                         SEQUENCE LISTING

<110>  Oxford University Innovation Limited
 
<120>  Assemblies

<130>  N414111WO

<160>  118   

<170>  PatentIn version 3.5

<210>  1
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CWza (T376R)

<400>  1

Cys Gly Gly Ala Pro Leu Val Arg Trp Asn Arg Val Ile Ser Gln Leu 
1               5                   10                  15      


Val Pro Thr Ile Ser Gly Val His Asp Met Thr Glu Thr Val Arg Tyr 
            20                  25                  30          


Ile Lys Arg Trp Pro Asn 
        35              


<210>  2
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conjugation CWza (T376R) from paper

<400>  2

Cys Gly Gly Ala Pro Leu Val Arg Trp Asn Arg Val Ile Ser Gln Leu 
1               5                   10                  15      


Val Pro Thr Ile Thr Gly Val His Asp Leu Thr Glu Thr Val Arg Tyr 
            20                  25                  30          


Ile Lys Arg Trp Pro Asn 
        35              


<210>  3
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WzaC (T376R)

<400>  3

Ala Pro Leu Val Arg Trp Asn Arg Val Ile Ser Gln Leu Val Pro Thr 
1               5                   10                  15      


Ile Ser Gly Val His Asp Met Thr Glu Thr Val Arg Tyr Ile Lys Arg 
            20                  25                  30          


Trp Pro Asn Gly Gly Cys 
        35              


<210>  4
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Conjugation WzaC (T376R) from paper

<400>  4

Ala Pro Leu Val Arg Trp Asn Arg Val Ile Ser Gln Leu Val Pro Thr 
1               5                   10                  15      


Ile Thr Gly Val His Asp Leu Thr Glu Thr Val Arg Tyr Ile Lys Arg 
            20                  25                  30          


Trp Pro Asn Gly Gly Cys 
        35              


<210>  5
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wza E.coli (R376T)

<400>  5

Ala Pro Leu Val Arg Trp Asn Arg Val Ile Ser Gln Leu Val Pro Thr 
1               5                   10                  15      


Ile Ser Gly Val His Asp Met Thr Glu Thr Val Arg Tyr Ile Lys Thr 
            20                  25                  30          


Trp Pro Asn 
        35  


<210>  6
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KRM consensus Wza (R376T)

<400>  6

Ala Pro Leu Val Arg Trp Asn Arg Val Ile Ser Gln Leu Val Pro Thr 
1               5                   10                  15      


Ile Thr Gly Val His Asp Leu Thr Glu Thr Val Arg Tyr Ile Lys Thr 
            20                  25                  30          


Trp Pro Asn 
        35  


<210>  7
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KRM consensus Wza K375C (R376T)

<400>  7

Ala Pro Leu Val Arg Trp Asn Arg Val Ile Ser Gln Leu Val Pro Thr 
1               5                   10                  15      


Ile Thr Gly Val His Asp Leu Thr Glu Thr Val Arg Tyr Ile Cys Thr 
            20                  25                  30          


Trp Pro Asn 
        35  


<210>  8
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KRM consensus Wza Y373C (R376T)

<400>  8

Ala Pro Leu Val Arg Trp Asn Arg Val Ile Ser Gln Leu Val Pro Thr 
1               5                   10                  15      


Ile Thr Gly Val His Asp Leu Thr Glu Thr Val Arg Cys Ile Lys Thr 
            20                  25                  30          


Trp Pro Asn 
        35  


<210>  9
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  alpha HL-UP (111-147, T129C)

<400>  9

Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly 
1               5                   10                  15      


Asp Asp Cys Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile 
            20                  25                  30          


Gly His Thr Leu Lys 
        35          


<210>  10
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  alpha HL-DOWN

<400>  10

Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu 
1               5                   10                  15      


Lys Gly Cys Asp Asp Gly Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn 
            20                  25                  30          


