SEQUENCE LISTING

<110>  MONTANA MOLECULAR, LLC
       HUGHES, THOMAS
       TEWSON, PAUL
 
<120>  BIOSENSORS FOR DETECTING ARRESTIN SIGNALING 

<130>  615319: 102098-004PC

<140>
<141>

<150>  US 62/712,176 
<151>  2018-07-30

<160>  61    

<170>  PatentIn version 3.5

<210>  1
<211>  7100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 1A plasmid


<220>
<221>  misc_feature
<222>  (320)..(699)
<223>  CMV enhancer

<220>
<221>  misc_feature
<222>  (700)..(903)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (1936)..(2664)
<223>  cpmNeonGreen

<220>
<221>  misc_feature
<222>  (2665)..(2667)
<223>  linker

<220>
<221>  misc_feature
<222>  (2665)..(3888)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (2665)..(3888)
<223>  bGH poly(A)signal

<220>
<221>  misc_feature
<222>  (4849)..(5437)
<223>  ori

<220>
<221>  misc_feature
<222>  (5980)..(6795)
<223>  KanR

<400>  1
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg ggtgaaatac cgcacagatg cgtaaggaga aaataccgca tcagattggc      240

tattggccat tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt      300

ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg      360

gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc      420

ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc      480

atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact      540

gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat      600

gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact      660

tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac      720

atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac      780

gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac      840

tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga      900

gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat      960

agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt     1020

ccccgtgcca agagtgacgt aagtaccgcc tatagactct ataggcacac ccctttggct     1080

cttatgcatg ctatactgtt tttggcttgg ggcctataca cccccgcttc cttatgctat     1140

aggtgatggt atagcttagc ctataggtgt gggttattga ccattattga ccactcccct     1200

attggtgacg atactttcca ttactaatcc ataacatggc tctttgccac aactatctct     1260

attggctata tgccaatact ctgtccttca gagactgaca cggactctgt atttttacag     1320

gatggggtcc catttattat ttacaaattc acatatacaa caacgccgtc ccccgtgccc     1380

gcagttttta ttaaacatag cgtgggatct ccacgcgaat ctcgggtacg tgttccggac     1440

atgggctctt ctccggtagc ggcggagctt ccacatccga gccctggtcc catgcctcca     1500

gcggctcatg gtcgctcggc agctccttgc tcctaacagt ggaggccaga cttaggcaca     1560

gcacaatgcc caccaccacc agtgtgccgc acaaggccgt ggcggtaggg tatgtgtctg     1620

aaaatgagcg tggagattgg gctcgcacgg ctgacgcaga tggaagactt aaggcagcgg     1680

cagaagaaga tgcaggcagc tgagttgttg tattctgata agagtcagag gtaactcccg     1740

ttgcggtgct gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc     1800

gcgccaccag acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct     1860

gcagtcaccg tcgtcgacac gtgtgatcag atatacgact cactataggg agacccaagc     1920

tggctagcgt ttaccatgtc gaagaagact taccccaacg acaaaaccat catcagtacc     1980

tttaagtgga gttacaccac tggaaatggc aagcgctacc ggagcactgc gcggaccacc     2040

tacacctttg ccaagccaat ggcggctaac tatctgaaga accagccgat gtacgtgttc     2100

cgtaagacgg agctcaagca ctccaagacc gagctcaact tcaaggagtg gcaaaaggcc     2160

tttaccgatg tgatgggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg     2220

agcaagggcg aggaggataa catggcctct ctcccagcga cacatgagtt acacatcttt     2280

ggctccatca acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa tccaaatgat     2340

ggttatgagg agttaaacct gaagtccacc aagggtgacc tccagttctc cccctggatt     2400

ctggtccctc atatcgggta tggcttccat cagtacctgc cctaccctga cgggatgtcg     2460

cctttccagg ccgccatggt agatggctcc ggataccaag tccatcgcac aatgcagttt     2520

gaagatggtg cctcccttac tgttaactac cgctacacct acgagggaag ccacatcaaa     2580

ggagaggccc aggtgaaggg gactggtttc cctgctgacg gtcctgtgat gaccaactcg     2640

ctgaccgctg cggactggtg caggggggag aaacccggga ccagggtctt caagaagtcg     2700

agccctaact gcaagctcac cgtgtacttg ggcaagcggg acttcgtaga tcacctggac     2760

aaagtggacc ctgtagatgg cgtggtgctt gtggaccctg actacctgaa ggaccgcaaa     2820

gtgtttgtga ccctcacctg cgccttccgc tatggccgtg aagacctgga tgtgctgggc     2880

ttgtccttcc gcaaagacct gttcatcgcc acctaccagg ccttcccccc ggtgcccaac     2940

ccaccccggc cccccacccg cctgcaggac cggctgctga ggaagctggg ccagcatgcc     3000

caccccttct tcttcaccat accccagaat cttccatgct ccgtcacact gcagccaggc     3060

ccagaggata caggaaaggc ctgcggcgta gactttgaga ttcgagcctt ctgtgctaaa     3120

tcactagaag agaaaagcca caaaaggaac tctgtgcggc tggtgatccg aaaggtgcag     3180

ttcgccccgg agaaacccgg cccccagcct tcagccgaaa ccacacgcca cttcctcatg     3240

tctgaccggt ccctgcacct cgaggcttcc ctggacaagg agctgtacta ccatggggag     3300

cccctcaatg taaatgtcca cgtcaccaac aactccacca agaccgtcaa gaagatcaaa     3360

gtctctgtga gacagtacgc cgacatctgc ctcttcagca ccgcccagta caagtgtcct     3420

gtggctcaac tcgaacaaga tgaccaggta tctcccagct ccacattctg taaggtgtac     3480

accataaccc cactgctcag cgacaaccgg gagaagcggg gtctcgccct ggatgggaaa     3540

ctcaagcacg aggacaccaa cctggcttcc agcaccatcg tgaaggaggg tgccaacaag     3600

gaggtgctgg gaatcctggt gtcctacagg gtcaaggtga agctggtggt gtctcgaggc     3660

ggggatgtct ctgtggagct gccttttgtt cttatgcacc ccaagcccca cgaccacatc     3720

cccctcccca gaccccagtc agccgctccg gagacagatg tccctgtgga caccaacctc     3780

attgaatttg ataccaacta tgccacagat gatgacattg tgtttgagga ctttgcccgg     3840

cttcggctga aggggatgaa ggatgacgac tatgatgatc aactctgcta gcgcgccctc     3900

gactgtgcct tctagttgcc agccatctgt atcgcggccg ctctagacca ggcgcctgga     3960

tccagatcac ttctggctaa taaaagatca gagctctaga gatctgtgtg ttggtttttt     4020

gtggatctgc tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt     4080

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat     4140

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag cacagcaagg     4200

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atgggtacct     4260

ctctctctct ctctctctct ctctctctct ctctctctcg gtacctctct ctctctctct     4320

ctctctctct ctctctctct ctctcggtac caggtgctga agaattgacc cggttcctcc     4380

tgggccagaa agaagcaggc acatcccctt ctctgtgaca caccctgtcc acgcccctgg     4440

ttcttagttc cagccccact cataggacac tcatagctca ggagggctcc gccttcaatc     4500

ccacccgcta aagtacttgg agcggtctct ccctccctca tcagcccacc aaaccaaacc     4560

tagcctccaa gagtgggaag aaattaaagc aagataggct attaagtgca gagggagaga     4620

aaatgcctcc aacatgtgag gaagtaatga gagaaatcat agaatttctt ccgcttcctc     4680

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     4740

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     4800

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     4860

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     4920

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     4980

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     5040

tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     5100

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     5160

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     5220

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     5280

cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     5340

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     5400

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac     5460

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc     5520

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     5580

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc     5640

agcgatctgt ctatttcgtt catccatagt tgcctgactc cggggggggg gggcgctgag     5700

gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc ccatcatcca     5760

gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga     5820

ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat     5880

ccttcaactc agcaaaagtt cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt     5940

aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat     6000

caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg     6060

tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta     6120

tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa     6180

aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa     6240

aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa     6300

atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac     6360

gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac     6420

tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc     6480

tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg     6540

cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt     6600

aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt     6660

cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata     6720

cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag acgtttcccg     6780

ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt     6840

tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg     6900

gctttccccc cccccccatt attgaagcat ttatcagggt tattgtctca tgagcggata     6960

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa     7020

agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg     7080

tatcacgagg ccctttcgtc                                                 7100


<210>  2
<211>  1953
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 1A coding sequence


<220>
<221>  misc_feature
<222>  (1)..(729)
<223>  cpmNeonGreen

<220>
<221>  misc_feature
<222>  (730)..(1953)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (730)..(732)
<223>  linker

<400>  2
atgtcgaaga agacttaccc caacgacaaa accatcatca gtacctttaa gtggagttac       60

accactggaa atggcaagcg ctaccggagc actgcgcgga ccacctacac ctttgccaag      120

ccaatggcgg ctaactatct gaagaaccag ccgatgtacg tgttccgtaa gacggagctc      180

aagcactcca agaccgagct caacttcaag gagtggcaaa aggcctttac cgatgtgatg      240

ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagcaa gggcgaggag      300

gataacatgg cctctctccc agcgacacat gagttacaca tctttggctc catcaacggt      360

gtggactttg acatggtggg tcagggcacc ggcaatccaa atgatggtta tgaggagtta      420

aacctgaagt ccaccaaggg tgacctccag ttctccccct ggattctggt ccctcatatc      480

gggtatggct tccatcagta cctgccctac cctgacggga tgtcgccttt ccaggccgcc      540

atggtagatg gctccggata ccaagtccat cgcacaatgc agtttgaaga tggtgcctcc      600

cttactgtta actaccgcta cacctacgag ggaagccaca tcaaaggaga ggcccaggtg      660

aaggggactg gtttccctgc tgacggtcct gtgatgacca actcgctgac cgctgcggac      720

tggtgcaggg gggagaaacc cgggaccagg gtcttcaaga agtcgagccc taactgcaag      780

ctcaccgtgt acttgggcaa gcgggacttc gtagatcacc tggacaaagt ggaccctgta      840

gatggcgtgg tgcttgtgga ccctgactac ctgaaggacc gcaaagtgtt tgtgaccctc      900

acctgcgcct tccgctatgg ccgtgaagac ctggatgtgc tgggcttgtc cttccgcaaa      960

gacctgttca tcgccaccta ccaggccttc cccccggtgc ccaacccacc ccggcccccc     1020

acccgcctgc aggaccggct gctgaggaag ctgggccagc atgcccaccc cttcttcttc     1080

accatacccc agaatcttcc atgctccgtc acactgcagc caggcccaga ggatacagga     1140

aaggcctgcg gcgtagactt tgagattcga gccttctgtg ctaaatcact agaagagaaa     1200

agccacaaaa ggaactctgt gcggctggtg atccgaaagg tgcagttcgc cccggagaaa     1260

cccggccccc agccttcagc cgaaaccaca cgccacttcc tcatgtctga ccggtccctg     1320

cacctcgagg cttccctgga caaggagctg tactaccatg gggagcccct caatgtaaat     1380

gtccacgtca ccaacaactc caccaagacc gtcaagaaga tcaaagtctc tgtgagacag     1440

tacgccgaca tctgcctctt cagcaccgcc cagtacaagt gtcctgtggc tcaactcgaa     1500

caagatgacc aggtatctcc cagctccaca ttctgtaagg tgtacaccat aaccccactg     1560

ctcagcgaca accgggagaa gcggggtctc gccctggatg ggaaactcaa gcacgaggac     1620

accaacctgg cttccagcac catcgtgaag gagggtgcca acaaggaggt gctgggaatc     1680

ctggtgtcct acagggtcaa ggtgaagctg gtggtgtctc gaggcgggga tgtctctgtg     1740

gagctgcctt ttgttcttat gcaccccaag ccccacgacc acatccccct ccccagaccc     1800

cagtcagccg ctccggagac agatgtccct gtggacacca acctcattga atttgatacc     1860

aactatgcca cagatgatga cattgtgttt gaggactttg cccggcttcg gctgaagggg     1920

atgaaggatg acgactatga tgatcaactc tgc                                  1953


<210>  3
<211>  651
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 1A coding sequence


<220>
<221>  SITE
<222>  (1)..(243)
<223>  cpmNeonGreen

<220>
<221>  SITE
<222>  (244)..(651)
<223>  B-arrestin

<220>
<221>   SITE
<222>  (244)..(244)
<223>   linker

<400>  3

Met Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe 
1               5                   10                  15      


Lys Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala 
            20                  25                  30          


Arg Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys 
        35                  40                  45              


Asn Gln Pro Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys 
    50                  55                  60                  


Thr Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met 
65                  70                  75                  80  


Gly Met Asp Glu Leu Tyr Lys Gly Gly Thr Gly Gly Ser Met Val Ser 
                85                  90                  95      


Lys Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr His Glu Leu 
            100                 105                 110         


His Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met Val Gly Gln 
        115                 120                 125             


Gly Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn Leu Lys Ser 
    130                 135                 140                 


Thr Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val Pro His Ile 
145                 150                 155                 160 


Gly Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly Met Ser Pro 
                165                 170                 175     


Phe Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val His Arg Thr 
            180                 185                 190         


Met Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr Arg Tyr Thr 
        195                 200                 205             


Tyr Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys Gly Thr Gly 
    210                 215                 220                 


Phe Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr Ala Ala Asp 
225                 230                 235                 240 


Trp Cys Arg Gly Glu Lys Pro Gly Thr Arg Val Phe Lys Lys Ser Ser 
                245                 250                 255     


Pro Asn Cys Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp 
            260                 265                 270         


His Leu Asp Lys Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro 
        275                 280                 285             


Asp Tyr Leu Lys Asp Arg Lys Val Phe Val Thr Leu Thr Cys Ala Phe 
    290                 295                 300                 


Arg Tyr Gly Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys 
305                 310                 315                 320 


Asp Leu Phe Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val Pro Asn Pro 
                325                 330                 335     


Pro Arg Pro Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg Lys Leu Gly 
            340                 345                 350         


Gln His Ala His Pro Phe Phe Phe Thr Ile Pro Gln Asn Leu Pro Cys 
        355                 360                 365             


Ser Val Thr Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly 
    370                 375                 380                 


Val Asp Phe Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu Glu Glu Lys 
385                 390                 395                 400 


Ser His Lys Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Phe 
                405                 410                 415     


Ala Pro Glu Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr Thr Arg His 
            420                 425                 430         


Phe Leu Met Ser Asp Arg Ser Leu His Leu Glu Ala Ser Leu Asp Lys 
        435                 440                 445             


Glu Leu Tyr Tyr His Gly Glu Pro Leu Asn Val Asn Val His Val Thr 
    450                 455                 460                 


Asn Asn Ser Thr Lys Thr Val Lys Lys Ile Lys Val Ser Val Arg Gln 
465                 470                 475                 480 


Tyr Ala Asp Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys Cys Pro Val 
                485                 490                 495     


Ala Gln Leu Glu Gln Asp Asp Gln Val Ser Pro Ser Ser Thr Phe Cys 
            500                 505                 510         


Lys Val Tyr Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg Glu Lys Arg 
        515                 520                 525             


Gly Leu Ala Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala 
    530                 535                 540                 


Ser Ser Thr Ile Val Lys Glu Gly Ala Asn Lys Glu Val Leu Gly Ile 
545                 550                 555                 560 


Leu Val Ser Tyr Arg Val Lys Val Lys Leu Val Val Ser Arg Gly Gly 
                565                 570                 575     


Asp Val Ser Val Glu Leu Pro Phe Val Leu Met His Pro Lys Pro His 
            580                 585                 590         


Asp His Ile Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro Glu Thr Asp 
        595                 600                 605             


Val Pro Val Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn Tyr Ala Thr 
    610                 615                 620                 


Asp Asp Asp Ile Val Phe Glu Asp Phe Ala Arg Leu Arg Leu Lys Gly 
625                 630                 635                 640 


Met Lys Asp Asp Asp Tyr Asp Asp Gln Leu Cys 
                645                 650     


<210>  4
<211>  729
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 1A -cpmNeonGreen


<220>
<221>  misc_feature
<222>  (1)..(729)
<223>  cpmNeonGreen

<400>  4
atgtcgaaga agacttaccc caacgacaaa accatcatca gtacctttaa gtggagttac       60

accactggaa atggcaagcg ctaccggagc actgcgcgga ccacctacac ctttgccaag      120

ccaatggcgg ctaactatct gaagaaccag ccgatgtacg tgttccgtaa gacggagctc      180

aagcactcca agaccgagct caacttcaag gagtggcaaa aggcctttac cgatgtgatg      240

ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagcaa gggcgaggag      300

gataacatgg cctctctccc agcgacacat gagttacaca tctttggctc catcaacggt      360

gtggactttg acatggtggg tcagggcacc ggcaatccaa atgatggtta tgaggagtta      420

aacctgaagt ccaccaaggg tgacctccag ttctccccct ggattctggt ccctcatatc      480

gggtatggct tccatcagta cctgccctac cctgacggga tgtcgccttt ccaggccgcc      540

atggtagatg gctccggata ccaagtccat cgcacaatgc agtttgaaga tggtgcctcc      600

cttactgtta actaccgcta cacctacgag ggaagccaca tcaaaggaga ggcccaggtg      660

aaggggactg gtttccctgc tgacggtcct gtgatgacca actcgctgac cgctgcggac      720

tggtgcagg                                                              729


<210>  5
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 1A - cpmNeonGreen

<400>  5

Met Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe 
1               5                   10                  15      


Lys Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala 
            20                  25                  30          


Arg Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys 
        35                  40                  45              


Asn Gln Pro Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys 
    50                  55                  60                  


Thr Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met 
65                  70                  75                  80  


Gly Met Asp Glu Leu Tyr Lys Gly Gly Thr Gly Gly Ser Met Val Ser 
                85                  90                  95      


Lys Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr His Glu Leu 
            100                 105                 110         


His Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met Val Gly Gln 
        115                 120                 125             


Gly Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn Leu Lys Ser 
    130                 135                 140                 


Thr Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val Pro His Ile 
145                 150                 155                 160 


Gly Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly Met Ser Pro 
                165                 170                 175     


Phe Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val His Arg Thr 
            180                 185                 190         


Met Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr Arg Tyr Thr 
        195                 200                 205             


Tyr Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys Gly Thr Gly 
    210                 215                 220                 


Phe Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr Ala Ala Asp 
225                 230                 235                 240 


Trp Cys Arg 
            


<210>  6
<211>  1224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 1A - Beta-arrestin-2

<400>  6
ggggagaaac ccgggaccag ggtcttcaag aagtcgagcc ctaactgcaa gctcaccgtg       60

tacttgggca agcgggactt cgtagatcac ctggacaaag tggaccctgt agatggcgtg      120

gtgcttgtgg accctgacta cctgaaggac cgcaaagtgt ttgtgaccct cacctgcgcc      180

ttccgctatg gccgtgaaga cctggatgtg ctgggcttgt ccttccgcaa agacctgttc      240

atcgccacct accaggcctt ccccccggtg cccaacccac cccggccccc cacccgcctg      300

caggaccggc tgctgaggaa gctgggccag catgcccacc ccttcttctt caccataccc      360

cagaatcttc catgctccgt cacactgcag ccaggcccag aggatacagg aaaggcctgc      420

ggcgtagact ttgagattcg agccttctgt gctaaatcac tagaagagaa aagccacaaa      480

aggaactctg tgcggctggt gatccgaaag gtgcagttcg ccccggagaa acccggcccc      540

cagccttcag ccgaaaccac acgccacttc ctcatgtctg accggtccct gcacctcgag      600

gcttccctgg acaaggagct gtactaccat ggggagcccc tcaatgtaaa tgtccacgtc      660

accaacaact ccaccaagac cgtcaagaag atcaaagtct ctgtgagaca gtacgccgac      720

atctgcctct tcagcaccgc ccagtacaag tgtcctgtgg ctcaactcga acaagatgac      780

caggtatctc ccagctccac attctgtaag gtgtacacca taaccccact gctcagcgac      840

aaccgggaga agcggggtct cgccctggat gggaaactca agcacgagga caccaacctg      900

gcttccagca ccatcgtgaa ggagggtgcc aacaaggagg tgctgggaat cctggtgtcc      960

tacagggtca aggtgaagct ggtggtgtct cgaggcgggg atgtctctgt ggagctgcct     1020

tttgttctta tgcaccccaa gccccacgac cacatccccc tccccagacc ccagtcagcc     1080

gctccggaga cagatgtccc tgtggacacc aacctcattg aatttgatac caactatgcc     1140

acagatgatg acattgtgtt tgaggacttt gcccggcttc ggctgaaggg gatgaaggat     1200

gacgactatg atgatcaact ctgc                                            1224


<210>  7
<211>  408
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 1A - Beta-arrestin-2


<220>
<221>  SITE
<222>  (1)..(408)
<223>  Beta-arrestin-2

<400>  7

Met Glu Lys Pro Gly Thr Arg Val Phe Lys Lys Ser Ser Pro Asn Cys 
1               5                   10                  15      


Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp His Leu Asp 
            20                  25                  30          


