                         SEQUENCE LISTING

<110>  GSK Biologicals SA
 
<120>  Antigen Purification Method

<130>  VU66634FF

<150>  62712280
<151>  2018-07-31

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  544
<212>  PRT
<213>  RSV

<400>  1

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr 
1               5                   10                  15      


Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro 
            100                 105                 110         


Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr 
        115                 120                 125             


Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val 
    130                 135                 140                 


Gly Ser Ala Ile Ala Ser Gly Val Ala Val Cys Lys Val Leu His Leu 
145                 150                 155                 160 


Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 
                165                 170                 175     


Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Phe Lys Val 
            180                 185                 190         


Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Leu Asn 
        195                 200                 205             


Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln 
    210                 215                 220                 


Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn 
225                 230                 235                 240 


Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 
                245                 250                 255     


Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys 
            260                 265                 270         


Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile 
        275                 280                 285             


Met Cys Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro 
    290                 295                 300                 


Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro 
305                 310                 315                 320 


Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg 
                325                 330                 335     


Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe 
            340                 345                 350         


Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp 
        355                 360                 365             


Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val 
    370                 375                 380                 


Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr 
385                 390                 395                 400 


Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys 
                405                 410                 415     


Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile 
            420                 425                 430         


Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp 
        435                 440                 445             


Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly 
    450                 455                 460                 


Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro 
465                 470                 475                 480 


Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn 
                485                 490                 495     


Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu 
            500                 505                 510         


Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln 
        515                 520                 525             


Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
    530                 535                 540                 


<210>  2
<211>  408
<212>  PRT
<213>  RSV

<400>  2

Phe Leu Gly Phe Leu Leu Gly Val Gly Ser Ala Ile Ala Ser Gly Val 
1               5                   10                  15      


Ala Val Cys Lys Val Leu His Leu Glu Gly Glu Val Asn Lys Ile Lys 
            20                  25                  30          


Ser Ala Leu Leu Ser Thr Asn Lys Ala Val Val Ser Leu Ser Asn Gly 
        35                  40                  45              


Val Ser Val Leu Thr Phe Lys Val Leu Asp Leu Lys Asn Tyr Ile Asp 
    50                  55                  60                  


Lys Gln Leu Leu Pro Ile Leu Asn Lys Gln Ser Cys Ser Ile Ser Asn 
65                  70                  75                  80  


Ile Glu Thr Val Ile Glu Phe Gln Gln Lys Asn Asn Arg Leu Leu Glu 
                85                  90                  95      


Ile Thr Arg Glu Phe Ser Val Asn Ala Gly Val Thr Thr Pro Val Ser 
            100                 105                 110         


Thr Tyr Met Leu Thr Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp Met 
        115                 120                 125             


Pro Ile Thr Asn Asp Gln Lys Lys Leu Met Ser Asn Asn Val Gln Ile 
    130                 135                 140                 


Val Arg Gln Gln Ser Tyr Ser Ile Met Cys Ile Ile Lys Glu Glu Val 
145                 150                 155                 160 


Leu Ala Tyr Val Val Gln Leu Pro Leu Tyr Gly Val Ile Asp Thr Pro 
                165                 170                 175     


Cys Trp Lys Leu His Thr Ser Pro Leu Cys Thr Thr Asn Thr Lys Glu 
            180                 185                 190         


Gly Ser Asn Ile Cys Leu Thr Arg Thr Asp Arg Gly Trp Tyr Cys Asp 
        195                 200                 205             


Asn Ala Gly Ser Val Ser Phe Phe Pro Gln Ala Glu Thr Cys Lys Val 
    210                 215                 220                 


Gln Ser Asn Arg Val Phe Cys Asp Thr Met Asn Ser Leu Thr Leu Pro 
225                 230                 235                 240 


Ser Glu Val Asn Leu Cys Asn Val Asp Ile Phe Asn Pro Lys Tyr Asp 
                245                 250                 255     


Cys Lys Ile Met Thr Ser Lys Thr Asp Val Ser Ser Ser Val Ile Thr 
            260                 265                 270         


Ser Leu Gly Ala Ile Val Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala 
        275                 280                 285             


Ser Asn Lys Asn Arg Gly Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp 
    290                 295                 300                 


