                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  Nucleic acid construct

<130>  P115569PCT

<140>  PCT/GB2019/052150
<141>  2019-07-31

<150>  GB 1812474.3
<151>  2018-07-31

<160>  59    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  frame-slip motif

<400>  1
uuuuuuu                                                                  7


<210>  2
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  frame-slip motif

<400>  2
uuuuuuuga                                                                9


<210>  3
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  frame-slip motif

<400>  3
uuuuuuuag                                                                9


<210>  4
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  frame-slip motif

<400>  4
uuuuuuuaa                                                                9


<210>  5
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  translational readthrough motif

<400>  5
ugacuag                                                                  7


<210>  6
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  translational readthrough motif

<400>  6
uagcuag                                                                  7


<210>  7
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  translational readthrough motif

<400>  7
uaacuag                                                                  7


<210>  8
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  translational readthrough motif

<400>  8
ugacaauua                                                                9


<210>  9
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  translational readthrough motif

<400>  9
uagcaauua                                                                9


<210>  10
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  translational readthrough motif

<400>  10
uaacaauua                                                                9


<210>  11
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  multiple stop translational readthrough motif

<400>  11
ugaugacuag                                                              10


<210>  12
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  12

Asp Met Glu Ser Asn Pro Gly Pro 
1               5               


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  13

Asp Val Glu Leu Asn Pro Gly Pro 
1               5               


<210>  14
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  14

Asp Val Glu Glu Asn Pro Gly Pro 
1               5               


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  15

Asp Ile Glu Leu Asn Pro Gly Pro 
1               5               


<210>  16
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  16

Asp Ile Glu Gln Asn Pro Gly Pro 
1               5               


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  17

Asp Ser Glu Phe Asn Pro Gly Pro 
1               5               


<210>  18
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  18

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  19
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  19

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Gln Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  20
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  20

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ile Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  21
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  21

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Phe Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  22
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  22

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser His 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  23
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  23

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Glu 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  24
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  24

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Gln 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  25
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  25

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Gly 
            


<210>  26
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  26

Tyr His Ala Asp Tyr Tyr Lys Gln Arg Leu Ile His Asp Val Glu Met 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  27
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  27

His Tyr Ala Gly Tyr Phe Ala Asp Leu Leu Ile His Asp Ile Glu Thr 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  28
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  28

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  29
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  29

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  30
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  30

Ala Ala Arg Gln Met Leu Leu Leu Leu Ser Gly Asp Val Glu Thr Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  31
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  31

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  32
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  32

Thr Arg Ala Glu Ile Glu Asp Glu Leu Ile Arg Ala Gly Ile Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  33
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  33

Thr Arg Ala Glu Ile Glu Asp Glu Leu Ile Arg Ala Asp Ile Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  34
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  34

Ala Lys Phe Gln Ile Asp Lys Ile Leu Ile Ser Gly Asp Val Glu Leu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  35
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  35

Ser Ser Ile Ile Arg Thr Lys Met Leu Val Ser Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  36
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  36

Cys Asp Ala Gln Arg Gln Lys Leu Leu Leu Ser Gly Asp Ile Glu Gln 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  37
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  37

Tyr Pro Ile Asp Phe Gly Gly Phe Leu Val Lys Ala Asp Ser Glu Phe 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  38
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  38

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile 
            20                  25                  30          


Val Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
        35                  40                  45              


Gly Asp Val Glu Ser Asn Pro Gly Pro 
    50                  55          


<210>  39
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  39

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  40
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  40

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  41
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  41

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  42
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  murine Ig kappa chain V-III region signal peptide (wild-type)

<400>  42

Met Glu Thr Asp Thr Leu Ile Leu Trp Val Leu Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  43
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  murine Ig kappa chain V-III region signal peptide (K9 mutant)

<400>  43

Met Glu Thr Asp Thr Leu Ile Leu Lys Val Leu Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  44
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  murine Ig kappa chain V-III region signal peptide (K10 mutant)

<400>  44

Met Glu Thr Asp Thr Leu Ile Leu Trp Lys Leu Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  45
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  murine Ig kappa chain V-III region signal peptide (K11 mutant)

