                         SEQUENCE LISTING

<110>  DUKE UNIVERSITY
 
<120>  PROTEIN C-FACTOR VII CHIMERAS

<130>  19-1529-WO

<150>  US 62/714,189
<151>  2018-08-03

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  466
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Val Ser Gln Ala Leu Arg Leu Leu Cys Leu Leu Leu Gly Leu Gln 
1               5                   10                  15      


Gly Cys Leu Ala Ala Gly Gly Val Ala Lys Ala Ser Gly Gly Glu Thr 
            20                  25                  30          


Arg Asp Met Pro Trp Lys Pro Gly Pro His Arg Val Phe Val Thr Gln 
        35                  40                  45              


Glu Glu Ala His Gly Val Leu His Arg Arg Arg Arg Ala Asn Ala Phe 
    50                  55                  60                  


Leu Glu Glu Leu Arg Pro Gly Ser Leu Glu Arg Glu Cys Lys Glu Glu 
65                  70                  75                  80  


Gln Cys Ser Phe Glu Glu Ala Arg Glu Ile Phe Lys Asp Ala Glu Arg 
                85                  90                  95      


Thr Lys Leu Phe Trp Ile Ser Tyr Ser Asp Gly Asp Gln Cys Ala Ser 
            100                 105                 110         


Ser Pro Cys Gln Asn Gly Gly Ser Cys Lys Asp Gln Leu Gln Ser Tyr 
        115                 120                 125             


Ile Cys Phe Cys Leu Pro Ala Phe Glu Gly Arg Asn Cys Glu Thr His 
    130                 135                 140                 


Lys Asp Asp Gln Leu Ile Cys Val Asn Glu Asn Gly Gly Cys Glu Gln 
145                 150                 155                 160 


Tyr Cys Ser Asp His Thr Gly Thr Lys Arg Ser Cys Arg Cys His Glu 
                165                 170                 175     


Gly Tyr Ser Leu Leu Ala Asp Gly Val Ser Cys Thr Pro Thr Val Glu 
            180                 185                 190         


Tyr Pro Cys Gly Lys Ile Pro Ile Leu Glu Lys Arg Asn Ala Ser Lys 
        195                 200                 205             


Pro Gln Gly Arg Ile Val Gly Gly Lys Val Cys Pro Lys Gly Glu Cys 
    210                 215                 220                 


Pro Trp Gln Val Leu Leu Leu Val Asn Gly Ala Gln Leu Cys Gly Gly 
225                 230                 235                 240 


Thr Leu Ile Asn Thr Ile Trp Val Val Ser Ala Ala His Cys Phe Asp 
                245                 250                 255     


Lys Ile Lys Asn Trp Arg Asn Leu Ile Ala Val Leu Gly Glu His Asp 
            260                 265                 270         


Leu Ser Glu His Asp Gly Asp Glu Gln Ser Arg Arg Val Ala Gln Val 
        275                 280                 285             


Ile Ile Pro Ser Thr Tyr Val Pro Gly Thr Thr Asn His Asp Ile Ala 
    290                 295                 300                 


Leu Leu Arg Leu His Gln Pro Val Val Leu Thr Asp His Val Val Pro 
305                 310                 315                 320 


Leu Cys Leu Pro Glu Arg Thr Phe Ser Glu Arg Thr Leu Ala Phe Val 
                325                 330                 335     


Arg Phe Ser Leu Val Ser Gly Trp Gly Gln Leu Leu Asp Arg Gly Ala 
            340                 345                 350         


Thr Ala Leu Glu Leu Met Val Leu Asn Val Pro Arg Leu Met Thr Gln 
        355                 360                 365             


Asp Cys Leu Gln Gln Ser Arg Lys Val Gly Asp Ser Pro Asn Ile Thr 
    370                 375                 380                 


Glu Tyr Met Phe Cys Ala Gly Tyr Ser Asp Gly Ser Lys Asp Ser Cys 
385                 390                 395                 400 


Lys Gly Asp Ser Gly Gly Pro His Ala Thr His Tyr Arg Gly Thr Trp 
                405                 410                 415     


Tyr Leu Thr Gly Ile Val Ser Trp Gly Gln Gly Cys Ala Thr Val Gly 
            420                 425                 430         


His Phe Gly Val Tyr Thr Arg Val Ser Gln Tyr Ile Glu Trp Leu Gln 
        435                 440                 445             


Lys Leu Met Arg Ser Glu Pro Arg Pro Gly Val Leu Leu Arg Ala Pro 
    450                 455                 460                 


Phe Pro 
465     


<210>  2
<211>  406
<212>  PRT
<213>  Homo sapiens

<400>  2

Ala Asn Ala Phe Leu Glu Glu Leu Arg Pro Gly Ser Leu Glu Arg Glu 
1               5                   10                  15      


