
                                SEQUENCE LISTING

<110> Regents of the University of Minnesota

<120> CO-RECEPTOR AFFINITY ENHANCED MAJOR
  HISTOCOMPATIBILITY
  CLASS II MOLECULES

<130> 14483-20007.40

<140> Not Yet Assigned    
<141> Concurrently Herewith            

<150> US 62/713,388       
<151> 2018-08-01  

<160> 32

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 34
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 1
Trp Phe Arg Asn Gly Gln Glu Glu Thr Val Gly Val Ser Ser Thr Gln
 1               5                  10                  15      
Leu Ile Arg Asn Gly Asp Trp Thr Phe Gln Val Leu Val Met Leu Glu
            20                  25                  30          
Met Thr
        


<210> 2
<211> 34
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<220> 
<221> VARIANT        
<222> 7, 12, 18, 28
<223> Xaa = any one of the 20 standard amino acid residues

<400> 2
Trp Phe Arg Asn Gly Gln Xaa Glu Thr Val Gly Xaa Ser Ser Thr Gln
 1               5                  10                  15      
Leu Xaa Arg Asn Gly Asp Trp Thr Phe Gln Val Xaa Val Met Leu Glu
            20                  25                  30          
Met Thr
        


<210> 3
<211> 101
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<220> 
<221> misc_feature   
<222> 19, 20, 21, 34, 35, 36, 52, 53, 54, 81, 82, 83
<223> n = A,T,C or G

<400> 3
tggttccgga atggccagnn ngagacggtg gggnnntcat ccacacagct tnnnaggaat 60
gggactggac cttccaggtc nnngtcatgc tggacatgac c                     101

<210> 4
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 4
Trp Phe Arg Asn Gly Gln Glu Glu Thr Val Gly Val Ser Ser Thr Gln
 1               5                  10                  15      
Leu Ile Arg Asn Gly Asp Trp Thr Phe Gln Val Leu Val Met
            20                  25                  30  


<210> 5
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 5
Trp Phe Arg Asn Gly Gln Ala Glu Thr Val Gly Met Ser Ser Thr Gln
 1               5                  10                  15      
Leu Ile Arg Asn Gly Asp Trp Thr Phe Gln Val Trp Val Met
            20                  25                  30  


<210> 6
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 6
Trp Phe Arg Asn Gly Gln Glu Glu Thr Val Gly Ile Ser Ser Thr Gln
 1               5                  10                  15      
Leu Tyr Arg Asn Gly Asp Trp Thr Phe Gln Val Asp Val Met
            20                  25                  30  


<210> 7
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 7
Trp Phe Arg Asn Gly Lys Glu Glu Lys Thr Gly Ile Val Ser Thr Gly
 1               5                  10                  15      
Leu Val Arg Asn Gly Asp Trp Thr Phe Gln Thr Leu Val Met
            20                  25                  30  


<210> 8
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<220> 
<221> VARIANT        
<222> 6
<223> Xaa = Gln or Lys

<220> 
<221> VARIANT        
<222> 7
<223> Xaa = Glu or Ala

<220> 
<221> VARIANT        
<222> 9
<223> Xaa = Thr or Lys

<220> 
<221> VARIANT        
<222> 10
<223> Xaa = Val or Thr

<220> 
<221> VARIANT        
<222> 12
<223> Xaa = Val or Ile or Met

<220> 
<221> VARIANT        
<222> 13
<223> Xaa = Ser or Val

<220> 
<221> VARIANT        
<222> 16
<223> Xaa = Gln or Gly

<220> 
<221> VARIANT        
<222> 18
<223> Xaa = Ile or Val or Tyr

<220> 
<221> VARIANT        
<222> 27
<223> Xaa = Val or Thr

<220> 
<221> VARIANT        
<222> 28
<223> Xaa = Leu or Trp or Asp

<400> 8
Trp Phe Arg Asn Gly Xaa Xaa Glu Xaa Xaa Gly Xaa Xaa Ser Thr Xaa
 1               5                  10                  15      
Leu Xaa Arg Asn Gly Asp Trp Thr Phe Gln Xaa Xaa Val Met
            20                  25                  30  