Lys Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro Asp Tyr Leu 
        35                  40                  45              


Lys Asp Arg Lys Val Phe Val Thr Leu Thr Cys Ala Phe Arg Tyr Gly 
    50                  55                  60                  


Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys Asp Leu Phe 
65                  70                  75                  80  


Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val Pro Asn Pro Pro Arg Pro 
                85                  90                  95      


Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg Lys Leu Gly Gln His Ala 
            100                 105                 110         


His Pro Phe Phe Phe Thr Ile Pro Gln Asn Leu Pro Cys Ser Val Thr 
        115                 120                 125             


Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp Phe 
    130                 135                 140                 


Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu Glu Glu Lys Ser His Lys 
145                 150                 155                 160 


Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Phe Ala Pro Glu 
                165                 170                 175     


Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr Thr Arg His Phe Leu Met 
            180                 185                 190         


Ser Asp Arg Ser Leu His Leu Glu Ala Ser Leu Asp Lys Glu Leu Tyr 
        195                 200                 205             


Tyr His Gly Glu Pro Leu Asn Val Asn Val His Val Thr Asn Asn Ser 
    210                 215                 220                 


Thr Lys Thr Val Lys Lys Ile Lys Val Ser Val Arg Gln Tyr Ala Asp 
225                 230                 235                 240 


Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys Cys Pro Val Ala Gln Leu 
                245                 250                 255     


Glu Gln Asp Asp Gln Val Ser Pro Ser Ser Thr Phe Cys Lys Val Tyr 
            260                 265                 270         


Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg Glu Lys Arg Gly Leu Ala 
        275                 280                 285             


Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser Thr 
    290                 295                 300                 


Ile Val Lys Glu Gly Ala Asn Lys Glu Val Leu Gly Ile Leu Val Ser 
305                 310                 315                 320 


Tyr Arg Val Lys Val Lys Leu Val Val Ser Arg Gly Gly Asp Val Ser 
                325                 330                 335     


Val Glu Leu Pro Phe Val Leu Met His Pro Lys Pro His Asp His Ile 
            340                 345                 350         


Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro Glu Thr Asp Val Pro Val 
        355                 360                 365             


Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn Tyr Ala Thr Asp Asp Asp 
    370                 375                 380                 


Ile Val Phe Glu Asp Phe Ala Arg Leu Arg Leu Lys Gly Met Lys Asp 
385                 390                 395                 400 


Asp Asp Tyr Asp Asp Gln Leu Cys 
                405             


<210>  8
<211>  7100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 3D plasmid


<220>
<221>  misc_feature
<222>  (320)..(699)
<223>  CMV enhancer

<220>
<221>  misc_feature
<222>  (700)..(903)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (1039)..(1864)
<223>  CMV intron A

<220>
<221>  misc_feature
<222>  (1936)..(3162)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (3163)..(3171)
<223>  linker

<220>
<221>  misc_feature
<222>  (3172)..(3888)
<223>  cpmNeon

<220>
<221>  misc_feature
<222>  (4030)..(4254)
<223>  bGH poly(A) signal

<220>
<221>  misc_feature
<222>  (4849)..(5437)
<223>  ori

<220>
<221>  misc_feature
<222>  (5980)..(6795)
<223>  KanR

<400>  8
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg ggtgaaatac cgcacagatg cgtaaggaga aaataccgca tcagattggc      240

tattggccat tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt      300

ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg      360

gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc      420

ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc      480

atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact      540

gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat      600

gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact      660

tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac      720

atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac      780

gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac      840

tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga      900

gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat      960

agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt     1020

ccccgtgcca agagtgacgt aagtaccgcc tatagactct ataggcacac ccctttggct     1080

cttatgcatg ctatactgtt tttggcttgg ggcctataca cccccgcttc cttatgctat     1140

aggtgatggt atagcttagc ctataggtgt gggttattga ccattattga ccactcccct     1200

attggtgacg atactttcca ttactaatcc ataacatggc tctttgccac aactatctct     1260

attggctata tgccaatact ctgtccttca gagactgaca cggactctgt atttttacag     1320

gatggggtcc catttattat ttacaaattc acatatacaa caacgccgtc ccccgtgccc     1380

gcagttttta ttaaacatag cgtgggatct ccacgcgaat ctcgggtacg tgttccggac     1440

atgggctctt ctccggtagc ggcggagctt ccacatccga gccctggtcc catgcctcca     1500

gcggctcatg gtcgctcggc agctccttgc tcctaacagt ggaggccaga cttaggcaca     1560

gcacaatgcc caccaccacc agtgtgccgc acaaggccgt ggcggtaggg tatgtgtctg     1620

aaaatgagcg tggagattgg gctcgcacgg ctgacgcaga tggaagactt aaggcagcgg     1680

cagaagaaga tgcaggcagc tgagttgttg tattctgata agagtcagag gtaactcccg     1740

ttgcggtgct gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc     1800

gcgccaccag acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct     1860

gcagtcaccg tcgtcgacac gtgtgatcag atatacgact cactataggg agacccaagc     1920

tggctagcgt ttaccatggg ggagaaaccc gggaccaggg tcttcaagaa gtcgagccct     1980

aactgcaagc tcaccgtgta cttgggcaag cgggacttcg tagatcacct ggacaaagtg     2040

gaccctgtag atggcgtggt gcttgtggac cctgactacc tgaaggaccg caaagtgttt     2100

gtgaccctca cctgcgcctt ccgctatggc cgtgaagacc tggatgtgct gggcttgtcc     2160

ttccgcaaag acctgttcat cgccacctac caggccttcc ccccggtgcc caacccaccc     2220

cggcccccca cccgcctgca ggaccggctg ctgaggaagc tgggccagca tgcccacccc     2280

ttcttcttca ccatacccca gaatcttcca tgctccgtca cactgcagcc aggcccagag     2340

gatacaggaa aggcctgcgg cgtagacttt gagattcgag ccttctgtgc taaatcacta     2400

gaagagaaaa gccacaaaag gaactctgtg cggctggtga tccgaaaggt gcagttcgcc     2460

ccggagaaac ccggccccca gccttcagcc gaaaccacac gccacttcct catgtctgac     2520

cggtccctgc acctcgaggc ttccctggac aaggagctgt actaccatgg ggagcccctc     2580

aatgtaaatg tccacgtcac caacaactcc accaagaccg tcaagaagat caaagtctct     2640

gtgagacagt acgccgacat ctgcctcttc agcaccgccc agtacaagtg tcctgtggct     2700

caactcgaac aagatgacca ggtatctccc agctccacat tctgtaaggt gtacaccata     2760

accccactgc tcagcgacaa ccgggagaag cggggtctcg ccctggatgg gaaactcaag     2820

cacgaggaca ccaacctggc ttccagcacc atcgtgaagg agggtgccaa caaggaggtg     2880

ctgggaatcc tggtgtccta cagggtcaag gtgaagctgg tggtgtctcg aggcggggat     2940

gtctctgtgg agctgccttt tgttcttatg caccccaagc cccacgacca catccccctc     3000

cccagacccc agtcagccgc tccggagaca gatgtccctg tggacaccaa cctcattgaa     3060

tttgatacca actatgccac agatgatgac attgtgtttg aggactttgc ccggcttcgg     3120

ctgaagggga tgaaggatga cgactatgat gatcaactct gcccctcgca ttcgaagaag     3180

acttacccca acgacaaaac catcatcagt acctttaagt ggagttacac cactggaaat     3240

ggcaagcgct accggagcac tgcgcggacc acctacacct ttgccaagcc aatggcggct     3300

aactatctga agaaccagcc gatgtacgtg ttccgtaaga cggagctcaa gcactccaag     3360

accgagctca acttcaagga gtggcaaaag gcctttaccg atgtgatggg catggacgag     3420

ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg gcgaggagga taacatggcc     3480

tctctcccag cgacacatga gttacacatc tttggctcca tcaacggtgt ggactttgac     3540

atggtgggtc agggcaccgg caatccaaat gatggttatg aggagttaaa cctgaagtcc     3600

accaagggtg acctccagtt ctccccctgg attctggtcc ctcatatcgg gtatggcttc     3660

catcagtacc tgccctaccc tgacgggatg tcgcctttcc aggccgccat ggtagatggc     3720

tccggatacc aagtccatcg cacaatgcag tttgaagatg gtgcctccct tactgttaac     3780

taccgctaca cctacgaggg aagccacatc aaaggagagg cccaggtgaa ggggactggt     3840

ttccctgctg acggtcctgt gatgaccaac tcgctgaccg ctgcgtcgta gcgcgccctc     3900

gactgtgcct tctagttgcc agccatctgt atcgcggccg ctctagacca ggcgcctgga     3960

tccagatcac ttctggctaa taaaagatca gagctctaga gatctgtgtg ttggtttttt     4020

gtggatctgc tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt     4080

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat     4140

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag cacagcaagg     4200

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atgggtacct     4260

ctctctctct ctctctctct ctctctctct ctctctctcg gtacctctct ctctctctct     4320

ctctctctct ctctctctct ctctcggtac caggtgctga agaattgacc cggttcctcc     4380

tgggccagaa agaagcaggc acatcccctt ctctgtgaca caccctgtcc acgcccctgg     4440

ttcttagttc cagccccact cataggacac tcatagctca ggagggctcc gccttcaatc     4500

ccacccgcta aagtacttgg agcggtctct ccctccctca tcagcccacc aaaccaaacc     4560

tagcctccaa gagtgggaag aaattaaagc aagataggct attaagtgca gagggagaga     4620

aaatgcctcc aacatgtgag gaagtaatga gagaaatcat agaatttctt ccgcttcctc     4680

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     4740

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     4800

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     4860

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     4920

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     4980

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     5040

tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     5100

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     5160

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     5220

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     5280

cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     5340

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     5400

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac     5460

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc     5520

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     5580

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc     5640

agcgatctgt ctatttcgtt catccatagt tgcctgactc cggggggggg gggcgctgag     5700

gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc ccatcatcca     5760

gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga     5820

ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat     5880

ccttcaactc agcaaaagtt cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt     5940

aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat     6000

caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg     6060

tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta     6120

tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa     6180

aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa     6240

aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa     6300

atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac     6360

gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac     6420

tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc     6480

tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg     6540

cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt     6600

aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt     6660

cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata     6720

cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag acgtttcccg     6780

ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt     6840

tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg     6900

gctttccccc cccccccatt attgaagcat ttatcagggt tattgtctca tgagcggata     6960

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa     7020

agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg     7080

tatcacgagg ccctttcgtc                                                 7100


<210>  9
<211>  1953
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 3D coding sequence


<220>
<221>  misc_feature
<222>  (1)..(1227)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (1228)..(1236)
<223>  linker

<220>
<221>  misc_feature
<222>  (1237)..(1953)
<223>  cpmNeon

<400>  9
atgggggaga aacccgggac cagggtcttc aagaagtcga gccctaactg caagctcacc       60

gtgtacttgg gcaagcggga cttcgtagat cacctggaca aagtggaccc tgtagatggc      120

gtggtgcttg tggaccctga ctacctgaag gaccgcaaag tgtttgtgac cctcacctgc      180

gccttccgct atggccgtga agacctggat gtgctgggct tgtccttccg caaagacctg      240

ttcatcgcca cctaccaggc cttccccccg gtgcccaacc caccccggcc ccccacccgc      300

ctgcaggacc ggctgctgag gaagctgggc cagcatgccc accccttctt cttcaccata      360

ccccagaatc ttccatgctc cgtcacactg cagccaggcc cagaggatac aggaaaggcc      420

tgcggcgtag actttgagat tcgagccttc tgtgctaaat cactagaaga gaaaagccac      480

aaaaggaact ctgtgcggct ggtgatccga aaggtgcagt tcgccccgga gaaacccggc      540

ccccagcctt cagccgaaac cacacgccac ttcctcatgt ctgaccggtc cctgcacctc      600

gaggcttccc tggacaagga gctgtactac catggggagc ccctcaatgt aaatgtccac      660

gtcaccaaca actccaccaa gaccgtcaag aagatcaaag tctctgtgag acagtacgcc      720

gacatctgcc tcttcagcac cgcccagtac aagtgtcctg tggctcaact cgaacaagat      780

gaccaggtat ctcccagctc cacattctgt aaggtgtaca ccataacccc actgctcagc      840

gacaaccggg agaagcgggg tctcgccctg gatgggaaac tcaagcacga ggacaccaac      900

ctggcttcca gcaccatcgt gaaggagggt gccaacaagg aggtgctggg aatcctggtg      960

tcctacaggg tcaaggtgaa gctggtggtg tctcgaggcg gggatgtctc tgtggagctg     1020

ccttttgttc ttatgcaccc caagccccac gaccacatcc ccctccccag accccagtca     1080

gccgctccgg agacagatgt ccctgtggac accaacctca ttgaatttga taccaactat     1140

gccacagatg atgacattgt gtttgaggac tttgcccggc ttcggctgaa ggggatgaag     1200

gatgacgact atgatgatca actctgcccc tcgcattcga agaagactta ccccaacgac     1260

aaaaccatca tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg     1320

agcactgcgc ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac     1380

cagccgatgt acgtgttccg taagacggag ctcaagcact ccaagaccga gctcaacttc     1440

aaggagtggc aaaaggcctt taccgatgtg atgggcatgg acgagctgta caagggcggt     1500

accggaggga gcatggtgag caagggcgag gaggataaca tggcctctct cccagcgaca     1560

catgagttac acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc     1620

accggcaatc caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc     1680

cagttctccc cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc     1740

taccctgacg ggatgtcgcc tttccaggcc gccatggtag atggctccgg ataccaagtc     1800

catcgcacaa tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac     1860

gagggaagcc acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt     1920

cctgtgatga ccaactcgct gaccgctgcg tcg                                  1953


<210>  10
<211>  651
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 3d coding sequence


<220>
<221>  SITE
<222>  (1)..(409)
<223>  B-arrestin

<220>
<221>  SITE
<222>  (410)..(412)
<223>  linker

<220>
<221>  SITE
<222>  (413)..(651)
<223>  cpmNeon

<400>  10

Met Gly Glu Lys Pro Gly Thr Arg Val Phe Lys Lys Ser Ser Pro Asn 
1               5                   10                  15      


Cys Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp His Leu 
            20                  25                  30          


Asp Lys Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro Asp Tyr 
        35                  40                  45              


Leu Lys Asp Arg Lys Val Phe Val Thr Leu Thr Cys Ala Phe Arg Tyr 
    50                  55                  60                  


Gly Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys Asp Leu 
65                  70                  75                  80  


Phe Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val Pro Asn Pro Pro Arg 
                85                  90                  95      


Pro Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg Lys Leu Gly Gln His 
            100                 105                 110         


Ala His Pro Phe Phe Phe Thr Ile Pro Gln Asn Leu Pro Cys Ser Val 
        115                 120                 125             


Thr Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp 
    130                 135                 140                 


Phe Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu Glu Glu Lys Ser His 
145                 150                 155                 160 


Lys Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Phe Ala Pro 
                165                 170                 175     


Glu Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr Thr Arg His Phe Leu 
            180                 185                 190         


Met Ser Asp Arg Ser Leu His Leu Glu Ala Ser Leu Asp Lys Glu Leu 
        195                 200                 205             


Tyr Tyr His Gly Glu Pro Leu Asn Val Asn Val His Val Thr Asn Asn 
    210                 215                 220                 


Ser Thr Lys Thr Val Lys Lys Ile Lys Val Ser Val Arg Gln Tyr Ala 
225                 230                 235                 240 


Asp Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys Cys Pro Val Ala Gln 
                245                 250                 255     


Leu Glu Gln Asp Asp Gln Val Ser Pro Ser Ser Thr Phe Cys Lys Val 
            260                 265                 270         


Tyr Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg Glu Lys Arg Gly Leu 
        275                 280                 285             


Ala Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser 
    290                 295                 300                 


Thr Ile Val Lys Glu Gly Ala Asn Lys Glu Val Leu Gly Ile Leu Val 
305                 310                 315                 320 


Ser Tyr Arg Val Lys Val Lys Leu Val Val Ser Arg Gly Gly Asp Val 
                325                 330                 335     


Ser Val Glu Leu Pro Phe Val Leu Met His Pro Lys Pro His Asp His 
            340                 345                 350         


Ile Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro Glu Thr Asp Val Pro 
        355                 360                 365             


Val Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn Tyr Ala Thr Asp Asp 
    370                 375                 380                 


Asp Ile Val Phe Glu Asp Phe Ala Arg Leu Arg Leu Lys Gly Met Lys 
385                 390                 395                 400 


Asp Asp Asp Tyr Asp Asp Gln Leu Cys Pro Ser His Ser Lys Lys Thr 
                405                 410                 415     


Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys Trp Ser Tyr Thr 
            420                 425                 430         


Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg Thr Thr Tyr Thr 
        435                 440                 445             


Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn Gln Pro Met Tyr 
    450                 455                 460                 


Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr Glu Leu Asn Phe 
465                 470                 475                 480 


Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly Met Asp Glu Leu 
                485                 490                 495     


Tyr Lys Gly Gly Thr Gly Gly Ser Met Val Ser Lys Gly Glu Glu Asp 
            500                 505                 510         


Asn Met Ala Ser Leu Pro Ala Thr His Glu Leu His Ile Phe Gly Ser 
        515                 520                 525             


Ile Asn Gly Val Asp Phe Asp Met Val Gly Gln Gly Thr Gly Asn Pro 
    530                 535                 540                 


Asn Asp Gly Tyr Glu Glu Leu Asn Leu Lys Ser Thr Lys Gly Asp Leu 
545                 550                 555                 560 


Gln Phe Ser Pro Trp Ile Leu Val Pro His Ile Gly Tyr Gly Phe His 
                565                 570                 575     


Gln Tyr Leu Pro Tyr Pro Asp Gly Met Ser Pro Phe Gln Ala Ala Met 
            580                 585                 590         


Val Asp Gly Ser Gly Tyr Gln Val His Arg Thr Met Gln Phe Glu Asp 
        595                 600                 605             


Gly Ala Ser Leu Thr Val Asn Tyr Arg Tyr Thr Tyr Glu Gly Ser His 
    610                 615                 620                 


Ile Lys Gly Glu Ala Gln Val Lys Gly Thr Gly Phe Pro Ala Asp Gly 
625                 630                 635                 640 


Pro Val Met Thr Asn Ser Leu Thr Ala Ala Ser 
                645                 650     


<210>  11
<211>  1227
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 3D - Beta-arrestin-2


<220>
<221>  misc_feature
<222>  (1)..(1227)
<223>  B-Arrestin

<400>  11
atgggggaga aacccgggac cagggtcttc aagaagtcga gccctaactg caagctcacc       60

gtgtacttgg gcaagcggga cttcgtagat cacctggaca aagtggaccc tgtagatggc      120

gtggtgcttg tggaccctga ctacctgaag gaccgcaaag tgtttgtgac cctcacctgc      180

gccttccgct atggccgtga agacctggat gtgctgggct tgtccttccg caaagacctg      240

ttcatcgcca cctaccaggc cttccccccg gtgcccaacc caccccggcc ccccacccgc      300

ctgcaggacc ggctgctgag gaagctgggc cagcatgccc accccttctt cttcaccata      360

ccccagaatc ttccatgctc cgtcacactg cagccaggcc cagaggatac aggaaaggcc      420

tgcggcgtag actttgagat tcgagccttc tgtgctaaat cactagaaga gaaaagccac      480

aaaaggaact ctgtgcggct ggtgatccga aaggtgcagt tcgccccgga gaaacccggc      540

ccccagcctt cagccgaaac cacacgccac ttcctcatgt ctgaccggtc cctgcacctc      600

gaggcttccc tggacaagga gctgtactac catggggagc ccctcaatgt aaatgtccac      660

gtcaccaaca actccaccaa gaccgtcaag aagatcaaag tctctgtgag acagtacgcc      720

gacatctgcc tcttcagcac cgcccagtac aagtgtcctg tggctcaact cgaacaagat      780

gaccaggtat ctcccagctc cacattctgt aaggtgtaca ccataacccc actgctcagc      840

gacaaccggg agaagcgggg tctcgccctg gatgggaaac tcaagcacga ggacaccaac      900

ctggcttcca gcaccatcgt gaaggagggt gccaacaagg aggtgctggg aatcctggtg      960

tcctacaggg tcaaggtgaa gctggtggtg tctcgaggcg gggatgtctc tgtggagctg     1020

ccttttgttc ttatgcaccc caagccccac gaccacatcc ccctccccag accccagtca     1080

gccgctccgg agacagatgt ccctgtggac accaacctca ttgaatttga taccaactat     1140

gccacagatg atgacattgt gtttgaggac tttgcccggc ttcggctgaa ggggatgaag     1200

gatgacgact atgatgatca actctgc                                         1227


<210>  12
<211>  409
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 3D - Beta-arrestin-2


<220>
<221>  SITE
<222>  (1)..(409)
<223>  B-arrestin

<400>  12

Met Gly Glu Lys Pro Gly Thr Arg Val Phe Lys Lys Ser Ser Pro Asn 
1               5                   10                  15      


Cys Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp His Leu 
            20                  25                  30          