Cys Lys Glu Glu Gln Cys Ser Phe Glu Glu Ala Arg Glu Ile Phe Lys 
            20                  25                  30          


Asp Ala Glu Arg Thr Lys Leu Phe Trp Ile Ser Tyr Ser Asp Gly Asp 
        35                  40                  45              


Gln Cys Ala Ser Ser Pro Cys Gln Asn Gly Gly Ser Cys Lys Asp Gln 
    50                  55                  60                  


Leu Gln Ser Tyr Ile Cys Phe Cys Leu Pro Ala Phe Glu Gly Arg Asn 
65                  70                  75                  80  


Cys Glu Thr His Lys Asp Asp Gln Leu Ile Cys Val Asn Glu Asn Gly 
                85                  90                  95      


Gly Cys Glu Gln Tyr Cys Ser Asp His Thr Gly Thr Lys Arg Ser Cys 
            100                 105                 110         


Arg Cys His Glu Gly Tyr Ser Leu Leu Ala Asp Gly Val Ser Cys Thr 
        115                 120                 125             


Pro Thr Val Glu Tyr Pro Cys Gly Lys Ile Pro Ile Leu Glu Lys Arg 
    130                 135                 140                 


Asn Ala Ser Lys Pro Gln Gly Arg Ile Val Gly Gly Lys Val Cys Pro 
145                 150                 155                 160 


Lys Gly Glu Cys Pro Trp Gln Val Leu Leu Leu Val Asn Gly Ala Gln 
                165                 170                 175     


Leu Cys Gly Gly Thr Leu Ile Asn Thr Ile Trp Val Val Ser Ala Ala 
            180                 185                 190         


His Cys Phe Asp Lys Ile Lys Asn Trp Arg Asn Leu Ile Ala Val Leu 
        195                 200                 205             


Gly Glu His Asp Leu Ser Glu His Asp Gly Asp Glu Gln Ser Arg Arg 
    210                 215                 220                 


Val Ala Gln Val Ile Ile Pro Ser Thr Tyr Val Pro Gly Thr Thr Asn 
225                 230                 235                 240 


His Asp Ile Ala Leu Leu Arg Leu His Gln Pro Val Val Leu Thr Asp 
                245                 250                 255     


His Val Val Pro Leu Cys Leu Pro Glu Arg Thr Phe Ser Glu Arg Thr 
            260                 265                 270         


Leu Ala Phe Val Arg Phe Ser Leu Val Ser Gly Trp Gly Gln Leu Leu 
        275                 280                 285             


Asp Arg Gly Ala Thr Ala Leu Glu Leu Met Val Leu Asn Val Pro Arg 
    290                 295                 300                 


Leu Met Thr Gln Asp Cys Leu Gln Gln Ser Arg Lys Val Gly Asp Ser 
305                 310                 315                 320 


Pro Asn Ile Thr Glu Tyr Met Phe Cys Ala Gly Tyr Ser Asp Gly Ser 
                325                 330                 335     


Lys Asp Ser Cys Lys Gly Asp Ser Gly Gly Pro His Ala Thr His Tyr 
            340                 345                 350         


Arg Gly Thr Trp Tyr Leu Thr Gly Ile Val Ser Trp Gly Gln Gly Cys 
        355                 360                 365             


Ala Thr Val Gly His Phe Gly Val Tyr Thr Arg Val Ser Gln Tyr Ile 
    370                 375                 380                 


Glu Trp Leu Gln Lys Leu Met Arg Ser Glu Pro Arg Pro Gly Val Leu 
385                 390                 395                 400 


Leu Arg Ala Pro Phe Pro 
                405     


<210>  3
<211>  461
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Trp Gln Leu Thr Ser Leu Leu Leu Phe Val Ala Thr Trp Gly Ile 
1               5                   10                  15      


Ser Gly Thr Pro Ala Pro Leu Asp Ser Val Phe Ser Ser Ser Glu Arg 
            20                  25                  30          


Ala His Gln Val Leu Arg Ile Arg Lys Arg Ala Asn Ser Phe Leu Glu 
        35                  40                  45              


Glu Leu Arg His Ser Ser Leu Glu Arg Glu Cys Ile Glu Glu Ile Cys 
    50                  55                  60                  


Asp Phe Glu Glu Ala Lys Glu Ile Phe Gln Asn Val Asp Asp Thr Leu 
65                  70                  75                  80  


Ala Phe Trp Ser Lys His Val Asp Gly Asp Gln Cys Leu Val Leu Pro 
                85                  90                  95      


Leu Glu His Pro Cys Ala Ser Leu Cys Cys Gly His Gly Thr Cys Ile 
            100                 105                 110         