Tyr Val Ser Asn Lys Gly Val Asp Thr Val Ser Val Gly Asn Thr Leu 
305                 310                 315                 320 


Tyr Tyr Val Asn Lys Gln Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu 
                325                 330                 335     


Pro Ile Ile Asn Phe Tyr Asp Pro Leu Val Phe Pro Ser Asp Glu Phe 
            340                 345                 350         


Asp Ala Ser Ile Ser Gln Val Asn Glu Lys Ile Asn Gln Ser Leu Ala 
        355                 360                 365             


Phe Ile Arg Lys Ser Asp Glu Leu Leu Ser Ala Ile Gly Gly Tyr Ile 
    370                 375                 380                 


Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu 
385                 390                 395                 400 


Trp Val Leu Leu Ser Thr Phe Leu 
                405             


<210>  3
<211>  84
<212>  PRT
<213>  RSV

<400>  3

Gln Asn Ile Thr Glu Glu Phe Tyr Gln Ser Thr Cys Ser Ala Val Ser 
1               5                   10                  15      


Lys Gly Tyr Leu Ser Ala Leu Arg Thr Gly Trp Tyr Thr Ser Val Ile 
            20                  25                  30          


Thr Ile Glu Leu Ser Asn Ile Lys Glu Asn Lys Cys Asn Gly Thr Asp 
        35                  40                  45              


Ala Lys Val Lys Leu Ile Lys Gln Glu Leu Asp Lys Tyr Lys Asn Ala 
    50                  55                  60                  


Val Thr Glu Leu Gln Leu Leu Met Gln Ser Thr Pro Ala Thr Asn Asn 
65                  70                  75                  80  


Arg Ala Arg Arg 
                


<210>  4
<211>  11
<212>  PRT
<213>  RSV

<400>  4

Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys 
1               5                   10      


<210>  5
<211>  11
<212>  PRT
<213>  RSV

<400>  5

Lys Ile Asp Gln Ser Leu Ala Phe Ile Arg Lys 
1               5                   10      


<210>  6
<211>  10
<212>  PRT
<213>  RSV

<400>  6

Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys 
1               5                   10  


<210>  7
<211>  27
<212>  PRT
<213>  RSV

<400>  7

Glu Leu Pro Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr 
1               5                   10                  15      


Asn Val Thr Leu Ser Lys Lys Arg Lys Arg Arg 
            20                  25          


<210>  8
<211>  435
<212>  PRT
<213>  RSV

<400>  8

Glu Leu Pro Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr 
1               5                   10                  15      


Asn Val Thr Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu 
            20                  25                  30          


Leu Gly Val Gly Ser Ala Ile Ala Ser Gly Val Ala Val Cys Lys Val 
        35                  40                  45              


Leu His Leu Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser 
    50                  55                  60                  


Thr Asn Lys Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr 
65                  70                  75                  80  


Phe Lys Val Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro 
                85                  90                  95      


Ile Leu Asn Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile 
            100                 105                 110         


Glu Phe Gln Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe 
        115                 120                 125             


Ser Val Asn Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr 
    130                 135                 140                 


Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp 
145                 150                 155                 160 


Gln Lys Lys Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser 
                165                 170                 175     


Tyr Ser Ile Met Cys Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val 
            180                 185                 190         


Gln Leu Pro Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His 
        195                 200                 205             


Thr Ser Pro Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys 
    210                 215                 220                 


Leu Thr Arg Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val 
225                 230                 235                 240 


Ser Phe Phe Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val 
                245                 250                 255     


Phe Cys Asp Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu 
            260                 265                 270         


Cys Asn Val Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr 
        275                 280                 285             


Ser Lys Thr Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile 
    290                 295                 300                 


Val Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg 
305                 310                 315                 320 


Gly Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys 
                325                 330                 335     


Gly Val Asp Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys 
            340                 345                 350         


Gln Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe 
        355                 360                 365             


Tyr Asp Pro Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser 
    370                 375                 380                 


Gln Val Asn Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser 
385                 390                 395                 400 


Asp Glu Leu Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg 
                405                 410                 415     


Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser 
            420                 425                 430         


Thr Phe Leu 
        435 


<210>  9
<211>  5
<212>  PRT
<213>  RSV


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  9

Arg Xaa Arg Arg Xaa 
1               5   