Asp Lys Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro Asp Tyr 
        35                  40                  45              


Leu Lys Asp Arg Lys Val Phe Val Thr Leu Thr Cys Ala Phe Arg Tyr 
    50                  55                  60                  


Gly Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys Asp Leu 
65                  70                  75                  80  


Phe Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val Pro Asn Pro Pro Arg 
                85                  90                  95      


Pro Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg Lys Leu Gly Gln His 
            100                 105                 110         


Ala His Pro Phe Phe Phe Thr Ile Pro Gln Asn Leu Pro Cys Ser Val 
        115                 120                 125             


Thr Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp 
    130                 135                 140                 


Phe Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu Glu Glu Lys Ser His 
145                 150                 155                 160 


Lys Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Phe Ala Pro 
                165                 170                 175     


Glu Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr Thr Arg His Phe Leu 
            180                 185                 190         


Met Ser Asp Arg Ser Leu His Leu Glu Ala Ser Leu Asp Lys Glu Leu 
        195                 200                 205             


Tyr Tyr His Gly Glu Pro Leu Asn Val Asn Val His Val Thr Asn Asn 
    210                 215                 220                 


Ser Thr Lys Thr Val Lys Lys Ile Lys Val Ser Val Arg Gln Tyr Ala 
225                 230                 235                 240 


Asp Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys Cys Pro Val Ala Gln 
                245                 250                 255     


Leu Glu Gln Asp Asp Gln Val Ser Pro Ser Ser Thr Phe Cys Lys Val 
            260                 265                 270         


Tyr Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg Glu Lys Arg Gly Leu 
        275                 280                 285             


Ala Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser 
    290                 295                 300                 


Thr Ile Val Lys Glu Gly Ala Asn Lys Glu Val Leu Gly Ile Leu Val 
305                 310                 315                 320 


Ser Tyr Arg Val Lys Val Lys Leu Val Val Ser Arg Gly Gly Asp Val 
                325                 330                 335     


Ser Val Glu Leu Pro Phe Val Leu Met His Pro Lys Pro His Asp His 
            340                 345                 350         


Ile Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro Glu Thr Asp Val Pro 
        355                 360                 365             


Val Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn Tyr Ala Thr Asp Asp 
    370                 375                 380                 


Asp Ile Val Phe Glu Asp Phe Ala Arg Leu Arg Leu Lys Gly Met Lys 
385                 390                 395                 400 


Asp Asp Asp Tyr Asp Asp Gln Leu Cys 
                405                 


<210>  13
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 3D - cpmNeonGreen


<220>
<221>  misc_feature
<222>  (1)..(717)
<223>  cpmNeonGreen

<400>  13
tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc       60

actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca      120

atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag      180

cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc      240

atggacgagc tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggaggat      300

aacatggcct ctctcccagc gacacatgag ttacacatct ttggctccat caacggtgtg      360

gactttgaca tggtgggtca gggcaccggc aatccaaatg atggttatga ggagttaaac      420

ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg      480

tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg      540

gtagatggct ccggatacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt      600

actgttaact accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgaag      660

gggactggtt tccctgctga cggtcctgtg atgaccaact cgctgaccgc tgcgtcg         717


<210>  14
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 3D - cpmNeonGreen

<400>  14

Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys 
1               5                   10                  15      


Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg 
            20                  25                  30          


Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn 
        35                  40                  45              


Gln Pro Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr 
    50                  55                  60                  


Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly 
65                  70                  75                  80  


Met Asp Glu Leu Tyr Lys Gly Gly Thr Gly Gly Ser Met Val Ser Lys 
                85                  90                  95      


Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr His Glu Leu His 
            100                 105                 110         


Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met Val Gly Gln Gly 
        115                 120                 125             


Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn Leu Lys Ser Thr 
    130                 135                 140                 


Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val Pro His Ile Gly 
145                 150                 155                 160 


Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly Met Ser Pro Phe 
                165                 170                 175     


Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val His Arg Thr Met 
            180                 185                 190         


Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr Arg Tyr Thr Tyr 
        195                 200                 205             


Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys Gly Thr Gly Phe 
    210                 215                 220                 


Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr Ala Ala Ser 
225                 230                 235                 


<210>  15
<211>  7100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 3F plasmid


<220>
<221>  misc_feature
<222>  (320)..(699)
<223>  CMV enhancer

<220>
<221>  misc_feature
<222>  (700)..(903)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (1039)..(1864)
<223>  CMV intron A

<220>
<221>  misc_feature
<222>  (1936)..(3162)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (3163)..(3171)
<223>  Critical linker sequence

<220>
<221>  misc_feature
<222>  (3172)..(3885)
<223>  cpmNeon

<220>
<221>  misc_feature
<222>  (4030)..(4254)
<223>  bGH poly(A) signal

<220>
<221>  misc_feature
<222>  (4849)..(5437)
<223>  ori

<220>
<221>  misc_feature
<222>  (5980)..(6795)
<223>  KanR

<400>  15
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg ggtgaaatac cgcacagatg cgtaaggaga aaataccgca tcagattggc      240

tattggccat tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt      300

ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg      360

gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc      420

ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc      480

atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact      540

gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat      600

gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact      660

tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac      720

atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac      780

gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac      840

tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga      900

gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat      960

agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt     1020

ccccgtgcca agagtgacgt aagtaccgcc tatagactct ataggcacac ccctttggct     1080

cttatgcatg ctatactgtt tttggcttgg ggcctataca cccccgcttc cttatgctat     1140

aggtgatggt atagcttagc ctataggtgt gggttattga ccattattga ccactcccct     1200

attggtgacg atactttcca ttactaatcc ataacatggc tctttgccac aactatctct     1260

attggctata tgccaatact ctgtccttca gagactgaca cggactctgt atttttacag     1320

gatggggtcc catttattat ttacaaattc acatatacaa caacgccgtc ccccgtgccc     1380

gcagttttta ttaaacatag cgtgggatct ccacgcgaat ctcgggtacg tgttccggac     1440

atgggctctt ctccggtagc ggcggagctt ccacatccga gccctggtcc catgcctcca     1500

gcggctcatg gtcgctcggc agctccttgc tcctaacagt ggaggccaga cttaggcaca     1560

gcacaatgcc caccaccacc agtgtgccgc acaaggccgt ggcggtaggg tatgtgtctg     1620

aaaatgagcg tggagattgg gctcgcacgg ctgacgcaga tggaagactt aaggcagcgg     1680

cagaagaaga tgcaggcagc tgagttgttg tattctgata agagtcagag gtaactcccg     1740

ttgcggtgct gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc     1800

gcgccaccag acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct     1860

gcagtcaccg tcgtcgacac gtgtgatcag atatacgact cactataggg agacccaagc     1920

tggctagcgt ttaccatggg ggagaaaccc gggaccaggg tcttcaagaa gtcgagccct     1980

aactgcaagc tcaccgtgta cttgggcaag cgggacttcg tagatcacct ggacaaagtg     2040

gaccctgtag atggcgtggt gcttgtggac cctgactacc tgaaggaccg caaagtgttt     2100

gtgaccctca cctgcgcctt ccgctatggc cgtgaagacc tggatgtgct gggcttgtcc     2160

ttccgcaaag acctgttcat cgccacctac caggccttcc ccccggtgcc caacccaccc     2220

cggcccccca cccgcctgca ggaccggctg ctgaggaagc tgggccagca tgcccacccc     2280

ttcttcttca ccatacccca gaatcttcca tgctccgtca cactgcagcc aggcccagag     2340

gatacaggaa aggcctgcgg cgtagacttt gagattcgag ccttctgtgc taaatcacta     2400

gaagagaaaa gccacaaaag gaactctgtg cggctggtga tccgaaaggt gcagttcgcc     2460

ccggagaaac ccggccccca gccttcagcc gaaaccacac gccacttcct catgtctgac     2520

cggtccctgc acctcgaggc ttccctggac aaggagctgt actaccatgg ggagcccctc     2580

aatgtaaatg tccacgtcac caacaactcc accaagaccg tcaagaagat caaagtctct     2640

gtgagacagt acgccgacat ctgcctcttc agcaccgccc agtacaagtg tcctgtggct     2700

caactcgaac aagatgacca ggtatctccc agctccacat tctgtaaggt gtacaccata     2760

accccactgc tcagcgacaa ccgggagaag cggggtctcg ccctggatgg gaaactcaag     2820

cacgaggaca ccaacctggc ttccagcacc atcgtgaagg agggtgccaa caaggaggtg     2880

ctgggaatcc tggtgtccta cagggtcaag gtgaagctgg tggtgtctcg aggcggggat     2940

gtctctgtgg agctgccttt tgttcttatg caccccaagc cccacgacca catccccctc     3000

cccagacccc agtcagccgc tccggagaca gatgtccctg tggacaccaa cctcattgaa     3060

tttgatacca actatgccac agatgatgac attgtgtttg aggactttgc ccggcttcgg     3120

ctgaagggga tgaaggatga cgactatgat gatcaactct gcccctcgca ttcgaagaag     3180

acttacccca acgacaaaac catcatcagt acctttaagt ggagttacac cactggaaat     3240

ggcaagcgct accggagcac tgcgcggacc acctacacct ttgccaagcc aatggcggct     3300

aactatctga agaaccagcc gatgtacgtg ttccgtaaga cggagctcaa gcactccaag     3360

accgagctca acttcaagga gtggcaaaag gcctttaccg atgtgatggg catggacgag     3420

ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg gcgaggagga taacatggcc     3480

tctctcccag cgacacatga gttacacatc tttggctcca tcaacggtgt ggactttgac     3540

atggtgggtc agggcaccgg caatccaaat gatggttatg aggagttaaa cctgaagtcc     3600

accaagggtg acctccagtt ctccccctgg attctggtcc ctcatatcgg gtatggcttc     3660

catcagtacc tgccctaccc tgacgggatg tcgcctttcc aggccgccat ggtagatggc     3720

tccggatacc aagtccatcg cacaatgcag tttgaagatg gtgcctccct tactgttaac     3780

taccgctaca cctacgaggg aagccacatc aaaggagagg cccaggtgaa ggggactggt     3840

ttccctgctg acggtcctgt gatgaccaac tcgctgaccg ctgcggacta gcgcgccctc     3900

gactgtgcct tctagttgcc agccatctgt atcgcggccg ctctagacca ggcgcctgga     3960

tccagatcac ttctggctaa taaaagatca gagctctaga gatctgtgtg ttggtttttt     4020

gtggatctgc tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt     4080

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat     4140

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag cacagcaagg     4200

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atgggtacct     4260

ctctctctct ctctctctct ctctctctct ctctctctcg gtacctctct ctctctctct     4320

ctctctctct ctctctctct ctctcggtac caggtgctga agaattgacc cggttcctcc     4380

tgggccagaa agaagcaggc acatcccctt ctctgtgaca caccctgtcc acgcccctgg     4440

ttcttagttc cagccccact cataggacac tcatagctca ggagggctcc gccttcaatc     4500

ccacccgcta aagtacttgg agcggtctct ccctccctca tcagcccacc aaaccaaacc     4560

tagcctccaa gagtgggaag aaattaaagc aagataggct attaagtgca gagggagaga     4620

aaatgcctcc aacatgtgag gaagtaatga gagaaatcat agaatttctt ccgcttcctc     4680

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     4740

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     4800

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     4860

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     4920

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     4980

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     5040

tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     5100

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     5160

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     5220

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     5280

cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     5340

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     5400

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac     5460

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc     5520

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     5580

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc     5640

agcgatctgt ctatttcgtt catccatagt tgcctgactc cggggggggg gggcgctgag     5700

gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc ccatcatcca     5760

gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga     5820

ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat     5880

ccttcaactc agcaaaagtt cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt     5940

aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat     6000

caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg     6060

tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta     6120

tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa     6180

aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa     6240

aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa     6300

atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac     6360

gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac     6420

tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc     6480

tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg     6540

cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt     6600

aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt     6660

cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata     6720

cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag acgtttcccg     6780

ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt     6840

tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg     6900

gctttccccc cccccccatt attgaagcat ttatcagggt tattgtctca tgagcggata     6960

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa     7020

agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg     7080

tatcacgagg ccctttcgtc                                                 7100


<210>  16
<211>  1950
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 3F coding sequence


<220>
<221>  misc_feature
<222>  (1)..(1227)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (1228)..(1236)
<223>  Critical linker sequence

<220>
<221>  misc_feature
<222>  (1237)..(1950)
<223>  cpmNeon

<400>  16
atgggggaga aacccgggac cagggtcttc aagaagtcga gccctaactg caagctcacc       60

gtgtacttgg gcaagcggga cttcgtagat cacctggaca aagtggaccc tgtagatggc      120

gtggtgcttg tggaccctga ctacctgaag gaccgcaaag tgtttgtgac cctcacctgc      180

gccttccgct atggccgtga agacctggat gtgctgggct tgtccttccg caaagacctg      240

ttcatcgcca cctaccaggc cttccccccg gtgcccaacc caccccggcc ccccacccgc      300

ctgcaggacc ggctgctgag gaagctgggc cagcatgccc accccttctt cttcaccata      360

ccccagaatc ttccatgctc cgtcacactg cagccaggcc cagaggatac aggaaaggcc      420

tgcggcgtag actttgagat tcgagccttc tgtgctaaat cactagaaga gaaaagccac      480

aaaaggaact ctgtgcggct ggtgatccga aaggtgcagt tcgccccgga gaaacccggc      540

ccccagcctt cagccgaaac cacacgccac ttcctcatgt ctgaccggtc cctgcacctc      600

gaggcttccc tggacaagga gctgtactac catggggagc ccctcaatgt aaatgtccac      660

gtcaccaaca actccaccaa gaccgtcaag aagatcaaag tctctgtgag acagtacgcc      720

gacatctgcc tcttcagcac cgcccagtac aagtgtcctg tggctcaact cgaacaagat      780

gaccaggtat ctcccagctc cacattctgt aaggtgtaca ccataacccc actgctcagc      840

gacaaccggg agaagcgggg tctcgccctg gatgggaaac tcaagcacga ggacaccaac      900

ctggcttcca gcaccatcgt gaaggagggt gccaacaagg aggtgctggg aatcctggtg      960

tcctacaggg tcaaggtgaa gctggtggtg tctcgaggcg gggatgtctc tgtggagctg     1020

ccttttgttc ttatgcaccc caagccccac gaccacatcc ccctccccag accccagtca     1080

gccgctccgg agacagatgt ccctgtggac accaacctca ttgaatttga taccaactat     1140

gccacagatg atgacattgt gtttgaggac tttgcccggc ttcggctgaa ggggatgaag     1200

gatgacgact atgatgatca actctgcccc tcgcattcga agaagactta ccccaacgac     1260

aaaaccatca tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg     1320

agcactgcgc ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac     1380

cagccgatgt acgtgttccg taagacggag ctcaagcact ccaagaccga gctcaacttc     1440

aaggagtggc aaaaggcctt taccgatgtg atgggcatgg acgagctgta caagggcggt     1500

accggaggga gcatggtgag caagggcgag gaggataaca tggcctctct cccagcgaca     1560

catgagttac acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc     1620

accggcaatc caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc     1680

cagttctccc cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc     1740

taccctgacg ggatgtcgcc tttccaggcc gccatggtag atggctccgg ataccaagtc     1800

catcgcacaa tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac     1860

gagggaagcc acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt     1920

cctgtgatga ccaactcgct gaccgctgcg                                      1950


<210>  17
<211>  650
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 3F coding sequence


<220>
<221>  SITE
<222>  (1)..(409)
<223>  B-arrestin

<220>
<221>  SITE
<222>  (410)..(412)
<223>  Critical linker sequence

<220>
<221>  SITE
<222>  (413)..(650)
<223>  cpmNeon

<400>  17

Met Gly Glu Lys Pro Gly Thr Arg Val Phe Lys Lys Ser Ser Pro Asn 
1               5                   10                  15      


Cys Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp His Leu 
            20                  25                  30          


Asp Lys Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro Asp Tyr 
        35                  40                  45              


Leu Lys Asp Arg Lys Val Phe Val Thr Leu Thr Cys Ala Phe Arg Tyr 
    50                  55                  60                  


Gly Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys Asp Leu 
65                  70                  75                  80  


Phe Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val Pro Asn Pro Pro Arg 
                85                  90                  95      


Pro Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg Lys Leu Gly Gln His 
            100                 105                 110         


Ala His Pro Phe Phe Phe Thr Ile Pro Gln Asn Leu Pro Cys Ser Val 
        115                 120                 125             


Thr Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp 
    130                 135                 140                 


Phe Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu Glu Glu Lys Ser His 
145                 150                 155                 160 


Lys Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Phe Ala Pro 
                165                 170                 175     


Glu Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr Thr Arg His Phe Leu 
            180                 185                 190         


Met Ser Asp Arg Ser Leu His Leu Glu Ala Ser Leu Asp Lys Glu Leu 
        195                 200                 205             


Tyr Tyr His Gly Glu Pro Leu Asn Val Asn Val His Val Thr Asn Asn 
    210                 215                 220                 


Ser Thr Lys Thr Val Lys Lys Ile Lys Val Ser Val Arg Gln Tyr Ala 
225                 230                 235                 240 


Asp Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys Cys Pro Val Ala Gln 
                245                 250                 255     


Leu Glu Gln Asp Asp Gln Val Ser Pro Ser Ser Thr Phe Cys Lys Val 
            260                 265                 270         


Tyr Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg Glu Lys Arg Gly Leu 
        275                 280                 285             


Ala Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser 
    290                 295                 300                 


Thr Ile Val Lys Glu Gly Ala Asn Lys Glu Val Leu Gly Ile Leu Val 
305                 310                 315                 320 


Ser Tyr Arg Val Lys Val Lys Leu Val Val Ser Arg Gly Gly Asp Val 
                325                 330                 335     


Ser Val Glu Leu Pro Phe Val Leu Met His Pro Lys Pro His Asp His 
            340                 345                 350         


Ile Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro Glu Thr Asp Val Pro 
        355                 360                 365             


Val Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn Tyr Ala Thr Asp Asp 
    370                 375                 380                 


Asp Ile Val Phe Glu Asp Phe Ala Arg Leu Arg Leu Lys Gly Met Lys 
385                 390                 395                 400 


Asp Asp Asp Tyr Asp Asp Gln Leu Cys Pro Ser His Ser Lys Lys Thr 
                405                 410                 415     


Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys Trp Ser Tyr Thr 
            420                 425                 430         


Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg Thr Thr Tyr Thr 
        435                 440                 445             


Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn Gln Pro Met Tyr 
    450                 455                 460                 


Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr Glu Leu Asn Phe 
465                 470                 475                 480 


Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly Met Asp Glu Leu 
                485                 490                 495     


Tyr Lys Gly Gly Thr Gly Gly Ser Met Val Ser Lys Gly Glu Glu Asp 
            500                 505                 510         


Asn Met Ala Ser Leu Pro Ala Thr His Glu Leu His Ile Phe Gly Ser 
        515                 520                 525             


Ile Asn Gly Val Asp Phe Asp Met Val Gly Gln Gly Thr Gly Asn Pro 
    530                 535                 540                 


Asn Asp Gly Tyr Glu Glu Leu Asn Leu Lys Ser Thr Lys Gly Asp Leu 
545                 550                 555                 560 


Gln Phe Ser Pro Trp Ile Leu Val Pro His Ile Gly Tyr Gly Phe His 
                565                 570                 575     


Gln Tyr Leu Pro Tyr Pro Asp Gly Met Ser Pro Phe Gln Ala Ala Met 
            580                 585                 590         


Val Asp Gly Ser Gly Tyr Gln Val His Arg Thr Met Gln Phe Glu Asp 
        595                 600                 605             


Gly Ala Ser Leu Thr Val Asn Tyr Arg Tyr Thr Tyr Glu Gly Ser His 
    610                 615                 620                 


Ile Lys Gly Glu Ala Gln Val Lys Gly Thr Gly Phe Pro Ala Asp Gly 
625                 630                 635                 640 


Pro Val Met Thr Asn Ser Leu Thr Ala Ala 
                645                 650 


<210>  18
<211>  1227
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 3F - Beta-arrestin-2