Asp Gly Ile Gly Ser Phe Ser Cys Asp Cys Arg Ser Gly Trp Glu Gly 
        115                 120                 125             


Arg Phe Cys Gln Arg Glu Val Ser Phe Leu Asn Cys Ser Leu Asp Asn 
    130                 135                 140                 


Gly Gly Cys Thr His Tyr Cys Leu Glu Glu Val Gly Trp Arg Arg Cys 
145                 150                 155                 160 


Ser Cys Ala Pro Gly Tyr Lys Leu Gly Asp Asp Leu Leu Gln Cys His 
                165                 170                 175     


Pro Ala Val Lys Phe Pro Cys Gly Arg Pro Trp Lys Arg Met Glu Lys 
            180                 185                 190         


Lys Arg Ser His Leu Lys Arg Asp Thr Glu Asp Gln Glu Asp Gln Val 
        195                 200                 205             


Asp Pro Arg Leu Ile Asp Gly Lys Met Thr Arg Arg Gly Asp Ser Pro 
    210                 215                 220                 


Trp Gln Val Val Leu Leu Asp Ser Lys Lys Lys Leu Ala Cys Gly Ala 
225                 230                 235                 240 


Val Leu Ile His Pro Ser Trp Val Leu Thr Ala Ala His Cys Met Asp 
                245                 250                 255     


Glu Ser Lys Lys Leu Leu Val Arg Leu Gly Glu Tyr Asp Leu Arg Arg 
            260                 265                 270         


Trp Glu Lys Trp Glu Leu Asp Leu Asp Ile Lys Glu Val Phe Val His 
        275                 280                 285             


Pro Asn Tyr Ser Lys Ser Thr Thr Asp Asn Asp Ile Ala Leu Leu His 
    290                 295                 300                 


Leu Ala Gln Pro Ala Thr Leu Ser Gln Thr Ile Val Pro Ile Cys Leu 
305                 310                 315                 320 


Pro Asp Ser Gly Leu Ala Glu Arg Glu Leu Asn Gln Ala Gly Gln Glu 
                325                 330                 335     


Thr Leu Val Thr Gly Trp Gly Tyr His Ser Ser Arg Glu Lys Glu Ala 
            340                 345                 350         


Lys Arg Asn Arg Thr Phe Val Leu Asn Phe Ile Lys Ile Pro Val Val 
        355                 360                 365             


Pro His Asn Glu Cys Ser Glu Val Met Ser Asn Met Val Ser Glu Asn 
    370                 375                 380                 


Met Leu Cys Ala Gly Ile Leu Gly Asp Arg Gln Asp Ala Cys Glu Gly 
385                 390                 395                 400 


Asp Ser Gly Gly Pro Met Val Ala Ser Phe His Gly Thr Trp Phe Leu 
                405                 410                 415     


Val Gly Leu Val Ser Trp Gly Glu Gly Cys Gly Leu Leu His Asn Tyr 
            420                 425                 430         


Gly Val Tyr Thr Lys Val Ser Arg Tyr Leu Asp Trp Ile His Gly His 
        435                 440                 445             


Ile Arg Asp Lys Glu Ala Pro Gln Lys Ser Trp Ala Pro 
    450                 455                 460     


<210>  4
<211>  419
<212>  PRT
<213>  Homo sapiens

<400>  4

Ala Asn Ser Phe Leu Glu Glu Leu Arg His Ser Ser Leu Glu Arg Glu 
1               5                   10                  15      


Cys Ile Glu Glu Ile Cys Asp Phe Glu Glu Ala Lys Glu Ile Phe Gln 
            20                  25                  30          


Asn Val Asp Asp Thr Leu Ala Phe Trp Ser Lys His Val Asp Gly Asp 
        35                  40                  45              


Gln Cys Leu Val Leu Pro Leu Glu His Pro Cys Ala Ser Leu Cys Cys 
    50                  55                  60                  


Gly His Gly Thr Cys Ile Asp Gly Ile Gly Ser Phe Ser Cys Asp Cys 
65                  70                  75                  80  


Arg Ser Gly Trp Glu Gly Arg Phe Cys Gln Arg Glu Val Ser Phe Leu 
                85                  90                  95      


Asn Cys Ser Leu Asp Asn Gly Gly Cys Thr His Tyr Cys Leu Glu Glu 
            100                 105                 110         


Val Gly Trp Arg Arg Cys Ser Cys Ala Pro Gly Tyr Lys Leu Gly Asp 
        115                 120                 125             


Asp Leu Leu Gln Cys His Pro Ala Val Lys Phe Pro Cys Gly Arg Pro 
    130                 135                 140                 