Gly Asn Val Thr Asp 
        35          


<210>  11
<211>  319
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  alpha hemolysin protein sequence (inc leader sequence)

<400>  11

Met Lys Thr Arg Ile Val Ser Ser Val Thr Thr Thr Leu Leu Leu Gly 
1               5                   10                  15      


Ser Ile Leu Met Asn Pro Val Ala Gly Ala Ala Asp Ser Asp Ile Asn 
            20                  25                  30          


Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser Asn Thr Thr Val Lys Thr 
        35                  40                  45              


Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn Gly Met His Lys Lys Val 
    50                  55                  60                  


Phe Tyr Ser Phe Ile Asp Asp Lys Asn His Asn Lys Lys Leu Leu Val 
65                  70                  75                  80  


Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu 
                85                  90                  95      


Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp Pro Ser Ala Phe Lys Val 
            100                 105                 110         


Gln Leu Gln Leu Pro Asp Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr 
        115                 120                 125             


Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr 
    130                 135                 140                 


Gly Phe Asn Gly Asn Val Thr Gly Asp Asp Thr Gly Lys Ile Gly Gly 
145                 150                 155                 160 


Leu Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln 
                165                 170                 175     


Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly 
            180                 185                 190         


Trp Lys Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr 
        195                 200                 205             


Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys 
    210                 215                 220                 


Thr Arg Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn 
225                 230                 235                 240 


Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr 
                245                 250                 255     


Val Ile Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp 
            260                 265                 270         


Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser 
        275                 280                 285             


Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Thr Asp Arg Ser 
    290                 295                 300                 


Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn 
305                 310                 315                 


<210>  12
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  alpha hemolysin mature protein sequence

<400>  12

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  13
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  alpha hemolysin K237C mutation

<400>  13

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Cys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  14
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  alpha hemolysin N17C mutation

<400>  14

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Cys Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  15
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (1)

<400>  15
gcctcgaatc actccactga accatcctct tgatcttgtg aac                         43


<210>  16
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (2)

<400>  16
tgccataagt attcagtgga gcagcaacat agactctcaa caa                         43


<210>  17
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (3)

<400>  17
gttcacaaga atcgaaacca atgttagtgt agagtgcata agc                         43


<210>  18
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (4)

<400>  18
ccaactggga attggtttcg acaagaggat ggaacttatg gca                         43


<210>  19
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (5)

<400>  19
gcttatgcac agagtcacag aacgggaagc agaaacgtgt gag                         43


<210>  20
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (6)

<400>  20
tcgagcaata atctgtgact cctacactaa caatcccagt tgg                         43


<210>  21
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (7)

<400>  21
ctcacacgtt agatacggac acttggatac ggaaaagcac ctc                         43


<210>  22
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (8)

<400>  22
cacttcactt atgtccgtat cctgcttccc gtatattgct cga                         43


<210>  23
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (9)

<400>  23
gaggtgcttt atgtcaatcg gagtagccta gcaagcctta gcc                         43


<210>  24
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (10)

<400>  24
gaaacagata accgattgac accgtatcca agaaagtgaa gtg                         43


<210>  25
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (11)

<400>  25
ggctaaggct aaatgagtac cctatgttgc tgagattcga ggc                         43


<210>  26
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original 12-mer scaffold (12)

<400>  26
ttgttgagag aggtactcat tgctaggcta ctatatctgt ttc                         43


<210>  27
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  12-mer scaffold (optimised 7)

<400>  27
ctcacacgtt agatacggac acttggatag cgaaaagcac ctc                         43


<210>  28
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  12-mer scaffold (optimised 10)

<400>  28
gaaacagata accgattgac acgctatcca agaaagtgaa gtg                         43


<210>  29
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  12-mer scaffold with one long arm (11d)

<400>  29
ggctaaggct aaatgagtac cctatgttgc tgagattcga ggcacttact gagc             54


<210>  30
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  12-mer scaffold with one long arm (11f)

