tyr Lys Phe Val Thr  Val Lys Asn Leu Asp  Val Ile Lys 
    1175                 1180                 1185             


Lys Glu  Asn Tyr Tyr Glu Val  Asn Ser Lys Cys Tyr  Glu Glu Ala 
    1190                 1195                 1200             


Lys Lys  Leu Lys Lys Ile Ser  Asn Gln Ala Glu Phe  Ile Ala Ser 
    1205                 1210                 1215             


Phe Tyr  Asn Asn Asp Leu Ile  Lys Ile Asn Gly Glu  Leu Tyr Arg 
    1220                 1225                 1230             


Val Ile  Gly Val Asn Asn Asp  Leu Leu Asn Arg Ile  Glu Val Asn 
    1235                 1240                 1245             


Met Ile  Asp Ile Thr Tyr Arg  Glu Tyr Leu Glu Asn  Met Asn Asp 
    1250                 1255                 1260             


Lys Arg  Pro Pro Arg Ile Ile  Lys Thr Ile Ala Ser  Lys Thr Gln 
    1265                 1270                 1275             


Ser Ile  Lys Lys Tyr Ser Thr  Asp Ile Leu Gly Asn  Leu Tyr Glu 
    1280                 1285                 1290             


Val Lys  Ser Lys Lys His Pro  Gln Ile Ile Lys Lys  Gly Tyr Pro 
    1295                 1300                 1305             


Tyr Asp  Val Pro Asp Tyr Ala  Glu Asn Leu Tyr Phe  Gln Gly His 
    1310                 1315                 1320             


His His  His His His 
    1325             


<210>  15
<211>  484
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Diphtheria toxin A (DTA) coupled to LFn

<400>  15

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ala Gly Gly His Gly Asp Val Gly Met His Val 
            20                  25                  30          


Lys Glu Lys Glu Lys Asn Lys Asp Glu Asn Lys Arg Lys Asp Glu Glu 
        35                  40                  45              


Arg Asn Lys Thr Gln Glu Glu His Leu Lys Glu Ile Met Lys His Ile 
    50                  55                  60                  


Val Lys Ile Glu Val Lys Gly Glu Glu Ala Val Lys Lys Glu Ala Ala 
65                  70                  75                  80  


Glu Lys Leu Leu Glu Lys Val Pro Ser Asp Val Leu Glu Met Tyr Lys 
                85                  90                  95      


Ala Ile Gly Gly Lys Ile Tyr Ile Val Asp Gly Asp Ile Thr Lys His 
            100                 105                 110         


Ile Ser Leu Glu Ala Leu Ser Glu Asp Lys Lys Lys Ile Lys Asp Ile 
        115                 120                 125             


Tyr Gly Lys Asp Ala Leu Leu His Glu His Tyr Val Tyr Ala Lys Glu 
    130                 135                 140                 


Gly Tyr Glu Pro Val Leu Val Ile Gln Ser Ser Glu Asp Tyr Val Glu 
145                 150                 155                 160 


Asn Thr Glu Lys Ala Leu Asn Val Tyr Tyr Glu Ile Gly Lys Ile Leu 
                165                 170                 175     


Ser Arg Asp Ile Leu Ser Lys Ile Asn Gln Pro Tyr Gln Lys Phe Leu 
            180                 185                 190         


Asp Val Leu Asn Thr Ile Lys Asn Ala Ser Asp Ser Asp Gly Gln Asp 
        195                 200                 205             


Leu Leu Phe Thr Asn Gln Leu Lys Glu His Pro Thr Asp Phe Ser Val 
    210                 215                 220                 


Glu Phe Leu Glu Gln Asn Ser Asn Glu Val Gln Glu Val Phe Ala Lys 
225                 230                 235                 240 


Ala Phe Ala Tyr Tyr Ile Glu Pro Gln His Arg Asp Val Leu Gln Leu 
                245                 250                 255     


Tyr Ala Pro Glu Ala Phe Asn Tyr Met Asp Lys Phe Asn Glu Gln Glu 
            260                 265                 270         


Ile Asn Leu Ser Ala Met Gly Ser Ser His His His His His His Ser 
        275                 280                 285             


Ser Gly Leu Val Pro Arg Gly Ala Asp Asp Val Val Asp Ser Ser Lys 
    290                 295                 300                 


Ser Phe Val Met Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly 
305                 310                 315                 320 


Tyr Val Asp Ser Ile Gln Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr 
                325                 330                 335     


Gln Gly Asn Tyr Asp Asp Asp Trp Lys Gly Phe Tyr Ser Thr Asp Asn 
            340                 345                 350         


Lys Tyr Asp Ala Ala Gly Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser 
        355                 360                 365             


Gly Lys Ala Gly Gly Val Val Lys Val Thr Tyr Pro Gly Leu Thr Lys 
    370                 375                 380                 


Val Leu Ala Leu Lys Val Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu 
385                 390                 395                 400 


Gly Leu Ser Leu Thr Glu Pro Leu Met Glu Gln Val Gly Thr Glu Glu 
                405                 410                 415     