Trp Lys Arg Met Glu Lys Lys Arg Ser His Leu Lys Arg Asp Thr Glu 
145                 150                 155                 160 


Asp Gln Glu Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys Met Thr 
                165                 170                 175     


Arg Arg Gly Asp Ser Pro Trp Gln Val Val Leu Leu Asp Ser Lys Lys 
            180                 185                 190         


Lys Leu Ala Cys Gly Ala Val Leu Ile His Pro Ser Trp Val Leu Thr 
        195                 200                 205             


Ala Ala His Cys Met Asp Glu Ser Lys Lys Leu Leu Val Arg Leu Gly 
    210                 215                 220                 


Glu Tyr Asp Leu Arg Arg Trp Glu Lys Trp Glu Leu Asp Leu Asp Ile 
225                 230                 235                 240 


Lys Glu Val Phe Val His Pro Asn Tyr Ser Lys Ser Thr Thr Asp Asn 
                245                 250                 255     


Asp Ile Ala Leu Leu His Leu Ala Gln Pro Ala Thr Leu Ser Gln Thr 
            260                 265                 270         


Ile Val Pro Ile Cys Leu Pro Asp Ser Gly Leu Ala Glu Arg Glu Leu 
        275                 280                 285             


Asn Gln Ala Gly Gln Glu Thr Leu Val Thr Gly Trp Gly Tyr His Ser 
    290                 295                 300                 


Ser Arg Glu Lys Glu Ala Lys Arg Asn Arg Thr Phe Val Leu Asn Phe 
305                 310                 315                 320 


Ile Lys Ile Pro Val Val Pro His Asn Glu Cys Ser Glu Val Met Ser 
                325                 330                 335     


Asn Met Val Ser Glu Asn Met Leu Cys Ala Gly Ile Leu Gly Asp Arg 
            340                 345                 350         


Gln Asp Ala Cys Glu Gly Asp Ser Gly Gly Pro Met Val Ala Ser Phe 
        355                 360                 365             


His Gly Thr Trp Phe Leu Val Gly Leu Val Ser Trp Gly Glu Gly Cys 
    370                 375                 380                 


Gly Leu Leu His Asn Tyr Gly Val Tyr Thr Lys Val Ser Arg Tyr Leu 
385                 390                 395                 400 


Asp Trp Ile His Gly His Ile Arg Asp Lys Glu Ala Pro Gln Lys Ser 
                405                 410                 415     


Trp Ala Pro 
            


<210>  5
<211>  455
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PCgla-egf1FVII chimeric zymogen


<220>
<221>  MISC_FEATURE
<222>  (1)..(133)
<223>  amino acid residues from PC

<220>
<221>  MISC_FEATURE
<222>  (134)..(455)
<223>  amino acid residues from FVII

<220>
<221>  MISC_FEATURE
<222>  (201)..(202)
<223>  FVII activation site

<400>  5

Met Trp Gln Leu Thr Ser Leu Leu Leu Phe Val Ala Thr Trp Gly Ile 
1               5                   10                  15      


Ser Gly Thr Pro Ala Pro Leu Asp Ser Val Phe Ser Ser Ser Glu Arg 
            20                  25                  30          


Ala His Gln Val Leu Arg Ile Arg Lys Arg Ala Asn Ser Phe Leu Glu 
        35                  40                  45              


Glu Leu Arg His Ser Ser Leu Glu Arg Glu Cys Ile Glu Glu Ile Cys 
    50                  55                  60                  


Asp Phe Glu Glu Ala Lys Glu Ile Phe Gln Asn Val Asp Asp Thr Leu 
65                  70                  75                  80  


Ala Phe Trp Ser Lys His Val Asp Gly Asp Gln Cys Leu Val Leu Pro 
                85                  90                  95      


Leu Glu His Pro Cys Ala Ser Leu Cys Cys Gly His Gly Thr Cys Ile 
            100                 105                 110         


Asp Gly Ile Gly Ser Phe Ser Cys Asp Cys Arg Ser Gly Trp Glu Gly 
        115                 120                 125             


Arg Phe Cys Gln Arg Lys Asp Asp Gln Leu Ile Cys Val Asn Glu Asn 
    130                 135                 140                 


Gly Gly Cys Glu Gln Tyr Cys Ser Asp His Thr Gly Thr Lys Arg Ser 
145                 150                 155                 160 


Cys Arg Cys His Glu Gly Tyr Ser Leu Leu Ala Asp Gly Val Ser Cys 
                165                 170                 175     


Thr Pro Thr Val Glu Tyr Pro Cys Gly Lys Ile Pro Ile Leu Glu Lys 
            180                 185                 190         


Arg Asn Ala Ser Lys Pro Gln Gly Arg Ile Val Gly Gly Lys Val Cys 
        195                 200                 205             