<210> 9
<211> 237
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 9
Asp Ser Glu Arg His Phe Val Tyr Gln Phe Met Gly Glu Cys Tyr Phe
 1               5                  10                  15      
Thr Asn Gly Thr Gln Arg Ile Arg Tyr Val Thr Arg Tyr Ile Tyr Asn
            20                  25                  30          
Arg Glu Glu Tyr Val Arg Tyr Asp Ser Asp Val Gly Glu His Arg Ala
        35                  40                  45              
Val Thr Glu Leu Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Pro
    50                  55                  60                  
Glu Ile Leu Glu Arg Thr Arg Ala Glu Leu Asp Thr Val Cys Arg His
65                  70                  75                  80  
Asn Tyr Glu Gly Pro Glu Thr His Thr Ser Leu Arg Arg Leu Glu Gln
                85                  90                  95      
Pro Asn Val Val Ile Ser Leu Ser Arg Thr Glu Ala Leu Asn His His
            100                 105                 110         
Asn Thr Leu Val Cys Ser Val Thr Asp Phe Tyr Pro Ala Lys Ile Lys
        115                 120                 125             
Val Arg Trp Phe Arg Asn Gly Gln Glu Glu Thr Val Gly Val Ser Ser
    130                 135                 140                 
Thr Gln Leu Ile Arg Asn Gly Asp Trp Thr Phe Gln Val Leu Val Met
145                 150                 155                 160 
Leu Glu Met Thr Pro Arg Arg Gly Glu Val Tyr Thr Cys His Val Glu
                165                 170                 175     
His Pro Ser Leu Lys Ser Pro Ile Thr Val Glu Trp Arg Ala Gln Ser
            180                 185                 190         
Glu Ser Ala Trp Ser Lys Met Leu Ser Gly Ile Gly Gly Cys Val Leu
        195                 200                 205             
Gly Val Ile Phe Leu Gly Leu Gly Leu Phe Ile Arg His Arg Ser Gln
    210                 215                 220                 
Lys Gly Pro Arg Gly Pro Pro Pro Ala Gly Leu Leu Gln
225                 230                 235         


<210> 10
<211> 850
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 10
gtttaaactt aagcttatgg ctctgcagat ccccagcctc ctcctctcag ctgctgtggt 60
ggtgctgatg gtgctgagca gccccgggac tgagggcgga gattccgagg cccagaaggc 120
ccgcgccaac aaggccgtgg acaaggccgg cggcggaggt actagtggcg gtggaagtgg 180
agggtctgaa aggcatttcg tgtaccagtt catgggcgag tgctacttca ccaacgggac 240
gcagcgcata cgatatgtga ccagatacat ctacaaccgg gaggagtacg tgcgctacga 300
cagcgacgtg ggcgagcacc gcgcggtgac cgagctgggg cggccagacg ccgagtactg 360
gaacagccag ccggagatcc tggagcgaac gcgggccgag ctggacacgg tgtgcagaca 420
caactacgag gggccggaga cccacacctc cctgcggcgg cttgaacagc ccaatgtcgt 480
catctccctg tccaggacag aggccctcaa ccaccacaac actctggtct gctcagtgac 540
agatttctac ccagccaaga tcaaagtgcg ctggttccgg aatggccaga tgcatgtcat 600
gctggagatg acccctcggc ggggagaggt ctacacctgt cacgtggagc atcccagcct 660
gaagagcccc atcactgtgg agtggagggc acagtctgag tctgcctgga gcaagatgtt 720
gagcggcatc gggggctgcg tgcttggggt gatcttcctc gggcttggcc ttttcatccg 780
tcacaggagt cagaaaggac ctcgaggccc tcctccagca gggctcctgc agtgaagctt 840
ggtaccgagc                                                        850