<400>  45

Met Glu Thr Asp Thr Leu Ile Leu Trp Val Lys Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  46
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  46

Asp Ile Glu Thr Asn Pro Gly Pro 
1               5               


<210>  47
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  47

Asp Val Glu Thr Asn Pro Gly Pro 
1               5               


<210>  48
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  48

Asp Val Glu Met Asn Pro Gly Pro 
1               5               


<210>  49
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site motif

<400>  49

Asp Val Glu Ser Asn Pro Gly Pro 
1               5               


<210>  50
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  basic amino acid furin target sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa may be Arg or Lys

<400>  50

Arg Xaa Xaa Arg 
1               


<210>  51
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus Tobacco Etch Virus (TEV) cleavage site

<400>  51

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  52
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa may be any amino acid or may be selected from the group: I, 
       V, M and S

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa may be any amino acid or may be selected from the group: T, 
       M, S, L, E, Q and F

<400>  52

Asp Xaa Glu Xaa Asn Pro Gly Pro 
1               5               


<210>  53
<211>  197
<212>  PRT
<213>  Homo sapiens

<400>  53

Arg Asn Leu Pro Val Ala Thr Pro Asp Pro Gly Met Phe Pro Cys Leu 
1               5                   10                  15      


His His Ser Gln Asn Leu Leu Arg Ala Val Ser Asn Met Leu Gln Lys 
            20                  25                  30          


Ala Arg Gln Thr Leu Glu Phe Tyr Pro Cys Thr Ser Glu Glu Ile Asp 
        35                  40                  45              


His Glu Asp Ile Thr Lys Asp Lys Thr Ser Thr Val Glu Ala Cys Leu 
    50                  55                  60                  


Pro Leu Glu Leu Thr Lys Asn Glu Ser Cys Leu Asn Ser Arg Glu Thr 
65                  70                  75                  80  


Ser Phe Ile Thr Asn Gly Ser Cys Leu Ala Ser Arg Lys Thr Ser Phe 
                85                  90                  95      


Met Met Ala Leu Cys Leu Ser Ser Ile Tyr Glu Asp Leu Lys Met Tyr 
            100                 105                 110         


Gln Val Glu Phe Lys Thr Met Asn Ala Lys Leu Leu Met Asp Pro Lys 
        115                 120                 125             


Arg Gln Ile Phe Leu Asp Gln Asn Met Leu Ala Val Ile Asp Glu Leu 
    130                 135                 140                 


Met Gln Ala Leu Asn Phe Asn Ser Glu Thr Val Pro Gln Lys Ser Ser 
145                 150                 155                 160 


Leu Glu Glu Pro Asp Phe Tyr Lys Thr Lys Ile Lys Leu Cys Ile Leu 
                165                 170                 175     


Leu His Ala Phe Arg Ile Arg Ala Val Thr Ile Asp Arg Val Met Ser 
            180                 185                 190         


Tyr Leu Asn Ala Ser 
        195         


<210>  54
<211>  304
<212>  PRT
<213>  Homo sapiens

<400>  54

Trp Glu Leu Lys Lys Asp Val Tyr Val Val Glu Leu Asp Trp Tyr Pro 
1               5                   10                  15      


Asp Ala Pro Gly Glu Met Val Val Leu Thr Cys Asp Thr Pro Glu Glu 
            20                  25                  30          


Asp Gly Ile Thr Trp Thr Leu Asp Gln Ser Ser Glu Val Leu Gly Ser 
        35                  40                  45              


Gly Lys Thr Leu Thr Ile Gln Val Lys Glu Phe Gly Asp Ala Gly Gln 
    50                  55                  60                  


Tyr Thr Cys His Lys Gly Gly Glu Val Leu Ser His Ser Leu Leu Leu 
65                  70                  75                  80  


Leu His Lys Lys Glu Asp Gly Ile Trp Ser Thr Asp Ile Leu Lys Asp 
                85                  90                  95      


Gln Lys Glu Pro Lys Asn Lys Thr Phe Leu Arg Cys Glu Ala Lys Asn 
            100                 105                 110         