Pro Lys Gly Glu Cys Pro Trp Gln Val Leu Leu Leu Val Asn Gly Ala 
    210                 215                 220                 


Gln Leu Cys Gly Gly Thr Leu Ile Asn Thr Ile Trp Val Val Ser Ala 
225                 230                 235                 240 


Ala His Cys Phe Asp Lys Ile Lys Asn Trp Arg Asn Leu Ile Ala Val 
                245                 250                 255     


Leu Gly Glu His Asp Leu Ser Glu His Asp Gly Asp Glu Gln Ser Arg 
            260                 265                 270         


Arg Val Ala Gln Val Ile Ile Pro Ser Thr Tyr Val Pro Gly Thr Thr 
        275                 280                 285             


Asn His Asp Ile Ala Leu Leu Arg Leu His Gln Pro Val Val Leu Thr 
    290                 295                 300                 


Asp His Val Val Pro Leu Cys Leu Pro Glu Arg Thr Phe Ser Glu Arg 
305                 310                 315                 320 


Thr Leu Ala Phe Val Arg Phe Ser Leu Val Ser Gly Trp Gly Gln Leu 
                325                 330                 335     


Leu Asp Arg Gly Ala Thr Ala Leu Glu Leu Met Val Leu Asn Val Pro 
            340                 345                 350         


Arg Leu Met Thr Gln Asp Cys Leu Gln Gln Ser Arg Lys Val Gly Asp 
        355                 360                 365             


Ser Pro Asn Ile Thr Glu Tyr Met Phe Cys Ala Gly Tyr Ser Asp Gly 
    370                 375                 380                 


Ser Lys Asp Ser Cys Lys Gly Asp Ser Gly Gly Pro His Ala Thr His 
385                 390                 395                 400 


Tyr Arg Gly Thr Trp Tyr Leu Thr Gly Ile Val Ser Trp Gly Gln Gly 
                405                 410                 415     


Cys Ala Thr Val Gly His Phe Gly Val Tyr Thr Arg Val Ser Gln Tyr 
            420                 425                 430         


Ile Glu Trp Leu Gln Lys Leu Met Arg Ser Glu Pro Arg Pro Gly Val 
        435                 440                 445             


Leu Leu Arg Ala Pro Phe Pro 
    450                 455 


<210>  6
<211>  413
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PCgla-egf1FVIIa chimera


<220>
<221>  MISC_FEATURE
<222>  (1)..(91)
<223>  amino acid residues from PC

<220>
<221>  MISC_FEATURE
<222>  (92)..(413)
<223>  amino acid sequences from FVII

<220>
<221>  MISC_FEATURE
<222>  (159)..(160)
<223>  FVII activation site

<400>  6

Ala Asn Ser Phe Leu Glu Glu Leu Arg His Ser Ser Leu Glu Arg Glu 
1               5                   10                  15      


Cys Ile Glu Glu Ile Cys Asp Phe Glu Glu Ala Lys Glu Ile Phe Gln 
            20                  25                  30          


Asn Val Asp Asp Thr Leu Ala Phe Trp Ser Lys His Val Asp Gly Asp 
        35                  40                  45              


Gln Cys Leu Val Leu Pro Leu Glu His Pro Cys Ala Ser Leu Cys Cys 
    50                  55                  60                  


Gly His Gly Thr Cys Ile Asp Gly Ile Gly Ser Phe Ser Cys Asp Cys 
65                  70                  75                  80  


Arg Ser Gly Trp Glu Gly Arg Phe Cys Gln Arg Lys Asp Asp Gln Leu 
                85                  90                  95      


Ile Cys Val Asn Glu Asn Gly Gly Cys Glu Gln Tyr Cys Ser Asp His 
            100                 105                 110         


Thr Gly Thr Lys Arg Ser Cys Arg Cys His Glu Gly Tyr Ser Leu Leu 
        115                 120                 125             


Ala Asp Gly Val Ser Cys Thr Pro Thr Val Glu Tyr Pro Cys Gly Lys 
    130                 135                 140                 


Ile Pro Ile Leu Glu Lys Arg Asn Ala Ser Lys Pro Gln Gly Arg Ile 
145                 150                 155                 160 


Val Gly Gly Lys Val Cys Pro Lys Gly Glu Cys Pro Trp Gln Val Leu 
                165                 170                 175     


Leu Leu Val Asn Gly Ala Gln Leu Cys Gly Gly Thr Leu Ile Asn Thr 
            180                 185                 190         


Ile Trp Val Val Ser Ala Ala His Cys Phe Asp Lys Ile Lys Asn Trp 
        195                 200                 205             