<210> 11
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 11
gtcatgctgg agatgacccc tcgg                                        24

<210> 12
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 12
ctggccattc cggaaccagc g                                           21

<210> 13
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 13
gtcatctcca gcatgac                                                17

<210> 14
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 14
cccccgaatt catgtgccga gccatctctc tta                              33

<210> 15
<211> 117
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 15
cccccgtcga cttaatgatg atgatgatga tgttcatgcc attcaatttt ctgcgcttca 60
aaaatatcgt tcaggccgct gccgccgctg ccgcctgtct ggttcacccc tctggat    117

<210> 16
<211> 296
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 16
Met Ala Leu Gln Ile Pro Ser Leu Leu Leu Ser Ala Ala Val Val Val
 1               5                  10                  15      
Leu Met Val Leu Ser Ser Pro Gly Thr Glu Gly Glu Ala Gln Lys Ala
            20                  25                  30          
Arg Ala Asn Lys Ala Val Asp Lys Ala Gly Gly Gly Gly Thr Ser Gly
        35                  40                  45              
Gly Gly Ser Gly Gly Ser Glu Arg His Phe Val Tyr Gln Phe Met Gly
    50                  55                  60                  
Glu Cys Tyr Phe Thr Asn Gly Thr Gln Arg Ile Arg Tyr Val Thr Arg
65                  70                  75                  80  
Tyr Ile Tyr Asn Arg Glu Glu Tyr Val Arg Tyr Asp Ser Asp Val Gly
                85                  90                  95      
Glu His Arg Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Glu Tyr Trp
            100                 105                 110         
Asn Ser Gln Pro Glu Ile Leu Glu Arg Thr Arg Ala Glu Leu Asp Thr
        115                 120                 125             
Val Cys Arg His Asn Tyr Glu Gly Pro Glu Thr His Thr Ser Leu Arg
    130                 135                 140                 
Arg Leu Glu Gln Pro Asn Val Val Ile Ser Leu Ser Arg Thr Glu Ala
145                 150                 155                 160 
Leu Asn His His Asn Thr Leu Val Cys Ser Val Thr Asp Phe Tyr Pro
                165                 170                 175     
Ala Lys Ile Lys Val Arg Trp Phe Arg Asn Gly Gln Glu Glu Thr Val
            180                 185                 190         
Gly Val Ser Ser Thr Gln Leu Ile Arg Asn Gly Asp Trp Thr Phe Gln
        195                 200                 205             
Val Leu Val Met Leu Glu Met Thr Pro Arg Arg Gly Glu Val Tyr Thr
    210                 215                 220                 
Cys His Val Glu His Pro Ser Leu Lys Ser Pro Ile Thr Val Glu Trp
225                 230                 235                 240 
Arg Ala Gln Ser Glu Ser Ala Trp Ser Lys Gly Gly Gly Gly Ser Thr
                245                 250                 255     
Thr Ala Pro Ser Ala Gln Leu Lys Lys Lys Leu Gln Ala Leu Lys Lys
            260                 265                 270         
Lys Asn Ala Gln Leu Lys Trp Lys Leu Gln Ala Leu Lys Lys Lys Leu
        275                 280                 285             
Ala Gln His His His His His His
    290                 295     