<220>
<221>  misc_feature
<222>  (1)..(1227)
<223>  B-arrestin

<400>  18
atgggggaga aacccgggac cagggtcttc aagaagtcga gccctaactg caagctcacc       60

gtgtacttgg gcaagcggga cttcgtagat cacctggaca aagtggaccc tgtagatggc      120

gtggtgcttg tggaccctga ctacctgaag gaccgcaaag tgtttgtgac cctcacctgc      180

gccttccgct atggccgtga agacctggat gtgctgggct tgtccttccg caaagacctg      240

ttcatcgcca cctaccaggc cttccccccg gtgcccaacc caccccggcc ccccacccgc      300

ctgcaggacc ggctgctgag gaagctgggc cagcatgccc accccttctt cttcaccata      360

ccccagaatc ttccatgctc cgtcacactg cagccaggcc cagaggatac aggaaaggcc      420

tgcggcgtag actttgagat tcgagccttc tgtgctaaat cactagaaga gaaaagccac      480

aaaaggaact ctgtgcggct ggtgatccga aaggtgcagt tcgccccgga gaaacccggc      540

ccccagcctt cagccgaaac cacacgccac ttcctcatgt ctgaccggtc cctgcacctc      600

gaggcttccc tggacaagga gctgtactac catggggagc ccctcaatgt aaatgtccac      660

gtcaccaaca actccaccaa gaccgtcaag aagatcaaag tctctgtgag acagtacgcc      720

gacatctgcc tcttcagcac cgcccagtac aagtgtcctg tggctcaact cgaacaagat      780

gaccaggtat ctcccagctc cacattctgt aaggtgtaca ccataacccc actgctcagc      840

gacaaccggg agaagcgggg tctcgccctg gatgggaaac tcaagcacga ggacaccaac      900

ctggcttcca gcaccatcgt gaaggagggt gccaacaagg aggtgctggg aatcctggtg      960

tcctacaggg tcaaggtgaa gctggtggtg tctcgaggcg gggatgtctc tgtggagctg     1020

ccttttgttc ttatgcaccc caagccccac gaccacatcc ccctccccag accccagtca     1080

gccgctccgg agacagatgt ccctgtggac accaacctca ttgaatttga taccaactat     1140

gccacagatg atgacattgt gtttgaggac tttgcccggc ttcggctgaa ggggatgaag     1200

gatgacgact atgatgatca actctgc                                         1227


<210>  19
<211>  409
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 3F - Beta-arrestin-2


<220>
<221>  misc_feature
<222>  (1)..(409)
<223>  B-arrestin

<400>  19

Met Gly Glu Lys Pro Gly Thr Arg Val Phe Lys Lys Ser Ser Pro Asn 
1               5                   10                  15      


Cys Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp His Leu 
            20                  25                  30          


Asp Lys Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro Asp Tyr 
        35                  40                  45              


Leu Lys Asp Arg Lys Val Phe Val Thr Leu Thr Cys Ala Phe Arg Tyr 
    50                  55                  60                  


Gly Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys Asp Leu 
65                  70                  75                  80  


Phe Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val Pro Asn Pro Pro Arg 
                85                  90                  95      


Pro Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg Lys Leu Gly Gln His 
            100                 105                 110         


Ala His Pro Phe Phe Phe Thr Ile Pro Gln Asn Leu Pro Cys Ser Val 
        115                 120                 125             


Thr Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp 
    130                 135                 140                 


Phe Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu Glu Glu Lys Ser His 
145                 150                 155                 160 


Lys Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Phe Ala Pro 
                165                 170                 175     


Glu Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr Thr Arg His Phe Leu 
            180                 185                 190         


Met Ser Asp Arg Ser Leu His Leu Glu Ala Ser Leu Asp Lys Glu Leu 
        195                 200                 205             


Tyr Tyr His Gly Glu Pro Leu Asn Val Asn Val His Val Thr Asn Asn 
    210                 215                 220                 


Ser Thr Lys Thr Val Lys Lys Ile Lys Val Ser Val Arg Gln Tyr Ala 
225                 230                 235                 240 


Asp Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys Cys Pro Val Ala Gln 
                245                 250                 255     


Leu Glu Gln Asp Asp Gln Val Ser Pro Ser Ser Thr Phe Cys Lys Val 
            260                 265                 270         


Tyr Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg Glu Lys Arg Gly Leu 
        275                 280                 285             


Ala Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser 
    290                 295                 300                 


Thr Ile Val Lys Glu Gly Ala Asn Lys Glu Val Leu Gly Ile Leu Val 
305                 310                 315                 320 


Ser Tyr Arg Val Lys Val Lys Leu Val Val Ser Arg Gly Gly Asp Val 
                325                 330                 335     


Ser Val Glu Leu Pro Phe Val Leu Met His Pro Lys Pro His Asp His 
            340                 345                 350         


Ile Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro Glu Thr Asp Val Pro 
        355                 360                 365             


Val Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn Tyr Ala Thr Asp Asp 
    370                 375                 380                 


Asp Ile Val Phe Glu Asp Phe Ala Arg Leu Arg Leu Lys Gly Met Lys 
385                 390                 395                 400 


Asp Asp Asp Tyr Asp Asp Gln Leu Cys 
                405                 


<210>  20
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 3F - cpmNeonGreen


<220>
<221>  misc_feature
<222>  (1)..(714)
<223>  cpmNeonGreen

<400>  20
tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc       60

actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca      120

atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag      180

cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc      240

atggacgagc tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggaggat      300

aacatggcct ctctcccagc gacacatgag ttacacatct ttggctccat caacggtgtg      360

gactttgaca tggtgggtca gggcaccggc aatccaaatg atggttatga ggagttaaac      420

ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg      480

tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg      540

gtagatggct ccggatacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt      600

actgttaact accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgaag      660

gggactggtt tccctgctga cggtcctgtg atgaccaact cgctgaccgc tgcg            714


<210>  21
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 3F - cpmNeonGreen


<220>
<221>  misc_feature
<222>  (1)..(238)
<223>  cpmNeonGreen

<400>  21

Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys 
1               5                   10                  15      


Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg 
            20                  25                  30          


Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn 
        35                  40                  45              


Gln Pro Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr 
    50                  55                  60                  


Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly 
65                  70                  75                  80  


Met Asp Glu Leu Tyr Lys Gly Gly Thr Gly Gly Ser Met Val Ser Lys 
                85                  90                  95      


Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr His Glu Leu His 
            100                 105                 110         


Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met Val Gly Gln Gly 
        115                 120                 125             


Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn Leu Lys Ser Thr 
    130                 135                 140                 


Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val Pro His Ile Gly 
145                 150                 155                 160 


Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly Met Ser Pro Phe 
                165                 170                 175     


Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val His Arg Thr Met 
            180                 185                 190         


Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr Arg Tyr Thr Tyr 
        195                 200                 205             


Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys Gly Thr Gly Phe 
    210                 215                 220                 


Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr Ala Ala 
225                 230                 235             


<210>  22
<211>  7079
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 4A plasmid


<220>
<221>  misc_feature
<222>  (320)..(699)
<223>  CMV enhancer

<220>
<221>  misc_feature
<222>  (321)..(904)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (1039)..(1864)
<223>  CMV intron

<220>
<221>  misc_feature
<222>  (1936)..(2385)
<223>  mNeonGreen

<220>
<221>  misc_feature
<222>  (2191)..(2200)
<223>  Kozak sequence

<220>
<221>  misc_feature
<222>  (2386)..(3609)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (2386)..(2388)
<223>  Met to Gly mutation

<220>
<221>  misc_feature
<222>  (3610)..(3870)
<223>  mNeonGreen

<220>
<221>  misc_feature
<222>  (4009)..(4233)
<223>  bGH poly(A) signal

<220>
<221>  misc_feature
<222>  (4828)..(5416)
<223>  ori

<220>
<221>  misc_feature
<222>  (5959)..(6774)
<223>  KanR

<400>  22
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg ggtgaaatac cgcacagatg cgtaaggaga aaataccgca tcagattggc      240

tattggccat tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt      300

ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg      360

gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc      420

ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc      480

atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact      540

gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat      600

gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact      660

tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac      720

atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac      780

gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac      840

tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga      900

gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat      960

agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt     1020

ccccgtgcca agagtgacgt aagtaccgcc tatagactct ataggcacac ccctttggct     1080

cttatgcatg ctatactgtt tttggcttgg ggcctataca cccccgcttc cttatgctat     1140

aggtgatggt atagcttagc ctataggtgt gggttattga ccattattga ccactcccct     1200

attggtgacg atactttcca ttactaatcc ataacatggc tctttgccac aactatctct     1260

attggctata tgccaatact ctgtccttca gagactgaca cggactctgt atttttacag     1320

gatggggtcc catttattat ttacaaattc acatatacaa caacgccgtc ccccgtgccc     1380

gcagttttta ttaaacatag cgtgggatct ccacgcgaat ctcgggtacg tgttccggac     1440

atgggctctt ctccggtagc ggcggagctt ccacatccga gccctggtcc catgcctcca     1500

gcggctcatg gtcgctcggc agctccttgc tcctaacagt ggaggccaga cttaggcaca     1560

gcacaatgcc caccaccacc agtgtgccgc acaaggccgt ggcggtaggg tatgtgtctg     1620

aaaatgagcg tggagattgg gctcgcacgg ctgacgcaga tggaagactt aaggcagcgg     1680

cagaagaaga tgcaggcagc tgagttgttg tattctgata agagtcagag gtaactcccg     1740

ttgcggtgct gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc     1800

gcgccaccag acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct     1860

gcagtcaccg tcgtcgacac gtgtgatcag atatacgact cactataggg agacccaagc     1920

tggctagcgt ttaccatggt gagcaagggc gaggaggata acatggcctc tctcccagcg     1980

acacatgagt tacacatctt tggctccatc aacggtgtgg actttgacat ggtgggtcag     2040

ggcaccggca atccaaatga tggttatgag gagttaaacc tgaagtccac caagggtgac     2100

ctccagttct ccccctggat tctggtccct catatcgggt atggcttcca tcagtacctg     2160

ccctaccctg acgggatgtc gcctttccag gccgccatgg tagatggctc cggataccaa     2220

gtccatcgca caatgcagtt tgaagatggt gcctccctta ctgttaacta ccgctacacc     2280

tacgagggaa gccacatcaa aggagaggcc caggtgaagg ggactggttt ccctgctgac     2340

ggtcctgtga tgaccaactc gctgaccgct gcggactggt gcagggggga gaaacccggg     2400

accagggtct tcaagaagtc gagccctaac tgcaagctca ccgtgtactt gggcaagcgg     2460

gacttcgtag atcacctgga caaagtggac cctgtagatg gcgtggtgct tgtggaccct     2520

gactacctga aggaccgcaa agtgtttgtg accctcacct gcgccttccg ctatggccgt     2580

gaagacctgg atgtgctggg cttgtccttc cgcaaagacc tgttcatcgc cacctaccag     2640

gccttccccc cggtgcccaa cccaccccgg ccccccaccc gcctgcagga ccggctgctg     2700

aggaagctgg gccagcatgc ccaccccttc ttcttcacca taccccagaa tcttccatgc     2760

tccgtcacac tgcagccagg cccagaggat acaggaaagg cctgcggcgt agactttgag     2820

attcgagcct tctgtgctaa atcactagaa gagaaaagcc acaaaaggaa ctctgtgcgg     2880

ctggtgatcc gaaaggtgca gttcgccccg gagaaacccg gcccccagcc ttcagccgaa     2940

accacacgcc acttcctcat gtctgaccgg tccctgcacc tcgaggcttc cctggacaag     3000

gagctgtact accatgggga gcccctcaat gtaaatgtcc acgtcaccaa caactccacc     3060

aagaccgtca agaagatcaa agtctctgtg agacagtacg ccgacatctg cctcttcagc     3120

accgcccagt acaagtgtcc tgtggctcaa ctcgaacaag atgaccaggt atctcccagc     3180

tccacattct gtaaggtgta caccataacc ccactgctca gcgacaaccg ggagaagcgg     3240

ggtctcgccc tggatgggaa actcaagcac gaggacacca acctggcttc cagcaccatc     3300

gtgaaggagg gtgccaacaa ggaggtgctg ggaatcctgg tgtcctacag ggtcaaggtg     3360

aagctggtgg tgtctcgagg cggggatgtc tctgtggagc tgccttttgt tcttatgcac     3420

cccaagcccc acgaccacat ccccctcccc agaccccagt cagccgctcc ggagacagat     3480

gtccctgtgg acaccaacct cattgaattt gataccaact atgccacaga tgatgacatt     3540

gtgtttgagg actttgcccg gcttcggctg aaggggatga aggatgacga ctatgatgat     3600

caactctgct cgaagaagac ttaccccaac gacaaaacca tcatcagtac ctttaagtgg     3660

agttacacca ctggaaatgg caagcgctac cggagcactg cgcggaccac ctacaccttt     3720

gccaagccaa tggcggctaa ctatctgaag aaccagccga tgtacgtgtt ccgtaagacg     3780

gagctcaagc actccaagac cgagctcaac ttcaaggagt ggcaaaaggc ctttaccgat     3840

gtgatgggca tggacgagct gtacaagtag cgcgccctcg actgtgcctt ctagttgcca     3900

gccatctgta tcgcggccgc tctagaccag gcgcctggat ccagatcact tctggctaat     3960

aaaagatcag agctctagag atctgtgtgt tggttttttg tggatctgct gtgccttcta     4020

gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca     4080

ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc     4140

attctattct ggggggtggg gtggggcagc acagcaaggg ggaggattgg gaagacaata     4200

gcaggcatgc tggggatgcg gtgggctcta tgggtacctc tctctctctc tctctctctc     4260

tctctctctc tctctctcgg tacctctctc tctctctctc tctctctctc tctctctctc     4320

tctcggtacc aggtgctgaa gaattgaccc ggttcctcct gggccagaaa gaagcaggca     4380

catccccttc tctgtgacac accctgtcca cgcccctggt tcttagttcc agccccactc     4440

ataggacact catagctcag gagggctccg ccttcaatcc cacccgctaa agtacttgga     4500

gcggtctctc cctccctcat cagcccacca aaccaaacct agcctccaag agtgggaaga     4560

aattaaagca agataggcta ttaagtgcag agggagagaa aatgcctcca acatgtgagg     4620

aagtaatgag agaaatcata gaatttcttc cgcttcctcg ctcactgact cgctgcgctc     4680

ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac     4740

agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa     4800

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca     4860

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc     4920

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata     4980

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta     5040

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca     5100

gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga     5160

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg     5220

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg     5280

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg     5340

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     5400

aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa     5460

cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat     5520

ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc     5580

tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc     5640

atccatagtt gcctgactcc gggggggggg ggcgctgagg tctgcctcgt gaagaaggtg     5700

ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac     5760

ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca     5820

cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc     5880

gatttattca acaaagccgc cgtcccgtca agtcagcgta atgctctgcc agtgttacaa     5940

ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt     6000

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa     6060

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg     6120

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa     6180

atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca     6240

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc     6300

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca     6360

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     6420

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     6480

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     6540

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     6600

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     6660

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     6720

gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc     6780

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc     6840

ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttcccccc ccccccatta     6900

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa     6960

aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga     7020

aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc      7079


<210>  23
<211>  7079
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 4A coding sequence


<220>
<221>  misc_feature
<222>  (1)..(450)
<223>  mNeonGreen

<220>
<221>  misc_feature
<222>  (451)..(1674)
<223>  B-Arrestin

<220>
<221>  misc_feature
<222>  (451)..(453)
<223>  Met to Gly mutation

<220>
<221>  misc_feature
<222>  (1675)..(1935)
<223>  mNeonGreen

<400>  23
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg ggtgaaatac cgcacagatg cgtaaggaga aaataccgca tcagattggc      240

tattggccat tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt      300

ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg      360

gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc      420

ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc      480

atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact      540

gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat      600

gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact      660

tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac      720

atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac      780

gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac      840

tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga      900

gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat      960

agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt     1020

ccccgtgcca agagtgacgt aagtaccgcc tatagactct ataggcacac ccctttggct     1080

cttatgcatg ctatactgtt tttggcttgg ggcctataca cccccgcttc cttatgctat     1140

aggtgatggt atagcttagc ctataggtgt gggttattga ccattattga ccactcccct     1200

attggtgacg atactttcca ttactaatcc ataacatggc tctttgccac aactatctct     1260

attggctata tgccaatact ctgtccttca gagactgaca cggactctgt atttttacag     1320

gatggggtcc catttattat ttacaaattc acatatacaa caacgccgtc ccccgtgccc     1380

gcagttttta ttaaacatag cgtgggatct ccacgcgaat ctcgggtacg tgttccggac     1440

atgggctctt ctccggtagc ggcggagctt ccacatccga gccctggtcc catgcctcca     1500

gcggctcatg gtcgctcggc agctccttgc tcctaacagt ggaggccaga cttaggcaca     1560

gcacaatgcc caccaccacc agtgtgccgc acaaggccgt ggcggtaggg tatgtgtctg     1620

aaaatgagcg tggagattgg gctcgcacgg ctgacgcaga tggaagactt aaggcagcgg     1680

cagaagaaga tgcaggcagc tgagttgttg tattctgata agagtcagag gtaactcccg     1740

ttgcggtgct gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc     1800

gcgccaccag acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct     1860

gcagtcaccg tcgtcgacac gtgtgatcag atatacgact cactataggg agacccaagc     1920

tggctagcgt ttaccatggt gagcaagggc gaggaggata acatggcctc tctcccagcg     1980

acacatgagt tacacatctt tggctccatc aacggtgtgg actttgacat ggtgggtcag     2040

ggcaccggca atccaaatga tggttatgag gagttaaacc tgaagtccac caagggtgac     2100

ctccagttct ccccctggat tctggtccct catatcgggt atggcttcca tcagtacctg     2160

ccctaccctg acgggatgtc gcctttccag gccgccatgg tagatggctc cggataccaa     2220

gtccatcgca caatgcagtt tgaagatggt gcctccctta ctgttaacta ccgctacacc     2280

tacgagggaa gccacatcaa aggagaggcc caggtgaagg ggactggttt ccctgctgac     2340

ggtcctgtga tgaccaactc gctgaccgct gcggactggt gcagggggga gaaacccggg     2400

accagggtct tcaagaagtc gagccctaac tgcaagctca ccgtgtactt gggcaagcgg     2460

gacttcgtag atcacctgga caaagtggac cctgtagatg gcgtggtgct tgtggaccct     2520

gactacctga aggaccgcaa agtgtttgtg accctcacct gcgccttccg ctatggccgt     2580

gaagacctgg atgtgctggg cttgtccttc cgcaaagacc tgttcatcgc cacctaccag     2640

gccttccccc cggtgcccaa cccaccccgg ccccccaccc gcctgcagga ccggctgctg     2700

aggaagctgg gccagcatgc ccaccccttc ttcttcacca taccccagaa tcttccatgc     2760

tccgtcacac tgcagccagg cccagaggat acaggaaagg cctgcggcgt agactttgag     2820

attcgagcct tctgtgctaa atcactagaa gagaaaagcc acaaaaggaa ctctgtgcgg     2880

ctggtgatcc gaaaggtgca gttcgccccg gagaaacccg gcccccagcc ttcagccgaa     2940

accacacgcc acttcctcat gtctgaccgg tccctgcacc tcgaggcttc cctggacaag     3000

gagctgtact accatgggga gcccctcaat gtaaatgtcc acgtcaccaa caactccacc     3060

aagaccgtca agaagatcaa agtctctgtg agacagtacg ccgacatctg cctcttcagc     3120

accgcccagt acaagtgtcc tgtggctcaa ctcgaacaag atgaccaggt atctcccagc     3180

tccacattct gtaaggtgta caccataacc ccactgctca gcgacaaccg ggagaagcgg     3240

ggtctcgccc tggatgggaa actcaagcac gaggacacca acctggcttc cagcaccatc     3300

gtgaaggagg gtgccaacaa ggaggtgctg ggaatcctgg tgtcctacag ggtcaaggtg     3360

aagctggtgg tgtctcgagg cggggatgtc tctgtggagc tgccttttgt tcttatgcac     3420

cccaagcccc acgaccacat ccccctcccc agaccccagt cagccgctcc ggagacagat     3480

gtccctgtgg acaccaacct cattgaattt gataccaact atgccacaga tgatgacatt     3540

gtgtttgagg actttgcccg gcttcggctg aaggggatga aggatgacga ctatgatgat     3600

caactctgct cgaagaagac ttaccccaac gacaaaacca tcatcagtac ctttaagtgg     3660

agttacacca ctggaaatgg caagcgctac cggagcactg cgcggaccac ctacaccttt     3720

gccaagccaa tggcggctaa ctatctgaag aaccagccga tgtacgtgtt ccgtaagacg     3780

gagctcaagc actccaagac cgagctcaac ttcaaggagt ggcaaaaggc ctttaccgat     3840

gtgatgggca tggacgagct gtacaagtag cgcgccctcg actgtgcctt ctagttgcca     3900

gccatctgta tcgcggccgc tctagaccag gcgcctggat ccagatcact tctggctaat     3960

aaaagatcag agctctagag atctgtgtgt tggttttttg tggatctgct gtgccttcta     4020

gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca     4080

ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc     4140

attctattct ggggggtggg gtggggcagc acagcaaggg ggaggattgg gaagacaata     4200

gcaggcatgc tggggatgcg gtgggctcta tgggtacctc tctctctctc tctctctctc     4260

tctctctctc tctctctcgg tacctctctc tctctctctc tctctctctc tctctctctc     4320

tctcggtacc aggtgctgaa gaattgaccc ggttcctcct gggccagaaa gaagcaggca     4380

catccccttc tctgtgacac accctgtcca cgcccctggt tcttagttcc agccccactc     4440

ataggacact catagctcag gagggctccg ccttcaatcc cacccgctaa agtacttgga     4500

gcggtctctc cctccctcat cagcccacca aaccaaacct agcctccaag agtgggaaga     4560

aattaaagca agataggcta ttaagtgcag agggagagaa aatgcctcca acatgtgagg     4620

aagtaatgag agaaatcata gaatttcttc cgcttcctcg ctcactgact cgctgcgctc     4680

ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac     4740

agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa     4800

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca     4860

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc     4920

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata     4980

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta     5040

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca     5100

gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga     5160

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg     5220

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg     5280

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg     5340

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     5400

aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa     5460

cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat     5520

ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc     5580

tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc     5640

atccatagtt gcctgactcc gggggggggg ggcgctgagg tctgcctcgt gaagaaggtg     5700

ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac     5760

ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca     5820

cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc     5880

gatttattca acaaagccgc cgtcccgtca agtcagcgta atgctctgcc agtgttacaa     5940

ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt     6000

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa     6060

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg     6120

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa     6180

atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca     6240

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc     6300

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca     6360

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     6420

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     6480

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     6540

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     6600

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     6660

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     6720

gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc     6780

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc     6840

ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttcccccc ccccccatta     6900