Arg Asn Leu Ile Ala Val Leu Gly Glu His Asp Leu Ser Glu His Asp 
    210                 215                 220                 


Gly Asp Glu Gln Ser Arg Arg Val Ala Gln Val Ile Ile Pro Ser Thr 
225                 230                 235                 240 


Tyr Val Pro Gly Thr Thr Asn His Asp Ile Ala Leu Leu Arg Leu His 
                245                 250                 255     


Gln Pro Val Val Leu Thr Asp His Val Val Pro Leu Cys Leu Pro Glu 
            260                 265                 270         


Arg Thr Phe Ser Glu Arg Thr Leu Ala Phe Val Arg Phe Ser Leu Val 
        275                 280                 285             


Ser Gly Trp Gly Gln Leu Leu Asp Arg Gly Ala Thr Ala Leu Glu Leu 
    290                 295                 300                 


Met Val Leu Asn Val Pro Arg Leu Met Thr Gln Asp Cys Leu Gln Gln 
305                 310                 315                 320 


Ser Arg Lys Val Gly Asp Ser Pro Asn Ile Thr Glu Tyr Met Phe Cys 
                325                 330                 335     


Ala Gly Tyr Ser Asp Gly Ser Lys Asp Ser Cys Lys Gly Asp Ser Gly 
            340                 345                 350         


Gly Pro His Ala Thr His Tyr Arg Gly Thr Trp Tyr Leu Thr Gly Ile 
        355                 360                 365             


Val Ser Trp Gly Gln Gly Cys Ala Thr Val Gly His Phe Gly Val Tyr 
    370                 375                 380                 


Thr Arg Val Ser Gln Tyr Ile Glu Trp Leu Gln Lys Leu Met Arg Ser 
385                 390                 395                 400 


Glu Pro Arg Pro Gly Val Leu Leu Arg Ala Pro Phe Pro 
                405                 410             


<210>  7
<211>  1386
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA sequence encoding the PCgla-egf1FVII chimeric zymogen

<400>  7
gtggaattca tgtggcagct cacaagcctc ctgctgttcg tggccacctg gggaatttcc       60

ggcacaccag ctcctcttga ctcagtgttc tccagcagcg agcgtgccca ccaggtgctg      120

cggatccgca aacgtgccaa ctccttcctg gaggagctcc gtcacagcag cctggagcgg      180

gagtgcatag aggagatctg tgacttcgag gaggccaagg aaattttcca aaatgtggat      240

gacacactgg ccttctggtc caagcacgtc gacggtgacc agtgcttggt cttgcccttg      300

gagcacccgt gcgccagcct gtgctgcggg cacggcacgt gcatcgacgg catcggcagc      360

ttcagctgcg actgccgcag cggctgggag ggccgcttct gccagcgcaa ggatgaccag      420

ctgatctgtg tgaacgagaa cggcggctgt gagcagtact gcagtgacca cacgggcacc      480

aagcgctcct gtcggtgcca cgaggggtac tctctgctgg cagacggggt gtcctgcaca      540

cccacagttg aatatccatg tggaaaaata cctattctag aaaaaagaaa tgccagcaaa      600

ccccaaggcc gaattgtggg gggcaaggtg tgccccaaag gggagtgtcc atggcaggtc      660

ctgttgttgg tgaatggagc tcagttgtgt ggggggaccc tgatcaacac catctgggtg      720

gtctccgcgg cccactgttt cgacaaaatc aagaactgga ggaacctgat cgcggtgctg      780

ggcgagcacg acctcagcga gcacgacggg gatgagcaga gccggcgggt ggcgcaggtc      840

atcatcccca gcacgtacgt cccgggcacc accaaccacg acatcgcgct gctccgcctg      900

caccagcccg tggtcctcac tgaccatgtg gtgcccctct gcctgcccga acggacgttc      960

tctgagagga cgctggcctt cgtgcgcttc tcattggtca gcggctgggg ccagctgctg     1020

gaccgtggcg ccacggccct ggagctcatg gtcctcaacg tgccccggct gatgacccag     1080

gactgcctgc agcagtcacg gaaggtggga gactccccaa atatcacgga gtacatgttc     1140

tgtgccggct actcggatgg cagcaaggac tcctgcaagg gggacagtgg aggcccacat     1200

gccacccact accggggcac gtggtacctg acgggcatcg tcagctgggg ccagggctgc     1260

gcaaccgtgg gccactttgg ggtgtacacc agggtctccc agtacatcga gtggctgcaa     1320

aagctcatgc gctcagagcc acgcccagga gtcctcctgc gagccccatt tccctagctc     1380

gagtct                                                                1386


<210>  8
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FVII Gla domain

<400>  8

Ala Asn Ala Phe Leu Glu Glu Leu Arg Pro Gly Ser Leu Glu Arg Glu 
1               5                   10                  15      