<400>  30
tgtttgatgt ttggtgaatg gtatttggtc g                                      31


<210>  31
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  12-mer scaffold with one long arm (11g)

<400>  31
cgaccaaata ccattcacca aacatcaaac agctcagtaa gt                          42


<210>  32
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  14-mer scaffold (11)

<400>  32
ggctaaggct aaatgagtac ctttttctct ttacctcatc ctc                         43


<210>  33
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  14-mer scaffold (12)

<400>  33
ttccgaacga aggtactcat tgctaggcta ctatatctgt ttc                         43


<210>  34
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  14-mer scaffold (13)

<400>  34
gaggatgagg aaagggtggt cctatgttgc tgagattcga ggc                         43


<210>  35
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  14-mer scaffold (14)

<400>  35
ttgttgagag agaccaccct taaagagaaa aaatcgttcg gaa                         43


<210>  36
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  16-mer scaffold (13)

<400>  36
gaggatgagg aaacacaaca cctcctggct ataaatgcaa gat                         43


<210>  37
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  16-mer scaffold (14)

<400>  37
cgcttagacc agtgttgtgt taaagagaaa aaatcgttcg gaa                         43


<210>  38
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  16-mer scaffold (15)

<400>  38
atcttgcatt acatcagcat actatgttgc tgagattcga ggc                         43


<210>  39
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  16-mer scaffold (16)

<400>  39
ttgttgagag atatgctgat gatagccagg agaggtctaa gcg                         43


<210>  40
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  18-mer scaffold (15)

<400>  40
atcttgcatt acatcagcat attgaagaac taaacgacct gta                         43


<210>  41
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  18-mer scaffold (16)

<400>  41
tggacttgta atatgctgat gatagccagg agaggtctaa gcg                         43


<210>  42
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  18-mer scaffold (17)

<400>  42
tacaggtcgt aaagggtggt cctatgttgc tgagattcga ggc                         43


<210>  43
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  18-mer scaffold (18)

<400>  43
ttgttgagag agaccaccct ttagttcttc aaatacaagt cca                         43


<210>  44
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  20-mer scaffold (17)

<400>  44
tacaggtcgt aatccgccat caactaacta caattcattc cta                         43


<210>  45
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  20-mer scaffold (18)

<400>  45
ctacgaaatg agatggcgga ttagttcttc aaatacaagt cca                         43


<210>  46
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  20-mer scaffold (19)

<400>  46
taggaatgaa atggtaaagg tctatgttgc tgagattcga ggc                         43


<210>  47
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  20-mer scaffold (20)

<400>  47
ttgttgagag aacctttacc atgtagttag ttacatttcg tag                         43


<210>  48
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  24-mer scaffold (19)

<400>  48
taggaatgaa atggtaaagg tgatgtacag ataaaacaaa caa                         43


<210>  49
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  24-mer scaffold (20)

<400>  49
gtagaagctg aacctttacc atgtagttag ttacatttcg tag                         43


<210>  50
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  24-mer scaffold (21)

<400>  50
ttgtttgttt atgagtgaga taactgtaag ggatttgaac atc                         43


<210>  51
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  24-mer scaffold (22)

<400>  51
agggtatcaa aatctcactc aatctgtaca tcacagcttc tac                         43


<210>  52
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  24-mer scaffold (23)

<400>  52
gatgttcaaa aaagggtggt cctatgttgc tgagattcga ggc                         43


<210>  53
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  24-mer scaffold (24)

<400>  53
ttgttgagag agaccaccct tcccttacag ttattgatac cct                         43


<210>  54
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  double 12-mer - figure of eight structure (11d2)

<400>  54
ggctaaggct aaatgagtac cctatgttgc tgagattcga ggcgctcagt aagt             54


<210>  55
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Opened Rings (11a)

<400>  55
ggctaaggct aaatgagt                                                     18


<210>  56
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Opened Rings (11b)