<210> 17
<211> 280
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 17
Met Pro Cys Ser Arg Ala Leu Ile Leu Gly Val Leu Ala Leu Thr Thr
 1               5                  10                  15      
Met Leu Ser Leu Cys Gly Gly Glu Asp Asp Ile Glu Ala Asp His Val
            20                  25                  30          
Gly Thr Tyr Gly Ile Ser Val Tyr Gln Ser Pro Gly Asp Ile Gly Gln
        35                  40                  45              
Tyr Thr Phe Glu Phe Asp Gly Asp Glu Leu Phe Tyr Val Asp Leu Asp
    50                  55                  60                  
Lys Lys Glu Thr Val Trp Met Leu Pro Glu Phe Gly Gln Leu Ala Ser
65                  70                  75                  80  
Phe Asp Pro Gln Gly Gly Leu Gln Asn Ile Ala Val Val Lys His Asn
                85                  90                  95      
Leu Gly Val Leu Thr Lys Arg Ser Asn Ser Thr Pro Ala Thr Asn Glu
            100                 105                 110         
Ala Pro Gln Ala Thr Val Phe Pro Lys Ser Pro Val Leu Leu Gly Gln
        115                 120                 125             
Pro Asn Thr Leu Ile Cys Phe Val Asp Asn Ile Phe Pro Pro Val Ile
    130                 135                 140                 
Asn Ile Thr Trp Leu Arg Asn Ser Lys Ser Val Ala Asp Gly Val Tyr
145                 150                 155                 160 
Glu Thr Ser Phe Phe Val Asn Arg Asp Tyr Ser Phe His Lys Leu Ser
                165                 170                 175     
Tyr Leu Thr Phe Ile Pro Ser Asp Asp Asp Ile Tyr Asp Cys Lys Val
            180                 185                 190         
Glu His Trp Gly Leu Glu Glu Pro Val Leu Lys His Trp Glu Pro Glu
        195                 200                 205             
Ile Pro Ala Pro Met Ser Glu Leu Thr Glu Thr Gly Gly Gly Gly Ser
    210                 215                 220                 
Thr Thr Ala Pro Ser Ala Gln Leu Glu Lys Glu Leu Gln Ala Leu Glu
225                 230                 235                 240 
Lys Glu Asn Ala Gln Leu Glu Trp Glu Leu Gln Ala Leu Glu Lys Glu
                245                 250                 255     
Leu Ala Gln Gly Gly Ser Gly Gly Ser Gly Leu Asn Asp Ile Phe Glu
            260                 265                 270         
Ala Gln Lys Ile Glu Trp His Glu
        275                 280 


<210> 18
<211> 27
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 18
Met Ala Leu Gln Ile Pro Ser Leu Leu Leu Ser Ala Ala Val Val Val
 1               5                  10                  15      
Leu Met Val Leu Ser Ser Pro Gly Thr Glu Gly
            20                  25          


<210> 19
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 19
Glu Ala Gln Lys Ala Arg Ala Asn Lys Ala Val Asp Lys Ala
 1               5                  10                  


<210> 20
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 20
Gly Gly Gly Gly Thr Ser Gly Gly Gly Ser Gly Gly Ser
 1               5                  10              


<210> 21
<211> 196
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 21
Glu Arg His Phe Val Tyr Gln Phe Met Gly Glu Cys Tyr Phe Thr Asn
 1               5                  10                  15      
Gly Thr Gln Arg Ile Arg Tyr Val Thr Arg Tyr Ile Tyr Asn Arg Glu
            20                  25                  30          
Glu Tyr Val Arg Tyr Asp Ser Asp Val Gly Glu His Arg Ala Val Thr
        35                  40                  45              
Glu Leu Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Pro Glu Ile
    50                  55                  60                  
Leu Glu Arg Thr Arg Ala Glu Leu Asp Thr Val Cys Arg His Asn Tyr
65                  70                  75                  80  
Glu Gly Pro Glu Thr His Thr Ser Leu Arg Arg Leu Glu Gln Pro Asn
                85                  90                  95      
Val Val Ile Ser Leu Ser Arg Thr Glu Ala Leu Asn His His Asn Thr
            100                 105                 110         
Leu Val Cys Ser Val Thr Asp Phe Tyr Pro Ala Lys Ile Lys Val Arg
        115                 120                 125             
Trp Phe Arg Asn Gly Gln Glu Glu Thr Val Gly Val Ser Ser Thr Gln
    130                 135                 140                 
Leu Ile Arg Asn Gly Asp Trp Thr Phe Gln Val Leu Val Met Leu Glu
145                 150                 155                 160 
Met Thr Pro Arg Arg Gly Glu Val Tyr Thr Cys His Val Glu His Pro
                165                 170                 175     
Ser Leu Lys Ser Pro Ile Thr Val Glu Trp Arg Ala Gln Ser Glu Ser
            180                 185                 190         
Ala Trp Ser Lys
        195     