Cys Lys Glu Glu Gln Cys Ser Phe Glu Glu Ala Arg Glu Ile Phe Lys 
            20                  25                  30          


Asp Ala Glu Arg Thr Lys Leu Phe Trp Ile Ser Tyr Ser 
        35                  40                  45  


<210>  9
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PC Gla domain

<400>  9

Ala Asn Ser Phe Leu Glu Glu Leu Arg His Ser Ser Leu Glu Arg Glu 
1               5                   10                  15      


Cys Ile Glu Glu Ile Cys Asp Phe Glu Glu Ala Lys Glu Ile Phe Gln 
            20                  25                  30          


Asn Val Asp Asp Thr Leu Ala Phe Trp Ser Lys His Val Asp 
        35                  40                  45      


<210>  10
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FVII EGF-1 domain

<400>  10

Asp Gly Asp Gln Cys Ala Ser Ser Pro Cys Gln Asn Gly Gly Ser Cys 
1               5                   10                  15      


Lys Asp Gln Leu Gln Ser Tyr Ile Cys Phe Cys Leu Pro Ala Phe Glu 
            20                  25                  30          


Gly Arg Asn Cys Glu 
        35          


<210>  11
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FVII EGF-2 domain

<400>  11

Asp Gln Leu Ile Cys Val Asn Glu Asn Gly Gly Cys Glu Gln Tyr Cys 
1               5                   10                  15      


Ser Asp His Thr Gly Thr Lys Arg Ser Cys Arg Cys His Glu Gly Tyr 
            20                  25                  30          


Ser Leu Leu Ala Asp Gly Val Ser Cys Thr 
        35                  40          


<210>  12
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PC EGF-1 domain

<400>  12

Leu Glu His Pro Cys Ala Ser Leu Cys Cys Gly His Gly Thr Cys Ile 
1               5                   10                  15      


Asp Gly Ile Gly Ser Phe Ser Cys Asp Cys Arg Ser Gly Trp Glu Gly 
            20                  25                  30          


Arg Phe Cys Gln 
        35      


<210>  13
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PC-EGF-2 domain

<400>  13

Ser Phe Leu Asn Cys Ser Leu Asp Asn Gly Gly Cys Thr His Tyr Cys 
1               5                   10                  15      


Leu Glu Glu Val Gly Trp Arg Arg Cys Ser Cys Ala Pro Gly Tyr Lys 
            20                  25                  30          


Leu Gly Asp Asp Leu Leu Gln Cys His 
        35                  40      


<210>  14
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FVII Protease domain

<400>  14

Ile Val Gly Gly Lys Val Cys Pro Lys Gly Glu Cys Pro Trp Gln Val 
1               5                   10                  15      


Leu Leu Leu Val Asn Gly Ala Gln Leu Cys Gly Gly Thr Leu Ile Asn 
            20                  25                  30          


Thr Ile Trp Val Val Ser Ala Ala His Cys Phe Asp Lys Ile Lys Asn 
        35                  40                  45              


Trp Arg Asn Leu Ile Ala Val Leu Gly Glu His Asp Leu Ser Glu His 
    50                  55                  60                  


Asp Gly Asp Glu Gln Ser Arg Arg Val Ala Gln Val Ile Ile Pro Ser 
65                  70                  75                  80  


Thr Tyr Val Pro Gly Thr Thr Asn His Asp Ile Ala Leu Leu Arg Leu 
                85                  90                  95      


His Gln Pro Val Val Leu Thr Asp His Val Val Pro Leu Cys Leu Pro 
            100                 105                 110         


Glu Arg Thr Phe Ser Glu Arg Thr Leu Ala Phe Val Arg Phe Ser Leu 
        115                 120                 125             


Val Ser Gly Trp Gly Gln Leu Leu Asp Arg Gly Ala Thr Ala Leu Glu 
    130                 135                 140                 


Leu Met Val Leu Asn Val Pro Arg Leu Met Thr Gln Asp Cys Leu Gln 
145                 150                 155                 160 


Gln Ser Arg Lys Val Gly Asp Ser Pro Asn Ile Thr Glu Tyr Met Phe 
                165                 170                 175     


Cys Ala Gly Tyr Ser Asp Gly Ser Lys Asp Ser Cys Lys Gly Asp Ser 
            180                 185                 190         


Gly Gly Pro His Ala Thr His Tyr Arg Gly Thr Trp Tyr Leu Thr Gly 
        195                 200                 205             


Ile Val Ser Trp Gly Gln Gly Cys Ala Thr Val Gly His Phe Gly Val 
    210                 215                 220                 