<400>  56
accctatgtt gctgagattc gaggc                                             25


<210>  57
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Opened Rings (12a)

<400>  57
ttgttgagag aggt                                                         14


<210>  58
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Opened Rings (12b)

<400>  58
actcattgct aggctactat atctgtttc                                         29


<210>  59
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rings with docking site (11dx)

<400>  59
ggctaaggct aaatgagtac cctatgttgc tgagattcga ggcacttact gagcactatc       60

tgagc                                                                   65


<210>  60
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tags and tagged oligos used for binding to the docking sites 
       (11d')

<400>  60
gctcagatag tgctcagta                                                    19


<210>  61
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tags and tagged oligos used for binding to the docking sites 
       (11d'c30)

<400>  61
cccccccccc cccccccccc cccccccccc atcccatccc gctcagatag tgctcagta        59


<210>  62
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tags and tagged oligos used for binding to the docking sites 
       (11d-toe)

<400>  62
tactgagcac tatctgagcg ggatgggatg gg                                     32


<210>  63
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OC01

<400>  63
tttttgccgt atttttcctc gctttttcct cgctttttcc tcgctttttc ctcgcttttt       60

cctcgctttt tcctcgcttt ttgccgta                                          88


<210>  64
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OC02

<400>  64
tttttctcgc ttttttcctc gctttttcct cgctttttcc tcgctttttc ctcgcttttt       60

cctcgctttt tcctcgcttt ttctcgct                                          88


<210>  65
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OC03

<400>  65
tttttggtgg gtttttcctc gctttttcct cgctttttgg tggg                        44


<210>  66
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OC04

<400>  66
ttttttcctc ctttttaccc ctttttcctc gctttttacc cctttttcct cgctttttac       60

ccctttttcc tcgctttttc accc                                              84


<210>  67
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OC05

<400>  67
tttttccact ctttttaccc ctttttcctc gctttttacc cctttttcct cgctttttac       60

ccctttttcc tcgctttttc ccgc                                              84


<210>  68
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OC06

<400>  68
tttttcacgt ctttttaccc ctttttcctc gctttttccc ct                          42


<210>  69
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OC07

<400>  69
ttttttcctc ctttttaccc ctttttcctc gctttttacc cctttttcct cgctttttac       60

ccctttttcc tcgctttttg ccga                                              84


<210>  70
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OC08

<400>  70
tttttagccg ctttttaccc ctttttcctc gctttttacc cctttttcct cgctttttac       60

ccctttttcc tcgctttttc accc                                              84


<210>  71
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OC09

<400>  71
tttttcccac atttttaccc ctttttcctc gctttttacc cctttttcct cgctttttcc       60

cgc                                                                     63


<210>  72
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cap01

<400>  72
attgacccac caaaaatacg gc                                                22


<210>  73
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cap02

<400>  73
aagtaagcga gaaaaaccca cc                                                22


<210>  74
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cap03

<400>  74
agatatacgg caaaaaagcg ag                                                22


<210>  75
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cap04

<400>  75
attgaggagg aaaaaaaggg gaaaaa                                            26


<210>  76
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cap05

<400>  76
aagtagagtg gaaaaagggt gaaaaa                                            26


<210>  77
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cap06

<400>  77
agatagacgt gaaaaagcgg gaaaaa                                            26


<210>  78
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cap07

<400>  78
aagtagacgt gaaaaagggt gaaaaa                                            26


<210>  79
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cap08

<400>  79
agtatgcggc taaaaatcgg caaaaa                                            26


<210>  80
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cap09

<400>  80
tattgtgtgg gaaaaagggt gaaaaa                                            26


<210>  81
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CapComp01

<400>  81
gccgtatttt tggtgggtca at                                                22


<210>  82
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CapComp02

<400>  82
ggtgggtttt tctcgcttac tt                                                22


<210>  83
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CapComp03

<400>  83
ctcgcttttt tgccgtatat ct                                                22