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa     6960

aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga     7020

aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc      7079


<210>  24
<211>  644
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 4A coding sequence


<220>
<221>  SITE
<222>  (1)..(150)
<223>  mNeonGreen

<220>
<221>  SITE
<222>  (151)..(558)
<223>  B-arrestin

<220>
<221>  SITE
<222>  (151)..(151)
<223>  Met to Gly mutation

<220>
<221>  SITE
<222>  (559)..(644)
<223>  mNeonGreen

<400>  24

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr 
1               5                   10                  15      


His Glu Leu His Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met 
            20                  25                  30          


Val Gly Gln Gly Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn 
        35                  40                  45              


Leu Lys Ser Thr Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val 
    50                  55                  60                  


Pro His Ile Gly Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly 
65                  70                  75                  80  


Met Ser Pro Phe Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val 
                85                  90                  95      


His Arg Thr Met Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr 
            100                 105                 110         


Arg Tyr Thr Tyr Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys 
        115                 120                 125             


Gly Thr Gly Phe Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr 
    130                 135                 140                 


Ala Ala Asp Trp Cys Arg Gly Glu Lys Pro Gly Thr Arg Val Phe Lys 
145                 150                 155                 160 


Lys Ser Ser Pro Asn Cys Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp 
                165                 170                 175     


Phe Val Asp His Leu Asp Lys Val Asp Pro Val Asp Gly Val Val Leu 
            180                 185                 190         


Val Asp Pro Asp Tyr Leu Lys Asp Arg Lys Val Phe Val Thr Leu Thr 
        195                 200                 205             


Cys Ala Phe Arg Tyr Gly Arg Glu Asp Leu Asp Val Leu Gly Leu Ser 
    210                 215                 220                 


Phe Arg Lys Asp Leu Phe Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val 
225                 230                 235                 240 


Pro Asn Pro Pro Arg Pro Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg 
                245                 250                 255     


Lys Leu Gly Gln His Ala His Pro Phe Phe Phe Thr Ile Pro Gln Asn 
            260                 265                 270         


Leu Pro Cys Ser Val Thr Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys 
        275                 280                 285             


Ala Cys Gly Val Asp Phe Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu 
    290                 295                 300                 


Glu Glu Lys Ser His Lys Arg Asn Ser Val Arg Leu Val Ile Arg Lys 
305                 310                 315                 320 


Val Gln Phe Ala Pro Glu Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr 
                325                 330                 335     


Thr Arg His Phe Leu Met Ser Asp Arg Ser Leu His Leu Glu Ala Ser 
            340                 345                 350         


Leu Asp Lys Glu Leu Tyr Tyr His Gly Glu Pro Leu Asn Val Asn Val 
        355                 360                 365             


His Val Thr Asn Asn Ser Thr Lys Thr Val Lys Lys Ile Lys Val Ser 
    370                 375                 380                 


Val Arg Gln Tyr Ala Asp Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys 
385                 390                 395                 400 


Cys Pro Val Ala Gln Leu Glu Gln Asp Asp Gln Val Ser Pro Ser Ser 
                405                 410                 415     


Thr Phe Cys Lys Val Tyr Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg 
            420                 425                 430         


Glu Lys Arg Gly Leu Ala Leu Asp Gly Lys Leu Lys His Glu Asp Thr 
        435                 440                 445             


Asn Leu Ala Ser Ser Thr Ile Val Lys Glu Gly Ala Asn Lys Glu Val 
    450                 455                 460                 


Leu Gly Ile Leu Val Ser Tyr Arg Val Lys Val Lys Leu Val Val Ser 
465                 470                 475                 480 


Arg Gly Gly Asp Val Ser Val Glu Leu Pro Phe Val Leu Met His Pro 
                485                 490                 495     


Lys Pro His Asp His Ile Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro 
            500                 505                 510         


Glu Thr Asp Val Pro Val Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn 
        515                 520                 525             


Tyr Ala Thr Asp Asp Asp Ile Val Phe Glu Asp Phe Ala Arg Leu Arg 
    530                 535                 540                 


Leu Lys Gly Met Lys Asp Asp Asp Tyr Asp Asp Gln Leu Cys Ser Lys 
545                 550                 555                 560 


Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys Trp Ser 
                565                 570                 575     


Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg Thr Thr 
            580                 585                 590         


Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn Gln Pro 
        595                 600                 605             


Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr Glu Leu 
    610                 615                 620                 


Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly Met Asp 
625                 630                 635                 640 


Glu Leu Tyr Lys 
                


<210>  25
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 4A mNeonGreen Part 1


<220>
<221>  misc_feature
<222>  (1)..(450)
<223>  mNeonGreen

<220>
<221>  misc_feature
<222>  (256)..(265)
<223>  Kozak sequence

<400>  25
atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca tgagttacac       60

atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac cggcaatcca      120

aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc      180

tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta ccctgacggg      240

atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca tcgcacaatg      300

cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga gggaagccac      360

atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc      420

aactcgctga ccgctgcgga ctggtgcagg                                       450


<210>  26
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 4A - mNeonGreen Part 1


<220>
<221>  SITE
<222>  (1)..(150)
<223>  mNeonGreen Part 1

<400>  26

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr 
1               5                   10                  15      


His Glu Leu His Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met 
            20                  25                  30          


Val Gly Gln Gly Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn 
        35                  40                  45              


Leu Lys Ser Thr Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val 
    50                  55                  60                  


Pro His Ile Gly Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly 
65                  70                  75                  80  


Met Ser Pro Phe Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val 
                85                  90                  95      


His Arg Thr Met Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr 
            100                 105                 110         


Arg Tyr Thr Tyr Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys 
        115                 120                 125             


Gly Thr Gly Phe Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr 
    130                 135                 140                 


Ala Ala Asp Trp Cys Arg 
145                 150 


<210>  27
<211>  1224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 4A Beta-arrestin-2


<220>
<221>  misc_feature
<222>  (1)..(1224)
<223>  B-arrestin

<400>  27
ggggagaaac ccgggaccag ggtcttcaag aagtcgagcc ctaactgcaa gctcaccgtg       60

tacttgggca agcgggactt cgtagatcac ctggacaaag tggaccctgt agatggcgtg      120

gtgcttgtgg accctgacta cctgaaggac cgcaaagtgt ttgtgaccct cacctgcgcc      180

ttccgctatg gccgtgaaga cctggatgtg ctgggcttgt ccttccgcaa agacctgttc      240

atcgccacct accaggcctt ccccccggtg cccaacccac cccggccccc cacccgcctg      300

caggaccggc tgctgaggaa gctgggccag catgcccacc ccttcttctt caccataccc      360

cagaatcttc catgctccgt cacactgcag ccaggcccag aggatacagg aaaggcctgc      420

ggcgtagact ttgagattcg agccttctgt gctaaatcac tagaagagaa aagccacaaa      480

aggaactctg tgcggctggt gatccgaaag gtgcagttcg ccccggagaa acccggcccc      540

cagccttcag ccgaaaccac acgccacttc ctcatgtctg accggtccct gcacctcgag      600

gcttccctgg acaaggagct gtactaccat ggggagcccc tcaatgtaaa tgtccacgtc      660

accaacaact ccaccaagac cgtcaagaag atcaaagtct ctgtgagaca gtacgccgac      720

atctgcctct tcagcaccgc ccagtacaag tgtcctgtgg ctcaactcga acaagatgac      780

caggtatctc ccagctccac attctgtaag gtgtacacca taaccccact gctcagcgac      840

aaccgggaga agcggggtct cgccctggat gggaaactca agcacgagga caccaacctg      900

gcttccagca ccatcgtgaa ggagggtgcc aacaaggagg tgctgggaat cctggtgtcc      960

tacagggtca aggtgaagct ggtggtgtct cgaggcgggg atgtctctgt ggagctgcct     1020

tttgttctta tgcaccccaa gccccacgac cacatccccc tccccagacc ccagtcagcc     1080

gctccggaga cagatgtccc tgtggacacc aacctcattg aatttgatac caactatgcc     1140

acagatgatg acattgtgtt tgaggacttt gcccggcttc ggctgaaggg gatgaaggat     1200

gacgactatg atgatcaact ctgc                                            1224


<210>  28
<211>  408
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 4A Beta-arrestin-2


<220>
<221>  SITE
<222>  (1)..(408)
<223>  B-arrestin

<400>  28

Gly Glu Lys Pro Gly Thr Arg Val Phe Lys Lys Ser Ser Pro Asn Cys 
1               5                   10                  15      


Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp His Leu Asp 
            20                  25                  30          


Lys Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro Asp Tyr Leu 
        35                  40                  45              


Lys Asp Arg Lys Val Phe Val Thr Leu Thr Cys Ala Phe Arg Tyr Gly 
    50                  55                  60                  


Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys Asp Leu Phe 
65                  70                  75                  80  


Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val Pro Asn Pro Pro Arg Pro 
                85                  90                  95      


Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg Lys Leu Gly Gln His Ala 
            100                 105                 110         


His Pro Phe Phe Phe Thr Ile Pro Gln Asn Leu Pro Cys Ser Val Thr 
        115                 120                 125             


Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp Phe 
    130                 135                 140                 


Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu Glu Glu Lys Ser His Lys 
145                 150                 155                 160 


Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Phe Ala Pro Glu 
                165                 170                 175     


Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr Thr Arg His Phe Leu Met 
            180                 185                 190         


Ser Asp Arg Ser Leu His Leu Glu Ala Ser Leu Asp Lys Glu Leu Tyr 
        195                 200                 205             


Tyr His Gly Glu Pro Leu Asn Val Asn Val His Val Thr Asn Asn Ser 
    210                 215                 220                 


Thr Lys Thr Val Lys Lys Ile Lys Val Ser Val Arg Gln Tyr Ala Asp 
225                 230                 235                 240 


Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys Cys Pro Val Ala Gln Leu 
                245                 250                 255     


Glu Gln Asp Asp Gln Val Ser Pro Ser Ser Thr Phe Cys Lys Val Tyr 
            260                 265                 270         


Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg Glu Lys Arg Gly Leu Ala 
        275                 280                 285             


Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser Thr 
    290                 295                 300                 


Ile Val Lys Glu Gly Ala Asn Lys Glu Val Leu Gly Ile Leu Val Ser 
305                 310                 315                 320 


Tyr Arg Val Lys Val Lys Leu Val Val Ser Arg Gly Gly Asp Val Ser 
                325                 330                 335     


Val Glu Leu Pro Phe Val Leu Met His Pro Lys Pro His Asp His Ile 
            340                 345                 350         


Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro Glu Thr Asp Val Pro Val 
        355                 360                 365             


Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn Tyr Ala Thr Asp Asp Asp 
    370                 375                 380                 


Ile Val Phe Glu Asp Phe Ala Arg Leu Arg Leu Lys Gly Met Lys Asp 
385                 390                 395                 400 


Asp Asp Tyr Asp Asp Gln Leu Cys 
                405             


<210>  29
<211>  261
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 4A - mNeonGreen Part 2


<220>
<221>  misc_feature
<222>  (1)..(261)
<223>  mNeonGreen Part 2

<400>  29
tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc       60

actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca      120

atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag      180

cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc      240

atggacgagc tgtacaagta g                                                261


<210>  30
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 4A - mNeonGreen Part 2


<220>
<221>  SITE
<222>  (1)..(86)
<223>  mNeonGreen Part 2

<400>  30

Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys 
1               5                   10                  15      


Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg 
            20                  25                  30          


Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn 
        35                  40                  45              


Gln Pro Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr 
    50                  55                  60                  


Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly 
65                  70                  75                  80  


Met Asp Glu Leu Tyr Lys 
                85      


<210>  31
<211>  7094
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 5A - plasmid


<220>
<221>  misc_feature
<222>  (320)..(699)
<223>  CMV enhancer

<220>
<221>  misc_feature
<222>  (700)..(903)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (1039)..(1864)
<223>  CMV Intron

<220>
<221>  misc_feature
<222>  (1936)..(2385)
<223>  mNeonGreen

<220>
<221>  misc_feature
<222>  (2386)..(2394)
<223>  N-terminal linker

<220>
<221>  misc_feature
<222>  (2386)..(2397)
<223>  N-terminal linker

<220>
<221>  misc_feature
<222>  (2395)..(3618)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (3619)..(3624)
<223>  C-terminal linker

<220>
<221>  misc_feature
<222>  (3625)..(3885)
<223>  mNeonGreen

<220>
<221>  misc_feature
<222>  (4024)..(4248)
<223>  bGH poly(A) signal

<220>
<221>  misc_feature
<222>  (4843)..(5431)
<223>  ori

<220>
<221>  misc_feature
<222>  (5974)..(6789)
<223>  KanR

<400>  31
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg ggtgaaatac cgcacagatg cgtaaggaga aaataccgca tcagattggc      240

tattggccat tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt      300

ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg      360

gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc      420

ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc      480

atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact      540

gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat      600

gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact      660

tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac      720

atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac      780

gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac      840

tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga      900

gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat      960

agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt     1020

ccccgtgcca agagtgacgt aagtaccgcc tatagactct ataggcacac ccctttggct     1080

cttatgcatg ctatactgtt tttggcttgg ggcctataca cccccgcttc cttatgctat     1140

aggtgatggt atagcttagc ctataggtgt gggttattga ccattattga ccactcccct     1200

attggtgacg atactttcca ttactaatcc ataacatggc tctttgccac aactatctct     1260

attggctata tgccaatact ctgtccttca gagactgaca cggactctgt atttttacag     1320

gatggggtcc catttattat ttacaaattc acatatacaa caacgccgtc ccccgtgccc     1380

gcagttttta ttaaacatag cgtgggatct ccacgcgaat ctcgggtacg tgttccggac     1440

atgggctctt ctccggtagc ggcggagctt ccacatccga gccctggtcc catgcctcca     1500

gcggctcatg gtcgctcggc agctccttgc tcctaacagt ggaggccaga cttaggcaca     1560

gcacaatgcc caccaccacc agtgtgccgc acaaggccgt ggcggtaggg tatgtgtctg     1620

aaaatgagcg tggagattgg gctcgcacgg ctgacgcaga tggaagactt aaggcagcgg     1680

cagaagaaga tgcaggcagc tgagttgttg tattctgata agagtcagag gtaactcccg     1740

ttgcggtgct gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc     1800

gcgccaccag acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct     1860

gcagtcaccg tcgtcgacac gtgtgatcag atatacgact cactataggg agacccaagc     1920

tggctagcgt ttaccatggt gagcaagggc gaggaggata acatggcctc tctcccagcg     1980

acacatgagt tacacatctt tggctccatc aacggtgtgg actttgacat ggtgggtcag     2040

ggcaccggca atccaaatga tggttatgag gagttaaacc tgaagtccac caagggtgac     2100

ctccagttct ccccctggat tctggtccct catatcgggt atggcttcca tcagtacctg     2160

ccctaccctg acgggatgtc gcctttccag gccgccatgg tagatggctc cggataccaa     2220

gtccatcgca caatgcagtt tgaagatggt gcctccctta ctgttaacta ccgctacacc     2280

tacgagggaa gccacatcaa aggagaggcc caggtgaagg ggactggttt ccctgctgac     2340

ggtcctgtga tgaccaactc gctgaccgct gcggactggt gcaggatgcg cggaggggag     2400

aaacccggga ccagggtctt caagaagtcg agccctaact gcaagctcac cgtgtacttg     2460

ggcaagcggg acttcgtaga tcacctggac aaagtggacc ctgtagatgg cgtggtgctt     2520

gtggaccctg actacctgaa ggaccgcaaa gtgtttgtga ccctcacctg cgccttccgc     2580

tatggccgtg aagacctgga tgtgctgggc ttgtccttcc gcaaagacct gttcatcgcc     2640

acctaccagg ccttcccccc ggtgcccaac ccaccccggc cccccacccg cctgcaggac     2700

cggctgctga ggaagctggg ccagcatgcc caccccttct tcttcaccat accccagaat     2760

cttccatgct ccgtcacact gcagccaggc ccagaggata caggaaaggc ctgcggcgta     2820

gactttgaga ttcgagcctt ctgtgctaaa tcactagaag agaaaagcca caaaaggaac     2880

tctgtgcggc tggtgatccg aaaggtgcag ttcgccccgg agaaacccgg cccccagcct     2940

tcagccgaaa ccacacgcca cttcctcatg tctgaccggt ccctgcacct cgaggcttcc     3000

ctggacaagg agctgtacta ccatggggag cccctcaatg taaatgtcca cgtcaccaac     3060

aactccacca agaccgtcaa gaagatcaaa gtctctgtga gacagtacgc cgacatctgc     3120

ctcttcagca ccgcccagta caagtgtcct gtggctcaac tcgaacaaga tgaccaggta     3180

tctcccagct ccacattctg taaggtgtac accataaccc cactgctcag cgacaaccgg     3240

gagaagcggg gtctcgccct ggatgggaaa ctcaagcacg aggacaccaa cctggcttcc     3300

agcaccatcg tgaaggaggg tgccaacaag gaggtgctgg gaatcctggt gtcctacagg     3360

gtcaaggtga agctggtggt gtctcgaggc ggggatgtct ctgtggagct gccttttgtt     3420

cttatgcacc ccaagcccca cgaccacatc cccctcccca gaccccagtc agccgctccg     3480

gagacagatg tccctgtgga caccaacctc attgaatttg ataccaacta tgccacagat     3540

gatgacattg tgtttgagga ctttgcccgg cttcggctga aggggatgaa ggatgacgac     3600

tatgatgatc aactctgcaa tgtttcgaag aagacttacc ccaacgacaa aaccatcatc     3660

agtaccttta agtggagtta caccactgga aatggcaagc gctaccggag cactgcgcgg     3720

accacctaca cctttgccaa gccaatggcg gctaactatc tgaagaacca gccgatgtac     3780

gtgttccgta agacggagct caagcactcc aagaccgagc tcaacttcaa ggagtggcaa     3840

aaggccttta ccgatgtgat gggcatggac gagctgtaca agtagcgcgc cctcgactgt     3900

gccttctagt tgccagccat ctgtatcgcg gccgctctag accaggcgcc tggatccaga     3960

tcacttctgg ctaataaaag atcagagctc tagagatctg tgtgttggtt ttttgtggat     4020

ctgctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga     4080

ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt     4140

gtctgagtag gtgtcattct attctggggg gtggggtggg gcagcacagc aagggggagg     4200

attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt acctctctct     4260

ctctctctct ctctctctct ctctctctct ctcggtacct ctctctctct ctctctctct     4320

ctctctctct ctctctctcg gtaccaggtg ctgaagaatt gacccggttc ctcctgggcc     4380

agaaagaagc aggcacatcc ccttctctgt gacacaccct gtccacgccc ctggttctta     4440

gttccagccc cactcatagg acactcatag ctcaggaggg ctccgccttc aatcccaccc     4500

gctaaagtac ttggagcggt ctctccctcc ctcatcagcc caccaaacca aacctagcct     4560

ccaagagtgg gaagaaatta aagcaagata ggctattaag tgcagaggga gagaaaatgc     4620

ctccaacatg tgaggaagta atgagagaaa tcatagaatt tcttccgctt cctcgctcac     4680

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     4740

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     4800

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     4860

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     4920

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     4980

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg     5040

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     5100

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     5160

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     5220

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     5280

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     5340

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     5400

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     5460

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     5520

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     5580

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     5640

ctgtctattt cgttcatcca tagttgcctg actccggggg gggggggcgc tgaggtctgc     5700

ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga     5760

aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga     5820

acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca     5880

actcagcaaa agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct     5940

ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg     6000

aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg     6060

taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc     6120

tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag     6180

gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt     6240

atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact     6300

cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc     6360

gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag     6420

cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt     6480

cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat     6540

ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc     6600

attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata     6660

caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata     6720

taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat     6780

atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga     6840

tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttc     6900

cccccccccc cattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt     6960

tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc     7020

acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac     7080

gaggcccttt cgtc                                                       7094


<210>  32
<211>  1950
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 5A - coding sequence