Tyr Thr Arg Val Ser Gln Tyr Ile Glu Trp Leu Gln Lys Leu Met Arg 
225                 230                 235                 240 


<210>  15
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FVII Protease domain

<400>  15

Ile Val Gly Gly Lys Val Cys Pro Lys Gly Glu Cys Pro Trp Gln Val 
1               5                   10                  15      


Leu Leu Leu Val Asn Gly Ala Gln Leu Cys Gly Gly Thr Leu Ile Asn 
            20                  25                  30          


Thr Ile Trp Val Val Ser Ala Ala His Cys Phe Asp Lys Ile Lys Asn 
        35                  40                  45              


Trp Arg Asn Leu Ile Ala Val Leu Gly Glu His Asp Leu Ser Glu His 
    50                  55                  60                  


Asp Gly Asp Glu Gln Ser Arg Arg Val Ala Gln Val Ile Ile Pro Ser 
65                  70                  75                  80  


Thr Tyr Val Pro Gly Thr Thr Asn His Asp Ile Ala Leu Leu Arg Leu 
                85                  90                  95      


His Gln Pro Val Val Leu Thr Asp His Val Val Pro Leu Cys Leu Pro 
            100                 105                 110         


Glu Arg Thr Phe Ser Glu Arg Thr Leu Ala Phe Val Arg Phe Ser Leu 
        115                 120                 125             


Val Ser Gly Trp Gly Gln Leu Leu Asp Arg Gly Ala Thr Ala Leu Glu 
    130                 135                 140                 


Leu Met Val Leu Asn Val Pro Arg Leu Met Thr Gln Asp Cys Leu Gln 
145                 150                 155                 160 


Gln Ser Arg Lys Val Gly Asp Ser Pro Asn Ile Thr Glu Tyr Met Phe 
                165                 170                 175     


Cys Ala Gly Tyr Ser Asp Gly Ser Lys Asp Ser Cys Lys Gly Asp Ser 
            180                 185                 190         


Gly Gly Pro His Ala Thr His Tyr Arg Gly Thr Trp Tyr Leu Thr Gly 
        195                 200                 205             


Ile Val Ser Trp Gly Gln Gly Cys Ala Thr Val Gly His Phe Gly Val 
    210                 215                 220                 


Tyr Thr Arg Val Ser Gln Tyr Ile Glu Trp Leu Gln Lys Leu Met Arg 
225                 230                 235                 240 


Ser Glu Pro Arg Pro Gly Val Leu Leu Arg Ala Pro Phe Pro 
                245                 250                 


<210>  16
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PC Protease domain

<400>  16

Leu Ile Asp Gly Lys Met Thr Arg Arg Gly Asp Ser Pro Trp Gln Val 
1               5                   10                  15      


Val Leu Leu Asp Ser Lys Lys Lys Leu Ala Cys Gly Ala Val Leu Ile 
            20                  25                  30          


His Pro Ser Trp Val Leu Thr Ala Ala His Cys Met Asp Glu Ser Lys 
        35                  40                  45              


Lys Leu Leu Val Arg Leu Gly Glu Tyr Asp Leu Arg Arg Trp Glu Lys 
    50                  55                  60                  


Trp Glu Leu Asp Leu Asp Ile Lys Glu Val Phe Val His Pro Asn Tyr 
65                  70                  75                  80  


Ser Lys Ser Thr Thr Asp Asn Asp Ile Ala Leu Leu His Leu Ala Gln 
                85                  90                  95      


Pro Ala Thr Leu Ser Gln Thr Ile Val Pro Ile Cys Leu Pro Asp Ser 
            100                 105                 110         


Gly Leu Ala Glu Arg Glu Leu Asn Gln Ala Gly Gln Glu Thr Leu Val 
        115                 120                 125             


Thr Gly Trp Gly Tyr His Ser Ser Arg Glu Lys Glu Ala Lys Arg Asn 
    130                 135                 140                 


Arg Thr Phe Val Leu Asn Phe Ile Lys Ile Pro Val Val Pro His Asn 
145                 150                 155                 160 


Glu Cys Ser Glu Val Met Ser Asn Met Val Ser Glu Asn Met Leu Cys 
                165                 170                 175     


Ala Gly Ile Leu Gly Asp Arg Gln Asp Ala Cys Glu Gly Asp Ser Gly 
            180                 185                 190         


Gly Pro Met Val Ala Ser Phe His Gly Thr Trp Phe Leu Val Gly Leu 
        195                 200                 205             


Val Ser Trp Gly Glu Gly Cys Gly Leu Leu His Asn Tyr Gly Val Tyr 
    210                 215                 220                 


Thr Lys Val Ser Arg Tyr Leu Asp Trp Ile His Gly His Ile Arg 
225                 230                 235                 