Tyr Ser Gly Arg Phe Thr Cys Trp Trp Leu Thr Thr Ile Ser Thr Asp 
        115                 120                 125             


Leu Thr Phe Ser Val Lys Ser Ser Arg Gly Ser Ser Asp Pro Gln Gly 
    130                 135                 140                 


Val Thr Cys Gly Ala Ala Thr Leu Ser Ala Glu Arg Val Arg Gly Asp 
145                 150                 155                 160 


Asn Lys Glu Tyr Glu Tyr Ser Val Glu Cys Gln Glu Asp Ser Ala Cys 
                165                 170                 175     


Pro Ala Ala Glu Glu Ser Leu Pro Ile Glu Val Met Val Asp Ala Val 
            180                 185                 190         


His Lys Leu Lys Tyr Glu Asn Tyr Thr Ser Ser Phe Phe Ile Arg Asp 
        195                 200                 205             


Ile Ile Lys Pro Asp Pro Pro Lys Asn Leu Gln Leu Lys Pro Leu Lys 
    210                 215                 220                 


Asn Ser Arg Gln Val Glu Val Ser Trp Glu Tyr Pro Asp Thr Trp Ser 
225                 230                 235                 240 


Thr Pro His Ser Tyr Phe Ser Leu Thr Phe Cys Val Gln Val Gln Gly 
                245                 250                 255     


Lys Ser Lys Arg Glu Lys Lys Asp Arg Val Phe Thr Asp Lys Thr Ser 
            260                 265                 270         


Ala Thr Val Ile Cys Arg Lys Asn Ala Ser Ile Ser Val Arg Ala Gln 
        275                 280                 285             


Asp Arg Tyr Tyr Ser Ser Ser Trp Ser Glu Trp Ala Ser Val Pro Cys 
    290                 295                 300                 


<210>  55
<211>  540
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a flexi-IL-12 sequence

<400>  55

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Met Trp Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          


Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              


Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  


Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  


Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      


Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         


Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             


Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 


Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     


Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         


Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             


Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 


Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 


Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     


Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         


Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             


Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 


Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 


Glu Trp Ala Ser Val Pro Cys Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                325                 330                 335     


Gly Ser Gly Gly Gly Gly Ser Arg Asn Leu Pro Leu Ala Thr Pro Asp 
            340                 345                 350         


Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala 
        355                 360                 365             


Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro 
    370                 375                 380                 


Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr 
385                 390                 395                 400 


Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser 
                405                 410                 415     


Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu 
            420                 425                 430         


Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile 
        435                 440                 445             


Tyr Glu Asp Ser Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala 
    450                 455                 460                 


Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met 
465                 470                 475                 480 


Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu 
                485                 490                 495     


Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr 
            500                 505                 510         


Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val 
        515                 520                 525             


Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser 
    530                 535                 540 


<210>  56
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from human common gamma chain

<400>  56

Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys Asn Leu Glu Asp Leu 
1               5                   10                  15      


Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp Ser Gly Val Ser Lys 
            20                  25                  30          


Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser Glu Arg Leu Cys Leu 
        35                  40                  45              


Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly 
    50                  55                  60                  


Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp Ala Pro Pro Cys Tyr 
65                  70                  75                  80  


Thr Leu Lys Pro Glu Thr 
                85      


<210>  57
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from human IL-2Rbeta

<400>  57

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
                165                 170                 175     


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro 
            180                 185                 190         


Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly 
        195                 200                 205             


Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro 
    210                 215                 220                 


Glu Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro 
225                 230                 235                 240 


Arg Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu 
                245                 250                 255     


Phe Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu 
            260                 265                 270         


Ser Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
        275                 280                 285     


<210>  58
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  kappa chain constant domain

<400>  58

Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
1               5                   10                  15      


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
            20                  25                  30          


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
        35                  40                  45              


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
    50                  55                  60                  


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
65                  70                  75                  80  


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
                85                  90                  95      


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105     


<210>  59
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain constant domain (CH) sequence from a gamma 
       immunoglobulin heavy chain

<400>  59

Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
1               5                   10                  15      


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
            20                  25                  30          


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
        35                  40                  45              


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
    50                  55                  60                  


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
65                  70                  75                  80  


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
                85                  90                  95      


Val 
    