<210> 22
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 22
Gly Gly Gly Gly Ser
 1               5  


<210> 23
<211> 35
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 23
Thr Thr Ala Pro Ser Ala Gln Leu Lys Lys Lys Leu Gln Ala Leu Lys
 1               5                  10                  15      
Lys Lys Asn Ala Gln Leu Lys Trp Lys Leu Gln Ala Leu Lys Lys Lys
            20                  25                  30          
Leu Ala Gln
        35  


<210> 24
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 24
His His His His His His
 1               5      


<210> 25
<211> 23
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 25
Met Pro Cys Ser Arg Ala Leu Ile Leu Gly Val Leu Ala Leu Thr Thr
 1               5                  10                  15      
Met Leu Ser Leu Cys Gly Gly
            20              


<210> 26
<211> 196
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 26
Glu Asp Asp Ile Glu Ala Asp His Val Gly Thr Tyr Gly Ile Ser Val
 1               5                  10                  15      
Tyr Gln Ser Pro Gly Asp Ile Gly Gln Tyr Thr Phe Glu Phe Asp Gly
            20                  25                  30          
Asp Glu Leu Phe Tyr Val Asp Leu Asp Lys Lys Glu Thr Val Trp Met
        35                  40                  45              
Leu Pro Glu Phe Gly Gln Leu Ala Ser Phe Asp Pro Gln Gly Gly Leu
    50                  55                  60                  
Gln Asn Ile Ala Val Val Lys His Asn Leu Gly Val Leu Thr Lys Arg
65                  70                  75                  80  
Ser Asn Ser Thr Pro Ala Thr Asn Glu Ala Pro Gln Ala Thr Val Phe
                85                  90                  95      
Pro Lys Ser Pro Val Leu Leu Gly Gln Pro Asn Thr Leu Ile Cys Phe
            100                 105                 110         
Val Asp Asn Ile Phe Pro Pro Val Ile Asn Ile Thr Trp Leu Arg Asn
        115                 120                 125             
Ser Lys Ser Val Ala Asp Gly Val Tyr Glu Thr Ser Phe Phe Val Asn
    130                 135                 140                 
Arg Asp Tyr Ser Phe His Lys Leu Ser Tyr Leu Thr Phe Ile Pro Ser
145                 150                 155                 160 
Asp Asp Asp Ile Tyr Asp Cys Lys Val Glu His Trp Gly Leu Glu Glu
                165                 170                 175     
Pro Val Leu Lys His Trp Glu Pro Glu Ile Pro Ala Pro Met Ser Glu
            180                 185                 190         
Leu Thr Glu Thr
        195     


<210> 27
<211> 35
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 27
Thr Thr Ala Pro Ser Ala Gln Leu Glu Lys Glu Leu Gln Ala Leu Glu
 1               5                  10                  15      
Lys Glu Asn Ala Gln Leu Glu Trp Glu Leu Gln Ala Leu Glu Lys Glu
            20                  25                  30          
Leu Ala Gln
        35  


<210> 28
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 28
Gly Gly Ser Gly Gly Ser
 1               5      


<210> 29
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 29
Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu
 1               5                  10                  15  


<210> 30
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 30
Glu Ala Trp Gly Ala Leu Ala Asn Trp Ala Val Asp Ser Ala
 1               5                  10                  


<210> 31
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 31
Gly Cys Gly Gly Thr Ser Gly Gly Gly Ser Gly Gly Ser
 1               5                  10              


<210> 32
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 32
Gly Trp Tyr Arg Ser Pro Phe Ser Arg Val Val
 1               5                  10      