<220>
<221>  misc_feature
<222>  (1)..(450)
<223>  mNeonGreen Part 1

<220>
<221>  misc_feature
<222>  (451)..(459)
<223>  N-terminal linker

<220>
<221>  misc_feature
<222>  (451)..(462)
<223>  N-terminal linker

<220>
<221>  misc_feature
<222>  (460)..(1683)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (1684)..(1689)
<223>  C-terminal linker

<220>
<221>  misc_feature
<222>  (1690)..(1950)
<223>  mNeonGreen Part 2

<400>  32
atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca tgagttacac       60

atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac cggcaatcca      120

aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc      180

tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta ccctgacggg      240

atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca tcgcacaatg      300

cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga gggaagccac      360

atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc      420

aactcgctga ccgctgcgga ctggtgcagg atgcgcggag gggagaaacc cgggaccagg      480

gtcttcaaga agtcgagccc taactgcaag ctcaccgtgt acttgggcaa gcgggacttc      540

gtagatcacc tggacaaagt ggaccctgta gatggcgtgg tgcttgtgga ccctgactac      600

ctgaaggacc gcaaagtgtt tgtgaccctc acctgcgcct tccgctatgg ccgtgaagac      660

ctggatgtgc tgggcttgtc cttccgcaaa gacctgttca tcgccaccta ccaggccttc      720

cccccggtgc ccaacccacc ccggcccccc acccgcctgc aggaccggct gctgaggaag      780

ctgggccagc atgcccaccc cttcttcttc accatacccc agaatcttcc atgctccgtc      840

acactgcagc caggcccaga ggatacagga aaggcctgcg gcgtagactt tgagattcga      900

gccttctgtg ctaaatcact agaagagaaa agccacaaaa ggaactctgt gcggctggtg      960

atccgaaagg tgcagttcgc cccggagaaa cccggccccc agccttcagc cgaaaccaca     1020

cgccacttcc tcatgtctga ccggtccctg cacctcgagg cttccctgga caaggagctg     1080

tactaccatg gggagcccct caatgtaaat gtccacgtca ccaacaactc caccaagacc     1140

gtcaagaaga tcaaagtctc tgtgagacag tacgccgaca tctgcctctt cagcaccgcc     1200

cagtacaagt gtcctgtggc tcaactcgaa caagatgacc aggtatctcc cagctccaca     1260

ttctgtaagg tgtacaccat aaccccactg ctcagcgaca accgggagaa gcggggtctc     1320

gccctggatg ggaaactcaa gcacgaggac accaacctgg cttccagcac catcgtgaag     1380

gagggtgcca acaaggaggt gctgggaatc ctggtgtcct acagggtcaa ggtgaagctg     1440

gtggtgtctc gaggcgggga tgtctctgtg gagctgcctt ttgttcttat gcaccccaag     1500

ccccacgacc acatccccct ccccagaccc cagtcagccg ctccggagac agatgtccct     1560

gtggacacca acctcattga atttgatacc aactatgcca cagatgatga cattgtgttt     1620

gaggactttg cccggcttcg gctgaagggg atgaaggatg acgactatga tgatcaactc     1680

tgcaatgttt cgaagaagac ttaccccaac gacaaaacca tcatcagtac ctttaagtgg     1740

agttacacca ctggaaatgg caagcgctac cggagcactg cgcggaccac ctacaccttt     1800

gccaagccaa tggcggctaa ctatctgaag aaccagccga tgtacgtgtt ccgtaagacg     1860

gagctcaagc actccaagac cgagctcaac ttcaaggagt ggcaaaaggc ctttaccgat     1920

gtgatgggca tggacgagct gtacaagtag                                      1950


<210>  33
<211>  649
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 5A - coding sequence


<220>
<221>  SITE
<222>  (1)..(150)
<223>  mNeonGreen Part 1

<220>
<221>  SITE
<222>  (151)..(153)
<223>  N-terminal linker

<220>
<221>  SITE
<222>  (151)..(154)
<223>  N-terminal linker

<220>
<221>  SITE
<222>  (155)..(561)
<223>  B-arrestin

<220>
<221>  SITE
<222>  (562)..(563)
<223>  C-terminal linker

<220>
<221>  SITE
<222>  (564)..(649)
<223>  mNeonGreen Part 2

<400>  33

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr 
1               5                   10                  15      


His Glu Leu His Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met 
            20                  25                  30          


Val Gly Gln Gly Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn 
        35                  40                  45              


Leu Lys Ser Thr Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val 
    50                  55                  60                  


Pro His Ile Gly Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly 
65                  70                  75                  80  


Met Ser Pro Phe Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val 
                85                  90                  95      


His Arg Thr Met Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr 
            100                 105                 110         


Arg Tyr Thr Tyr Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys 
        115                 120                 125             


Gly Thr Gly Phe Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr 
    130                 135                 140                 


Ala Ala Asp Trp Cys Arg Met Arg Gly Gly Glu Lys Pro Gly Thr Arg 
145                 150                 155                 160 


Val Phe Lys Lys Ser Ser Pro Asn Cys Lys Leu Thr Val Tyr Leu Gly 
                165                 170                 175     


Lys Arg Asp Phe Val Asp His Leu Asp Lys Val Asp Pro Val Asp Gly 
            180                 185                 190         


Val Val Leu Val Asp Pro Asp Tyr Leu Lys Asp Arg Lys Val Phe Val 
        195                 200                 205             


Thr Leu Thr Cys Ala Phe Arg Tyr Gly Arg Glu Asp Leu Asp Val Leu 
    210                 215                 220                 


Gly Leu Ser Phe Arg Lys Asp Leu Phe Ile Ala Thr Tyr Gln Ala Phe 
225                 230                 235                 240 


Pro Pro Val Pro Asn Pro Pro Arg Pro Pro Thr Arg Leu Gln Asp Arg 
                245                 250                 255     


Leu Leu Arg Lys Leu Gly Gln His Ala His Pro Phe Phe Phe Thr Ile 
            260                 265                 270         


Pro Gln Asn Leu Pro Cys Ser Val Thr Leu Gln Pro Gly Pro Glu Asp 
        275                 280                 285             


Thr Gly Lys Ala Cys Gly Val Asp Phe Glu Ile Arg Ala Phe Cys Ala 
    290                 295                 300                 


Lys Ser Leu Glu Glu Lys Ser His Lys Arg Asn Ser Val Arg Leu Val 
305                 310                 315                 320 


Ile Arg Lys Val Gln Phe Ala Pro Glu Lys Pro Gly Pro Gln Pro Ser 
                325                 330                 335     


Ala Glu Thr Thr Arg His Phe Leu Met Ser Asp Arg Ser Leu His Leu 
            340                 345                 350         


Glu Ala Ser Leu Asp Lys Glu Leu Tyr Tyr His Gly Glu Pro Leu Asn 
        355                 360                 365             


Val Asn Val His Val Thr Asn Asn Ser Thr Lys Thr Val Lys Lys Ile 
    370                 375                 380                 


Lys Val Ser Val Arg Gln Tyr Ala Asp Ile Cys Leu Phe Ser Thr Ala 
385                 390                 395                 400 


Gln Tyr Lys Cys Pro Val Ala Gln Leu Glu Gln Asp Asp Gln Val Ser 
                405                 410                 415     


Pro Ser Ser Thr Phe Cys Lys Val Tyr Thr Ile Thr Pro Leu Leu Ser 
            420                 425                 430         


Asp Asn Arg Glu Lys Arg Gly Leu Ala Leu Asp Gly Lys Leu Lys His 
        435                 440                 445             


Glu Asp Thr Asn Leu Ala Ser Ser Thr Ile Val Lys Glu Gly Ala Asn 
    450                 455                 460                 


Lys Glu Val Leu Gly Ile Leu Val Ser Tyr Arg Val Lys Val Lys Leu 
465                 470                 475                 480 


Val Val Ser Arg Gly Gly Asp Val Ser Val Glu Leu Pro Phe Val Leu 
                485                 490                 495     


Met His Pro Lys Pro His Asp His Ile Pro Leu Pro Arg Pro Gln Ser 
            500                 505                 510         


Ala Ala Pro Glu Thr Asp Val Pro Val Asp Thr Asn Leu Ile Glu Phe 
        515                 520                 525             


Asp Thr Asn Tyr Ala Thr Asp Asp Asp Ile Val Phe Glu Asp Phe Ala 
    530                 535                 540                 


Arg Leu Arg Leu Lys Gly Met Lys Asp Asp Asp Tyr Asp Asp Gln Leu 
545                 550                 555                 560 


Cys Asn Val Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser 
                565                 570                 575     


Thr Phe Lys Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser 
            580                 585                 590         


Thr Ala Arg Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr 
        595                 600                 605             


Leu Lys Asn Gln Pro Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His 
    610                 615                 620                 


Ser Lys Thr Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp 
625                 630                 635                 640 


Val Met Gly Met Asp Glu Leu Tyr Lys 
                645                 


<210>  34
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 5A - mNeonGreen Part 1


<220>
<221>  misc_feature
<222>  (1)..(450)
<223>  mNeonGreen Part 1

<400>  34
atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca tgagttacac       60

atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac cggcaatcca      120

aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc      180

tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta ccctgacggg      240

atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca tcgcacaatg      300

cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga gggaagccac      360

atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc      420

aactcgctga ccgctgcgga ctggtgcagg                                       450


<210>  35
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 5A - mNeonGreen Part 1


<220>
<221>  SITE
<222>  (1)..(150)
<223>  mNeonGreen Part 1

<400>  35

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr 
1               5                   10                  15      


His Glu Leu His Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met 
            20                  25                  30          


Val Gly Gln Gly Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn 
        35                  40                  45              


Leu Lys Ser Thr Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val 
    50                  55                  60                  


Pro His Ile Gly Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly 
65                  70                  75                  80  


Met Ser Pro Phe Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val 
                85                  90                  95      


His Arg Thr Met Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr 
            100                 105                 110         


Arg Tyr Thr Tyr Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys 
        115                 120                 125             


Gly Thr Gly Phe Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr 
    130                 135                 140                 


Ala Ala Asp Trp Cys Arg 
145                 150 


<210>  36
<211>  1224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 5A - Beta-arrestin-2


<220>
<221>  SITE
<222>  (1)..(1224)
<223>  B-arrestin

<400>  36
ggggagaaac ccgggaccag ggtcttcaag aagtcgagcc ctaactgcaa gctcaccgtg       60

tacttgggca agcgggactt cgtagatcac ctggacaaag tggaccctgt agatggcgtg      120

gtgcttgtgg accctgacta cctgaaggac cgcaaagtgt ttgtgaccct cacctgcgcc      180

ttccgctatg gccgtgaaga cctggatgtg ctgggcttgt ccttccgcaa agacctgttc      240

atcgccacct accaggcctt ccccccggtg cccaacccac cccggccccc cacccgcctg      300

caggaccggc tgctgaggaa gctgggccag catgcccacc ccttcttctt caccataccc      360

cagaatcttc catgctccgt cacactgcag ccaggcccag aggatacagg aaaggcctgc      420

ggcgtagact ttgagattcg agccttctgt gctaaatcac tagaagagaa aagccacaaa      480

aggaactctg tgcggctggt gatccgaaag gtgcagttcg ccccggagaa acccggcccc      540

cagccttcag ccgaaaccac acgccacttc ctcatgtctg accggtccct gcacctcgag      600

gcttccctgg acaaggagct gtactaccat ggggagcccc tcaatgtaaa tgtccacgtc      660

accaacaact ccaccaagac cgtcaagaag atcaaagtct ctgtgagaca gtacgccgac      720

atctgcctct tcagcaccgc ccagtacaag tgtcctgtgg ctcaactcga acaagatgac      780

caggtatctc ccagctccac attctgtaag gtgtacacca taaccccact gctcagcgac      840

aaccgggaga agcggggtct cgccctggat gggaaactca agcacgagga caccaacctg      900

gcttccagca ccatcgtgaa ggagggtgcc aacaaggagg tgctgggaat cctggtgtcc      960

tacagggtca aggtgaagct ggtggtgtct cgaggcgggg atgtctctgt ggagctgcct     1020

tttgttctta tgcaccccaa gccccacgac cacatccccc tccccagacc ccagtcagcc     1080

gctccggaga cagatgtccc tgtggacacc aacctcattg aatttgatac caactatgcc     1140

acagatgatg acattgtgtt tgaggacttt gcccggcttc ggctgaaggg gatgaaggat     1200

gacgactatg atgatcaact ctgc                                            1224


<210>  37
<211>  408
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 5A - Beta-arrestin-2


<220>
<221>  SITE
<222>  (1)..(408)
<223>  B-arrestin

<400>  37

Gly Glu Lys Pro Gly Thr Arg Val Phe Lys Lys Ser Ser Pro Asn Cys 
1               5                   10                  15      


Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp His Leu Asp 
            20                  25                  30          


Lys Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro Asp Tyr Leu 
        35                  40                  45              


Lys Asp Arg Lys Val Phe Val Thr Leu Thr Cys Ala Phe Arg Tyr Gly 
    50                  55                  60                  


Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys Asp Leu Phe 
65                  70                  75                  80  


Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val Pro Asn Pro Pro Arg Pro 
                85                  90                  95      


Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg Lys Leu Gly Gln His Ala 
            100                 105                 110         


His Pro Phe Phe Phe Thr Ile Pro Gln Asn Leu Pro Cys Ser Val Thr 
        115                 120                 125             


Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp Phe 
    130                 135                 140                 


Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu Glu Glu Lys Ser His Lys 
145                 150                 155                 160 


Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Phe Ala Pro Glu 
                165                 170                 175     


Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr Thr Arg His Phe Leu Met 
            180                 185                 190         


Ser Asp Arg Ser Leu His Leu Glu Ala Ser Leu Asp Lys Glu Leu Tyr 
        195                 200                 205             


Tyr His Gly Glu Pro Leu Asn Val Asn Val His Val Thr Asn Asn Ser 
    210                 215                 220                 


Thr Lys Thr Val Lys Lys Ile Lys Val Ser Val Arg Gln Tyr Ala Asp 
225                 230                 235                 240 


Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys Cys Pro Val Ala Gln Leu 
                245                 250                 255     


Glu Gln Asp Asp Gln Val Ser Pro Ser Ser Thr Phe Cys Lys Val Tyr 
            260                 265                 270         


Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg Glu Lys Arg Gly Leu Ala 
        275                 280                 285             


Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser Thr 
    290                 295                 300                 


Ile Val Lys Glu Gly Ala Asn Lys Glu Val Leu Gly Ile Leu Val Ser 
305                 310                 315                 320 


Tyr Arg Val Lys Val Lys Leu Val Val Ser Arg Gly Gly Asp Val Ser 
                325                 330                 335     


Val Glu Leu Pro Phe Val Leu Met His Pro Lys Pro His Asp His Ile 
            340                 345                 350         


Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro Glu Thr Asp Val Pro Val 
        355                 360                 365             


Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn Tyr Ala Thr Asp Asp Asp 
    370                 375                 380                 


Ile Val Phe Glu Asp Phe Ala Arg Leu Arg Leu Lys Gly Met Lys Asp 
385                 390                 395                 400 


Asp Asp Tyr Asp Asp Gln Leu Cys 
                405             


<210>  38
<211>  261
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 5A - mNeonGreen Part 2


<220>
<221>  SITE
<222>  (1)..(261)
<223>  mNeonGreen Part 2

<400>  38
tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc       60

actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca      120

atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag      180

cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc      240

atggacgagc tgtacaagta g                                                261


<210>  39
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 5A - mNeonGreen Part 2


<220>
<221>  SITE
<222>  (1)..(86)
<223>  mNeonGreen Part 2

<400>  39

Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys 
1               5                   10                  15      


Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg 
            20                  25                  30          


Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn 
        35                  40                  45              


Gln Pro Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr 
    50                  55                  60                  


Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly 
65                  70                  75                  80  


Met Asp Glu Leu Tyr Lys 
                85      


<210>  40
<211>  7085
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 6B - plasmid


<220>
<221>  misc_feature
<222>  (320)..(699)
<223>  CMV enhancer

<220>
<221>  misc_feature
<222>  (700)..(903)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (1039)..(1864)
<223>  CMV Intron

<220>
<221>  misc_feature
<222>  (1936)..(2376)
<223>  mNeonGreen Part 1

<220>
<221>  misc_feature
<222>  (2377)..(2388)
<223>  N-terminal linker

<220>
<221>  misc_feature
<222>  (2386)..(3609)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (3610)..(3615)
<223>  C-terminal linker

<220>
<221>  misc_feature
<222>  (3616)..(3876)
<223>  mNeonGreen Part 2

<400>  40
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg ggtgaaatac cgcacagatg cgtaaggaga aaataccgca tcagattggc      240

tattggccat tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt      300

ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg      360

gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc      420

ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc      480

atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact      540

gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat      600

gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact      660

tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac      720

atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac      780

gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac      840

tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga      900

gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat      960

agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt     1020

ccccgtgcca agagtgacgt aagtaccgcc tatagactct ataggcacac ccctttggct     1080

cttatgcatg ctatactgtt tttggcttgg ggcctataca cccccgcttc cttatgctat     1140

aggtgatggt atagcttagc ctataggtgt gggttattga ccattattga ccactcccct     1200

attggtgacg atactttcca ttactaatcc ataacatggc tctttgccac aactatctct     1260

attggctata tgccaatact ctgtccttca gagactgaca cggactctgt atttttacag     1320

gatggggtcc catttattat ttacaaattc acatatacaa caacgccgtc ccccgtgccc     1380

gcagttttta ttaaacatag cgtgggatct ccacgcgaat ctcgggtacg tgttccggac     1440

atgggctctt ctccggtagc ggcggagctt ccacatccga gccctggtcc catgcctcca     1500

gcggctcatg gtcgctcggc agctccttgc tcctaacagt ggaggccaga cttaggcaca     1560

gcacaatgcc caccaccacc agtgtgccgc acaaggccgt ggcggtaggg tatgtgtctg     1620

aaaatgagcg tggagattgg gctcgcacgg ctgacgcaga tggaagactt aaggcagcgg     1680

cagaagaaga tgcaggcagc tgagttgttg tattctgata agagtcagag gtaactcccg     1740

ttgcggtgct gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc     1800

gcgccaccag acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct     1860

gcagtcaccg tcgtcgacac gtgtgatcag atatacgact cactataggg agacccaagc     1920

tggctagcgt ttaccatggt gagcaagggc gaggaggata acatggcctc tctcccagcg     1980

acacatgagt tacacatctt tggctccatc aacggtgtgg actttgacat ggtgggtcag     2040

ggcaccggca atccaaatga tggttatgag gagttaaacc tgaagtccac caagggtgac     2100

ctccagttct ccccctggat tctggtccct catatcgggt atggcttcca tcagtacctg     2160

ccctaccctg acgggatgtc gcctttccag gccgccatgg tagatggctc cggataccaa     2220

gtccatcgca caatgcagtt tgaagatggt gcctccctta ctgttaacta ccgctacacc     2280

tacgagggaa gccacatcaa aggagaggcc caggtgaagg ggactggttt ccctgctgac     2340

ggtcctgtga tgaccaactc gctgaccgct gcggactgcg acatcgggga gaaacccggg     2400

accagggtct tcaagaagtc gagccctaac tgcaagctca ccgtgtactt gggcaagcgg     2460

gacttcgtag atcacctgga caaagtggac cctgtagatg gcgtggtgct tgtggaccct     2520

gactacctga aggaccgcaa agtgtttgtg accctcacct gcgccttccg ctatggccgt     2580

gaagacctgg atgtgctggg cttgtccttc cgcaaagacc tgttcatcgc cacctaccag     2640

gccttccccc cggtgcccaa cccaccccgg ccccccaccc gcctgcagga ccggctgctg     2700

aggaagctgg gccagcatgc ccaccccttc ttcttcacca taccccagaa tcttccatgc     2760

tccgtcacac tgcagccagg cccagaggat acaggaaagg cctgcggcgt agactttgag     2820

attcgagcct tctgtgctaa atcactagaa gagaaaagcc acaaaaggaa ctctgtgcgg     2880

ctggtgatcc gaaaggtgca gttcgccccg gagaaacccg gcccccagcc ttcagccgaa     2940

accacacgcc acttcctcat gtctgaccgg tccctgcacc tcgaggcttc cctggacaag     3000

gagctgtact accatgggga gcccctcaat gtaaatgtcc acgtcaccaa caactccacc     3060

aagaccgtca agaagatcaa agtctctgtg agacagtacg ccgacatctg cctcttcagc     3120

accgcccagt acaagtgtcc tgtggctcaa ctcgaacaag atgaccaggt atctcccagc     3180

tccacattct gtaaggtgta caccataacc ccactgctca gcgacaaccg ggagaagcgg     3240

ggtctcgccc tggatgggaa actcaagcac gaggacacca acctggcttc cagcaccatc     3300

gtgaaggagg gtgccaacaa ggaggtgctg ggaatcctgg tgtcctacag ggtcaaggtg     3360

aagctggtgg tgtctcgagg cggggatgtc tctgtggagc tgccttttgt tcttatgcac     3420

cccaagcccc acgaccacat ccccctcccc agaccccagt cagccgctcc ggagacagat     3480

gtccctgtgg acaccaacct cattgaattt gataccaact atgccacaga tgatgacatt     3540

gtgtttgagg actttgcccg gcttcggctg aaggggatga aggatgacga ctatgatgat     3600

caactctgca atgtttcgaa gaagacttac cccaacgaca aaaccatcat cagtaccttt     3660

aagtggagtt acaccactgg aaatggcaag cgctaccgga gcactgcgcg gaccacctac     3720

acctttgcca agccaatggc ggctaactat ctgaagaacc agccgatgta cgtgttccgt     3780

aagacggagc tcaagcactc caagaccgag ctcaacttca aggagtggca aaaggccttt     3840

accgatgtga tgggcatgga cgagctgtac aagtagcgcg ccctcgactg tgccttctag     3900

ttgccagcca tctgtatcgc ggccgctcta gaccaggcgc ctggatccag atcacttctg     3960

gctaataaaa gatcagagct ctagagatct gtgtgttggt tttttgtgga tctgctgtgc     4020

cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag     4080

gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta     4140

ggtgtcattc tattctgggg ggtggggtgg ggcagcacag caagggggag gattgggaag     4200

acaatagcag gcatgctggg gatgcggtgg gctctatggg tacctctctc tctctctctc     4260

tctctctctc tctctctctc tctcggtacc tctctctctc tctctctctc tctctctctc     4320

tctctctctc ggtaccaggt gctgaagaat tgacccggtt cctcctgggc cagaaagaag     4380

caggcacatc cccttctctg tgacacaccc tgtccacgcc cctggttctt agttccagcc     4440

ccactcatag gacactcata gctcaggagg gctccgcctt caatcccacc cgctaaagta     4500

cttggagcgg tctctccctc cctcatcagc ccaccaaacc aaacctagcc tccaagagtg     4560

ggaagaaatt aaagcaagat aggctattaa gtgcagaggg agagaaaatg cctccaacat     4620

gtgaggaagt aatgagagaa atcatagaat ttcttccgct tcctcgctca ctgactcgct     4680

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     4740

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     4800

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     4860

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     4920

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     4980

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg     5040

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     5100

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     5160

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     5220

aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt     5280

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     5340

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     5400

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     5460

gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac     5520

ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac     5580

ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt     5640

tcgttcatcc atagttgcct gactccgggg ggggggggcg ctgaggtctg cctcgtgaag     5700

aaggtgttgc tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg     5760

agccacggtt gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct     5820

ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa     5880

aagttcgatt tattcaacaa agccgccgtc ccgtcaagtc agcgtaatgc tctgccagtg     5940

ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa     6000

tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg     6060

agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc     6120

gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag     6180

tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc     6240

tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac     6300

caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa     6360

aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac     6420

aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat     6480

cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag     6540

aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac     6600

gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata     6660

gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc     6720

atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat     6780

aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt     6840

tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt cccccccccc     6900

ccattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat     6960

ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt     7020

ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt     7080

tcgtc                                                                 7085


<210>  41
<211>  1941
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 6B - coding sequence