<210>  84
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CapComp04

<400>  84
tttttcccct ttttttcctc ctcaat                                            26


<210>  85
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CapComp05

<400>  85
tttttcaccc tttttccact ctactt                                            26


<210>  86
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CapComp06

<400>  86
tttttcccgc tttttcacgt ctatct                                            26


<210>  87
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CapComp07

<400>  87
tttttcaccc tttttcacgt ctactt                                            26


<210>  88
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CapComp08

<400>  88
tttttgccga tttttagccg catact                                            26


<210>  89
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CapComp09

<400>  89
tttttcaccc tttttcccac acaata                                            26


<210>  90
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC01a

<400>  90
aaaaagcgag ttgttgttgt tgaataa                                           27


<210>  91
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC01b

<400>  91
aaaaagcgag gttgttgttg ttgaataa                                          28


<210>  92
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC02a

<400>  92
aaaaatacgg ttgttgttgt tgaataa                                           27


<210>  93
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC02b

<400>  93
aaaaatacgg cttgttgttg ttgaataa                                          28


<210>  94
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC03a

<400>  94
aaaaaagcga ttgttgttgt tgaataa                                           27


<210>  95
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC03b

<400>  95
aaaaaagcga gttgttgttg ttgaataa                                          28


<210>  96
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC04a

<400>  96
aaaaacccac ttgttgttgt tgaataa                                           27


<210>  97
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC04b

<400>  97
aaaaacccac cttgttgttg ttgaataa                                          28


<210>  98
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC05

<400>  98
aaaaaggggt ttgttgttgt tgaataa                                           27


<210>  99
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC06a

<400>  99
aaaaagcgag gttgttgttg ttgaataa                                          28


<210>  100
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC06b

<400>  100
aaaaagcgag ttgttgttgt tgaataa                                           27


<210>  101
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC07a

<400>  101
aaaaaggagg attgttgttg ttgaataa                                          28


<210>  102
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC07b

<400>  102
aaaaaggagg ttgttgttgt tgaataa                                           27


<210>  103
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC08

<400>  103
aaaaagggtg ttgttgttgt tgaataa                                           27


<210>  104
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC09

<400>  104
aaaaaagggg ttgttgttgt tgaataa                                           27


<210>  105
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC010a

<400>  105
aaaaagacgt gttgttgttg ttgaataa                                          28


<210>  106
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC010b

<400>  106
aaaaagacgt ttgttgttgt tgaataa                                           27


<210>  107
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC011

<400>  107
aaaaagcggg ttgttgttgt tgaataa                                           27


<210>  108
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC012a

<400>  108
aaaaagagtg gttgttgttg ttgaataa                                          28


<210>  109
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC012b

<400>  109
aaaaagagtg ttgttgttgt tgaataa                                           27


<210>  110
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC013

<400>  110
aaaaatcggc ttgttgttgt tgaataa                                           27


<210>  111
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC014b

<400>  111
aaaaagcggc ttgttgttgt tgaataa                                           27


<210>  112
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC015

<400>  112
aaaaagggtg ttgttgttgt tgaataa                                           27


<210>  113
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IC016b

<400>  113
aaaaatgtgg ttgttgttgt tgaataa                                           27


<210>  114
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssDNA tail'

<400>  114
ttattcaaca acaa                                                         14


<210>  115
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of lower part of alphaHL beta-barrel inserted into the 
       membrane (1)

<400>  115

Gly Phe Asn Gly Asn Val Thr 
1               5           


<210>  116
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of lower part of alphaHL beta-barrel inserted into the 
       membrane (2)

<400>  116

Gly Gly Leu Ile Gly Ala Asn Val 
1               5               


<210>  117
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  alpha hemolysin (N17C, T115H) mutations

<400>  117

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Cys Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser His Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  118
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  alpha hemolysin (N17C, T145H) mutations

<400>  118

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Cys Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


His Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