Phe Ile Lys Arg Phe Gly Asp Gly Ala Ser Arg Val Val Leu Ser Leu 
            420                 425                 430         


Pro Phe Ala Glu Gly Ser Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu 
        435                 440                 445             


Gln Ala Lys Ala Leu Ser Val Glu Leu Glu Ile Asn Phe Glu Thr Arg 
    450                 455                 460                 


Gly Lys Arg Gly Gln Asp Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys 
465                 470                 475                 480 


Ala Gly Asn Arg 
                


<210>  16
<211>  1455
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  payload molecule

<400>  16
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atggcgggcg gtcatggtga tgtaggtatg cacgtaaaag agaaagagaa aaataaagat      120

gagaataaga gaaaagatga agaacgaaat aaaacacagg aagagcattt aaaggaaatc      180

atgaaacaca ttgtaaaaat agaagtaaaa ggggaggaag ctgttaaaaa agaggcagca      240

gaaaagctac ttgagaaagt accatctgat gttttagaga tgtataaagc aattggagga      300

aagatatata ttgtggatgg tgatattaca aaacatatat ctttagaagc attatctgaa      360

gataagaaaa aaataaaaga catttatggg aaagatgctt tattacatga acattatgta      420

tatgcaaaag aaggatatga acccgtactt gtaatccaat cttcggaaga ttatgtagaa      480

aatactgaaa aggcactgaa cgtttattat gaaataggta agatattatc aagggatatt      540

ttaagtaaaa ttaatcaacc atatcagaaa tttttagatg tattaaatac cattaaaaat      600

gcatctgatt cagatggaca agatctttta tttactaatc agcttaagga acatcccaca      660

gacttttctg tagaattctt ggaacaaaat agcaatgagg tacaagaagt atttgcgaaa      720

gcttttgcat attatatcga gccacagcat cgtgatgttt tacagcttta tgcaccggaa      780

gcttttaatt acatggataa atttaacgaa caagaaataa atctatccgc catgggcagc      840

tctcaccacc accaccacca ctcttccggc ctggttccac gtggtgctga cgacgttgtt      900

gactcttcta aatctttcgt tatggaaaac ttctcttctt accacggtac caaaccgggt      960

tacgtcgact ctatccagaa aggtatccag aagccgaaat ctggtaccca gggtaactac     1020

gacgacgact ggaaaggttt ctactctacc gacaacaaat acgacgccgc gggttactct     1080

gttgacaacg aaaacccgct gtctggtaaa gctggtggtg ttgttaaagt tacctacccg     1140

ggtctgacca aagttctggc tctgaaagtt gacaacgctg aaaccatcaa aaaagaactg     1200

ggtctctctc tgaccgaacc gctgatggaa caggttggta ccgaagaatt catcaaacgt     1260

ttcggtgacg gtgcttctcg tgttgttctg tctctgccgt tcgctgaggg ctcttcttct     1320

gttgaataca tcaacaactg ggaacaggct aaagctctgt ctgttgaact ggaaatcaac     1380

ttcgaaaccc gtggtaaacg tggccaggac gctatgtacg aatacatggc tcaggcttgt     1440

gcaggtaacc gttaa                                                      1455


<210>  17
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-Tandem dimeric tomato ASO sequence


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (2)..(2)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (3)..(3)
<223>  Guanine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (4)..(4)
<223>  Cytosine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (5)..(5)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (6)..(6)
<223>  Guanine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  Cytosine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  Cytosine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (10)..(10)
<223>  Adenine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (11)..(11)
<223>  Cytosine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (12)..(12)
<223>  Guanine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (13)..(13)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (14)..(14)
<223>  Adenine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (15)..(15)
<223>  Guanine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (16)..(16)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (17)..(17)
<223>  Adenine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (18)..(18)
<223>  Guanine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (19)..(19)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (20)..(20)
<223>  Adenine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (21)..(21)
<223>  Guanine with 3' Phosphorothioate modification

<400>  17
nnnnnnnnnn nnnnnnnnnn ngcatgccgg catcagagca gccggcat                    48


<210>  18
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-Tandem dimeric tomato ASO sequence


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (2)..(2)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (3)..(3)
<223>  Guanine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (4)..(4)
<223>  Cytosine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (5)..(5)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (6)..(6)
<223>  Guanine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  Cytosine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  Cytosine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (10)..(10)
<223>  Adenine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (11)..(11)
<223>  Cytosine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (12)..(12)
<223>  Guanine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (13)..(13)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (14)..(14)
<223>  Adenine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (15)..(15)
<223>  Guanine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (16)..(16)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (17)..(17)
<223>  Adenine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (18)..(18)
<223>  Guanine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (19)..(19)
<223>  Thymine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (20)..(20)
<223>  Adenine with 3' Phosphorothioate modification

<220>
<221>  modified_base
<222>  (21)..(21)
<223>  Guanine with 3' Phosphorothioate modification

<400>  18
nnnnnnnnnn nnnnnnnnnn ngcatgccgg ctgctctgat gccggcat                    48