<220>
<221>  misc_feature
<222>  (1)..(441)
<223>  mNeonGreen Part 1

<220>
<221>  misc_feature
<222>  (442)..(453)
<223>  N-terminal linker

<220>
<221>  misc_feature
<222>  (451)..(1674)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (1675)..(1680)
<223>  C-Terminal linker

<220>
<221>  misc_feature
<222>  (1681)..(1941)
<223>  mNeonGreen Part 2

<400>  41
atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca tgagttacac       60

atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac cggcaatcca      120

aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc      180

tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta ccctgacggg      240

atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca tcgcacaatg      300

cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga gggaagccac      360

atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc      420

aactcgctga ccgctgcgga ctgcgacatc ggggagaaac ccgggaccag ggtcttcaag      480

aagtcgagcc ctaactgcaa gctcaccgtg tacttgggca agcgggactt cgtagatcac      540

ctggacaaag tggaccctgt agatggcgtg gtgcttgtgg accctgacta cctgaaggac      600

cgcaaagtgt ttgtgaccct cacctgcgcc ttccgctatg gccgtgaaga cctggatgtg      660

ctgggcttgt ccttccgcaa agacctgttc atcgccacct accaggcctt ccccccggtg      720

cccaacccac cccggccccc cacccgcctg caggaccggc tgctgaggaa gctgggccag      780

catgcccacc ccttcttctt caccataccc cagaatcttc catgctccgt cacactgcag      840

ccaggcccag aggatacagg aaaggcctgc ggcgtagact ttgagattcg agccttctgt      900

gctaaatcac tagaagagaa aagccacaaa aggaactctg tgcggctggt gatccgaaag      960

gtgcagttcg ccccggagaa acccggcccc cagccttcag ccgaaaccac acgccacttc     1020

ctcatgtctg accggtccct gcacctcgag gcttccctgg acaaggagct gtactaccat     1080

ggggagcccc tcaatgtaaa tgtccacgtc accaacaact ccaccaagac cgtcaagaag     1140

atcaaagtct ctgtgagaca gtacgccgac atctgcctct tcagcaccgc ccagtacaag     1200

tgtcctgtgg ctcaactcga acaagatgac caggtatctc ccagctccac attctgtaag     1260

gtgtacacca taaccccact gctcagcgac aaccgggaga agcggggtct cgccctggat     1320

gggaaactca agcacgagga caccaacctg gcttccagca ccatcgtgaa ggagggtgcc     1380

aacaaggagg tgctgggaat cctggtgtcc tacagggtca aggtgaagct ggtggtgtct     1440

cgaggcgggg atgtctctgt ggagctgcct tttgttctta tgcaccccaa gccccacgac     1500

cacatccccc tccccagacc ccagtcagcc gctccggaga cagatgtccc tgtggacacc     1560

aacctcattg aatttgatac caactatgcc acagatgatg acattgtgtt tgaggacttt     1620

gcccggcttc ggctgaaggg gatgaaggat gacgactatg atgatcaact ctgcaatgtt     1680

tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc     1740

actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca     1800

atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag     1860

cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc     1920

atggacgagc tgtacaagta g                                               1941


<210>  42
<211>  646
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 6B - coding sequence


<220>
<221>  SITE
<222>  (1)..(147)
<223>  mNeonGreen Part 1

<220>
<221>  SITE
<222>  (148)..(151)
<223>  N-terminal linker

<220>
<221>  SITE
<222>  (151)..(558)
<223>  B-arrestin

<220>
<221>  SITE
<222>  (559)..(560)
<223>  C-terminal linker

<220>
<221>  SITE
<222>  (561)..(646)
<223>  mNeonGreen Part 2

<400>  42

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr 
1               5                   10                  15      


His Glu Leu His Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met 
            20                  25                  30          


Val Gly Gln Gly Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn 
        35                  40                  45              


Leu Lys Ser Thr Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val 
    50                  55                  60                  


Pro His Ile Gly Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly 
65                  70                  75                  80  


Met Ser Pro Phe Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val 
                85                  90                  95      


His Arg Thr Met Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr 
            100                 105                 110         


Arg Tyr Thr Tyr Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys 
        115                 120                 125             


Gly Thr Gly Phe Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr 
    130                 135                 140                 


Ala Ala Asp Cys Asp Ile Gly Glu Lys Pro Gly Thr Arg Val Phe Lys 
145                 150                 155                 160 


Lys Ser Ser Pro Asn Cys Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp 
                165                 170                 175     


Phe Val Asp His Leu Asp Lys Val Asp Pro Val Asp Gly Val Val Leu 
            180                 185                 190         


Val Asp Pro Asp Tyr Leu Lys Asp Arg Lys Val Phe Val Thr Leu Thr 
        195                 200                 205             


Cys Ala Phe Arg Tyr Gly Arg Glu Asp Leu Asp Val Leu Gly Leu Ser 
    210                 215                 220                 


Phe Arg Lys Asp Leu Phe Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val 
225                 230                 235                 240 


Pro Asn Pro Pro Arg Pro Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg 
                245                 250                 255     


Lys Leu Gly Gln His Ala His Pro Phe Phe Phe Thr Ile Pro Gln Asn 
            260                 265                 270         


Leu Pro Cys Ser Val Thr Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys 
        275                 280                 285             


Ala Cys Gly Val Asp Phe Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu 
    290                 295                 300                 


Glu Glu Lys Ser His Lys Arg Asn Ser Val Arg Leu Val Ile Arg Lys 
305                 310                 315                 320 


Val Gln Phe Ala Pro Glu Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr 
                325                 330                 335     


Thr Arg His Phe Leu Met Ser Asp Arg Ser Leu His Leu Glu Ala Ser 
            340                 345                 350         


Leu Asp Lys Glu Leu Tyr Tyr His Gly Glu Pro Leu Asn Val Asn Val 
        355                 360                 365             


His Val Thr Asn Asn Ser Thr Lys Thr Val Lys Lys Ile Lys Val Ser 
    370                 375                 380                 


Val Arg Gln Tyr Ala Asp Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys 
385                 390                 395                 400 


Cys Pro Val Ala Gln Leu Glu Gln Asp Asp Gln Val Ser Pro Ser Ser 
                405                 410                 415     


Thr Phe Cys Lys Val Tyr Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg 
            420                 425                 430         


Glu Lys Arg Gly Leu Ala Leu Asp Gly Lys Leu Lys His Glu Asp Thr 
        435                 440                 445             


Asn Leu Ala Ser Ser Thr Ile Val Lys Glu Gly Ala Asn Lys Glu Val 
    450                 455                 460                 


Leu Gly Ile Leu Val Ser Tyr Arg Val Lys Val Lys Leu Val Val Ser 
465                 470                 475                 480 


Arg Gly Gly Asp Val Ser Val Glu Leu Pro Phe Val Leu Met His Pro 
                485                 490                 495     


Lys Pro His Asp His Ile Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro 
            500                 505                 510         


Glu Thr Asp Val Pro Val Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn 
        515                 520                 525             


Tyr Ala Thr Asp Asp Asp Ile Val Phe Glu Asp Phe Ala Arg Leu Arg 
    530                 535                 540                 


Leu Lys Gly Met Lys Asp Asp Asp Tyr Asp Asp Gln Leu Cys Asn Val 
545                 550                 555                 560 


Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys 
                565                 570                 575     


Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg 
            580                 585                 590         


Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn 
        595                 600                 605             


Gln Pro Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr 
    610                 615                 620                 


Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly 
625                 630                 635                 640 


Met Asp Glu Leu Tyr Lys 
                645     


<210>  43
<211>  441
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 6B - mNeonGreen Part 1


<220>
<221>  misc_feature
<222>  (1)..(441)
<223>  mNeonGreen Part 1

<400>  43
atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca tgagttacac       60

atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac cggcaatcca      120

aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc      180

tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta ccctgacggg      240

atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca tcgcacaatg      300

cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga gggaagccac      360

atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc      420

aactcgctga ccgctgcgga c                                                441


<210>  44
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 6B - mNeonGreen Part 1


<220>
<221>  SITE
<222>  (1)..(147)
<223>  mNeonGreen Part 1

<400>  44

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr 
1               5                   10                  15      


His Glu Leu His Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met 
            20                  25                  30          


Val Gly Gln Gly Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn 
        35                  40                  45              


Leu Lys Ser Thr Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val 
    50                  55                  60                  


Pro His Ile Gly Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly 
65                  70                  75                  80  


Met Ser Pro Phe Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val 
                85                  90                  95      


His Arg Thr Met Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr 
            100                 105                 110         


Arg Tyr Thr Tyr Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys 
        115                 120                 125             


Gly Thr Gly Phe Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr 
    130                 135                 140                 


Ala Ala Asp 
145         


<210>  45
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 6B - 1st linker sequence


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  N-Terminal linker

<400>  45
tgcgacatcg gg                                                           12


<210>  46
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 6b - 1st Linker Sequence

<400>  46

Cys Asp Ile Gly 
1               


<210>  47
<211>  1224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 6B - Beta-arrestin-2


<220>
<221>  misc_feature
<222>  (1)..(1224)
<223>  B-arrestin

<400>  47
ggggagaaac ccgggaccag ggtcttcaag aagtcgagcc ctaactgcaa gctcaccgtg       60

tacttgggca agcgggactt cgtagatcac ctggacaaag tggaccctgt agatggcgtg      120

gtgcttgtgg accctgacta cctgaaggac cgcaaagtgt ttgtgaccct cacctgcgcc      180

ttccgctatg gccgtgaaga cctggatgtg ctgggcttgt ccttccgcaa agacctgttc      240

atcgccacct accaggcctt ccccccggtg cccaacccac cccggccccc cacccgcctg      300

caggaccggc tgctgaggaa gctgggccag catgcccacc ccttcttctt caccataccc      360

cagaatcttc catgctccgt cacactgcag ccaggcccag aggatacagg aaaggcctgc      420

ggcgtagact ttgagattcg agccttctgt gctaaatcac tagaagagaa aagccacaaa      480

aggaactctg tgcggctggt gatccgaaag gtgcagttcg ccccggagaa acccggcccc      540

cagccttcag ccgaaaccac acgccacttc ctcatgtctg accggtccct gcacctcgag      600

gcttccctgg acaaggagct gtactaccat ggggagcccc tcaatgtaaa tgtccacgtc      660

accaacaact ccaccaagac cgtcaagaag atcaaagtct ctgtgagaca gtacgccgac      720

atctgcctct tcagcaccgc ccagtacaag tgtcctgtgg ctcaactcga acaagatgac      780

caggtatctc ccagctccac attctgtaag gtgtacacca taaccccact gctcagcgac      840

aaccgggaga agcggggtct cgccctggat gggaaactca agcacgagga caccaacctg      900

gcttccagca ccatcgtgaa ggagggtgcc aacaaggagg tgctgggaat cctggtgtcc      960

tacagggtca aggtgaagct ggtggtgtct cgaggcgggg atgtctctgt ggagctgcct     1020

tttgttctta tgcaccccaa gccccacgac cacatccccc tccccagacc ccagtcagcc     1080

gctccggaga cagatgtccc tgtggacacc aacctcattg aatttgatac caactatgcc     1140

acagatgatg acattgtgtt tgaggacttt gcccggcttc ggctgaaggg gatgaaggat     1200

gacgactatg atgatcaact ctgc                                            1224


<210>  48
<211>  408
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 6B - Beta-arrestin-2


<220>
<221>  SITE
<222>  (1)..(408)
<223>  B-arrestin

<400>  48

Gly Glu Lys Pro Gly Thr Arg Val Phe Lys Lys Ser Ser Pro Asn Cys 
1               5                   10                  15      


Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp His Leu Asp 
            20                  25                  30          


Lys Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro Asp Tyr Leu 
        35                  40                  45              


Lys Asp Arg Lys Val Phe Val Thr Leu Thr Cys Ala Phe Arg Tyr Gly 
    50                  55                  60                  


Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys Asp Leu Phe 
65                  70                  75                  80  


Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val Pro Asn Pro Pro Arg Pro 
                85                  90                  95      


Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg Lys Leu Gly Gln His Ala 
            100                 105                 110         


His Pro Phe Phe Phe Thr Ile Pro Gln Asn Leu Pro Cys Ser Val Thr 
        115                 120                 125             


Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp Phe 
    130                 135                 140                 


Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu Glu Glu Lys Ser His Lys 
145                 150                 155                 160 


Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Phe Ala Pro Glu 
                165                 170                 175     


Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr Thr Arg His Phe Leu Met 
            180                 185                 190         


Ser Asp Arg Ser Leu His Leu Glu Ala Ser Leu Asp Lys Glu Leu Tyr 
        195                 200                 205             


Tyr His Gly Glu Pro Leu Asn Val Asn Val His Val Thr Asn Asn Ser 
    210                 215                 220                 


Thr Lys Thr Val Lys Lys Ile Lys Val Ser Val Arg Gln Tyr Ala Asp 
225                 230                 235                 240 


Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys Cys Pro Val Ala Gln Leu 
                245                 250                 255     


Glu Gln Asp Asp Gln Val Ser Pro Ser Ser Thr Phe Cys Lys Val Tyr 
            260                 265                 270         


Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg Glu Lys Arg Gly Leu Ala 
        275                 280                 285             


Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser Thr 
    290                 295                 300                 


Ile Val Lys Glu Gly Ala Asn Lys Glu Val Leu Gly Ile Leu Val Ser 
305                 310                 315                 320 


Tyr Arg Val Lys Val Lys Leu Val Val Ser Arg Gly Gly Asp Val Ser 
                325                 330                 335     


Val Glu Leu Pro Phe Val Leu Met His Pro Lys Pro His Asp His Ile 
            340                 345                 350         


Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro Glu Thr Asp Val Pro Val 
        355                 360                 365             


Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn Tyr Ala Thr Asp Asp Asp 
    370                 375                 380                 


Ile Val Phe Glu Asp Phe Ala Arg Leu Arg Leu Lys Gly Met Lys Asp 
385                 390                 395                 400 


Asp Asp Tyr Asp Asp Gln Leu Cys 
                405             


<210>  49
<211>  261
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr 6B - mNeonGreen Part 2


<220>
<221>  misc_feature
<222>  (1)..(261)
<223>  mNeonGreen Part 2

<400>  49
tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc       60

actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca      120

atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag      180

cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc      240

atggacgagc tgtacaagta g                                                261


<210>  50
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr 6B - mNeonGreen Part 2


<220>
<221>  SITE
<222>  (1)..(86)
<223>  mNeonGreen Part 2

<400>  50

Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys 
1               5                   10                  15      


Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg 
            20                  25                  30          


Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn 
        35                  40                  45              


Gln Pro Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr 
    50                  55                  60                  


Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly 
65                  70                  75                  80  


Met Asp Glu Leu Tyr Lys 
                85      


<210>  51
<211>  7094
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr L1-3B8 - plasmid


<220>
<221>  misc_feature
<222>  (320)..(699)
<223>  CMV enhancer

<220>
<221>  misc_feature
<222>  (700)..(903)
<223>  CMV promoter

<220>
<221>  misc_feature
<222>  (1039)..(1864)
<223>  CMV Intron A

<220>
<221>  misc_feature
<222>  (1936)..(2385)
<223>  mNeonGreen

<220>
<221>  misc_feature
<222>  (1936)..(3885)
<223>  arrestin sensor BArrL1-3

<220>
<221>  misc_feature
<222>  (2386)..(2397)
<223>  N-terminal linker

<220>
<221>  misc_feature
<222>  (2395)..(3618)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (3619)..(3624)
<223>  C-terminal linker

<220>
<221>  misc_feature
<222>  (3625)..(3885)
<223>  mNeonGreen Part 2

<220>
<221>  misc_feature
<222>  (3886)..(3900)
<223>  pUB/pKM1 joint

<220>
<221>  misc_feature
<222>  (3906)..(4248)
<223>  PolyA Terminator

<220>
<221>  misc_feature
<222>  (3906)..(4248)
<223>  bGH poly(A) signal

<220>
<221>  misc_feature
<222>  (4843)..(5431)
<223>  ori

<220>
<221>  misc_feature
<222>  (5974)..(6789)
<223>  KanR

<400>  51
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg ggtgaaatac cgcacagatg cgtaaggaga aaataccgca tcagattggc      240

tattggccat tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt      300

ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg      360

gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc      420

ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc      480

atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact      540

gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat      600

gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact      660

tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac      720

atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac      780

gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac      840

tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga      900

gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat      960

agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt     1020

ccccgtgcca agagtgacgt aagtaccgcc tatagactct ataggcacac ccctttggct     1080

cttatgcatg ctatactgtt tttggcttgg ggcctataca cccccgcttc cttatgctat     1140

aggtgatggt atagcttagc ctataggtgt gggttattga ccattattga ccactcccct     1200

attggtgacg atactttcca ttactaatcc ataacatggc tctttgccac aactatctct     1260

attggctata tgccaatact ctgtccttca gagactgaca cggactctgt atttttacag     1320

gatggggtcc catttattat ttacaaattc acatatacaa caacgccgtc ccccgtgccc     1380

gcagttttta ttaaacatag cgtgggatct ccacgcgaat ctcgggtacg tgttccggac     1440

atgggctctt ctccggtagc ggcggagctt ccacatccga gccctggtcc catgcctcca     1500

gcggctcatg gtcgctcggc agctccttgc tcctaacagt ggaggccaga cttaggcaca     1560

gcacaatgcc caccaccacc agtgtgccgc acaaggccgt ggcggtaggg tatgtgtctg     1620

aaaatgagcg tggagattgg gctcgcacgg ctgacgcaga tggaagactt aaggcagcgg     1680

cagaagaaga tgcaggcagc tgagttgttg tattctgata agagtcagag gtaactcccg     1740

ttgcggtgct gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc     1800

gcgccaccag acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct     1860

gcagtcaccg tcgtcgacac gtgtgatcag atatacgact cactataggg agacccaagc     1920

tggctagcgt ttaccatggt gagcaagggc gaggaggata acatggcctc tctcccagcg     1980

acacatgagt tacacatctt tggctccatc aacggtgtgg actttgacat ggtgggtcag     2040

ggcaccggca atccaaatga tggttatgag gagttaaacc tgaagtccac caagggtgac     2100

ctccagttct ccccctggat tctggtccct catatcgggt atggcttcca tcagtacctg     2160

ccctaccctg acgggatgtc gcctttccag gccgccatgg tagatggctc cggataccaa     2220

gtccatcgca caatgcagtt tgaagatggt gcctccctta ctgttaacta ccgctacacc     2280

tacgagggaa gccacatcaa aggagaggcc caggtgaagg ggactggttt ccctgctgac     2340

ggtcctgtga tgaccaactc gctgaccgct gcggactggt gcaggatcct ttggggggag     2400

aaacccggga ccagggtctt caagaagtcg agccctaact gcaagctcac cgtgtacttg     2460

ggcaagcggg acttcgtaga tcacctggac aaagtggacc ctgtagatgg cgtggtgctt     2520

gtggaccctg actacctgaa ggaccgcaaa gtgtttgtga ccctcacctg cgccttccgc     2580

tatggccgtg aagacctgga tgtgctgggc ttgtccttcc gcaaagacct gttcatcgcc     2640

acctaccagg ccttcccccc ggtgcccaac ccaccccggc cccccacccg cctgcaggac     2700

cggctgctga ggaagctggg ccagcatgcc caccccttct tcttcaccat accccagaat     2760

cttccatgct ccgtcacact gcagccaggc ccagaggata caggaaaggc ctgcggcgta     2820

gactttgaga ttcgagcctt ctgtgctaaa tcactagaag agaaaagcca caaaaggaac     2880

tctgtgcggc tggtgatccg aaaggtgcag ttcgccccgg agaaacccgg cccccagcct     2940

tcagccgaaa ccacacgcca cttcctcatg tctgaccggt ccctgcacct cgaggcttcc     3000

ctggacaagg agctgtacta ccatggggag cccctcaatg taaatgtcca cgtcaccaac     3060

aactccacca agaccgtcaa gaagatcaaa gtctctgtga gacagtacgc cgacatctgc     3120

ctcttcagca ccgcccagta caagtgtcct gtggctcaac tcgaacaaga tgaccaggta     3180

tctcccagct ccacattctg taaggtgtac accataaccc cactgctcag cgacaaccgg     3240

gagaagcggg gtctcgccct ggatgggaaa ctcaagcacg aggacaccaa cctggcttcc     3300

agcaccatcg tgaaggaggg tgccaacaag gaggtgctgg gaatcctggt gtcctacagg     3360

gtcaaggtga agctggtggt gtctcgaggc ggggatgtct ctgtggagct gccttttgtt     3420

cttatgcacc ccaagcccca cgaccacatc cccctcccca gaccccagtc agccgctccg     3480

gagacagatg tccctgtgga caccaacctc attgaatttg ataccaacta tgccacagat     3540

gatgacattg tgtttgagga ctttgcccgg cttcggctga aggggatgaa ggatgacgac     3600

tatgatgatc aactctgcaa tgtttcgaag aagacttacc ccaacgacaa aaccatcatc     3660

agtaccttta agtggagtta caccactgga aatggcaagc gctaccggag cactgcgcgg     3720

accacctaca cctttgccaa gccaatggcg gctaactatc tgaagaacca gccgatgtac     3780

gtgttccgta agacggagct caagcactcc aagaccgagc tcaacttcaa ggagtggcaa     3840

aaggccttta ccgatgtgat gggcatggac gagctgtaca agtagcgcgc cctcgactgt     3900

gccttctagt tgccagccat ctgtatcgcg gccgctctag accaggcgcc tggatccaga     3960

tcacttctgg ctaataaaag atcagagctc tagagatctg tgtgttggtt ttttgtggat     4020

ctgctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga     4080

ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt     4140

gtctgagtag gtgtcattct attctggggg gtggggtggg gcagcacagc aagggggagg     4200

attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt acctctctct     4260

ctctctctct ctctctctct ctctctctct ctcggtacct ctctctctct ctctctctct     4320

ctctctctct ctctctctcg gtaccaggtg ctgaagaatt gacccggttc ctcctgggcc     4380

agaaagaagc aggcacatcc ccttctctgt gacacaccct gtccacgccc ctggttctta     4440

gttccagccc cactcatagg acactcatag ctcaggaggg ctccgccttc aatcccaccc     4500

gctaaagtac ttggagcggt ctctccctcc ctcatcagcc caccaaacca aacctagcct     4560

ccaagagtgg gaagaaatta aagcaagata ggctattaag tgcagaggga gagaaaatgc     4620

ctccaacatg tgaggaagta atgagagaaa tcatagaatt tcttccgctt cctcgctcac     4680

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     4740

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     4800

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     4860

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     4920

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     4980

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg     5040

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     5100

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     5160

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     5220

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     5280

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     5340

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     5400

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     5460

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     5520

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     5580

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     5640

ctgtctattt cgttcatcca tagttgcctg actccggggg gggggggcgc tgaggtctgc     5700

ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga     5760

aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga     5820

acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca     5880

actcagcaaa agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct     5940

ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg     6000

aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg     6060

taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc     6120

tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag     6180

gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt     6240

atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact     6300

cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc     6360

gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag     6420

cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt     6480

cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat     6540

ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc     6600

attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata     6660

caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata     6720

taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat     6780

atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga     6840

tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttc     6900

cccccccccc cattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt     6960

tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc     7020

acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac     7080

gaggcccttt cgtc                                                       7094


<210>  52
<211>  1950
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr L1-3B8 - coding sequence


<220>
<221>  misc_feature
<222>  (1)..(450)
<223>  mNeonGreen Part 1

<220>
<221>  misc_feature
<222>  (451)..(462)
<223>  N-terminal linker

<220>
<221>  misc_feature
<222>  (460)..(1683)
<223>  B-arrestin

<220>
<221>  misc_feature
<222>  (1684)..(1689)
<223>  C-Terminal linker

<220>
<221>  misc_feature
<222>  (1690)..(1950)
<223>  mNeonGreen Part 2

<400>  52
atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca tgagttacac       60

atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac cggcaatcca      120

aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc      180

tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta ccctgacggg      240

atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca tcgcacaatg      300

cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga gggaagccac      360

atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc      420

aactcgctga ccgctgcgga ctggtgcagg atcctttggg gggagaaacc cgggaccagg      480

gtcttcaaga agtcgagccc taactgcaag ctcaccgtgt acttgggcaa gcgggacttc      540

gtagatcacc tggacaaagt ggaccctgta gatggcgtgg tgcttgtgga ccctgactac      600

ctgaaggacc gcaaagtgtt tgtgaccctc acctgcgcct tccgctatgg ccgtgaagac      660

ctggatgtgc tgggcttgtc cttccgcaaa gacctgttca tcgccaccta ccaggccttc      720

cccccggtgc ccaacccacc ccggcccccc acccgcctgc aggaccggct gctgaggaag      780

ctgggccagc atgcccaccc cttcttcttc accatacccc agaatcttcc atgctccgtc      840

acactgcagc caggcccaga ggatacagga aaggcctgcg gcgtagactt tgagattcga      900

gccttctgtg ctaaatcact agaagagaaa agccacaaaa ggaactctgt gcggctggtg      960

atccgaaagg tgcagttcgc cccggagaaa cccggccccc agccttcagc cgaaaccaca     1020

cgccacttcc tcatgtctga ccggtccctg cacctcgagg cttccctgga caaggagctg     1080

tactaccatg gggagcccct caatgtaaat gtccacgtca ccaacaactc caccaagacc     1140

gtcaagaaga tcaaagtctc tgtgagacag tacgccgaca tctgcctctt cagcaccgcc     1200

cagtacaagt gtcctgtggc tcaactcgaa caagatgacc aggtatctcc cagctccaca     1260

ttctgtaagg tgtacaccat aaccccactg ctcagcgaca accgggagaa gcggggtctc     1320

gccctggatg ggaaactcaa gcacgaggac accaacctgg cttccagcac catcgtgaag     1380

gagggtgcca acaaggaggt gctgggaatc ctggtgtcct acagggtcaa ggtgaagctg     1440

gtggtgtctc gaggcgggga tgtctctgtg gagctgcctt ttgttcttat gcaccccaag     1500

ccccacgacc acatccccct ccccagaccc cagtcagccg ctccggagac agatgtccct     1560

gtggacacca acctcattga atttgatacc aactatgcca cagatgatga cattgtgttt     1620

gaggactttg cccggcttcg gctgaagggg atgaaggatg acgactatga tgatcaactc     1680

tgcaatgttt cgaagaagac ttaccccaac gacaaaacca tcatcagtac ctttaagtgg     1740

agttacacca ctggaaatgg caagcgctac cggagcactg cgcggaccac ctacaccttt     1800

gccaagccaa tggcggctaa ctatctgaag aaccagccga tgtacgtgtt ccgtaagacg     1860

gagctcaagc actccaagac cgagctcaac ttcaaggagt ggcaaaaggc ctttaccgat     1920

gtgatgggca tggacgagct gtacaagtag                                      1950


<210>  53
<211>  649
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr L1-3B8 - coding sequence


<220>
<221>  SITE
<222>  (1)..(150)
<223>  mNeonGreen Part 1

<220>
<221>  SITE
<222>  (151)..(154)
<223>  N-terminal linker

<220>
<221>  SITE
<222>  (154)..(561)
<223>  B-Arrestin

<220>
<221>  SITE
<222>  (562)..(563)
<223>  C-terminal linker

<220>
<221>  SITE
<222>  (564)..(649)
<223>  mNeonGreen Part 2

<400>  53

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr 
1               5                   10                  15      


His Glu Leu His Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met 
            20                  25                  30          


Val Gly Gln Gly Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn 
        35                  40                  45              


Leu Lys Ser Thr Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val 
    50                  55                  60                  


Pro His Ile Gly Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly 
65                  70                  75                  80  


Met Ser Pro Phe Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val 
                85                  90                  95      


His Arg Thr Met Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr 
            100                 105                 110         


Arg Tyr Thr Tyr Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys 
        115                 120                 125             


Gly Thr Gly Phe Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr 
    130                 135                 140                 


Ala Ala Asp Trp Cys Arg Ile Leu Trp Gly Glu Lys Pro Gly Thr Arg 
145                 150                 155                 160 


Val Phe Lys Lys Ser Ser Pro Asn Cys Lys Leu Thr Val Tyr Leu Gly 
                165                 170                 175     


Lys Arg Asp Phe Val Asp His Leu Asp Lys Val Asp Pro Val Asp Gly 
            180                 185                 190         


Val Val Leu Val Asp Pro Asp Tyr Leu Lys Asp Arg Lys Val Phe Val 
        195                 200                 205             


Thr Leu Thr Cys Ala Phe Arg Tyr Gly Arg Glu Asp Leu Asp Val Leu 
    210                 215                 220                 


Gly Leu Ser Phe Arg Lys Asp Leu Phe Ile Ala Thr Tyr Gln Ala Phe 
225                 230                 235                 240 


Pro Pro Val Pro Asn Pro Pro Arg Pro Pro Thr Arg Leu Gln Asp Arg 
                245                 250                 255     


Leu Leu Arg Lys Leu Gly Gln His Ala His Pro Phe Phe Phe Thr Ile 
            260                 265                 270         


Pro Gln Asn Leu Pro Cys Ser Val Thr Leu Gln Pro Gly Pro Glu Asp 
        275                 280                 285             


Thr Gly Lys Ala Cys Gly Val Asp Phe Glu Ile Arg Ala Phe Cys Ala 
    290                 295                 300                 


Lys Ser Leu Glu Glu Lys Ser His Lys Arg Asn Ser Val Arg Leu Val 
305                 310                 315                 320 


Ile Arg Lys Val Gln Phe Ala Pro Glu Lys Pro Gly Pro Gln Pro Ser 
                325                 330                 335     


Ala Glu Thr Thr Arg His Phe Leu Met Ser Asp Arg Ser Leu His Leu 
            340                 345                 350         


Glu Ala Ser Leu Asp Lys Glu Leu Tyr Tyr His Gly Glu Pro Leu Asn 
        355                 360                 365             


Val Asn Val His Val Thr Asn Asn Ser Thr Lys Thr Val Lys Lys Ile 
    370                 375                 380                 


Lys Val Ser Val Arg Gln Tyr Ala Asp Ile Cys Leu Phe Ser Thr Ala 
385                 390                 395                 400 


Gln Tyr Lys Cys Pro Val Ala Gln Leu Glu Gln Asp Asp Gln Val Ser 
                405                 410                 415     


Pro Ser Ser Thr Phe Cys Lys Val Tyr Thr Ile Thr Pro Leu Leu Ser 
            420                 425                 430         


Asp Asn Arg Glu Lys Arg Gly Leu Ala Leu Asp Gly Lys Leu Lys His 
        435                 440                 445             


Glu Asp Thr Asn Leu Ala Ser Ser Thr Ile Val Lys Glu Gly Ala Asn 
    450                 455                 460                 


Lys Glu Val Leu Gly Ile Leu Val Ser Tyr Arg Val Lys Val Lys Leu 
465                 470                 475                 480 


Val Val Ser Arg Gly Gly Asp Val Ser Val Glu Leu Pro Phe Val Leu 
                485                 490                 495     


Met His Pro Lys Pro His Asp His Ile Pro Leu Pro Arg Pro Gln Ser 
            500                 505                 510         


Ala Ala Pro Glu Thr Asp Val Pro Val Asp Thr Asn Leu Ile Glu Phe 
        515                 520                 525             


Asp Thr Asn Tyr Ala Thr Asp Asp Asp Ile Val Phe Glu Asp Phe Ala 
    530                 535                 540                 


Arg Leu Arg Leu Lys Gly Met Lys Asp Asp Asp Tyr Asp Asp Gln Leu 
545                 550                 555                 560 


Cys Asn Val Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser 
                565                 570                 575     


Thr Phe Lys Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser 
            580                 585                 590         


Thr Ala Arg Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr 
        595                 600                 605             


Leu Lys Asn Gln Pro Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His 
    610                 615                 620                 


Ser Lys Thr Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp 
625                 630                 635                 640 


Val Met Gly Met Asp Glu Leu Tyr Lys 
                645                 


<210>  54
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr L1-3B8 - mNeonGreen Part 1


<220>
<221>  misc_feature
<222>  (1)..(450)
<223>  mNeonGreen Part 1

<400>  54
atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca tgagttacac       60

atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac cggcaatcca      120

aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc      180

tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta ccctgacggg      240

atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca tcgcacaatg      300

cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga gggaagccac      360

atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc      420

aactcgctga ccgctgcgga ctggtgcagg                                       450


<210>  55
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr L1-3B8 - mNeonGreen Part 1


<220>
<221>  SITE
<222>  (1)..(150)
<223>  mNeonGreen Part 1

<400>  55

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ser Leu Pro Ala Thr 
1               5                   10                  15      


His Glu Leu His Ile Phe Gly Ser Ile Asn Gly Val Asp Phe Asp Met 
            20                  25                  30          


Val Gly Gln Gly Thr Gly Asn Pro Asn Asp Gly Tyr Glu Glu Leu Asn 
        35                  40                  45              


Leu Lys Ser Thr Lys Gly Asp Leu Gln Phe Ser Pro Trp Ile Leu Val 
    50                  55                  60                  


Pro His Ile Gly Tyr Gly Phe His Gln Tyr Leu Pro Tyr Pro Asp Gly 
65                  70                  75                  80  


Met Ser Pro Phe Gln Ala Ala Met Val Asp Gly Ser Gly Tyr Gln Val 
                85                  90                  95      


His Arg Thr Met Gln Phe Glu Asp Gly Ala Ser Leu Thr Val Asn Tyr 
            100                 105                 110         


Arg Tyr Thr Tyr Glu Gly Ser His Ile Lys Gly Glu Ala Gln Val Lys 
        115                 120                 125             


Gly Thr Gly Phe Pro Ala Asp Gly Pro Val Met Thr Asn Ser Leu Thr 
    130                 135                 140                 


Ala Ala Asp Trp Cys Arg 
145                 150 


<210>  56
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr L1-3B8 - 1st linker sequence


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  N-terminal linker

<400>  56
atcctttggg gg                                                           12


<210>  57
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr L1-3B8 - 1st Linker Sequence

<400>  57

Ile Leu Trp Gly 
1               


<210>  58

<400>  58
000

<210>  59
<211>  408
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr L1-3B8 - Beta-arrestin-2


<220>
<221>  SITE
<222>  (1)..(408)
<223>  B-Arrestin

<400>  59

Gly Glu Lys Pro Gly Thr Arg Val Phe Lys Lys Ser Ser Pro Asn Cys 
1               5                   10                  15      


Lys Leu Thr Val Tyr Leu Gly Lys Arg Asp Phe Val Asp His Leu Asp 
            20                  25                  30          


Lys Val Asp Pro Val Asp Gly Val Val Leu Val Asp Pro Asp Tyr Leu 
        35                  40                  45              


Lys Asp Arg Lys Val Phe Val Thr Leu Thr Cys Ala Phe Arg Tyr Gly 
    50                  55                  60                  


Arg Glu Asp Leu Asp Val Leu Gly Leu Ser Phe Arg Lys Asp Leu Phe 
65                  70                  75                  80  


Ile Ala Thr Tyr Gln Ala Phe Pro Pro Val Pro Asn Pro Pro Arg Pro 
                85                  90                  95      


Pro Thr Arg Leu Gln Asp Arg Leu Leu Arg Lys Leu Gly Gln His Ala 
            100                 105                 110         


His Pro Phe Phe Phe Thr Ile Pro Gln Asn Leu Pro Cys Ser Val Thr 
        115                 120                 125             


Leu Gln Pro Gly Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp Phe 
    130                 135                 140                 


Glu Ile Arg Ala Phe Cys Ala Lys Ser Leu Glu Glu Lys Ser His Lys 
145                 150                 155                 160 


Arg Asn Ser Val Arg Leu Val Ile Arg Lys Val Gln Phe Ala Pro Glu 
                165                 170                 175     


Lys Pro Gly Pro Gln Pro Ser Ala Glu Thr Thr Arg His Phe Leu Met 
            180                 185                 190         


Ser Asp Arg Ser Leu His Leu Glu Ala Ser Leu Asp Lys Glu Leu Tyr 
        195                 200                 205             


Tyr His Gly Glu Pro Leu Asn Val Asn Val His Val Thr Asn Asn Ser 
    210                 215                 220                 


Thr Lys Thr Val Lys Lys Ile Lys Val Ser Val Arg Gln Tyr Ala Asp 
225                 230                 235                 240 


Ile Cys Leu Phe Ser Thr Ala Gln Tyr Lys Cys Pro Val Ala Gln Leu 
                245                 250                 255     


Glu Gln Asp Asp Gln Val Ser Pro Ser Ser Thr Phe Cys Lys Val Tyr 
            260                 265                 270         


Thr Ile Thr Pro Leu Leu Ser Asp Asn Arg Glu Lys Arg Gly Leu Ala 
        275                 280                 285             


Leu Asp Gly Lys Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser Thr 
    290                 295                 300                 


Ile Val Lys Glu Gly Ala Asn Lys Glu Val Leu Gly Ile Leu Val Ser 
305                 310                 315                 320 


Tyr Arg Val Lys Val Lys Leu Val Val Ser Arg Gly Gly Asp Val Ser 
                325                 330                 335     


Val Glu Leu Pro Phe Val Leu Met His Pro Lys Pro His Asp His Ile 
            340                 345                 350         


Pro Leu Pro Arg Pro Gln Ser Ala Ala Pro Glu Thr Asp Val Pro Val 
        355                 360                 365             


Asp Thr Asn Leu Ile Glu Phe Asp Thr Asn Tyr Ala Thr Asp Asp Asp 
    370                 375                 380                 


Ile Val Phe Glu Asp Phe Ala Arg Leu Arg Leu Lys Gly Met Lys Asp 
385                 390                 395                 400 


Asp Asp Tyr Asp Asp Gln Leu Cys 
                405             


<210>  60
<211>  261
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BArr L1-3B8 - mNeonGreen Part 2


<220>
<221>  misc_feature
<222>  (1)..(261)
<223>  mNeonGreen Part 2

<400>  60
tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc       60

actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca      120

atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag      180

cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc      240

atggacgagc tgtacaagta g                                                261


<210>  61
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BArr L1-3B8 - mNeonGreen Part 2


<220>
<221>  SITE
<222>  (1)..(86)
<223>  mNeonGreen Part 2

<400>  61

Ser Lys Lys Thr Tyr Pro Asn Asp Lys Thr Ile Ile Ser Thr Phe Lys 
1               5                   10                  15      


Trp Ser Tyr Thr Thr Gly Asn Gly Lys Arg Tyr Arg Ser Thr Ala Arg 
            20                  25                  30          


Thr Thr Tyr Thr Phe Ala Lys Pro Met Ala Ala Asn Tyr Leu Lys Asn 
        35                  40                  45              


Gln Pro Met Tyr Val Phe Arg Lys Thr Glu Leu Lys His Ser Lys Thr 
    50                  55                  60                  


Glu Leu Asn Phe Lys Glu Trp Gln Lys Ala Phe Thr Asp Val Met Gly 
65                  70                  75                  80  


Met Asp Glu Leu Tyr Lys 
                85      



